Standard name | |
---|---|
Human Ortholog | |
Description | Major exo-1,3-beta-glucanase of the cell wall; involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; EXG1 has a paralog, SPR1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 5 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 4 | 3 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 45 | 25 | 64 | 60 | 40 | 20 | 60 | 88 | 146 | 168 | 115 | 128 | 138 | 245 | 326 | 42 | 40 | 37 | 6 | 2 | 3 | 11 | 12 | 12 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 4 | 1 | 2 | 0 | 3 | 2 | 3 | 1 | 7 | 1 | 1 | 10 | 7 | 11 | 0 | 0 | 1 | 0 | 0 | 1 |
Endosome | 0 | 0 | 3 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 3 | 9 | 4 | 7 | 7 | 5 | 1 | 8 | 19 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
Mitochondria | 90 | 16 | 15 | 7 | 10 | 10 | 14 | 13 | 18 | 39 | 18 | 11 | 0 | 2 | 1 | 3 | 4 | 5 | 2 | 2 | 3 | 3 | 6 | 7 |
Nucleus | 18 | 16 | 43 | 11 | 38 | 36 | 102 | 114 | 94 | 130 | 23 | 47 | 19 | 6 | 6 | 86 | 89 | 76 | 1 | 3 | 0 | 2 | 4 | 6 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 3 | 3 | 1 | 1 | 3 | 1 | 0 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 3 |
Nucleolus | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 2 |
Vac/Vac Membrane | 104 | 81 | 99 | 48 | 125 | 69 | 147 | 239 | 275 | 337 | 132 | 180 | 59 | 59 | 35 | 86 | 116 | 88 | 168 | 106 | 173 | 158 | 275 | 296 |
Unique Cell Count | 185 | 111 | 174 | 106 | 174 | 113 | 251 | 350 | 401 | 514 | 214 | 312 | 202 | 291 | 355 | 183 | 215 | 184 | 195 | 127 | 197 | 186 | 321 | 359 |
Labelled Cell Count | 261 | 138 | 225 | 131 | 226 | 139 | 329 | 458 | 541 | 685 | 295 | 373 | 228 | 318 | 375 | 232 | 271 | 221 | 195 | 127 | 197 | 186 | 321 | 359 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 5.7 | 5.1 | 5.3 | 6.8 | 7.3 | 7.3 | 7.0 | 7.1 | 7.0 | 6.6 | 6.5 | 5.9 | 5.7 | 5.3 | 7.2 | 7.2 | 6.9 | 6.6 | 6.6 | 6.8 |
Std Deviation (1e-4) | 0.8 | 1.3 | 1.3 | 2.3 | 1.8 | 2.3 | 2.5 | 2.3 | 2.4 | 2.2 | 2.2 | 2.1 | 1.1 | 1.2 | 1.5 | 1.5 | 1.8 | 1.9 | 1.7 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.42 | 0.53 | 0.52 | 0.48 | 0.48 | 0.47 | 0.39 | 0.36 | 0.23 | 0.16 | 0.08 | 0.5 | 0.5 | 0.44 | 0.38 | 0.39 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.2 | -2.8 | -3.5 | -2.9 | -2.8 | -0.1 | -1.0 | 3.3 | 0.9 | 6.1 | 10.5 | 13.5 | -2.9 | -4.0 | -3.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.6 | -1.0 | 0.1 | -1.2 | -2.4 | -2.0 | -0.4 | -0.1 | -2.4 | -4.3 | -4.4 | -5.3 | -3.0 | -3.1 | -2.4 |
Nucleus | -2.9 | -0.6 | 1.3 | 3.4 | 1.8 | -0.3 | 0.2 | -3.6 | -2.6 | -4.0 | -7.7 | -8.6 | 4.4 | 3.5 | 3.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.9 | 2.9 | 0.7 | 0.3 | 2.6 | 2.7 | 2.1 | 1.0 | 0.2 | -5.4 | -8.1 | -11.7 | -1.9 | -0.6 | -1.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4242 | 3.2531 | 2.4866 | 2.0204 | 2.1704 | 2.7246 | 1.2938 | 2.3103 | 1.8234 | 2.7852 | 1.9796 | 2.1776 | 0.1792 | 1.7776 | 1.2295 | 1.5268 | 1.7651 | 1.5309 |
Actin | 0.018 | 0.0001 | 0.0083 | 0 | 0.0068 | 0.0034 | 0.0284 | 0.0004 | 0.0007 | 0.0002 | 0.0062 | 0.0007 | 0.0094 | 0 | 0.0005 | 0 | 0.005 | 0.0005 |
Bud | 0.0004 | 0.0005 | 0.0006 | 0.0003 | 0.0005 | 0.0008 | 0.0007 | 0.0007 | 0.0009 | 0.0003 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0003 |
Bud Neck | 0.0013 | 0.0013 | 0.0012 | 0.0009 | 0.0014 | 0.002 | 0.0019 | 0.0025 | 0.0018 | 0.001 | 0.0018 | 0.0028 | 0.0009 | 0.0011 | 0.0011 | 0.0007 | 0.0008 | 0.0016 |
Bud Periphery | 0.0008 | 0.001 | 0.0014 | 0.0009 | 0.0017 | 0.0026 | 0.0018 | 0.0016 | 0.0015 | 0.0008 | 0.0011 | 0.0016 | 0.0009 | 0.0009 | 0.0009 | 0.0008 | 0.0008 | 0.0009 |
Bud Site | 0.0026 | 0.0038 | 0.0022 | 0.001 | 0.002 | 0.0018 | 0.0044 | 0.0047 | 0.0027 | 0.0008 | 0.0011 | 0.001 | 0.0007 | 0.0017 | 0.0016 | 0.0007 | 0.0012 | 0.0008 |
Cell Periphery | 0.0016 | 0.0036 | 0.0025 | 0.001 | 0.0028 | 0.0037 | 0.0019 | 0.0033 | 0.0026 | 0.0016 | 0.0027 | 0.0033 | 0.0015 | 0.0032 | 0.0017 | 0.0017 | 0.001 | 0.0029 |
Cytoplasm | 0.0134 | 0.0182 | 0.009 | 0.0032 | 0.0021 | 0.0087 | 0.0209 | 0.017 | 0.0096 | 0.0066 | 0.0096 | 0.0047 | 0.0078 | 0.009 | 0.0066 | 0.0008 | 0.0046 | 0.0063 |
Cytoplasmic Foci | 0.0282 | 0.0071 | 0.015 | 0.0075 | 0.0176 | 0.0136 | 0.012 | 0.0091 | 0.0121 | 0.0026 | 0.0073 | 0.0069 | 0.013 | 0.0091 | 0.0097 | 0.002 | 0.0184 | 0.0075 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0046 | 0.0039 | 0.0025 | 0.0009 | 0.0017 | 0.0032 | 0.0098 | 0.0034 | 0.0025 | 0.0031 | 0.0052 | 0.0022 | 0.003 | 0.0019 | 0.0031 | 0.0006 | 0.0008 | 0.0017 |
Endosome | 0.2032 | 0.1052 | 0.1918 | 0.2331 | 0.1437 | 0.1506 | 0.2293 | 0.128 | 0.2123 | 0.1357 | 0.1102 | 0.1347 | 0.2216 | 0.1135 | 0.2049 | 0.1658 | 0.1416 | 0.152 |
Golgi | 0.0141 | 0.0013 | 0.0095 | 0.0065 | 0.0123 | 0.0072 | 0.0169 | 0.0023 | 0.0159 | 0.0031 | 0.0155 | 0.004 | 0.0088 | 0.0019 | 0.0057 | 0.0034 | 0.0032 | 0.003 |
Lipid Particles | 0.0404 | 0.0033 | 0.0265 | 0.0055 | 0.0403 | 0.0211 | 0.0164 | 0.0098 | 0.0143 | 0.0058 | 0.0111 | 0.0081 | 0.0191 | 0.005 | 0.0114 | 0.0039 | 0.0183 | 0.0166 |
Mitochondria | 0.0148 | 0.0013 | 0.0578 | 0.0117 | 0.0051 | 0.0149 | 0.0365 | 0.0112 | 0.0258 | 0.0482 | 0.0199 | 0.0034 | 0.0117 | 0.0053 | 0.0307 | 0.0022 | 0.0106 | 0.0022 |
None | 0.0769 | 0.0113 | 0.0279 | 0.0038 | 0.0012 | 0.0051 | 0.0085 | 0.0079 | 0.0076 | 0.0001 | 0.0076 | 0.0053 | 0.0085 | 0.0086 | 0.0034 | 0.0001 | 0.0051 | 0.0005 |
Nuclear Periphery | 0.013 | 0.0035 | 0.0042 | 0.0017 | 0.0035 | 0.0102 | 0.029 | 0.0056 | 0.0062 | 0.0029 | 0.0204 | 0.0095 | 0.0096 | 0.0105 | 0.0052 | 0.0014 | 0.0105 | 0.0095 |
Nucleolus | 0.0035 | 0.0027 | 0.0068 | 0.0038 | 0.0029 | 0.0041 | 0.0044 | 0.0104 | 0.008 | 0.0026 | 0.0025 | 0.0057 | 0.0034 | 0.0068 | 0.0045 | 0.0026 | 0.0025 | 0.0048 |
Nucleus | 0.0073 | 0.004 | 0.0056 | 0.0052 | 0.0038 | 0.0058 | 0.0139 | 0.0102 | 0.0149 | 0.0311 | 0.0079 | 0.0085 | 0.0052 | 0.0088 | 0.0076 | 0.0028 | 0.022 | 0.0124 |
Peroxisomes | 0.0117 | 0.0002 | 0.0023 | 0.0011 | 0.0258 | 0.0045 | 0.0116 | 0.0007 | 0.0032 | 0.0008 | 0.0016 | 0.0031 | 0.003 | 0.0004 | 0.0015 | 0.0004 | 0.0092 | 0.0007 |
Punctate Nuclear | 0.0085 | 0.0001 | 0.0006 | 0.0002 | 0.0045 | 0.0015 | 0.0039 | 0.0003 | 0.0017 | 0.0001 | 0.0009 | 0.0015 | 0.0009 | 0.0002 | 0.0005 | 0 | 0.0271 | 0.0032 |
Vacuole | 0.4182 | 0.6895 | 0.4958 | 0.5987 | 0.6199 | 0.6159 | 0.4375 | 0.6624 | 0.5481 | 0.6367 | 0.6732 | 0.6729 | 0.5033 | 0.649 | 0.5557 | 0.6459 | 0.5663 | 0.6221 |
Vacuole Periphery | 0.117 | 0.1381 | 0.1283 | 0.113 | 0.1001 | 0.1193 | 0.11 | 0.1084 | 0.1075 | 0.116 | 0.0934 | 0.1197 | 0.1672 | 0.1624 | 0.1433 | 0.1638 | 0.1506 | 0.1506 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 194.9594 | 341.2408 | 172.2517 | 391.9986 | 514.0432 | 153.9163 | 237.6696 | 160.1611 | 316.6733 | 487.0685 |
Translational Efficiency | 1.257 | 1.2788 | 1.0772 | 1.1089 | 1.0526 | 1.2514 | 1.3001 | 1.0769 | 1.0838 | 1.149 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
93 | 333 | 1042 | 809 | 1888 | 2187 | 527 | 1002 | 1981 | 2520 | 1569 | 1811 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 794.40 | 861.38 | 860.48 | 846.09 | 742.48 | 783.96 | 900.90 | 871.21 | 744.92 | 794.19 | 874.06 | 859.99 |
Standard Deviation | 111.51 | 111.05 | 101.64 | 121.08 | 98.73 | 101.51 | 113.05 | 131.46 | 99.98 | 106.11 | 107.32 | 127.54 |
Intensity Change Log 2 | — | 0.116784 | 0.115276 | 0.090946 | — | 0.078428 | 0.279015 | 0.230668 | — | 0.098381 | 0.196702 | 0.160138 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000320 | 0.000076 | 0.000128 | 0.000612 | 0.000024 | 0.000078 | 0.000260 | 0.001085 | 0.000038 | 0.000077 | 0.000172 | 0.000874 |
Bud Neck | 0.023602 | 0.021886 | 0.039160 | 0.009589 | 0.018580 | 0.015405 | 0.012713 | 0.011761 | 0.018816 | 0.016262 | 0.030277 | 0.010791 |
Bud Site | 0.056851 | 0.018650 | 0.010871 | 0.031494 | 0.006740 | 0.013761 | 0.013494 | 0.058019 | 0.009092 | 0.014407 | 0.011752 | 0.046170 |
Cell Periphery | 0.000579 | 0.000419 | 0.000165 | 0.000128 | 0.000786 | 0.000604 | 0.000161 | 0.000188 | 0.000777 | 0.000579 | 0.000164 | 0.000161 |
Cytoplasm | 0.097244 | 0.049589 | 0.129268* | 0.382475* | 0.036647 | 0.030540 | 0.309168* | 0.348275* | 0.039492 | 0.033057 | 0.189693* | 0.363552* |
Cytoplasmic Foci | 0.040989 | 0.030092 | 0.027089 | 0.047293 | 0.012693 | 0.013787 | 0.039830 | 0.058916 | 0.014021 | 0.015942 | 0.031368 | 0.053724 |
Eisosomes | 0.000070 | 0.000034 | 0.000065 | 0.000058 | 0.000030 | 0.000018 | 0.000073 | 0.000073 | 0.000031 | 0.000020 | 0.000068 | 0.000066 |
Endoplasmic Reticulum | 0.004458 | 0.000952 | 0.004867 | 0.002204 | 0.003123 | 0.002282 | 0.004257 | 0.002357 | 0.003186 | 0.002106 | 0.004662 | 0.002289 |
Endosome | 0.074357 | 0.089459 | 0.085899 | 0.019194 | 0.048367 | 0.070705 | 0.042382 | 0.031538 | 0.049587 | 0.073183 | 0.071282 | 0.026024 |
Golgi | 0.002576 | 0.000836 | 0.000576 | 0.010134 | 0.001032 | 0.002615 | 0.000267 | 0.006633 | 0.001105 | 0.002380 | 0.000472 | 0.008197 |
Lipid Particles | 0.016865 | 0.001221 | 0.003628 | 0.002971 | 0.001961 | 0.001186 | 0.004689 | 0.002184 | 0.002661 | 0.001191 | 0.003985 | 0.002536 |
Mitochondria | 0.011201 | 0.000363 | 0.001047 | 0.002727 | 0.000907 | 0.005572 | 0.000398 | 0.003263 | 0.001391 | 0.004884 | 0.000829 | 0.003023 |
Mitotic Spindle | 0.001561 | 0.000193 | 0.003809 | 0.043176 | 0.000380 | 0.001312 | 0.003779 | 0.043293 | 0.000435 | 0.001164 | 0.003799 | 0.043240 |
None | 0.002858 | 0.000066 | 0.002743 | 0.004250 | 0.000228 | 0.000340 | 0.003108 | 0.003100 | 0.000352 | 0.000304 | 0.002866 | 0.003614 |
Nuclear Periphery | 0.002408 | 0.000152 | 0.003075 | 0.001840 | 0.000240 | 0.000234 | 0.002551 | 0.002211 | 0.000341 | 0.000224 | 0.002899 | 0.002046 |
Nuclear Periphery Foci | 0.014213 | 0.000333 | 0.002902 | 0.003556 | 0.000575 | 0.000114 | 0.004111 | 0.003087 | 0.001215 | 0.000143 | 0.003308 | 0.003297 |
Nucleolus | 0.012628 | 0.000754 | 0.010255 | 0.002270 | 0.000870 | 0.001071 | 0.003220 | 0.001669 | 0.001422 | 0.001029 | 0.007892 | 0.001938 |
Nucleus | 0.050958 | 0.006187 | 0.256196* | 0.093096 | 0.012582 | 0.007019 | 0.238894* | 0.074976 | 0.014383 | 0.006909 | 0.250385* | 0.083071 |
Peroxisomes | 0.005615 | 0.002884 | 0.005332 | 0.003921 | 0.000708 | 0.001315 | 0.003651 | 0.007619 | 0.000938 | 0.001522 | 0.004767 | 0.005967 |
Vacuole | 0.559577* | 0.753061* | 0.409467* | 0.329237* | 0.841465* | 0.811171* | 0.311899* | 0.333168* | 0.828232* | 0.803492* | 0.376695* | 0.331412* |
Vacuole Periphery | 0.021069 | 0.022793 | 0.003459 | 0.009775 | 0.012062 | 0.020871 | 0.001094 | 0.006584 | 0.012485 | 0.021125 | 0.002665 | 0.008010 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.26 | 1.59 | -1.05 | -4.54 | -3.73 | -2.30 | -6.72 | -5.39 | -4.93 | -2.98 | -1.82 | -8.86 | -7.03 | -6.43 | -4.88 |
Bud Neck | 0.15 | -1.54 | 1.62 | 4.19 | 11.70 | 1.75 | 1.90 | 5.64 | 4.41 | 2.57 | 1.42 | -5.17 | 6.64 | 6.36 | 11.63 |
Bud Site | 2.47 | 3.01 | 2.59 | 0.49 | -2.36 | -4.90 | -2.28 | -6.86 | -4.77 | -5.07 | -3.54 | -1.50 | -6.61 | -4.31 | -5.81 |
Cell Periphery | 1.45 | 4.29 | 4.91 | 6.48 | 3.51 | 2.34 | 11.90 | 12.09 | 8.17 | 0.65 | 2.77 | 12.08 | 12.90 | 9.24 | 2.73 |
Cytoplasm | 2.37 | -2.01 | -10.74 | -22.88 | -16.57 | 1.71 | -21.44 | -30.16 | -30.96 | -2.63 | 1.81 | -23.24 | -40.14 | -41.71 | -15.53 |
Cytoplasmic Foci | 1.16 | 1.59 | 1.61 | 0.49 | 0.06 | -0.61 | -8.08 | -12.28 | -12.05 | -2.53 | -1.14 | -9.02 | -12.08 | -11.18 | -3.03 |
Eisosomes | 2.61 | 0.40 | 0.41 | -4.70 | 0.03 | 5.15 | -10.80 | -12.24 | -19.25 | 1.93 | 4.85 | -12.20 | -13.25 | -22.32 | 0.81 |
Endoplasmic Reticulum | 2.45 | -0.35 | 1.30 | -7.69 | 8.33 | 3.52 | -4.72 | 3.21 | 0.90 | 6.54 | 4.64 | -6.58 | 3.44 | -0.74 | 10.38 |
Endosome | -1.01 | -0.21 | 4.88 | 10.21 | 14.34 | -6.04 | 4.11 | 10.34 | 15.44 | 2.66 | -6.65 | -2.80 | 14.10 | 20.26 | 13.16 |
Golgi | 2.54 | 3.04 | 0.14 | -1.22 | -1.47 | -2.91 | 2.49 | -2.28 | -0.30 | -3.56 | -2.60 | 2.21 | -2.02 | -0.36 | -3.17 |
Lipid Particles | 2.60 | 2.17 | 2.38 | -3.66 | 4.23 | 1.61 | -2.77 | -0.24 | -3.97 | 3.20 | 2.72 | -2.09 | 0.75 | -5.53 | 5.68 |
Mitochondria | 2.54 | 2.38 | 2.38 | -2.35 | 0.13 | -4.82 | 2.35 | -1.82 | 3.97 | -3.39 | -4.01 | 1.90 | 0.19 | 4.15 | -2.03 |
Mitotic Spindle | 1.84 | -1.89 | -6.82 | -7.28 | -6.03 | -1.49 | -2.39 | -7.31 | -7.03 | -4.94 | -1.28 | -3.79 | -10.20 | -9.89 | -8.04 |
None | 2.49 | 0.31 | -0.89 | -7.61 | -2.07 | -1.02 | -6.25 | -2.79 | -2.64 | 0.31 | 0.44 | -6.12 | -5.56 | -5.66 | -0.91 |
Nuclear Periphery | 1.40 | -0.73 | -0.36 | -13.31 | 1.78 | 0.09 | -8.59 | -5.00 | -4.99 | 2.82 | 1.33 | -14.06 | -9.03 | -9.96 | 3.51 |
Nuclear Periphery Foci | 1.91 | 1.39 | 1.46 | -5.97 | 0.55 | 1.45 | -6.88 | -5.01 | -7.15 | 1.81 | 2.33 | -4.34 | -3.93 | -9.57 | 1.19 |
Nucleolus | 2.71 | 0.49 | 2.45 | -1.52 | 7.47 | -0.20 | -3.41 | -1.32 | -1.07 | 2.01 | 1.19 | -8.87 | -0.54 | -1.48 | 7.85 |
Nucleus | 2.91 | -11.34 | -4.49 | -22.48 | 12.66 | 4.08 | -22.97 | -16.64 | -18.19 | 14.98 | 5.07 | -34.65 | -24.93 | -28.41 | 19.95 |
Peroxisomes | 1.27 | 0.39 | 1.93 | 1.72 | 3.93 | -1.77 | -3.43 | -3.24 | -2.70 | -0.89 | -1.67 | -5.62 | -3.14 | -2.31 | 1.70 |
Vacuole | -4.60 | 3.70 | 3.00 | 16.99 | -1.92 | 3.90 | 38.94* | 35.25* | 32.33* | -6.76 | 3.19 | 48.38* | 42.23 | 40.45* | -5.25 |
Vacuole Periphery | -0.35 | 4.28 | 4.35 | 7.39 | 0.74 | -6.81 | 13.78 | 8.56 | 14.71 | -3.09 | -7.00 | 12.56 | 10.17 | 17.07 | -0.75 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|