Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in vesicular transport; mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 0 | 11 | 15 | 28 | 12 | 14 | 40 | 16 | 19 | 21 | 25 | 3 | 3 | 0 | 2 | 4 | 5 | 1 | 0 | 6 | 0 | 2 | 2 |
Bud | 0 | 4 | 1 | 4 | 5 | 9 | 13 | 24 | 26 | 17 | 28 | 27 | 2 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 2 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 1 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 1 | 11 | 11 | 8 | 14 | 16 | 15 | 21 | 13 | 24 | 20 | 7 | 4 | 2 | 11 | 18 | 11 | 1 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 177 | 135 | 245 | 199 | 287 | 276 | 260 | 294 | 198 | 233 | 243 | 234 | 147 | 187 | 236 | 186 | 452 | 187 | 185 | 140 | 236 | 181 | 326 | 310 |
Endoplasmic Reticulum | 19 | 7 | 25 | 19 | 33 | 23 | 22 | 42 | 21 | 27 | 28 | 24 | 16 | 17 | 9 | 12 | 23 | 7 | 11 | 4 | 42 | 23 | 53 | 53 |
Endosome | 1 | 0 | 10 | 16 | 41 | 55 | 59 | 87 | 50 | 55 | 43 | 45 | 0 | 1 | 2 | 5 | 16 | 6 | 6 | 1 | 12 | 1 | 8 | 13 |
Golgi | 0 | 0 | 2 | 9 | 30 | 13 | 5 | 9 | 2 | 6 | 9 | 3 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 1 | 10 | 3 | 9 | 16 |
Mitochondria | 2 | 11 | 13 | 29 | 44 | 145 | 208 | 293 | 274 | 334 | 311 | 433 | 0 | 0 | 0 | 13 | 41 | 13 | 10 | 4 | 31 | 20 | 29 | 26 |
Nucleus | 1 | 0 | 1 | 2 | 1 | 0 | 4 | 7 | 1 | 2 | 2 | 0 | 2 | 1 | 1 | 1 | 4 | 3 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 3 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 10 | 3 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 1 |
Vac/Vac Membrane | 2 | 0 | 9 | 12 | 22 | 21 | 42 | 52 | 27 | 55 | 26 | 33 | 0 | 2 | 3 | 6 | 36 | 9 | 3 | 8 | 7 | 14 | 27 | 25 |
Unique Cell Count | 204 | 145 | 307 | 268 | 443 | 475 | 558 | 737 | 507 | 641 | 585 | 695 | 162 | 207 | 249 | 207 | 525 | 212 | 235 | 166 | 364 | 251 | 473 | 464 |
Labelled Cell Count | 215 | 159 | 328 | 316 | 501 | 571 | 651 | 876 | 642 | 775 | 744 | 855 | 177 | 215 | 253 | 237 | 600 | 241 | 235 | 166 | 364 | 251 | 473 | 464 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.0 | 7.0 | 8.5 | 9.0 | 8.5 | 7.4 | 6.6 | 6.9 | 6.5 | 6.2 | 6.4 | 5.9 | 8.9 | 9.0 | 8.9 | 6.6 | 7.9 | 9.3 | 9.9 | 10.1 | 10.2 |
Std Deviation (1e-4) | 1.2 | 1.0 | 1.3 | 1.6 | 1.4 | 1.4 | 1.4 | 1.4 | 1.5 | 1.3 | 1.5 | 1.2 | 0.9 | 1.0 | 1.0 | 1.1 | 1.4 | 1.4 | 1.2 | 1.4 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.08 | -0.0 | -0.19 | -0.37 | -0.3 | -0.4 | -0.45 | -0.42 | -0.52 | 0.07 | 0.09 | 0.06 | -0.36 | -0.11 | 0.12 | 0.21 | 0.24 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.2 | 1.7 | -0.9 | -0.9 | 1.3 | -0.3 | -0.5 | 0 | 0 | 0 | -1.5 | 0 | -1.9 | -3.0 | -0.8 |
Bud | 0 | 0 | 0 | 0 | 2.8 | 3.7 | 2.5 | 3.6 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.3 | -1.5 | -0.5 | -0.6 | -1.5 | 0.4 | -1.4 | 0.4 | -0.6 | 0.4 | -1.1 | -2.2 | 1.0 | -0.1 | 0.9 |
Cytoplasm | -1.6 | -4.5 | -6.3 | -9.5 | -11.8 | -11.3 | -12.5 | -10.9 | -13.5 | 3.0 | 3.2 | 5.1 | 3.0 | 2.4 | 2.5 |
Endoplasmic Reticulum | -0.5 | -0.3 | -1.9 | -2.6 | -1.5 | -2.4 | -2.5 | -2.0 | -3.2 | 0.6 | 0 | -2.2 | -1.0 | -2.2 | -2.3 |
Endosome | 1.6 | 3.2 | 4.1 | 3.8 | 4.3 | 3.5 | 3.0 | 2.5 | 2.1 | 0 | 0 | -2.0 | -0.6 | -0.2 | -0.3 |
Golgi | 2.4 | 4.1 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.0 | 2.9 | 8.9 | 10.7 | 11.5 | 14.4 | 14.3 | 14.4 | 17.1 | 0 | -3.0 | -3.3 | 1.0 | 2.0 | 1.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.0 | 1.4 | 1.1 | 2.7 | 2.6 | 1.6 | 3.2 | 1.1 | 1.3 | 0 | 0 | -1.4 | 0 | 2.4 | 0.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.797 | 6.6139 | 5.996 | 5.136 | 5.9489 | 5.8242 | 3.7455 | 5.0339 | 4.4487 | 4.1486 | 4.0277 | 4.5397 | 2.9027 | 4.6776 | 4.214 | 4.2018 | 3.0202 | 4.1813 |
Actin | 0.0146 | 0.0082 | 0.0058 | 0.0262 | 0.0008 | 0.003 | 0.0018 | 0.0021 | 0.0253 | 0.0136 | 0.0437 | 0.0028 | 0.0381 | 0.0576 | 0.0178 | 0.0031 | 0.0113 | 0.0014 |
Bud | 0.0009 | 0.0009 | 0.0009 | 0.0012 | 0.0005 | 0.0005 | 0.0002 | 0.0005 | 0.0009 | 0.0008 | 0.0004 | 0.0003 | 0.0012 | 0.0015 | 0.0011 | 0.0016 | 0.0009 | 0.0003 |
Bud Neck | 0.0011 | 0.0008 | 0.0006 | 0.0036 | 0.0005 | 0.002 | 0.0002 | 0.0002 | 0.0011 | 0.0004 | 0.0006 | 0.0009 | 0.0008 | 0.0004 | 0.001 | 0.0005 | 0.0007 | 0.0011 |
Bud Periphery | 0.0006 | 0.0003 | 0.0004 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0.0005 | 0.0004 | 0.0006 | 0.0008 | 0.0002 |
Bud Site | 0.0048 | 0.0074 | 0.0028 | 0.0057 | 0.0004 | 0.0003 | 0.0022 | 0.0017 | 0.0041 | 0.0006 | 0.001 | 0.0002 | 0.001 | 0.0116 | 0.0037 | 0.0015 | 0.0011 | 0.0002 |
Cell Periphery | 0.0005 | 0.0003 | 0.0003 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.5209 | 0.6801 | 0.6169 | 0.509 | 0.4695 | 0.5796 | 0.7542 | 0.6752 | 0.6398 | 0.5718 | 0.5161 | 0.6286 | 0.5044 | 0.774 | 0.5828 | 0.4583 | 0.4033 | 0.6757 |
Cytoplasmic Foci | 0.0842 | 0.0795 | 0.0799 | 0.06 | 0.1329 | 0.0878 | 0.0446 | 0.0326 | 0.0909 | 0.0623 | 0.1093 | 0.0933 | 0.0445 | 0.079 | 0.0745 | 0.1035 | 0.1132 | 0.0622 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0201 | 0.009 | 0.0089 | 0.0092 | 0.0074 | 0.013 | 0.0275 | 0.0078 | 0.0097 | 0.0028 | 0.0107 | 0.0102 | 0.0131 | 0.0022 | 0.0077 | 0.008 | 0.0034 | 0.0149 |
Endosome | 0.2718 | 0.1624 | 0.2219 | 0.2873 | 0.3253 | 0.238 | 0.1345 | 0.1151 | 0.1601 | 0.2587 | 0.2207 | 0.211 | 0.1537 | 0.0404 | 0.2201 | 0.3266 | 0.3127 | 0.1655 |
Golgi | 0.0439 | 0.0256 | 0.0337 | 0.0355 | 0.0429 | 0.0413 | 0.0066 | 0.0287 | 0.0382 | 0.0665 | 0.0643 | 0.0392 | 0.0702 | 0.0106 | 0.0497 | 0.0417 | 0.054 | 0.0286 |
Lipid Particles | 0.0019 | 0.0031 | 0.0005 | 0.0011 | 0.0003 | 0.0005 | 0.0012 | 0.0003 | 0.0014 | 0.0001 | 0.0074 | 0.0002 | 0.0029 | 0.0018 | 0.0029 | 0.0005 | 0.0232 | 0.005 |
Mitochondria | 0.0083 | 0.005 | 0.0077 | 0.0145 | 0.005 | 0.022 | 0.0013 | 0.0059 | 0.0029 | 0.0019 | 0.0057 | 0.0049 | 0.0256 | 0.0018 | 0.0065 | 0.0151 | 0.0162 | 0.0195 |
None | 0.0049 | 0.002 | 0.0021 | 0.001 | 0.0009 | 0.0012 | 0.0069 | 0.1198 | 0.0021 | 0.0009 | 0.0015 | 0.001 | 0.0294 | 0.0023 | 0.0154 | 0.001 | 0.0007 | 0.0014 |
Nuclear Periphery | 0.003 | 0.0009 | 0.0013 | 0.0019 | 0.0007 | 0.0012 | 0.0038 | 0.0008 | 0.0012 | 0.0007 | 0.0014 | 0.0005 | 0.0449 | 0.0007 | 0.0015 | 0.0018 | 0.0026 | 0.001 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0012 | 0.001 | 0.0009 | 0.0011 | 0.0004 | 0.0006 | 0.0012 | 0.0005 | 0.0007 | 0.0005 | 0.0006 | 0.0004 | 0.0114 | 0.0007 | 0.0008 | 0.0008 | 0.0009 | 0.0006 |
Peroxisomes | 0.0017 | 0.0017 | 0.001 | 0.0018 | 0.0017 | 0.0007 | 0.0003 | 0.0001 | 0.0094 | 0.0006 | 0.0073 | 0.001 | 0.0026 | 0.0087 | 0.0021 | 0.0058 | 0.0338 | 0.0155 |
Punctate Nuclear | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0015 | 0.0002 | 0.0471 | 0.0006 | 0.0004 | 0.0004 | 0.001 | 0.0005 |
Vacuole | 0.0109 | 0.0085 | 0.0094 | 0.0302 | 0.0064 | 0.0045 | 0.0107 | 0.006 | 0.0084 | 0.0118 | 0.0039 | 0.0032 | 0.004 | 0.0044 | 0.0085 | 0.0201 | 0.0141 | 0.0036 |
Vacuole Periphery | 0.0041 | 0.0028 | 0.0045 | 0.0084 | 0.0039 | 0.0033 | 0.0019 | 0.002 | 0.0029 | 0.0057 | 0.0034 | 0.0021 | 0.0032 | 0.0011 | 0.0028 | 0.0088 | 0.0057 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.7284 | 18.0259 | 19.5289 | 26.7574 | 20.9154 | 14.5104 | 20.4131 | 24.9716 | 25.4921 | 23.6279 |
Translational Efficiency | 1.7606 | 1.7783 | 1.4653 | 1.3253 | 1.0819 | 1.8205 | 1.344 | 1.1094 | 1.0691 | 1.0167 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
186 | 1316 | 229 | 490 | 1466 | 927 | 500 | 1816 | 1652 | 2243 | 729 | 2306 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1047.57 | 911.80 | 1233.42 | 1167.98 | 785.60 | 914.58 | 1009.93 | 1040.74 | 815.10 | 912.95 | 1080.13 | 1067.78 |
Standard Deviation | 114.66 | 192.16 | 145.45 | 175.50 | 97.70 | 116.25 | 125.96 | 146.81 | 129.65 | 165.08 | 168.19 | 161.95 |
Intensity Change Log 2 | — | -0.200257 | 0.235617 | 0.156969 | — | 0.219314 | 0.362388 | 0.405743 | — | -0.005354 | 0.291314 | 0.268870 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002207 | 0.000547 | 0.002299 | 0.002838 | 0.000224 | 0.000687 | 0.002093 | 0.001530 | 0.000447 | 0.000605 | 0.002158 | 0.001808 |
Bud Neck | 0.005063 | 0.001153 | 0.002836 | 0.003193 | 0.001944 | 0.002399 | 0.002957 | 0.002618 | 0.002295 | 0.001668 | 0.002919 | 0.002740 |
Bud Site | 0.058644 | 0.032617 | 0.060115 | 0.079166 | 0.032107 | 0.039140 | 0.059280 | 0.065598 | 0.035095 | 0.035313 | 0.059543 | 0.068481 |
Cell Periphery | 0.000145 | 0.000116 | 0.000271 | 0.000241 | 0.000210 | 0.000200 | 0.000136 | 0.000236 | 0.000203 | 0.000151 | 0.000178 | 0.000237 |
Cytoplasm | 0.311439* | 0.466568* | 0.463613* | 0.535448* | 0.240979* | 0.352570* | 0.404431* | 0.488810* | 0.248912* | 0.419454* | 0.423022* | 0.498720* |
Cytoplasmic Foci | 0.299344* | 0.325376* | 0.262242* | 0.196032* | 0.450680* | 0.410635* | 0.327163* | 0.250185* | 0.433641* | 0.360612* | 0.306769* | 0.238678* |
Eisosomes | 0.000059 | 0.000017 | 0.000021 | 0.000020 | 0.000062 | 0.000056 | 0.000023 | 0.000022 | 0.000062 | 0.000033 | 0.000022 | 0.000021 |
Endoplasmic Reticulum | 0.001370 | 0.003536 | 0.003038 | 0.000484 | 0.007010 | 0.002504 | 0.000550 | 0.000507 | 0.006375 | 0.003110 | 0.001331 | 0.000502 |
Endosome | 0.106249 | 0.035254 | 0.055499 | 0.025043 | 0.047476 | 0.053741 | 0.067068 | 0.055212 | 0.054093 | 0.042894 | 0.063434 | 0.048801 |
Golgi | 0.011445 | 0.056370 | 0.031751 | 0.011249 | 0.112692* | 0.078922 | 0.039090 | 0.051886 | 0.101293 | 0.065690 | 0.036785 | 0.043251 |
Lipid Particles | 0.004009 | 0.002187 | 0.004110 | 0.005237 | 0.002986 | 0.002632 | 0.003117 | 0.001933 | 0.003101 | 0.002371 | 0.003429 | 0.002635 |
Mitochondria | 0.000498 | 0.002827 | 0.000108 | 0.002656 | 0.022379 | 0.007010 | 0.000304 | 0.001120 | 0.019916 | 0.004556 | 0.000242 | 0.001447 |
Mitotic Spindle | 0.002742 | 0.006003 | 0.019325 | 0.007712 | 0.001624 | 0.001700 | 0.006064 | 0.004756 | 0.001750 | 0.004224 | 0.010230 | 0.005384 |
None | 0.000014 | 0.001545 | 0.000121 | 0.000092 | 0.000834 | 0.000123 | 0.000036 | 0.000164 | 0.000742 | 0.000958 | 0.000063 | 0.000148 |
Nuclear Periphery | 0.000134 | 0.000217 | 0.000145 | 0.000048 | 0.000117 | 0.000045 | 0.000026 | 0.000023 | 0.000119 | 0.000146 | 0.000064 | 0.000028 |
Nuclear Periphery Foci | 0.000568 | 0.002619 | 0.005370 | 0.001590 | 0.000547 | 0.000287 | 0.000455 | 0.000224 | 0.000549 | 0.001655 | 0.001999 | 0.000514 |
Nucleolus | 0.000384 | 0.000332 | 0.000117 | 0.000107 | 0.000236 | 0.000100 | 0.000064 | 0.000073 | 0.000253 | 0.000236 | 0.000081 | 0.000080 |
Nucleus | 0.003217 | 0.004158 | 0.003611 | 0.002804 | 0.002271 | 0.000952 | 0.001109 | 0.001009 | 0.002378 | 0.002833 | 0.001895 | 0.001391 |
Peroxisomes | 0.018971 | 0.001423 | 0.002540 | 0.008227 | 0.001233 | 0.002537 | 0.008140 | 0.005668 | 0.003230 | 0.001883 | 0.006381 | 0.006212 |
Vacuole | 0.168873 | 0.034006 | 0.077151 | 0.114116 | 0.026052 | 0.026692 | 0.072705 | 0.060044 | 0.042132 | 0.030983 | 0.074102 | 0.071534 |
Vacuole Periphery | 0.004626 | 0.023131 | 0.005717 | 0.003696 | 0.048337 | 0.017070 | 0.005190 | 0.008383 | 0.043416 | 0.020626 | 0.005355 | 0.007387 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.50 | -0.16 | -0.25 | -2.42 | -0.16 | -3.58 | -3.16 | -6.29 | -3.42 | 0.92 | -1.21 | -4.05 | -5.23 | -4.37 | 0.94 |
Bud Neck | 5.55 | 2.47 | 2.29 | -9.84 | -0.81 | -1.99 | -2.99 | -3.13 | -1.04 | 1.05 | 3.42 | -2.28 | -2.44 | -7.04 | 0.68 |
Bud Site | 3.80 | -0.29 | -1.72 | -7.69 | -1.62 | -2.69 | -7.12 | -11.95 | -8.61 | -1.53 | -0.13 | -7.42 | -12.08 | -13.08 | -2.06 |
Cell Periphery | 0.93 | -3.78 | -2.31 | -4.19 | 1.58 | 0.31 | 4.20 | -0.32 | -0.44 | -1.47 | 2.95 | 1.50 | -0.49 | -1.46 | -0.97 |
Cytoplasm | -6.43 | -4.98 | -8.50 | -4.06 | -2.76 | -9.78 | -11.04 | -25.35 | -11.34 | -5.24 | -18.88 | -13.62 | -27.78 | -8.53 | -5.57 |
Cytoplasmic Foci | -1.94 | 1.69 | 6.27 | 13.11 | 4.62 | 4.36 | 11.35 | 27.42 | 17.99 | 6.54 | 10.09 | 13.57 | 29.13 | 18.72 | 7.25 |
Eisosomes | 3.50 | 3.19 | 3.37 | -0.53 | 0.67 | 0.76 | 6.92 | 8.25 | 4.27 | 0.38 | 5.09 | 8.03 | 8.99 | 3.00 | 0.51 |
Endoplasmic Reticulum | -2.56 | -2.18 | 1.52 | 4.67 | 4.74 | 4.29 | 6.71 | 6.74 | 4.62 | 0.25 | 3.41 | 5.77 | 6.84 | 6.07 | 4.16 |
Endosome | 6.40 | 4.06 | 7.56 | 4.78 | 5.86 | -2.05 | -4.18 | -2.65 | -0.23 | 2.43 | 4.45 | -2.36 | 2.22 | -2.17 | 3.97 |
Golgi | -12.63 | -4.36 | 0.45 | 16.32 | 5.30 | 7.16 | 16.92 | 15.62 | 6.98 | -3.46 | 9.86 | 17.12 | 16.75 | 8.83 | -2.11 |
Lipid Particles | 0.99 | -0.12 | -1.16 | -3.79 | -1.90 | 0.54 | -0.42 | 2.86 | 1.14 | 3.85 | 1.45 | -0.87 | 0.55 | -1.18 | 1.80 |
Mitochondria | -3.43 | 1.45 | -0.93 | 0.40 | -1.15 | 7.49 | 16.72 | 16.06 | 3.75 | -6.48 | 10.96 | 16.61 | 14.97 | 3.86 | -2.83 |
Mitotic Spindle | -1.40 | -3.63 | -1.75 | -0.90 | 2.48 | -0.15 | -3.80 | -3.66 | -3.25 | 0.83 | -3.93 | -5.51 | -4.35 | -1.39 | 2.73 |
None | -2.14 | -5.92 | -3.63 | 2.01 | 0.49 | 3.36 | 3.79 | 2.93 | -0.39 | -1.35 | -0.49 | 3.62 | 2.95 | 1.90 | -1.17 |
Nuclear Periphery | -0.70 | -0.17 | 0.89 | 1.55 | 2.18 | 2.38 | 3.25 | 3.46 | 1.24 | 1.06 | -0.43 | 1.90 | 3.38 | 2.00 | 2.77 |
Nuclear Periphery Foci | -3.02 | -7.39 | -6.20 | 1.12 | 5.37 | 1.99 | 0.57 | 2.56 | 0.50 | 3.87 | -2.77 | -6.05 | -0.30 | 2.76 | 6.39 |
Nucleolus | 0.03 | 0.83 | 0.81 | 1.30 | -0.19 | 1.80 | 2.52 | 2.46 | 0.72 | -0.62 | -0.01 | 2.43 | 2.45 | 1.58 | -0.02 |
Nucleus | -0.57 | -0.24 | -0.39 | 0.41 | -0.39 | 2.33 | 1.76 | 2.02 | -1.26 | 0.78 | -0.69 | 0.68 | 1.22 | 2.10 | 1.25 |
Peroxisomes | 4.83 | 4.49 | 2.96 | -6.09 | -4.80 | -5.30 | -6.22 | -15.14 | -8.33 | 2.16 | 2.87 | -3.66 | -5.57 | -12.26 | 0.37 |
Vacuole | 9.63 | 5.67 | 2.25 | -13.21 | -5.12 | -0.26 | -8.51 | -12.28 | -11.52 | 2.31 | 4.25 | -6.60 | -9.98 | -16.05 | -0.09 |
Vacuole Periphery | -7.06 | -0.52 | 0.54 | 12.07 | 2.10 | 13.53 | 21.35 | 19.64 | 6.75 | -4.56 | 11.10 | 20.63 | 19.52 | 12.46 | -3.12 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|