Standard name
Human Ortholog
Description Membrane bound guanine nucleotide exchange factor; also known as a GEF or GDP-release factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; thermosensitivity of the cdc25-5 mutant is functionally complemented by human RASGRF1 or by a fragment of human SOS1 comprising the CDC25-related catalytic domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0.07 0.07 0.14 0.07 0.06 0 0.06 0.08 – 0.08 0.1 0.11 0.12 0.11 0.05 – – – 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.99 0.98 0.94 0.88 0.88 – 0.76 0.61 0.69 1.0 0.99 0.99 – – – 0.92 0.89 0.8 0.89 0.87 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0.05
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.21 0.22 0.17 – 0.42 0.47 0.46 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 1 0 0 0 0 1 – 1 0 0 0 0 4 – – – 4 5 5 0 2 2
Bud 1 13 0 0 2 3 2 1 – 2 2 1 3 2 1 – – – 2 4 2 0 4 10
Bud Neck 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 5 4 2 4 7 7
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 37 33 28 9 12 2 13 19 – 13 7 11 27 29 18 – – – 2 2 4 1 3 3
Cytoplasm 493 472 200 135 212 134 181 214 – 123 43 69 218 267 369 – – – 444 439 155 345 334 383
Endoplasmic Reticulum 4 6 1 0 1 2 3 5 – 0 2 1 1 3 2 – – – 9 8 8 15 12 21
Endosome 1 0 0 0 0 0 2 0 – 0 0 3 0 0 0 – – – 2 1 0 2 1 2
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 2 2 0 0 1 2
Mitochondria 1 0 1 3 5 30 46 41 – 68 33 46 0 0 0 – – – 4 4 5 6 6 1
Nucleus 3 4 1 0 1 0 0 0 – 0 1 0 1 0 1 – – – 1 1 0 0 0 0
Nuclear Periphery 0 1 0 0 1 0 0 1 – 0 0 0 0 0 0 – – – 1 0 0 0 0 2
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 1 0 0 0 0
Vac/Vac Membrane 0 1 0 0 3 0 0 0 – 1 0 3 0 0 2 – – – 2 10 4 3 3 5
Unique Cell Count 496 480 202 136 217 142 206 242 161 70 100 219 270 373 486 492 194 390 383 451
Labelled Cell Count 542 532 232 147 238 171 247 282 208 88 134 250 301 397 486 492 194 390 383 451


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 6.4 6.6 6.1 6.3 5.1 5.1 5.6 – 4.7 5.1 4.8 6.8 6.6 6.9 – – – 6.6 7.4 7.7
Std Deviation (1e-4) 0.8 0.9 1.7 1.9 2.3 1.8 2.1 1.8 – 1.7 1.0 1.4 0.9 0.7 0.9 – – – 1.2 1.6 1.7
Intensity Change (Log2) – – – -0.1 -0.07 -0.36 -0.36 -0.22 – -0.48 -0.36 -0.45 0.04 0.01 0.07 – – – 0.01 0.17 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery -2.1 -2.9 -4.0 -2.5 -2.0 – 0 -0.8 0 -0.5 -1.0 -3.8 – – –
Cytoplasm 0.2 -1.0 -2.5 -4.5 -4.4 – -6.8 -8.8 -7.9 0.7 -0.1 -0.1 – – –
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Mitochondria 0 0 6.6 6.9 5.9 – 0 10.2 0 0 0 0 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5124 2.204 1.7967 1.5734 1.9269 1.839 3.8788 4.393 4.4738 4.5358 4.6115 4.5834 2.8859 3.4441 3.9236 3.5745 3.3202 3.7194
Actin 0.0417 0.0137 0.0129 0.0348 0.0383 0.005 0.0237 0.0023 0.0206 0.0134 0.0063 0.0127 0.0002 0.0009 0.0148 0.0499 0.0004 0.002
Bud 0.0015 0.0042 0.0008 0.0009 0.0051 0.0001 0.0008 0.0025 0.0072 0.0015 0.003 0.0026 0.0001 0 0.0007 0.0296 0 0.0003
Bud Neck 0.0014 0.0017 0.0013 0.0006 0.0024 0.0009 0.001 0.0006 0.0026 0.0102 0.0132 0.0018 0.0001 0.0002 0.0054 0.004 0.0003 0.0006
Bud Periphery 0.0019 0.0068 0.0009 0.002 0.0136 0.0002 0.0023 0.003 0.007 0.0027 0.0093 0.0114 0.0002 0 0.0012 0.198 0.0001 0.0027
Bud Site 0.0063 0.0318 0.0171 0.0045 0.0104 0.0003 0.0089 0.0599 0.023 0.0219 0.0143 0.0021 0.0011 0.0006 0.0153 0.0059 0 0.0001
Cell Periphery 0.0023 0.0016 0.0031 0.0074 0.001 0.0011 0.008 0.0045 0.0054 0.0026 0.0035 0.0051 0.0065 0.0012 0.0022 0.0037 0.0004 0.0014
Cytoplasm 0.4751 0.7572 0.7214 0.7551 0.5446 0.7639 0.508 0.6797 0.5647 0.4838 0.3807 0.623 0.6478 0.937 0.6268 0.4665 0.625 0.7455
Cytoplasmic Foci 0.0274 0.009 0.017 0.0161 0.0278 0.012 0.0149 0.0044 0.0214 0.0213 0.0169 0.005 0.0193 0.0022 0.0291 0.0782 0.0016 0.0022
Eisosomes 0.0005 0.0003 0.0003 0.0004 0.0004 0.0001 0.0014 0.0006 0.0006 0.0008 0.0006 0.0003 0.0001 0.0001 0.0004 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0101 0.0047 0.007 0.0124 0.0102 0.0072 0.0066 0.0029 0.005 0.0067 0.0037 0.0035 0.0068 0.0132 0.0081 0.0018 0.0007 0.0033
Endosome 0.018 0.0057 0.0106 0.0144 0.0431 0.0061 0.0111 0.0009 0.0084 0.0122 0.0223 0.0027 0.0194 0.0008 0.0096 0.008 0.0001 0.0006
Golgi 0.0075 0.0018 0.0036 0.0052 0.026 0.002 0.0032 0.0003 0.0034 0.0051 0.0044 0.0012 0.0019 0.0004 0.0117 0.0206 0.0001 0.0002
Lipid Particles 0.0071 0.0014 0.0014 0.0022 0.0226 0.0025 0.0079 0.0004 0.0038 0.0096 0.0035 0.0009 0.0175 0.0004 0.0074 0.0155 0.0001 0.0002
Mitochondria 0.0022 0.0009 0.0012 0.0023 0.021 0.0004 0.0017 0.0003 0.0008 0.0028 0.0408 0.0016 0.0012 0 0.0038 0.0059 0.0001 0.0001
None 0.3504 0.153 0.1922 0.1291 0.1979 0.193 0.3813 0.2338 0.3073 0.3338 0.4524 0.3211 0.1962 0.0413 0.2518 0.0287 0.3705 0.2398
Nuclear Periphery 0.0062 0.0005 0.0009 0.0028 0.003 0.0009 0.0039 0.0004 0.0013 0.0045 0.0014 0.0008 0.0463 0.0005 0.0012 0.0002 0 0.0001
Nucleolus 0.0012 0.0002 0.0002 0.0002 0.0015 0.0001 0.0008 0.0004 0.0004 0.0026 0.002 0.0002 0.002 0 0.001 0.0001 0 0
Nucleus 0.006 0.0008 0.0008 0.0019 0.0031 0.0004 0.0013 0.0009 0.0012 0.0428 0.0029 0.0007 0.0113 0.0004 0.0015 0.0004 0.0001 0.0002
Peroxisomes 0.0066 0.0024 0.0039 0.0025 0.0163 0.0013 0.0031 0.0003 0.0083 0.0106 0.0028 0.0004 0.0014 0 0.0036 0.081 0 0
Punctate Nuclear 0.0225 0.0007 0.0007 0.0033 0.0054 0.0011 0.005 0.0006 0.004 0.0061 0.0023 0.0006 0.0065 0.0001 0.0014 0.0009 0 0.0001
Vacuole 0.0036 0.0014 0.0023 0.0016 0.0043 0.0013 0.0043 0.0015 0.0032 0.0046 0.0117 0.0021 0.0059 0.0005 0.0028 0.0006 0.0003 0.0006
Vacuole Periphery 0.0007 0.0001 0.0003 0.0003 0.0018 0.0001 0.0008 0.0001 0.0003 0.0006 0.0021 0.0002 0.0082 0 0.0004 0.0002 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.9008 20.2652 22.8633 32.9606 24.8933 17.8019 31.676 34.3619 30.4591 34.7056
Translational Efficiency 0.4657 0.5757 0.5046 0.3471 0.4115 0.6164 0.3763 0.3313 0.4307 0.3798

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
215 1191 229 549 819 1809 806 2329 1034 3000 1035 2878

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 840.36 777.49 956.36 858.09 564.26 752.37 828.13 914.93 621.67 762.34 856.50 904.09
Standard Deviation 135.65 166.19 103.80 186.20 74.64 85.90 104.49 121.00 144.20 124.76 117.13 137.70
Intensity Change Log 2 — -0.112183 0.186546 0.030121 — 0.415082 0.553497 0.697301 — 0.123220 0.345332 0.336029

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003491 0.001812 0.000442 0.001390 0.001312 0.000459 0.000554 0.000304 0.001765 0.000996 0.000529 0.000511
Bud Neck 0.004499 0.014555 0.001839 0.003863 0.048122 0.027762 0.002025 0.002597 0.039051 0.022519 0.001983 0.002838
Bud Site 0.051802 0.094401 0.014926 0.043828 0.037450 0.022395 0.013273 0.025958 0.040434 0.050981 0.013639 0.029367
Cell Periphery 0.000470 0.073013 0.000336 0.003228 0.004214 0.000230 0.000141 0.000108 0.003435 0.029125 0.000185 0.000703
Cytoplasm 0.351466* 0.506509* 0.568392* 0.529188* 0.547585* 0.548708* 0.716880* 0.740964* 0.506806* 0.531955* 0.684026* 0.700566*
Cytoplasmic Foci 0.106174 0.152100 0.131706 0.051457 0.183845* 0.214368* 0.051982 0.039601 0.167695 0.189647* 0.069622 0.041863
Eisosomes 0.000124 0.000237 0.000096 0.013790 0.004862 0.000394 0.000043 0.000036 0.003877 0.000331 0.000055 0.002660
Endoplasmic Reticulum 0.001965 0.001515 0.001685 0.001769 0.005306 0.000958 0.000950 0.000225 0.004611 0.001179 0.001113 0.000519
Endosome 0.027427 0.016756 0.013908 0.005225 0.015351 0.023505 0.006118 0.002517 0.017862 0.020825 0.007841 0.003033
Golgi 0.024046 0.002367 0.000288 0.002363 0.018313 0.008888 0.000713 0.000737 0.019505 0.006299 0.000619 0.001047
Lipid Particles 0.002060 0.004621 0.002025 0.005979 0.020967 0.005257 0.000929 0.000386 0.017036 0.005005 0.001172 0.001453
Mitochondria 0.010836 0.001979 0.000045 0.000454 0.021163 0.008743 0.000079 0.000392 0.019016 0.006058 0.000071 0.000404
Mitotic Spindle 0.025893 0.001701 0.000226 0.007325 0.001202 0.004638 0.001042 0.006114 0.006336 0.003472 0.000862 0.006345
None 0.000392 0.002780 0.000446 0.043178 0.002431 0.000563 0.001470 0.001618 0.002007 0.001443 0.001243 0.009546
Nuclear Periphery 0.000226 0.000262 0.000269 0.000223 0.000305 0.000138 0.000173 0.000082 0.000289 0.000187 0.000194 0.000109
Nuclear Periphery Foci 0.004866 0.000980 0.009721 0.004137 0.000294 0.000499 0.003474 0.000623 0.001245 0.000690 0.004856 0.001293
Nucleolus 0.000113 0.000570 0.000129 0.000093 0.001280 0.000463 0.000114 0.000087 0.001038 0.000506 0.000117 0.000088
Nucleus 0.021097 0.038512 0.039715 0.030405 0.041567 0.034348 0.049775 0.027466 0.037311 0.036001 0.047549 0.028026
Peroxisomes 0.002520 0.003292 0.000464 0.063314 0.005809 0.005860 0.001359 0.000585 0.005125 0.004840 0.001161 0.012551
Vacuole 0.355724* 0.080112 0.213123* 0.188458 0.034908 0.089619 0.148753 0.149170 0.101615 0.085844 0.162995 0.156665
Vacuole Periphery 0.004808 0.001928 0.000219 0.000333 0.003714 0.002206 0.000154 0.000430 0.003941 0.002096 0.000168 0.000412

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.77 2.46 1.77 1.12 -2.35 6.11 4.74 7.38 2.96 1.89 1.80 4.81 5.35 2.15 0.71
Bud Neck -6.96 2.05 1.12 8.71 -1.46 5.64 14.03 14.01 15.96 -0.94 5.76 13.83 13.75 18.30 -1.32
Bud Site -5.25 3.68 1.60 8.41 -3.15 4.98 8.26 5.84 1.26 -3.60 -3.15 8.96 5.75 9.28 -4.61
Cell Periphery -10.39 0.45 -3.41 10.21 -3.82 8.58 8.79 8.88 10.55 3.52 -8.92 8.72 8.09 10.09 -3.10
Cytoplasm -7.98 -7.50 -6.20 1.62 2.23 -0.10 -10.10 -16.02 -18.18 -2.68 -3.06 -11.82 -16.27 -16.87 -1.86
Cytoplasmic Foci -6.36 -2.39 6.72 18.03 9.64 -4.31 21.76 25.63 39.06 5.21 -4.06 18.21 25.82 42.31 9.65
Eisosomes -3.80 1.25 -3.81 -3.73 -3.82 10.10 10.93 10.95 10.11 3.06 9.98 10.78 5.65 -2.86 -3.75
Endoplasmic Reticulum 0.27 -0.35 0.41 0.28 1.57 5.19 5.24 6.22 3.58 9.33 4.99 5.01 6.19 4.46 7.89
Endosome 1.38 2.50 5.33 10.32 5.92 -5.22 7.36 11.90 19.36 3.49 -2.99 7.38 12.62 22.42 5.81
Golgi 2.51 2.85 2.70 2.24 -1.72 7.57 17.39 17.33 10.25 0.05 7.66 11.88 11.72 10.58 -0.48
Lipid Particles -3.83 -0.50 -1.75 1.33 -1.71 7.16 9.19 9.45 14.93 7.96 6.79 9.03 9.14 12.65 1.40
Mitochondria 1.86 2.49 2.42 3.75 -2.37 5.25 10.77 10.64 6.21 -1.57 6.57 10.85 10.70 6.83 -1.92
Mitotic Spindle 2.91 3.16 2.36 -1.67 -2.70 -2.27 -0.30 -2.80 -0.24 -2.95 0.93 2.36 0.06 -1.31 -3.73
None -3.61 -0.11 -3.71 -3.23 -3.70 2.71 1.43 1.45 -1.78 0.31 0.96 1.44 -2.36 -3.11 -3.39
Nuclear Periphery -0.20 -1.71 -1.63 -0.49 0.19 2.00 0.75 2.17 0.30 8.12 1.33 0.46 1.94 0.46 7.30
Nuclear Periphery Foci 1.25 -2.13 0.15 -7.01 7.27 -1.23 -10.73 -5.07 -0.86 9.67 0.74 -6.23 -0.27 -3.68 12.02
Nucleolus -2.98 -0.67 0.61 3.16 2.95 0.87 1.30 1.34 3.34 3.14 0.71 1.30 1.35 4.58 3.98
Nucleus -3.51 -3.21 -4.09 -1.20 -0.01 1.82 -3.29 1.81 -0.07 9.05 0.43 -4.17 0.46 0.06 7.71
Peroxisomes -1.76 2.47 -4.27 -4.07 -4.54 -0.19 8.61 15.71 9.80 1.15 0.28 9.01 -0.67 -0.77 -3.95
Vacuole 15.76 5.98 3.89 -18.61 -3.03 -16.06 -20.51 -32.61 -20.94 -0.47 3.75 -10.18 -13.60 -27.73 -1.54
Vacuole Periphery 1.08 2.40 2.41 3.02 0.20 4.62 13.46 11.90 8.55 -1.47 3.65 9.93 9.28 6.73 -1.42
Standard name
Human Ortholog
Description Membrane bound guanine nucleotide exchange factor; also known as a GEF or GDP-release factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; thermosensitivity of the cdc25-5 mutant is functionally complemented by human RASGRF1 or by a fragment of human SOS1 comprising the CDC25-related catalytic domain
Localization
Cell Percentages cytoplasm (27%), ER (2%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cdc25

Cdc25


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc25-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available