Standard name
Human Ortholog
Description Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.96 0.93 0.88 0.88 0.8 0.78 0.86 0.8 0.74 1.0 0.99 0.99 0.95 0.89 0.87 0.95 0.92 0.95 0.89 0.87 0.78
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0 0 0.14 0.06 0.12 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0.09 0.15 0.18 0.13 0.14 0.12 0.16 0 0 0 0.05 0.09 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1 3
Bud 0 0 0 1 0 2 0 0 5 0 0 2 0 0 0 0 0 0 4 0 0 0 3 9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 1 1 1 0 8 7 2 6 8 2 0 0 0 2 3 4 0 0 0 0 0 0
Cytoplasm 272 260 167 138 169 136 294 238 273 281 265 252 248 274 286 208 103 118 266 254 165 91 242 200
Endoplasmic Reticulum 1 0 1 0 0 4 0 0 3 2 1 4 0 2 1 1 1 3 0 0 0 0 3 6
Endosome 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 3 1 2 0 3 0 0 5 15
Golgi 2 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 1 0 4 4 0 0 1 1
Mitochondria 1 4 0 0 0 10 3 9 50 19 39 40 0 0 2 3 2 2 1 2 2 2 4 2
Nucleus 0 1 0 1 1 2 2 8 3 5 10 12 0 3 2 10 3 3 0 1 0 0 2 1
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 4 8
Vac/Vac Membrane 3 1 1 5 18 14 52 53 44 47 39 56 5 2 0 11 10 15 2 4 1 3 2 2
Unique Cell Count 277 264 169 144 182 155 336 299 350 327 333 340 249 278 290 220 116 136 282 277 174 102 278 257
Labelled Cell Count 279 268 170 148 189 168 362 317 380 361 364 370 253 281 291 241 124 147 282 277 174 102 278 257


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 6.8 7.6 8.0 11.0 7.7 10.1 9.7 7.9 8.5 7.6 7.5 7.9 8.8 8.8 10.9 12.2 11.8 7.1 7.1 7.4
Std Deviation (1e-4) 1.4 1.5 1.3 1.9 2.6 2.6 2.7 2.4 2.4 2.1 1.9 1.9 2.0 2.0 2.2 2.7 3.5 3.3 1.8 2.0 2.0
Intensity Change (Log2) – – – 0.07 0.52 0.02 0.41 0.34 0.05 0.15 -0.01 -0.03 0.05 0.2 0.21 0.51 0.67 0.62 -0.11 -0.1 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.7 -2.8 -4.0 -4.3 -5.9 -6.2 -4.6 -5.9 -6.9 0.9 -0.2 -0.2 -2.2 -3.7 -4.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5.2 3.2 4.6 4.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.8 3.6 5.1 5.6 4.5 4.9 4.3 5.4 0 0 0 2.5 0 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0789 7.966 6.2935 5.1104 4.397 5.6674 7.1328 8.2342 6.9092 6.1337 6.5673 6.6664 7.4912 10.6584 8.7909 10.1009 8.2773 8.7636
Actin 0.0335 0.0005 0.0119 0.026 0.005 0.0014 0.0252 0.0004 0.01 0.0003 0.0021 0.0029 0.0597 0.0005 0.0024 0.0003 0.0002 0.0042
Bud 0.0006 0.0001 0.0004 0.0008 0.0043 0.0004 0.0054 0.0001 0.0022 0.0001 0.001 0.0002 0.0017 0.0042 0.0069 0.0001 0 0.0001
Bud Neck 0.0012 0.0001 0.0007 0.0016 0.0006 0.001 0.0021 0.0002 0.0007 0.0005 0.0007 0.0011 0.0013 0.0001 0.0004 0.0004 0.0002 0.0015
Bud Periphery 0.0008 0 0.0003 0.0012 0.0029 0.0029 0.0022 0 0.0012 0 0.0005 0.0001 0.0014 0.0007 0.0018 0 0 0
Bud Site 0.0045 0.0002 0.0008 0.003 0.0051 0.0012 0.0011 0.0006 0.0034 0.0001 0.001 0.0002 0.0034 0.0008 0.0091 0.0001 0 0.0001
Cell Periphery 0.0001 0 0.0001 0.0002 0.0001 0.001 0.0002 0 0.0001 0 0.0001 0 0.0002 0.0001 0.0003 0 0 0
Cytoplasm 0.769 0.9859 0.9165 0.7634 0.7963 0.9137 0.8457 0.9728 0.929 0.9525 0.8169 0.9194 0.6383 0.9783 0.8999 0.9342 0.8402 0.9325
Cytoplasmic Foci 0.0401 0.0016 0.0123 0.0147 0.0392 0.003 0.0059 0.002 0.0033 0.0021 0.0029 0.0034 0.0482 0.0019 0.008 0.003 0.001 0.0079
Eisosomes 0.0002 0 0 0.0006 0 0.0004 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0054 0.0007 0.0016 0.0024 0.0027 0.0147 0.0112 0.0015 0.0045 0.0013 0.003 0.0046 0.0206 0.0011 0.0041 0.0009 0.0017 0.0033
Endosome 0.035 0.0004 0.0113 0.0154 0.0461 0.0034 0.0064 0.0008 0.0029 0.0012 0.0029 0.0038 0.0395 0.0002 0.0028 0.0012 0.0003 0.0019
Golgi 0.0071 0.0001 0.0117 0.0164 0.0163 0.0003 0.0011 0.0001 0.0009 0.0001 0.0001 0.0003 0.0062 0 0.001 0.0001 0 0.0003
Lipid Particles 0.0097 0 0.0027 0.0126 0.0358 0.0005 0.0002 0 0.0002 0 0 0.0001 0.0101 0 0.0002 0 0 0
Mitochondria 0.005 0.0001 0.0071 0.0644 0.0054 0.0112 0.002 0.0001 0.0009 0.0001 0.0015 0.0006 0.0036 0.0005 0.002 0.0001 0 0.0004
None 0.0164 0.0035 0.0023 0.0471 0.0083 0.012 0.0128 0.0153 0.0222 0.0309 0.0513 0.0174 0.0158 0.0056 0.0447 0.0023 0.1537 0.022
Nuclear Periphery 0.0231 0.0007 0.0013 0.002 0.0019 0.0033 0.0286 0.0009 0.0058 0.0013 0.0144 0.012 0.043 0.001 0.0041 0.0019 0.0005 0.0018
Nucleolus 0.0005 0 0 0.0004 0.0002 0.0005 0.0002 0 0.0001 0 0.0006 0.0001 0.0021 0.0001 0.0009 0 0 0
Nucleus 0.0111 0.0054 0.012 0.0082 0.0085 0.0253 0.0439 0.0042 0.0102 0.0079 0.095 0.031 0.0243 0.0039 0.0087 0.0547 0.0018 0.0206
Peroxisomes 0.0152 0.0001 0.0051 0.0156 0.0121 0.0001 0.0004 0 0.0004 0 0.0002 0.0001 0.0423 0.0001 0.0004 0.0001 0 0.0016
Punctate Nuclear 0.0182 0.0003 0.0006 0.0017 0.0018 0.0006 0.003 0.0004 0.0011 0.0005 0.0033 0.0018 0.0324 0.0005 0.0014 0.0004 0.0002 0.0015
Vacuole 0.0019 0.0002 0.001 0.0014 0.0058 0.0021 0.0016 0.0003 0.0008 0.0008 0.0017 0.0007 0.0036 0.0003 0.0008 0.0001 0.0001 0.0003
Vacuole Periphery 0.0014 0 0.0002 0.0009 0.0016 0.0008 0.0009 0.0001 0.0003 0.0001 0.0007 0.0003 0.0021 0.0001 0.0003 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 117.3638 77.6544 87.0474 154.0933 143.7801 94.2085 73.087 101.4763 266.6148 365.0438
Translational Efficiency 1.2391 1.4379 1.2484 1.6046 1.8274 1.6111 1.4506 1.5501 1.5308 1.6098

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1301 1031 1779 1922 2012 2803 190 1224 3313 3834 1969 3146

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 743.18 857.56 1259.27 1009.23 745.50 940.42 1003.52 1072.09 744.59 918.14 1234.59 1033.69
Standard Deviation 101.00 137.84 335.68 158.35 101.21 130.45 168.62 184.30 101.14 137.48 332.04 171.68
Intensity Change Log 2 — 0.206526 0.760804 0.441471 — 0.335097 0.428789 0.524146 — 0.272343 0.604069 0.483465

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000240 0.000301 0.000327 0.000536 0.000144 0.000740 0.000945 0.007886 0.000182 0.000622 0.000387 0.003396
Bud Neck 0.006905 0.002665 0.002491 0.003721 0.005175 0.004677 0.003081 0.010429 0.005854 0.004136 0.002548 0.006331
Bud Site 0.011850 0.008287 0.004247 0.021603 0.005596 0.008454 0.005875 0.065705 0.008052 0.008409 0.004404 0.038761
Cell Periphery 0.000259 0.000143 0.000133 0.000299 0.000139 0.000057 0.000343 0.000312 0.000186 0.000080 0.000153 0.000304
Cytoplasm 0.609059* 0.742751* 0.558412* 0.630159* 0.701556* 0.779818* 0.374496* 0.475776* 0.665233* 0.769851* 0.540665* 0.570094*
Cytoplasmic Foci 0.192994* 0.078411 0.005238 0.015102 0.104754 0.056051 0.015627 0.029720 0.139406 0.062063 0.006241 0.020789
Eisosomes 0.000087 0.000024 0.000009 0.000022 0.000035 0.000020 0.000111 0.000068 0.000055 0.000021 0.000019 0.000040
Endoplasmic Reticulum 0.003202 0.001355 0.001249 0.001903 0.000962 0.000245 0.009139 0.002771 0.001842 0.000543 0.002010 0.002241
Endosome 0.005329 0.005632 0.000664 0.004629 0.003669 0.002813 0.005216 0.017162 0.004321 0.003571 0.001103 0.009505
Golgi 0.002471 0.000761 0.000121 0.002740 0.001106 0.001407 0.000220 0.020550 0.001642 0.001233 0.000130 0.009669
Lipid Particles 0.003568 0.001540 0.000515 0.000704 0.001840 0.000488 0.003922 0.001451 0.002519 0.000771 0.000844 0.000995
Mitochondria 0.004815 0.000320 0.000517 0.002758 0.001736 0.001799 0.000384 0.014514 0.002945 0.001401 0.000504 0.007332
Mitotic Spindle 0.000518 0.002965 0.007746 0.081138 0.002295 0.007797 0.001655 0.134159* 0.001597 0.006498 0.007159 0.101767*
None 0.005117 0.008275 0.010441 0.007128 0.006432 0.005705 0.006402 0.010709 0.005916 0.006396 0.010051 0.008521
Nuclear Periphery 0.000293 0.000230 0.000806 0.001272 0.000251 0.000134 0.001655 0.001263 0.000267 0.000160 0.000888 0.001269
Nuclear Periphery Foci 0.000758 0.002112 0.001528 0.003394 0.000623 0.000319 0.008277 0.005920 0.000676 0.000801 0.002180 0.004377
Nucleolus 0.000593 0.000816 0.000182 0.000335 0.000739 0.000427 0.000589 0.001001 0.000682 0.000532 0.000222 0.000594
Nucleus 0.107622 0.090926 0.380180* 0.111381 0.128081 0.092975 0.361916* 0.107950 0.120047 0.092424 0.378417* 0.110046
Peroxisomes 0.001994 0.001432 0.000082 0.000724 0.001089 0.001847 0.000421 0.007712 0.001444 0.001735 0.000115 0.003443
Vacuole 0.040283 0.050449 0.025027 0.109069 0.033215 0.033540 0.199488 0.076780 0.035991 0.038087 0.041862 0.096506
Vacuole Periphery 0.002042 0.000605 0.000085 0.001382 0.000562 0.000686 0.000240 0.008162 0.001143 0.000664 0.000100 0.004020

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.38 -5.35 -2.26 -0.24 2.57 -1.82 -8.44 -2.62 -1.76 -1.55 -1.86 -7.19 -2.85 -1.19 -1.74
Bud Neck 5.43 4.67 2.00 -5.30 -4.39 0.63 1.08 -4.24 -5.03 -4.01 2.96 4.42 -1.98 -5.43 -7.40
Bud Site 2.19 5.10 0.97 -1.25 -4.08 -2.56 -1.44 -9.39 -8.44 -8.77 -0.41 2.95 -7.46 -7.35 -9.73
Cell Periphery 1.54 1.27 2.10 0.54 1.86 1.19 -2.32 -0.17 -5.34 4.87 2.15 -0.16 1.13 -1.71 2.69
Cytoplasm -11.93 12.26 16.96 29.15 5.77 -11.63 21.50 32.79 43.03 -3.69 -17.95 26.21 36.50 54.95 7.44
Cytoplasmic Foci 17.25 34.26 32.89 17.52 -7.35 13.31 26.11 22.50 12.71 -4.74 22.58 44.37 39.86 24.22 -11.43
Eisosomes 9.12 11.18 8.50 -2.87 -14.16 8.26 -9.07 -1.60 -4.30 6.93 11.76 10.36 5.42 -6.20 -4.83
Endoplasmic Reticulum 2.96 2.57 2.84 -0.63 1.03 3.67 -9.86 -3.51 -9.39 8.86 4.91 -2.10 0.65 -9.33 4.69
Endosome -0.53 13.53 6.58 4.26 -7.55 1.98 -2.46 -2.79 -4.09 -0.94 2.04 11.45 0.91 -0.95 -8.25
Golgi 5.82 8.45 4.71 0.35 -1.75 -0.62 3.30 -2.80 -2.51 -3.73 1.38 7.53 -0.76 -1.58 -4.15
Lipid Particles 5.11 8.33 6.99 0.77 -6.18 8.66 -5.09 -2.98 -13.12 3.48 10.26 7.98 4.40 -10.52 -6.65
Mitochondria 4.53 4.21 3.74 -3.92 -2.25 -0.07 3.73 -1.45 -1.41 -2.87 3.01 5.22 1.71 -1.10 -3.19
Mitotic Spindle -2.37 -8.06 -15.83 -14.88 -13.20 -3.63 0.72 -16.33 -15.45 -16.49 -4.50 -6.59 -22.43 -20.79 -19.83
None -1.84 -5.02 -3.42 -0.23 2.34 1.11 3.12 -1.67 -2.53 -3.99 -0.45 -5.34 -3.61 -3.00 1.23
Nuclear Periphery 0.64 -11.37 -20.97 -27.19 -15.33 2.91 -12.93 -16.85 -18.32 -1.47 2.59 -20.53 -29.01 -33.32 -15.31
Nuclear Periphery Foci -8.07 -6.87 -5.90 -2.85 -3.19 3.02 -9.67 -7.33 -7.85 2.78 -1.74 -10.47 -9.30 -8.98 -4.10
Nucleolus -1.07 4.88 -4.54 -2.64 -6.43 2.36 2.88 -4.06 -5.66 -6.04 1.36 6.21 -5.66 -6.26 -9.01
Nucleus 2.61 -43.04 -12.37 -15.01 33.07 9.28 -11.16 -4.86 -11.39 9.25 8.69 -46.52 -12.35 -20.50 36.42
Peroxisomes 1.59 10.60 6.75 3.14 -3.91 -2.56 4.30 -3.04 -1.84 -4.22 -1.36 10.61 -0.47 0.53 -5.37
Vacuole -4.66 -3.92 -33.02 -27.92 -29.76 -1.24 -14.45 -26.24 -25.96 3.71 -3.25 -11.26 -42.84 -41.26 -28.34
Vacuole Periphery 4.38 6.01 5.07 1.93 -2.53 -0.73 6.36 -1.99 -1.75 -2.62 2.94 7.85 1.11 -0.85 -3.33
Standard name
Human Ortholog
Description Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ree1

Ree1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ree1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available