Standard name | |
---|---|
Human Ortholog | |
Description | Verprolin, proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 130 | 148 | 146 | 45 | 107 | 96 | 163 | 144 | 76 | – | 66 | 109 | 100 | 192 | 173 | 107 | 113 | 81 | 177 | 211 | 155 | 112 | 208 | 212 |
Bud | 37 | 56 | 51 | 23 | 47 | 42 | 58 | 70 | 29 | – | 38 | 48 | 14 | 47 | 49 | 22 | 24 | 22 | 11 | 19 | 12 | 17 | 18 | 9 |
Bud Neck | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 11 | 13 | 14 | 13 | 7 | 4 |
Bud Site | 19 | 15 | 28 | 4 | 11 | 14 | 19 | 12 | 12 | – | 13 | 8 | 14 | 6 | 0 | 3 | 2 | 2 | – | – | – | – | – | – |
Cell Periphery | 14 | 15 | 16 | 7 | 6 | 5 | 19 | 11 | 14 | – | 10 | 17 | 31 | 62 | 61 | 23 | 20 | 7 | 0 | 1 | 1 | 0 | 3 | 3 |
Cytoplasm | 28 | 26 | 28 | 11 | 31 | 26 | 46 | 51 | 26 | – | 25 | 21 | 13 | 16 | 25 | 24 | 19 | 23 | 2 | 2 | 4 | 0 | 1 | 1 |
Endoplasmic Reticulum | 13 | 26 | 9 | 3 | 16 | 28 | 55 | 23 | 21 | – | 12 | 26 | 7 | 11 | 38 | 6 | 8 | 5 | 1 | 4 | 1 | 2 | 1 | 6 |
Endosome | 1 | 3 | 4 | 1 | 3 | 0 | 6 | 8 | 2 | – | 2 | 5 | 3 | 1 | 5 | 5 | 3 | 5 | 4 | 14 | 8 | 2 | 5 | 4 |
Golgi | 1 | 5 | 5 | 2 | 1 | 1 | 8 | 14 | 0 | – | 1 | 7 | 2 | 16 | 20 | 88 | 86 | 61 | 1 | 7 | 0 | 2 | 2 | 1 |
Mitochondria | 51 | 85 | 22 | 14 | 12 | 70 | 106 | 67 | 149 | – | 183 | 154 | 45 | 37 | 23 | 12 | 20 | 17 | 4 | 11 | 10 | 0 | 3 | 3 |
Nucleus | 0 | 0 | 3 | 1 | 2 | 0 | 6 | 8 | 3 | – | 7 | 3 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | – | 3 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 39 | 32 | 32 | 18 | 17 | 25 | 26 | 43 | 12 | – | 26 | 25 | 54 | 141 | 109 | 35 | 22 | 15 | 3 | 6 | 23 | 8 | 13 | 6 |
SpindlePole | 2 | 4 | 18 | 4 | 8 | 3 | 10 | 17 | 3 | – | 6 | 4 | 0 | 2 | 0 | 9 | 7 | 6 | 0 | 0 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 3 | 4 | 0 | 1 | 2 | 1 | 16 | 1 | – | 2 | 6 | 0 | 0 | 0 | 2 | 4 | 4 | 2 | 8 | 5 | 1 | 2 | 0 |
Unique Cell Count | 191 | 252 | 205 | 72 | 163 | 175 | 318 | 311 | 217 | 247 | 282 | 146 | 276 | 282 | 197 | 196 | 151 | 225 | 303 | 246 | 162 | 273 | 257 | |
Labelled Cell Count | 336 | 421 | 367 | 133 | 262 | 313 | 525 | 488 | 351 | 397 | 434 | 283 | 532 | 504 | 336 | 337 | 250 | 225 | 303 | 246 | 162 | 273 | 257 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 7.7 | 7.3 | 6.9 | 7.7 | 6.5 | 6.8 | 7.2 | 6.1 | – | 5.5 | 6.5 | 8.6 | 9.4 | 9.7 | 14.3 | 14.7 | 14.4 | 7.5 | 8.2 | 9.3 |
Std Deviation (1e-4) | 1.5 | 2.0 | 1.2 | 1.1 | 1.6 | 1.3 | 1.4 | 1.5 | 1.3 | – | 1.1 | 1.3 | 1.5 | 2.0 | 2.1 | 3.9 | 3.7 | 4.1 | 1.9 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.07 | -0.17 | -0.1 | -0.03 | -0.27 | – | -0.4 | -0.18 | 0.23 | 0.35 | 0.4 | 0.96 | 1.0 | 0.97 | 0.04 | 0.15 | 0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.4 | -1.1 | -3.3 | -4.5 | -5.6 | -7.4 | – | -9.4 | -7.1 | -0.5 | -0.4 | -2.3 | -3.5 | -2.8 | -3.4 |
Bud | 1.2 | 0.9 | -0.2 | -1.8 | -0.6 | -3.0 | – | -2.5 | -2.1 | -3.6 | -2.1 | -2.0 | -3.6 | -3.2 | -2.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -1.9 | -2.1 | -1.8 | -3.0 | -4.1 | -2.8 | – | -3.1 | -4.5 | -1.2 | -4.9 | -6.4 | -4.6 | -4.8 | -4.1 |
Cell Periphery | 0.5 | -1.7 | -2.1 | -0.8 | -2.1 | -0.5 | – | -1.7 | -0.8 | 3.6 | 4.3 | 4.1 | 1.3 | 0.8 | -1.2 |
Cytoplasm | 0.3 | 1.4 | 0.3 | 0.3 | 0.8 | -0.5 | – | -1.2 | -2.2 | -1.4 | -3.0 | -1.7 | -0.4 | -1.2 | 0.4 |
Endoplasmic Reticulum | 0 | 2.1 | 3.8 | 4.4 | 1.4 | 2.1 | – | 0.2 | 2.0 | 0.2 | -0.2 | 3.4 | -0.7 | -0.2 | -0.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0.1 | 1.2 | 0 | – | 0 | 0 | 0 | 1.8 | 2.3 | 10.0 | 9.9 | 9.1 |
Mitochondria | 1.9 | -1.1 | 6.6 | 5.9 | 3.2 | 12.1 | – | 13.5 | 10.0 | 4.7 | 0.9 | -1.0 | -1.7 | -0.2 | 0.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.8 | -1.5 | -0.4 | -2.6 | -0.6 | -3.4 | – | -1.6 | -2.3 | 4.6 | 8.0 | 5.5 | 0.6 | -1.3 | -1.6 |
SpindlePole | -0.9 | -1.4 | -3.0 | -2.8 | -1.5 | -3.5 | – | -3.0 | -3.9 | -3.7 | -4.4 | -5.1 | -1.7 | -2.2 | -1.8 |
Vacuole | 0 | 0 | 0 | 0 | 1.8 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.7834 | 5.4234 | 4.7396 | 3.9054 | 4.157 | 5.4088 | 4.8852 | 4.5906 | 4.2876 | 3.861 | 4.1827 | 4.7397 | 4.6117 | 4.6434 | 4.1215 | 4.3537 | 3.9918 | 4.1095 |
Actin | 0.7735 | 0.6917 | 0.7755 | 0.8071 | 0.7366 | 0.8106 | 0.8698 | 0.7295 | 0.8261 | 0.799 | 0.8791 | 0.8881 | 0.8238 | 0.7056 | 0.8372 | 0.9402 | 0.7605 | 0.8281 |
Bud | 0.001 | 0.0037 | 0.0196 | 0.0099 | 0.0174 | 0.0011 | 0.0017 | 0.0041 | 0.0198 | 0.0262 | 0.0111 | 0.0014 | 0.0017 | 0.0074 | 0.0321 | 0.0139 | 0.0062 | 0.0014 |
Bud Neck | 0.0222 | 0.018 | 0.0111 | 0.0008 | 0.0018 | 0.1518 | 0.007 | 0.0051 | 0.0054 | 0.0017 | 0.0011 | 0.0784 | 0.0209 | 0.0075 | 0.0053 | 0.0058 | 0.0209 | 0.1192 |
Bud Periphery | 0.0022 | 0.0036 | 0.041 | 0.0941 | 0.063 | 0.0079 | 0.0044 | 0.004 | 0.0314 | 0.1057 | 0.0257 | 0.0037 | 0.0025 | 0.0082 | 0.059 | 0.0282 | 0.0286 | 0.0033 |
Bud Site | 0.0407 | 0.2227 | 0.0412 | 0.002 | 0.0072 | 0.0045 | 0.0348 | 0.2121 | 0.054 | 0.0053 | 0.0089 | 0.0031 | 0.0423 | 0.2151 | 0.0413 | 0.0032 | 0.0346 | 0.0031 |
Cell Periphery | 0.0028 | 0.0015 | 0.0019 | 0.001 | 0.0008 | 0.0017 | 0.0012 | 0.0012 | 0.0008 | 0.0038 | 0.0007 | 0.0007 | 0.0018 | 0.0019 | 0.0011 | 0.0002 | 0.0015 | 0.0005 |
Cytoplasm | 0.0039 | 0.0274 | 0.0127 | 0.0005 | 0.0026 | 0.0018 | 0.0054 | 0.0195 | 0.0108 | 0.0124 | 0.0018 | 0.001 | 0.0051 | 0.0198 | 0.0039 | 0.0009 | 0.044 | 0.002 |
Cytoplasmic Foci | 0.0239 | 0.0156 | 0.0176 | 0.0046 | 0.0101 | 0.0024 | 0.0102 | 0.0039 | 0.0055 | 0.0179 | 0.009 | 0.0029 | 0.0237 | 0.0098 | 0.0046 | 0.0009 | 0.0159 | 0.0041 |
Eisosomes | 0.0057 | 0.0017 | 0.0014 | 0.0009 | 0.0023 | 0.0027 | 0.0098 | 0.0008 | 0.001 | 0.001 | 0.0018 | 0.0018 | 0.0065 | 0.0008 | 0.0006 | 0.0006 | 0.0007 | 0.0011 |
Endoplasmic Reticulum | 0.0008 | 0.0002 | 0.0009 | 0.0001 | 0.0004 | 0.0001 | 0.0014 | 0.0002 | 0.0007 | 0.0005 | 0.0009 | 0.0008 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0005 | 0.0007 |
Endosome | 0.0091 | 0.0004 | 0.0076 | 0.024 | 0.002 | 0.0002 | 0.0095 | 0.0022 | 0.0052 | 0.0002 | 0.0173 | 0.0024 | 0.0115 | 0.0011 | 0.0024 | 0.0009 | 0.0195 | 0.0067 |
Golgi | 0.0091 | 0.0015 | 0.0048 | 0.0284 | 0.0096 | 0.0017 | 0.0124 | 0.0087 | 0.0065 | 0.0009 | 0.003 | 0.0049 | 0.0139 | 0.0015 | 0.0038 | 0.002 | 0.0136 | 0.0074 |
Lipid Particles | 0.0221 | 0.0003 | 0.0077 | 0.001 | 0.0055 | 0.0002 | 0.0057 | 0.0002 | 0.0046 | 0.0023 | 0.002 | 0.0004 | 0.0085 | 0.0043 | 0.0037 | 0 | 0.0218 | 0.004 |
Mitochondria | 0.0081 | 0.0003 | 0.0047 | 0.0079 | 0.0713 | 0.0002 | 0.0026 | 0.0032 | 0.0046 | 0.0009 | 0.0009 | 0.0021 | 0.0072 | 0.0021 | 0.0009 | 0.0021 | 0.0255 | 0.0029 |
None | 0.0227 | 0.0026 | 0.019 | 0.0016 | 0.0297 | 0.0056 | 0.0011 | 0.0002 | 0.0051 | 0.0164 | 0.0005 | 0.0018 | 0.0008 | 0.0063 | 0.0002 | 0.0001 | 0.0003 | 0.0002 |
Nuclear Periphery | 0.0036 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.005 | 0.0001 | 0.002 | 0.0002 | 0.0016 | 0.0022 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0018 |
Nucleolus | 0.0018 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0012 | 0.0003 | 0 | 0.0001 | 0.0009 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Nucleus | 0.0045 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0008 | 0.0016 | 0.0001 | 0.0002 | 0.0007 | 0.0016 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0019 |
Peroxisomes | 0.0324 | 0.0085 | 0.0267 | 0.0153 | 0.0384 | 0.0038 | 0.0119 | 0.004 | 0.0147 | 0.0007 | 0.0253 | 0.003 | 0.0246 | 0.0071 | 0.0032 | 0.0007 | 0.0027 | 0.0105 |
Punctate Nuclear | 0.0085 | 0.0002 | 0.0035 | 0.0001 | 0.0004 | 0.0014 | 0.003 | 0.0001 | 0.0004 | 0.0013 | 0.0051 | 0.0006 | 0.0027 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0005 |
Vacuole | 0.0008 | 0.0001 | 0.0014 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0008 | 0.0005 | 0.0018 | 0.002 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0002 | 0.0011 | 0.0003 |
Vacuole Periphery | 0.0007 | 0 | 0.0006 | 0.0004 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0013 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.7139 | 14.872 | 18.7905 | 28.1558 | 11.0765 | 18.4248 | 22.0476 | 23.4524 | 25.1283 | 24.8132 |
Translational Efficiency | 2.7712 | 2.0023 | 1.9157 | 1.4595 | 3.1153 | 2.8346 | 1.4369 | 1.6717 | 1.8388 | 1.5782 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2079 | 1665 | 544 | 1678 | 1687 | 1975 | 2135 | 547 | 3766 | 3640 | 2679 | 2225 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 870.22 | 1036.90 | 1422.10 | 1341.82 | 848.02 | 975.95 | 1281.17 | 1308.26 | 860.28 | 1003.83 | 1309.79 | 1333.57 |
Standard Deviation | 111.79 | 159.61 | 213.68 | 237.37 | 121.45 | 149.55 | 182.24 | 218.87 | 116.74 | 157.19 | 197.37 | 233.41 |
Intensity Change Log 2 | — | 0.252825 | 0.708571 | 0.624739 | — | 0.202709 | 0.595292 | 0.625479 | — | 0.228308 | 0.653774 | 0.625104 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.779861* | 0.831237* | 0.714361* | 0.770247* | 0.794272* | 0.774583* | 0.777069* | 0.760988* | 0.786316* | 0.800497* | 0.764335* | 0.767971* |
Bud Neck | 0.020060 | 0.012131 | 0.028644 | 0.008369 | 0.012274 | 0.015131 | 0.014053 | 0.011375 | 0.016572 | 0.013759 | 0.017016 | 0.009108 |
Bud Site | 0.095595 | 0.071394 | 0.187222* | 0.060561 | 0.062866 | 0.072725 | 0.154314* | 0.086638 | 0.080934 | 0.072116 | 0.160996* | 0.066971 |
Cell Periphery | 0.031826 | 0.025833 | 0.008235 | 0.059247 | 0.014698 | 0.026344 | 0.012927 | 0.027878 | 0.024153 | 0.026110 | 0.011975 | 0.051535 |
Cytoplasm | 0.012114 | 0.005176 | 0.006501 | 0.002752 | 0.010702 | 0.009546 | 0.011765 | 0.001981 | 0.011481 | 0.007547 | 0.010696 | 0.002563 |
Cytoplasmic Foci | 0.012951 | 0.007245 | 0.003880 | 0.000777 | 0.013513 | 0.013052 | 0.003066 | 0.005687 | 0.013203 | 0.010396 | 0.003231 | 0.001984 |
Eisosomes | 0.012858 | 0.018478 | 0.022896 | 0.084937 | 0.008758 | 0.014422 | 0.012620 | 0.044725 | 0.011021 | 0.016277 | 0.014706 | 0.075051 |
Endoplasmic Reticulum | 0.000227 | 0.000377 | 0.004894 | 0.002272 | 0.000397 | 0.000584 | 0.000911 | 0.007635 | 0.000303 | 0.000489 | 0.001720 | 0.003590 |
Endosome | 0.001758 | 0.003841 | 0.006780 | 0.001546 | 0.008823 | 0.008507 | 0.002169 | 0.017502 | 0.004923 | 0.006373 | 0.003106 | 0.005468 |
Golgi | 0.002084 | 0.003420 | 0.004289 | 0.001593 | 0.009354 | 0.013235 | 0.000626 | 0.011312 | 0.005340 | 0.008745 | 0.001370 | 0.003983 |
Lipid Particles | 0.001028 | 0.001093 | 0.002741 | 0.001012 | 0.005408 | 0.003940 | 0.000807 | 0.006254 | 0.002990 | 0.002638 | 0.001200 | 0.002301 |
Mitochondria | 0.003823 | 0.002250 | 0.000938 | 0.002730 | 0.005855 | 0.005768 | 0.001657 | 0.002485 | 0.004733 | 0.004159 | 0.001511 | 0.002670 |
Mitotic Spindle | 0.000118 | 0.000672 | 0.000668 | 0.000800 | 0.000226 | 0.000327 | 0.000471 | 0.002651 | 0.000166 | 0.000485 | 0.000511 | 0.001255 |
None | 0.000497 | 0.000062 | 0.000025 | 0.000049 | 0.000115 | 0.000063 | 0.000024 | 0.000026 | 0.000326 | 0.000063 | 0.000024 | 0.000043 |
Nuclear Periphery | 0.000002 | 0.000003 | 0.000027 | 0.000035 | 0.000003 | 0.000004 | 0.000005 | 0.000058 | 0.000003 | 0.000003 | 0.000010 | 0.000041 |
Nuclear Periphery Foci | 0.000010 | 0.000013 | 0.000133 | 0.000055 | 0.000024 | 0.000018 | 0.000025 | 0.000168 | 0.000017 | 0.000016 | 0.000047 | 0.000083 |
Nucleolus | 0.000003 | 0.000002 | 0.000004 | 0.000004 | 0.000005 | 0.000003 | 0.000001 | 0.000003 | 0.000004 | 0.000002 | 0.000002 | 0.000004 |
Nucleus | 0.000102 | 0.000039 | 0.000179 | 0.000061 | 0.000081 | 0.000154 | 0.000045 | 0.000043 | 0.000093 | 0.000101 | 0.000072 | 0.000056 |
Peroxisomes | 0.024826 | 0.016523 | 0.003534 | 0.001791 | 0.052277 | 0.040528 | 0.006341 | 0.008469 | 0.037123 | 0.029548 | 0.005771 | 0.003432 |
Vacuole | 0.000218 | 0.000170 | 0.003937 | 0.000884 | 0.000266 | 0.000478 | 0.001077 | 0.003091 | 0.000240 | 0.000337 | 0.001658 | 0.001426 |
Vacuole Periphery | 0.000039 | 0.000041 | 0.000112 | 0.000277 | 0.000084 | 0.000589 | 0.000026 | 0.001031 | 0.000059 | 0.000338 | 0.000043 | 0.000463 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.67 | 4.65 | 1.03 | 6.27 | -3.90 | 1.96 | 1.90 | 2.16 | 0.91 | 0.96 | -2.13 | 3.22 | 2.29 | 4.09 | -0.70 |
Bud Neck | 3.74 | -2.42 | 6.62 | 2.51 | 6.35 | -1.37 | -0.94 | 0.41 | 1.70 | 1.33 | 1.86 | -0.26 | 5.82 | 3.86 | 6.03 |
Bud Site | 3.90 | -7.11 | 6.13 | 1.88 | 9.94 | -1.76 | -13.37 | -2.63 | -1.56 | 6.93 | 2.10 | -13.62 | 3.04 | 1.13 | 15.51 |
Cell Periphery | 2.25 | 10.21 | -6.99 | -8.54 | -13.85 | -5.07 | 1.00 | -3.16 | -0.36 | -3.69 | -1.09 | 8.00 | -9.00 | -8.29 | -13.57 |
Cytoplasm | 5.26 | 0.96 | 6.90 | 2.65 | 2.73 | 0.76 | -1.01 | 6.54 | 7.53 | 7.38 | 3.79 | -0.37 | 8.93 | 6.61 | 7.21 |
Cytoplasmic Foci | 3.44 | 5.10 | 9.16 | 6.25 | 3.54 | 0.34 | 6.88 | 4.22 | 4.03 | -1.68 | 2.29 | 8.77 | 11.16 | 9.31 | 1.98 |
Eisosomes | -3.36 | -2.65 | -16.06 | -14.35 | -11.00 | -3.92 | -2.52 | -6.38 | -5.34 | -5.65 | -4.81 | -2.65 | -17.47 | -15.81 | -15.89 |
Endoplasmic Reticulum | -1.40 | -5.64 | -4.82 | -4.47 | 2.82 | -1.23 | -1.80 | -4.56 | -4.44 | -4.19 | -1.95 | -5.03 | -6.50 | -6.10 | -3.27 |
Endosome | -2.71 | -2.90 | 0.41 | 2.96 | 3.01 | 0.21 | 5.12 | -2.57 | -2.69 | -4.73 | -1.71 | 2.21 | -0.57 | 0.83 | -2.30 |
Golgi | -2.11 | -1.50 | 0.87 | 2.59 | 1.79 | -2.21 | 7.60 | -0.79 | 0.73 | -4.76 | -3.62 | 6.39 | 1.58 | 4.72 | -3.69 |
Lipid Particles | -0.14 | -2.32 | 0.02 | 0.20 | 2.51 | 1.09 | 4.05 | -0.44 | -1.30 | -3.35 | 0.51 | 2.87 | 0.98 | 0.52 | -1.99 |
Mitochondria | 1.76 | 3.85 | 1.10 | -0.64 | -2.85 | 0.08 | 4.63 | 2.95 | 2.83 | -1.01 | 0.75 | 5.25 | 2.72 | 2.01 | -2.08 |
Mitotic Spindle | -1.73 | -3.88 | -2.34 | -0.42 | -0.26 | -0.80 | -1.56 | -1.70 | -1.64 | -1.52 | -1.99 | -2.92 | -2.67 | -1.81 | -1.71 |
None | 1.28 | 1.37 | 1.31 | 1.51 | -3.69 | 2.74 | 4.87 | 4.65 | 4.68 | -0.24 | 1.40 | 1.60 | 1.50 | 3.33 | -5.32 |
Nuclear Periphery | -0.76 | -6.00 | -2.74 | -2.72 | -0.34 | -1.08 | -2.31 | -2.93 | -2.88 | -2.78 | -1.37 | -6.02 | -3.77 | -3.70 | -2.95 |
Nuclear Periphery Foci | -0.98 | -3.09 | -1.51 | -1.42 | 1.96 | 0.95 | 0.12 | -2.03 | -2.18 | -2.05 | 0.46 | -2.84 | -2.26 | -2.35 | -0.82 |
Nucleolus | 2.06 | -1.85 | -1.13 | -1.85 | 0.34 | 2.25 | 3.71 | 1.71 | -0.25 | -1.92 | 2.82 | 2.45 | -0.44 | -1.62 | -1.54 |
Nucleus | 2.83 | -2.24 | 0.97 | -1.79 | 2.54 | -0.76 | 2.31 | 3.48 | 1.18 | 0.95 | -0.15 | -0.56 | 1.39 | 0.66 | 1.61 |
Peroxisomes | 3.16 | 9.94 | 11.33 | 8.11 | 1.86 | 2.88 | 13.93 | 11.24 | 9.28 | -0.85 | 3.13 | 16.03 | 17.52 | 14.78 | 2.33 |
Vacuole | 0.52 | -3.57 | -2.45 | -2.70 | 3.11 | -1.47 | -2.19 | -2.50 | -2.30 | -1.34 | -1.07 | -3.98 | -3.47 | -3.17 | 1.45 |
Vacuole Periphery | -0.10 | -2.26 | -2.55 | -2.54 | -1.69 | -1.36 | 2.59 | -2.46 | -0.85 | -2.61 | -1.38 | 0.98 | -3.43 | -0.58 | -3.55 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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