Standard name
Human Ortholog
Description Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes; ATG33 has a paralog, SCM4, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0 0.19 0 0 0 0 0 0
Bud 0 0 0.06 0.07 0 0.06 0.05 0.06 – 0.07 0.09 0.07 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.11 0.12 0 0 0 0 0 0
Cytoplasm 0.6 0.58 0.46 0.45 0.24 0.21 0.2 0.2 – 0.21 0.23 0.22 0.83 0.79 0.83 0.14 0.06 0.12 0.18 0.14 0.1 0.13 0.11 0.1
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0.11 0.17 0.12 0 0 0 0 0 0
Endosome 0 0 0 0.05 0.06 0 0 0 – 0 0 0 0 0.05 0 0.14 0.06 0 0.07 0 0.1 0.06 0.09 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0.11 0.06 0 0 0 0 0 0
Mitochondria 0.28 0.29 0.51 0.54 0.78 0.8 0.84 0.8 – 0.77 0.8 0.83 0 0 0 0.2 0.28 0.19 0.54 0.59 0.62 0.56 0.56 0.58
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.12 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0 0 0 0 – 0 0 0 0 0 0 0.14 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.05 0.05 0 0.06 0.07 – 0.09 0.07 0.06 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.09 0 0 0.05 0 0 0 – 0 0 0 0 0.05 0 0.2 0.39 0.44 0.07 0.11 0 0.09 0.08 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 1 0 0 2 0 0 – 0 0 0 4 3 5 3 0 3 3 7 6 1 2 6
Bud 3 2 11 22 15 29 36 43 – 49 41 44 1 1 2 0 1 0 1 4 0 1 3 5
Bud Neck 4 0 3 0 0 2 1 1 – 3 1 4 1 2 5 0 0 0 2 4 0 0 5 7
Bud Site 0 0 0 0 3 8 9 5 – 13 13 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 1 8 1 1 1 1 – 5 4 3 2 2 8 1 2 2 0 2 1 0 1 1
Cytoplasm 103 122 84 139 104 101 136 153 – 152 105 142 99 138 150 5 1 2 41 40 19 16 17 22
Endoplasmic Reticulum 4 11 7 0 1 2 1 0 – 0 0 4 4 11 3 4 3 2 5 2 1 1 1 0
Endosome 4 7 2 16 25 4 3 6 – 7 2 0 5 9 8 5 1 0 15 11 19 7 14 8
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 3 2 1 0 2 0 3 0 0
Mitochondria 48 61 94 166 342 379 569 608 – 545 371 537 2 5 2 7 5 3 126 174 122 67 85 124
Nucleus 1 1 2 1 3 6 13 20 – 10 6 5 2 1 0 1 0 0 1 1 0 0 0 0
Nuclear Periphery 0 2 0 0 1 0 0 2 – 4 1 1 1 0 0 0 0 2 0 0 0 0 0 0
Nucleolus 0 0 1 0 3 4 7 4 – 8 3 4 0 0 0 0 0 1 0 0 1 0 0 0
Peroxisomes 0 0 1 17 5 1 5 10 – 8 2 1 1 3 2 5 0 0 1 0 2 0 0 1
SpindlePole 1 2 11 16 20 20 41 51 – 63 32 39 2 0 0 2 1 1 6 4 6 3 2 5
Vac/Vac Membrane 9 19 3 4 23 10 18 17 – 30 9 9 5 9 8 7 7 7 16 32 6 10 12 23
Unique Cell Count 171 209 184 308 441 471 679 759 708 466 650 120 174 180 35 18 16 233 295 199 120 154 214
Labelled Cell Count 180 233 221 389 546 569 840 921 897 590 801 129 184 193 43 23 24 233 295 199 120 154 214


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.3 3.5 3.6 3.9 3.6 3.0 3.2 – 3.0 3.2 2.9 5.2 5.0 5.3 6.3 6.4 6.1 5.6 5.3 5.6
Std Deviation (1e-4) 0.5 0.6 1.2 1.2 1.8 1.3 1.1 1.2 – 1.1 1.5 0.9 0.9 0.9 1.1 1.2 1.7 1.0 2.2 1.1 1.2
Intensity Change (Log2) – – – 0.03 0.17 0.07 -0.23 -0.13 – -0.2 -0.13 -0.27 0.58 0.54 0.6 0.86 0.89 0.81 0.68 0.61 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4776 1.1451 1.2053 1.2142 1.3011 1.4986 1.9279 1.6887 1.6064 1.1 1.9705 2.0517 -0.2716 0.3146 -0.1202 0.1635 -0.3226 -0.0094
Actin 0.0286 0.0251 0.0305 0.0018 0.0632 0.0104 0.0398 0.0065 0.0372 0.0018 0.0143 0.008 0.0071 0.0038 0.0033 0.0089 0.0012 0.0052
Bud 0.0027 0.0033 0.0057 0.0027 0.0016 0.0027 0.0015 0.0098 0.0061 0.002 0.0022 0.0013 0.0009 0.0053 0.0021 0.0019 0.0007 0.001
Bud Neck 0.0025 0.002 0.0034 0.0007 0.0028 0.0048 0.0086 0.0015 0.0036 0.0023 0.0022 0.0084 0.0008 0.0012 0.0026 0.0012 0.0005 0.0051
Bud Periphery 0.0031 0.0057 0.0133 0.0136 0.0028 0.0119 0.0044 0.0258 0.0238 0.0133 0.0095 0.0059 0.0029 0.0119 0.0056 0.0057 0.0015 0.003
Bud Site 0.0067 0.045 0.0279 0.0029 0.0036 0.0025 0.0179 0.0298 0.0168 0.0056 0.0037 0.0015 0.0023 0.0217 0.0073 0.0022 0.0008 0.0012
Cell Periphery 0.0019 0.0041 0.0013 0.002 0.0005 0.0011 0.0041 0.0059 0.0031 0.0022 0.0029 0.0019 0.001 0.0016 0.0009 0.0004 0.0003 0.0006
Cytoplasm 0.0351 0.0504 0.0285 0.0289 0.0084 0.0193 0.0309 0.0825 0.0503 0.0128 0.0248 0.0538 0.0164 0.0366 0.0228 0.0651 0.0222 0.0227
Cytoplasmic Foci 0.0771 0.0665 0.0827 0.0586 0.0636 0.0729 0.1148 0.1167 0.082 0.1103 0.127 0.1034 0.0774 0.0909 0.0791 0.0956 0.1226 0.0663
Eisosomes 0.0009 0.0012 0.0008 0.0003 0.0009 0.0008 0.0025 0.0009 0.0019 0.0006 0.0021 0.0008 0.0007 0.0005 0.0004 0.0002 0.0001 0.0003
Endoplasmic Reticulum 0.0083 0.0039 0.0039 0.0024 0.0015 0.0056 0.0045 0.0033 0.0028 0.0013 0.0015 0.0016 0.0037 0.0037 0.0035 0.002 0.0009 0.0013
Endosome 0.1176 0.1019 0.0933 0.1208 0.102 0.0934 0.1115 0.1188 0.1064 0.1021 0.1055 0.1011 0.1701 0.1352 0.1204 0.1035 0.2138 0.1193
Golgi 0.0533 0.0491 0.0472 0.0708 0.0676 0.0617 0.0408 0.0653 0.0368 0.0322 0.1052 0.0377 0.0512 0.0535 0.0446 0.0274 0.0628 0.0461
Lipid Particles 0.051 0.0171 0.0318 0.0058 0.0156 0.0405 0.035 0.0083 0.0157 0.0053 0.0321 0.0171 0.0607 0.0265 0.0212 0.0043 0.0375 0.0209
Mitochondria 0.4661 0.4825 0.4827 0.4866 0.4715 0.5284 0.3047 0.3917 0.3735 0.4823 0.3542 0.4549 0.4778 0.4951 0.5535 0.4643 0.4081 0.5957
None 0.0644 0.0465 0.0731 0.1629 0.0615 0.0785 0.17 0.083 0.1591 0.1445 0.084 0.1404 0.0532 0.0327 0.0582 0.1342 0.0638 0.0411
Nuclear Periphery 0.0063 0.0021 0.0032 0.001 0.0006 0.0032 0.0035 0.0014 0.001 0.0003 0.0004 0.0004 0.0043 0.0008 0.0021 0.0017 0.0005 0.0009
Nucleolus 0.0047 0.0014 0.0032 0.0008 0.0003 0.0011 0.0028 0.0018 0.0018 0.0012 0.0008 0.0006 0.0028 0.0032 0.0019 0.0014 0.0007 0.0007
Nucleus 0.0051 0.0066 0.0027 0.0011 0.0005 0.0021 0.0121 0.0018 0.0017 0.0011 0.0008 0.0008 0.002 0.0026 0.0017 0.0041 0.0007 0.0009
Peroxisomes 0.034 0.0578 0.0403 0.0232 0.1225 0.0475 0.0673 0.0246 0.0573 0.0689 0.1179 0.0478 0.0376 0.0454 0.0477 0.0598 0.0342 0.0527
Punctate Nuclear 0.0041 0.0021 0.0047 0.0005 0.001 0.0027 0.0063 0.0006 0.0017 0.0006 0.0022 0.0006 0.0022 0.0073 0.0013 0.0022 0.0067 0.0009
Vacuole 0.0187 0.0186 0.0149 0.0091 0.0034 0.0054 0.0131 0.0164 0.0122 0.0059 0.0045 0.0084 0.017 0.0154 0.013 0.0095 0.0119 0.0084
Vacuole Periphery 0.0078 0.0071 0.0051 0.0035 0.0045 0.0036 0.0037 0.0037 0.0053 0.0036 0.0022 0.0037 0.008 0.0053 0.007 0.0044 0.0088 0.0059

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.647 9.9634 14.7865 21.445 16.8591 24.1352 15.5307 19.2646 22.7738 22.5169
Translational Efficiency 1.6254 2.146 1.6544 1.56 1.7709 1.6928 2.1617 1.9818 2.2443 1.8029

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
272 1708 1205 120 1775 1245 552 1821 2047 2953 1757 1941

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 583.64 710.55 848.09 911.64 684.20 648.98 892.91 910.33 670.84 684.59 862.17 910.41
Standard Deviation 61.17 91.88 114.35 119.50 79.40 78.63 91.59 132.80 84.44 91.73 109.71 132.01
Intensity Change Log 2 — 0.283857 0.539139 0.643385 — -0.076244 0.384097 0.411972 — 0.100735 0.457544 0.523127

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000129 0.000757 0.002400 0.006865 0.000501 0.000983 0.001382 0.011522 0.000452 0.000852 0.002080 0.011234
Bud Neck 0.043748 0.051436 0.019982 0.013244 0.041054 0.056393 0.021525 0.047449 0.041412 0.053526 0.020467 0.045334
Bud Site 0.004149 0.036934 0.050361 0.031566 0.011681 0.039950 0.017009 0.139349* 0.010680 0.038206 0.039882 0.132686*
Cell Periphery 0.000644 0.000194 0.000183 0.000183 0.000210 0.000322 0.000143 0.000340 0.000268 0.000248 0.000170 0.000330
Cytoplasm 0.184449* 0.154305* 0.298350* 0.274287* 0.216747* 0.101214* 0.361448* 0.228755* 0.212455* 0.131921* 0.318174* 0.231570*
Cytoplasmic Foci 0.394227* 0.275805* 0.156557 0.136241 0.387865* 0.231188* 0.090363 0.144151 0.388710* 0.256994* 0.135761 0.143662
Eisosomes 0.000734 0.000165 0.000078 0.000131 0.000340 0.000260 0.000089 0.000186 0.000392 0.000205 0.000082 0.000183
Endoplasmic Reticulum 0.001325 0.001079 0.007285 0.004098 0.001721 0.000905 0.004177 0.001839 0.001669 0.001005 0.006309 0.001979
Endosome 0.030643 0.072028 0.040910 0.057275 0.054067 0.084290 0.018781 0.030750 0.050955 0.077198 0.033958 0.032390
Golgi 0.017647 0.042154 0.015816 0.014403 0.028368 0.055430 0.001001 0.029798 0.026944 0.047751 0.011162 0.028846
Lipid Particles 0.053847 0.011695 0.005658 0.005127 0.025607 0.013129 0.005538 0.002644 0.029360 0.012299 0.005620 0.002797
Mitochondria 0.121011* 0.159318* 0.022389 0.006508 0.080986 0.268598* 0.002335 0.048061 0.086305 0.205391* 0.016089 0.045492
Mitotic Spindle 0.000415 0.017659 0.051726 0.031994 0.002856 0.005568 0.015613 0.049706 0.002532 0.012561 0.040380 0.048611
None 0.002312 0.002507 0.002666 0.001502 0.001047 0.001079 0.003354 0.000719 0.001215 0.001905 0.002882 0.000767
Nuclear Periphery 0.000957 0.000335 0.001995 0.001455 0.000430 0.000360 0.003355 0.000328 0.000500 0.000346 0.002422 0.000398
Nuclear Periphery Foci 0.000218 0.000310 0.003530 0.003496 0.000305 0.000199 0.002486 0.000382 0.000293 0.000263 0.003202 0.000574
Nucleolus 0.004806 0.002930 0.003877 0.002357 0.001374 0.001302 0.002584 0.000857 0.001830 0.002243 0.003471 0.000950
Nucleus 0.065942 0.035022 0.154956* 0.087760 0.055190 0.015584 0.300350* 0.050827 0.056619 0.026827 0.200635* 0.053110
Peroxisomes 0.043566 0.048325 0.018275 0.039632 0.027812 0.055654 0.012956 0.086360 0.029905 0.051415 0.016604 0.083471
Vacuole 0.024092 0.072924 0.130293 0.273270* 0.054798 0.052498 0.133511 0.122112 0.050718 0.064312 0.131304 0.131457
Vacuole Periphery 0.005139 0.014118 0.012712 0.008605 0.007040 0.015095 0.001999 0.003866 0.006787 0.014530 0.009346 0.004159

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.99 -7.05 -1.72 -1.49 -0.88 -2.04 -2.29 -9.95 -9.48 -8.83 -2.23 -5.68 -10.14 -9.79 -8.28
Bud Neck -1.20 3.92 4.60 9.59 2.09 -3.84 5.71 -1.80 2.52 -7.46 -4.12 8.61 -1.09 3.15 -10.37
Bud Site -15.88 -15.11 -5.23 2.08 4.21 -10.08 -1.86 -26.93* -17.59 -24.41* -15.54 -12.41 -27.17 -19.37 -18.47
Cell Periphery 4.22 4.33 4.51 1.09 1.00 -3.88 4.66 -1.82 0.19 -3.22 0.86 4.76 -0.62 -1.03 -2.57
Cytoplasm 2.22 -7.98 -2.32 -3.65 1.93 16.35 -9.61 -2.26 -18.04 8.02 13.22 -11.92 -3.08 -15.49 8.61
Cytoplasmic Foci 7.98 16.64 14.67 11.40 2.49 19.51 42.01 38.33 13.46 -9.11 20.52 41.59 41.03 22.61 -1.34
Eisosomes 5.15 5.92 5.44 2.29 -3.22 3.33 12.35 7.62 5.08 -9.10 7.92 13.53 9.09 2.83 -11.00
Endoplasmic Reticulum 0.68 -11.78 -3.18 -3.53 2.43 3.97 -6.62 -0.98 -5.04 6.14 3.70 -14.33 -2.04 -5.70 12.71
Endosome -8.35 -1.88 -1.40 2.80 -0.58 -6.83 12.25 9.27 14.21 -4.99 -8.38 7.06 8.37 18.18 1.36
Golgi -8.73 1.30 1.70 6.54 1.13 -8.45 17.01 1.03 8.99 -14.10 -9.46 9.81 0.94 9.62 -7.56
Lipid Particles 7.91 9.18 9.29 5.60 1.03 6.58 13.44 16.75 7.75 3.71 10.65 16.75 19.04 11.22 6.86
Mitochondria -2.60 7.81 9.03 24.54* 5.28 -20.09* 21.11 8.35 25.20 -15.18 -19.07 18.32 10.36 29.35 -8.89
Mitotic Spindle -9.69 -12.45 -2.91 -1.43 1.26 -2.68 -4.35 -14.51 -13.33 -8.04 -8.43 -12.47 -14.87 -11.02 -2.08
None -0.29 -0.32 0.74 1.26 1.55 -0.16 -4.47 1.85 1.53 5.14 -2.21 -5.83 2.31 3.54 7.67
Nuclear Periphery 1.62 -2.69 -1.57 -4.19 0.85 0.46 -4.50 -0.16 -0.52 4.49 1.68 -7.93 -0.27 -2.29 8.01
Nuclear Periphery Foci -1.72 -9.70 -5.16 -5.01 0.12 1.08 -5.78 -1.30 -4.82 5.59 0.37 -10.70 -3.40 -6.33 9.76
Nucleolus 1.05 0.32 1.46 0.98 2.17 -0.05 -2.90 2.59 1.55 4.04 -1.41 -4.16 3.02 4.81 6.86
Nucleus 4.12 -10.46 -2.55 -4.82 3.49 13.68 -22.73 -0.70 -12.65 22.29 10.77 -25.02 -1.16 -10.30 23.39
Peroxisomes -0.92 5.22 1.60 2.36 -1.32 -8.03 6.72 -13.70 -4.92 -16.91 -9.18 7.89 -12.87 -6.37 -17.23
Vacuole -10.21 -18.44 -11.86 -10.03 -7.54 0.66 -12.51 -18.91 -18.10 0.46 -4.92 -20.50 -21.88 -18.96 -3.18
Vacuole Periphery -8.20 -5.73 -0.16 2.28 1.69 -7.05 8.12 6.77 10.20 -2.75 -10.06 -2.34 6.01 13.61 6.13
Standard name
Human Ortholog
Description Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes; ATG33 has a paralog, SCM4, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (42%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Atg33

Atg33


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg33-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available