Standard name
Human Ortholog
Description Subunit of the BLOC-1 complex involved in endosomal maturation; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0 0.05 0 0.06 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.56 0.53 0.61 0.61 0.61 0.57 0.56 0.62 0.57 0.6 0.55 0.59 0.66 0.45 0.42 0.54 0.51 0.48 0.22 0.17 0.16 0.19 0.22 0.21
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0.19 0.28 0.28 0.27 0.2 0.08 0.09 0.08 0.11 0.11 0 0.09 0.09 0.32 0.34 0.28 0.24 0.26 0.47 0.47 0.44 0.42 0.36 0.37
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.11 0 0 0.09 0.44 0.41 0.29 0.39 0.35 0.56 0.35 0 0 0 0 0.12 0.08 0.09 0.1 0.16 0.12 0.18 0.13
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0.1 0.17 0.17 0.15 0.07 0.09 0.07 0.05 0.07 0 0.06 0.07 0.28 0.27 0.18 0.22 0.22 0.09 0.07 0.07 0.09 0.07 0.09
Vac/Vac Membrane 0.12 0.14 0 0 0.08 0 0 0 0.08 0.07 0.05 0.08 0 0.07 0.08 0 0 0.09 0.1 0.11 0.07 0.1 0.11 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 0 0 0 1 1 0 1 0 0 0 1 2 1 1 2 0 4 12 2 1 5
Bud 3 4 13 13 22 31 49 29 40 23 51 48 1 0 1 1 3 2 0 3 5 1 1 1
Bud Neck 0 1 3 2 3 0 1 2 3 1 2 0 1 1 5 0 3 2 0 2 3 1 1 2
Bud Site 0 0 0 0 1 1 2 1 1 0 2 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 3 3 2 1 2 2 4 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 116 159 317 317 401 500 585 545 454 500 472 523 45 46 55 48 26 31 60 70 95 44 49 62
Endoplasmic Reticulum 4 0 1 0 1 1 2 1 3 2 4 1 4 6 1 5 2 3 0 4 10 4 2 3
Endosome 40 84 143 141 133 70 97 68 86 88 27 82 6 33 45 25 12 17 131 195 260 96 82 109
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 1 1
Mitochondria 34 33 16 16 61 382 426 258 314 289 484 314 3 0 0 4 6 5 25 42 95 28 40 38
Nucleus 0 5 1 1 4 0 1 13 4 3 3 0 0 0 0 1 0 0 1 0 0 0 0 0
Nuclear Periphery 0 3 0 0 1 1 1 1 1 1 2 0 0 0 1 0 0 0 1 0 4 0 0 0
Nucleolus 0 0 1 1 3 1 1 4 1 2 2 3 1 0 2 1 0 0 0 0 0 0 0 0
Peroxisomes 1 3 31 27 25 33 27 4 14 13 9 6 0 0 1 0 0 0 0 0 2 0 0 0
SpindlePole 15 29 90 87 96 59 93 58 42 57 37 50 5 29 35 16 11 14 24 29 39 19 15 25
Vac/Vac Membrane 24 42 17 10 54 38 37 33 64 56 44 70 3 7 10 4 2 6 27 46 40 22 25 34
Unique Cell Count 206 301 520 517 656 870 1042 882 799 833 865 892 68 103 131 89 51 65 282 413 589 230 229 294
Labelled Cell Count 238 364 634 616 805 1120 1326 1020 1028 1038 1141 1104 69 123 158 106 66 82 282 413 589 230 229 294


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.3 4.1 4.1 4.4 3.6 3.5 4.1 3.9 4.0 3.5 3.8 4.9 4.9 5.2 5.6 5.5 5.3 4.5 4.7 4.9
Std Deviation (1e-4) 0.6 1.1 1.3 1.3 1.9 4.3 1.3 1.2 1.1 1.2 0.9 1.2 1.1 0.6 1.0 0.8 0.8 0.8 0.9 1.0 1.3
Intensity Change (Log2) – – – 0.0 0.11 -0.18 -0.2 0.01 -0.07 -0.02 -0.21 -0.1 0.27 0.26 0.36 0.45 0.44 0.37 0.15 0.2 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.4056 -0.0583 -0.1608 0.0374 -0.0174 0.0419 1.1187 1.2661 1.2727 1.3296 0.8349 1.3839 -0.032 0.4742 0.1801 0.0221 0.5891 -0.256
Actin 0.0142 0.0055 0.0056 0.0002 0.0012 0.0006 0.0226 0.0002 0.0057 0.0049 0.0003 0.0022 0.0728 0.0009 0.0006 0.0193 0.0002 0.0059
Bud 0.0002 0.0007 0.0007 0.0001 0.0043 0.0001 0.0007 0.0001 0.0002 0.0005 0.0001 0.0001 0.0012 0.0001 0.0001 0.0002 0 0.0002
Bud Neck 0.0012 0.0072 0.0021 0.0002 0.0003 0.0004 0.0022 0.0024 0.0022 0.0008 0.0005 0.0008 0.0015 0.0003 0.0003 0.0006 0 0.0006
Bud Periphery 0.0004 0.001 0.001 0.0001 0.0022 0.0001 0.0011 0.0001 0.0007 0.0011 0.0001 0.0002 0.002 0.0001 0.0001 0.0002 0 0.0004
Bud Site 0.0009 0.0034 0.0013 0.0002 0.0011 0.0003 0.0026 0.0021 0.0023 0.0014 0.0005 0.0006 0.0106 0.0005 0.0004 0.0006 0 0.0007
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0.0002 0.0001 0.0006 0.0002 0.0002 0.0002 0.0001 0.0001 0.0015 0.0003 0.0001 0 0 0.0001
Cytoplasm 0.0244 0.0617 0.04 0.0224 0.0436 0.0441 0.0363 0.0404 0.0272 0.0408 0.0083 0.0201 0.0698 0.0663 0.1045 0.0141 0.0055 0.0153
Cytoplasmic Foci 0.2998 0.2854 0.275 0.2794 0.3019 0.2775 0.2554 0.3245 0.2888 0.2919 0.3608 0.3561 0.2541 0.2225 0.2736 0.2192 0.2032 0.2802
Eisosomes 0.0003 0.0001 0.0001 0.0002 0 0.0001 0.0007 0.0001 0.0001 0.0002 0 0 0.001 0.0001 0 0.0001 0 0.0002
Endoplasmic Reticulum 0.0051 0.0017 0.0023 0.0006 0.0017 0.0011 0.0024 0.0012 0.0014 0.0028 0.0008 0.0007 0.0089 0.0043 0.0043 0.0007 0.0005 0.0017
Endosome 0.3098 0.3166 0.3228 0.4529 0.2956 0.3391 0.3022 0.2899 0.3438 0.2913 0.3452 0.3053 0.1959 0.4009 0.2655 0.5051 0.6359 0.2868
Golgi 0.0176 0.0152 0.0204 0.0289 0.02 0.018 0.0233 0.0209 0.0244 0.0314 0.0366 0.0233 0.0299 0.0359 0.0204 0.0674 0.0303 0.0251
Lipid Particles 0.1447 0.0733 0.1233 0.0382 0.0567 0.1452 0.1205 0.1351 0.1027 0.0484 0.0999 0.1126 0.1416 0.1449 0.1356 0.049 0.0724 0.1724
Mitochondria 0.0023 0.0035 0.0036 0.0024 0.0022 0.0042 0.0121 0.0034 0.0065 0.014 0.0368 0.007 0.0058 0.0031 0.0025 0.0285 0.0012 0.0391
None 0.1017 0.1359 0.1145 0.0932 0.1181 0.0912 0.1196 0.0739 0.0833 0.0778 0.0123 0.0559 0.1167 0.0776 0.1038 0.0593 0.026 0.1064
Nuclear Periphery 0.0089 0.0023 0.004 0.0008 0.0025 0.0013 0.0045 0.0008 0.0013 0.0033 0.0013 0.0019 0.0176 0.0014 0.0036 0.001 0.0003 0.006
Nucleolus 0.0012 0.0049 0.0014 0.0007 0.0005 0.0008 0.0024 0.0087 0.0034 0.0012 0.0088 0.0014 0.0008 0.0019 0.0009 0.0001 0.0001 0.0011
Nucleus 0.0113 0.0034 0.0015 0.0007 0.0009 0.0011 0.0026 0.0015 0.0014 0.0018 0.0058 0.0035 0.0234 0.0012 0.0009 0.0001 0 0.0017
Peroxisomes 0.0329 0.0442 0.0578 0.0608 0.1115 0.0478 0.0618 0.0741 0.0804 0.1659 0.0657 0.0911 0.0267 0.015 0.0661 0.0287 0.0174 0.0319
Punctate Nuclear 0.0088 0.0101 0.0072 0.0017 0.0303 0.012 0.012 0.006 0.0119 0.0036 0.0078 0.0089 0.0032 0.0027 0.0034 0.0007 0.0014 0.0065
Vacuole 0.0124 0.0209 0.0131 0.0126 0.0043 0.0126 0.0124 0.013 0.0101 0.0137 0.0068 0.0067 0.0126 0.0178 0.01 0.0034 0.0036 0.0136
Vacuole Periphery 0.0019 0.0027 0.0022 0.0035 0.0011 0.0023 0.002 0.0014 0.0018 0.0031 0.0015 0.0014 0.0024 0.0021 0.003 0.0015 0.0019 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.4854 2.6731 1.9836 7.5221 3.877 5.9772 7.4326 10.033 6.6655 5.5642
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
851 1775 2337 1693 1854 2086 294 1538 2705 3861 2631 3231

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 643.98 743.75 789.20 799.65 654.67 689.16 807.73 801.49 651.31 714.26 791.27 800.53
Standard Deviation 77.99 96.32 94.22 121.91 69.92 91.10 123.02 119.02 72.73 97.42 98.03 120.55
Intensity Change Log 2 — 0.207802 0.293375 0.312353 — 0.074071 0.303105 0.291917 — 0.141935 0.298288 0.302087

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000096 0.000238 0.000367 0.000873 0.000187 0.000487 0.000569 0.001322 0.000158 0.000373 0.000389 0.001087
Bud Neck 0.041552 0.023640 0.090154 0.046484 0.031758 0.030025 0.029573 0.034134 0.034840 0.027090 0.083384 0.040605
Bud Site 0.002243 0.007699 0.023569 0.043622 0.003794 0.011831 0.019919 0.038113 0.003306 0.009931 0.023161 0.041000
Cell Periphery 0.000126 0.000074 0.000056 0.000343 0.000074 0.000180 0.000077 0.000107 0.000090 0.000131 0.000059 0.000231
Cytoplasm 0.156312* 0.090227 0.103016* 0.129428* 0.168580* 0.064881 0.056959 0.123719* 0.164721* 0.076533 0.097870 0.126710*
Cytoplasmic Foci 0.316489* 0.308913* 0.098581 0.105091 0.371534* 0.291117* 0.136984 0.130374 0.354217* 0.299298* 0.102872 0.117126
Eisosomes 0.000195 0.000095 0.000041 0.000101 0.000174 0.000168 0.000075 0.000060 0.000181 0.000134 0.000045 0.000081
Endoplasmic Reticulum 0.000846 0.001112 0.002028 0.001356 0.001198 0.000940 0.002787 0.001012 0.001087 0.001019 0.002113 0.001192
Endosome 0.201507* 0.333628* 0.202279* 0.245931* 0.184487 0.309600* 0.316454* 0.320672* 0.189841 0.320646* 0.215037* 0.281508*
Golgi 0.006885 0.017854 0.002190 0.004119 0.011908 0.048519 0.003201 0.009813 0.010328 0.034421 0.002303 0.006829
Lipid Particles 0.055581 0.037960 0.009747 0.014002 0.055412 0.053007 0.015830 0.010362 0.055466 0.046089 0.010427 0.012269
Mitochondria 0.007702 0.015096 0.010917 0.005462 0.009745 0.047762 0.001938 0.005700 0.009102 0.032745 0.009913 0.005575
Mitotic Spindle 0.002375 0.009504 0.156781* 0.070674 0.002490 0.010329 0.119514* 0.054566 0.002454 0.009950 0.152616* 0.063007
None 0.002385 0.001033 0.001807 0.007288 0.000953 0.002004 0.001336 0.001908 0.001404 0.001557 0.001754 0.004727
Nuclear Periphery 0.001564 0.000807 0.007983 0.001847 0.000964 0.000598 0.002167 0.001086 0.001153 0.000694 0.007333 0.001485
Nuclear Periphery Foci 0.001090 0.001284 0.007127 0.006184 0.000986 0.000931 0.022378 0.003261 0.001018 0.001093 0.008831 0.004792
Nucleolus 0.004584 0.001686 0.026572 0.014432 0.002124 0.001978 0.018484 0.006209 0.002898 0.001844 0.025668 0.010518
Nucleus 0.077598 0.019292 0.107424 0.102068 0.050366 0.012419 0.065434 0.031437 0.058933 0.015579 0.102732 0.068447
Peroxisomes 0.038102 0.033985 0.031093 0.031913 0.037557 0.050777 0.066830 0.045870 0.037729 0.043057 0.035087 0.038556
Vacuole 0.080322 0.091739 0.116741 0.165078 0.063830 0.056251 0.118313 0.174836 0.069019 0.072566 0.116916 0.169723
Vacuole Periphery 0.002447 0.004136 0.001527 0.003707 0.001877 0.006199 0.001177 0.005439 0.002056 0.005250 0.001488 0.004531

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.71 -7.43 -2.73 -2.18 -1.57 -4.39 -7.10 -1.97 -1.25 -0.45 -5.36 -7.51 -3.10 -2.20 -1.89
Bud Neck 4.45 -12.34 -1.08 -8.15 14.15 0.56 -0.95 -0.52 -1.14 0.67 3.55 -17.88 -2.20 -6.66 16.98
Bud Site -5.80 -12.89 -12.91 -10.37 -5.45 -5.81 -6.79 -11.25 -7.15 -2.75 -7.40 -13.02 -16.76 -12.28 -6.20
Cell Periphery 2.75 4.61 -2.69 -3.86 -4.34 -4.33 -2.24 -1.23 3.64 0.57 -2.82 4.72 -3.41 -1.33 -4.61
Cytoplasm 7.92 5.42 2.76 -6.26 -3.09 17.09 9.63 3.10 -12.26 -6.89 17.83 10.27 4.70 -13.39 -5.73
Cytoplasmic Foci 0.97 29.89 26.45 34.61 -4.44 11.60 27.11 38.50 28.51 -1.10 10.07 54.49 48.38 44.74 -7.86
Eisosomes 12.06 19.20 11.54 0.56 -8.07 0.32 6.04 10.10 6.59 5.33 4.00 16.07 12.50 6.17 -7.02
Endoplasmic Reticulum -1.76 -12.70 -4.12 -2.56 7.87 1.34 -6.96 0.54 -0.96 7.21 0.48 -13.55 -2.06 -2.56 12.31
Endosome -11.63 5.26 -0.25 13.41 -6.99 -15.00 -2.30 -7.18 6.17 -1.85 -19.61 5.11 -5.37 13.90 -10.71
Golgi -7.83 5.65 3.68 13.23 -3.70 -16.56 10.94 4.33 18.65 -4.28 -17.35 13.42 6.94 22.34 -6.25
Lipid Particles 4.53 13.19 12.04 11.17 -2.32 0.58 13.25 17.42 16.20 2.64 3.55 21.59 20.80 19.45 -0.53
Mitochondria -5.55 -3.43 3.13 8.95 8.95 -15.48 9.45 5.74 17.85 -2.88 -15.88 -1.24 6.73 19.59 9.51
Mitotic Spindle -4.08 -27.61* -15.89 -14.36 8.67 -6.36 -8.71 -12.86 -10.64 4.64 -7.53 -29.45 -20.51 -17.84 12.61
None 1.79 0.40 -2.47 -3.98 -3.21 -2.63 -3.33 -1.82 0.43 0.71 -0.34 -3.19 -3.74 -3.32 -2.58
Nuclear Periphery 1.65 -15.85 -3.54 -8.75 15.15 1.56 -5.02 -3.88 -7.88 3.67 2.12 -19.52 -6.03 -11.46 17.26
Nuclear Periphery Foci -0.49 -11.28 -6.95 -6.89 1.19 -0.08 -7.42 -5.90 -5.73 6.47 -0.56 -13.78 -9.01 -8.81 5.67
Nucleolus 4.18 -17.47 -7.84 -11.22 8.98 0.40 -6.05 -7.89 -7.80 4.38 3.47 -20.91 -11.63 -13.42 13.01
Nucleus 11.76 -8.27 -2.34 -15.51 6.05 15.00 -4.42 3.46 -10.52 5.42 18.42 -14.68 -2.36 -18.53 12.32
Peroxisomes 1.32 5.00 3.57 3.04 -1.59 -4.79 -1.98 -0.27 4.10 1.81 -2.72 5.65 2.65 5.53 -3.06
Vacuole -2.52 -10.89 -18.06 -17.52 -10.39 2.29 -8.78 -24.37 -25.38 -5.60 -1.48 -18.46 -31.35 -30.74 -14.44
Vacuole Periphery -3.24 2.50 -0.67 2.59 -3.67 -10.87 3.77 -3.19 3.75 -4.63 -10.89 3.96 -3.13 4.82 -5.71
Standard name
Human Ortholog
Description Subunit of the BLOC-1 complex involved in endosomal maturation; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
Localization
Cell Percentages endosome (11%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bls1

Bls1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bls1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available