Standard name
Human Ortholog
Description Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress; human homolog, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0.11 0.07 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.1 0.05 0.08 0.14 0.08 0.09 0 0 0 0 0.06 0.07 0 0 0 0 0 0
Nucleus 0.73 0.76 0.78 0.77 0.75 0.69 0.65 0.65 0.58 0.56 0.6 0.58 0.8 0.79 0.78 0.79 0.79 0.83 0.65 0.69 0.6 0.7 0.65 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.54 0.6 0.5 0.51 0.42 0.56 0.51 0.61 0.62 0.66 0.65 0.64 0.32 0.2 0.2 0.27 0.28 0.28 0.31 0.25 0.35 0.2 0.25 0.38
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 0 0 1 1 2 2 1 5 0 1 0 0 0 0 3 4 1 0 0 5 0 1
Bud Neck 0 1 0 0 0 0 1 5 5 1 3 1 0 0 0 1 1 0 0 0 0 1 0 0
Bud Site 1 1 1 2 2 5 8 20 25 19 16 34 0 0 0 1 5 8 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 9 1 5 5 7 13 20 5 11 6 4 2 19 46 44 29 25 7 0 0 1 4 2 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 1 2 2 0 0 0 0 0 0
Endosome 0 0 2 1 1 0 0 0 0 0 1 0 1 2 0 2 3 4 1 2 1 0 4 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0 2 0
Mitochondria 3 1 5 8 12 31 52 24 38 63 37 43 1 0 0 8 29 27 0 0 2 0 0 1
Nucleus 100 88 273 203 229 242 329 298 284 261 264 263 185 331 300 322 396 313 88 90 220 183 169 188
Nuclear Periphery 1 0 3 1 0 6 3 6 4 4 5 1 0 1 1 5 6 11 0 0 0 0 0 2
Nucleolus 74 70 176 134 128 198 260 278 302 304 287 290 74 85 76 111 142 104 41 32 130 53 64 131
Peroxisomes 0 0 1 0 1 0 2 2 0 0 0 0 0 0 1 1 1 1 0 0 0 0 0 0
SpindlePole 1 1 0 0 1 0 2 1 2 0 3 0 3 12 6 4 2 1 0 0 1 3 5 6
Vac/Vac Membrane 1 2 4 0 0 3 0 0 1 1 2 1 0 8 7 7 9 5 2 3 6 6 8 11
Unique Cell Count 137 116 349 262 305 352 507 457 487 463 442 456 231 418 383 408 503 378 136 130 371 263 262 351
Labelled Cell Count 190 165 470 354 383 499 679 641 674 665 622 636 283 485 435 492 626 487 136 130 371 263 262 351


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.9 11.9 10.7 9.2 9.9 8.0 8.5 8.7 8.0 8.0 7.9 7.7 12.4 12.7 12.2 13.8 13.3 12.5 12.3 13.6 15.3
Std Deviation (1e-4) 4.2 3.7 3.3 2.9 3.0 2.2 3.0 2.7 2.7 2.7 2.5 2.5 4.7 4.4 4.2 5.6 5.6 5.4 4.0 3.7 3.8
Intensity Change (Log2) – – – -0.22 -0.12 -0.42 -0.34 -0.31 -0.43 -0.43 -0.45 -0.47 0.21 0.24 0.18 0.36 0.31 0.22 0.2 0.34 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 3.6 4.0 3.5 3.2 4.9 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.8 1.9 2.1 0 0.9 0 0 0 4.0 5.3 5.4 3.8 2.8 0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.4 2.0 4.4 5.1 2.9 4.1 6.2 4.3 4.7 0 0 0 0.6 3.2 3.7
Nucleus -0.2 -0.9 -2.8 -4.2 -4.0 -6.0 -6.5 -5.5 -6.1 0.5 0.3 0 0.2 0.2 1.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.0
Nucleolus 0.2 -2.2 1.5 0.2 3.0 3.3 4.4 4.1 3.7 -4.4 -8.8 -8.7 -6.6 -6.6 -6.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0.9 0.8 0.7 0.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.4897 9.0921 9.4992 8.6371 8.5349 8.5387 11.726 9.891 9.8414 8.8935 9.9418 9.3332 10.124 8.8187 8.8587 8.5096 8.3057 8.2311
Actin 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0
Bud Neck 0 0 0 0 0.0003 0.0005 0 0 0 0 0.0001 0.0001 0 0 0 0 0.0001 0.0001
Bud Periphery 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0002 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0
Cytoplasm 0 0 0 0 0 0 0.0028 0 0 0 0 0 0 0 0 0 0 0
Cytoplasmic Foci 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.0004 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Lipid Particles 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.0001 0 0 0 0.0019 0 0.0001 0 0 0 0.0001 0 0.0003 0 0 0 0.0028 0
None 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.0005 0.0004 0.0001 0.0016 0.0001 0.0007 0.0003 0.0001 0.0001 0.0001 0 0.0001 0.0004 0.0001 0 0.0008 0
Nucleolus 0.0735 0.0534 0.0504 0.0654 0.3602 0.2544 0.0506 0.0567 0.0325 0.049 0.2535 0.1758 0.0787 0.0572 0.0597 0.0477 0.3593 0.1816
Nucleus 0.9263 0.9447 0.9489 0.9341 0.6285 0.7434 0.945 0.9412 0.9671 0.9503 0.7447 0.8238 0.9207 0.9421 0.9401 0.9512 0.6353 0.8181
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Punctate Nuclear 0.0001 0.0013 0.0002 0.0004 0.0067 0.0014 0.0001 0.0016 0.0002 0.0004 0.0014 0.0001 0 0.0003 0.0001 0.0009 0.0008 0.0001
Vacuole 0 0 0 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0.0002 0
Vacuole Periphery 0 0 0 0 0.0003 0 0 0 0 0 0 0 0 0 0 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.9374 22.5314 18.577 29.6471 33.2841 18.6598 25.7347 31.0367 26.1593 29.3312
Translational Efficiency 2.6361 2.5088 3.1357 1.7059 1.8557 3.1328 2.3115 1.8625 2.1264 2.0533

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
718 130 1638 1391 1263 1179 135 648 1981 1309 1773 2039

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1048.29 1246.77 1336.27 1521.85 1081.16 1107.21 1403.72 1579.44 1069.25 1121.07 1341.41 1540.15
Standard Deviation 236.22 244.32 277.79 365.14 215.85 237.46 292.39 371.89 224.01 241.78 279.50 368.28
Intensity Change Log 2 — 0.250157 0.350174 0.537788 — 0.034349 0.376675 0.546833 — 0.144621 0.363690 0.542388

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000588 0.000685 0.000841 0.001047 0.000665 0.001152 0.001288 0.000858 0.000637 0.001105 0.000875 0.000987
Bud Neck 0.003722 0.006029 0.012123 0.010224 0.005857 0.006140 0.008282 0.010717 0.005083 0.006129 0.011831 0.010380
Bud Site 0.000449 0.000565 0.001144 0.002785 0.000582 0.000803 0.001713 0.002063 0.000534 0.000779 0.001187 0.002556
Cell Periphery 0.000043 0.000020 0.000042 0.000217 0.000036 0.000055 0.000081 0.000134 0.000038 0.000051 0.000045 0.000191
Cytoplasm 0.000667 0.000030 0.000220 0.000872 0.000072 0.000599 0.000525 0.001296 0.000288 0.000542 0.000243 0.001007
Cytoplasmic Foci 0.000090 0.000064 0.000093 0.000709 0.000077 0.000610 0.000231 0.001266 0.000082 0.000556 0.000104 0.000886
Eisosomes 0.000071 0.000073 0.000123 0.000095 0.000089 0.000086 0.000118 0.000075 0.000082 0.000085 0.000122 0.000088
Endoplasmic Reticulum 0.000790 0.000625 0.000589 0.000451 0.000959 0.001068 0.001672 0.000539 0.000897 0.001024 0.000672 0.000479
Endosome 0.000046 0.000027 0.000028 0.000681 0.000057 0.000216 0.000068 0.002858 0.000053 0.000197 0.000031 0.001373
Golgi 0.000102 0.000091 0.000102 0.001279 0.000101 0.000566 0.000155 0.001244 0.000101 0.000519 0.000106 0.001268
Lipid Particles 0.000028 0.000011 0.000011 0.000137 0.000068 0.000687 0.000052 0.000228 0.000053 0.000620 0.000014 0.000166
Mitochondria 0.000999 0.001175 0.001792 0.003538 0.001170 0.001475 0.002734 0.003998 0.001108 0.001445 0.001864 0.003684
Mitotic Spindle 0.005866 0.000347 0.002172 0.004869 0.002006 0.007904 0.004500 0.006265 0.003405 0.007154 0.002349 0.005313
None 0.002241 0.000621 0.001268 0.001384 0.000871 0.003558 0.007657 0.001400 0.001368 0.003266 0.001754 0.001389
Nuclear Periphery 0.000077 0.000072 0.000110 0.000409 0.000117 0.000101 0.000137 0.000512 0.000102 0.000098 0.000112 0.000442
Nuclear Periphery Foci 0.000165 0.000030 0.000038 0.000370 0.000170 0.000249 0.000091 0.000547 0.000168 0.000227 0.000042 0.000426
Nucleolus 0.172077 0.212171 0.131982 0.102493 0.175024 0.173863 0.098099 0.092550 0.173956 0.177668 0.129403 0.099333
Nucleus 0.806380* 0.771029* 0.840856* 0.861208* 0.808201* 0.794598* 0.862913* 0.865558* 0.807541* 0.792257* 0.842535* 0.862591*
Peroxisomes 0.005079 0.005939 0.006011 0.004589 0.003355 0.005802 0.008666 0.004577 0.003980 0.005816 0.006213 0.004585
Vacuole 0.000264 0.000156 0.000199 0.001303 0.000191 0.000232 0.000669 0.001933 0.000218 0.000225 0.000235 0.001503
Vacuole Periphery 0.000258 0.000241 0.000256 0.001340 0.000333 0.000238 0.000349 0.001383 0.000306 0.000238 0.000263 0.001354

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.58 -9.25 -4.89 -3.44 -1.62 -1.68 -5.99 -2.84 0.88 3.51 -1.80 -10.06 -5.20 0.35 -0.93
Bud Neck -2.09 -21.16 -7.11 -3.23 1.83 -1.18 -2.97 -4.03 -3.62 -0.86 -3.24 -18.33 -7.31 -5.50 1.70
Bud Site -0.94 -9.66 -3.51 -3.32 -2.31 -2.06 -3.10 -2.82 -2.12 -0.23 -2.41 -10.55 -4.20 -3.35 -2.61
Cell Periphery 1.77 -0.12 -3.09 -3.46 -3.11 -1.81 -2.13 -2.39 -1.90 -1.09 -1.39 -1.33 -3.71 -3.38 -3.55
Cytoplasm 1.26 0.90 -0.40 -3.23 -2.47 -2.34 -2.11 -1.36 -0.55 -0.84 -1.21 0.31 -1.90 -0.80 -2.31
Cytoplasmic Foci 0.84 -0.90 -3.78 -3.91 -3.67 -2.00 -1.79 -3.12 -1.09 -2.41 -1.99 -1.72 -4.89 -0.84 -4.66
Eisosomes -0.35 -11.66 -4.54 -2.53 5.33 0.41 -3.79 1.92 1.70 4.78 -0.78 -10.44 -1.72 -0.97 7.71
Endoplasmic Reticulum 1.25 1.70 3.46 1.39 2.73 -1.44 -2.75 2.62 3.24 3.87 -2.01 3.23 5.45 6.87 3.20
Endosome 1.67 1.51 -2.89 -2.98 -2.96 -2.03 -0.72 -2.22 -2.05 -2.21 -2.05 2.47 -3.12 -2.63 -3.17
Golgi 0.71 -1.31 -1.66 -1.68 -1.64 -2.07 -3.24 -2.15 -0.99 -1.98 -2.07 -2.27 -2.26 -1.20 -2.22
Lipid Particles 1.61 1.29 -1.39 -1.64 -1.59 -1.80 0.32 -1.03 1.33 -1.24 -1.83 1.09 -1.41 1.50 -2.15
Mitochondria -1.50 -12.16 -6.28 -5.70 -4.14 -2.02 -3.08 -3.07 -2.69 -1.02 -2.55 -10.87 -6.49 -5.42 -4.32
Mitotic Spindle 2.17 1.04 0.33 -3.44 -1.16 -2.79 -0.88 -1.96 0.63 -0.46 -1.95 0.16 -1.32 1.03 -1.59
None 2.54 1.43 1.22 -4.04 -0.55 -2.47 -2.36 -2.01 1.90 2.13 -1.88 -0.93 -0.25 1.84 0.86
Nuclear Periphery 0.07 -2.19 -3.19 -3.09 -2.75 0.31 -0.75 -2.52 -2.68 -2.30 0.07 -0.88 -3.83 -3.97 -3.65
Nuclear Periphery Foci 2.65 2.28 -0.97 -1.69 -1.59 -1.11 1.57 -1.36 -0.95 -1.77 -1.07 3.27 -1.49 -0.92 -2.33
Nucleolus -2.19 4.55 8.62 6.10 5.20 0.15 5.15 10.20 10.03 0.73 -0.65 7.11 13.89 13.09 5.92
Nucleus 1.88 -3.25 -5.73 -4.74 -3.31 2.10 -2.89 -5.43 -6.80 -0.51 2.57 -4.64 -8.39 -9.74 -3.66
Peroxisomes -0.82 -2.09 0.85 1.30 3.55 -4.52 -4.50 -2.28 1.61 3.23 -3.70 -5.76 -2.00 2.39 4.12
Vacuole 0.94 0.25 -2.99 -3.42 -3.21 -1.24 -2.87 -2.65 -2.28 -1.65 -0.95 -1.20 -4.13 -3.49 -3.98
Vacuole Periphery 0.54 -0.16 -2.46 -2.50 -2.45 1.93 -0.34 -2.59 -2.84 -2.30 1.91 0.99 -3.17 -3.36 -3.28
Standard name
Human Ortholog
Description Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress; human homolog, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Cdc73

Cdc73


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc73-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available