Standard name
Human Ortholog
Description Dihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.95 0.88 0.88 0.75 0.76 0.74 0.73 0.75 0.73 0.98 0.99 0.98 0.93 0.95 0.93 0.94 0.95 0.89 0.96 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.09 0.05 0.13 0.12 0.23 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0.09 0.21 0.18 0.18 0.2 0.1 0.17 0 0 0 0.07 0.05 0.08 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1
Bud 1 0 2 0 0 1 0 4 15 3 12 8 0 0 0 1 0 0 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 2 1 6 3 12 13 11 8 6 7 2 2 2 33 9 12 0 0 0 0 0 0
Cytoplasm 215 200 195 305 375 267 335 423 366 384 309 293 343 432 422 369 208 380 202 198 183 211 449 399
Endoplasmic Reticulum 1 2 0 1 6 4 3 10 7 3 1 8 3 0 1 7 4 10 1 1 0 1 2 6
Endosome 0 0 0 0 1 0 0 0 0 1 1 1 0 0 0 1 0 1 1 0 0 0 0 2
Golgi 1 0 1 1 2 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 1 0
Mitochondria 1 0 0 0 2 7 39 28 64 63 93 51 0 0 1 1 0 2 2 0 3 0 0 0
Nucleus 3 1 2 4 4 4 5 11 10 12 6 11 5 2 7 5 1 0 0 0 2 0 5 1
Nuclear Periphery 0 1 0 0 0 0 1 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 2 1 1 0 2 0 0 1 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 4 12 43 26 95 101 88 104 43 67 3 7 6 26 11 34 1 3 11 4 7 5
Unique Cell Count 218 204 201 321 424 303 448 556 497 528 412 404 351 437 430 397 220 407 215 208 206 220 477 427
Labelled Cell Count 223 206 206 325 439 312 493 591 562 579 474 448 357 444 440 444 234 439 215 208 206 220 477 427


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 16.8 13.6 13.4 12.0 9.6 9.0 8.9 7.8 7.7 7.1 7.1 18.5 18.3 17.5 16.1 17.2 16.9 14.8 15.2 15.4
Std Deviation (1e-4) 2.9 3.6 2.5 2.7 2.3 2.3 2.3 2.1 2.2 2.1 2.1 2.0 3.9 3.8 4.0 3.7 3.3 3.7 3.1 3.3 3.4
Intensity Change (Log2) – – – -0.02 -0.17 -0.49 -0.6 -0.61 -0.81 -0.82 -0.93 -0.92 0.45 0.43 0.37 0.25 0.34 0.32 0.13 0.16 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 3.6 2.0 0
Cytoplasm -1.1 -3.5 -3.5 -6.8 -6.6 -7.0 -7.2 -6.7 -7.2 0.5 1.7 0.9 -2.0 -1.3 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.3 3.2 5.3 5.1 7.3 5.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.1 3.6 3.1 6.3 5.7 5.6 6.0 3.7 5.3 0 0 0 2.4 1.7 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.5488 12.8539 11.7539 11.4504 11.1327 11.1059 9.0689 11.4092 10.1314 9.2908 9.0848 10.6281 8.2312 12.1156 10.4939 9.2341 10.0117 10.7112
Actin 0.0046 0.0002 0.0015 0.0002 0.0003 0.0008 0.0101 0.0002 0.0043 0.0009 0.0076 0.0009 0.0522 0.0003 0.0211 0.0567 0.0001 0.0001
Bud 0.0003 0.0002 0.0002 0.0001 0.0003 0.0006 0.0003 0.0001 0.0002 0.0007 0.0012 0.0016 0.0011 0 0.0008 0.0054 0.0001 0.0002
Bud Neck 0.0008 0.0002 0.0004 0.0005 0.0006 0.0012 0.0011 0.0001 0.0005 0.0009 0.0013 0.001 0.0014 0.0001 0.0006 0.0049 0.0002 0.0005
Bud Periphery 0.0002 0 0.0001 0 0.0001 0.0006 0.0003 0 0.0001 0.0006 0.0011 0.0002 0.0009 0 0.0004 0.011 0 0.0002
Bud Site 0.0003 0.0003 0.0003 0.0001 0.0001 0.0002 0.0021 0.0001 0.0004 0.0006 0.0012 0 0.011 0.0001 0.0014 0.0075 0 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0003 0 0 0.0001 0.0004 0 0.0004 0 0.0001 0.0005 0 0.0001
Cytoplasm 0.9151 0.9798 0.9385 0.9524 0.9454 0.9414 0.8954 0.9924 0.924 0.9061 0.8648 0.9738 0.7846 0.9931 0.8951 0.637 0.9929 0.9364
Cytoplasmic Foci 0.006 0.0018 0.0039 0.0013 0.0041 0.0042 0.0183 0.0009 0.0136 0.0111 0.0104 0.0023 0.0374 0.0008 0.0081 0.0231 0.0007 0.0013
Eisosomes 0 0 0 0 0 0 0.0019 0 0 0 0.0001 0 0.0001 0 0 0.0003 0 0
Endoplasmic Reticulum 0.0058 0.0009 0.003 0.0014 0.0028 0.0025 0.0026 0.0004 0.0027 0.0024 0.0032 0.0015 0.0041 0.0009 0.0069 0.0025 0.0006 0.0014
Endosome 0.0168 0.0013 0.0036 0.0008 0.0032 0.0038 0.0155 0.0003 0.0142 0.0441 0.0185 0.0013 0.0364 0.0005 0.0218 0.1375 0.0006 0.0177
Golgi 0.0013 0.0001 0.0007 0 0.0003 0.0002 0.0042 0 0.0045 0.003 0.0032 0.0001 0.01 0.0001 0.0049 0.0353 0 0.0005
Lipid Particles 0.0007 0 0.0031 0 0 0 0.0077 0 0.007 0.0004 0.0006 0 0.0218 0 0.001 0.0103 0 0.0001
Mitochondria 0.0013 0.0001 0.0009 0.0001 0.0016 0.0006 0.005 0 0.0013 0.0022 0.0334 0.0002 0.0021 0 0.0019 0.0172 0 0.0015
None 0.0065 0.0012 0.0025 0.0014 0.0043 0.0019 0.0077 0.0007 0.0026 0.0014 0.0015 0.0028 0.0059 0.0011 0.0243 0.0006 0.0004 0.0005
Nuclear Periphery 0.008 0.0014 0.0041 0.0043 0.0035 0.0037 0.0035 0.0005 0.0052 0.0027 0.0038 0.0016 0.0029 0.0004 0.0028 0.0026 0.0011 0.0034
Nucleolus 0.0001 0 0.0001 0 0 0.0001 0.0006 0 0.0001 0.0001 0.0085 0 0.0003 0 0.0001 0.0003 0 0.0001
Nucleus 0.0254 0.0114 0.0329 0.0356 0.0304 0.0338 0.0137 0.004 0.0127 0.0131 0.0155 0.0112 0.0064 0.0023 0.0051 0.0036 0.0023 0.0165
Peroxisomes 0.0004 0 0.0009 0 0.0001 0.0001 0.0042 0 0.0041 0.0002 0.0021 0.0002 0.0101 0 0.0004 0.0118 0 0
Punctate Nuclear 0.0018 0.0004 0.0021 0.0009 0.0014 0.0013 0.003 0.0001 0.0013 0.0012 0.0007 0.0003 0.0052 0.0001 0.0011 0.0007 0.0001 0.0002
Vacuole 0.0034 0.0006 0.0009 0.0006 0.0012 0.0023 0.0019 0.0001 0.0008 0.0062 0.0164 0.0007 0.005 0.0001 0.0014 0.0261 0.0006 0.0122
Vacuole Periphery 0.0011 0.0001 0.0002 0.0001 0.0003 0.0004 0.0006 0 0.0003 0.0019 0.0045 0.0002 0.0009 0 0.0006 0.0052 0.0002 0.007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.591 61.2544 52.8062 56.7628 46.3806 49.3488 50.0939 50.1003 52.3039 59.1446
Translational Efficiency 1.3146 1.3191 1.5169 1.2647 1.521 1.6804 1.5943 1.3327 1.3328 1.348

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
77 740 2355 1424 1219 1747 203 1071 1296 2487 2558 2495

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 986.18 1595.72 1447.31 1464.27 1198.47 1488.26 1507.29 1390.26 1185.86 1520.23 1452.07 1432.50
Standard Deviation 252.12 301.02 284.38 377.53 225.65 266.33 322.54 376.36 232.79 281.43 288.05 378.81
Intensity Change Log 2 — 0.694285 0.553451 0.570259 — 0.312433 0.330763 0.214161 — 0.497391 0.435561 0.385851

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001092 0.000349 0.000138 0.000616 0.000199 0.000555 0.000840 0.000385 0.000252 0.000493 0.000194 0.000517
Bud Neck 0.008683 0.001115 0.001653 0.003244 0.000420 0.000816 0.003561 0.003905 0.000911 0.000905 0.001805 0.003527
Bud Site 0.040021 0.007023 0.004220 0.015816 0.002432 0.007120 0.009079 0.024468 0.004666 0.007091 0.004605 0.019530
Cell Periphery 0.000415 0.000141 0.000060 0.000154 0.000079 0.000071 0.001163 0.000142 0.000099 0.000092 0.000147 0.000149
Cytoplasm 0.522819* 0.859024* 0.778925* 0.694380* 0.864049* 0.871921* 0.563332* 0.737020* 0.843776* 0.868084* 0.761815* 0.712684*
Cytoplasmic Foci 0.165975 0.026379 0.005689 0.011232 0.024586 0.023498 0.020027 0.012405 0.032986 0.024355 0.006827 0.011735
Eisosomes 0.000072 0.000004 0.000002 0.000011 0.000004 0.000004 0.000040 0.000009 0.000008 0.000004 0.000005 0.000010
Endoplasmic Reticulum 0.006606 0.000406 0.000172 0.000985 0.002616 0.000779 0.002086 0.000521 0.002853 0.000668 0.000323 0.000786
Endosome 0.021970 0.001516 0.000339 0.004084 0.001283 0.002464 0.005466 0.006351 0.002512 0.002182 0.000746 0.005057
Golgi 0.004154 0.001689 0.000168 0.006695 0.001095 0.003480 0.000541 0.003207 0.001277 0.002947 0.000197 0.005198
Lipid Particles 0.004968 0.000713 0.000367 0.000917 0.000481 0.000573 0.003146 0.000628 0.000748 0.000614 0.000588 0.000793
Mitochondria 0.001876 0.000126 0.000291 0.003684 0.000218 0.000601 0.000192 0.003356 0.000316 0.000460 0.000283 0.003543
Mitotic Spindle 0.004147 0.008753 0.004334 0.028680 0.003501 0.007750 0.005060 0.026076 0.003539 0.008049 0.004392 0.027562
None 0.002047 0.004164 0.008767 0.007895 0.007532 0.005121 0.003423 0.009684 0.007206 0.004836 0.008343 0.008663
Nuclear Periphery 0.000641 0.000100 0.000209 0.000862 0.000198 0.000140 0.000902 0.000356 0.000224 0.000128 0.000264 0.000645
Nuclear Periphery Foci 0.011143 0.004765 0.000976 0.005137 0.005197 0.002714 0.012539 0.002505 0.005551 0.003324 0.001894 0.004007
Nucleolus 0.003929 0.000103 0.000143 0.000739 0.000112 0.000097 0.000165 0.000187 0.000339 0.000099 0.000145 0.000502
Nucleus 0.097541 0.064497 0.176848* 0.159770* 0.064920 0.055158 0.144405* 0.122019 0.066858 0.057937 0.174273* 0.143565*
Peroxisomes 0.004025 0.000115 0.000103 0.000494 0.000080 0.000204 0.000235 0.000456 0.000314 0.000178 0.000113 0.000478
Vacuole 0.090376 0.018250 0.016473 0.045688 0.019724 0.015810 0.223559* 0.043829 0.023922 0.016536 0.032907 0.044890
Vacuole Periphery 0.007501 0.000768 0.000123 0.008917 0.001273 0.001122 0.000238 0.002492 0.001643 0.001017 0.000132 0.006159

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.08 2.96 2.07 0.04 -3.45 -2.89 -6.90 -4.78 1.38 5.58 -3.02 -1.05 -3.40 1.06 -3.02
Bud Neck 3.25 3.04 2.88 -3.02 -1.18 -3.20 -10.94 -6.72 -4.87 4.60 -0.61 -4.06 -6.52 -6.53 -0.93
Bud Site 3.36 3.66 3.32 -0.30 -2.67 -4.90 -9.14 -5.85 -3.21 -0.95 -2.44 -0.76 -4.77 -2.73 -4.27
Cell Periphery 4.04 6.18 4.80 1.95 -7.55 0.41 -3.36 -7.50 -8.51 2.66 -1.19 -1.90 -6.71 -6.56 -2.88
Cytoplasm -8.48 -5.84 -3.32 21.51 12.78 -0.54 20.76 23.72 24.89 -10.09 -2.14 19.62 27.40 35.76 9.80
Cytoplasmic Foci 6.42 7.62 7.55 9.74 -1.73 0.56 1.65 5.20 5.19 2.83 2.89 10.98 9.63 11.14 -3.96
Eisosomes 6.48 6.62 5.94 -6.33 -9.10 0.18 -10.39 -9.20 -9.67 7.93 4.10 1.70 -3.01 -11.29 -7.34
Endoplasmic Reticulum 3.60 3.70 3.55 -0.90 -3.56 2.26 0.54 2.68 2.15 7.14 2.83 3.20 3.08 1.65 -1.50
Endosome 3.59 3.78 3.63 0.42 -3.33 -2.26 -5.88 -1.34 0.32 3.15 0.98 4.35 1.88 1.06 -3.11
Golgi 2.11 3.36 1.95 -0.08 -2.14 -2.36 1.77 0.32 2.52 -1.24 -2.05 3.31 0.02 1.97 -2.75
Lipid Particles 2.92 3.21 2.73 -1.69 -5.13 -1.24 -10.32 -8.53 -7.15 6.73 -0.33 -2.88 -6.74 -7.74 -3.97
Mitochondria 1.88 1.69 0.86 -1.89 -1.50 -1.74 -0.58 -1.17 -0.61 -1.10 -0.73 0.25 -1.70 -1.37 -1.80
Mitotic Spindle -1.00 -0.64 -4.38 -3.66 -5.45 -1.99 -2.21 -4.94 -3.43 -2.59 -2.29 -1.97 -7.14 -5.29 -6.66
None -2.54 -9.88 -7.10 -5.11 3.46 2.27 3.35 0.61 -3.22 -5.12 2.47 -0.34 0.54 -5.03 2.67
Nuclear Periphery 2.36 1.18 -0.47 -11.28 -6.52 0.81 -10.97 -6.77 -7.12 8.38 1.50 -5.99 -8.47 -11.22 -4.69
Nuclear Periphery Foci 1.11 1.84 1.64 4.39 -2.23 3.34 -4.54 3.16 -0.30 6.90 2.50 4.94 3.76 2.61 -1.81
Nucleolus 2.53 2.49 2.39 -2.62 -1.99 0.71 -4.01 -1.48 -2.90 3.23 2.54 1.88 0.96 -3.15 -1.74
Nucleus 1.52 -4.09 -2.97 -19.75 4.85 2.71 -8.62 -15.92 -18.93 1.61 2.09 -30.66 -23.36 -29.95 8.28
Peroxisomes 3.75 3.77 3.71 -0.73 -1.08 -2.77 -5.63 -2.72 -0.28 0.86 2.03 2.87 1.85 -0.21 -1.71
Vacuole 3.24 2.96 -1.70 -16.76 -17.37 -0.04 -14.99 -18.09 -18.41 8.75 0.20 -10.28 -24.42 -26.60 -15.24
Vacuole Periphery 2.05 2.21 1.61 -1.77 -2.59 0.43 2.11 1.00 0.85 -1.59 1.33 3.00 0.30 -1.09 -3.03
Standard name
Human Ortholog
Description Dihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ura4

Ura4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ura4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available