Standard name | |
---|---|
Human Ortholog | |
Description | Component of the Dsl1p tethering complex; this complex interacts with ER SNAREs Sec20p and Use1p; mediates Sey1p-independent homotypic ER fusion; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 46 | 23 | 49 | 30 | 25 | 10 | 15 | 15 | 5 | 5 | 4 | 13 | 31 | 9 | 6 | 12 | 5 | 2 | 18 | 10 | 24 | 6 | 21 | 10 |
Bud | 14 | 9 | 13 | 9 | 3 | 9 | 13 | 7 | 23 | 20 | 13 | 22 | 7 | 3 | 1 | 2 | 2 | 1 | 4 | 1 | 3 | 1 | 1 | 0 |
Bud Neck | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 6 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 14 | 12 | 52 | 26 | 42 | 22 | 13 | 10 | 12 | 8 | 5 | 12 | 19 | 20 | 24 | 21 | 14 | 7 | 5 | 6 | 17 | 1 | 16 | 15 |
Cytoplasm | 68 | 74 | 74 | 115 | 161 | 213 | 262 | 198 | 174 | 175 | 139 | 204 | 57 | 77 | 129 | 9 | 10 | 5 | 14 | 28 | 22 | 3 | 7 | 3 |
Endoplasmic Reticulum | 255 | 194 | 193 | 108 | 100 | 101 | 149 | 93 | 27 | 39 | 22 | 68 | 159 | 130 | 113 | 3 | 2 | 1 | 329 | 250 | 342 | 78 | 174 | 153 |
Endosome | 0 | 1 | 0 | 3 | 3 | 3 | 4 | 2 | 0 | 0 | 0 | 4 | 0 | 0 | 10 | 0 | 0 | 0 | 3 | 1 | 4 | 1 | 2 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 4 | 3 | 4 | 0 | 2 | 3 |
Mitochondria | 24 | 13 | 6 | 33 | 23 | 101 | 126 | 53 | 204 | 158 | 166 | 110 | 13 | 3 | 4 | 2 | 1 | 3 | 20 | 25 | 81 | 4 | 26 | 19 |
Nucleus | 0 | 0 | 5 | 1 | 4 | 5 | 9 | 19 | 1 | 7 | 3 | 7 | 1 | 1 | 0 | 2 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 3 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 14 | 8 | 18 | 17 | 31 | 47 | 46 | 69 | 45 | 33 | 42 | 58 | 4 | 1 | 18 | 8 | 9 | 6 | 20 | 21 | 25 | 7 | 9 | 18 |
Unique Cell Count | 372 | 300 | 349 | 289 | 342 | 436 | 555 | 421 | 406 | 370 | 331 | 434 | 247 | 218 | 264 | 50 | 41 | 24 | 432 | 362 | 541 | 106 | 274 | 240 |
Labelled Cell Count | 439 | 335 | 411 | 343 | 394 | 512 | 641 | 473 | 501 | 445 | 397 | 502 | 292 | 245 | 307 | 62 | 48 | 25 | 432 | 362 | 541 | 106 | 274 | 240 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 7.2 | 7.1 | 6.4 | 6.5 | 5.3 | 5.3 | 5.6 | 4.6 | 4.6 | 4.4 | 5.0 | 7.4 | 7.6 | 7.9 | 10.2 | 10.1 | 10.3 | 7.2 | 7.2 | 8.0 |
Std Deviation (1e-4) | 1.0 | 1.0 | 0.7 | 0.9 | 1.1 | 1.1 | 0.8 | 0.9 | 0.8 | 0.9 | 0.9 | 0.7 | 1.1 | 1.0 | 1.2 | 1.4 | 1.4 | 1.1 | 1.2 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.12 | -0.42 | -0.41 | -0.33 | -0.63 | -0.64 | -0.68 | -0.49 | 0.07 | 0.1 | 0.16 | 0.53 | 0.51 | 0.54 | 0.02 | 0.01 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.4 | -2.9 | -6.2 | -6.5 | -5.2 | 0 | 0 | 0 | -5.7 | -0.5 | -3.8 | -5.0 | 1.8 | -0.3 | 0 |
Bud | -0.4 | -2.5 | -1.4 | -1.2 | -1.8 | 0 | 0 | 0 | 0.9 | -0.6 | -1.6 | -2.7 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.3 | -1.0 | -4.7 | -7.1 | -6.4 | 0 | 0 | 0 | -6.2 | -2.7 | -2.0 | -2.2 | 4.6 | 3.1 | 0 |
Cytoplasm | 5.1 | 7.2 | 8.0 | 7.9 | 7.5 | 6.3 | 7.3 | 5.8 | 7.5 | 0.5 | 3.7 | 7.2 | -0.5 | 0.5 | 0 |
Endoplasmic Reticulum | -4.5 | -6.9 | -9.2 | -8.6 | -9.5 | 0 | 0 | 0 | -11.7 | 2.2 | 1.0 | -3.1 | -6.5 | -6.1 | -4.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.1 | 3.3 | 8.7 | 8.7 | 5.6 | 0 | 0 | 0 | 9.3 | 2.4 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0.2 | 2.4 | 0 | 0.5 | 0 | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.4 | 2.0 | 2.8 | 1.8 | 4.9 | 2.9 | 2.0 | 3.5 | 3.9 | -2.3 | -3.0 | 0.9 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1367 | 3.029 | 2.7431 | 2.4045 | 2.0018 | 2.6652 | 3.9377 | 4.3485 | 4.13 | 3.7611 | 3.4185 | 3.9505 | 3.5433 | 3.5361 | 3.3499 | 3.6721 | 3.0656 | 3.5917 |
Actin | 0.0487 | 0.0084 | 0.0197 | 0.0142 | 0.0158 | 0.0152 | 0.0352 | 0.0045 | 0.0158 | 0.0054 | 0.0076 | 0.0033 | 0.0115 | 0.0065 | 0.0119 | 0.0023 | 0.0114 | 0.0034 |
Bud | 0.001 | 0.0014 | 0.007 | 0.0028 | 0.0004 | 0.0005 | 0.0006 | 0.0005 | 0.0013 | 0.0003 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0033 | 0.0003 | 0.0003 | 0.0001 |
Bud Neck | 0.0029 | 0.0016 | 0.0026 | 0.0004 | 0.0013 | 0.0258 | 0.0014 | 0.0007 | 0.0018 | 0.0008 | 0.0032 | 0.0065 | 0.0033 | 0.001 | 0.0007 | 0.0005 | 0.0007 | 0.007 |
Bud Periphery | 0.0015 | 0.0014 | 0.0108 | 0.0079 | 0.0008 | 0.001 | 0.0009 | 0.0004 | 0.0034 | 0.0006 | 0.0007 | 0.0003 | 0.0005 | 0.0004 | 0.0078 | 0.0006 | 0.0005 | 0.0003 |
Bud Site | 0.0185 | 0.0179 | 0.0237 | 0.0004 | 0.0014 | 0.0009 | 0.0079 | 0.02 | 0.0108 | 0.001 | 0.001 | 0.0001 | 0.0106 | 0.0176 | 0.0016 | 0.0002 | 0.0009 | 0.0002 |
Cell Periphery | 0.0028 | 0.0048 | 0.0045 | 0.0007 | 0.0007 | 0.0014 | 0.002 | 0.0035 | 0.0055 | 0.0009 | 0.0012 | 0.0008 | 0.0011 | 0.0035 | 0.0015 | 0.0016 | 0.0004 | 0.0007 |
Cytoplasm | 0.2587 | 0.3937 | 0.3227 | 0.2394 | 0.1587 | 0.3751 | 0.1034 | 0.2278 | 0.2089 | 0.1104 | 0.1955 | 0.1757 | 0.1855 | 0.3362 | 0.2117 | 0.2893 | 0.2633 | 0.2664 |
Cytoplasmic Foci | 0.0247 | 0.019 | 0.0262 | 0.0111 | 0.0124 | 0.0249 | 0.0225 | 0.015 | 0.0132 | 0.0094 | 0.0151 | 0.0104 | 0.0221 | 0.0125 | 0.0238 | 0.0039 | 0.0168 | 0.0141 |
Eisosomes | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.4095 | 0.4188 | 0.3879 | 0.5777 | 0.5326 | 0.3966 | 0.6377 | 0.6255 | 0.6192 | 0.7722 | 0.6554 | 0.7019 | 0.6151 | 0.5418 | 0.6234 | 0.6582 | 0.5429 | 0.6238 |
Endosome | 0.0628 | 0.0549 | 0.0615 | 0.0553 | 0.0989 | 0.0765 | 0.0495 | 0.0461 | 0.0488 | 0.028 | 0.0366 | 0.0454 | 0.0406 | 0.0226 | 0.0386 | 0.0089 | 0.0757 | 0.0353 |
Golgi | 0.0148 | 0.0077 | 0.0256 | 0.0049 | 0.0171 | 0.0141 | 0.0268 | 0.0074 | 0.0142 | 0.0149 | 0.0118 | 0.0079 | 0.0073 | 0.0052 | 0.0082 | 0.0009 | 0.0088 | 0.0091 |
Lipid Particles | 0.0376 | 0.0115 | 0.0189 | 0.0023 | 0.0089 | 0.0109 | 0.0225 | 0.0174 | 0.0077 | 0.0068 | 0.0043 | 0.0063 | 0.019 | 0.0133 | 0.0146 | 0.0022 | 0.0157 | 0.0068 |
Mitochondria | 0.0142 | 0.003 | 0.0204 | 0.054 | 0.0628 | 0.0102 | 0.0201 | 0.0024 | 0.0124 | 0.0233 | 0.0294 | 0.0148 | 0.0039 | 0.0032 | 0.0028 | 0.0014 | 0.0053 | 0.0019 |
None | 0.0219 | 0.0145 | 0.0291 | 0.0025 | 0.0052 | 0.0071 | 0.0139 | 0.0033 | 0.0094 | 0.0069 | 0.007 | 0.0026 | 0.0327 | 0.0107 | 0.0148 | 0.0054 | 0.0035 | 0.0114 |
Nuclear Periphery | 0.0335 | 0.0165 | 0.0168 | 0.0128 | 0.0461 | 0.0161 | 0.0191 | 0.014 | 0.0127 | 0.0106 | 0.0175 | 0.014 | 0.0207 | 0.0116 | 0.0175 | 0.0159 | 0.0225 | 0.0091 |
Nucleolus | 0.0016 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0013 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0001 |
Nucleus | 0.0086 | 0.0102 | 0.0043 | 0.003 | 0.0085 | 0.0053 | 0.0021 | 0.0022 | 0.0037 | 0.0011 | 0.0036 | 0.0024 | 0.0037 | 0.004 | 0.0029 | 0.0024 | 0.008 | 0.0034 |
Peroxisomes | 0.0095 | 0.0003 | 0.006 | 0.0003 | 0.0043 | 0.0061 | 0.0238 | 0.0003 | 0.0007 | 0.0033 | 0.0006 | 0.0006 | 0.0079 | 0.0007 | 0.006 | 0 | 0.0054 | 0.0005 |
Punctate Nuclear | 0.0037 | 0.0012 | 0.0014 | 0.0006 | 0.0015 | 0.0014 | 0.001 | 0.0016 | 0.0007 | 0.0003 | 0.0018 | 0.0004 | 0.0065 | 0.0013 | 0.0026 | 0.0003 | 0.0074 | 0.0012 |
Vacuole | 0.0187 | 0.0114 | 0.0083 | 0.0076 | 0.0176 | 0.0083 | 0.0061 | 0.006 | 0.0077 | 0.0027 | 0.0053 | 0.0048 | 0.005 | 0.0064 | 0.0047 | 0.0047 | 0.0082 | 0.0046 |
Vacuole Periphery | 0.0044 | 0.0012 | 0.0019 | 0.002 | 0.0046 | 0.0022 | 0.0029 | 0.001 | 0.0016 | 0.0008 | 0.0015 | 0.0016 | 0.0011 | 0.0006 | 0.0011 | 0.0009 | 0.0019 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.6196 | 11.5772 | 8.2471 | 18.1508 | 16.7912 | 19.6743 | 19.1973 | 18.4873 | 17.8158 | 18.5351 |
Translational Efficiency | 1.5062 | 1.2365 | 1.7267 | 0.922 | 0.771 | 0.7786 | 0.9617 | 0.6847 | 0.8539 | 0.9725 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
919 | 537 | 1106 | 1460 | 1889 | 524 | 1149 | 109 | 2808 | 1061 | 2255 | 1569 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 824.12 | 943.99 | 1123.89 | 1098.68 | 874.87 | 898.03 | 1108.27 | 1080.83 | 858.26 | 921.29 | 1115.93 | 1097.44 |
Standard Deviation | 387.80 | 135.80 | 155.37 | 134.02 | 124.39 | 135.38 | 144.99 | 122.02 | 245.35 | 137.53 | 150.38 | 133.30 |
Intensity Change Log 2 | — | 0.195917 | 0.447575 | 0.414845 | — | 0.037695 | 0.341169 | 0.304999 | — | 0.116611 | 0.393763 | 0.359326 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003950 | 0.006894 | 0.004545 | 0.036743 | 0.002311 | 0.005067 | 0.009539 | 0.028681 | 0.002848 | 0.005992 | 0.007090 | 0.036183 |
Bud Neck | 0.005009 | 0.006739 | 0.008277 | 0.019201 | 0.003078 | 0.005521 | 0.011250 | 0.007165 | 0.003710 | 0.006137 | 0.009792 | 0.018365 |
Bud Site | 0.018678 | 0.029657 | 0.026238 | 0.094239 | 0.009814 | 0.021323 | 0.033064 | 0.055822 | 0.012715 | 0.025541 | 0.029716 | 0.091570 |
Cell Periphery | 0.012634 | 0.002945 | 0.000612 | 0.010111 | 0.002137 | 0.006374 | 0.002617 | 0.004289 | 0.005573 | 0.004639 | 0.001634 | 0.009706 |
Cytoplasm | 0.161587* | 0.112293* | 0.196290* | 0.178175* | 0.182592* | 0.126023* | 0.176701* | 0.114695* | 0.175717* | 0.119074* | 0.186309* | 0.173765* |
Cytoplasmic Foci | 0.073078 | 0.110205 | 0.080359 | 0.051101 | 0.091940 | 0.085038 | 0.041468 | 0.094909 | 0.085767 | 0.097776 | 0.060543 | 0.054144 |
Eisosomes | 0.004094 | 0.001513 | 0.000231 | 0.004192 | 0.001276 | 0.001783 | 0.001040 | 0.001962 | 0.002198 | 0.001646 | 0.000643 | 0.004037 |
Endoplasmic Reticulum | 0.491825* | 0.532286* | 0.232592* | 0.356709* | 0.498106* | 0.543378* | 0.468103* | 0.339401* | 0.496050* | 0.537764* | 0.352593* | 0.355507* |
Endosome | 0.014368 | 0.029153 | 0.041335 | 0.020564 | 0.018136 | 0.026404 | 0.016993 | 0.027375 | 0.016903 | 0.027796 | 0.028932 | 0.021037 |
Golgi | 0.066837 | 0.052023 | 0.004115 | 0.015345 | 0.056759 | 0.060419 | 0.005209 | 0.005514 | 0.060058 | 0.056169 | 0.004672 | 0.014662 |
Lipid Particles | 0.063781 | 0.044665 | 0.073878 | 0.065024 | 0.061625 | 0.054638 | 0.049371 | 0.072299 | 0.062331 | 0.049591 | 0.061390 | 0.065530 |
Mitochondria | 0.031869 | 0.016650 | 0.000739 | 0.002775 | 0.020082 | 0.020465 | 0.000905 | 0.000574 | 0.023940 | 0.018534 | 0.000824 | 0.002622 |
Mitotic Spindle | 0.000163 | 0.000890 | 0.008012 | 0.009735 | 0.000686 | 0.000331 | 0.002630 | 0.004601 | 0.000515 | 0.000614 | 0.005270 | 0.009378 |
None | 0.002144 | 0.000656 | 0.001584 | 0.000546 | 0.005778 | 0.001424 | 0.000502 | 0.000044 | 0.004588 | 0.001035 | 0.001033 | 0.000511 |
Nuclear Periphery | 0.000386 | 0.000490 | 0.022554 | 0.000639 | 0.001380 | 0.000779 | 0.006052 | 0.002200 | 0.001055 | 0.000633 | 0.014146 | 0.000747 |
Nuclear Periphery Foci | 0.001785 | 0.003477 | 0.121217 | 0.013940 | 0.005066 | 0.002694 | 0.056559 | 0.057855 | 0.003993 | 0.003090 | 0.088272 | 0.016991 |
Nucleolus | 0.000104 | 0.000139 | 0.000558 | 0.000234 | 0.000343 | 0.000160 | 0.000188 | 0.000090 | 0.000265 | 0.000149 | 0.000369 | 0.000224 |
Nucleus | 0.004627 | 0.003615 | 0.036393 | 0.024593 | 0.010179 | 0.003567 | 0.037029 | 0.010302 | 0.008362 | 0.003592 | 0.036717 | 0.023600 |
Peroxisomes | 0.029743 | 0.010456 | 0.002385 | 0.006073 | 0.007728 | 0.011746 | 0.002270 | 0.008942 | 0.014933 | 0.011093 | 0.002327 | 0.006273 |
Vacuole | 0.006046 | 0.023252 | 0.136750 | 0.087182 | 0.014409 | 0.014520 | 0.077935 | 0.162289 | 0.011672 | 0.018939 | 0.106781 | 0.092400 |
Vacuole Periphery | 0.007291 | 0.012001 | 0.001335 | 0.002877 | 0.006575 | 0.008345 | 0.000574 | 0.000990 | 0.006809 | 0.010195 | 0.000947 | 0.002746 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.31 | -0.54 | -13.32 | -10.99 | -12.40 | -2.36 | -5.31 | -3.04 | -2.68 | -2.13 | -3.62 | -4.87 | -14.21 | -12.21 | -11.76 |
Bud Neck | -1.20 | -2.78 | -10.18 | -7.46 | -7.22 | -2.17 | -6.52 | -3.79 | -1.23 | 2.71 | -2.73 | -7.23 | -12.68 | -9.13 | -6.29 |
Bud Site | -2.71 | -2.23 | -15.06 | -11.20 | -12.57 | -3.61 | -7.05 | -4.01 | -2.95 | -1.95 | -5.13 | -7.38 | -17.88 | -13.45 | -12.74 |
Cell Periphery | 6.67 | 8.53 | 1.88 | -8.61 | -12.98 | -3.88 | -0.70 | -1.28 | 1.28 | -0.98 | 1.24 | 6.82 | -4.41 | -5.45 | -10.91 |
Cytoplasm | 6.27 | -3.88 | -2.57 | -8.76 | 1.71 | 7.10 | 0.61 | 4.46 | 0.48 | 3.91 | 10.20 | -1.86 | -0.09 | -8.62 | 1.49 |
Cytoplasmic Foci | -5.17 | -1.52 | 5.01 | 8.85 | 7.24 | 1.17 | 12.35 | -0.23 | -0.71 | -3.84 | -2.57 | 7.64 | 9.38 | 9.28 | 2.29 |
Eisosomes | 3.82 | 6.26 | 0.09 | -5.48 | -10.38 | -2.04 | 1.39 | -0.95 | -0.13 | -1.35 | 1.96 | 6.82 | -4.14 | -5.78 | -9.25 |
Endoplasmic Reticulum | -2.16 | 19.77 | 10.66 | 10.88 | -9.76 | -2.64 | 3.80 | 5.23 | 6.12 | 3.66 | -3.34 | 16.60 | 14.57 | 14.34 | -0.19 |
Endosome | -7.07 | -14.96 | -4.10 | 3.94 | 10.54 | -3.31 | 0.79 | -2.50 | -0.24 | -2.77 | -6.62 | -10.02 | -2.91 | 3.76 | 5.43 |
Golgi | 2.92 | 18.03 | 14.22 | 9.63 | -8.16 | -0.75 | 24.42 | 22.39 | 12.09 | -0.22 | 1.14 | 30.54 | 21.69 | 13.62 | -7.82 |
Lipid Particles | 4.29 | -2.03 | -0.35 | -4.75 | 1.74 | 1.40 | 3.20 | -0.90 | -1.45 | -1.91 | 3.86 | 0.38 | -0.98 | -4.09 | -1.24 |
Mitochondria | 4.10 | 11.60 | 10.74 | 5.41 | -4.13 | -0.11 | 16.09 | 15.93 | 6.26 | 1.02 | 2.29 | 19.39 | 16.96 | 7.74 | -3.89 |
Mitotic Spindle | -2.04 | -4.94 | -5.54 | -5.03 | -0.69 | 1.01 | -3.26 | -1.17 | -1.29 | -0.44 | -0.32 | -5.66 | -5.40 | -5.37 | -2.13 |
None | 2.53 | 0.78 | 3.05 | 0.39 | 1.59 | 4.48 | 6.21 | 6.74 | 2.78 | 3.59 | 5.42 | 5.39 | 6.72 | 1.72 | 1.50 |
Nuclear Periphery | -0.75 | -10.55 | -2.00 | -1.06 | 10.40 | 1.20 | -5.85 | -1.03 | -1.68 | 3.78 | 1.49 | -11.63 | 1.35 | -0.53 | 11.99 |
Nuclear Periphery Foci | -2.65 | -23.66 | -13.10 | -9.77 | 20.77 | 3.02 | -16.23 | -4.48 | -4.69 | -0.07 | 1.54 | -27.68 | -10.19 | -11.22 | 21.76 |
Nucleolus | -0.69 | -7.53 | -2.61 | -1.45 | 3.98 | 2.55 | 3.01 | 5.78 | 1.17 | 3.44 | 2.43 | -2.64 | 0.54 | -1.34 | 2.42 |
Nucleus | 0.90 | -13.25 | -10.96 | -13.93 | 3.88 | 9.87 | -12.91 | -0.34 | -4.63 | 10.69 | 8.12 | -18.33 | -10.56 | -14.28 | 5.79 |
Peroxisomes | 8.40 | 14.18 | 12.17 | 3.42 | -7.17 | -2.89 | 9.43 | -0.33 | 1.20 | -2.59 | 3.24 | 16.49 | 10.32 | 5.02 | -8.09 |
Vacuole | -6.76 | -26.19 | -21.93 | -15.76 | 6.93 | -0.04 | -17.49 | -7.70 | -7.68 | -4.02 | -4.25 | -30.30 | -21.89 | -19.24 | 2.36 |
Vacuole Periphery | -2.76 | 7.97 | 5.02 | 5.69 | -2.53 | -1.43 | 12.40 | 10.92 | 6.35 | -1.47 | -3.25 | 14.40 | 6.59 | 6.99 | -3.26 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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