Standard name
Human Ortholog
Description GTPase-activating protein for Gpa1p; regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.15 0.08 0.16 0.22 0.09 0.05 0 0 0 0 0 0 0.09 0.07 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.98 0.97 0.93 0.92 0.91 0.92 0.88 0.87 0.81 0.87 1.0 0.99 0.98 0.93 0.86 0.9 0.85 0.86 0.89 0.92 0.91 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 0 0.15 0.16 0.26 0.15 0 0 0 0.11 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 2 9 9 5 4 3 2 1 0 0 0 0 1 0 0 7 2 11 0 1 5
Bud 4 2 8 2 4 3 9 3 8 9 17 6 6 2 1 1 0 0 8 9 9 0 5 4
Bud Neck 0 0 1 1 1 3 7 4 3 7 4 11 0 0 0 2 0 0 4 3 6 0 2 1
Bud Site 0 0 0 0 4 1 3 1 2 1 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 38 21 78 85 35 31 23 6 9 13 6 4 36 31 9 3 4 0 8 1 7 0 0 1
Cytoplasm 250 253 464 376 374 542 607 450 426 462 361 487 391 447 482 113 61 45 225 225 436 173 354 385
Endoplasmic Reticulum 0 5 3 3 6 11 17 3 7 2 3 0 1 5 6 6 1 4 2 0 0 5 11 10
Endosome 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 3 2 1 3 1 0 1 2
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 1 0 1 0 0 2
Mitochondria 1 5 1 1 2 28 29 21 73 87 115 81 0 1 0 14 3 0 0 3 5 2 0 2
Nucleus 0 1 3 2 3 4 2 4 2 5 6 3 1 0 1 2 0 0 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 1 0 3 0 1 0 0 0 0 0 0 0 0 1 0 0 2
Nucleolus 0 0 0 1 0 0 2 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 0 3 1 1 0 6 0 0 0 1 3 0 0 1 3 0 0 0
Vac/Vac Membrane 0 1 1 1 5 13 9 14 4 10 12 12 0 0 0 0 3 1 2 3 1 2 6 5
Unique Cell Count 255 263 475 389 403 592 667 489 483 533 447 557 392 451 490 122 71 50 266 263 491 189 390 431
Labelled Cell Count 295 288 560 474 444 648 713 514 538 602 527 612 435 486 500 149 79 52 266 263 491 189 390 431


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 7.2 7.4 6.6 6.5 5.1 5.1 5.0 4.0 3.9 3.7 4.2 7.0 6.8 6.4 5.6 5.8 6.6 8.6 9.8 11.0
Std Deviation (1e-4) 2.1 1.9 1.4 1.2 1.2 1.1 1.4 1.0 0.9 1.0 0.7 1.0 1.7 1.6 1.3 2.0 1.7 2.6 1.9 1.9 2.1
Intensity Change (Log2) – – – -0.17 -0.18 -0.52 -0.52 -0.56 -0.87 -0.91 -0.99 -0.82 -0.07 -0.12 -0.2 -0.4 -0.35 -0.17 0.23 0.42 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -0.9 0 -0.5 -1.6 0 0 0 0 -0.2 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.0 -3.4 -6.0 -7.6 -8.4 0 0 0 0 -3.1 -4.5 -7.9 -4.0 -2.4 -3.1
Cytoplasm -0.9 -3.5 -4.3 -4.6 -4.0 -5.7 -6.4 -8.4 -6.1 2.6 1.7 0.8 -2.8 -4.8 -3.0
Endoplasmic Reticulum 0 0 1.7 2.4 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.5 4.3 4.2 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.8 0 3.3 0 2.5 3.2 2.8 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8797 1.9316 1.4795 1.557 1.0232 1.4383 2.971 3.0307 2.614 1.5431 2.1805 2.6011 1.8943 1.2917 1.4453 1.3203 1.4254 1.2223
Actin 0.0297 0.0008 0.0068 0.01 0.0087 0.008 0.0399 0.002 0.0153 0.0081 0.0094 0.0088 0.0381 0.0101 0.0288 0.0931 0.0045 0.0093
Bud 0.0013 0.0054 0.0029 0.0007 0.0012 0.0012 0.0016 0.0021 0.0072 0.0226 0.0049 0.0005 0.0009 0.0037 0.0018 0.0005 0.0007 0.001
Bud Neck 0.0047 0.001 0.0049 0.0008 0.0153 0.0179 0.0018 0.001 0.0028 0.0047 0.0041 0.0133 0.0033 0.0005 0.0032 0.0014 0.0013 0.0061
Bud Periphery 0.0025 0.0028 0.0055 0.001 0.0056 0.0032 0.0025 0.0021 0.0048 0.0284 0.0055 0.0015 0.0013 0.0032 0.0021 0.0012 0.0007 0.002
Bud Site 0.0069 0.0251 0.012 0.0019 0.0033 0.0016 0.0073 0.0342 0.0095 0.0181 0.0024 0.0003 0.0129 0.0241 0.021 0.0011 0.0081 0.0006
Cell Periphery 0.003 0.0039 0.0017 0.0008 0.0042 0.0033 0.0045 0.0075 0.003 0.0031 0.0015 0.0033 0.002 0.0061 0.003 0.0058 0.0007 0.0012
Cytoplasm 0.616 0.6784 0.665 0.8058 0.4649 0.7404 0.722 0.8752 0.8144 0.695 0.8257 0.8815 0.6997 0.7651 0.7017 0.7947 0.8143 0.8395
Cytoplasmic Foci 0.0289 0.0028 0.0084 0.004 0.0411 0.015 0.0169 0.0024 0.0126 0.0065 0.0119 0.0029 0.016 0.0037 0.0064 0.0089 0.0072 0.0029
Eisosomes 0.0006 0.0001 0.0002 0.0001 0.0009 0.0001 0.0005 0.0001 0.0002 0.0001 0.0007 0.0001 0.0004 0.0001 0.0002 0.0002 0 0
Endoplasmic Reticulum 0.0083 0.0026 0.0042 0.0062 0.0027 0.0033 0.0052 0.0024 0.0035 0.0052 0.0028 0.0019 0.0047 0.0031 0.0039 0.0091 0.0042 0.0025
Endosome 0.0208 0.0008 0.0074 0.002 0.0319 0.0024 0.0256 0.0004 0.0112 0.0095 0.0101 0.0008 0.0051 0.0037 0.0015 0.0058 0.0241 0.001
Golgi 0.0063 0.0003 0.0029 0.0004 0.0061 0.0011 0.0109 0.0002 0.0086 0.0008 0.012 0.0008 0.0061 0.0004 0.002 0.0015 0.0059 0.0011
Lipid Particles 0.0087 0.0002 0.0055 0.0005 0.008 0.0038 0.0114 0.0001 0.0041 0.0004 0.0039 0.0015 0.0032 0.0004 0.0043 0.0007 0.0032 0.0014
Mitochondria 0.0073 0.0002 0.0028 0.0007 0.0483 0.0003 0.0078 0.0001 0.005 0.0022 0.003 0.0007 0.008 0.0002 0.0014 0.0006 0.0029 0.0003
None 0.2014 0.272 0.2506 0.1512 0.3307 0.1908 0.1072 0.0676 0.0844 0.175 0.0962 0.0797 0.1879 0.1681 0.206 0.0378 0.1095 0.1248
Nuclear Periphery 0.0131 0.0004 0.0012 0.0049 0.0006 0.0007 0.0061 0.0003 0.0012 0.0037 0.0005 0.0002 0.0005 0.0022 0.0006 0.0113 0.0006 0.0002
Nucleolus 0.0006 0.0002 0.0004 0.0002 0.0004 0.0001 0.0028 0.0001 0.0004 0.0005 0.0001 0 0.0003 0.0001 0.0002 0.0005 0.0004 0
Nucleus 0.0123 0.0013 0.0027 0.0056 0.0011 0.0016 0.011 0.0013 0.0017 0.0083 0.0015 0.0008 0.0004 0.0024 0.0008 0.0089 0.0007 0.0004
Peroxisomes 0.0187 0.0001 0.0098 0.0002 0.014 0.0021 0.0075 0.0001 0.0058 0.0005 0.0016 0.0008 0.0075 0.0003 0.0097 0.0029 0.0016 0.0048
Punctate Nuclear 0.0039 0.0003 0.0018 0.0017 0.0084 0.0021 0.0044 0.0004 0.002 0.0041 0.0011 0.0002 0.0008 0.0014 0.0007 0.0131 0.0003 0.0005
Vacuole 0.0033 0.0014 0.0023 0.001 0.0018 0.0008 0.0021 0.0006 0.0018 0.0026 0.001 0.0004 0.0009 0.0011 0.0006 0.0008 0.0079 0.0003
Vacuole Periphery 0.0016 0.0001 0.0007 0.0002 0.0006 0.0001 0.001 0 0.0003 0.0006 0.0002 0 0.0002 0.0001 0.0001 0.0003 0.0013 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.4687 8.4667 16.4048 35.8328 26.2654 16.3026 14.5057 16.8522 18.515 29.7369
Translational Efficiency 0.2205 0.379 0.5283 0.3517 0.5707 0.2512 0.2606 0.3205 0.3854 0.3294

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1898 613 231 1068 1610 211 67 2054 3508 824 298 3122

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 678.94 964.73 1141.41 809.23 810.61 981.87 1009.36 819.66 739.37 969.12 1111.72 816.09
Standard Deviation 78.59 181.05 206.29 104.99 126.73 191.69 120.40 114.40 122.55 183.99 198.21 111.38
Intensity Change Log 2 — 0.506841 0.749461 0.253266 — 0.276524 0.316361 0.016018 — 0.386080 0.529977 0.129013

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000398 0.004207 0.004130 0.000502 0.000617 0.007992 0.000539 0.001030 0.000498 0.005176 0.003323 0.000849
Bud Neck 0.026387 0.011104 0.005823 0.012552 0.008747 0.018056 0.001584 0.010639 0.018291 0.012884 0.004870 0.011294
Bud Site 0.014219 0.084800 0.009944 0.051025 0.017312 0.074395 0.004499 0.040851 0.015638 0.082136 0.008720 0.044332
Cell Periphery 0.000315 0.000884 0.000666 0.001046 0.000542 0.001699 0.000384 0.000146 0.000419 0.001093 0.000603 0.000454
Cytoplasm 0.555828* 0.619382* 0.311502* 0.449980* 0.647161* 0.466184* 0.340396* 0.558529* 0.597745* 0.580153* 0.317998* 0.521395*
Cytoplasmic Foci 0.191703* 0.131921 0.014243 0.023256 0.135834 0.201328* 0.053746 0.016849 0.166062 0.149694 0.023124 0.019041
Eisosomes 0.000375 0.000244 0.000090 0.000046 0.000271 0.000419 0.000170 0.000034 0.000327 0.000289 0.000108 0.000038
Endoplasmic Reticulum 0.000716 0.000803 0.010913 0.002534 0.001484 0.000922 0.007896 0.000814 0.001068 0.000834 0.010234 0.001402
Endosome 0.012046 0.008053 0.005462 0.005753 0.008137 0.022109 0.010045 0.006239 0.010252 0.011652 0.006493 0.006073
Golgi 0.002655 0.004066 0.000312 0.003711 0.001382 0.006235 0.000151 0.006375 0.002071 0.004621 0.000276 0.005464
Lipid Particles 0.006619 0.002733 0.003852 0.001159 0.003623 0.001762 0.006237 0.000571 0.005244 0.002485 0.004388 0.000772
Mitochondria 0.003461 0.000518 0.000478 0.006168 0.000476 0.002017 0.000172 0.003582 0.002091 0.000902 0.000409 0.004467
Mitotic Spindle 0.001624 0.001654 0.001642 0.039944 0.000624 0.002042 0.000178 0.047084 0.001165 0.001753 0.001312 0.044641
None 0.004950 0.000544 0.003995 0.006896 0.002767 0.000859 0.000865 0.006156 0.003949 0.000625 0.003291 0.006409
Nuclear Periphery 0.000269 0.000050 0.001859 0.001827 0.000169 0.000078 0.001668 0.001340 0.000223 0.000057 0.001816 0.001507
Nuclear Periphery Foci 0.001218 0.002197 0.008890 0.002221 0.001743 0.001796 0.008104 0.000386 0.001459 0.002094 0.008713 0.001014
Nucleolus 0.000986 0.000936 0.000364 0.000925 0.000305 0.000316 0.000401 0.000755 0.000673 0.000777 0.000372 0.000813
Nucleus 0.098801 0.017803 0.439339* 0.259028* 0.078118 0.020295 0.260073* 0.207707* 0.089309 0.018441 0.399034* 0.225263*
Peroxisomes 0.003491 0.003392 0.000355 0.001266 0.001580 0.008296 0.000581 0.000898 0.002614 0.004647 0.000406 0.001024
Vacuole 0.073154 0.104360 0.175891 0.126381 0.088739 0.162225 0.302080* 0.086562 0.080307 0.119178 0.204262* 0.100183
Vacuole Periphery 0.000783 0.000349 0.000252 0.003778 0.000371 0.000973 0.000233 0.003454 0.000594 0.000509 0.000248 0.003564

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.15 -2.51 -0.25 4.16 2.50 -1.73 0.16 0.29 1.75 0.22 -3.67 -2.45 -0.02 3.64 2.42
Bud Neck 7.14 12.00 9.54 1.10 -1.68 -3.00 8.29 0.46 3.21 -9.68 3.54 12.36 9.24 2.80 -3.96
Bud Site -11.61 1.44 -2.74 9.19 -3.64 -5.69 6.56 -1.91 5.17 -7.46 -12.84 3.92 -3.50 11.13 -6.44
Cell Periphery -5.36 -2.80 -0.30 1.64 0.95 -2.31 3.41 11.58 3.24 5.52 -4.48 -1.82 2.27 5.10 2.98
Cytoplasm -5.18 20.73 21.84 21.22 -5.45 9.55 13.01 22.71 0.54 -6.62 2.01 26.34 28.16 15.61 -12.08
Cytoplasmic Foci 8.85 41.75 42.02 20.58 -1.20 -4.79 13.59 34.94 14.82 3.61 2.99 44.37 54.27 25.27 2.28
Eisosomes 3.77 9.99 11.80 6.87 4.32 -2.08 3.47 13.60 5.78 8.24 1.58 11.60 17.00 9.25 8.76
Endoplasmic Reticulum -0.61 -12.45 -14.60 -13.56 8.99 1.75 -6.04 0.19 -2.86 6.20 1.45 -13.43 -7.70 -11.60 12.04
Endosome 3.72 6.58 11.46 4.98 5.74 -3.81 -0.90 7.54 5.33 5.86 -0.77 5.10 13.39 7.16 7.69
Golgi -0.82 9.43 2.93 1.81 -1.88 -2.35 8.64 -1.32 1.74 -3.07 -1.96 11.83 0.29 1.94 -3.34
Lipid Particles 2.81 3.09 9.65 0.93 8.01 4.08 -2.69 6.54 4.50 6.59 2.73 0.69 11.98 1.40 10.98
Mitochondria 4.08 4.20 0.97 -2.07 -2.09 -1.34 2.50 -3.70 -0.04 -4.60 2.51 4.29 -0.00 -2.47 -4.19
Mitotic Spindle 0.04 -0.14 -6.48 -6.35 -6.52 -1.45 1.08 -10.82 -9.83 -11.04 -0.60 -0.47 -12.41 -11.59 -12.35
None 5.39 2.39 0.20 -8.07 -2.99 2.08 3.60 -4.23 -6.43 -12.95 6.13 2.71 -2.21 -13.93 -6.68
Nuclear Periphery 3.16 -15.21 -14.22 -17.12 -0.63 2.90 -8.75 -16.99 -17.93 -1.98 4.14 -18.39 -21.14 -24.23 -1.10
Nuclear Periphery Foci -1.65 -8.24 -0.85 1.18 8.47 -0.27 -4.76 9.84 4.52 5.93 -1.71 -9.64 2.08 4.84 10.94
Nucleolus -0.04 3.42 2.02 0.57 -1.25 0.01 -0.63 -4.31 -1.60 -3.23 -0.25 3.24 0.96 0.42 -3.37
Nucleus 28.86 -17.01 -25.83 -35.97 3.66 17.89 -5.85 -35.26 -45.04 -1.27 34.56 -17.75 -42.83 -59.19 3.95
Peroxisomes 0.25 12.03 9.22 5.19 -1.14 -4.23 4.91 4.96 5.02 0.29 -3.36 12.92 11.11 7.40 -1.15
Vacuole -6.34 -12.28 -19.32 -12.92 1.85 -6.42 -8.92 -13.42 0.55 6.81 -8.16 -14.56 -23.65 -9.47 7.19
Vacuole Periphery 5.70 7.30 -1.10 -2.17 -2.31 -2.24 3.93 -2.79 -1.00 -3.21 1.28 7.80 -2.64 -2.95 -3.85
Standard name
Human Ortholog
Description GTPase-activating protein for Gpa1p; regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
Localization
Cell Percentages cytoplasm (73%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Sst2

Sst2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sst2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available