Standard name
Human Ortholog
Description Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.12 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0.07
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0.06 0 0.05 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.19 0.13 0.11 0.07 0 0 0 0 0 0 0 0.25 0.22 0.21 0.11 0.13 0.12 0.08 0.13 0.07 0.11 0.09 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.65 0.4 0.54 0.56 0.46 0.45 0.35 0.25 0.14 0.13 0.16 0.1 0.64 0.65 0.56 0.2 0.17 0.17 0.19 0.22 0.2 0.21 0.2 0.23
Golgi 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.07 0 0 0 0 0.13 0.07
Mitochondria 0.05 0 0 0 0.05 0.12 0.13 0.17 0.32 0.26 0.29 0.24 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.15 0.16 0.23 0.33 0.44 0.48 0.48 0.51 0.52 0.57 0.58 0.11 0.18 0.31 0.48 0.47 0.49 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0.06 0.11 0.08 0.09 0.1 0.05 0.07 0 0 0.1 0 0 0 0 0 0 0 0 0
SpindlePole 0.12 0.15 0.22 0.29 0.38 0.31 0.4 0.29 0.27 0.38 0.34 0.3 0.16 0.23 0.29 0.5 0.46 0.36 0.5 0.43 0.45 0.38 0.33 0.3
Vac/Vac Membrane 0.11 0.16 0.1 0.05 0.09 0 0 0.05 0 0 0 0 0 0.06 0.08 0.08 0.12 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 47 15 13 5 4 6 0 2 0 0 2 4 2 1 22 7 10 7 14 19 16 13 25
Bud 1 2 5 3 8 8 6 4 15 8 4 5 1 0 1 2 0 3 8 20 14 16 22 23
Bud Neck 0 0 1 0 1 5 2 4 5 7 3 2 0 0 0 1 0 2 2 4 3 1 5 13
Bud Site 1 0 0 1 0 7 4 4 18 24 6 11 0 0 0 1 0 4
Cell Periphery 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 33 74 40 23 20 9 10 17 9 6 3 8 57 86 76 70 39 43 27 60 24 21 31 24
Endoplasmic Reticulum 8 16 8 2 1 1 0 1 0 1 0 0 0 2 0 5 4 12 4 3 5 4 3 4
Endosome 185 156 166 120 123 149 142 104 56 49 27 22 146 254 208 133 50 60 65 104 70 43 72 80
Golgi 4 29 7 7 0 0 1 0 0 0 0 0 8 2 0 36 32 24 7 5 3 4 44 25
Mitochondria 14 0 8 8 13 39 51 71 126 98 49 55 1 0 0 49 12 25 2 5 11 1 6 11
Nucleus 0 0 2 1 0 1 2 7 3 7 1 2 0 0 0 6 2 2 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 1 0 1 2 0 0 0 0 0 0 3 4 2 3 2 2
Nucleolus 63 59 48 49 89 146 195 198 202 198 97 133 26 69 116 321 141 171 2 7 2 1 1 1
Peroxisomes 2 1 6 7 14 20 45 33 34 37 9 16 3 11 37 18 5 11 1 3 3 3 12 14
SpindlePole 34 60 68 63 103 104 163 121 106 145 57 68 36 91 108 332 138 126 168 203 160 77 119 106
Vac/Vac Membrane 31 64 31 11 23 5 13 22 7 3 5 4 10 22 29 52 36 28 3 8 4 1 3 2
Unique Cell Count 285 389 306 216 270 331 407 415 395 380 170 229 229 391 369 662 301 347 338 473 359 204 358 352
Labelled Cell Count 384 508 406 308 400 498 642 588 585 583 262 330 292 539 576 1048 466 521 338 473 359 204 358 352


Cytoplasm, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 14.2 11.2 10.9 10.5 9.9 9.5 9.2 9.2 9.1 9.1 8.9 12.2 11.7 11.1 16.1 17.5 18.9 10.9 10.6 11.4
Std Deviation (1e-4) 2.1 2.3 2.5 2.1 2.2 1.9 2.1 2.0 2.2 2.3 2.3 2.5 2.2 2.0 2.2 4.1 4.9 6.0 2.0 2.1 2.0
Intensity Change (Log2) -0.04 -0.1 -0.18 -0.24 -0.29 -0.28 -0.31 -0.3 -0.33 0.12 0.06 -0.01 0.52 0.64 0.75 -0.04 -0.08 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 -2.0 -2.7 -2.7 -4.6 -3.8 -4.4 -2.9 -2.6 -2.0 -3.7 -3.9 -1.2 -1.7 -1.3
Bud 0 1.1 0.7 0 0 1.7 0.4 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.8 4.5 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -2.2 -4.9 -5.5 -4.4 -5.6 -6.0 -4.1 -3.8 3.5 3.0 2.6 -1.1 0 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 -1.1 0.6
Endosome 0.3 -2.1 -2.3 -5.2 -8.0 -11.3 -11.6 -8.2 -10.7 2.2 2.9 0.6 -10.7 -9.7 -9.9
Golgi 0.7 0 0 0 0 0 0 0 0 0.8 0 0 2.2 4.2 2.8
Mitochondria 0.7 1.4 4.4 4.8 6.2 9.8 8.3 8.4 7.6 0 0 0 2.9 0.9 2.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.0 4.9 7.8 9.0 9.0 9.7 9.9 9.4 10.3 -1.4 0.7 4.7 9.8 8.3 9.1
Peroxisomes 0.9 2.1 2.6 4.7 3.5 3.8 4.2 2.0 2.9 0 0.7 4.3 0.7 -0.3 1.0
SpindlePole 1.8 4.2 2.6 5.0 2.1 1.4 4.5 2.7 2.0 -1.9 0.3 2.1 8.2 6.1 3.9
Vacuole -2.1 -0.7 -4.7 -3.8 -2.5 -4.8 -5.6 -2.8 -3.9 -2.5 -2.2 -1.0 -1.2 0.7 -0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.1533 8.4021 7.0524 6.9938 6.8383 7.2086 7.5777 10.3956 9.7306 8.8256 8.7324 10.2477 10.04 12.922 11.9124 11.2881 10.9178 12.203
Actin 0.0069 0.0053 0.003 0.005 0.0252 0.0042 0.0488 0.0113 0.0316 0.0038 0.0631 0.0137 0.0186 0.002 0.0081 0.0121 0.0103 0.0065
Bud 0.001 0.0017 0.0005 0.0004 0.001 0.0005 0.0007 0.0009 0.0023 0.0009 0.0016 0.0045 0.0004 0.0015 0.0008 0.0011 0.0006 0.0006
Bud Neck 0.0043 0.002 0.0108 0.0025 0.0439 0.0118 0.0052 0.002 0.0032 0.0019 0.0059 0.0027 0.0022 0.0028 0.0055 0.0258 0.0058 0.0089
Bud Periphery 0.0009 0.0006 0.0006 0.0003 0.0008 0.0002 0.0009 0.0003 0.0008 0.0022 0.0011 0.0012 0.0003 0.0005 0.0007 0.0007 0.0004 0.0003
Bud Site 0.0082 0.0369 0.0102 0.0013 0.0024 0.0007 0.0087 0.012 0.0026 0.002 0.0039 0.0013 0.0052 0.0155 0.0091 0.0024 0.0012 0.001
Cell Periphery 0.0006 0.0003 0.0003 0 0.0001 0.0001 0.0005 0 0.0001 0.0004 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0
Cytoplasm 0.0741 0.2201 0.0456 0.1117 0.3783 0.2772 0.0586 0.1393 0.0669 0.0302 0.22 0.1668 0.1202 0.3164 0.1024 0.1361 0.2499 0.214
Cytoplasmic Foci 0.383 0.2618 0.3696 0.3451 0.163 0.294 0.3507 0.3939 0.3533 0.3864 0.2164 0.3877 0.2768 0.1888 0.2598 0.1917 0.1835 0.2722
Eisosomes 0.0001 0.0001 0.0003 0 0.0001 0 0.0007 0 0.0005 0.0002 0.0006 0.0001 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.016 0.0042 0.002 0.0052 0.031 0.0064 0.0033 0.0023 0.0014 0.003 0.0156 0.0037 0.0099 0.0023 0.0016 0.0049 0.0121 0.0049
Endosome 0.0717 0.0145 0.0099 0.0687 0.1905 0.0665 0.084 0.0424 0.0276 0.026 0.1417 0.0768 0.0833 0.0268 0.0259 0.0376 0.1557 0.0631
Golgi 0.023 0.0047 0.0034 0.0171 0.0202 0.0129 0.0181 0.0061 0.0068 0.007 0.0288 0.0142 0.0095 0.0048 0.0061 0.01 0.0239 0.0095
Lipid Particles 0.0173 0.0166 0.004 0.0118 0.0082 0.0087 0.0435 0.0019 0.0058 0.0169 0.0232 0.0084 0.0132 0.0032 0.0067 0.0132 0.041 0.0055
Mitochondria 0.0042 0.0008 0.0016 0.0028 0.0095 0.0026 0.0062 0.0006 0.0018 0.0489 0.016 0.0043 0.0016 0.0009 0.0043 0.0083 0.0049 0.0041
None 0.0018 0.002 0.0086 0.0012 0.0021 0.0014 0.0102 0.002 0.002 0.0347 0.0046 0.0017 0.0116 0.0018 0.0032 0.0026 0.0043 0.0021
Nuclear Periphery 0.0044 0.0039 0.0043 0.0055 0.0255 0.0126 0.0138 0.0039 0.0025 0.0031 0.0318 0.008 0.0184 0.0096 0.0038 0.009 0.0383 0.0129
Nucleolus 0.0017 0.012 0.0063 0.0049 0.0017 0.001 0.006 0.0025 0.0016 0.0024 0.0027 0.0007 0.0048 0.0046 0.0036 0.0024 0.0048 0.0027
Nucleus 0.0096 0.0245 0.0269 0.0054 0.0091 0.0266 0.0075 0.0155 0.0086 0.0035 0.0114 0.016 0.024 0.0704 0.031 0.0421 0.0281 0.0427
Peroxisomes 0.0205 0.0061 0.0066 0.0056 0.0232 0.0056 0.0519 0.02 0.0252 0.0207 0.0432 0.0233 0.0109 0.0096 0.01 0.0134 0.0442 0.015
Punctate Nuclear 0.3455 0.38 0.4839 0.4024 0.0466 0.2623 0.2753 0.3419 0.4546 0.4039 0.157 0.2628 0.3838 0.3348 0.5138 0.4833 0.1808 0.331
Vacuole 0.0043 0.0017 0.0015 0.0022 0.0116 0.0035 0.0037 0.0009 0.0007 0.0015 0.0069 0.0015 0.0042 0.003 0.0028 0.0022 0.0068 0.0022
Vacuole Periphery 0.0012 0.0003 0.0003 0.0009 0.0059 0.0012 0.0015 0.0003 0.0003 0.0006 0.0043 0.0006 0.0009 0.0005 0.0005 0.0008 0.0033 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 148.4401 162.1844 137.853 99.3164 97.2987 149.1821 182.6191 157.4653 135.8985 95.4909
Translational Efficiency 0.9651 1.0508 0.9046 1.09 0.8694 0.7982 0.9211 0.8989 0.7754 0.8014

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
856 1605 416 1037 1938 2120 2348 1444 2794 3725 2764 2481

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 960.41 1156.18 1473.33 1552.95 1102.77 1149.13 1481.02 1651.31 1059.16 1152.17 1479.86 1610.20
Standard Deviation 131.01 204.72 217.69 299.40 162.37 335.92 206.40 327.22 166.89 286.87 208.16 319.60
Intensity Change Log 2 0.267644 0.617358 0.693289 0.059410 0.425459 0.582479 0.160091 0.517971 0.635122

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000770 0.000425 0.000161 0.000477 0.000271 0.000267 0.000015 0.000552 0.000424 0.000335 0.000037 0.000521
Bud Neck 0.003379 0.002791 0.003933 0.002421 0.003381 0.002826 0.000728 0.002288 0.003381 0.002811 0.001211 0.002344
Bud Site 0.017536 0.011998 0.005154 0.008052 0.009386 0.013243 0.000903 0.010055 0.011883 0.012707 0.001543 0.009218
Cell Periphery 0.000105 0.000078 0.000044 0.000049 0.000062 0.000136 0.000005 0.000042 0.000075 0.000111 0.000011 0.000045
Cytoplasm 0.054891 0.052746 0.009473 0.011665 0.021840 0.076729 0.017677 0.032529 0.031966 0.066395 0.016442 0.023809
Cytoplasmic Foci 0.212457* 0.109709 0.014131 0.018859 0.077342 0.089664 0.002168 0.015230 0.118737 0.098301 0.003969 0.016747
Eisosomes 0.000021 0.000007 0.000011 0.000003 0.000010 0.000006 0.000000 0.000003 0.000013 0.000007 0.000002 0.000003
Endoplasmic Reticulum 0.000961 0.000362 0.000155 0.000091 0.000493 0.000710 0.000046 0.000129 0.000637 0.000560 0.000062 0.000113
Endosome 0.035301 0.012390 0.001408 0.001895 0.013948 0.012125 0.000444 0.004324 0.020490 0.012239 0.000589 0.003309
Golgi 0.012212 0.006685 0.000636 0.001393 0.003752 0.006308 0.000083 0.005712 0.006344 0.006470 0.000167 0.003907
Lipid Particles 0.016272 0.005364 0.000775 0.002411 0.005801 0.003334 0.000145 0.001135 0.009009 0.004209 0.000240 0.001668
Mitochondria 0.001074 0.001114 0.000243 0.000366 0.001331 0.001926 0.000054 0.000737 0.001252 0.001576 0.000082 0.000582
Mitotic Spindle 0.555174* 0.742612* 0.944663* 0.940869* 0.802562* 0.759007* 0.970516* 0.910675* 0.726770* 0.751943* 0.966625* 0.923295*
None 0.001400 0.000972 0.000415 0.000404 0.000408 0.002593 0.000262 0.000898 0.000712 0.001894 0.000285 0.000692
Nuclear Periphery 0.000330 0.000258 0.000644 0.000358 0.000493 0.000449 0.000285 0.000599 0.000443 0.000366 0.000339 0.000498
Nuclear Periphery Foci 0.065599 0.039332 0.003078 0.004103 0.046880 0.017482 0.001281 0.002853 0.052615 0.026897 0.001551 0.003375
Nucleolus 0.003292 0.001612 0.004517 0.000762 0.001959 0.001502 0.000404 0.000386 0.002367 0.001550 0.001023 0.000543
Nucleus 0.003804 0.002839 0.004125 0.001408 0.001422 0.003283 0.002595 0.002094 0.002152 0.003092 0.002825 0.001807
Peroxisomes 0.009979 0.003670 0.003206 0.001026 0.005511 0.003101 0.000092 0.001890 0.006880 0.003346 0.000561 0.001529
Vacuole 0.004309 0.004319 0.003039 0.003121 0.002745 0.004241 0.002245 0.006483 0.003224 0.004275 0.002365 0.005078
Vacuole Periphery 0.001134 0.000717 0.000190 0.000266 0.000402 0.001068 0.000051 0.001386 0.000626 0.000917 0.000072 0.000918

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.14 2.29 1.26 -0.32 -0.39 -0.04 8.07 -1.42 -1.40 -2.72 1.30 7.16 -0.73 -1.32 -3.34
Bud Neck 0.33 -5.92 -1.72 -1.96 4.83 0.99 3.85 0.04 -0.74 -2.91 1.10 0.76 -0.82 -1.74 -1.42
Bud Site 1.85 0.36 1.55 -0.08 1.06 -3.06 7.55 -1.87 0.45 -5.90 -1.48 7.53 -0.86 0.32 -6.43
Cell Periphery 2.02 -0.50 0.99 -0.44 1.22 -2.43 8.13 1.90 3.08 -6.46 -1.97 9.01 1.40 2.62 -5.45
Cytoplasm 0.17 5.89 3.85 4.10 -2.56 -10.87 -3.15 -8.70 1.01 -6.54 -9.10 0.38 -5.49 2.84 -5.70
Cytoplasmic Foci 9.41 18.68 19.57 13.64 -0.79 -2.82 20.10 14.75 17.25 -6.39 2.83 28.72 22.56 21.92 -7.72
Eisosomes 6.88 1.17 9.51 4.26 4.47 6.18 12.66 9.50 4.54 -4.26 8.73 11.90 13.21 6.16 0.74
Endoplasmic Reticulum 4.10 2.53 6.13 5.32 3.64 -0.34 6.36 6.28 1.59 0.02 0.65 7.57 8.75 2.18 2.17
Endosome 8.58 13.56 13.54 10.40 -0.01 2.13 15.35 10.96 8.51 -5.22 7.27 20.23 17.46 13.01 -5.58
Golgi 4.93 11.82 11.31 6.99 0.30 -2.74 12.68 0.18 1.70 -3.40 0.52 17.31 5.16 4.39 -3.72
Lipid Particles 6.33 9.46 7.29 1.85 -4.76 4.03 10.95 6.86 3.65 -6.84 6.86 14.37 9.36 3.67 -8.96
Mitochondria -0.03 1.90 1.79 1.02 -0.00 -0.88 2.70 1.45 2.04 -3.18 -0.64 3.33 2.11 2.20 -3.18
Mitotic Spindle -11.46 -19.11 -23.15 -14.23 -0.77 3.23 -19.25 -7.46 -10.39 9.27 -3.16 -28.76 -18.91 -16.99 8.62
None 0.95 1.93 2.26 1.67 -0.07 -4.57 -1.47 -1.82 2.09 -1.45 -4.09 1.05 -0.92 2.46 -1.45
Nuclear Periphery 1.14 -5.84 -3.24 -4.43 3.77 0.32 -1.93 -2.08 -1.47 -0.90 0.65 -4.01 -2.70 -2.28 0.43
Nuclear Periphery Foci 4.93 13.21 13.91 12.66 0.87 9.62 17.04 17.06 10.84 0.41 9.36 21.94 21.79 16.51 -0.62
Nucleolus 2.32 -2.35 3.14 1.58 4.02 2.34 3.01 3.59 1.77 1.08 3.25 1.61 4.74 2.38 3.04
Nucleus 0.97 -3.01 -0.05 -1.10 3.01 -3.50 -6.76 -5.82 -3.62 0.53 -1.92 -6.87 -4.94 -3.62 2.59
Peroxisomes 5.68 3.50 8.87 5.16 4.96 4.05 10.98 6.76 3.47 -3.36 6.50 12.65 10.96 5.85 -1.35
Vacuole -0.79 -3.22 -4.18 -3.96 0.62 -2.18 -5.15 -7.75 -7.10 -4.51 -2.41 -5.94 -8.83 -8.10 -3.77
Vacuole Periphery 1.26 2.77 3.13 2.26 1.00 -3.09 4.09 -2.07 0.06 -3.16 -1.81 5.13 -0.55 0.84 -3.29