Standard name
Description Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.12 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0.07
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0.06 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.19 0.13 0.11 0.07 0 0 0 0 0 0 0 0.25 0.22 0.21 0.11 0.13 0.12 0.08 0.13 0.07 0.11 0.09 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.65 0.4 0.54 0.56 0.46 0.45 0.35 0.25 0.14 0.13 0.16 0.1 0.64 0.65 0.56 0.2 0.17 0.17 0.19 0.22 0.2 0.21 0.2 0.23
Golgi 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.07 0 0 0 0 0.13 0.07
Mitochondria 0.05 0 0 0 0.05 0.12 0.13 0.17 0.32 0.26 0.29 0.24 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.15 0.16 0.23 0.33 0.44 0.48 0.48 0.51 0.52 0.57 0.58 0.11 0.18 0.31 0.48 0.47 0.49 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0.06 0.11 0.08 0.09 0.1 0.05 0.07 0 0 0.1 0 0 0 0 0 0 0 0 0
SpindlePole 0.12 0.15 0.22 0.29 0.38 0.31 0.4 0.29 0.27 0.38 0.34 0.3 0.16 0.23 0.29 0.5 0.46 0.36 0.5 0.43 0.45 0.38 0.33 0.3
Vac/Vac Membrane 0.11 0.16 0.1 0.05 0.09 0 0 0.05 0 0 0 0 0 0.06 0.08 0.08 0.12 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 47 15 13 5 4 6 0 2 0 0 2 4 2 1 22 7 10 7 14 19 16 13 25
Bud 1 2 5 3 8 8 6 4 15 8 4 5 1 0 1 2 0 3 8 20 14 16 22 23
Bud Neck 0 0 1 0 1 5 2 4 5 7 3 2 0 0 0 1 0 2 2 4 3 1 5 13
Bud Site 1 0 0 1 0 7 4 4 18 24 6 11 0 0 0 1 0 4 – – – – – –
Cell Periphery 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 33 74 40 23 20 9 10 17 9 6 3 8 57 86 76 70 39 43 27 60 24 21 31 24
Endoplasmic Reticulum 8 16 8 2 1 1 0 1 0 1 0 0 0 2 0 5 4 12 4 3 5 4 3 4
Endosome 185 156 166 120 123 149 142 104 56 49 27 22 146 254 208 133 50 60 65 104 70 43 72 80
Golgi 4 29 7 7 0 0 1 0 0 0 0 0 8 2 0 36 32 24 7 5 3 4 44 25
Mitochondria 14 0 8 8 13 39 51 71 126 98 49 55 1 0 0 49 12 25 2 5 11 1 6 11
Nucleus 0 0 2 1 0 1 2 7 3 7 1 2 0 0 0 6 2 2 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 1 1 0 1 2 0 0 0 0 0 0 3 4 2 3 2 2
Nucleolus 63 59 48 49 89 146 195 198 202 198 97 133 26 69 116 321 141 171 2 7 2 1 1 1
Peroxisomes 2 1 6 7 14 20 45 33 34 37 9 16 3 11 37 18 5 11 1 3 3 3 12 14
SpindlePole 34 60 68 63 103 104 163 121 106 145 57 68 36 91 108 332 138 126 168 203 160 77 119 106
Vac/Vac Membrane 31 64 31 11 23 5 13 22 7 3 5 4 10 22 29 52 36 28 3 8 4 1 3 2
Unique Cell Count 285 389 306 216 270 331 407 415 395 380 170 229 229 391 369 662 301 347 338 473 359 204 358 352
Labelled Cell Count 384 508 406 308 400 498 642 588 585 583 262 330 292 539 576 1048 466 521 338 473 359 204 358 352


Cytoplasm, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 14.2 11.2 10.9 10.5 9.9 9.5 9.2 9.2 9.1 9.1 8.9 12.2 11.7 11.1 16.1 17.5 18.9 10.9 10.6 11.4
Std Deviation (1e-4) 2.1 2.3 2.5 2.1 2.2 1.9 2.1 2.0 2.2 2.3 2.3 2.5 2.2 2.0 2.2 4.1 4.9 6.0 2.0 2.1 2.0
Intensity Change (Log2) – – – -0.04 -0.1 -0.18 -0.24 -0.29 -0.28 -0.31 -0.3 -0.33 0.12 0.06 -0.01 0.52 0.64 0.75 -0.04 -0.08 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 -2.0 -2.7 -2.7 -4.6 -3.8 -4.4 -2.9 -2.6 -2.0 -3.7 -3.9 -1.2 -1.7 -1.3
Bud 0 1.1 0.7 0 0 1.7 0.4 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.8 4.5 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.8 -2.2 -4.9 -5.5 -4.4 -5.6 -6.0 -4.1 -3.8 3.5 3.0 2.6 -1.1 0 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 -1.1 0.6
Endosome 0.3 -2.1 -2.3 -5.2 -8.0 -11.3 -11.6 -8.2 -10.7 2.2 2.9 0.6 -10.7 -9.7 -9.9
Golgi 0.7 0 0 0 0 0 0 0 0 0.8 0 0 2.2 4.2 2.8
Mitochondria 0.7 1.4 4.4 4.8 6.2 9.8 8.3 8.4 7.6 0 0 0 2.9 0.9 2.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.0 4.9 7.8 9.0 9.0 9.7 9.9 9.4 10.3 -1.4 0.7 4.7 9.8 8.3 9.1
Peroxisomes 0.9 2.1 2.6 4.7 3.5 3.8 4.2 2.0 2.9 0 0.7 4.3 0.7 -0.3 1.0
SpindlePole 1.8 4.2 2.6 5.0 2.1 1.4 4.5 2.7 2.0 -1.9 0.3 2.1 8.2 6.1 3.9
Vacuole -2.1 -0.7 -4.7 -3.8 -2.5 -4.8 -5.6 -2.8 -3.9 -2.5 -2.2 -1.0 -1.2 0.7 -0.9