Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex; subunit of Gtr1-Gtr2, a GTPase that activates TORC1 in response to amino acid stimulation; subunit of EGO/GSE, a vacuolar/endosomal membrane complex that regulates exit from rapamycin-induced growth arrest and sorting of Gap1p from the endosome to the plasma membrane; involved in phosphate transport and telomeric chromatin silencing; activated by the the Iml1p (GAP) subunit of the SEACIT complex; similar to human RagA and RagB |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 3 | 15 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 3 | 11 | 9 | 4 | 4 | 6 | 6 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 34 | 4 | 1 | 20 | 41 | 61 | 76 | 88 | 81 | 104 | 93 | 108 | 12 | 20 | 26 | 8 | 15 | 20 | 33 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 5 | 2 | 8 | 36 | 8 | 7 | 7 | 12 | 2 | 3 | 3 | 4 | 9 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 4 | 4 | 2 | 8 | 20 | 19 | 17 | 11 | 6 | 10 | 1 | 8 | 29 | 20 | 17 | 1 | 1 | 7 | 22 | 6 | 5 | 12 | 44 | 57 |
Golgi | 0 | 1 | 0 | 5 | 14 | 5 | 2 | 13 | 0 | 2 | 0 | 3 | 1 | 1 | 3 | 10 | 14 | 20 | 0 | 1 | 0 | 0 | 1 | 2 |
Mitochondria | 47 | 2 | 5 | 23 | 32 | 100 | 178 | 96 | 179 | 144 | 173 | 99 | 9 | 2 | 1 | 5 | 9 | 14 | 6 | 0 | 0 | 4 | 9 | 7 |
Nucleus | 13 | 9 | 5 | 8 | 17 | 19 | 12 | 10 | 6 | 12 | 2 | 9 | 36 | 22 | 39 | 5 | 2 | 4 | 5 | 6 | 1 | 4 | 19 | 18 |
Nuclear Periphery | 19 | 11 | 12 | 39 | 4 | 11 | 12 | 24 | 9 | 7 | 4 | 6 | 27 | 21 | 50 | 27 | 26 | 34 | 12 | 2 | 12 | 5 | 24 | 25 |
Nucleolus | 27 | 9 | 4 | 2 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 18 | 15 | 9 | 4 | 8 | 8 | 4 | 1 | 0 | 1 | 3 | 7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 3 |
Vac/Vac Membrane | 86 | 59 | 34 | 252 | 311 | 263 | 315 | 228 | 188 | 229 | 130 | 163 | 346 | 407 | 471 | 114 | 183 | 176 | 99 | 85 | 55 | 118 | 287 | 339 |
Unique Cell Count | 170 | 79 | 49 | 295 | 385 | 393 | 492 | 451 | 369 | 425 | 343 | 362 | 401 | 454 | 540 | 132 | 219 | 213 | 187 | 106 | 77 | 149 | 398 | 471 |
Labelled Cell Count | 231 | 99 | 63 | 360 | 448 | 486 | 632 | 527 | 481 | 523 | 431 | 425 | 482 | 513 | 623 | 179 | 270 | 294 | 187 | 106 | 77 | 149 | 398 | 471 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.3 | 9.3 | 7.3 | 8.5 | 8.4 | 7.0 | 6.7 | 7.4 | 6.5 | 6.9 | 6.3 | 7.1 | 9.5 | 9.8 | 9.8 | 15.8 | 17.0 | 16.8 | 9.2 | 9.5 | 10.5 |
Std Deviation (1e-4) | 9.3 | 2.1 | 1.3 | 1.7 | 1.5 | 1.6 | 1.3 | 1.3 | 1.2 | 1.2 | 1.2 | 1.3 | 1.7 | 1.7 | 1.7 | 4.1 | 4.7 | 4.5 | 2.0 | 2.0 | 2.6 |
Intensity Change (Log2) | – | – | – | 0.23 | 0.21 | -0.04 | -0.11 | 0.04 | -0.16 | -0.07 | -0.2 | -0.04 | 0.38 | 0.43 | 0.43 | 1.12 | 1.23 | 1.21 | 0.34 | 0.39 | 0.53 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 2.6 | 2.6 | 3.0 | 3.3 | 3.6 | 3.8 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.4 | 3.7 | 1.8 | 5.1 | 3.4 | 5.3 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.3 | -0.6 | -2.3 | -1.4 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.8 | 1.9 | -0.3 | -0.7 | -2.5 | -2.4 | -2.1 | -4.2 | -3.2 | 3.1 | 4.1 | 3.4 | 2.6 | 2.3 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.0059 | 3.4384 | 3.2791 | 3.0297 | 2.707 | 3.2001 | 3.1516 | 4.2493 | 3.892 | 3.5486 | 3.2139 | 3.8099 | 2.284 | 3.1778 | 2.8166 | 2.2688 | 2.0659 | 2.8571 |
Actin | 0.0029 | 0 | 0.0087 | 0 | 0.0008 | 0 | 0.0151 | 0 | 0.0088 | 0 | 0.0084 | 0.0129 | 0.0124 | 0 | 0.0127 | 0.0152 | 0 | 0 |
Bud | 0.0003 | 0.001 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0.0003 | 0.0089 | 0.0004 | 0 | 0 |
Bud Neck | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0012 | 0.0001 | 0.0005 | 0.001 | 0.0003 | 0.0002 | 0.0007 | 0.0002 | 0.0019 | 0.0073 | 0 | 0 |
Bud Periphery | 0.0017 | 0.0013 | 0.001 | 0.0002 | 0.0003 | 0.0006 | 0.0006 | 0.0003 | 0.0005 | 0.0006 | 0.0008 | 0.0002 | 0.0005 | 0.0004 | 0.0019 | 0.0006 | 0.0001 | 0.0003 |
Bud Site | 0.0004 | 0.0006 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0023 | 0.0006 | 0.0013 | 0.0225 | 0.001 | 0.0001 | 0.0017 | 0.0006 | 0.0028 | 0.0154 | 0 | 0 |
Cell Periphery | 0.0006 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0005 | 0.0006 | 0.0005 | 0.0004 | 0.0003 | 0.001 | 0.0003 | 0.001 | 0.0005 | 0.0001 | 0.0001 |
Cytoplasm | 0.003 | 0.0029 | 0.0018 | 0.0002 | 0.0014 | 0.0006 | 0.0098 | 0.0021 | 0.0013 | 0.0008 | 0.0007 | 0.0005 | 0.007 | 0.0064 | 0.0017 | 0.0007 | 0.0005 | 0.0001 |
Cytoplasmic Foci | 0.0057 | 0.0017 | 0.0029 | 0.0004 | 0.0011 | 0.0014 | 0.02 | 0.003 | 0.0063 | 0.0009 | 0.0024 | 0.0049 | 0.0296 | 0.0037 | 0.0076 | 0.007 | 0.0002 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0026 | 0.0025 | 0.0006 | 0.0014 | 0.001 | 0.0036 | 0.0008 | 0.0039 | 0.0004 | 0.0019 | 0.0008 | 0.002 | 0.0009 | 0.001 | 0.0003 | 0.0008 | 0.0008 |
Endosome | 0.0941 | 0.0599 | 0.1012 | 0.0673 | 0.092 | 0.0727 | 0.1021 | 0.0424 | 0.0761 | 0.0587 | 0.0825 | 0.0452 | 0.0945 | 0.0992 | 0.0717 | 0.1066 | 0.0609 | 0.016 |
Golgi | 0.006 | 0.001 | 0.0036 | 0.001 | 0.0018 | 0.0021 | 0.0167 | 0.0009 | 0.0081 | 0.0009 | 0.0128 | 0.0067 | 0.0065 | 0.0025 | 0.0085 | 0.0032 | 0.0007 | 0.001 |
Lipid Particles | 0.0162 | 0.0081 | 0.0057 | 0.0036 | 0.0025 | 0.0034 | 0.0241 | 0.0023 | 0.0076 | 0.0008 | 0.0087 | 0.014 | 0.0187 | 0.0059 | 0.0029 | 0.0046 | 0.0004 | 0.0038 |
Mitochondria | 0.0253 | 0.0096 | 0.0164 | 0.001 | 0.0043 | 0.0059 | 0.0208 | 0.0027 | 0.0281 | 0.001 | 0.0421 | 0.0076 | 0.0229 | 0.0068 | 0.0365 | 0.0023 | 0.0009 | 0.0169 |
None | 0.0088 | 0.0002 | 0.0003 | 0 | 0.0037 | 0.0001 | 0.0077 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0094 | 0.0002 | 0.0003 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0303 | 0.024 | 0.0147 | 0.0098 | 0.0195 | 0.0199 | 0.0158 | 0.0259 | 0.0123 | 0.0026 | 0.0107 | 0.0096 | 0.0204 | 0.005 | 0.0129 | 0.0023 | 0.0071 | 0.0405 |
Nucleolus | 0.0069 | 0.0006 | 0.0012 | 0.0002 | 0.0015 | 0.0004 | 0.0043 | 0.0013 | 0.0011 | 0.0025 | 0.0009 | 0.0018 | 0.004 | 0.0018 | 0.0026 | 0.0006 | 0.0001 | 0.0002 |
Nucleus | 0.0081 | 0.0036 | 0.0051 | 0.0005 | 0.0053 | 0.0014 | 0.0095 | 0.0056 | 0.0017 | 0.002 | 0.0017 | 0.0007 | 0.0027 | 0.0013 | 0.0018 | 0.0005 | 0.0004 | 0.0006 |
Peroxisomes | 0.0036 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0007 | 0.0096 | 0.0002 | 0.0042 | 0.0002 | 0.0012 | 0.0026 | 0.0078 | 0.0003 | 0.0015 | 0.0067 | 0 | 0.0002 |
Punctate Nuclear | 0.002 | 0.0001 | 0.0006 | 0 | 0.0037 | 0 | 0.0139 | 0.0002 | 0.0016 | 0.0001 | 0.0004 | 0.0003 | 0.003 | 0 | 0 | 0.0001 | 0 | 0 |
Vacuole | 0.2971 | 0.3436 | 0.3303 | 0.3161 | 0.2656 | 0.2792 | 0.2849 | 0.4088 | 0.3533 | 0.3817 | 0.2979 | 0.2247 | 0.3161 | 0.3089 | 0.2853 | 0.3233 | 0.25 | 0.1667 |
Vacuole Periphery | 0.4832 | 0.5386 | 0.5016 | 0.5988 | 0.5943 | 0.61 | 0.4368 | 0.5019 | 0.482 | 0.522 | 0.5241 | 0.6666 | 0.4383 | 0.5552 | 0.5363 | 0.5021 | 0.6777 | 0.7527 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.9657 | 32.7735 | 17.2589 | 19.975 | 26.0669 | 16.5478 | 22.4478 | 26.3335 | 28.2449 | 24.5213 |
Translational Efficiency | 1.1844 | 0.5385 | 0.9965 | 1.0367 | 0.7414 | 1.1393 | 0.8452 | 0.5869 | 0.7415 | 0.7379 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1124 | 793 | 2787 | 1426 | 1827 | 1493 | 308 | 535 | 2951 | 2286 | 3095 | 1961 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 931.51 | 870.16 | 1032.68 | 1046.94 | 943.64 | 984.24 | 1322.84 | 1130.20 | 939.02 | 944.67 | 1061.56 | 1069.65 |
Standard Deviation | 148.53 | 176.21 | 148.10 | 140.99 | 139.09 | 138.82 | 180.36 | 174.11 | 142.88 | 162.19 | 174.74 | 155.25 |
Intensity Change Log 2 | — | -0.098291 | 0.148750 | 0.168536 | — | 0.060774 | 0.487330 | 0.260270 | — | -0.016054 | 0.329040 | 0.215428 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000055 | 0.000139 | 0.000033 | 0.000153 | 0.000005 | 0.000094 | 0.000147 | 0.000067 | 0.000024 | 0.000110 | 0.000044 | 0.000130 |
Bud Neck | 0.000996 | 0.002470 | 0.003027 | 0.003070 | 0.000182 | 0.002925 | 0.006413 | 0.004838 | 0.000492 | 0.002767 | 0.003364 | 0.003552 |
Bud Site | 0.004012 | 0.015839 | 0.003550 | 0.007424 | 0.001023 | 0.004558 | 0.005240 | 0.007173 | 0.002161 | 0.008471 | 0.003718 | 0.007356 |
Cell Periphery | 0.000335 | 0.000457 | 0.000416 | 0.000213 | 0.000352 | 0.000206 | 0.000330 | 0.000182 | 0.000346 | 0.000293 | 0.000407 | 0.000205 |
Cytoplasm | 0.004212 | 0.011978 | 0.000406 | 0.001131 | 0.001256 | 0.001005 | 0.000483 | 0.001745 | 0.002382 | 0.004811 | 0.000414 | 0.001298 |
Cytoplasmic Foci | 0.003197 | 0.019679 | 0.002903 | 0.008875 | 0.001873 | 0.010423 | 0.007271 | 0.002802 | 0.002377 | 0.013634 | 0.003338 | 0.007218 |
Eisosomes | 0.000007 | 0.000024 | 0.000023 | 0.000017 | 0.000001 | 0.000019 | 0.000028 | 0.000013 | 0.000003 | 0.000021 | 0.000023 | 0.000016 |
Endoplasmic Reticulum | 0.002037 | 0.001527 | 0.000665 | 0.000701 | 0.001088 | 0.001303 | 0.000558 | 0.000618 | 0.001449 | 0.001381 | 0.000655 | 0.000678 |
Endosome | 0.022262 | 0.064607 | 0.017323 | 0.037873 | 0.006972 | 0.054681 | 0.012907 | 0.022431 | 0.012796 | 0.058124 | 0.016883 | 0.033660 |
Golgi | 0.003129 | 0.020101 | 0.004309 | 0.028994 | 0.002379 | 0.010656 | 0.004630 | 0.007755 | 0.002665 | 0.013932 | 0.004341 | 0.023199 |
Lipid Particles | 0.000663 | 0.001171 | 0.000456 | 0.000849 | 0.000065 | 0.001509 | 0.000641 | 0.000203 | 0.000293 | 0.001392 | 0.000474 | 0.000673 |
Mitochondria | 0.008653 | 0.012256 | 0.004055 | 0.016575 | 0.005400 | 0.016297 | 0.006058 | 0.003807 | 0.006639 | 0.014896 | 0.004254 | 0.013092 |
Mitotic Spindle | 0.000764 | 0.001414 | 0.000670 | 0.002845 | 0.000424 | 0.005775 | 0.004411 | 0.002667 | 0.000553 | 0.004262 | 0.001042 | 0.002796 |
None | 0.000962 | 0.001090 | 0.000212 | 0.000235 | 0.000074 | 0.000304 | 0.000209 | 0.000334 | 0.000412 | 0.000577 | 0.000212 | 0.000262 |
Nuclear Periphery | 0.000476 | 0.001338 | 0.001603 | 0.001938 | 0.000257 | 0.004349 | 0.004582 | 0.001151 | 0.000341 | 0.003305 | 0.001899 | 0.001724 |
Nuclear Periphery Foci | 0.000469 | 0.003015 | 0.001147 | 0.002345 | 0.000095 | 0.009810 | 0.004683 | 0.000828 | 0.000237 | 0.007453 | 0.001499 | 0.001932 |
Nucleolus | 0.001073 | 0.003333 | 0.005661 | 0.003500 | 0.000135 | 0.007644 | 0.021007 | 0.003632 | 0.000493 | 0.006148 | 0.007188 | 0.003536 |
Nucleus | 0.001345 | 0.005803 | 0.004686 | 0.002732 | 0.000170 | 0.005265 | 0.008203 | 0.004587 | 0.000617 | 0.005452 | 0.005036 | 0.003238 |
Peroxisomes | 0.000466 | 0.002625 | 0.000555 | 0.000846 | 0.000037 | 0.002173 | 0.001665 | 0.000784 | 0.000201 | 0.002329 | 0.000666 | 0.000829 |
Vacuole | 0.275804* | 0.274571* | 0.362196* | 0.429648* | 0.224238* | 0.276250* | 0.393429* | 0.490294* | 0.243879* | 0.275667* | 0.365304* | 0.446193* |
Vacuole Periphery | 0.669082* | 0.556563* | 0.586104* | 0.450035* | 0.753973* | 0.584755* | 0.517106* | 0.444088* | 0.721639* | 0.574976* | 0.579238* | 0.448413* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.69 | 1.13 | -1.44 | -0.20 | -1.75 | -4.49 | -3.08 | -2.10 | 0.74 | 1.48 | -5.02 | -1.70 | -2.13 | -0.40 | -1.66 |
Bud Neck | -3.04 | -6.76 | -5.00 | -1.15 | -0.06 | -7.89 | -7.04 | -5.33 | -2.09 | 1.29 | -8.02 | -12.25 | -8.39 | -1.83 | -0.43 |
Bud Site | -5.94 | 0.63 | -3.39 | 3.92 | -4.04 | -4.57 | -3.13 | -4.70 | -1.77 | -0.98 | -7.29 | -3.73 | -6.81 | 0.93 | -4.44 |
Cell Periphery | -1.73 | -1.36 | 2.46 | 4.30 | 4.88 | 2.43 | 0.11 | 2.88 | 0.58 | 0.68 | 1.14 | -1.13 | 3.57 | 2.82 | 4.73 |
Cytoplasm | -3.61 | 3.83 | 2.97 | 5.58 | -3.27 | 0.61 | 2.21 | -1.26 | -1.62 | -2.32 | -3.06 | 4.52 | 1.72 | 4.53 | -3.90 |
Cytoplasmic Foci | -8.19 | 0.52 | -5.27 | 4.90 | -5.46 | -8.44 | -2.26 | -1.29 | 6.42 | 1.83 | -11.65 | -2.09 | -6.09 | 5.36 | -4.37 |
Eisosomes | -2.51 | -8.56 | -5.36 | 1.00 | 2.29 | -10.86 | -6.64 | -6.27 | 2.48 | 3.42 | -6.65 | -12.72 | -9.09 | 1.64 | 3.52 |
Endoplasmic Reticulum | 1.07 | 3.29 | 3.10 | 3.69 | -0.38 | -1.26 | 3.20 | 2.74 | 4.88 | -0.44 | 0.30 | 4.33 | 3.87 | 5.63 | -0.33 |
Endosome | -8.44 | 2.15 | -5.31 | 5.30 | -8.72 | -14.42 | -2.71 | -6.74 | 8.38 | -3.15 | -16.23 | -3.14 | -10.99 | 7.89 | -8.78 |
Golgi | -8.88 | -2.44 | -11.14 | -2.98 | -10.72 | -7.66 | -3.12 | -3.48 | 1.65 | -1.91 | -11.60 | -4.43 | -11.74 | -4.70 | -10.81 |
Lipid Particles | -1.46 | 0.85 | -0.68 | 1.17 | -3.10 | -5.89 | -3.46 | -3.85 | 5.25 | 2.55 | -5.34 | -1.86 | -2.98 | 3.54 | -2.06 |
Mitochondria | -1.50 | 2.86 | -3.29 | -1.63 | -6.35 | -5.25 | -0.23 | 1.08 | 5.98 | 0.77 | -5.18 | 2.27 | -3.92 | 0.94 | -5.78 |
Mitotic Spindle | -1.33 | 0.09 | -3.34 | -2.74 | -4.30 | -5.65 | -2.30 | -1.99 | 2.21 | 0.87 | -5.77 | -1.62 | -4.36 | 1.97 | -3.38 |
None | -0.19 | 1.19 | 1.15 | 3.54 | -1.36 | -7.57 | -7.37 | -1.54 | -0.18 | -0.74 | -0.65 | 0.83 | 0.61 | 3.20 | -1.03 |
Nuclear Periphery | -1.71 | -3.57 | -5.67 | -1.09 | -0.79 | -6.38 | -3.52 | -3.74 | 4.49 | 2.67 | -6.52 | -5.12 | -7.17 | 3.16 | 0.49 |
Nuclear Periphery Foci | -3.19 | -3.21 | -6.45 | 0.87 | -3.53 | -7.08 | -2.32 | -5.27 | 6.48 | 1.92 | -7.68 | -4.85 | -8.34 | 5.75 | -1.24 |
Nucleolus | -2.40 | -6.85 | -3.58 | -0.22 | 3.33 | -7.58 | -5.32 | -4.84 | 3.14 | 4.35 | -7.74 | -11.18 | -7.02 | 3.15 | 5.27 |
Nucleus | -3.04 | -6.16 | -2.39 | 1.96 | 2.73 | -7.13 | -5.26 | -4.16 | 0.22 | 1.77 | -6.97 | -9.90 | -5.42 | 2.42 | 2.55 |
Peroxisomes | -5.04 | -0.80 | -2.57 | 4.11 | -1.84 | -5.95 | -4.24 | -2.84 | 3.15 | 1.94 | -7.66 | -4.88 | -5.38 | 5.09 | -1.01 |
Vacuole | 0.10 | -11.33 | -17.96 | -17.28 | -9.42 | -7.88 | -12.72 | -23.69 | -18.90 | -6.04 | -5.88 | -22.10 | -32.05 | -26.51 | -12.51 |
Vacuole Periphery | 8.50 | 9.11 | 21.27 | 8.24 | 16.20 | 19.00* | 15.34 | 25.13 | 10.52 | 3.94 | 19.60 | 22.42 | 37.57 | 14.96 | 17.54 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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