Standard name
Human Ortholog
Description Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.07 0.08 0.13 0.14
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.05 0.13 0.08 0.07 0 0 0.16 0 0.06 0 0.08 0 0 0 0.22 0.21 0.18 0.17 0.36 0.36 0.3 0.32 0.29
Mitochondria 0.85 0.92 0.69 0.83 0.88 0.88 0.86 0.66 0.91 0.88 0.92 0.9 0.92 0.94 0.92 0.15 0.16 0.17 0.55 0.38 0.44 0.39 0.37 0.42
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0.18 0.19 0.15 0.22 0.29 0.28 0.31 0.24 0.18 0.17 0.12 0.18 0.15 0.12 0.26 0.27 0.3 0 0.06 0 0.06 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.26 0.16 0.26 0.11 0.24 0.23 0.16 0.16 0.11 0.1 0.08 0.07 0.24 0.25 0.19 0.42 0.34 0.4 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 2 1 3 5 6 2 8 3 8 0 0 1 0 0 0 11 12 23 15 39 50
Bud 0 0 1 1 1 2 2 1 0 0 1 1 0 1 1 0 0 0 0 1 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 5 3 1 0 0 1 0 4 1 1 1 4 2 7 8 1 5 1 1 0 3 2
Cytoplasm 9 8 8 7 2 1 1 4 5 6 2 3 8 3 2 27 15 14 1 4 0 1 0 0
Endoplasmic Reticulum 0 1 0 0 1 1 0 0 0 3 1 0 1 0 1 22 13 6 1 4 1 0 1 3
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 3 2
Golgi 0 10 30 15 18 7 17 100 5 27 10 30 1 7 8 72 40 27 24 98 113 54 97 101
Mitochondria 93 197 154 147 218 347 491 401 406 422 349 358 252 307 314 47 31 26 79 104 138 72 112 145
Nucleus 1 1 0 0 1 2 3 2 0 0 1 0 0 0 1 2 3 5 1 1 0 2 2 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 2 1 3 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 39 38 42 26 55 114 162 187 108 87 64 47 49 48 40 84 52 46 2 15 11 11 10 12
Peroxisomes 0 0 0 0 1 0 1 1 0 0 0 1 0 0 1 1 0 0 5 2 8 7 20 13
SpindlePole 0 0 0 0 1 0 0 0 0 2 0 0 0 0 0 2 1 0 0 0 0 0 0 0
Vac/Vac Membrane 29 35 58 20 61 92 90 99 51 48 29 29 66 81 64 136 65 61 0 12 3 7 2 3
Unique Cell Count 110 214 223 177 249 396 570 610 445 482 379 398 274 328 341 322 191 151 144 276 318 184 308 350
Labelled Cell Count 172 293 299 221 362 569 772 803 577 609 462 482 378 451 435 400 228 186 144 276 318 184 308 350


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 176.3 144.3 134.7 129.6 100.5 83.4 69.3 65.5 59.1 53.5 48.3 49.4 140.9 132.1 121.3 154.2 160.2 147.8 129.3 136.8 139.9
Std Deviation (1e-4) 43.3 38.4 33.0 35.6 30.2 24.7 22.0 19.2 18.2 17.4 15.8 16.7 35.5 32.6 28.7 59.6 58.3 56.8 39.7 38.2 40.8
Intensity Change (Log2) – – – -0.06 -0.42 -0.69 -0.96 -1.04 -1.19 -1.33 -1.48 -1.45 0.07 -0.03 -0.15 0.2 0.25 0.13 -0.06 0.02 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1.1 0
Cytoplasm 0.2 0 0 0 0 0 0 0 0 -0.4 0 0 2.2 1.9 2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.0 4.0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -1.6 -2.2 -5.9 -5.6 1.0 -6.7 -3.6 -5.1 -2.4 -6.0 -5.2 -5.1 2.6 2.0 1.2
Mitochondria 3.2 4.9 5.6 5.6 -0.9 7.3 5.9 7.4 6.6 6.6 7.6 7.1 -13.0 -10.8 -9.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.1 0.9 2.7 2.8 3.4 1.6 -0.3 -0.6 -2.4 -0.3 -1.3 -2.3 2.0 2.0 2.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.7 -0.4 -0.8 -3.3 -3.2 -4.8 -5.5 -6.2 -6.4 -0.5 -0.3 -2.0 3.9 1.8 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 169.2444 195.1634 167.3263 159.8531 141.4156 181.2902 197.152 207.0227 183.003 155.306 180.0579 187.0813 203.9845 247.1402 213.7222 166.1827 222.908 230.6499
Actin 0.0047 0.0003 0.0011 0.0004 0.0109 0 0.0018 0.0003 0.0124 0.0208 0.0002 0.0002 0.0097 0.0001 0.0165 0.0004 0.0047 0.0001
Bud 0.0001 0 0 0 0.0004 0 0 0 0.0001 0.0001 0 0 0.0001 0 0.0001 0 0 0
Bud Neck 0.0001 0 0.0001 0.0003 0.0005 0 0 0 0.0001 0.0001 0 0 0.0001 0 0.0003 0 0.0001 0
Bud Periphery 0.0001 0 0 0 0.0006 0 0 0 0.0002 0.0005 0 0 0.0001 0 0.0002 0 0.0001 0
Bud Site 0.0005 0 0.0003 0 0.0042 0 0.0002 0 0.0004 0.0006 0 0 0.0002 0 0.0004 0 0.0001 0
Cell Periphery 0.0002 0.0002 0.0001 0.0003 0.0002 0 0.0003 0.0005 0.0004 0.0003 0.0001 0.0001 0.0002 0.0003 0.0004 0.0001 0 0
Cytoplasm 0.0005 0 0 0 0.0003 0 0.0001 0 0 0 0 0 0.0002 0 0.0001 0 0.0001 0
Cytoplasmic Foci 0.0089 0 0.0073 0.0001 0.0377 0 0.0083 0 0.0028 0.0021 0.001 0 0.0045 0 0.0066 0.0001 0.0016 0
Eisosomes 0.0029 0.0119 0.0037 0.0019 0.0014 0.0003 0.0069 0.003 0.005 0.005 0.0015 0.0014 0.0044 0.0033 0.0067 0.0021 0.0007 0.0017
Endoplasmic Reticulum 0.0003 0 0 0 0.0009 0 0 0 0 0.0003 0 0 0.0014 0 0.0002 0 0.0001 0
Endosome 0.0028 0 0.0003 0 0.0045 0 0 0 0.0004 0.002 0 0 0.0047 0 0.0014 0 0.0035 0
Golgi 0.0017 0 0.0004 0 0.0037 0 0.0001 0.0001 0.0013 0.0083 0.0003 0.0001 0.0033 0 0.003 0 0.0029 0
Lipid Particles 0.0067 0.0015 0.0058 0.0005 0.0132 0.0005 0.003 0.0029 0.0069 0.0869 0.0101 0.0013 0.0175 0.0012 0.0095 0.0015 0.0085 0.0001
Mitochondria 0.9332 0.9785 0.9646 0.9885 0.8337 0.9982 0.9505 0.985 0.9465 0.846 0.9679 0.9934 0.9223 0.9855 0.9282 0.9897 0.9727 0.9972
None 0.0021 0 0.0005 0 0.0025 0 0.0014 0 0.0001 0.0005 0.0001 0 0.0007 0 0.0012 0 0.0002 0
Nuclear Periphery 0.0015 0 0 0 0.0188 0 0.0001 0 0 0.0074 0.0001 0 0.0035 0 0.0002 0 0.0005 0
Nucleolus 0.0204 0.001 0.0063 0.0002 0.0471 0 0.0173 0.0006 0.005 0.0025 0.0033 0.0012 0.0053 0.0008 0.0112 0.0015 0.0001 0
Nucleus 0.0012 0.0001 0.0003 0 0.0034 0 0.0006 0.0001 0.0002 0.0009 0.0002 0.0001 0.0005 0.0001 0.0005 0.0002 0.0001 0
Peroxisomes 0.008 0.0008 0.0073 0.0074 0.0065 0.0002 0.0034 0.0008 0.0057 0.0048 0.0029 0.0005 0.0086 0.0012 0.0052 0.0034 0.0026 0.0005
Punctate Nuclear 0.0017 0 0.0002 0 0.0062 0 0.0001 0 0 0.0011 0 0 0.0032 0 0.0002 0 0.0007 0
Vacuole 0.0006 0.0006 0.0002 0.0001 0.0007 0 0.0003 0.0006 0.0015 0.0009 0.0003 0.0001 0.0005 0.0007 0.0007 0.0001 0.0001 0
Vacuole Periphery 0.0019 0.005 0.0014 0.0003 0.0025 0.0007 0.0055 0.006 0.0108 0.0088 0.0119 0.0016 0.0089 0.0067 0.007 0.0009 0.0007 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 247.684 209.761 180.6672 191.5908 203.7264 201.9462 227.2384 193.5594 164.1934 188.0417
Translational Efficiency 0.9389 1.1068 1.0706 0.9321 0.9473 1.2812 0.9626 0.9687 1.0883 1.0169

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
23 1321 2376 1483 1339 1299 136 795 1362 2620 2512 2278

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 293.87 9779.80 9597.26 7622.78 9268.07 10189.22 8795.10 7367.97 9116.52 9982.79 9553.83 7533.85
Standard Deviation 6.10 3312.08 3185.15 2016.75 2780.58 3160.14 2217.38 2097.99 2989.67 3244.11 3145.64 2049.07
Intensity Change Log 2 — 5.056555 5.029373 4.697067 — 0.136703 -0.075569 -0.331002 — 1.062388 0.943731 0.648697

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000045 0.005355 0.002276 0.005591 0.002290 0.005404 0.009610 0.008629 0.002252 0.005379 0.002673 0.006651
Bud Neck 0.000010 0.001811 0.001628 0.000937 0.001393 0.001345 0.012127 0.002832 0.001369 0.001580 0.002197 0.001598
Bud Site 0.000015 0.000915 0.002146 0.000603 0.001110 0.000893 0.009860 0.003533 0.001092 0.000904 0.002564 0.001625
Cell Periphery 0.000054 0.000169 0.002323 0.000125 0.000113 0.000188 0.000421 0.000776 0.000112 0.000179 0.002220 0.000352
Cytoplasm 0.000024 0.000134 0.000233 0.000157 0.000089 0.000175 0.001326 0.000313 0.000088 0.000154 0.000292 0.000212
Cytoplasmic Foci 0.000015 0.017942 0.008322 0.009576 0.023023 0.019398 0.154033 0.018242 0.022635 0.018664 0.016211 0.012600
Eisosomes 0.000055 0.016401 0.020192 0.005051 0.014388 0.013832 0.009590 0.004297 0.014146 0.015127 0.019618 0.004788
Endoplasmic Reticulum 0.000000 0.000087 0.000058 0.000053 0.000036 0.000047 0.000144 0.000461 0.000036 0.000067 0.000063 0.000195
Endosome 0.000001 0.000134 0.000182 0.000516 0.000075 0.000126 0.000513 0.001598 0.000074 0.000130 0.000200 0.000893
Golgi 0.000004 0.003517 0.000678 0.005585 0.002499 0.003918 0.005053 0.010241 0.002457 0.003716 0.000915 0.007210
Lipid Particles 0.000037 0.004219 0.001166 0.001163 0.002946 0.004868 0.006722 0.004983 0.002897 0.004541 0.001467 0.002496
Mitochondria 0.000008 0.912473* 0.927056* 0.953339* 0.911613* 0.903010* 0.687786* 0.881000* 0.896219* 0.907781* 0.914102* 0.928094*
Mitotic Spindle 0.000400 0.000381 0.000211 0.000494 0.000579 0.000425 0.010610 0.001865 0.000576 0.000403 0.000774 0.000972
None 0.999159* 0.007498 0.004997 0.003175 0.010529 0.009396 0.005219 0.007289 0.027224 0.008439 0.005009 0.004611
Nuclear Periphery 0.000000 0.000273 0.000263 0.000189 0.000382 0.000484 0.002882 0.001109 0.000375 0.000378 0.000404 0.000510
Nuclear Periphery Foci 0.000035 0.000288 0.000113 0.000070 0.000172 0.000190 0.000716 0.000346 0.000169 0.000239 0.000145 0.000166
Nucleolus 0.000027 0.002500 0.002787 0.000676 0.003492 0.003435 0.014165 0.002003 0.003434 0.002963 0.003403 0.001139
Nucleus 0.000008 0.000157 0.000428 0.000150 0.000150 0.000509 0.002168 0.001196 0.000148 0.000331 0.000522 0.000515
Peroxisomes 0.000102 0.009043 0.004475 0.006058 0.006702 0.007637 0.041988 0.005436 0.006591 0.008346 0.006506 0.005841
Vacuole 0.000001 0.006476 0.010446 0.002367 0.006536 0.009390 0.013875 0.013467 0.006426 0.007921 0.010632 0.006241
Vacuole Periphery 0.000000 0.010226 0.010020 0.004124 0.011883 0.015331 0.011191 0.030384 0.011683 0.012757 0.010083 0.013288

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.50 -8.66 -8.40 -0.20 -4.68 -2.86 -3.28 -3.12 -1.47 0.31 -3.74 -0.77 -4.67 -1.20 -4.64
Bud Neck -5.02 -6.01 -6.50 2.26 2.27 0.15 -5.03 -2.92 -3.23 4.29 -0.65 -2.24 -0.82 -0.17 1.77
Bud Site -4.14 -6.21 -5.42 1.26 4.28 0.45 -2.38 -2.52 -2.85 1.62 0.45 -2.63 -1.17 -2.09 1.61
Cell Periphery -3.99 -3.94 -1.99 1.03 3.81 -1.38 -1.70 -2.34 -2.06 -0.76 -1.79 -3.88 -2.31 -1.68 3.36
Cytoplasm -4.79 -4.13 -4.85 -0.79 1.29 -1.70 -1.47 -3.70 -1.86 1.38 -2.26 -2.35 -4.29 -1.62 1.40
Cytoplasmic Foci -9.43 -9.25 -7.02 3.45 -0.87 1.29 -7.24 1.27 0.19 7.43 1.64 2.53 4.10 3.08 1.75
Eisosomes -12.60 -14.84 -10.70 8.24 10.55 0.37 2.12 9.14 7.90 2.42 -0.74 -3.35 8.85 11.18 11.12
Endoplasmic Reticulum -2.17 -8.15 -6.57 0.84 0.52 -1.50 -2.11 -2.01 -1.96 -1.46 -1.52 -3.23 -2.16 -1.68 -1.78
Endosome -6.20 -6.09 -2.21 -1.63 -1.42 -1.75 -4.25 -1.78 -1.72 -1.26 -2.82 -4.17 -2.45 -2.28 -2.06
Golgi -6.80 -7.46 -7.42 -2.26 -6.48 -2.59 -2.48 -5.03 -4.01 -2.90 -2.76 4.90 -6.10 -4.37 -8.65
Lipid Particles -6.37 -7.43 -3.22 4.11 0.01 -2.50 -2.26 -1.87 -0.10 0.89 -2.85 3.89 0.73 3.17 -2.23
Mitochondria -194.06* -287.50* -300.68* -7.17 -5.77 1.30 7.75 3.51 2.50 -6.53 -1.79 -2.64 -4.80 -4.00 -2.74
Mitotic Spindle 0.08 1.50 -0.62 -0.65 -2.36 0.47 -3.43 -1.50 -1.71 2.85 0.58 -0.61 -1.03 -1.81 -0.65
None 2442.97* 3583.10* 3657.66* 11.28 7.51 1.63 6.60 4.32 3.30 -2.75 5.36 6.35 6.46 11.33 1.49
Nuclear Periphery -8.20 -8.00 -8.10 2.06 1.82 -1.33 -3.88 -5.22 -4.15 2.71 -0.05 -0.52 -2.19 -2.12 -1.49
Nuclear Periphery Foci -1.57 -3.79 -1.70 1.36 2.56 -0.45 -4.84 -2.81 -2.32 2.99 -0.83 0.93 0.07 0.85 -0.82
Nucleolus -6.03 -6.46 -7.81 4.34 4.83 0.07 -3.07 2.38 2.46 3.52 0.74 0.04 4.13 5.28 4.88
Nucleus -4.69 -3.56 -1.69 -0.05 1.78 -1.27 -2.02 -1.23 -0.80 1.36 -1.30 -3.06 -1.24 -0.61 0.24
Peroxisomes -7.43 -9.02 -6.84 2.01 -1.59 -0.63 -4.62 0.86 1.30 4.76 -1.46 0.11 0.67 2.26 0.66
Vacuole -7.38 -10.35 -5.02 4.11 7.25 -1.92 -3.22 -3.33 -1.80 0.65 -1.37 -3.47 0.13 1.52 3.63
Vacuole Periphery -7.69 -11.56 -7.94 4.27 5.84 -1.68 0.28 -5.09 -4.06 -5.07 -0.64 1.01 -0.88 -0.34 -2.13
Standard name
Human Ortholog
Description Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
Localization
Cell Percentages mitochondrion (64%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Aco2

Aco2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aco2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available