Standard name
Human Ortholog
Description Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 0 0.05 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.94 0.95 0.96 0.92 0.9 0.89 0.89 0.84 0.89 0.86 1.0 0.99 1.0 0.96 0.96 0.97 0.98 0.94 0.95 0.96 0.96 0.97
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0.06 0.06 0.08 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 2 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 4 4 13 3 19 16 13 13 14 35 0 1 2 3 1 2 0 0 0 1 0 0
Cytoplasm 446 245 83 247 375 165 382 488 252 332 299 400 270 382 528 188 149 140 464 227 85 181 245 382
Endoplasmic Reticulum 6 0 2 4 13 4 31 32 16 30 21 33 2 1 3 4 1 1 1 0 1 1 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
Golgi 0 1 0 1 0 0 0 4 2 5 0 3 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 0 0 0 0 0 4 0 0 2 4 0 3 0 0 0 2 2 3 1 0 0 0 0 0
Nucleus 1 0 4 1 2 2 3 12 5 9 6 7 0 0 0 0 3 1 0 5 0 0 0 3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 0 11 5 8 10 13 4 22 5 16 0 1 0 3 3 4 0 5 0 0 1 3
Unique Cell Count 452 245 88 261 392 179 426 546 283 395 337 465 271 385 529 195 156 144 475 243 90 188 256 396
Labelled Cell Count 459 250 93 268 408 186 445 566 296 416 347 497 272 386 534 202 160 152 475 243 90 188 256 396


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 29.4 36.0 28.0 30.3 28.5 17.0 23.0 21.9 21.1 21.0 22.0 22.7 34.8 31.7 31.3 29.6 31.0 32.2 31.2 33.7 32.4
Std Deviation (1e-4) 6.5 7.6 5.9 6.0 5.8 6.0 4.6 4.5 4.6 4.7 4.4 4.6 7.0 7.5 6.5 10.4 10.7 12.0 7.2 7.5 7.5
Intensity Change (Log2) – – – 0.11 0.02 -0.72 -0.29 -0.36 -0.41 -0.42 -0.35 -0.31 0.31 0.18 0.16 0.08 0.15 0.2 0.16 0.26 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.0 0 0 0 0 0 0
Cytoplasm 0.1 0.5 -0.6 -1.4 -1.4 -1.5 -2.5 -1.5 -2.1 3.4 3.2 4.9 0.8 0.4 1.1
Endoplasmic Reticulum 0 0 0 1.7 0 0 1.8 0 1.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.0603 30.3203 26.6957 25.5633 23.7794 29.0682 33.2857 42.4168 39.1389 37.6405 36.1803 40.4134 31.9066 41.176 35.4335 34.9896 31.8516 36.1487
Actin 0.0161 0.0006 0.0105 0.002 0.0363 0.0097 0.0457 0.0002 0.0013 0.0131 0.0003 0.0003 0.0171 0.0002 0.0116 0.0001 0.0181 0.0013
Bud 0.0008 0.0003 0.0003 0.0016 0.0003 0.0001 0.0016 0 0.0009 0.0028 0.0006 0.0002 0.0003 0 0.0002 0 0.0003 0.0001
Bud Neck 0.0011 0.0005 0.0004 0.0006 0.0007 0.001 0.0009 0.0001 0.0002 0.0012 0.0004 0.0005 0.0005 0.0001 0.0003 0.0002 0.0004 0.0013
Bud Periphery 0.0009 0.0001 0.0003 0.0014 0.0004 0.0001 0.0011 0 0.0004 0.0049 0.0016 0 0.0001 0 0.0003 0 0.0005 0.0001
Bud Site 0.0031 0.0002 0.0032 0.004 0.0008 0.0001 0.0075 0 0.0004 0.004 0.0002 0.0001 0.0002 0 0.0008 0 0.0013 0.0003
Cell Periphery 0.0002 0 0.0001 0.0004 0.0001 0 0.0003 0 0.0002 0.0008 0.0006 0 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.8611 0.9869 0.909 0.8735 0.8747 0.9514 0.8866 0.9958 0.966 0.8826 0.8866 0.9679 0.9111 0.9964 0.9366 0.9856 0.86 0.9588
Cytoplasmic Foci 0.0138 0.0005 0.0128 0.0064 0.004 0.0037 0.0018 0.0001 0.0044 0.0012 0.0003 0.0023 0.008 0.0001 0.0087 0.0004 0.0032 0.0018
Eisosomes 0.0001 0 0.0001 0.0004 0.0001 0.0001 0.0004 0 0 0.0001 0 0 0.0001 0 0.0001 0 0.0004 0
Endoplasmic Reticulum 0.0037 0.0005 0.0017 0.0016 0.0009 0.001 0.0087 0.0001 0.0025 0.0091 0.0006 0.0007 0.0058 0.0001 0.0034 0.0001 0.001 0.0003
Endosome 0.0138 0.0002 0.009 0.0202 0.0061 0.0035 0.0074 0 0.0028 0.0343 0.0046 0.0025 0.0046 0 0.002 0.0001 0.0112 0.0024
Golgi 0.0062 0 0.0035 0.002 0.0046 0.0017 0.0027 0 0.0005 0.0041 0.0003 0.0001 0.0005 0 0.0016 0 0.009 0.0031
Lipid Particles 0.0081 0 0.007 0.0087 0.0081 0.0002 0.0004 0 0.0014 0.0005 0.0001 0.0002 0.0026 0 0.0029 0 0.017 0.0057
Mitochondria 0.0092 0.0001 0.0042 0.025 0.0218 0.0009 0.0029 0 0.0012 0.004 0.0011 0.0003 0.0005 0 0.0087 0 0.0631 0.0027
None 0.0034 0.0003 0.0057 0.0038 0.0018 0.0002 0.0011 0.0001 0.0017 0.0007 0.0002 0.0008 0.0046 0.0001 0.0113 0.0002 0.0008 0.0002
Nuclear Periphery 0.0097 0.0014 0.0053 0.0035 0.0034 0.0036 0.0102 0.0003 0.0021 0.0113 0.0047 0.0034 0.0072 0.0002 0.0017 0.0008 0.0018 0.0018
Nucleolus 0.0012 0 0.0007 0.0021 0.0002 0 0.0002 0 0.0001 0.0003 0.0002 0.0004 0.0004 0 0.0001 0 0.0002 0.0003
Nucleus 0.0245 0.0068 0.0151 0.0189 0.0208 0.0216 0.0173 0.0032 0.0098 0.0099 0.0187 0.0159 0.0168 0.0026 0.0071 0.0122 0.0057 0.0174
Peroxisomes 0.0059 0 0.0058 0.0017 0.0108 0.0002 0.0003 0 0.0002 0.0001 0 0 0.0002 0 0.0016 0 0.0033 0.0014
Punctate Nuclear 0.0095 0.0001 0.0026 0.0013 0.0007 0.0002 0.0005 0.0001 0.0003 0.0003 0.0004 0.001 0.0107 0 0.0003 0.0002 0.0008 0.0003
Vacuole 0.0047 0.0013 0.0019 0.0147 0.0019 0.0005 0.0013 0 0.003 0.0088 0.0525 0.0027 0.0074 0 0.0004 0 0.0006 0.0004
Vacuole Periphery 0.003 0.0001 0.0007 0.0061 0.0014 0.0002 0.0011 0 0.0006 0.0059 0.0259 0.0008 0.0011 0 0.0003 0 0.0011 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 302.9906 330.2405 309.5402 346.959 266.7955 179.7505 305.3481 324.702 294.0953 250.5861
Translational Efficiency 1.0873 1.2805 1.3041 1.1031 1.15 1.6132 1.2474 1.0394 1.101 1.0587

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2324 934 1388 1940 1298 1921 696 217 3622 2855 2084 2157

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2102.99 2141.28 3008.47 2880.82 2068.42 2415.68 2831.67 2809.29 2090.60 2325.91 2949.42 2873.62
Standard Deviation 483.57 483.10 464.89 527.20 395.08 440.23 450.46 536.83 454.15 472.57 467.61 528.62
Intensity Change Log 2 — 0.026031 0.516588 0.454038 — 0.223900 0.453124 0.441676 — 0.127537 0.485468 0.447921

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000092 0.000299 0.000194 0.000382 0.000266 0.000202 0.000288 0.000730 0.000154 0.000234 0.000226 0.000417
Bud Neck 0.000925 0.000997 0.001621 0.002954 0.000669 0.001019 0.001701 0.003075 0.000833 0.001012 0.001647 0.002966
Bud Site 0.001903 0.002964 0.001928 0.006637 0.002011 0.005358 0.002537 0.013676 0.001942 0.004575 0.002131 0.007345
Cell Periphery 0.000109 0.000469 0.000587 0.000318 0.000403 0.000197 0.000559 0.001329 0.000215 0.000286 0.000578 0.000420
Cytoplasm 0.837882* 0.766840* 0.625236* 0.656279* 0.776084* 0.761447* 0.611795* 0.646274* 0.815736* 0.763212* 0.620747* 0.655273*
Cytoplasmic Foci 0.008346 0.010891 0.008665 0.005851 0.008611 0.010284 0.009208 0.037249 0.008441 0.010483 0.008846 0.009010
Eisosomes 0.000003 0.000002 0.000002 0.000002 0.000002 0.000003 0.000004 0.000011 0.000003 0.000002 0.000003 0.000003
Endoplasmic Reticulum 0.000150 0.000160 0.000078 0.000099 0.000214 0.000155 0.000270 0.000089 0.000173 0.000157 0.000142 0.000098
Endosome 0.000167 0.000301 0.000442 0.000858 0.000144 0.000619 0.001442 0.000159 0.000159 0.000515 0.000776 0.000787
Golgi 0.000603 0.001126 0.001687 0.000527 0.000864 0.000988 0.002480 0.000330 0.000697 0.001034 0.001952 0.000507
Lipid Particles 0.000391 0.000965 0.001306 0.001501 0.000768 0.000658 0.001501 0.007546 0.000526 0.000759 0.001371 0.002109
Mitochondria 0.000291 0.000174 0.000228 0.000327 0.000135 0.000179 0.000169 0.000102 0.000235 0.000177 0.000208 0.000305
Mitotic Spindle 0.000715 0.000573 0.003942 0.036453 0.000406 0.003364 0.000760 0.001560 0.000604 0.002451 0.002879 0.032943
None 0.008436 0.011290 0.012435 0.009904 0.013582 0.016092 0.019674 0.030372 0.010280 0.014521 0.014852 0.011963
Nuclear Periphery 0.000046 0.000100 0.000305 0.000481 0.000130 0.000082 0.000214 0.000183 0.000077 0.000088 0.000275 0.000451
Nuclear Periphery Foci 0.000793 0.003000 0.001548 0.002156 0.002902 0.001625 0.001586 0.003653 0.001549 0.002075 0.001561 0.002307
Nucleolus 0.000149 0.000664 0.000116 0.000226 0.000084 0.000132 0.000101 0.000097 0.000126 0.000306 0.000111 0.000213
Nucleus 0.126603 0.191435* 0.327837* 0.256941* 0.185319* 0.187408* 0.331203* 0.219374* 0.147645* 0.188725* 0.328961* 0.253161*
Peroxisomes 0.000085 0.000050 0.000016 0.000127 0.000014 0.000102 0.000013 0.000081 0.000059 0.000085 0.000015 0.000122
Vacuole 0.011445 0.007266 0.011620 0.016568 0.006218 0.009835 0.014401 0.034055 0.009572 0.008995 0.012549 0.018327
Vacuole Periphery 0.000864 0.000432 0.000208 0.001408 0.001172 0.000252 0.000094 0.000055 0.000975 0.000311 0.000170 0.001272

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.69 -4.51 -3.66 -0.50 -0.14 0.80 -2.43 -3.96 -4.18 -2.78 -3.54 -4.96 -3.48 -1.78 -0.41
Bud Neck -4.34 -10.44 -9.16 -5.89 2.18 -2.41 -9.97 -6.97 -6.46 -0.91 -4.36 -14.19 -10.84 -8.66 2.63
Bud Site -3.93 -6.09 -9.23 -2.84 -3.95 -3.56 -6.80 -8.32 -6.36 -6.22 -5.04 -8.90 -11.46 -3.44 -5.10
Cell Periphery -8.63 -11.47 -10.19 2.30 6.33 5.06 -6.56 -5.96 -7.08 -3.06 -3.86 -13.02 -8.90 -5.25 6.05
Cytoplasm 13.69 45.79 40.41 18.39 -7.33 3.72 24.79 14.16 12.81 -0.43 16.10 50.85 39.50 24.94 -8.01
Cytoplasmic Foci -5.25 -8.08 -3.79 2.94 5.81 -1.19 -6.59 -8.97 -8.67 -5.56 -4.73 -10.35 -7.20 -2.34 4.45
Eisosomes 0.73 -6.27 -7.04 -6.60 0.11 -1.02 -6.55 -4.72 -3.33 -2.93 -0.88 -9.04 -8.38 -1.47 -0.50
Endoplasmic Reticulum -0.97 0.98 3.74 3.70 4.87 1.50 1.21 3.48 2.50 1.05 0.28 1.60 5.40 4.67 2.86
Endosome -1.43 -2.55 -1.81 0.25 0.86 -2.90 -1.15 -0.70 2.69 1.08 -3.27 -2.20 -1.82 1.67 1.15
Golgi -2.54 -0.64 2.69 4.93 1.32 0.17 -0.11 4.25 1.88 1.10 -1.09 -0.59 4.62 3.41 1.72
Lipid Particles -5.02 -7.08 -9.58 -3.21 1.14 1.01 -6.10 -2.53 -2.59 -1.67 -3.64 -9.04 -7.12 -5.58 0.12
Mitochondria 3.57 -0.19 2.78 -1.66 4.18 -1.53 -6.68 2.45 2.85 8.06 2.81 -2.57 1.38 -1.65 4.29
Mitotic Spindle 0.89 -3.21 -5.84 -6.10 -4.17 -3.46 -5.90 -2.41 1.25 0.76 -2.71 -4.37 -6.06 -4.95 -4.43
None -2.30 -2.67 1.23 3.62 5.39 -0.61 0.30 -0.11 0.19 -0.26 -3.21 -1.36 3.02 5.87 4.86
Nuclear Periphery -8.67 -21.40 -15.93 -12.57 2.53 0.09 -8.35 -4.79 -8.09 4.25 -3.02 -20.40 -13.81 -13.88 2.97
Nuclear Periphery Foci -7.29 -8.53 -6.36 3.77 1.68 4.35 0.91 -1.45 -5.14 -2.26 -3.82 -5.47 -3.01 0.73 1.68
Nucleolus -1.11 -2.01 -2.24 1.01 -1.08 -3.37 -4.61 -0.24 4.50 7.35 -1.32 -4.77 -3.37 0.91 -0.50
Nucleus -12.13 -40.16 -25.28 -8.76 14.97 -0.95 -16.33 -1.03 -0.64 8.60 -12.45 -41.31 -21.28 -10.69 16.31
Peroxisomes 1.45 8.04 -0.30 -0.92 -1.45 -4.20 -5.70 -4.17 0.40 -2.98 -1.10 6.42 -0.84 -0.46 -1.52
Vacuole -1.72 -13.13 -24.62 -21.64 -12.54 -6.92 -12.32 -11.15 -10.12 -5.48 -6.85 -17.80 -27.09 -23.32 -11.38
Vacuole Periphery 0.97 1.57 1.20 0.43 -0.62 1.26 1.44 1.50 3.21 2.72 1.73 2.13 1.66 0.12 -0.67
Standard name
Human Ortholog
Description Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ura5

Ura5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ura5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available