Standard name | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 28 | 20 | 47 | 43 | 32 | 34 | – | 34 | 18 | 49 | 34 | 35 | 21 | 25 | 28 | 15 | 7 | 5 | 7 | 7 | 8 | 5 | 15 | 14 |
Bud | 6 | 3 | 25 | 5 | 12 | 3 | – | 5 | 8 | 16 | 9 | 9 | 2 | 1 | 0 | 2 | 1 | 3 | 0 | 1 | 0 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 1 | 3 | 1 | 1 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 11 | 18 | 21 | 34 | 28 | 29 | – | 30 | 43 | 57 | 43 | 29 | 61 | 64 | 71 | 32 | 17 | 7 | 2 | 4 | 12 | 5 | 15 | 23 |
Cytoplasm | 64 | 33 | 56 | 33 | 43 | 41 | – | 75 | 51 | 81 | 45 | 56 | 23 | 24 | 30 | 22 | 18 | 15 | 11 | 4 | 2 | 4 | 6 | 6 |
Endoplasmic Reticulum | 209 | 222 | 183 | 207 | 131 | 205 | – | 184 | 148 | 228 | 176 | 177 | 274 | 223 | 247 | 0 | 2 | 1 | 323 | 266 | 347 | 160 | 232 | 227 |
Endosome | 1 | 0 | 0 | 1 | 13 | 1 | – | 4 | 2 | 2 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 3 | 4 |
Golgi | 1 | 4 | 0 | 3 | 15 | 1 | – | 2 | 3 | 1 | 3 | 1 | 3 | 4 | 0 | 1 | 0 | 0 | 4 | 1 | 3 | 0 | 4 | 3 |
Mitochondria | 9 | 2 | 90 | 140 | 56 | 99 | – | 108 | 120 | 138 | 122 | 120 | 6 | 4 | 2 | 5 | 4 | 0 | 7 | 2 | 9 | 3 | 2 | 4 |
Nucleus | 3 | 1 | 2 | 1 | 1 | 0 | – | 3 | 2 | 7 | 2 | 8 | 0 | 0 | 3 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 5 | 6 | 11 | 7 | 14 | – | 16 | 20 | 19 | 21 | 16 | 2 | 2 | 4 | 0 | 0 | 0 | 2 | 2 | 5 | 2 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | – | 5 | 2 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 3 | 0 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 52 | 21 | 29 | 10 | 53 | 32 | – | 66 | 43 | 68 | 13 | 31 | 4 | 2 | 7 | 2 | 3 | 3 | 13 | 6 | 3 | 3 | 8 | 12 |
Unique Cell Count | 324 | 280 | 348 | 325 | 296 | 342 | 418 | 345 | 507 | 339 | 366 | 333 | 283 | 314 | 68 | 45 | 29 | 384 | 302 | 401 | 193 | 292 | 306 | |
Labelled Cell Count | 384 | 329 | 459 | 489 | 396 | 459 | 533 | 460 | 668 | 470 | 488 | 397 | 350 | 393 | 80 | 53 | 37 | 384 | 302 | 401 | 193 | 292 | 306 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.2 | 11.4 | 7.6 | 9.2 | 10.7 | 9.6 | – | 9.6 | 9.5 | 9.7 | 9.6 | 9.6 | 11.2 | 11.0 | 10.9 | 11.0 | 10.4 | 10.0 | 10.6 | 10.8 | 10.7 |
Std Deviation (1e-4) | 1.4 | 1.8 | 1.6 | 1.5 | 1.8 | 2.0 | – | 1.7 | 1.9 | 1.7 | 1.9 | 2.2 | 1.5 | 1.7 | 1.6 | 2.0 | 1.5 | 1.6 | 1.6 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.29 | 0.5 | 0.35 | – | 0.34 | 0.33 | 0.37 | 0.34 | 0.35 | 0.57 | 0.54 | 0.53 | 0.54 | 0.46 | 0.4 | 0.49 | 0.51 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.1 | -1.0 | -1.5 | – | -2.4 | -3.7 | -1.7 | -1.4 | -1.7 | -3.1 | -1.8 | -1.9 | 1.8 | 0.4 | 0 |
Bud | -3.5 | -1.7 | -4.2 | – | -4.3 | -3.0 | -2.7 | -2.7 | -3.0 | -4.4 | -4.3 | -4.8 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.1 | 1.6 | 1.2 | – | 0.6 | 2.9 | 2.6 | 3.0 | 1.0 | 4.9 | 6.1 | 6.2 | 9.3 | 0 | 0 |
Cytoplasm | -2.3 | -0.5 | -1.6 | – | 0.7 | -0.5 | 0 | -1.0 | -0.3 | -3.7 | -2.9 | -2.5 | 3.1 | 3.9 | 4.7 |
Endoplasmic Reticulum | 2.9 | -2.1 | 1.9 | – | -2.4 | -2.6 | -2.2 | -0.2 | -1.1 | 8.2 | 6.8 | 7.0 | -8.0 | -6.1 | -5.1 |
Endosome | 0 | 3.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 4.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.7 | -2.1 | 0.9 | – | 0 | 2.6 | 0.4 | 2.9 | 2.0 | -9.0 | -8.6 | -9.4 | -3.3 | -2.5 | -3.1 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1.4 | 0.6 | 1.9 | – | 1.7 | 2.8 | 1.7 | 3.0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.9 | 3.6 | 0.5 | – | 3.1 | 1.8 | 2.3 | -2.5 | 0.1 | -4.3 | -4.4 | -3.5 | -1.5 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.8569 | 6.6347 | 6.3938 | 5.9513 | 4.7327 | 6.0878 | 5.4483 | 6.27 | 5.8439 | 5.9682 | 5.8541 | 5.9662 | 7.5681 | 8.7457 | 8.5461 | 9.0756 | 8.6249 | 8.3002 |
Actin | 0.0197 | 0.0073 | 0.0205 | 0.0033 | 0.0305 | 0.0006 | 0.0259 | 0.0175 | 0.0227 | 0.0003 | 0.0002 | 0.007 | 0.0288 | 0.0046 | 0.0079 | 0.0464 | 0.0019 | 0.0005 |
Bud | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 |
Bud Neck | 0.0008 | 0.002 | 0.0031 | 0.0008 | 0.0003 | 0.0003 | 0.0011 | 0.0008 | 0.0024 | 0.0001 | 0.0002 | 0.0014 | 0.0013 | 0.0008 | 0.0014 | 0.0001 | 0.0003 | 0.001 |
Bud Periphery | 0.0013 | 0.0004 | 0.0006 | 0.0004 | 0.0004 | 0 | 0.001 | 0.0007 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0003 | 0.0008 | 0.0003 | 0.0001 | 0.0001 |
Bud Site | 0.0078 | 0.0124 | 0.0013 | 0.0012 | 0.003 | 0 | 0.0028 | 0.0025 | 0.003 | 0 | 0.0001 | 0.0002 | 0.0021 | 0.0076 | 0.0087 | 0.0002 | 0.0001 | 0 |
Cell Periphery | 0.0025 | 0.0246 | 0.0281 | 0.002 | 0.0003 | 0.0012 | 0.0046 | 0.0238 | 0.0096 | 0.0005 | 0.0004 | 0.0011 | 0.0028 | 0.0179 | 0.0184 | 0.0002 | 0.001 | 0.0015 |
Cytoplasm | 0.0323 | 0.1122 | 0.0617 | 0.0197 | 0.0092 | 0.0268 | 0.0554 | 0.0878 | 0.0594 | 0.002 | 0.0031 | 0.0356 | 0.0352 | 0.1236 | 0.0821 | 0.003 | 0.014 | 0.0396 |
Cytoplasmic Foci | 0.0095 | 0.0046 | 0.0069 | 0.0112 | 0.0173 | 0.001 | 0.013 | 0.0059 | 0.0132 | 0.0002 | 0.001 | 0.0066 | 0.0098 | 0.0042 | 0.0055 | 0.0012 | 0.0054 | 0.0008 |
Eisosomes | 0.0003 | 0.0009 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0006 | 0.0015 | 0.0008 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.8041 | 0.7738 | 0.816 | 0.8536 | 0.8164 | 0.8674 | 0.7198 | 0.7602 | 0.7914 | 0.9263 | 0.887 | 0.84 | 0.7578 | 0.7574 | 0.8245 | 0.8826 | 0.888 | 0.8503 |
Endosome | 0.0261 | 0.0094 | 0.0088 | 0.0325 | 0.0158 | 0.0042 | 0.0481 | 0.0152 | 0.0239 | 0.0093 | 0.016 | 0.0186 | 0.0323 | 0.0073 | 0.0102 | 0.0037 | 0.0073 | 0.0067 |
Golgi | 0.0078 | 0.0059 | 0.0126 | 0.0137 | 0.0126 | 0.0012 | 0.0141 | 0.0122 | 0.0142 | 0.0042 | 0.0047 | 0.0082 | 0.011 | 0.006 | 0.0079 | 0.0087 | 0.0044 | 0.0007 |
Lipid Particles | 0.0078 | 0.0286 | 0.0208 | 0.0112 | 0.0452 | 0.0015 | 0.0177 | 0.0326 | 0.0218 | 0.0012 | 0.0107 | 0.0081 | 0.0255 | 0.0335 | 0.0156 | 0.0004 | 0.0211 | 0.0015 |
Mitochondria | 0.0083 | 0.0007 | 0.0011 | 0.0218 | 0.0075 | 0.0002 | 0.0237 | 0.0027 | 0.0118 | 0.0315 | 0.0107 | 0.004 | 0.0325 | 0.0006 | 0.0012 | 0.0004 | 0.0016 | 0.0003 |
None | 0.0043 | 0.0013 | 0.0003 | 0.0009 | 0.0007 | 0.0003 | 0.0078 | 0.0033 | 0.0029 | 0.0002 | 0.0001 | 0.004 | 0.0033 | 0.0009 | 0.0016 | 0.0001 | 0.0003 | 0.0006 |
Nuclear Periphery | 0.0346 | 0.0092 | 0.0079 | 0.0091 | 0.0041 | 0.0839 | 0.0376 | 0.018 | 0.0074 | 0.0117 | 0.0564 | 0.0526 | 0.0222 | 0.0272 | 0.0066 | 0.05 | 0.0215 | 0.086 |
Nucleolus | 0.0003 | 0.0001 | 0.0004 | 0.0009 | 0.0002 | 0 | 0.0008 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0159 | 0.0008 | 0.0008 | 0.0021 | 0.0004 | 0.007 | 0.0065 | 0.0027 | 0.0029 | 0.0003 | 0.0012 | 0.0021 | 0.0038 | 0.0018 | 0.0007 | 0.0008 | 0.0084 | 0.0047 |
Peroxisomes | 0.0057 | 0.0001 | 0.0033 | 0.0029 | 0.0326 | 0 | 0.0026 | 0.0002 | 0.0058 | 0 | 0.0003 | 0.0017 | 0.022 | 0.0001 | 0.001 | 0.0001 | 0.0186 | 0 |
Punctate Nuclear | 0.0024 | 0.0004 | 0.0021 | 0.0077 | 0.0011 | 0.0001 | 0.0029 | 0.0004 | 0.0008 | 0 | 0.0001 | 0.0017 | 0.0019 | 0.0002 | 0.0009 | 0.0001 | 0.0012 | 0.0001 |
Vacuole | 0.0045 | 0.0046 | 0.0028 | 0.0034 | 0.0015 | 0.003 | 0.0096 | 0.0105 | 0.004 | 0.0028 | 0.0044 | 0.0032 | 0.0044 | 0.0045 | 0.0035 | 0.001 | 0.0043 | 0.003 |
Vacuole Periphery | 0.0032 | 0.0006 | 0.0003 | 0.0012 | 0.0004 | 0.0011 | 0.004 | 0.0011 | 0.0009 | 0.0088 | 0.0032 | 0.0035 | 0.0018 | 0.0013 | 0.0005 | 0.0004 | 0.0006 | 0.0023 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 52.9883 | 61.3002 | 37.217 | 44.525 | 38.3921 | 37.3828 | 52.12 | 38.9805 | 50.6206 | 49.0511 |
Translational Efficiency | 1.1648 | 0.7286 | 1.228 | 1.4317 | 1.4916 | 1.8948 | 0.9967 | 1.9575 | 1.2837 | 0.8688 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
241 | 1399 | 1147 | 127 | 1576 | 1022 | 244 | 1291 | 1817 | 2421 | 1391 | 1418 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 816.86 | 995.15 | 1189.81 | 1264.52 | 958.03 | 1034.17 | 1390.45 | 1083.25 | 939.31 | 1011.62 | 1225.00 | 1099.49 |
Standard Deviation | 196.65 | 153.68 | 176.37 | 167.93 | 132.38 | 146.27 | 166.59 | 152.52 | 150.41 | 151.82 | 190.64 | 162.43 |
Intensity Change Log 2 | — | 0.284825 | 0.542570 | 0.630429 | — | 0.110331 | 0.537409 | 0.177223 | — | 0.193267 | 0.539787 | 0.403561 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003728 | 0.005110 | 0.002476 | 0.014557 | 0.001579 | 0.002920 | 0.010276 | 0.016114 | 0.001864 | 0.004186 | 0.003844 | 0.015974 |
Bud Neck | 0.006491 | 0.001763 | 0.003845 | 0.006665 | 0.000746 | 0.001540 | 0.001506 | 0.014663 | 0.001508 | 0.001669 | 0.003435 | 0.013947 |
Bud Site | 0.009267 | 0.015681 | 0.019490 | 0.025738 | 0.002667 | 0.006816 | 0.012483 | 0.052827 | 0.003542 | 0.011939 | 0.018261 | 0.050401 |
Cell Periphery | 0.013390 | 0.006257 | 0.004971 | 0.024565 | 0.007149 | 0.005980 | 0.010339 | 0.003424 | 0.007976 | 0.006140 | 0.005912 | 0.005317 |
Cytoplasm | 0.076857 | 0.063078 | 0.045013 | 0.031625 | 0.045078 | 0.077848 | 0.013144 | 0.150513* | 0.049293 | 0.069313 | 0.039422 | 0.139865* |
Cytoplasmic Foci | 0.047926 | 0.026683 | 0.005078 | 0.012236 | 0.006971 | 0.020599 | 0.003368 | 0.087418 | 0.012403 | 0.024115 | 0.004779 | 0.080684 |
Eisosomes | 0.001415 | 0.000990 | 0.000164 | 0.001285 | 0.001077 | 0.001490 | 0.000510 | 0.001785 | 0.001122 | 0.001201 | 0.000225 | 0.001740 |
Endoplasmic Reticulum | 0.633405* | 0.800940* | 0.841204* | 0.760378* | 0.873863* | 0.799355* | 0.895122* | 0.340848* | 0.841969* | 0.800271* | 0.850662* | 0.378423* |
Endosome | 0.024408 | 0.010155 | 0.012941 | 0.012865 | 0.004435 | 0.008726 | 0.009545 | 0.043334 | 0.007084 | 0.009552 | 0.012346 | 0.040605 |
Golgi | 0.033537 | 0.016379 | 0.003310 | 0.003672 | 0.011781 | 0.018223 | 0.002580 | 0.023581 | 0.014667 | 0.017157 | 0.003182 | 0.021798 |
Lipid Particles | 0.066780 | 0.016392 | 0.005200 | 0.040313 | 0.015454 | 0.020612 | 0.018664 | 0.086855 | 0.022261 | 0.018173 | 0.007561 | 0.082687 |
Mitochondria | 0.005162 | 0.007436 | 0.000780 | 0.000059 | 0.003223 | 0.006782 | 0.000477 | 0.005225 | 0.003480 | 0.007160 | 0.000727 | 0.004763 |
Mitotic Spindle | 0.000185 | 0.000403 | 0.000590 | 0.002134 | 0.000107 | 0.000379 | 0.000627 | 0.010496 | 0.000118 | 0.000392 | 0.000597 | 0.009747 |
None | 0.014476 | 0.002497 | 0.001669 | 0.000043 | 0.002907 | 0.003338 | 0.000085 | 0.001941 | 0.004442 | 0.002852 | 0.001391 | 0.001771 |
Nuclear Periphery | 0.004469 | 0.001326 | 0.005869 | 0.000320 | 0.003025 | 0.001588 | 0.000676 | 0.002315 | 0.003216 | 0.001436 | 0.004958 | 0.002136 |
Nuclear Periphery Foci | 0.014859 | 0.006896 | 0.033316 | 0.010274 | 0.008969 | 0.005852 | 0.011275 | 0.025456 | 0.009750 | 0.006456 | 0.029450 | 0.024096 |
Nucleolus | 0.001300 | 0.000482 | 0.000029 | 0.000017 | 0.000107 | 0.000040 | 0.000004 | 0.000250 | 0.000265 | 0.000295 | 0.000025 | 0.000230 |
Nucleus | 0.018309 | 0.002054 | 0.002600 | 0.002844 | 0.001154 | 0.002567 | 0.000677 | 0.018898 | 0.003430 | 0.002271 | 0.002263 | 0.017460 |
Peroxisomes | 0.008183 | 0.006506 | 0.000240 | 0.001075 | 0.006774 | 0.007424 | 0.000696 | 0.007551 | 0.006961 | 0.006893 | 0.000320 | 0.006971 |
Vacuole | 0.011537 | 0.005884 | 0.010304 | 0.049026 | 0.002100 | 0.005857 | 0.007716 | 0.103004 | 0.003352 | 0.005873 | 0.009850 | 0.098170 |
Vacuole Periphery | 0.004315 | 0.003088 | 0.000913 | 0.000310 | 0.000837 | 0.002066 | 0.000230 | 0.003502 | 0.001298 | 0.002657 | 0.000793 | 0.003216 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.70 | 0.66 | -1.97 | -1.79 | -2.32 | -1.57 | -2.33 | -9.04 | -7.82 | -1.38 | -3.16 | -2.29 | -9.11 | -7.50 | -7.34 |
Bud Neck | 1.71 | 0.82 | -0.08 | -3.08 | -1.50 | -2.32 | -1.99 | -10.23 | -9.45 | -9.27 | -0.37 | -3.39 | -9.58 | -9.74 | -7.59 |
Bud Site | -2.08 | -3.09 | -2.38 | -1.50 | -0.87 | -3.02 | -2.59 | -13.62 | -11.92 | -7.52 | -6.26 | -6.99 | -13.69 | -10.71 | -8.00 |
Cell Periphery | 1.48 | 1.72 | -1.27 | -2.54 | -2.70 | 0.99 | -1.46 | 3.88 | 3.00 | 3.47 | 1.60 | 1.52 | 2.23 | 1.01 | 0.65 |
Cytoplasm | 1.55 | 3.41 | 4.04 | 4.05 | 1.69 | -6.85 | 9.12 | -18.29 | -10.88 | -23.29 | -5.92 | 2.50 | -16.67 | -12.91 | -17.52 |
Cytoplasmic Foci | 3.01 | 6.28 | 4.57 | 3.26 | -1.95 | -6.17 | 2.30 | -21.23 | -15.89 | -21.55 | -6.06 | 5.39 | -18.82 | -15.18 | -21.69 |
Eisosomes | 0.74 | 2.23 | 0.22 | -0.71 | -2.94 | -1.51 | 2.82 | -2.66 | -0.86 | -5.45 | -0.41 | 5.48 | -2.43 | -2.36 | -8.08 |
Endoplasmic Reticulum | -6.41 | -7.82 | -3.06 | 1.70 | 2.94 | 7.19 | -1.31 | 48.23* | 35.37* | 33.62* | 5.01 | -0.68 | 40.82 | 37.91 | 40.26* |
Endosome | 4.27 | 3.35 | 2.60 | -1.03 | -0.04 | -3.91 | -2.01 | -17.15 | -13.92 | -9.81 | -3.06 | -5.21 | -15.66 | -14.36 | -12.46 |
Golgi | 3.18 | 5.76 | 5.66 | 8.60 | -0.34 | -3.10 | 6.94 | -5.85 | -1.92 | -12.01 | -1.58 | 9.15 | -3.47 | -2.20 | -12.43 |
Lipid Particles | 5.56 | 6.82 | 2.15 | -2.82 | -4.14 | -2.50 | -0.65 | -16.82 | -14.97 | -9.57 | 2.17 | 7.28 | -14.57 | -16.40 | -18.84 |
Mitochondria | -1.49 | 3.83 | 4.54 | 7.12 | 3.92 | -2.54 | 3.88 | -1.64 | 1.15 | -4.91 | -3.72 | 4.48 | -1.10 | 2.27 | -4.64 |
Mitotic Spindle | -1.17 | -2.20 | -1.27 | -1.13 | -1.00 | -1.28 | -1.33 | -5.75 | -5.58 | -5.30 | -1.91 | -2.83 | -5.83 | -5.66 | -5.51 |
None | 2.03 | 2.17 | 2.46 | 4.04 | 3.03 | -0.30 | 3.74 | 0.89 | 0.98 | -1.99 | 1.33 | 2.75 | 2.10 | 1.12 | -0.34 |
Nuclear Periphery | 2.17 | -0.79 | 2.87 | 3.39 | 6.19 | 1.80 | 3.36 | 0.89 | -1.26 | -3.75 | 2.71 | -1.82 | 1.51 | -1.64 | 3.44 |
Nuclear Periphery Foci | 2.29 | -4.25 | 0.94 | -1.69 | 6.24 | 1.88 | -0.81 | -7.01 | -8.53 | -4.09 | 2.43 | -7.56 | -6.65 | -8.93 | 1.61 |
Nucleolus | 1.41 | 2.52 | 2.55 | 1.54 | 1.16 | 0.98 | 1.48 | -2.02 | -6.62 | -7.73 | -0.13 | 2.65 | 0.27 | 0.30 | -6.92 |
Nucleus | 3.17 | 3.00 | 2.86 | -1.16 | -0.39 | -3.97 | 1.94 | -13.19 | -11.98 | -13.48 | 1.59 | 1.14 | -10.13 | -12.30 | -11.70 |
Peroxisomes | 1.13 | 6.13 | 5.31 | 6.87 | -2.45 | -0.57 | 8.47 | -0.66 | -0.07 | -7.26 | 0.08 | 10.95 | 0.02 | -0.04 | -8.03 |
Vacuole | 2.33 | 0.32 | -3.41 | -3.89 | -3.51 | -4.84 | -3.82 | -22.89 | -21.88 | -20.66 | -4.11 | -7.03 | -22.78 | -22.18 | -20.93 |
Vacuole Periphery | 1.05 | 3.16 | 3.75 | 5.93 | 4.01 | -2.87 | 3.46 | -5.41 | -2.17 | -6.83 | -3.70 | 2.33 | -4.01 | -0.86 | -5.46 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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