Standard name | |
---|---|
Human Ortholog | |
Description | Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; contributes to survival to acute lethal heat stress; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 2 | 1 | 5 | 8 | 11 | 9 | 7 | 13 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 1 | 0 | 10 | 3 | 4 | 3 | 6 | 13 | 13 | 5 | 5 | 7 | 2 | 1 | 3 | 0 | 0 | 0 | 2 | 1 | 2 | 3 | 2 | 4 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 0 | 3 | 2 | 9 | 12 | 13 | 22 | 22 | 27 | 26 | 24 | 1 | 3 | 2 | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 131 | 149 | 284 | 246 | 323 | 223 | 282 | 268 | 268 | 170 | 138 | 160 | 219 | 208 | 204 | 59 | 53 | 17 | 133 | 150 | 277 | 122 | 201 | 216 |
Endoplasmic Reticulum | 1 | 1 | 2 | 4 | 2 | 5 | 20 | 19 | 21 | 26 | 19 | 17 | 5 | 4 | 6 | 4 | 2 | 1 | 2 | 0 | 4 | 3 | 13 | 18 |
Endosome | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 4 | 10 | 45 | 40 | 77 | 95 | 104 | 104 | 104 | 75 | 107 | 1 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 13 | 0 | 0 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 4 | 2 | 0 | 7 | 5 | 17 | 55 | 44 | 37 | 22 | 34 | 0 | 1 | 2 | 2 | 12 | 2 | 0 | 5 | 5 | 2 | 7 | 4 |
Unique Cell Count | 133 | 156 | 293 | 256 | 344 | 273 | 380 | 425 | 418 | 309 | 245 | 289 | 224 | 212 | 213 | 62 | 69 | 19 | 145 | 163 | 312 | 135 | 233 | 252 |
Labelled Cell Count | 143 | 161 | 311 | 301 | 387 | 327 | 441 | 499 | 493 | 380 | 294 | 366 | 228 | 218 | 223 | 70 | 80 | 21 | 145 | 163 | 312 | 135 | 233 | 252 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.3 | 8.9 | 5.7 | 4.8 | 6.4 | 6.9 | 7.9 | 9.0 | 9.0 | 10.2 | 10.0 | 11.8 | 13.4 | 18.4 | 19.3 | 28.6 | 31.7 | 31.1 | 13.3 | 15.3 | 13.0 |
Std Deviation (1e-4) | 3.1 | 3.1 | 2.2 | 1.4 | 2.1 | 2.1 | 2.5 | 3.0 | 3.0 | 4.3 | 4.2 | 5.8 | 5.0 | 8.2 | 8.4 | 10.1 | 15.3 | 11.4 | 4.3 | 6.5 | 4.3 |
Intensity Change (Log2) | – | – | – | -0.26 | 0.16 | 0.26 | 0.47 | 0.65 | 0.65 | 0.84 | 0.81 | 1.04 | 1.23 | 1.69 | 1.75 | 2.32 | 2.47 | 2.44 | 1.22 | 1.42 | 1.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -1.9 | -1.8 | -1.5 | -0.3 | -0.2 | -1.4 | -1.0 | -0.7 | -1.9 | 0 | -1.4 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.5 | 2.5 | 2.0 | 3.0 | 3.0 | 4.3 | 4.9 | 4.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -1.8 | -5.9 | -8.0 | -10.6 | -10.3 | -11.9 | -11.4 | -11.8 | 0.6 | 0.8 | -0.7 | -0.7 | -5.9 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.3 | 3.0 | 3.2 | 4.5 | 4.2 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 3.8 | 8.2 | 7.7 | 7.6 | 7.7 | 9.5 | 8.6 | 10.1 | 0 | 0 | -1.0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.9 | 6.0 | 5.3 | 5.6 | 4.6 | 5.5 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.5751 | 12.0254 | 11.4497 | 10.2003 | 8.25 | 11.2524 | 17.2445 | 23.0095 | 18.6384 | 16.1111 | 15.2346 | 21.2014 | 21.0353 | 24.3405 | 23.9012 | 17.9262 | 25.0284 | 21.136 |
Actin | 0.0533 | 0.005 | 0.0161 | 0.0066 | 0.0214 | 0.003 | 0.0331 | 0.0004 | 0.03 | 0.0068 | 0.062 | 0.0164 | 0.0161 | 0.0046 | 0.0038 | 0.0028 | 0.0002 | 0.0012 |
Bud | 0.0032 | 0.0014 | 0.0014 | 0.004 | 0.0383 | 0.0025 | 0.0015 | 0.0008 | 0.0007 | 0.0014 | 0.0052 | 0.0005 | 0.0014 | 0.0009 | 0.0026 | 0.0101 | 0.0003 | 0.0008 |
Bud Neck | 0.0042 | 0.0006 | 0.0008 | 0.0014 | 0.0053 | 0.0016 | 0.0011 | 0.0003 | 0.0008 | 0.0018 | 0.002 | 0.0014 | 0.0005 | 0.0003 | 0.0007 | 0.0008 | 0.0004 | 0.001 |
Bud Periphery | 0.0052 | 0.0011 | 0.0008 | 0.0042 | 0.0288 | 0.0067 | 0.0015 | 0.0003 | 0.0005 | 0.0007 | 0.004 | 0.0004 | 0.0011 | 0.0003 | 0.0012 | 0.0265 | 0 | 0.0006 |
Bud Site | 0.0096 | 0.0036 | 0.0017 | 0.0026 | 0.0071 | 0.0006 | 0.0035 | 0.0019 | 0.0018 | 0.0203 | 0.0039 | 0.0007 | 0.001 | 0.0034 | 0.0033 | 0.0011 | 0.0001 | 0.0001 |
Cell Periphery | 0.0023 | 0.0013 | 0.0007 | 0.0015 | 0.0008 | 0.0012 | 0.0007 | 0.0008 | 0.0002 | 0.0002 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0017 | 0.0001 | 0.0002 |
Cytoplasm | 0.5547 | 0.9177 | 0.8375 | 0.7403 | 0.6896 | 0.8174 | 0.739 | 0.966 | 0.8394 | 0.8949 | 0.6921 | 0.8506 | 0.8015 | 0.935 | 0.891 | 0.8474 | 0.9056 | 0.9142 |
Cytoplasmic Foci | 0.0447 | 0.0051 | 0.0116 | 0.0062 | 0.0331 | 0.0093 | 0.0152 | 0.0031 | 0.0156 | 0.0104 | 0.0411 | 0.0162 | 0.0131 | 0.0069 | 0.0127 | 0.0129 | 0.0087 | 0.0096 |
Eisosomes | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.07 | 0.0247 | 0.0139 | 0.0397 | 0.0086 | 0.0586 | 0.0518 | 0.011 | 0.0115 | 0.0029 | 0.0081 | 0.0453 | 0.046 | 0.0064 | 0.0208 | 0.0256 | 0.041 | 0.0182 |
Endosome | 0.069 | 0.0078 | 0.03 | 0.0427 | 0.0374 | 0.0308 | 0.0402 | 0.0042 | 0.0264 | 0.0227 | 0.0317 | 0.0306 | 0.0347 | 0.0124 | 0.0354 | 0.0346 | 0.0218 | 0.0308 |
Golgi | 0.0361 | 0.0026 | 0.0105 | 0.0027 | 0.0109 | 0.006 | 0.0055 | 0.0005 | 0.0096 | 0.0038 | 0.0294 | 0.0057 | 0.0151 | 0.0045 | 0.0025 | 0.0023 | 0.0006 | 0.0038 |
Lipid Particles | 0.0201 | 0.0004 | 0.0028 | 0.001 | 0.0041 | 0.0082 | 0.0122 | 0.0001 | 0.0054 | 0.0019 | 0.0143 | 0.0049 | 0.0028 | 0.0006 | 0.0003 | 0.0002 | 0 | 0.0001 |
Mitochondria | 0.0466 | 0.0012 | 0.0036 | 0.0111 | 0.0141 | 0.0188 | 0.0069 | 0.0001 | 0.0149 | 0.0008 | 0.0435 | 0.0025 | 0.0361 | 0.0101 | 0.0007 | 0.0019 | 0.0002 | 0.0095 |
None | 0.0056 | 0.0079 | 0.0023 | 0.0021 | 0.0027 | 0.0059 | 0.0094 | 0.0015 | 0.0016 | 0.0007 | 0.0173 | 0.0026 | 0.001 | 0.0005 | 0.0007 | 0.0007 | 0.0003 | 0.0004 |
Nuclear Periphery | 0.0095 | 0.002 | 0.0096 | 0.0203 | 0.0281 | 0.0044 | 0.0163 | 0.0009 | 0.0112 | 0.002 | 0.004 | 0.0056 | 0.0052 | 0.0019 | 0.0019 | 0.0072 | 0.0018 | 0.0013 |
Nucleolus | 0.0012 | 0.0001 | 0.0002 | 0.0011 | 0.0004 | 0.0001 | 0.0024 | 0 | 0.0005 | 0.0002 | 0.0008 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0054 | 0.0017 | 0.0054 | 0.0151 | 0.005 | 0.0023 | 0.0073 | 0.001 | 0.0048 | 0.0022 | 0.0023 | 0.0038 | 0.0025 | 0.0011 | 0.0012 | 0.0032 | 0.001 | 0.0009 |
Peroxisomes | 0.0107 | 0.0003 | 0.0008 | 0.0002 | 0.0325 | 0.0004 | 0.004 | 0 | 0.0044 | 0.0023 | 0.0108 | 0.0016 | 0.0016 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0001 |
Punctate Nuclear | 0.0205 | 0.0001 | 0.0012 | 0.0005 | 0.0007 | 0.0003 | 0.0073 | 0.0001 | 0.0042 | 0.0007 | 0.0029 | 0.0012 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Vacuole | 0.0235 | 0.0141 | 0.0404 | 0.0843 | 0.0168 | 0.0177 | 0.0351 | 0.0065 | 0.0136 | 0.0186 | 0.0194 | 0.0074 | 0.0134 | 0.0089 | 0.0172 | 0.0165 | 0.0147 | 0.0051 |
Vacuole Periphery | 0.0042 | 0.0011 | 0.0085 | 0.0122 | 0.0141 | 0.0041 | 0.0056 | 0.0006 | 0.0026 | 0.0046 | 0.0042 | 0.0023 | 0.0056 | 0.0015 | 0.0033 | 0.0043 | 0.0031 | 0.0021 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.7214 | 9.8727 | 16.206 | 63.6219 | 42.9574 | 39.4691 | 29.6444 | 48.6929 | 86.9624 | 65.1924 |
Translational Efficiency | 0.7418 | 0.6222 | 0.85 | 0.8972 | 0.9001 | 0.8454 | 0.6936 | 0.9416 | 1.0715 | 0.8308 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1655 | 1458 | 1189 | 1491 | 1582 | 1844 | 1775 | 527 | 3237 | 3302 | 2964 | 2018 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 834.68 | 740.44 | 5505.17 | 1884.30 | 840.69 | 915.79 | 2368.40 | 1698.66 | 837.62 | 838.36 | 3626.71 | 1835.82 |
Standard Deviation | 158.39 | 126.22 | 1396.82 | 631.83 | 237.44 | 200.96 | 611.04 | 669.82 | 200.97 | 192.80 | 1835.74 | 647.13 |
Intensity Change Log 2 | — | -0.172840 | 2.721492 | 1.174734 | — | 0.123443 | 1.494267 | 1.014751 | — | -0.016577 | 2.232538 | 1.096672 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000232 | 0.000398 | 0.000229 | 0.000381 | 0.000155 | 0.000408 | 0.000141 | 0.000369 | 0.000195 | 0.000404 | 0.000176 | 0.000378 |
Bud Neck | 0.004002 | 0.018033 | 0.003252 | 0.002733 | 0.005236 | 0.005724 | 0.001116 | 0.003156 | 0.004605 | 0.011159 | 0.001973 | 0.002843 |
Bud Site | 0.039866 | 0.033284 | 0.004933 | 0.020600 | 0.031037 | 0.038237 | 0.007252 | 0.030332 | 0.035551 | 0.036050 | 0.006322 | 0.023142 |
Cell Periphery | 0.000301 | 0.000110 | 0.023536 | 0.000860 | 0.000313 | 0.000173 | 0.001273 | 0.002890 | 0.000307 | 0.000145 | 0.010204 | 0.001390 |
Cytoplasm | 0.730134* | 0.496263* | 0.610515* | 0.728642* | 0.723573* | 0.682787* | 0.892881* | 0.654885* | 0.726927* | 0.600427* | 0.779611* | 0.709380* |
Cytoplasmic Foci | 0.145134 | 0.226092* | 0.017777 | 0.024421 | 0.123203 | 0.169134 | 0.012293 | 0.042792 | 0.134416 | 0.194284* | 0.014493 | 0.029218 |
Eisosomes | 0.000037 | 0.000068 | 0.000005 | 0.000005 | 0.000029 | 0.000037 | 0.000001 | 0.000008 | 0.000033 | 0.000051 | 0.000003 | 0.000005 |
Endoplasmic Reticulum | 0.003405 | 0.001215 | 0.000646 | 0.001459 | 0.003244 | 0.001661 | 0.000446 | 0.000816 | 0.003326 | 0.001464 | 0.000526 | 0.001291 |
Endosome | 0.011671 | 0.047991 | 0.001024 | 0.009131 | 0.011728 | 0.020658 | 0.001679 | 0.013370 | 0.011699 | 0.032727 | 0.001416 | 0.010238 |
Golgi | 0.009318 | 0.010263 | 0.054976 | 0.010315 | 0.004792 | 0.010813 | 0.009762 | 0.010429 | 0.007106 | 0.010570 | 0.027900 | 0.010345 |
Lipid Particles | 0.001270 | 0.003977 | 0.015915 | 0.004167 | 0.001668 | 0.001350 | 0.003267 | 0.005254 | 0.001465 | 0.002510 | 0.008341 | 0.004451 |
Mitochondria | 0.002162 | 0.005883 | 0.000285 | 0.002814 | 0.001701 | 0.003698 | 0.000128 | 0.006671 | 0.001936 | 0.004663 | 0.000191 | 0.003822 |
Mitotic Spindle | 0.000834 | 0.002176 | 0.005374 | 0.009036 | 0.002714 | 0.005624 | 0.003756 | 0.009048 | 0.001752 | 0.004102 | 0.004405 | 0.009039 |
None | 0.004015 | 0.002192 | 0.002067 | 0.000908 | 0.004712 | 0.000962 | 0.000788 | 0.000512 | 0.004355 | 0.001505 | 0.001301 | 0.000804 |
Nuclear Periphery | 0.000043 | 0.000187 | 0.003136 | 0.000147 | 0.000162 | 0.000088 | 0.000175 | 0.000156 | 0.000101 | 0.000132 | 0.001363 | 0.000149 |
Nuclear Periphery Foci | 0.000334 | 0.000759 | 0.018374 | 0.002275 | 0.000658 | 0.000375 | 0.003946 | 0.002361 | 0.000493 | 0.000545 | 0.009734 | 0.002298 |
Nucleolus | 0.000144 | 0.001767 | 0.000128 | 0.000249 | 0.000315 | 0.000226 | 0.000066 | 0.000376 | 0.000228 | 0.000907 | 0.000091 | 0.000282 |
Nucleus | 0.005679 | 0.016574 | 0.026007 | 0.012255 | 0.018833 | 0.005852 | 0.010004 | 0.009416 | 0.012107 | 0.010586 | 0.016424 | 0.011513 |
Peroxisomes | 0.000786 | 0.007860 | 0.000016 | 0.001039 | 0.000898 | 0.002724 | 0.000067 | 0.001193 | 0.000840 | 0.004992 | 0.000047 | 0.001079 |
Vacuole | 0.031697 | 0.118236 | 0.140207 | 0.155174 | 0.055962 | 0.043457 | 0.040456 | 0.194187 | 0.043556 | 0.076476 | 0.080471 | 0.165362 |
Vacuole Periphery | 0.008937 | 0.006670 | 0.071597 | 0.013391 | 0.009068 | 0.006012 | 0.010502 | 0.011779 | 0.009001 | 0.006303 | 0.035010 | 0.012970 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.30 | -1.52 | -1.10 | 0.27 | -0.62 | -3.28 | -0.09 | -3.38 | 0.51 | -3.13 | -3.86 | -0.69 | -1.91 | 0.41 | -1.72 |
Bud Neck | -10.62 | -0.70 | 1.09 | 11.11 | 1.95 | -0.88 | 8.22 | 2.38 | 3.77 | -6.06 | -9.83 | 6.40 | 2.72 | 11.68 | -3.61 |
Bud Site | 2.55 | 17.15 | 7.95 | 5.19 | -10.63 | -2.55 | 11.22 | 0.79 | 2.70 | -6.21 | -0.20 | 19.84 | 6.61 | 6.83 | -11.71 |
Cell Periphery | 8.98 | -17.59 | -4.27 | -5.92 | 17.13 | 3.48 | -4.85 | -3.06 | -3.27 | -1.57 | 6.93 | -17.11 | -4.62 | -5.43 | 14.68 |
Cytoplasm | 23.50 | 12.05 | 1.52 | -19.88 | -10.02 | 4.35 | -20.49 | 5.01 | 2.32 | 16.51 | 18.20 | -5.44 | 3.62 | -11.33 | 7.76 |
Cytoplasmic Foci | -12.02 | 28.65 | 28.58 | 36.89 | -0.62 | -7.47 | 26.46 | 16.25 | 23.43 | -7.99 | -13.14 | 39.55 | 33.87 | 44.73 | -7.25 |
Eisosomes | -6.48 | 10.05 | 10.84 | 16.79 | 3.28 | -2.29 | 15.34 | 10.52 | 9.64 | -5.81 | -6.00 | 16.72 | 15.48 | 19.19 | -3.74 |
Endoplasmic Reticulum | 3.13 | 4.07 | 2.88 | 0.09 | -1.47 | 2.61 | 5.18 | 4.64 | 3.07 | -1.74 | 3.97 | 6.40 | 4.41 | 1.25 | -1.88 |
Endosome | -15.70 | 15.15 | 3.51 | 16.97 | -7.69 | -6.29 | 12.33 | 0.22 | 4.44 | -5.44 | -16.06 | 19.01 | 3.19 | 16.74 | -9.03 |
Golgi | -0.83 | -21.32 | 0.71 | 1.23 | 20.14* | -6.09 | -5.42 | -2.51 | 1.23 | 0.68 | -4.77 | -18.83 | -1.70 | 1.90 | 14.43 |
Lipid Particles | -6.47 | -16.26 | -7.44 | -1.00 | 11.94 | 1.55 | -4.95 | -4.28 | -4.61 | -2.16 | -4.81 | -16.07 | -8.36 | -5.35 | 6.82 |
Mitochondria | -3.42 | 3.29 | -0.03 | 2.91 | -2.18 | -2.10 | 2.55 | -1.41 | -0.58 | -2.11 | -3.82 | 4.17 | -1.06 | 1.59 | -3.11 |
Mitotic Spindle | -1.91 | -6.44 | -5.74 | -4.64 | -2.29 | -2.39 | -1.76 | -2.12 | -0.96 | -1.51 | -3.24 | -5.55 | -5.65 | -3.79 | -3.26 |
None | 1.92 | 2.17 | 3.59 | 3.33 | 9.85 | 6.28 | 6.70 | 7.08 | 2.48 | 2.13 | 5.09 | 5.71 | 6.69 | 3.57 | 5.74 |
Nuclear Periphery | -4.43 | -8.53 | -5.26 | 0.18 | 8.18 | 1.53 | -0.78 | -0.14 | -1.86 | 0.68 | -1.09 | -8.51 | -2.61 | -1.62 | 8.05 |
Nuclear Periphery Foci | -2.36 | -18.44 | -7.88 | -4.80 | 15.99 | 1.93 | -9.44 | -4.67 | -5.68 | 3.40 | -0.63 | -20.08 | -8.55 | -7.92 | 14.95 |
Nucleolus | -3.12 | -0.14 | -3.52 | 2.87 | -3.64 | 1.82 | 8.66 | -0.31 | -0.90 | -2.11 | -2.96 | 7.98 | -1.53 | 2.63 | -4.44 |
Nucleus | -8.90 | -13.88 | -10.30 | -0.12 | 7.45 | 11.63 | 6.03 | 2.69 | -5.26 | -1.52 | 1.68 | -8.00 | -4.02 | -5.16 | 3.45 |
Peroxisomes | -10.78 | 15.99 | -0.23 | 9.23 | -2.25 | -4.59 | 12.27 | -0.73 | 4.07 | -6.37 | -11.28 | 19.20 | -0.24 | 8.83 | -3.04 |
Vacuole | -20.21 | -20.67 | -21.62 | -6.82 | -2.05 | 4.21 | 3.17 | -13.48 | -14.79 | -14.45 | -12.64 | -13.39 | -24.29 | -17.78 | -15.04 |
Vacuole Periphery | 2.45 | -19.08 | -1.91 | -4.37 | 18.13* | 2.61 | -1.01 | -0.67 | -2.78 | 0.13 | 3.59 | -15.94 | -2.00 | -5.35 | 14.10 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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