Standard name | |
---|---|
Human Ortholog | |
Description | Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; affects expression of multiple genes via its role in nucleosome assembly; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR3 has a paralog, FPR4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 5 | 10 | 12 | 14 | 13 | 17 | 18 | 0 | 2 | 0 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 1 | 4 | 7 | 8 | 3 | 8 | 1 | 7 | 6 | 4 | 4 | 30 | 42 | 51 | 15 | 15 | 4 | 0 | 0 | 0 | 1 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 3 | 2 | 2 | 2 | 2 | 4 | 1 | 0 | 1 | 1 | 3 | 7 | 1 | 3 | 0 | 0 | 1 | 2 | 2 | 2 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 12 | 8 | 16 | 37 | 57 | 62 | 107 | 92 | 115 | 119 | 149 | 119 | 20 | 5 | 9 | 33 | 23 | 13 | 0 | 0 | 2 | 6 | 9 | 12 |
Nucleus | 145 | 90 | 74 | 10 | 48 | 28 | 45 | 40 | 40 | 38 | 53 | 57 | 63 | 41 | 47 | 30 | 27 | 31 | 97 | 74 | 40 | 46 | 65 | 62 |
Nuclear Periphery | 3 | 0 | 4 | 0 | 1 | 0 | 1 | 4 | 0 | 3 | 6 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 7 | 1 | 21 | 5 | 3 | 11 |
Nucleolus | 65 | 54 | 147 | 182 | 178 | 197 | 295 | 271 | 321 | 322 | 374 | 344 | 326 | 325 | 319 | 188 | 129 | 119 | 92 | 52 | 174 | 153 | 199 | 269 |
Peroxisomes | 0 | 0 | 0 | 3 | 1 | 3 | 3 | 3 | 4 | 0 | 4 | 2 | 3 | 2 | 2 | 4 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 1 | 7 | 4 | 11 | 12 | 25 | 33 | 16 | 20 | 34 | 4 | 17 | 18 | 1 | 0 | 2 | 0 | 0 | 3 | 1 | 4 | 7 |
Vac/Vac Membrane | 5 | 0 | 4 | 0 | 3 | 2 | 1 | 2 | 1 | 2 | 2 | 5 | 4 | 3 | 10 | 9 | 9 | 6 | 11 | 6 | 11 | 36 | 51 | 77 |
Unique Cell Count | 194 | 123 | 221 | 194 | 240 | 240 | 370 | 334 | 393 | 385 | 460 | 439 | 406 | 410 | 427 | 241 | 182 | 152 | 212 | 137 | 260 | 261 | 344 | 466 |
Labelled Cell Count | 231 | 153 | 250 | 249 | 305 | 315 | 485 | 456 | 542 | 522 | 632 | 588 | 451 | 445 | 463 | 281 | 213 | 177 | 212 | 137 | 260 | 261 | 344 | 466 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.8 | 22.7 | 21.5 | 21.4 | 18.7 | 16.2 | 15.3 | 14.2 | 14.0 | 13.7 | 12.8 | 13.3 | 26.9 | 25.5 | 23.9 | 41.6 | 37.4 | 44.3 | 25.8 | 28.7 | 28.4 |
Std Deviation (1e-4) | 4.9 | 6.2 | 6.7 | 9.3 | 6.5 | 6.6 | 5.8 | 4.9 | 5.7 | 5.4 | 4.1 | 5.2 | 12.4 | 13.8 | 12.7 | 19.4 | 17.6 | 16.2 | 9.7 | 10.0 | 9.0 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.21 | -0.41 | -0.49 | -0.6 | -0.62 | -0.65 | -0.75 | -0.7 | 0.32 | 0.25 | 0.15 | 0.95 | 0.8 | 1.04 | 0.26 | 0.42 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 2.9 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.1 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 3.9 | 4.4 | 2.4 | 3.0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.6 | 4.9 | 5.3 | 6.3 | 5.9 | 6.4 | 6.7 | 7.2 | 6.0 | -1.2 | -4.0 | -3.2 | 2.3 | 1.8 | 0.5 |
Nucleus | -7.2 | -3.3 | -5.6 | -6.3 | -6.1 | -7.1 | -7.2 | -6.9 | -6.2 | -5.2 | -7.3 | -7.0 | -5.4 | -4.3 | -2.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 6.8 | 1.8 | 3.8 | 3.6 | 3.9 | 4.2 | 4.8 | 4.3 | 3.3 | 3.8 | 3.5 | 2.2 | 2.8 | 0.9 | 2.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2.8 | 0 | 3.8 | 4.1 | 2.7 | 2.8 | 3.9 | 0 | 2.7 | 2.7 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 1.8 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.093 | 23.3629 | 23.3353 | 22.2336 | 19.8082 | 21.9438 | 20.6015 | 26.532 | 23.5402 | 22.7472 | 21.0512 | 23.0394 | 25.3298 | 26.1324 | 23.6873 | 20.7669 | 23.7979 | 22.4826 |
Actin | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0112 | 0.0002 | 0.0088 | 0.0232 | 0.0521 | 0.0015 | 0.015 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 |
Bud | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0.0003 | 0.0005 | 0.0008 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 |
Bud Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0004 | 0.0009 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Bud Site | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0043 | 0.0002 | 0.0039 | 0.0008 | 0.0047 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0003 | 0.0003 | 0.0003 | 0.0007 | 0.0005 | 0.0005 | 0.0012 | 0.0006 | 0.0004 | 0.0004 | 0.0002 | 0.0015 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0011 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0.0105 | 0 | 0.0004 | 0.0015 | 0.0005 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0416 | 0.0002 | 0.0038 | 0.0139 | 0.0051 | 0.0041 | 0.0085 | 0.0005 | 0.0001 | 0 | 0 | 0.0001 |
Eisosomes | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0.0024 | 0 | 0.0008 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0162 | 0.0002 | 0.005 | 0.0084 | 0.005 | 0.01 | 0.0033 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Golgi | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0039 | 0.0002 | 0.0058 | 0.0216 | 0.0132 | 0.0127 | 0.0014 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0076 | 0.0006 | 0.0014 | 0.003 | 0.011 | 0.0081 | 0.0391 | 0.0013 | 0.0037 | 0.0033 | 0.0016 | 0.0064 | 0.0094 | 0.0114 | 0.0047 | 0.0001 | 0.0006 | 0.0056 |
Mitochondria | 0.0028 | 0.0018 | 0.0008 | 0.0014 | 0.0068 | 0.002 | 0.0184 | 0.0063 | 0.0037 | 0.0056 | 0.0051 | 0.0119 | 0.0096 | 0.0011 | 0.0008 | 0.0015 | 0.0029 | 0.0077 |
None | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0053 | 0 | 0.0004 | 0.0021 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0266 | 0.0453 | 0.0329 | 0.062 | 0.0898 | 0.0496 | 0.0251 | 0.0041 | 0.0104 | 0.0035 | 0.0235 | 0.0411 | 0.0236 | 0.0144 | 0.0372 | 0.0064 | 0.028 | 0.0574 |
Nucleolus | 0.8252 | 0.7224 | 0.8583 | 0.7979 | 0.692 | 0.7812 | 0.6088 | 0.6967 | 0.8093 | 0.8037 | 0.6482 | 0.6972 | 0.785 | 0.738 | 0.8137 | 0.767 | 0.8188 | 0.7672 |
Nucleus | 0.121 | 0.2199 | 0.0983 | 0.1045 | 0.1569 | 0.1297 | 0.1449 | 0.2773 | 0.1332 | 0.1035 | 0.2298 | 0.179 | 0.1244 | 0.226 | 0.1313 | 0.2229 | 0.1408 | 0.1387 |
Peroxisomes | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0196 | 0.0008 | 0.003 | 0.0021 | 0.0031 | 0.0007 | 0.0027 | 0 | 0 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0011 | 0.0007 | 0.0006 | 0.0011 | 0.0032 | 0.0013 | 0.0193 | 0.0003 | 0.0006 | 0.0016 | 0.0005 | 0.0004 | 0.0033 | 0.0013 | 0.0015 | 0.0005 | 0.0035 | 0.0009 |
Vacuole | 0.0011 | 0.0008 | 0.0006 | 0.0014 | 0.0013 | 0.0024 | 0.0049 | 0.0017 | 0.0013 | 0.0006 | 0.0005 | 0.0048 | 0.0012 | 0.0006 | 0.0007 | 0.0001 | 0.0009 | 0.0018 |
Vacuole Periphery | 0.0123 | 0.0076 | 0.0059 | 0.0273 | 0.0375 | 0.0241 | 0.0222 | 0.0097 | 0.0045 | 0.0028 | 0.0043 | 0.027 | 0.009 | 0.0056 | 0.0091 | 0.0009 | 0.0034 | 0.0185 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 202.2015 | 217.0666 | 250.4919 | 207.7664 | 244.2226 | 240.9015 | 172.0707 | 186.5257 | 202.8317 | 149.5988 |
Translational Efficiency | 2.3606 | 2.3036 | 1.7787 | 2.7574 | 1.7865 | 1.5028 | 1.8604 | 1.7218 | 1.4845 | 1.91 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
130 | 185 | 149 | 1385 | 1899 | 2245 | 239 | 1298 | 2029 | 2430 | 388 | 2683 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1971.15 | 2142.19 | 2883.53 | 3324.20 | 1598.23 | 2011.55 | 3051.47 | 3329.82 | 1622.12 | 2021.50 | 2986.98 | 3326.92 |
Standard Deviation | 477.31 | 477.95 | 650.41 | 1284.17 | 360.66 | 441.69 | 612.56 | 1088.11 | 380.37 | 445.90 | 632.66 | 1193.35 |
Intensity Change Log 2 | — | 0.120049 | 0.548798 | 0.753970 | — | 0.331833 | 0.933029 | 1.058969 | — | 0.218737 | 0.733575 | 0.898553 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000989 | 0.000884 | 0.000782 | 0.002213 | 0.000707 | 0.001156 | 0.000904 | 0.001383 | 0.000725 | 0.001135 | 0.000857 | 0.001812 |
Bud Neck | 0.000978 | 0.001307 | 0.001892 | 0.004075 | 0.001164 | 0.001454 | 0.001663 | 0.008729 | 0.001152 | 0.001443 | 0.001751 | 0.006326 |
Bud Site | 0.000081 | 0.000163 | 0.000248 | 0.000838 | 0.000139 | 0.000151 | 0.000229 | 0.003357 | 0.000135 | 0.000152 | 0.000236 | 0.002057 |
Cell Periphery | 0.000014 | 0.000044 | 0.000011 | 0.000211 | 0.000024 | 0.000052 | 0.000013 | 0.000514 | 0.000023 | 0.000052 | 0.000012 | 0.000357 |
Cytoplasm | 0.000017 | 0.001553 | 0.000009 | 0.000956 | 0.000100 | 0.000041 | 0.000022 | 0.000922 | 0.000095 | 0.000156 | 0.000017 | 0.000939 |
Cytoplasmic Foci | 0.000255 | 0.002806 | 0.001176 | 0.006700 | 0.000851 | 0.000356 | 0.001826 | 0.012075 | 0.000813 | 0.000543 | 0.001576 | 0.009300 |
Eisosomes | 0.000101 | 0.000104 | 0.000124 | 0.000168 | 0.000187 | 0.000219 | 0.000156 | 0.000192 | 0.000181 | 0.000210 | 0.000144 | 0.000180 |
Endoplasmic Reticulum | 0.000339 | 0.000399 | 0.000074 | 0.000219 | 0.000210 | 0.000414 | 0.000068 | 0.000405 | 0.000218 | 0.000413 | 0.000070 | 0.000309 |
Endosome | 0.000008 | 0.000320 | 0.000010 | 0.000138 | 0.000017 | 0.000100 | 0.000006 | 0.001616 | 0.000016 | 0.000117 | 0.000008 | 0.000853 |
Golgi | 0.000043 | 0.003292 | 0.000127 | 0.000348 | 0.000057 | 0.000052 | 0.000098 | 0.001883 | 0.000056 | 0.000299 | 0.000109 | 0.001091 |
Lipid Particles | 0.000008 | 0.000054 | 0.000013 | 0.000242 | 0.000010 | 0.000267 | 0.000009 | 0.000147 | 0.000010 | 0.000251 | 0.000010 | 0.000196 |
Mitochondria | 0.001776 | 0.001531 | 0.002630 | 0.009213 | 0.001801 | 0.001556 | 0.003162 | 0.006298 | 0.001799 | 0.001554 | 0.002958 | 0.007803 |
Mitotic Spindle | 0.000721 | 0.003864 | 0.002812 | 0.002364 | 0.001727 | 0.000358 | 0.000770 | 0.005867 | 0.001662 | 0.000625 | 0.001554 | 0.004059 |
None | 0.000413 | 0.000423 | 0.000337 | 0.003622 | 0.000728 | 0.000522 | 0.000426 | 0.001279 | 0.000707 | 0.000515 | 0.000392 | 0.002488 |
Nuclear Periphery | 0.002132 | 0.006728 | 0.001129 | 0.017970 | 0.001092 | 0.001381 | 0.002661 | 0.004747 | 0.001159 | 0.001788 | 0.002072 | 0.011573 |
Nuclear Periphery Foci | 0.000364 | 0.002927 | 0.000305 | 0.004146 | 0.000649 | 0.000578 | 0.000580 | 0.001130 | 0.000631 | 0.000756 | 0.000474 | 0.002687 |
Nucleolus | 0.592869* | 0.511966* | 0.770148* | 0.564473* | 0.619657* | 0.477109* | 0.759924* | 0.552842* | 0.617941* | 0.479763* | 0.763850* | 0.558846* |
Nucleus | 0.383121* | 0.447829* | 0.168431* | 0.306771* | 0.348533* | 0.503201* | 0.172594* | 0.356840* | 0.350749* | 0.498985* | 0.170995* | 0.330994* |
Peroxisomes | 0.013032 | 0.009052 | 0.048009 | 0.021236 | 0.019372 | 0.005306 | 0.052308 | 0.023179 | 0.018965 | 0.005591 | 0.050657 | 0.022176 |
Vacuole | 0.000477 | 0.000995 | 0.000348 | 0.005097 | 0.000634 | 0.000969 | 0.000375 | 0.004663 | 0.000624 | 0.000971 | 0.000365 | 0.004887 |
Vacuole Periphery | 0.002264 | 0.003759 | 0.001385 | 0.049001 | 0.002342 | 0.004760 | 0.002208 | 0.011931 | 0.002337 | 0.004684 | 0.001892 | 0.031067 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.90 | 1.91 | -11.63 | -12.21 | -14.34 | -10.52 | -2.47 | -12.42 | -3.78 | -5.31 | -10.14 | -2.25 | -22.19 | -12.30 | -13.67 |
Bud Neck | -1.56 | -3.48 | -10.12 | -8.18 | -5.68 | -2.71 | -2.94 | -12.02 | -11.56 | -11.08 | -2.90 | -4.13 | -14.84 | -13.90 | -12.58 |
Bud Site | -1.30 | -3.12 | -3.98 | -3.34 | -2.99 | -0.47 | -2.58 | -4.89 | -4.86 | -4.76 | -0.64 | -3.40 | -5.76 | -5.67 | -5.45 |
Cell Periphery | -0.94 | 0.43 | -4.55 | -2.84 | -4.60 | -2.01 | 2.52 | -3.51 | -3.28 | -3.59 | -2.15 | 2.62 | -4.71 | -4.22 | -4.87 |
Cytoplasm | -1.00 | 0.95 | -3.90 | 0.43 | -3.93 | 0.67 | 1.07 | -3.56 | -3.87 | -3.99 | -0.57 | 1.13 | -4.83 | -3.78 | -5.59 |
Cytoplasmic Foci | -1.25 | -2.24 | -5.81 | -1.37 | -4.61 | 1.75 | -1.42 | -6.55 | -6.85 | -5.78 | 0.76 | -1.64 | -8.27 | -8.47 | -7.19 |
Eisosomes | -0.15 | -1.61 | -3.89 | -3.57 | -2.68 | -2.20 | 1.21 | -0.32 | 1.04 | -1.20 | -2.12 | 2.12 | 0.02 | 1.83 | -1.90 |
Endoplasmic Reticulum | -0.29 | 4.54 | 2.00 | 0.87 | -7.35 | -5.65 | 9.86 | -2.32 | 0.04 | -4.00 | -5.26 | 10.68 | -2.13 | 1.84 | -5.67 |
Endosome | -1.12 | -0.62 | -2.28 | 0.60 | -2.25 | -2.05 | 4.61 | -3.42 | -3.22 | -3.45 | -2.34 | 3.46 | -3.69 | -3.16 | -3.73 |
Golgi | -1.01 | -2.25 | -4.55 | 0.91 | -2.79 | 0.32 | -3.77 | -2.68 | -2.68 | -2.62 | -0.99 | -3.34 | -3.15 | -1.91 | -2.97 |
Lipid Particles | -1.53 | -0.96 | -2.08 | -1.60 | -2.02 | -1.55 | 0.47 | -3.45 | 0.65 | -3.47 | -1.57 | -0.42 | -3.10 | 0.31 | -3.08 |
Mitochondria | 1.00 | -1.31 | -14.94 | -16.01 | -8.51 | 1.55 | -3.98 | -9.69 | -10.51 | -5.91 | 1.65 | -3.48 | -17.35 | -19.03 | -10.98 |
Mitotic Spindle | -0.87 | -0.84 | -1.57 | 0.42 | 0.36 | 2.01 | 1.42 | -3.13 | -4.64 | -4.29 | 1.55 | -0.06 | -2.65 | -4.65 | -1.71 |
None | -0.19 | 1.12 | -5.24 | -5.22 | -5.38 | 1.42 | 2.43 | -3.33 | -4.99 | -6.29 | 1.43 | 2.77 | -5.30 | -6.04 | -6.58 |
Nuclear Periphery | -1.72 | 1.06 | -7.99 | -3.59 | -9.10 | -0.89 | -1.90 | -4.18 | -3.81 | -1.74 | -1.72 | -1.62 | -9.97 | -9.18 | -8.28 |
Nuclear Periphery Foci | -1.35 | 0.34 | -8.37 | -0.62 | -8.37 | 0.18 | 0.28 | -1.86 | -1.68 | -2.51 | -0.38 | 0.71 | -6.65 | -5.01 | -8.36 |
Nucleolus | 2.19 | -5.37 | 1.01 | -2.00 | 11.20 | 14.81* | -8.85 | 6.67 | -5.70 | 12.57 | 14.84* | -11.53 | 7.16 | -8.00 | 16.68 |
Nucleus | -1.69 | 6.53 | 2.56 | 5.27 | -7.71 | -15.93 | 11.22 | -1.16 | 12.23 | -11.17 | -15.75 | 14.42 | 1.96 | 17.94 | -13.17 |
Peroxisomes | 1.14 | -5.91 | -2.53 | -4.75 | 4.93 | 17.27* | -6.25 | -2.49 | -11.05 | 5.20 | 16.77 | -8.26 | -2.69 | -14.30 | 7.13 |
Vacuole | -1.05 | 1.51 | -13.04 | -6.72 | -13.42 | -2.29 | 2.37 | -4.86 | -4.44 | -5.19 | -2.46 | 2.61 | -9.47 | -8.59 | -10.19 |
Vacuole Periphery | -1.60 | 2.15 | -15.68 | -14.71 | -16.10 | -4.34 | 0.33 | -6.95 | -4.93 | -7.00 | -4.50 | 1.33 | -16.70 | -15.00 | -17.05 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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