Standard name
Human Ortholog
Description Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.29 0.44 0.54 0.62 0.56 0.58 0.49 0.45 0.43 0.39 0.39 0.39 0.55 0.36 0.45 0.16 0.05 0.07 0.07 0.09 0.12 0.17 0.15 0.16
Bud 0 0.06 0.11 0.13 0.12 0.11 0.1 0.06 0.09 0.08 0.08 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.55 0.66 0.66 0.51 0.63 0.41 0.43 0.31 0.34 0.34 0.28 0.34 0.72 0.77 0.79 0.71 0.74 0.93 0.81 0.72 0.72 0.69 0.69 0.66
Cytoplasm 0 0.08 0.05 0 0 0 0.05 0.06 0 0 0.08 0.05 0.06 0 0 0.18 0.17 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.12 0.09 0.1 0.13 0.09 0.2 0.24 0.28 0.22 0.2 0.16 0.19 0.07 0.06 0 0 0 0.07 0.05 0 0.06 0 0.06 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0 0 0 0 0 0
Mitochondria 0.36 0.3 0.44 0.73 0.58 0.6 0.51 0.52 0.69 0.75 0.73 0.76 0.45 0.42 0.71 0.18 0.05 0.13 0 0 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 55 66 80 85 144 109 130 98 76 108 25 54 66 76 107 6 3 2 17 16 21 21 27 25
Bud 8 9 17 18 31 20 27 14 16 22 5 3 2 7 13 2 4 0 1 0 0 1 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 4 2 3 5 1
Bud Site 0 1 0 2 2 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 105 99 99 69 161 76 113 67 59 93 18 47 86 163 190 27 48 28 205 131 124 88 130 102
Cytoplasm 8 12 7 4 9 7 13 13 3 9 5 7 7 9 9 7 11 0 0 5 0 0 1 2
Endoplasmic Reticulum 22 13 15 18 24 38 64 60 38 54 10 27 9 12 3 1 1 2 13 7 10 2 11 11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Golgi 24 0 1 2 6 1 2 6 4 3 0 2 0 1 0 0 4 3 0 0 0 0 0 0
Mitochondria 69 45 65 99 147 113 134 112 121 205 47 106 54 89 169 7 3 4 6 6 5 7 5 4
Nucleus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 1 1 1 7 2 2 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2 0 0 0 0 0 0 0 2 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 9 2 4 5 1 6 1 0 2 2 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 1 0 0 0 0 1 1 0 0 1 1 0 1 0 2 0 0 4 1 0 1 1
Unique Cell Count 190 150 149 136 255 187 265 217 175 274 64 140 120 213 239 38 65 30 254 182 172 129 189 155
Labelled Cell Count 297 245 286 298 533 366 488 377 322 510 114 249 228 360 494 50 76 39 254 182 172 129 189 155


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.0 16.9 12.6 12.7 13.8 12.7 12.9 12.3 11.7 12.0 11.0 10.4 17.7 17.6 15.7 21.9 23.5 25.5 18.0 18.2 18.2
Std Deviation (1e-4) 4.7 4.3 2.7 2.8 3.2 2.7 2.8 2.6 2.4 2.5 2.3 2.6 4.3 4.1 4.2 4.9 6.8 6.2 4.6 4.3 5.1
Intensity Change (Log2) – – – 0.01 0.13 0.01 0.03 -0.03 -0.11 -0.07 -0.2 -0.28 0.49 0.48 0.32 0.79 0.9 1.02 0.51 0.53 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.5 0.5 0.8 -0.9 -1.6 -1.8 -2.8 -2.0 -2.6 0.2 -3.4 -1.7 -4.2 -6.8 -4.7
Bud 0.5 0.2 -0.2 -0.4 -1.7 -0.7 -1.1 -0.8 -3.1 -3.1 -3.1 -2.1 0 -1.2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.7 -0.7 -4.7 -4.7 -6.7 -5.9 -6.4 -5.2 -5.6 0.9 2.1 2.9 0.5 1.1 3.0
Cytoplasm -0.8 -0.6 -0.4 0.1 0.5 0 -0.7 0 0.1 0.4 -0.2 -0.4 0 3.0 0
Endoplasmic Reticulum 0.8 -0.2 2.6 3.5 4.1 2.8 2.6 1.2 2.2 -0.7 -1.6 -4.0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.0 2.7 3.1 1.4 1.5 4.6 6.4 4.0 5.5 0.2 -0.3 5.3 -2.8 -5.6 -3.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.5361 20.4832 20.3028 17.6945 22.0635 21.3965 20.2028 19.6106 19.9041 20.9425 18.5249 19.9287 20.0652 17.1776 17.6602 13.1507 18.6931 17.9903
Actin 0.1204 0.0162 0.0362 0.0533 0.0415 0.0307 0.0961 0.0073 0.0238 0.0299 0.0358 0.01 0.0875 0.0061 0.0614 0.0209 0.026 0.0043
Bud 0.0026 0.0019 0.006 0.002 0.0011 0.0007 0.002 0.0015 0.0025 0.0011 0.0013 0.0007 0.0053 0.0012 0.003 0.0022 0.0008 0.0007
Bud Neck 0.0106 0.0231 0.0216 0.0112 0.0341 0.0177 0.0108 0.0098 0.015 0.0124 0.0047 0.0289 0.0067 0.0107 0.0121 0.0055 0.005 0.0205
Bud Periphery 0.0181 0.0061 0.0475 0.0335 0.0171 0.0104 0.0101 0.0071 0.0272 0.0273 0.0104 0.0137 0.0179 0.0062 0.0327 0.0355 0.0157 0.0116
Bud Site 0.0275 0.3416 0.0635 0.0045 0.0026 0.0012 0.0277 0.284 0.0409 0.0024 0.0017 0.0037 0.0286 0.2009 0.0373 0.0035 0.0008 0.0021
Cell Periphery 0.4472 0.4668 0.6243 0.6438 0.5261 0.6728 0.4718 0.5336 0.7198 0.7746 0.5991 0.6513 0.4846 0.5489 0.6667 0.588 0.6379 0.668
Cytoplasm 0.009 0.0048 0.0034 0.0048 0.0007 0.0011 0.0084 0.0056 0.0031 0.0018 0.0029 0.002 0.0032 0.0048 0.0039 0.0044 0.0017 0.0022
Cytoplasmic Foci 0.0117 0.0003 0.003 0.0166 0.0015 0.0011 0.0385 0.0012 0.0037 0.0033 0.018 0.0012 0.0246 0.0001 0.0016 0.0158 0.0033 0.0001
Eisosomes 0.181 0.116 0.1102 0.0554 0.2768 0.1939 0.1979 0.1047 0.0927 0.0983 0.1898 0.2473 0.2557 0.1845 0.1296 0.0764 0.2677 0.2621
Endoplasmic Reticulum 0.013 0.0028 0.0107 0.0209 0.0043 0.011 0.0181 0.0064 0.0148 0.0235 0.014 0.0121 0.0095 0.015 0.0146 0.0175 0.0134 0.0143
Endosome 0.0233 0.0001 0.0017 0.0244 0.023 0.0017 0.0119 0.0017 0.0023 0.0008 0.0396 0.001 0.0027 0.0001 0.0013 0.0073 0.0051 0.0001
Golgi 0.0111 0.0002 0.0025 0.0225 0.0283 0.0034 0.0075 0.0006 0.0043 0.0028 0.0264 0.0025 0.0047 0.0003 0.0047 0.0031 0.0059 0.0009
Lipid Particles 0.0192 0.0004 0.0026 0.0199 0.0079 0.0026 0.0139 0.001 0.0016 0.0021 0.0121 0.002 0.0043 0.0003 0.0045 0.0161 0.0029 0.0005
Mitochondria 0.0358 0.0085 0.038 0.0545 0.0224 0.0388 0.0181 0.0132 0.0241 0.0018 0.0218 0.0138 0.0432 0.0027 0.0092 0.1634 0.0041 0.0032
None 0.0069 0.0006 0.0025 0.0085 0.0007 0.0007 0.0173 0.0007 0.0006 0.0005 0.001 0.0007 0.0011 0.0006 0.0007 0.017 0.0016 0.0009
Nuclear Periphery 0.0173 0.002 0.0033 0.0038 0.0011 0.0022 0.0085 0.0094 0.0035 0.0039 0.0049 0.0022 0.0037 0.0058 0.0031 0.0038 0.0023 0.0022
Nucleolus 0.0023 0.0008 0.0012 0.0011 0.0005 0.0004 0.002 0.0008 0.0006 0.0004 0.0003 0.0003 0.0009 0.0005 0.0003 0.0012 0.0001 0.0007
Nucleus 0.0099 0.0007 0.0011 0.0009 0.0004 0.0004 0.003 0.0016 0.0007 0.0004 0.0007 0.0004 0.0019 0.0005 0.0004 0.0011 0.0002 0.0008
Peroxisomes 0.0112 0.0005 0.0033 0.0038 0.0005 0.0004 0.0061 0.0004 0.0046 0.002 0.0038 0.0004 0.0016 0.0002 0.0036 0.0048 0.0008 0.0001
Punctate Nuclear 0.0072 0 0.0004 0.0039 0.0001 0 0.0147 0.0003 0 0.0001 0.0049 0.0004 0.0047 0 0.0001 0.0035 0.0003 0
Vacuole 0.0115 0.0062 0.0153 0.0084 0.0068 0.0073 0.0138 0.0079 0.0129 0.01 0.0058 0.0048 0.0067 0.0101 0.0086 0.0074 0.0039 0.0041
Vacuole Periphery 0.0034 0.0003 0.0017 0.0025 0.0028 0.0013 0.0018 0.0011 0.0013 0.0008 0.0011 0.0005 0.001 0.0006 0.0006 0.0016 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.1399 94.852 99.7374 108.8882 70.1734 85.5985 113.5375 115.0178 105.6448 87.0198
Translational Efficiency 0.9371 0.8914 1.0439 0.7994 0.851 0.8291 0.7365 0.7228 0.7831 0.6789

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
86 1110 349 1373 1751 1954 316 157 1837 3064 665 1530

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1322.78 1557.01 2769.73 1628.52 1433.59 1553.14 2018.32 1766.29 1428.40 1554.54 2412.67 1642.66
Standard Deviation 297.15 298.16 465.26 356.06 285.38 309.24 359.63 287.84 286.89 305.28 562.02 352.16
Intensity Change Log 2 — 0.235205 1.066172 0.299988 — 0.115555 0.493522 0.301090 — 0.174215 0.796669 0.300561

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.027380 0.011382 0.000953 0.014689 0.008296 0.011261 0.004391 0.010317 0.009189 0.011305 0.002587 0.014241
Bud Neck 0.014026 0.007551 0.003968 0.006448 0.004543 0.004834 0.005624 0.003495 0.004987 0.005818 0.004755 0.006145
Bud Site 0.046631 0.007367 0.001858 0.031082 0.004686 0.009778 0.006069 0.004531 0.006649 0.008904 0.003859 0.028358
Cell Periphery 0.654935* 0.779743* 0.866860* 0.674733* 0.817641* 0.866104* 0.876505* 0.930854* 0.810024* 0.834818* 0.871443* 0.701015*
Cytoplasm 0.001593 0.000206 0.000043 0.023709 0.000139 0.000325 0.000069 0.000277 0.000207 0.000282 0.000055 0.021304
Cytoplasmic Foci 0.003566 0.000888 0.000084 0.038322 0.000807 0.000735 0.000073 0.000697 0.000936 0.000790 0.000079 0.034461
Eisosomes 0.008487 0.083987 0.051664 0.013555 0.078220 0.052773 0.069104 0.028439 0.074956 0.064081 0.059951 0.015083
Endoplasmic Reticulum 0.202512* 0.081863 0.022232 0.052911 0.063071 0.038295 0.024076 0.008818 0.069599 0.054078 0.023109 0.048386
Endosome 0.016292 0.006903 0.001810 0.028608 0.005756 0.004217 0.001641 0.002058 0.006249 0.005190 0.001730 0.025883
Golgi 0.009420 0.005200 0.003715 0.034301 0.002734 0.001797 0.001753 0.002793 0.003047 0.003030 0.002782 0.031068
Lipid Particles 0.002644 0.002078 0.003584 0.053414 0.001151 0.001490 0.001631 0.000063 0.001221 0.001703 0.002656 0.047939
Mitochondria 0.003684 0.002793 0.000377 0.004168 0.003536 0.002045 0.000517 0.002192 0.003542 0.002316 0.000444 0.003965
Mitotic Spindle 0.000929 0.002316 0.002167 0.001370 0.001296 0.001093 0.001115 0.000700 0.001278 0.001536 0.001667 0.001301
None 0.000080 0.000112 0.000049 0.001388 0.000295 0.000088 0.000042 0.000035 0.000285 0.000097 0.000046 0.001249
Nuclear Periphery 0.000732 0.002497 0.007773 0.001207 0.002735 0.002063 0.001777 0.000691 0.002642 0.002220 0.004924 0.001154
Nuclear Periphery Foci 0.000553 0.000698 0.000255 0.000609 0.000229 0.000130 0.000147 0.000087 0.000244 0.000335 0.000204 0.000556
Nucleolus 0.000036 0.000063 0.000044 0.000146 0.000064 0.000040 0.000027 0.000025 0.000062 0.000048 0.000036 0.000134
Nucleus 0.000223 0.000220 0.000209 0.001063 0.000178 0.000128 0.000137 0.000083 0.000180 0.000161 0.000175 0.000962
Peroxisomes 0.001668 0.000590 0.000034 0.005357 0.002463 0.000871 0.000024 0.000065 0.002425 0.000769 0.000029 0.004814
Vacuole 0.002662 0.002259 0.030160 0.009595 0.001040 0.001334 0.004594 0.002589 0.001116 0.001669 0.018012 0.008876
Vacuole Periphery 0.001949 0.001284 0.002160 0.003325 0.001122 0.000600 0.000684 0.001191 0.001160 0.000848 0.001459 0.003106

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.73 2.92 1.38 -1.41 -8.64 -1.62 2.11 -0.30 0.14 -0.87 -1.29 4.71 -2.55 -1.55 -6.81
Bud Neck 1.74 2.73 2.04 1.11 -2.88 -0.56 -0.98 0.66 0.83 1.13 -1.62 0.34 -1.60 -0.47 -1.65
Bud Site 2.88 3.29 1.11 -7.67 -10.19 -3.53 -0.98 0.09 2.59 0.77 -1.75 2.56 -8.04 -7.13 -9.28
Cell Periphery -3.50 -5.92 -0.52 7.87 13.81 -6.49 -4.91 -8.38 -4.93 -3.37 -3.47 -6.93 9.40 12.23 14.05
Cytoplasm 3.35 3.78 -9.62 -10.38 -10.46 -3.21 2.99 -1.02 0.33 -1.54 -1.59 5.59 -10.35 -10.31 -10.42
Cytoplasmic Foci 1.63 2.15 -10.80 -13.39 -13.73 0.32 6.82 0.18 0.06 -1.01 0.72 6.69 -13.30 -13.35 -13.66
Eisosomes -15.43 -6.63 -2.56 14.85 5.96 5.79 1.04 7.33 3.86 4.13 2.62 2.46 16.51 18.73 8.68
Endoplasmic Reticulum 4.40 6.64 5.51 4.95 -6.73 5.80 7.29 11.89 7.65 3.04 3.59 10.56 4.50 1.48 -6.40
Endosome 2.61 4.05 -2.99 -10.23 -12.88 3.94 12.67 3.01 1.76 -0.34 2.68 13.61 -10.33 -10.94 -12.84
Golgi 1.23 1.67 -5.88 -11.24 -11.88 2.41 2.29 -0.03 -0.51 -0.53 0.04 0.68 -12.11 -12.10 -12.20
Lipid Particles 0.61 -0.44 -13.59 -13.75 -11.86 -0.72 -0.42 4.18 3.64 1.41 -1.16 -1.18 -14.00 -13.82 -12.82
Mitochondria 0.85 3.44 -0.41 -1.64 -5.11 3.65 9.00 0.76 -0.08 -0.96 3.20 9.62 -0.57 -2.30 -5.12
Mitotic Spindle -1.88 -6.53 -1.74 1.19 2.85 3.19 2.05 5.03 3.38 3.16 -0.97 -4.39 -0.28 0.59 1.46
None -2.55 2.67 -2.03 -1.98 -2.08 2.19 2.69 2.76 5.96 1.38 2.09 2.66 -1.65 -1.99 -2.08
Nuclear Periphery -9.30 -10.86 -3.27 8.17 10.26 4.19 4.77 12.16 9.34 5.71 2.87 -5.99 10.79 10.34 10.29
Nuclear Periphery Foci -0.51 3.20 -0.53 0.36 -5.44 1.21 0.98 1.72 2.84 2.52 -0.69 0.51 -3.26 -1.32 -6.11
Nucleolus -1.66 -0.91 -2.31 -1.66 -2.18 8.28 13.48 11.03 4.99 0.71 2.16 9.72 -1.70 -1.99 -2.32
Nucleus -0.12 0.49 -2.66 -2.56 -2.71 5.76 3.22 5.87 2.93 3.00 0.49 0.49 -2.76 -2.80 -2.78
Peroxisomes 1.22 1.88 -3.62 -8.63 -9.91 2.92 4.70 4.61 4.77 -1.13 3.25 4.83 -3.47 -8.14 -9.89
Vacuole 0.61 -12.75 -5.64 -6.21 8.55 -2.55 -5.34 -2.61 -2.10 2.26 -3.77 -13.53 -7.60 -7.01 5.58
Vacuole Periphery 2.10 -0.62 -2.45 -4.12 -2.30 3.47 2.78 -0.14 -1.21 -1.03 2.11 -1.76 -4.26 -5.16 -3.70
Standard name
Human Ortholog
Description Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localized to the bud via specific mRNA transport; non-tagged protein detected in a phosphorylated state in mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
Localization
Cell Percentages cell periphery (62%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-2

Tcb3

Tcb3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tcb3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available