Standard name
Human Ortholog
Description Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.12 0.17 0.16 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.05 0.12 0.14 0.11 0.11 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.92 0.94 0.89 0.87 0.79 0.79 0.68 0.65 0.62 0.62 0.56 0.56 0.86 0.8 0.76 0.75 0.71 0.71 0.85 0.88 0.81 0.7 0.71 0.79
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.28 0.26 0.31 0.24 0.35 0.35 0.49 0.55 0.54 0.54 0.65 0.65 0.16 0.16 0.14 0.06 0.1 0.12 0.12 0.08 0.13 0.2 0.18 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0.08 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 3 0 4 0 0 6 1 0 0 0 0 0 2 3 4 7 25
Bud Neck 0 1 0 0 1 1 0 0 3 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 2 2 19 16 17 10 6 8 6 14 16 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
Cytoplasm 6 3 4 5 5 5 10 10 11 5 7 5 72 92 102 49 50 30 0 0 0 0 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 8 1 0 0 1 0 1 1
Endosome 1 0 0 1 1 1 0 2 0 0 0 0 3 3 5 3 0 1 1 0 2 2 5 3
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0 2 2 0 1
Mitochondria 3 5 4 7 11 11 45 40 58 67 29 33 1 1 1 2 4 10 1 0 2 3 7 0
Nucleus 242 504 411 263 301 301 363 479 301 302 142 165 776 733 674 220 218 148 225 491 409 479 653 735
Nuclear Periphery 0 0 1 0 0 0 9 3 5 13 0 3 0 2 1 1 0 0 0 1 0 0 1 0
Nucleolus 74 141 144 71 135 135 260 403 262 262 164 190 145 147 125 19 29 24 32 43 64 135 160 89
Peroxisomes 2 0 1 1 0 0 0 0 0 0 0 0 3 11 12 1 1 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 1 1 0 2 0 22 49 54 2 2 1 0 1 1 2 8 8
Vac/Vac Membrane 1 4 2 0 3 3 2 7 3 5 0 0 4 2 6 9 17 11 3 15 12 53 63 58
Unique Cell Count 263 538 463 301 383 382 534 737 483 488 254 294 904 917 884 295 305 208 266 560 504 689 915 937
Labelled Cell Count 329 658 568 349 459 459 708 964 661 668 350 404 1039 1058 996 309 331 227 266 560 504 689 915 937


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 21.4 24.1 15.5 14.9 15.4 15.4 12.3 12.9 12.9 11.6 11.6 12.0 25.1 25.8 25.3 25.7 29.9 27.1 16.5 17.3 17.8
Std Deviation (1e-4) 8.7 6.8 4.4 4.3 4.6 4.6 4.0 4.6 4.6 4.4 3.6 3.6 10.0 12.2 13.6 16.3 16.9 16.2 4.7 4.8 5.3
Intensity Change (Log2) – – – -0.05 -0.01 -0.01 -0.34 -0.26 -0.27 -0.41 -0.42 -0.36 0.7 0.74 0.71 0.73 0.95 0.81 0.09 0.16 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 4.1 3.2 4.1 0 0 0 0 0 2.9 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 1.4 0.8 1.7 0 0 0 5.4 6.3 6.9 8.3 8.2 7.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.2 2.2 5.5 4.1 6.9 7.5 6.5 6.4 0 0 0 0 0 0
Nucleus -0.6 -4.0 -4.0 -7.9 -9.2 -9.4 -9.6 -10.0 -10.3 -1.5 -4.1 -5.5 -5.1 -6.1 -5.7
Nuclear Periphery 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0 0
Nucleolus -2.3 1.3 1.3 5.6 8.0 7.2 7.0 8.7 9.1 -6.5 -6.5 -7.4 -8.1 -7.0 -5.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 3.0 4.8 5.2 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 4.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.3694 21.5438 19.7702 19.3609 21.1461 20.3979 17.4783 21.6904 21.2355 19.9169 16.7542 19.686 19.0444 24.0203 21.856 18.6676 19.274 21.3575
Actin 0.0285 0.001 0.0067 0 0.0002 0 0.0214 0 0.0049 0 0.0176 0 0.0088 0 0.0114 0.0426 0.0315 0
Bud 0.0016 0.0004 0.0001 0 0.0001 0 0.0005 0 0.0005 0.0001 0.0009 0 0.0011 0 0.0001 0.0015 0.0006 0
Bud Neck 0.0021 0.0001 0.001 0 0.0002 0.0001 0.0002 0 0.0001 0 0.0005 0.0002 0.0005 0 0.0001 0.0013 0.0002 0.0001
Bud Periphery 0.0022 0.0003 0.0002 0 0.0002 0 0.0005 0 0.0005 0.0001 0.0011 0.0001 0.0013 0 0.0002 0.0011 0.0008 0
Bud Site 0.0038 0.004 0.0053 0 0.0006 0 0.0009 0 0.0013 0 0.0013 0 0.0036 0.0001 0.0012 0.0107 0.0011 0
Cell Periphery 0.0011 0.0001 0.0002 0 0.0004 0.0001 0.0001 0 0.0003 0.0002 0.0003 0.0001 0.0008 0.0002 0.0001 0.0004 0.0005 0.0001
Cytoplasm 0.0148 0.0007 0.004 0 0.0002 0 0.0186 0 0.0005 0 0.0034 0.0001 0.0132 0.0001 0 0.005 0.0006 0
Cytoplasmic Foci 0.0207 0.0006 0.0094 0 0.0097 0 0.0072 0 0.0038 0.001 0.0095 0.0017 0.0302 0.0047 0.0001 0.0126 0.0245 0
Eisosomes 0.0008 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0 0.0002 0 0.0001 0.0004 0.0001 0
Endoplasmic Reticulum 0.008 0.0001 0.0009 0 0.0001 0 0.0019 0 0.0001 0 0.004 0 0.0046 0 0 0.0006 0.0001 0
Endosome 0.0382 0.0028 0.0042 0 0.0072 0 0.0145 0 0.0015 0 0.0157 0.0002 0.0242 0 0.0001 0.0053 0.0019 0
Golgi 0.0096 0.0013 0.0017 0 0.0022 0 0.0093 0 0.0011 0 0.0033 0 0.0065 0 0.0002 0.0052 0.0048 0
Lipid Particles 0.0132 0 0.0074 0 0.0256 0 0.0121 0 0.0014 0.0021 0.0059 0.0008 0.0172 0.0002 0.0001 0.0032 0.0047 0
Mitochondria 0.036 0.0007 0.0141 0.0001 0.0528 0.0003 0.0057 0.0012 0.0154 0.0027 0.033 0.0002 0.0063 0.0005 0.0007 0.0104 0.0088 0.0003
None 0.0134 0 0.0028 0 0.0004 0 0.0026 0 0.0005 0.0001 0.0018 0 0.0113 0.0001 0 0.0014 0.0012 0
Nuclear Periphery 0.0074 0.0005 0.0017 0.0003 0.0013 0.0001 0.0252 0.0007 0.002 0.0004 0.0504 0.0008 0.0245 0.0005 0.001 0.0015 0.0014 0.0002
Nucleolus 0.1289 0.0277 0.0319 0.0311 0.3444 0.1782 0.1026 0.0262 0.0406 0.0935 0.3222 0.1834 0.0991 0.0411 0.046 0.0711 0.2526 0.1476
Nucleus 0.6112 0.9587 0.8997 0.9683 0.5396 0.8209 0.6938 0.9712 0.9202 0.8972 0.4945 0.7998 0.7047 0.9491 0.9381 0.8081 0.629 0.8508
Peroxisomes 0.0058 0 0.0025 0 0.0091 0 0.0033 0 0.0011 0.0001 0.0014 0 0.0073 0 0.0002 0.0137 0.0196 0
Punctate Nuclear 0.0411 0.0001 0.0026 0 0.0006 0.0001 0.0705 0 0.0011 0.0002 0.0257 0.0122 0.028 0.003 0 0.0019 0.0067 0.0007
Vacuole 0.0087 0.0007 0.0013 0 0.0031 0.0001 0.0075 0.0001 0.001 0.0006 0.0023 0.0002 0.0034 0.0002 0.0001 0.0009 0.0035 0.0001
Vacuole Periphery 0.0029 0.0002 0.0019 0 0.0019 0.0001 0.0017 0.0003 0.0021 0.0016 0.0052 0.0002 0.0031 0.0001 0.0002 0.0013 0.0057 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.8897 26.7552 37.9423 53.8081 59.0699 45.8666 45.2373 46.2546 53.0544 65.3456
Translational Efficiency 3.4886 3.7985 2.1457 1.9238 2.2266 3.6317 2.0699 1.7358 1.7895 1.7953

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1052 1345 1077 1146 158 1865 1598 1280 1210 3210 2675 2426

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1481.83 1710.09 1674.06 2496.53 1653.51 1602.92 1946.55 2263.43 1504.25 1647.82 1836.84 2373.54
Standard Deviation 261.53 410.00 466.90 751.63 338.43 414.05 434.47 700.70 278.87 415.74 467.32 734.48
Intensity Change Log 2 — 0.206692 0.175971 0.752544 — -0.044829 0.235388 0.452979 — 0.079521 0.207611 0.602328

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000706 0.000549 0.000530 0.000880 0.000500 0.000520 0.000672 0.002024 0.000679 0.000532 0.000615 0.001484
Bud Neck 0.004558 0.002843 0.003554 0.009591 0.001329 0.003683 0.005963 0.012895 0.004137 0.003331 0.004993 0.011334
Bud Site 0.003125 0.000768 0.000353 0.002444 0.000145 0.000631 0.000629 0.007708 0.002736 0.000689 0.000518 0.005221
Cell Periphery 0.000136 0.000048 0.000022 0.000421 0.000007 0.000138 0.000099 0.000685 0.000119 0.000100 0.000068 0.000560
Cytoplasm 0.002017 0.000172 0.000024 0.001101 0.000005 0.000163 0.000084 0.001236 0.001754 0.000167 0.000060 0.001172
Cytoplasmic Foci 0.004635 0.001177 0.000167 0.001875 0.000087 0.000477 0.000345 0.003016 0.004041 0.000770 0.000274 0.002477
Eisosomes 0.000211 0.000172 0.000146 0.000098 0.000118 0.000197 0.000179 0.000140 0.000199 0.000187 0.000166 0.000120
Endoplasmic Reticulum 0.000479 0.000297 0.000136 0.000363 0.000310 0.000414 0.000303 0.000539 0.000457 0.000365 0.000235 0.000456
Endosome 0.000125 0.000022 0.000003 0.000701 0.000002 0.000114 0.000290 0.002112 0.000109 0.000076 0.000175 0.001445
Golgi 0.000322 0.000034 0.000020 0.001560 0.000016 0.000096 0.000122 0.004734 0.000282 0.000070 0.000081 0.003235
Lipid Particles 0.000103 0.000025 0.000002 0.000123 0.000003 0.000087 0.000141 0.000345 0.000090 0.000061 0.000085 0.000240
Mitochondria 0.001274 0.001266 0.001210 0.003171 0.000855 0.000900 0.001377 0.041727 0.001219 0.001054 0.001310 0.023514
Mitotic Spindle 0.004511 0.000527 0.000019 0.002309 0.000002 0.001321 0.001211 0.006113 0.003922 0.000988 0.000731 0.004316
None 0.000741 0.001503 0.000555 0.002017 0.000447 0.001184 0.000567 0.001884 0.000703 0.001318 0.000562 0.001947
Nuclear Periphery 0.000035 0.000022 0.000018 0.000380 0.000006 0.000051 0.000057 0.000603 0.000032 0.000039 0.000042 0.000497
Nuclear Periphery Foci 0.000119 0.000096 0.000001 0.000204 0.000002 0.000305 0.000024 0.001105 0.000104 0.000217 0.000015 0.000679
Nucleolus 0.065141 0.043812 0.041985 0.029551 0.046641 0.033309 0.021742 0.022727 0.062725 0.037710 0.029892 0.025951
Nucleus 0.905070* 0.942579* 0.945875* 0.936853* 0.938595* 0.953576* 0.963239* 0.878685* 0.909448* 0.948968* 0.956248* 0.906163*
Peroxisomes 0.004900 0.003567 0.005094 0.003175 0.010691 0.001849 0.002343 0.002814 0.005656 0.002569 0.003450 0.002984
Vacuole 0.000837 0.000249 0.000124 0.001982 0.000141 0.000519 0.000413 0.004994 0.000746 0.000406 0.000297 0.003571
Vacuole Periphery 0.000954 0.000273 0.000162 0.001200 0.000096 0.000464 0.000201 0.003915 0.000842 0.000384 0.000186 0.002633

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.38 1.80 -1.68 -4.23 -5.85 -0.87 -1.97 -4.07 -3.98 -3.48 1.58 0.62 -3.73 -4.74 -4.23
Bud Neck 2.94 1.96 -4.56 -7.11 -6.52 -6.91 -13.03 -9.43 -7.48 -5.66 1.61 -1.41 -7.81 -10.09 -8.07
Bud Site 2.64 3.37 0.86 -2.45 -3.68 -3.56 -7.12 -5.47 -5.01 -5.09 2.72 3.11 -1.96 -5.58 -6.03
Cell Periphery 1.58 2.08 -2.59 -4.09 -4.43 -3.71 -2.05 -3.64 -2.82 -3.06 0.41 1.07 -3.64 -4.20 -4.53
Cytoplasm 2.13 2.28 1.13 -1.39 -1.66 -3.57 -4.49 -2.77 -2.48 -2.65 2.11 2.24 0.70 -2.78 -3.07
Cytoplasmic Foci 2.10 3.00 1.83 -0.66 -3.29 -1.82 -2.58 -3.95 -3.22 -3.63 2.40 2.91 1.03 -3.05 -4.71
Eisosomes 0.61 1.03 1.79 5.51 4.70 -5.00 -3.39 -1.22 2.90 1.85 0.22 0.60 1.45 5.27 3.45
Endoplasmic Reticulum 1.68 5.15 1.42 -0.63 -4.68 -2.07 0.10 -3.11 -1.38 -3.08 1.19 3.64 0.05 -1.35 -4.67
Endosome 2.61 3.13 -2.24 -2.77 -2.87 -1.90 -1.42 -3.57 -3.33 -2.89 0.73 -0.38 -3.94 -4.06 -3.57
Golgi 2.38 2.52 -1.43 -1.86 -1.88 -2.83 -1.33 -3.56 -3.49 -3.47 2.00 1.84 -3.59 -3.94 -3.93
Lipid Particles 1.42 1.94 -0.15 -1.73 -2.31 -1.72 -1.04 -4.80 -2.72 -1.39 0.50 0.18 -2.08 -3.06 -1.75
Mitochondria 0.00 0.33 -6.34 -5.11 -8.00 -0.78 -6.74 -8.10 -8.09 -7.98 0.73 -0.66 -8.30 -8.37 -8.27
Mitotic Spindle 2.36 2.75 1.17 -1.98 -2.80 -2.57 -1.91 -3.98 -2.84 -2.83 2.00 2.17 -0.07 -3.38 -3.61
None -0.96 3.05 -2.43 -0.55 -2.90 -2.00 -1.53 -3.29 -1.21 -2.96 -1.53 2.24 -3.61 -1.23 -4.14
Nuclear Periphery 2.53 3.85 -6.19 -6.45 -6.53 -5.69 -7.17 -4.86 -4.46 -4.42 -1.15 -1.63 -6.74 -6.63 -6.60
Nuclear Periphery Foci 0.20 2.65 -0.69 -0.72 -2.89 -2.18 -1.74 -1.69 -1.11 -1.66 -1.15 2.32 -1.58 -1.17 -1.94
Nucleolus 5.01 5.21 7.46 3.32 2.76 2.19 4.11 3.70 3.16 -0.56 7.13 9.32 9.68 4.36 1.25
Nucleus -6.20 -6.87 -4.14 1.53 2.19 -1.87 -3.13 5.95 9.30 10.54 -7.72 -9.16 0.52 8.72 10.24
Peroxisomes 2.32 -0.26 2.14 0.42 2.40 3.80 3.54 3.28 -2.26 -0.96 5.54 3.65 3.99 -1.31 1.02
Vacuole 4.00 5.15 -1.83 -2.94 -3.19 -3.12 -2.02 -5.10 -4.61 -4.74 2.47 3.26 -4.68 -5.43 -5.65
Vacuole Periphery 1.35 1.58 -0.43 -2.36 -2.66 -2.01 -2.82 -4.35 -3.81 -4.21 1.01 1.50 -2.61 -4.39 -4.91
Standard name
Human Ortholog
Description Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Pob3

Pob3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pob3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available