Standard name
Human Ortholog
Description Endoplasmic reticulum (ER) localized integral membrane protein; may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0.08 0.11 0.13 0 0 0 0.15 0 0
Cytoplasm 0.48 0.6 0.72 0.62 0.56 0.48 0.5 0.54 – 0.59 0.48 0.39 – – – 0.64 0.64 0.61 0.05 0.22 0.13 0 0 0
Endoplasmic Reticulum 0.14 0.32 0.11 0 0.05 0.12 0.05 0 – 0 0.09 0.11 – – – 0 0 0.05 0.82 0.6 0.73 0.51 0.25 0.57
Endosome 0.05 0 0 0 0.05 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0.11
Mitochondria 0 0 0.11 0.14 0.25 0.39 0.43 0.31 – 0.26 0.45 0.53 – – – 0.07 0 0 0 0 0 0 0 0.13
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0.24 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0.23 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.34 0.11 0.1 0.27 0.25 0.17 0.19 0.22 – 0.26 0.16 0.13 – – – 0.23 0.29 0.3 0 0 0 0.32 0.21 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 4 2 2 0 2 0 0 – 0 1 6 – – – 3 1 2 14 10 14 0 0 0
Bud 0 2 4 2 6 21 13 14 – 12 28 35 – – – 0 0 0 3 4 4 0 0 0
Bud Neck 0 0 1 0 0 0 0 0 – 0 0 0 – – – 0 0 1 3 10 4 0 0 0
Bud Site 0 0 0 0 0 2 1 1 – 2 3 4 – – – 0 0 0 – – – – – –
Cell Periphery 19 8 9 17 15 12 11 16 – 15 37 15 – – – 20 32 40 3 7 10 0 0 0
Cytoplasm 264 291 400 337 395 372 536 520 – 640 458 445 – – – 167 193 193 39 128 86 0 0 0
Endoplasmic Reticulum 77 156 60 20 32 92 52 11 – 23 85 126 – – – 10 7 16 666 357 504 1 1 4
Endosome 26 3 10 19 37 9 10 36 – 43 13 14 – – – 2 6 5 9 8 7 0 0 0
Golgi 3 0 1 0 0 0 0 0 – 0 0 0 – – – 1 2 1 3 2 1 0 0 0
Mitochondria 20 4 64 76 176 301 457 300 – 285 434 606 – – – 19 7 12 26 24 27 0 0 0
Nucleus 2 2 1 4 5 9 12 19 – 10 1 16 – – – 10 2 4 0 0 0 0 0 0
Nuclear Periphery 4 3 0 2 12 7 34 6 – 14 6 42 – – – 0 0 2 10 3 3 0 0 0
Nucleolus 1 0 0 0 1 1 5 2 – 3 0 6 – – – 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 2 0 0 0 – 1 0 0 – – – 0 0 3 0 0 0 0 0 0
SpindlePole 0 0 1 1 2 0 1 1 – 4 3 1 – – – 0 1 0 1 1 3 0 0 0
Vac/Vac Membrane 187 55 58 147 174 133 201 207 – 287 150 153 – – – 60 88 96 25 25 9 0 0 0
Unique Cell Count 550 484 558 543 703 780 1071 955 1094 963 1154 261 301 319 818 593 688 3 4 7
Labelled Cell Count 607 528 611 627 857 961 1333 1133 1339 1219 1469 292 339 375 818 593 688 3 4 7


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.7 4.9 5.3 5.2 4.9 4.3 4.7 – 4.7 4.5 4.4 – – – 7.1 7.3 7.4 – – –
Std Deviation (1e-4) 1.0 0.8 1.2 1.2 1.4 1.0 1.2 1.2 – 1.0 0.9 0.9 – – – 1.1 1.4 1.3 – – –
Intensity Change (Log2) – – – 0.11 0.09 0.01 -0.19 -0.06 – -0.07 -0.11 -0.16 – – – 0.53 0.57 0.6 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Bud 0 0 2.6 0 0 – 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Cell Periphery 1.7 0.7 -0.1 0 0 – 0 0 0 – – – 4.4 5.9 6.8
Cytoplasm -3.4 -5.7 -8.8 -8.4 -6.6 – -5.2 -9.1 -12.8 – – – -2.2 -2.3 -3.4
Endoplasmic Reticulum -4.5 -4.2 0.6 0 0 – 0 0 0 – – – -3.3 -4.4 -2.9
Endosome 1.8 3.2 -1.0 0 0 – 0 0 0 – – – 0 0.2 -0.2
Golgi 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Mitochondria 1.3 6.1 11.0 0 0 – 0 0 0 – – – -1.9 -4.6 -3.9
Nucleus 0 0 0 0 3.0 – 0 0 2.4 – – – 0 0 0
Nuclear Periphery 0 3.1 0 0 0 – 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 – – – 0 0 0
Vacuole 7.1 6.5 3.4 4.4 5.6 – 7.5 2.8 1.7 – – – 4.8 7.0 7.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.4743 4.6539 4.9193 4.7215 3.8041 4.8196 8.562 8.553 8.3951 7.3853 7.4832 8.4541 6.7832 7.9144 7.5543 8.3058 6.1744 7.2149
Actin 0.002 0.0005 0.0016 0.0115 0.0259 0.0022 0.0118 0.0001 0.0023 0.0075 0.0285 0.0053 0.0553 0.0001 0.0006 0 0.0149 0.0308
Bud 0 0 0 0.0001 0.0004 0 0.0001 0 0.0001 0 0.0015 0.0002 0.0002 0 0 0 0.0006 0.0003
Bud Neck 0.0001 0.0006 0.0009 0.0208 0.0005 0.0004 0.0008 0.0001 0.0006 0.0001 0.0003 0.0005 0.001 0.0002 0.0003 0 0.002 0.0007
Bud Periphery 0 0.0001 0.0001 0.0004 0.0008 0 0.0002 0.0001 0.0008 0.0001 0.0038 0.0006 0.0002 0 0 0 0.0013 0.0004
Bud Site 0.0001 0.0006 0.0027 0.001 0.0032 0 0.0009 0.0002 0.0001 0.0001 0.0037 0.0001 0.002 0.012 0 0 0.0009 0.0004
Cell Periphery 0.0019 0.004 0.001 0.003 0.0003 0.0003 0.0006 0.0025 0.001 0.0003 0.0008 0.0005 0.0003 0.0063 0.0013 0.0003 0.0006 0.0003
Cytoplasm 0.0239 0.0572 0.0352 0.0227 0.0649 0.0159 0.0168 0.0257 0.0129 0.0029 0.0082 0.0173 0.0253 0.0307 0.0145 0.0034 0.0265 0.007
Cytoplasmic Foci 0.0014 0.0009 0.0022 0.002 0.0042 0.0011 0.0097 0.0006 0.0019 0.0007 0.0083 0.0007 0.0279 0.0036 0.0004 0 0.004 0.0071
Eisosomes 0.0001 0 0.0001 0.0001 0.0004 0 0.0007 0 0.0002 0 0.0006 0.0001 0.0001 0 0.0013 0 0.0002 0.0001
Endoplasmic Reticulum 0.9264 0.8974 0.9184 0.8729 0.8675 0.9612 0.8812 0.9381 0.9447 0.9492 0.816 0.9422 0.7363 0.9243 0.936 0.9901 0.7759 0.8943
Endosome 0.0102 0.0056 0.0107 0.0274 0.0083 0.0032 0.0182 0.0057 0.0038 0.0029 0.0182 0.0055 0.0267 0.0008 0.0015 0.0005 0.0346 0.0074
Golgi 0.002 0.0005 0.0011 0.0031 0.001 0.0005 0.0102 0.0003 0.0015 0.0003 0.0055 0.0021 0.0183 0.0002 0.0003 0 0.0268 0.0128
Lipid Particles 0.0045 0.0037 0.0054 0.0014 0.0026 0.0013 0.0106 0.0086 0.0048 0.0009 0.0182 0.0055 0.0504 0.0063 0.0039 0.0001 0.0062 0.0168
Mitochondria 0.0003 0.0004 0.0006 0.0013 0.0008 0.0001 0.0082 0.0002 0.0016 0.0004 0.0081 0.0014 0.0016 0 0.0003 0 0.0637 0.0034
None 0.0067 0.0004 0.0015 0.0019 0.0042 0.0003 0.0018 0.0002 0.0005 0.0029 0.0128 0.0008 0.0021 0.0009 0.0255 0 0.0002 0.0002
Nuclear Periphery 0.015 0.0189 0.0143 0.0211 0.0095 0.0083 0.0125 0.0098 0.0137 0.0191 0.0342 0.0083 0.0169 0.0058 0.008 0.0037 0.0268 0.0034
Nucleolus 0.0005 0.0001 0 0.0001 0.0001 0 0.0002 0.0003 0 0.0002 0.001 0.0002 0.0062 0.0003 0.0009 0 0.0001 0.0001
Nucleus 0.0013 0.0031 0.0009 0.0038 0.0015 0.0007 0.0015 0.0027 0.0016 0.0036 0.0033 0.0039 0.0018 0.0008 0.0016 0.0001 0.0014 0.0004
Peroxisomes 0.0001 0 0.0002 0.0001 0.0009 0.0004 0.009 0 0.0051 0.0003 0.0058 0.0005 0.0196 0 0.0001 0 0.0054 0.012
Punctate Nuclear 0.0012 0.0005 0.0004 0.0014 0.0008 0.0008 0.0015 0.0002 0.0002 0.0075 0.0156 0.0003 0.0033 0.0046 0.0009 0 0.0002 0.001
Vacuole 0.0018 0.0049 0.0021 0.0026 0.0018 0.0027 0.0026 0.0043 0.0019 0.0007 0.0042 0.0032 0.0036 0.0028 0.0024 0.0015 0.0053 0.0012
Vacuole Periphery 0.0004 0.0006 0.0004 0.0012 0.0003 0.0003 0.001 0.0003 0.0007 0.0003 0.0015 0.0009 0.0007 0.0001 0.0002 0.0001 0.0023 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 176.1623 169.2791 193.2498 177.4055 126.2999 191.0781 194.4041 198.2724 198.296 153.2388
Translational Efficiency 1.0202 1.0659 1.0271 1.3619 0.9926 0.9965 1.1268 1.136 0.9646 0.97

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1669 1326 561 991 1542 1745 1141 1533 3211 3071 1702 2524

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 697.35 815.67 961.83 1078.81 762.32 852.95 1040.18 1051.62 728.55 836.85 1014.35 1062.30
Standard Deviation 118.06 150.06 121.05 152.55 104.47 123.03 139.32 150.44 116.36 136.62 138.56 151.85
Intensity Change Log 2 — 0.226103 0.463899 0.629486 — 0.162064 0.448365 0.464145 — 0.193013 0.455807 0.545502

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000098 0.000460 0.000370 0.003273 0.000111 0.000651 0.002099 0.002683 0.000105 0.000568 0.001529 0.002915
Bud Neck 0.005888 0.002759 0.003498 0.006218 0.002249 0.001887 0.002741 0.010262 0.004141 0.002263 0.002991 0.008674
Bud Site 0.010235 0.003417 0.007128 0.024342 0.002362 0.004233 0.012948 0.022483 0.006454 0.003881 0.011029 0.023213
Cell Periphery 0.001019 0.000829 0.000618 0.004894 0.000733 0.001386 0.002567 0.001539 0.000882 0.001146 0.001925 0.002856
Cytoplasm 0.477155* 0.227590* 0.214083* 0.095673 0.189015* 0.131955* 0.084671 0.168227* 0.338783* 0.173248* 0.127326* 0.139740*
Cytoplasmic Foci 0.083867 0.052626 0.042539 0.007020 0.046970 0.025496 0.007416 0.028090 0.066148 0.037210 0.018993 0.019817
Eisosomes 0.000290 0.000329 0.000144 0.000655 0.000279 0.000440 0.000172 0.000462 0.000285 0.000392 0.000163 0.000537
Endoplasmic Reticulum 0.071113 0.519286* 0.249174* 0.749769* 0.600115* 0.727331* 0.828826* 0.514154* 0.325153* 0.637501* 0.637766* 0.606663*
Endosome 0.004717 0.009067 0.015476 0.007451 0.008206 0.007194 0.006960 0.015351 0.006393 0.008003 0.009767 0.012250
Golgi 0.001427 0.005791 0.000275 0.002405 0.008887 0.010274 0.001884 0.004964 0.005009 0.008338 0.001353 0.003959
Lipid Particles 0.039397 0.036938 0.019475 0.017279 0.052023 0.027479 0.006574 0.024417 0.045460 0.031563 0.010827 0.021615
Mitochondria 0.002091 0.003515 0.000440 0.001865 0.007265 0.007569 0.000941 0.005657 0.004576 0.005818 0.000776 0.004168
Mitotic Spindle 0.001554 0.001026 0.000244 0.002054 0.000713 0.000411 0.001090 0.006743 0.001150 0.000676 0.000811 0.004902
None 0.006383 0.017387 0.000950 0.002554 0.007071 0.005219 0.001864 0.003659 0.006713 0.010473 0.001563 0.003225
Nuclear Periphery 0.010258 0.010500 0.059096 0.001177 0.008329 0.005648 0.001286 0.020516 0.009332 0.007743 0.020341 0.012923
Nuclear Periphery Foci 0.006686 0.014507 0.093563 0.017701 0.008578 0.008713 0.010586 0.031394 0.007594 0.011215 0.037936 0.026018
Nucleolus 0.001208 0.001734 0.000789 0.000107 0.000472 0.001047 0.000056 0.000646 0.000855 0.001344 0.000298 0.000435
Nucleus 0.251195* 0.060185 0.162308* 0.016918 0.030713 0.015312 0.008425 0.048785 0.145314* 0.034688 0.059147 0.036273
Peroxisomes 0.001427 0.001938 0.000653 0.000622 0.001748 0.002437 0.000323 0.001643 0.001581 0.002222 0.000432 0.001242
Vacuole 0.023565 0.028273 0.128641 0.036190 0.022388 0.013439 0.017895 0.086136 0.022999 0.019844 0.054398 0.066526
Vacuole Periphery 0.000426 0.001843 0.000533 0.001832 0.001774 0.001880 0.000676 0.002191 0.001073 0.001864 0.000629 0.002050

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.46 -6.77 -5.34 -4.67 -4.88 -2.94 -2.96 -6.56 -4.70 -0.61 -3.80 -3.17 -8.38 -6.67 -2.44
Bud Neck 3.69 2.08 -0.75 -4.40 -2.79 0.73 -1.13 -7.55 -8.17 -6.80 3.78 1.56 -5.98 -9.21 -7.34
Bud Site 3.50 0.86 -4.40 -7.22 -4.74 -2.14 -5.43 -8.65 -7.83 -3.09 2.28 -2.83 -8.43 -10.67 -5.21
Cell Periphery 0.78 1.74 -7.10 -7.91 -8.44 -3.53 -4.64 -4.60 -0.47 2.61 -1.63 -3.65 -7.82 -6.92 -2.63
Cytoplasm 30.08 23.06 51.21 15.71 10.25 8.28 14.99 1.92 -5.74 -12.05 27.92 32.28 31.77 5.09 -2.60
Cytoplasmic Foci 8.22 8.77 27.88 15.41 9.86 6.72 14.02 5.46 -1.68 -10.74 11.73 19.58 20.65 8.17 -0.68
Eisosomes -0.75 8.58 -3.53 -2.87 -4.84 -2.53 3.37 -2.27 -0.16 -3.57 -2.53 6.00 -4.05 -1.98 -5.83
Endoplasmic Reticulum -40.85* -12.85 -57.96* -14.24 -30.05* -10.65 -18.38 8.16 18.49 25.58* -33.38 -26.24 -25.05 5.22 3.76
Endosome -6.49 -10.03 -3.82 1.54 6.40 1.17 1.28 -6.24 -6.88 -6.87 -2.98 -5.35 -8.02 -5.36 -2.68
Golgi -9.28 9.75 -1.59 4.74 -3.66 -1.25 10.40 4.28 4.58 -3.41 -5.17 10.03 2.05 5.92 -4.36
Lipid Particles 1.00 9.04 8.91 7.50 0.55 7.95 16.28 9.00 1.23 -10.60 7.16 20.22 12.25 5.70 -8.16
Mitochondria -2.38 3.56 0.28 1.99 -1.86 -0.22 5.72 1.23 1.61 -4.48 -1.59 6.48 0.61 2.10 -4.79
Mitotic Spindle 0.51 1.53 -0.51 -0.99 -1.85 0.51 -0.62 -4.67 -5.16 -4.52 0.82 0.48 -4.21 -4.98 -4.79
None -4.01 8.00 3.14 5.20 -1.47 1.17 3.71 2.04 1.16 -1.57 -2.61 6.49 3.38 4.97 -2.00
Nuclear Periphery -0.18 -9.42 12.02 8.72 11.39 2.44 7.95 -5.51 -6.94 -9.49 1.93 -5.58 -2.55 -3.69 3.17
Nuclear Periphery Foci -6.23 -15.94 -6.68 -1.91 13.17 -0.10 -1.60 -9.92 -9.84 -8.39 -4.10 -13.64 -12.15 -9.34 4.31
Nucleolus -1.47 2.39 7.48 4.07 6.76 -1.17 8.31 -2.01 0.61 -5.62 -1.60 6.47 3.32 2.66 -2.40
Nucleus 34.58* 8.86 46.95* 12.45 16.48 8.06 11.66 -6.79 -12.62 -14.93 31.42 18.20 28.84 -1.81 5.44
Peroxisomes -2.40 5.60 4.10 5.40 0.07 -2.07 9.01 0.30 2.16 -7.11 -3.09 10.86 1.95 4.20 -6.37
Vacuole -2.60 -15.73 -5.98 -4.42 11.45 4.72 0.95 -15.71 -18.10 -15.78 2.42 -11.45 -16.67 -17.55 -4.15
Vacuole Periphery -5.93 -0.76 -1.46 0.06 -1.35 -0.45 5.72 -0.90 -0.56 -4.02 -4.78 3.74 -2.13 -0.24 -3.15
Standard name
Human Ortholog
Description Endoplasmic reticulum (ER) localized integral membrane protein; may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression
Localization
Cell Percentages ER (81%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Gsf2

Gsf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gsf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available