Standard name
Human Ortholog
Description Nucleosome-binding component of the SWR1 complex; SWR1 exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.14 0.14 0.14 0.2 0.1 0 0 0 0.09 0.07 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.35 0 0 0 0.14 0.16 0.11 0.51 0.34 0.44 0.35 0 0 0 0.06 0.11 0.06 0 0 0 0 0 0
Nucleus 0.89 0.88 0.93 0.93 0.95 0.86 0.87 0.87 0.74 0.77 0.82 0.8 0.9 0.81 0.81 0.71 0.69 0.84 0.83 0.81 0.76 0.66 0.71 0.53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.07 0 0 0 0.06 0.06 0.06 0.11 0.1 0.1 0.09 0.08 0 0.05 0.06 0.09 0.06 0.09 0.07 0.14 0.13 0.1 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0.05 0 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 3 2 3 2 4 1 3 0 0 0 0 0 0 2 0 1 2 1 3
Bud Neck 0 0 1 0 0 1 2 1 0 1 2 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 4 1 5 10 5 11 10 6 17 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2 10 7 13 12 16 26 7 13 4 12 5 38 38 5 7 6 0 0 1 12 22 23
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1 3 0 0 2 0 0 1
Endosome 0 0 3 2 6 5 3 0 1 0 0 1 0 5 3 2 1 0 0 1 3 1 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Mitochondria 14 47 3 4 3 64 85 70 160 111 109 148 2 5 3 2 4 4 0 2 4 0 1 2
Nucleus 102 120 361 257 405 390 471 550 233 251 205 338 75 223 218 25 24 52 97 116 312 88 235 129
Nuclear Periphery 0 0 2 0 2 0 4 1 0 1 2 1 0 0 0 0 0 0 0 0 0 0 1 4
Nucleolus 21 9 11 12 11 28 35 41 33 33 24 36 7 12 13 2 3 4 10 10 56 17 33 40
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 2 2 24 10 16 16 22 32 5 14 3 9 3 9 13 0 0 0 2 0 1 1 6 8
Vac/Vac Membrane 0 0 4 1 3 3 2 3 0 4 4 2 0 1 1 0 2 2 1 7 22 4 19 15
Unique Cell Count 114 136 387 275 428 455 544 631 313 327 250 423 83 277 268 35 35 62 118 144 412 134 330 243
Labelled Cell Count 139 180 419 298 460 527 652 732 453 442 360 567 93 294 291 38 42 71 118 144 412 134 330 243


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 4.3 6.3 5.9 6.5 5.1 5.0 5.2 4.2 4.6 4.2 4.5 5.9 6.3 6.1 6.1 7.3 6.9 7.7 7.9 8.7
Std Deviation (1e-4) 0.8 0.7 1.3 1.4 1.7 1.2 1.6 1.3 1.0 1.1 0.8 0.8 2.3 2.2 2.8 2.3 2.6 1.6 4.9 4.8 5.6
Intensity Change (Log2) – – – -0.1 0.04 -0.3 -0.33 -0.27 -0.58 -0.46 -0.58 -0.48 -0.09 -0.0 -0.05 -0.06 0.21 0.14 0.29 0.32 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2166 3.506 3.0097 2.8733 2.6626 3.2345 2.9409 4.5196 4.0648 3.4679 4.2164 3.9728 1.1508 1.8053 1.5974 1.5794 1.4627 1.5398
Actin 0.0199 0.0001 0.0017 0.0784 0 0.0141 0.0328 0 0.0026 0 0.0004 0 0.0494 0.0011 0.0099 0.0794 0.0011 0.009
Bud 0.0002 0 0.0001 0.0013 0 0.0001 0.0001 0 0.0002 0 0 0 0.0004 0.0001 0.0002 0.0005 0.0001 0.0001
Bud Neck 0.0011 0.0001 0.0001 0.0029 0.0012 0.002 0.0001 0.0001 0.0004 0.0001 0.0019 0.0009 0.003 0.0005 0.0002 0.0011 0.0014 0.0017
Bud Periphery 0.0004 0 0.0001 0.0049 0 0.0002 0.0001 0 0.0003 0 0.0001 0 0.0007 0.0001 0.0007 0.0007 0.0003 0.0001
Bud Site 0.0012 0 0.0034 0.0016 0.0001 0.0002 0.0002 0.0001 0.0005 0.0001 0.0003 0.0001 0.0057 0.0026 0.0005 0.0038 0.0005 0.0001
Cell Periphery 0.0002 0 0.0001 0.0009 0 0.0001 0 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0035 0.0002 0.0002 0.0003 0.0008 0.0001 0.0015 0.0002 0.0025 0.0001 0.0006 0.0004 0.003 0.0007 0.0015 0.0006 0.0005 0.0002
Cytoplasmic Foci 0.0164 0 0.0001 0.0058 0.0001 0.0001 0.006 0 0.0028 0 0.0004 0 0.0095 0.0051 0.0043 0.0053 0.0032 0.0008
Eisosomes 0.0006 0 0.0003 0.0008 0 0 0.0002 0 0.0001 0 0 0 0.0004 0 0.0001 0.0008 0 0
Endoplasmic Reticulum 0.0021 0 0 0.0006 0.0001 0.0001 0.0007 0 0.0013 0 0.0003 0 0.0013 0 0.0005 0.0009 0.001 0.0003
Endosome 0.0165 0 0 0.0018 0.0002 0.0004 0.0067 0 0.0054 0 0.0017 0.0001 0.0081 0.0002 0.0023 0.0085 0.0328 0.0017
Golgi 0.0097 0 0 0.0034 0 0.0005 0.0055 0 0.0012 0 0.0003 0 0.0051 0.0004 0.0015 0.0068 0.0057 0.0039
Lipid Particles 0.0161 0 0 0.0155 0 0.0001 0.0073 0 0.0028 0 0.0024 0 0.0089 0.0003 0.0026 0.0067 0.0287 0.0002
Mitochondria 0.004 0.0005 0.0045 0.0238 0.0002 0.0001 0.001 0.0001 0.002 0.0001 0.0003 0.0001 0.0034 0.0005 0.0017 0.0071 0.0017 0.0003
None 0.0089 0.0002 0.0097 0.0007 0.0001 0.0001 0.0199 0.0001 0.0054 0 0.0005 0.0026 0.0105 0.0074 0.0006 0.001 0.0005 0.0001
Nuclear Periphery 0.0074 0.0136 0.0004 0.0008 0.0053 0.0008 0.0087 0.0007 0.0084 0.0016 0.0337 0.0008 0.0096 0.0031 0.0069 0.0022 0.0188 0.0002
Nucleolus 0.0321 0.0196 0.0242 0.0176 0.0372 0.0569 0.018 0.0123 0.0197 0.0169 0.1789 0.0503 0.026 0.0334 0.0192 0.0123 0.0971 0.0681
Nucleus 0.8345 0.9645 0.9533 0.802 0.9088 0.9211 0.8753 0.9856 0.9348 0.9763 0.7316 0.9365 0.8246 0.9352 0.9369 0.855 0.7886 0.9103
Peroxisomes 0.0056 0 0.0008 0.0345 0 0.0001 0.0042 0 0.0014 0 0.0002 0 0.0146 0.0026 0.0073 0.0044 0.0012 0.0001
Punctate Nuclear 0.017 0.0009 0.0008 0.0006 0.0458 0.0019 0.0078 0.0008 0.0074 0.0045 0.0454 0.008 0.0142 0.0063 0.002 0.0017 0.0143 0.0024
Vacuole 0.0018 0.0001 0 0.0013 0.0001 0.0009 0.0017 0.0001 0.0006 0.0001 0.0006 0.0001 0.0007 0.0001 0.0005 0.0006 0.0011 0.0002
Vacuole Periphery 0.0009 0.0002 0 0.0006 0 0.0001 0.0022 0 0.0003 0 0.0004 0 0.0007 0.0001 0.0006 0.0004 0.0014 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.7801 12.8709 12.1555 12.4061 7.6367 3.9245 12.718 10.3804 12.921 13.848
Translational Efficiency 1.2082 1.2677 1.1987 0.9944 1.8344 5.0408 1.4244 1.4332 1.3459 1.3388

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1168 888 2362 295 2247 1956 2309 1055 3415 2844 4671 1350

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 800.16 896.51 945.29 972.01 750.22 903.54 891.33 952.30 767.30 901.34 918.62 956.61
Standard Deviation 91.27 111.58 116.37 133.20 97.02 113.34 105.55 144.11 98.00 112.84 114.38 142.03
Intensity Change Log 2 — 0.164031 0.240468 0.280683 — 0.268275 0.248646 0.344102 — 0.215415 0.244431 0.311719

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000191 0.001263 0.000709 0.001919 0.000178 0.000390 0.000827 0.001630 0.000182 0.000662 0.000767 0.001693
Bud Neck 0.006158 0.015683 0.032733 0.029890 0.005609 0.009229 0.039825 0.044842 0.005797 0.011244 0.036239 0.041574
Bud Site 0.000416 0.003445 0.001786 0.009283 0.000772 0.001415 0.001889 0.020219 0.000651 0.002049 0.001837 0.017829
Cell Periphery 0.000047 0.000107 0.000041 0.000573 0.000078 0.000049 0.000050 0.000238 0.000067 0.000067 0.000045 0.000311
Cytoplasm 0.003070 0.004459 0.000560 0.028262 0.006151 0.003105 0.001526 0.030936 0.005097 0.003527 0.001037 0.030352
Cytoplasmic Foci 0.000312 0.001590 0.000015 0.022484 0.001347 0.002070 0.000072 0.003109 0.000993 0.001920 0.000043 0.007343
Eisosomes 0.000017 0.000047 0.000034 0.000055 0.000019 0.000025 0.000033 0.000042 0.000019 0.000032 0.000034 0.000045
Endoplasmic Reticulum 0.001279 0.005942 0.002707 0.006308 0.001363 0.002466 0.003477 0.006260 0.001334 0.003551 0.003088 0.006270
Endosome 0.000202 0.000860 0.000156 0.026212 0.000462 0.001786 0.000482 0.008424 0.000373 0.001497 0.000317 0.012311
Golgi 0.000091 0.000781 0.000143 0.023531 0.000186 0.000967 0.000199 0.004397 0.000153 0.000909 0.000170 0.008578
Lipid Particles 0.000491 0.001311 0.000045 0.001375 0.000593 0.000574 0.000084 0.000385 0.000558 0.000804 0.000064 0.000601
Mitochondria 0.001267 0.004030 0.001645 0.008173 0.001187 0.003544 0.002119 0.006371 0.001215 0.003696 0.001880 0.006765
Mitotic Spindle 0.002608 0.005832 0.002846 0.091367 0.001972 0.005504 0.006573 0.052765 0.002190 0.005606 0.004688 0.061200
None 0.003447 0.003758 0.001638 0.031513 0.006813 0.003880 0.002373 0.012730 0.005662 0.003842 0.002001 0.016835
Nuclear Periphery 0.000676 0.000769 0.000387 0.001951 0.000582 0.000622 0.000979 0.002161 0.000614 0.000668 0.000680 0.002115
Nuclear Periphery Foci 0.000620 0.001356 0.000110 0.001847 0.000506 0.000859 0.000456 0.001324 0.000545 0.001015 0.000281 0.001438
Nucleolus 0.058722 0.081453 0.053973 0.023516 0.067074 0.062067 0.054966 0.024827 0.064218 0.068120 0.054464 0.024541
Nucleus 0.919722* 0.863092* 0.899575* 0.644515* 0.904130* 0.898115* 0.881367* 0.754533* 0.909463* 0.887180* 0.890574* 0.730492*
Peroxisomes 0.000406 0.002297 0.000323 0.005068 0.000517 0.000916 0.000336 0.001252 0.000479 0.001347 0.000329 0.002086
Vacuole 0.000110 0.001291 0.000400 0.031175 0.000306 0.001568 0.001254 0.017650 0.000239 0.001481 0.000822 0.020605
Vacuole Periphery 0.000147 0.000633 0.000172 0.010983 0.000157 0.000848 0.001113 0.005907 0.000153 0.000781 0.000637 0.007016

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.75 -20.13 -14.94 -7.48 -10.82 -6.69 -24.78 -19.83 -16.97 -10.66 -12.41 -31.53 -24.37 -16.95 -14.86
Bud Neck -6.74 -24.16 -10.91 -7.43 -1.62 -4.81 -28.23 -20.38 -18.17 -1.27 -7.97 -37.44 -22.95 -19.22 -3.45
Bud Site -2.98 -6.44 -8.78 -5.12 -7.33 -1.22 -6.14 -8.02 -7.45 -7.05 -2.94 -7.64 -9.79 -8.34 -8.53
Cell Periphery -2.98 0.41 -4.35 -2.93 -4.48 2.74 2.83 -7.82 -10.97 -11.66 -0.02 2.93 -9.11 -8.96 -11.32
Cytoplasm -0.92 3.09 -5.29 -4.47 -6.60 2.43 5.10 -5.77 -6.95 -7.96 1.50 6.25 -7.24 -7.90 -9.63
Cytoplasmic Foci -2.82 2.75 -2.16 -1.41 -2.36 -0.64 3.83 0.73 1.40 -3.87 -1.59 4.23 -1.30 0.02 -4.08
Eisosomes -11.57 -15.46 -11.22 -4.86 -7.39 -4.36 -15.67 -15.16 -12.08 -7.76 -10.89 -21.73 -18.91 -11.63 -10.46
Endoplasmic Reticulum -11.25 -15.15 -12.92 -4.87 -10.20 -6.58 -18.83 -14.80 -11.86 -9.64 -12.62 -23.45 -18.55 -11.82 -13.25
Endosome -3.18 0.57 -2.84 -2.51 -2.85 -2.80 0.41 -3.89 -2.06 -3.99 -3.41 0.94 -4.86 -3.22 -4.99
Golgi -1.94 -7.09 -2.52 -2.10 -2.46 -2.24 -0.82 -2.31 -1.03 -2.23 -2.86 -1.34 -3.38 -2.08 -3.29
Lipid Particles -1.73 1.51 -1.23 0.87 -3.94 0.19 4.03 0.79 0.75 -10.36 -1.30 3.81 -0.21 1.44 -9.03
Mitochondria -2.59 -1.19 -3.92 -0.40 -3.56 -3.50 -5.43 -6.57 -1.43 -4.46 -4.34 -3.85 -7.45 -1.42 -5.75
Mitotic Spindle -1.78 -1.95 -6.46 -6.10 -6.25 -2.66 -4.68 -10.56 -9.75 -9.09 -3.18 -5.03 -12.36 -11.49 -11.34
None -0.16 2.77 -3.13 -3.10 -3.82 3.27 5.87 -1.46 -4.28 -6.32 2.90 6.72 -3.41 -5.38 -7.55
Nuclear Periphery -0.47 0.71 -7.09 -9.13 -11.41 -0.33 -2.17 -10.28 -9.37 -6.78 -0.52 -1.00 -11.60 -11.54 -11.01
Nuclear Periphery Foci -1.93 1.09 -1.76 0.44 -3.80 -0.94 0.99 -2.33 -1.44 -3.00 -1.79 1.55 -2.67 -1.18 -3.73
Nucleolus -5.23 -1.59 9.99 13.92 12.35 1.77 1.31 13.81 12.17 12.21 -1.46 0.26 16.46 17.42 16.74
Nucleus 8.66 8.12 10.96 7.28 8.75 1.20 9.30 16.08 14.99 11.21 5.73 11.51 19.71 16.27 14.98
Peroxisomes -4.05 0.20 -2.24 1.08 -2.40 -1.87 1.41 -1.55 0.55 -3.47 -4.20 1.25 -2.74 1.61 -4.07
Vacuole -3.84 -8.21 -5.78 -5.57 -5.71 -3.40 -4.60 -9.18 -8.58 -8.80 -4.58 -5.91 -10.84 -10.23 -10.57
Vacuole Periphery -2.65 -1.06 -1.98 -1.53 -1.95 -3.76 -1.97 -2.96 -1.92 -2.00 -4.52 -2.08 -3.51 -2.41 -2.94
Standard name
Human Ortholog
Description Nucleosome-binding component of the SWR1 complex; SWR1 exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Vps71

Vps71


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps71-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available