Standard name
Human Ortholog
Description Inner nuclear membrane protein; highly enriched at telomeres and subtelomeric regions; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance; SEC1 has a paralog, HEH2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.14 0.19 0.16 0.11 0.1 0.07 0.06 0.07 0.08 0.09 0.12 0.16 0.09 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0
Endosome 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.08 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0.23 0.15 0.27 0.21 0.4 0.4 0.53 0.57 0.59 0.42 0.22 0.12 0.1 0.1 0 0.06 0 0.06 0.12 0.11 0.13 0.13
Nucleus 0.07 0.06 0 0.15 0.14 0.22 0.31 0.38 0.32 0.28 0.4 0.1 0.06 0 0.1 0.12 0.07 0 0 0 0 0 0
Nuclear Periphery 0.27 0.28 0.08 0.15 0.19 0.21 0.18 0.23 0.19 0.25 0.17 0.31 0.33 0.32 0.38 0.3 0.37 0.84 0.7 0.65 0.58 0.5 0.46
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.66 0.71 0.69 0.38 0.46 0.35 0.26 0.19 0.21 0.17 0.21 0.52 0.66 0.59 0.58 0.63 0.66 0 0.09 0.08 0.11 0.14 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 1 1 2 5 6
Bud 0 0 0 1 1 1 9 8 7 3 10 0 0 1 0 0 0 1 3 2 1 11 8
Bud Neck 0 0 0 0 0 0 4 0 0 0 2 0 0 0 0 0 0 0 0 1 0 1 3
Bud Site 1 0 0 0 0 1 3 4 5 7 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 8 12 30 28 30 23 40 28 27 26 29 34 51 82 22 17 12 1 6 5 7 5 6
Endoplasmic Reticulum 5 3 2 1 2 1 2 1 2 1 0 2 2 10 12 8 6 2 4 11 9 21 21
Endosome 14 8 13 6 5 2 4 0 1 1 5 4 19 11 17 17 15 7 9 6 6 10 30
Golgi 0 2 0 0 1 0 0 0 0 0 0 0 0 0 2 1 0 0 0 1 1 4 1
Mitochondria 51 66 31 39 39 84 160 226 254 208 157 81 53 50 23 6 12 7 21 34 28 54 63
Nucleus 14 18 7 22 25 46 122 160 144 98 150 35 25 20 23 25 13 1 11 3 8 11 11
Nuclear Periphery 54 80 17 21 35 44 73 98 83 87 64 112 142 160 90 64 69 212 230 190 155 210 229
Nucleolus 0 1 0 0 0 0 0 2 3 5 5 1 4 4 10 16 8 0 1 0 1 4 5
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1
SpindlePole 1 0 3 0 0 0 0 0 2 2 5 2 1 2 4 4 6 2 6 6 7 13 15
Vac/Vac Membrane 133 202 146 55 85 74 103 80 95 60 78 192 286 293 138 133 122 9 29 22 28 57 82
Unique Cell Count 202 283 212 144 184 211 398 423 445 353 377 367 434 497 238 212 185 253 331 292 266 421 497
Labelled Cell Count 282 392 250 173 223 276 521 607 623 500 510 463 583 633 342 291 264 253 331 292 266 421 497


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.8 5.7 5.2 5.5 4.9 4.8 4.4 4.2 4.0 4.5 4.6 4.7 4.7 8.1 8.5 8.4 4.6 4.9 5.1
Std Deviation (1e-4) 1.3 0.9 1.7 1.9 1.4 1.6 1.4 1.1 0.9 1.2 0.9 0.9 1.0 1.2 1.9 1.9 2.0 1.5 1.8 1.6
Intensity Change (Log2) -0.13 -0.04 -0.22 -0.23 -0.38 -0.43 -0.52 -0.32 -0.3 -0.28 -0.28 0.5 0.58 0.56 -0.31 -0.22 -0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole -2.4 -1.3 -0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0364 3.6823 3.5234 3.4565 2.9577 3.6962 1.8415 1.48 1.5146 0.9961 0.6995 1.5164 0.6742 0.899 0.3634 0.9553 0.5583 0.8023
Actin 0.0487 0.0003 0.0102 0.0292 0.0008 0.0089 0.036 0.0002 0.0074 0.0003 0.0002 0.0053 0.0156 0.0005 0.0022 0.0004 0.0541 0.0034
Bud 0.0005 0 0.0001 0.0003 0 0 0.0004 0 0.0003 0 0 0.0001 0.0103 0.0096 0.0091 0 0.0032 0.0067
Bud Neck 0.0025 0.0001 0.0015 0.0029 0.0002 0.0002 0.0073 0 0.0001 0 0.0001 0.0005 0.0019 0.0002 0.0004 0 0.0012 0.0046
Bud Periphery 0.0007 0 0.0001 0.0004 0.0001 0.0001 0.0006 0 0.0007 0 0.0001 0.0002 0.0237 0.0161 0.0165 0.0001 0.0203 0.026
Bud Site 0.0052 0.0002 0.001 0.0127 0 0.0001 0.0082 0.0003 0.0008 0 0.0001 0.0001 0.0027 0.0122 0.0095 0 0.0059 0.0008
Cell Periphery 0.0003 0 0.0002 0.0003 0.0001 0 0.0003 0 0.0001 0 0.0001 0 0.0098 0.0031 0.005 0.0001 0.0091 0.0079
Cytoplasm 0.009 0.0073 0.0111 0.0065 0.0052 0.0013 0.0018 0.0047 0.0027 0.0012 0.0004 0.0006 0.0052 0.0117 0.0035 0.0004 0.0023 0.0065
Cytoplasmic Foci 0.0177 0.0003 0.0048 0.0196 0.0002 0.0008 0.019 0.0008 0.0043 0 0.0015 0.0012 0.0082 0.0011 0.0043 0 0.0041 0.0054
Eisosomes 0.0005 0 0.0004 0.0004 0 0 0.0003 0 0.0001 0 0 0.0001 0.0004 0.0005 0.0007 0 0.0004 0.0001
Endoplasmic Reticulum 0.0214 0.0279 0.0478 0.0963 0.0489 0.0101 0.0056 0.0216 0.0149 0.0089 0.0056 0.0075 0.0115 0.0171 0.0115 0.027 0.0046 0.0065
Endosome 0.0257 0.0032 0.0057 0.0279 0.0056 0.0025 0.0213 0.0031 0.0075 0.0012 0.0165 0.0056 0.0152 0.0035 0.0038 0.0021 0.0126 0.0064
Golgi 0.008 0.0001 0.0032 0.0071 0.0001 0.0007 0.013 0.0001 0.0054 0 0.0033 0.0044 0.0055 0.0001 0.0003 0.0001 0.0054 0.0017
Lipid Particles 0.0303 0.001 0.0032 0.0322 0.001 0.0015 0.023 0.0007 0.0051 0.0002 0.0512 0.0032 0.0105 0.0004 0.0087 0.0003 0.0133 0.0037
Mitochondria 0.0083 0.0015 0.0135 0.009 0.0066 0.0018 0.0061 0.0013 0.0104 0.0013 0.0041 0.0102 0.0048 0.0017 0.0064 0.0027 0.0103 0.0077
None 0.0195 0.0043 0.0307 0.0033 0.0042 0.0128 0.0042 0.0072 0.0034 0.0003 0.0004 0.0003 0.0807 0.0561 0.0737 0.0005 0.054 0.0548
Nuclear Periphery 0.6912 0.8123 0.7768 0.672 0.8037 0.8717 0.7915 0.8529 0.8694 0.8984 0.871 0.9232 0.7252 0.76 0.7872 0.8994 0.7202 0.7973
Nucleolus 0.0025 0.0012 0.0012 0.0007 0.0008 0.0009 0.0009 0.0009 0.0006 0.0001 0.0004 0.0005 0.0009 0.0009 0.0011 0.0001 0.0028 0.0027
Nucleus 0.0406 0.1014 0.0521 0.0414 0.0969 0.0631 0.0162 0.065 0.0194 0.0568 0.0178 0.0171 0.0292 0.0611 0.0207 0.0211 0.0318 0.0241
Peroxisomes 0.0152 0.0001 0.002 0.013 0.0001 0.0013 0.0054 0 0.0054 0 0.0006 0.0007 0.007 0.0001 0.0005 0 0.0067 0.0038
Punctate Nuclear 0.0289 0.0079 0.0063 0.0054 0.0013 0.0028 0.0113 0.0025 0.007 0.0007 0.001 0.0018 0.0045 0.0133 0.0089 0.0002 0.0079 0.003
Vacuole 0.0029 0.0029 0.0029 0.0044 0.0029 0.0013 0.0037 0.0032 0.0021 0.001 0.0039 0.0013 0.0058 0.0032 0.0031 0.0019 0.0072 0.0105
Vacuole Periphery 0.0204 0.0279 0.0254 0.0151 0.0209 0.0181 0.0237 0.0353 0.0329 0.0294 0.0217 0.0162 0.0212 0.0275 0.023 0.0435 0.0228 0.0162

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.893 41.3451 39.1533 28.0179 15.7053 18.0497 47.9751 52.3991 44.6747 19.32
Translational Efficiency 0.916 0.9798 1.0697 1.2447 0.8349 0.6064 0.9923 1.0895 0.9189 0.8301

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
121 2031 213 1453 1658 1326 2084 1641 1779 3357 2297 3094

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 633.63 791.35 1008.01 848.27 722.98 742.78 896.56 877.80 716.90 772.17 906.89 863.93
Standard Deviation 65.34 106.23 107.11 145.97 77.24 93.85 100.20 130.42 79.73 104.26 105.91 138.72
Intensity Change Log 2 0.320675 0.669797 0.420883 0.038979 0.310444 0.279937 0.177415 0.489459 0.347485

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000030 0.000064 0.001700 0.000285 0.000105 0.000215 0.000156 0.000303 0.000100 0.000124 0.000299 0.000295
Bud Neck 0.000825 0.001016 0.008302 0.003790 0.000634 0.001251 0.001790 0.006401 0.000647 0.001109 0.002394 0.005175
Bud Site 0.000069 0.000520 0.002819 0.002135 0.000302 0.000596 0.000588 0.002905 0.000286 0.000550 0.000795 0.002544
Cell Periphery 0.000083 0.000041 0.000092 0.000065 0.000077 0.000075 0.000032 0.000082 0.000078 0.000054 0.000037 0.000074
Cytoplasm 0.155856* 0.076477 0.004962 0.033737 0.098874 0.049762 0.020535 0.029833 0.102749* 0.065924 0.019091 0.031666
Cytoplasmic Foci 0.091251 0.014134 0.000688 0.001826 0.027772 0.023584 0.000561 0.002395 0.032090 0.017867 0.000572 0.002128
Eisosomes 0.000094 0.000014 0.000039 0.000011 0.000034 0.000028 0.000010 0.000014 0.000038 0.000019 0.000013 0.000012
Endoplasmic Reticulum 0.016927 0.050002 0.067673 0.019597 0.055061 0.072395 0.017560 0.013172 0.052467 0.058847 0.022207 0.016189
Endosome 0.008482 0.005265 0.003350 0.005982 0.004998 0.009229 0.001930 0.005930 0.005235 0.006831 0.002062 0.005954
Golgi 0.000495 0.000888 0.000126 0.001340 0.000682 0.002739 0.000037 0.000393 0.000669 0.001619 0.000045 0.000838
Lipid Particles 0.146868* 0.038420 0.002713 0.002199 0.104741* 0.096207 0.001573 0.002339 0.107606* 0.061245 0.001678 0.002274
Mitochondria 0.006401 0.010125 0.003464 0.002942 0.007165 0.020005 0.002548 0.004025 0.007113 0.014027 0.002633 0.003517
Mitotic Spindle 0.000082 0.007822 0.013707 0.068618 0.005036 0.006199 0.008543 0.065670 0.004699 0.007181 0.009022 0.067054
None 0.008615 0.005052 0.000654 0.002676 0.006451 0.004003 0.001453 0.005263 0.006598 0.004638 0.001379 0.004048
Nuclear Periphery 0.217681* 0.533577* 0.317454* 0.451385* 0.447867* 0.472837* 0.597226* 0.410719* 0.432210* 0.509585* 0.571283* 0.429817*
Nuclear Periphery Foci 0.048031 0.162154 0.207949* 0.236157* 0.098848 0.160018 0.125932 0.294213* 0.095392 0.161310 0.133538 0.266949*
Nucleolus 0.021179 0.007577 0.042064 0.015983 0.008546 0.008295 0.019387 0.026430 0.009405 0.007861 0.021490 0.021524
Nucleus 0.253917* 0.074521 0.294928* 0.131751* 0.117866 0.054017 0.192252* 0.110470 0.127119 0.066422 0.201774* 0.120464
Peroxisomes 0.003548 0.000671 0.000949 0.000336 0.001734 0.002810 0.000183 0.000667 0.001857 0.001516 0.000254 0.000512
Vacuole 0.017476 0.009032 0.025152 0.014893 0.011161 0.010464 0.007071 0.015279 0.011590 0.009598 0.008747 0.015098
Vacuole Periphery 0.002088 0.002630 0.001214 0.004291 0.002047 0.005270 0.000633 0.003494 0.002050 0.003673 0.000687 0.003869

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.45 -11.92 -9.11 -8.00 9.79 -1.05 -1.33 -4.31 -2.31 -6.00 -0.35 -3.92 -4.31 -6.21 -0.90
Bud Neck -0.69 -4.22 -6.59 -6.12 2.72 -1.78 -6.16 -10.16 -8.60 -7.47 -2.16 -7.32 -12.25 -10.71 -5.69
Bud Site -2.02 -2.19 -3.79 -2.79 0.73 -1.38 -2.02 -4.78 -4.30 -3.82 -1.41 -2.80 -5.78 -5.09 -3.61
Cell Periphery 4.49 -2.21 1.37 -4.16 3.79 0.24 5.71 -1.19 -1.21 -5.70 3.31 5.05 -0.26 -3.79 -5.66
Cytoplasm 4.31 8.39 6.66 9.28 -9.96 9.73 17.41 13.19 3.42 -4.05 7.84 19.18 15.28 9.82 -5.91
Cytoplasmic Foci 5.70 6.71 6.63 11.20 -4.71 1.63 15.28 13.59 10.26 -4.19 6.55 16.30 15.29 14.73 -5.57
Eisosomes 7.05 3.97 7.27 2.23 8.42 2.71 11.20 7.65 3.87 -3.92 9.70 11.10 12.14 3.40 0.63
Endoplasmic Reticulum -10.58 -11.12 -1.15 13.37 11.81 -4.55 15.38 17.18 17.89 3.84 -2.43 12.83 16.10 22.59 5.81
Endosome 1.58 2.06 1.22 -1.00 -2.35 -3.82 6.84 -1.60 2.38 -5.34 -2.86 7.02 -1.67 1.12 -7.26
Golgi -1.17 2.40 -1.53 -0.74 -2.34 -3.60 4.65 1.68 4.12 -3.49 -3.14 4.81 -0.54 2.28 -3.23
Lipid Particles 6.98 9.37 9.41 18.30 1.64 1.43 26.25 26.00 21.70 -2.66 10.57 27.76 27.60 27.52 -2.18
Mitochondria -2.66 2.30 2.80 7.60 1.81 -6.32 6.17 3.73 8.11 -3.86 -6.09 6.37 4.97 10.78 -3.22
Mitotic Spindle -6.32 -3.52 -13.95 -12.09 -7.04 -0.67 -2.89 -14.12 -13.87 -12.75 -1.84 -3.66 -18.60 -18.24 -16.32
None 1.43 3.36 2.45 2.76 -3.92 2.56 5.75 0.76 -1.61 -4.54 2.20 6.32 2.53 0.52 -5.09
Nuclear Periphery -12.11 -2.20 -8.30 8.25 -7.60 -2.22 -10.90 5.69 7.72 16.96 -7.83 -10.31 2.44 12.27 14.44
Nuclear Periphery Foci -11.55 -8.06 -16.44 -8.09 -2.20 -8.99 -4.42 -21.52 -12.62 -17.55* -12.82 -6.33 -25.52 -14.90 -18.36
Nucleolus 2.17 -2.21 0.65 -5.82 3.89 0.55 -6.82 -9.12 -9.21 -3.37 1.54 -7.38 -8.63 -11.07 -0.51
Nucleus 7.64 -2.01 4.85 -8.58 8.43 11.41 -11.27 -1.90 -11.92 8.69 11.62 -11.66 -1.10 -13.85 11.08
Peroxisomes 3.42 3.15 3.81 2.75 4.57 -1.57 4.49 2.30 3.16 -3.31 0.84 4.93 3.69 3.44 -3.19
Vacuole 2.33 -3.31 -0.24 -4.90 3.46 0.60 -0.71 -5.03 -5.49 -4.20 1.87 -2.18 -5.39 -7.51 -2.74
Vacuole Periphery -1.08 2.00 -2.56 -1.93 -4.01 -4.81 6.26 -2.81 2.46 -6.35 -4.20 6.41 -3.75 -0.19 -7.34