Standard name
Human Ortholog
Description Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; phosphorylated by Cdc28p; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 – 0 0 0.07 0.07 0.06 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.71 0.75 0.71 0.7 0.62 0.6 0.5 0.5 0.41 0.48 0.39 0.43 0.74 0.78 0.91 – 0.74 0.65 0.5 0.58 0.37 0.88 0.88 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.14 0.05 0.18 0.19 0.38 0.54 0.4 0.56 0.59 0.72 0.65 0 0 0 – 0.06 0 0.06 0 0.14 0 0 0
Nucleus 0.18 0.17 0.23 0.19 0.28 0.24 0.23 0.26 0.24 0.2 0.18 0.18 0.24 0.18 0 – 0.15 0.11 0.21 0.2 0.26 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.07 0 0.07 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 – 0.09 0.17 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 3 1 3 1 3 2
Bud 1 1 0 4 4 4 26 10 12 18 21 12 0 0 0 – 0 0 21 7 18 1 6 9
Bud Neck 0 0 0 0 0 1 0 5 2 0 0 0 0 0 0 – 0 0 2 0 2 0 1 2
Bud Site 2 0 0 0 3 0 5 11 2 13 9 8 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 1 2 0 4 1 0 3 1 1 1 0 0 0 – 0 1 1 0 1 0 3 3
Cytoplasm 227 80 228 211 177 275 331 335 159 240 149 225 156 294 364 – 63 30 160 61 123 157 326 370
Endoplasmic Reticulum 3 0 2 2 1 1 0 0 0 1 1 1 0 0 0 – 6 3 0 0 0 0 0 1
Endosome 0 1 1 1 0 0 0 3 0 0 0 0 0 3 8 – 1 1 3 1 4 1 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0
Mitochondria 60 15 17 54 53 173 360 266 217 293 275 338 0 1 1 – 5 1 19 3 47 3 4 10
Nucleus 57 18 73 58 79 108 150 172 91 101 68 95 51 67 17 – 13 5 66 21 85 3 0 1
Nuclear Periphery 0 0 0 1 1 2 1 3 1 0 0 1 0 0 0 – 0 0 0 0 0 0 0 2
Nucleolus 1 2 1 2 4 4 11 9 14 10 8 4 0 0 0 – 1 0 1 0 2 0 1 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0
SpindlePole 1 0 3 2 1 3 8 14 4 6 9 7 0 0 2 – 1 0 23 4 22 1 6 8
Vac/Vac Membrane 11 4 12 9 11 5 7 9 7 5 5 8 7 20 22 – 8 8 12 3 11 2 2 3
Unique Cell Count 318 106 319 303 284 455 666 668 386 495 382 522 211 377 400 85 46 324 107 331 179 371 432
Labelled Cell Count 363 121 338 346 334 580 901 837 512 688 546 700 214 385 415 98 50 324 107 331 179 371 432


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 3.9 4.1 3.9 4.0 3.2 3.1 3.1 3.1 2.8 2.6 2.6 5.3 5.2 5.1 – 5.9 6.4 5.2 5.0 5.3
Std Deviation (1e-4) 0.7 0.9 1.4 1.9 1.9 1.1 1.1 1.2 1.4 1.1 1.3 1.0 1.3 1.0 1.5 – 2.0 2.6 1.7 1.5 2.0
Intensity Change (Log2) – – – -0.07 -0.02 -0.37 -0.4 -0.39 -0.41 -0.53 -0.63 -0.64 0.37 0.35 0.33 – 0.53 0.66 0.34 0.29 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.45 1.0705 0.6513 0.0765 0.0465 0.3633 -0.5067 0.7498 0.6245 -0.0449 -0.453 -0.3284 -1.5769 -0.1526 -0.4383 -0.9535 -1.3099 -1.373
Actin 0.0187 0.0002 0.0002 0.0076 0.0031 0.003 0.0285 0.0006 0.0093 0.0258 0.0071 0.0032 0.0155 0.0003 0.0106 0.0258 0.0187 0.0034
Bud 0.0135 0.0078 0.002 0.0018 0.0016 0.002 0.0171 0.013 0.0025 0.0064 0.0211 0.0047 0.0183 0.0014 0.0028 0.0031 0.0007 0.0048
Bud Neck 0.0033 0.0007 0.0004 0.0003 0.0027 0.006 0.0144 0.0014 0.0043 0.0036 0.0021 0.0049 0.0075 0.0006 0.0013 0.0012 0.0009 0.0062
Bud Periphery 0.0149 0.0072 0.0013 0.0038 0.0012 0.0034 0.0235 0.0122 0.0034 0.0073 0.0143 0.0099 0.0236 0.0008 0.0034 0.0019 0.0008 0.0066
Bud Site 0.017 0.0099 0.0017 0.0006 0.0144 0.0005 0.0267 0.0196 0.0028 0.0091 0.0019 0.0009 0.0344 0.0055 0.0028 0.0023 0.0011 0.0015
Cell Periphery 0.0018 0.0002 0.0001 0.0001 0.0002 0.0003 0.0027 0.0008 0.0003 0.0006 0.0003 0.0004 0.0035 0.0002 0.0002 0.0001 0.0001 0.0004
Cytoplasm 0.0505 0.0282 0.063 0.1179 0.0833 0.0628 0.0709 0.0672 0.0635 0.251 0.2085 0.1058 0.0602 0.0865 0.1139 0.1609 0.2918 0.1439
Cytoplasmic Foci 0.0356 0.0029 0.0023 0.0104 0.0064 0.0064 0.0173 0.0056 0.0088 0.0215 0.018 0.0139 0.0403 0.0016 0.0077 0.0247 0.0149 0.017
Eisosomes 0.0005 0 0 0.0002 0.0002 0.0001 0.0003 0 0.0001 0.0002 0.0001 0.0001 0.0003 0 0.0001 0.0002 0.0002 0.0001
Endoplasmic Reticulum 0.0037 0.0006 0.0008 0.0025 0.0014 0.002 0.0054 0.0022 0.0014 0.0021 0.0032 0.0021 0.0107 0.0024 0.0015 0.0013 0.003 0.003
Endosome 0.0153 0.0004 0.0014 0.0279 0.0044 0.0023 0.0243 0.0019 0.0159 0.0345 0.0257 0.0064 0.0409 0.0015 0.0065 0.0228 0.0329 0.0081
Golgi 0.0034 0 0 0.0097 0.001 0.0005 0.0089 0.0002 0.0066 0.0156 0.005 0.0041 0.0163 0 0.0036 0.0143 0.017 0.0024
Lipid Particles 0.0097 0.0001 0.0001 0.0072 0.0011 0.0004 0.0205 0.0024 0.0147 0.0123 0.015 0.0057 0.0181 0.0002 0.0039 0.0143 0.0206 0.0044
Mitochondria 0.0077 0.0008 0.0004 0.0144 0.0013 0.0011 0.0173 0.0011 0.0183 0.0088 0.0289 0.0124 0.0423 0.0004 0.0083 0.0612 0.0205 0.0095
None 0.3672 0.2383 0.2758 0.6848 0.6072 0.4219 0.3494 0.225 0.1289 0.3557 0.4842 0.4477 0.3158 0.236 0.1338 0.4733 0.4896 0.3828
Nuclear Periphery 0.0101 0.0047 0.0069 0.0198 0.0043 0.0095 0.0233 0.0077 0.0115 0.0071 0.0117 0.0056 0.0156 0.0143 0.0079 0.0027 0.0066 0.0083
Nucleolus 0.045 0.0097 0.0062 0.0012 0.0142 0.0289 0.04 0.0102 0.0077 0.0065 0.0047 0.0128 0.041 0.0068 0.0067 0.0012 0.0007 0.0127
Nucleus 0.3083 0.652 0.6196 0.0631 0.2078 0.4178 0.2426 0.6067 0.6599 0.1976 0.1087 0.3318 0.2018 0.6299 0.6612 0.172 0.0377 0.3558
Peroxisomes 0.0137 0.0003 0.0001 0.0021 0.0009 0.0002 0.0234 0.0019 0.007 0.0059 0.0138 0.003 0.0086 0 0.0074 0.0115 0.0096 0.0041
Punctate Nuclear 0.0351 0.0341 0.0162 0.0207 0.0415 0.0271 0.0106 0.0163 0.0266 0.0159 0.0186 0.0173 0.0243 0.0096 0.0136 0.003 0.0285 0.0182
Vacuole 0.0218 0.0015 0.0009 0.0021 0.0016 0.0029 0.0271 0.0035 0.0048 0.0104 0.0048 0.0056 0.0526 0.0016 0.0022 0.0016 0.0029 0.0052
Vacuole Periphery 0.003 0.0003 0.0002 0.0019 0.0003 0.0007 0.0058 0.0004 0.0015 0.0022 0.0021 0.0019 0.0086 0.0003 0.0005 0.0007 0.0012 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.9206 19.5912 6.3207 25.0396 30.8854 45.7117 12.9187 6.3941 15.0196 44.5288
Translational Efficiency 2.0187 1.7189 1.4792 1.5181 2.8456 2.3841 1.7016 2.2851 1.6943 1.8738

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2081 179 123 1044 1419 1776 125 707 3500 1955 248 1751

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 727.44 776.18 967.60 916.96 675.24 731.23 1036.02 1006.81 706.28 735.35 1002.09 953.24
Standard Deviation 99.85 104.72 125.30 153.18 89.63 96.31 144.94 159.38 99.21 97.97 139.81 161.84
Intensity Change Log 2 — 0.093563 0.411583 0.334031 — 0.114925 0.617580 0.576319 — 0.103886 0.514423 0.455750

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000162 0.001121 0.001036 0.000958 0.000120 0.000483 0.001324 0.002343 0.000145 0.000541 0.001181 0.001517
Bud Neck 0.017571 0.033784 0.004700 0.014045 0.011032 0.023352 0.004910 0.015536 0.014920 0.024307 0.004806 0.014647
Bud Site 0.007531 0.025078 0.013898 0.014954 0.003641 0.011696 0.002320 0.023723 0.005954 0.012922 0.008062 0.018495
Cell Periphery 0.000685 0.000347 0.000309 0.000320 0.000475 0.000494 0.000271 0.000344 0.000600 0.000481 0.000290 0.000330
Cytoplasm 0.351934* 0.241626* 0.129032* 0.210249* 0.377419* 0.353706* 0.073134 0.164505* 0.362266* 0.343444* 0.100858* 0.191779*
Cytoplasmic Foci 0.088952 0.212336* 0.022576 0.006344 0.107026 0.111891 0.005976 0.006971 0.096280 0.121088 0.014209 0.006597
Eisosomes 0.000080 0.000135 0.000141 0.000037 0.000089 0.000095 0.000096 0.000041 0.000084 0.000099 0.000118 0.000039
Endoplasmic Reticulum 0.001329 0.002887 0.009671 0.005003 0.001154 0.001529 0.012103 0.005310 0.001258 0.001653 0.010896 0.005127
Endosome 0.004520 0.022536 0.007014 0.003523 0.004523 0.009945 0.002164 0.002640 0.004521 0.011098 0.004569 0.003166
Golgi 0.001263 0.002624 0.000391 0.003441 0.000659 0.001288 0.000116 0.000794 0.001018 0.001411 0.000252 0.002372
Lipid Particles 0.004413 0.005277 0.007086 0.000809 0.006089 0.005791 0.001766 0.000660 0.005092 0.005744 0.004404 0.000749
Mitochondria 0.002699 0.006183 0.000986 0.003682 0.002961 0.005192 0.001028 0.003281 0.002805 0.005283 0.001007 0.003520
Mitotic Spindle 0.002686 0.005294 0.001071 0.051131 0.001124 0.003217 0.001872 0.026552 0.002053 0.003407 0.001475 0.041207
None 0.022381 0.004626 0.003274 0.016165 0.021190 0.016797 0.004626 0.009875 0.021899 0.015683 0.003956 0.013625
Nuclear Periphery 0.000628 0.000568 0.001670 0.001766 0.000526 0.000840 0.001698 0.001603 0.000587 0.000816 0.001684 0.001700
Nuclear Periphery Foci 0.000485 0.000950 0.001536 0.000817 0.000439 0.000445 0.000978 0.001432 0.000466 0.000491 0.001255 0.001065
Nucleolus 0.009184 0.005193 0.005684 0.002126 0.005800 0.007291 0.003453 0.001081 0.007812 0.007098 0.004560 0.001704
Nucleus 0.466907* 0.345050* 0.725379* 0.618549* 0.438470* 0.401851* 0.842735* 0.690368* 0.455378* 0.396651* 0.784530* 0.647547*
Peroxisomes 0.002425 0.025785 0.001258 0.000531 0.002374 0.006235 0.000426 0.004660 0.002404 0.008025 0.000839 0.002198
Vacuole 0.013566 0.057544 0.062713 0.039043 0.014443 0.035961 0.038813 0.037992 0.013921 0.037937 0.050666 0.038619
Vacuole Periphery 0.000599 0.001055 0.000575 0.006508 0.000443 0.001901 0.000191 0.000286 0.000536 0.001823 0.000382 0.003996

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.38 -6.80 -10.44 0.07 1.87 -2.07 -10.33 -5.17 -3.15 0.76 -2.42 -11.74 -9.29 -3.49 2.06
Bud Neck -2.71 6.60 2.16 3.26 -5.07 -5.93 2.75 -2.04 3.97 -5.00 -5.18 7.40 0.26 5.40 -7.35
Bud Site -2.93 -0.53 -1.31 2.49 0.24 -5.83 -0.83 -4.22 -0.73 -3.96 -4.96 -0.49 -3.88 0.84 -0.88
Cell Periphery 3.87 6.85 9.54 1.31 2.77 -0.24 4.28 4.57 4.31 0.07 2.36 8.29 10.50 5.12 2.22
Cytoplasm 5.56 14.16 20.17 3.99 -2.67 2.18 21.00 23.32 22.64 -4.09 2.34 24.21 30.07 23.46 -4.89
Cytoplasmic Foci -6.62 12.83 23.54 11.53 3.80 -0.64 21.92 22.71 25.11 1.10 -4.77 23.77 32.62 27.73 3.88
Eisosomes -4.42 -6.11 7.79 6.75 8.43 -0.96 -3.50 6.37 6.45 6.38 -2.99 -7.01 10.06 9.40 10.62
Endoplasmic Reticulum -3.01 -8.17 -14.80 -5.23 4.15 -1.82 -10.73 -14.48 -13.04 5.95 -2.33 -13.29 -20.57 -16.96 7.18
Endosome -4.34 -1.01 5.95 4.93 2.30 -5.76 2.60 5.30 9.25 2.44 -7.45 -0.19 7.97 10.55 2.83
Golgi -1.67 2.66 0.05 1.28 -1.21 -3.28 5.23 1.77 4.27 -1.34 -1.43 3.79 0.32 1.09 -1.43
Lipid Particles -1.16 -1.38 7.97 4.26 4.00 0.47 5.49 7.90 7.77 4.86 -0.93 0.99 11.22 8.53 4.81
Mitochondria -1.24 3.17 0.26 1.31 -4.37 -2.24 2.31 0.97 4.20 -2.24 -3.34 3.89 0.90 4.51 -4.91
Mitotic Spindle -1.51 1.57 -7.49 -6.45 -7.90 -2.30 -1.56 -6.43 -5.89 -2.03 -1.65 -1.27 -9.78 -9.24 -5.61
None 10.28 14.59 7.41 -3.39 -6.25 2.27 8.83 7.61 6.83 -3.32 4.41 15.86 10.44 5.19 -6.48
Nuclear Periphery 0.17 -8.69 -15.19 -13.90 -3.64 -3.47 -6.99 -16.49 -11.79 0.06 -2.80 -9.92 -21.79 -15.12 -1.69
Nuclear Periphery Foci -1.64 -4.43 -4.71 0.81 2.60 0.18 -4.43 -5.13 -5.05 0.84 -0.60 -6.24 -6.96 -4.96 2.63
Nucleolus 3.28 2.17 10.98 2.96 2.15 -1.56 3.71 7.65 9.46 2.03 1.16 3.93 13.34 9.25 2.68
Nucleus 4.38 -10.12 -16.31 -11.29 1.88 2.84 -13.37 -16.60 -19.86 3.34 5.77 -16.45 -23.09 -25.58 3.76
Peroxisomes -4.09 2.90 5.78 4.50 2.74 -4.09 5.56 1.17 4.09 -1.19 -5.63 5.60 4.08 7.28 0.07
Vacuole -5.28 -5.97 -12.81 -0.43 0.90 -9.61 -5.54 -11.77 -8.51 -0.22 -11.87 -8.09 -17.33 -9.94 0.63
Vacuole Periphery -2.23 0.21 -1.81 -1.36 -1.81 -3.10 5.00 5.05 3.63 0.41 -3.00 1.38 -1.63 0.50 -1.88
Standard name
Human Ortholog
Description Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; phosphorylated by Cdc28p; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (41%), cytoplasm (17%), mixed (28%)
Cell Cycle Regulation Yes
cytoplasm - MA (1.4e-05)
Subcompartmental Group N/A

Yox1

Yox1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yox1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available