Standard name | |
---|---|
Human Ortholog | |
Description | Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 90 | 73 | 75 | 64 | 88 | 105 | 182 | 122 | 89 | 107 | 86 | 81 | 153 | 206 | 175 | 25 | 16 | 16 | 12 | 25 | 24 | 24 | 46 | 36 |
Bud | 15 | 19 | 16 | 22 | 37 | 30 | 50 | 47 | 40 | 58 | 52 | 53 | 30 | 19 | 12 | 5 | 0 | 4 | 1 | 1 | 2 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 14 | 24 | 11 | 8 | 8 |
Bud Site | 2 | 3 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 122 | 148 | 177 | 211 | 188 | 206 | 265 | 116 | 143 | 143 | 92 | 144 | 248 | 345 | 385 | 86 | 60 | 42 | 171 | 172 | 220 | 119 | 331 | 406 |
Cytoplasm | 26 | 68 | 86 | 108 | 171 | 150 | 234 | 201 | 170 | 204 | 191 | 224 | 60 | 80 | 50 | 21 | 19 | 28 | 5 | 19 | 17 | 3 | 1 | 1 |
Endoplasmic Reticulum | 18 | 11 | 11 | 4 | 15 | 23 | 31 | 28 | 16 | 11 | 14 | 20 | 18 | 12 | 29 | 8 | 2 | 5 | 35 | 29 | 39 | 9 | 9 | 29 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 6 | 3 | 10 | 2 | 1 | 2 | 1 | 2 | 4 |
Mitochondria | 14 | 3 | 0 | 2 | 17 | 37 | 75 | 37 | 97 | 108 | 109 | 63 | 7 | 25 | 23 | 7 | 7 | 6 | 0 | 7 | 22 | 6 | 8 | 8 |
Nucleus | 0 | 0 | 0 | 1 | 2 | 0 | 3 | 5 | 1 | 2 | 3 | 0 | 3 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 59 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 5 | 3 | 2 | 1 | 6 | 7 | 2 | 7 | 6 | 7 | 0 | 0 | 1 | 3 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 14 | 1 | 2 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 4 | 6 | 4 | 1 | 3 |
Unique Cell Count | 194 | 230 | 283 | 306 | 390 | 382 | 580 | 433 | 371 | 429 | 403 | 427 | 330 | 465 | 469 | 124 | 96 | 79 | 239 | 279 | 367 | 183 | 414 | 505 |
Labelled Cell Count | 288 | 325 | 372 | 415 | 520 | 552 | 848 | 588 | 559 | 645 | 564 | 593 | 522 | 751 | 681 | 163 | 116 | 118 | 239 | 279 | 367 | 183 | 414 | 505 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 8.6 | 8.3 | 7.9 | 8.0 | 7.1 | 7.5 | 8.0 | 6.6 | 6.4 | 6.2 | 6.9 | 8.9 | 9.5 | 9.6 | 13.1 | 12.8 | 12.4 | 8.2 | 8.6 | 9.8 |
Std Deviation (1e-4) | 1.2 | 1.4 | 1.0 | 0.9 | 1.4 | 1.0 | 1.0 | 1.0 | 1.1 | 1.0 | 1.0 | 0.8 | 1.1 | 1.3 | 1.2 | 2.7 | 2.2 | 3.0 | 1.0 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.06 | -0.24 | -0.16 | -0.07 | -0.33 | -0.38 | -0.43 | -0.28 | 0.09 | 0.19 | 0.2 | 0.65 | 0.61 | 0.57 | -0.02 | 0.05 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.6 | -1.2 | 0.3 | 1.5 | 0.5 | -0.7 | -0.5 | -1.6 | -2.4 | 5.1 | 4.9 | 3.0 | -1.4 | -1.9 | -1.1 |
Bud | 0.8 | 1.8 | 1.1 | 1.5 | 2.4 | 2.3 | 3.4 | 3.1 | 3.0 | 1.6 | -1.0 | -2.2 | -0.7 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.6 | -3.7 | -2.2 | -4.7 | -9.5 | -6.1 | -7.7 | -10.5 | -7.6 | 3.4 | 3.4 | 6.0 | 1.3 | 0 | -1.5 |
Cytoplasm | 1.3 | 3.5 | 2.4 | 2.8 | 4.3 | 4.0 | 4.6 | 4.5 | 5.8 | -3.5 | -4.2 | -6.8 | -2.8 | -2.0 | 0.9 |
Endoplasmic Reticulum | -2.0 | 0 | 1.2 | 0.9 | 1.5 | 0.3 | -1.0 | -0.3 | 0.5 | 0.9 | -1.0 | 1.4 | 1.1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 3.6 | 5.4 | 6.3 | 5.0 | 9.3 | 9.2 | 9.5 | 6.8 | 0 | 4.0 | 3.8 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.2 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0083 | 4.4087 | 4.1478 | 4.0074 | 2.982 | 4.2759 | 3.7693 | 4.7344 | 4.564 | 4.5442 | 3.5631 | 4.5792 | 5.0664 | 6.0837 | 5.3747 | 5.7626 | 5.1961 | 5.6741 |
Actin | 0.0304 | 0.0248 | 0.0315 | 0.0248 | 0.0963 | 0.0358 | 0.0619 | 0.0277 | 0.0234 | 0.0038 | 0.0196 | 0.0175 | 0.033 | 0.0146 | 0.035 | 0.0124 | 0.012 | 0.0136 |
Bud | 0.0008 | 0.001 | 0.0017 | 0.0028 | 0.0011 | 0.0009 | 0.0026 | 0.0005 | 0.0018 | 0.0014 | 0.0009 | 0.0007 | 0.0008 | 0.0076 | 0.0025 | 0.0026 | 0.0008 | 0.0006 |
Bud Neck | 0.005 | 0.0053 | 0.0165 | 0.0082 | 0.0288 | 0.0401 | 0.0083 | 0.0072 | 0.0144 | 0.0103 | 0.0146 | 0.0222 | 0.0061 | 0.0073 | 0.0178 | 0.0179 | 0.0202 | 0.0517 |
Bud Periphery | 0.0084 | 0.0062 | 0.0133 | 0.0647 | 0.0146 | 0.0129 | 0.0126 | 0.0027 | 0.0156 | 0.0352 | 0.013 | 0.0137 | 0.0061 | 0.0045 | 0.0191 | 0.0185 | 0.0126 | 0.0115 |
Bud Site | 0.0177 | 0.0854 | 0.0501 | 0.0036 | 0.004 | 0.0036 | 0.0267 | 0.1201 | 0.0665 | 0.0016 | 0.0042 | 0.002 | 0.0144 | 0.094 | 0.0358 | 0.0071 | 0.0035 | 0.0018 |
Cell Periphery | 0.5562 | 0.4369 | 0.5855 | 0.6217 | 0.5606 | 0.5856 | 0.5683 | 0.5669 | 0.6534 | 0.7408 | 0.5747 | 0.7574 | 0.6336 | 0.5587 | 0.6023 | 0.7148 | 0.597 | 0.671 |
Cytoplasm | 0.2032 | 0.3244 | 0.2331 | 0.2079 | 0.1928 | 0.2307 | 0.1276 | 0.2368 | 0.143 | 0.1213 | 0.1744 | 0.1052 | 0.1737 | 0.2626 | 0.179 | 0.0682 | 0.2096 | 0.169 |
Cytoplasmic Foci | 0.0024 | 0.0054 | 0.004 | 0.0006 | 0.0044 | 0.0023 | 0.0145 | 0.0009 | 0.0022 | 0.0013 | 0.0124 | 0.001 | 0.008 | 0.0007 | 0.0136 | 0.0055 | 0.0082 | 0.001 |
Eisosomes | 0.0534 | 0.0221 | 0.015 | 0.0091 | 0.0199 | 0.0228 | 0.0445 | 0.0102 | 0.023 | 0.0155 | 0.0342 | 0.0197 | 0.0162 | 0.0093 | 0.007 | 0.0466 | 0.002 | 0.0048 |
Endoplasmic Reticulum | 0.0687 | 0.0513 | 0.0278 | 0.0451 | 0.0118 | 0.0392 | 0.0347 | 0.0135 | 0.0302 | 0.048 | 0.0179 | 0.0453 | 0.0504 | 0.0294 | 0.0343 | 0.0666 | 0.0432 | 0.0549 |
Endosome | 0.0055 | 0.0042 | 0.0013 | 0.0003 | 0.011 | 0.0016 | 0.0121 | 0.0003 | 0.0028 | 0.0058 | 0.0121 | 0.0031 | 0.0041 | 0.0002 | 0.0128 | 0.0038 | 0.0047 | 0.0027 |
Golgi | 0.0072 | 0.009 | 0.0049 | 0.0019 | 0.01 | 0.003 | 0.0151 | 0.0019 | 0.0049 | 0.0018 | 0.0215 | 0.0041 | 0.0047 | 0.0011 | 0.0063 | 0.0083 | 0.0045 | 0.0032 |
Lipid Particles | 0.0055 | 0.006 | 0.0037 | 0.0016 | 0.0214 | 0.0031 | 0.0219 | 0.0034 | 0.0027 | 0.0025 | 0.0257 | 0.0029 | 0.0093 | 0.0035 | 0.0096 | 0.0026 | 0.0468 | 0.0064 |
Mitochondria | 0.0053 | 0.0006 | 0.0007 | 0.0006 | 0.0112 | 0.0004 | 0.0157 | 0.0001 | 0.007 | 0.0005 | 0.0567 | 0.0005 | 0.0077 | 0.0003 | 0.0022 | 0.0142 | 0.0014 | 0.0004 |
None | 0.0119 | 0.0123 | 0.0032 | 0.0009 | 0.0056 | 0.0074 | 0.0052 | 0.0038 | 0.001 | 0.004 | 0.0004 | 0.0005 | 0.0032 | 0.0006 | 0.001 | 0.0005 | 0.0012 | 0.0004 |
Nuclear Periphery | 0.0052 | 0.001 | 0.0008 | 0.0008 | 0.0004 | 0.0055 | 0.0049 | 0.001 | 0.0011 | 0.0019 | 0.0005 | 0.0008 | 0.0097 | 0.0016 | 0.0018 | 0.0018 | 0.0033 | 0.0022 |
Nucleolus | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0001 |
Nucleus | 0.0063 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.001 | 0.0026 | 0.0003 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0009 | 0.0004 | 0.0007 | 0.0007 | 0.0002 | 0.0003 |
Peroxisomes | 0.0003 | 0.0001 | 0.003 | 0 | 0.0031 | 0.0001 | 0.0128 | 0 | 0.003 | 0 | 0.0126 | 0.0002 | 0.0034 | 0 | 0.0114 | 0.0019 | 0.0183 | 0.0004 |
Punctate Nuclear | 0.0005 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.009 | 0.0001 | 0.0006 | 0.0003 | 0.0078 | 0.0009 |
Vacuole | 0.0047 | 0.0033 | 0.003 | 0.0049 | 0.0019 | 0.0032 | 0.0051 | 0.0023 | 0.0031 | 0.0034 | 0.0026 | 0.0025 | 0.0043 | 0.0033 | 0.0062 | 0.0048 | 0.002 | 0.0032 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0008 | 0 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0009 | 0 | 0.0004 | 0.0004 | 0.0003 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.7797 | 19.4523 | 15.4907 | 22.1483 | 21.3319 | 18.0351 | 25.1849 | 27.3589 | 23.154 | 30.7844 |
Translational Efficiency | 1.107 | 1.0935 | 1.0651 | 0.6096 | 0.7243 | 1.4151 | 0.9494 | 0.8299 | 0.974 | 0.923 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2101 | 912 | 762 | 863 | 869 | 160 | 1347 | 351 | 2970 | 1072 | 2109 | 1214 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 936.52 | 1059.20 | 1545.66 | 1256.93 | 972.97 | 1044.33 | 1191.63 | 1348.18 | 947.18 | 1056.98 | 1319.54 | 1283.31 |
Standard Deviation | 105.88 | 160.05 | 171.28 | 202.02 | 117.72 | 119.31 | 146.95 | 194.54 | 110.72 | 154.75 | 230.90 | 204.12 |
Intensity Change Log 2 | — | 0.177593 | 0.722841 | 0.424523 | — | 0.102110 | 0.292469 | 0.470546 | — | 0.139625 | 0.519561 | 0.448157 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003628 | 0.010364 | 0.054982 | 0.018140 | 0.005970 | 0.010080 | 0.072227 | 0.047687 | 0.004313 | 0.010322 | 0.065996 | 0.026683 |
Bud Neck | 0.005957 | 0.011593 | 0.027824 | 0.010647 | 0.010848 | 0.012114 | 0.025582 | 0.021040 | 0.007388 | 0.011671 | 0.026392 | 0.013652 |
Bud Site | 0.013333 | 0.039968 | 0.047767 | 0.064854 | 0.021803 | 0.028329 | 0.083598 | 0.103430* | 0.015812 | 0.038230 | 0.070652 | 0.076008 |
Cell Periphery | 0.634560* | 0.689041* | 0.521813* | 0.742319* | 0.707850* | 0.686029* | 0.343114* | 0.553442* | 0.656004* | 0.688591* | 0.407679* | 0.687710* |
Cytoplasm | 0.149012* | 0.141082* | 0.025992 | 0.082120 | 0.192263* | 0.185261* | 0.192836* | 0.115394* | 0.161667* | 0.147676* | 0.132554* | 0.091740 |
Cytoplasmic Foci | 0.009524 | 0.008076 | 0.000828 | 0.005531 | 0.003192 | 0.006389 | 0.027285 | 0.003859 | 0.007671 | 0.007824 | 0.017726 | 0.005047 |
Eisosomes | 0.010907 | 0.010060 | 0.034375 | 0.005906 | 0.008409 | 0.015301 | 0.036829 | 0.013343 | 0.010176 | 0.010843 | 0.035943 | 0.008056 |
Endoplasmic Reticulum | 0.030273 | 0.030876 | 0.143570* | 0.019058 | 0.015909 | 0.014349 | 0.067919 | 0.023820 | 0.026070 | 0.028410 | 0.095252 | 0.020435 |
Endosome | 0.002408 | 0.004325 | 0.003588 | 0.004285 | 0.001488 | 0.002418 | 0.011108 | 0.004037 | 0.002139 | 0.004041 | 0.008391 | 0.004213 |
Golgi | 0.002317 | 0.004048 | 0.002802 | 0.004628 | 0.002307 | 0.003048 | 0.021916 | 0.006575 | 0.002314 | 0.003899 | 0.015010 | 0.005191 |
Lipid Particles | 0.010269 | 0.007160 | 0.085788 | 0.017844 | 0.002862 | 0.005109 | 0.062990 | 0.071237 | 0.008102 | 0.006854 | 0.071227 | 0.033281 |
Mitochondria | 0.003365 | 0.002372 | 0.000351 | 0.001528 | 0.000697 | 0.001688 | 0.001548 | 0.000649 | 0.002584 | 0.002270 | 0.001115 | 0.001274 |
Mitotic Spindle | 0.000989 | 0.000622 | 0.000987 | 0.000785 | 0.000332 | 0.000162 | 0.000532 | 0.000805 | 0.000797 | 0.000553 | 0.000696 | 0.000791 |
None | 0.000206 | 0.000071 | 0.000122 | 0.000066 | 0.000032 | 0.000022 | 0.000158 | 0.000110 | 0.000155 | 0.000064 | 0.000145 | 0.000079 |
Nuclear Periphery | 0.001384 | 0.000643 | 0.001584 | 0.000781 | 0.000419 | 0.000226 | 0.000444 | 0.000556 | 0.001102 | 0.000580 | 0.000856 | 0.000716 |
Nuclear Periphery Foci | 0.000309 | 0.000557 | 0.001866 | 0.000821 | 0.000497 | 0.000380 | 0.002141 | 0.001819 | 0.000364 | 0.000530 | 0.002041 | 0.001110 |
Nucleolus | 0.000052 | 0.000111 | 0.000017 | 0.000037 | 0.000021 | 0.000016 | 0.000059 | 0.000014 | 0.000043 | 0.000097 | 0.000044 | 0.000030 |
Nucleus | 0.000793 | 0.003572 | 0.002462 | 0.000847 | 0.000777 | 0.000540 | 0.004447 | 0.001353 | 0.000788 | 0.003120 | 0.003730 | 0.000993 |
Peroxisomes | 0.117455* | 0.027536 | 0.004515 | 0.003755 | 0.017984 | 0.022646 | 0.014499 | 0.005801 | 0.088350 | 0.026807 | 0.010892 | 0.004347 |
Vacuole | 0.002788 | 0.006198 | 0.038480 | 0.014036 | 0.006053 | 0.005620 | 0.030163 | 0.024355 | 0.003744 | 0.006112 | 0.033168 | 0.017020 |
Vacuole Periphery | 0.000468 | 0.001723 | 0.000287 | 0.002013 | 0.000290 | 0.000270 | 0.000605 | 0.000673 | 0.000416 | 0.001506 | 0.000490 | 0.001626 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.72 | -12.90 | -6.23 | -2.89 | 8.06 | -1.38 | -18.45 | -8.03 | -6.54 | 4.02 | -4.57 | -23.37 | -9.86 | -6.41 | 11.49 |
Bud Neck | -3.85 | -14.63 | -4.66 | 0.56 | 10.15 | -0.58 | -7.94 | -4.29 | -3.26 | 1.81 | -3.28 | -16.67 | -6.25 | -1.33 | 9.32 |
Bud Site | -6.83 | -10.11 | -11.46 | -4.32 | -3.11 | -0.83 | -13.29 | -8.67 | -6.34 | -2.02 | -6.35 | -18.01 | -14.24 | -7.11 | -1.10 |
Cell Periphery | -4.45 | 9.87 | -9.10 | -3.77 | -15.97 | 0.92 | 28.25* | 7.81 | 4.78 | -10.35 | -2.99 | 28.21 | -2.93 | 0.08 | -24.21 |
Cytoplasm | 1.10 | 26.59 | 10.50 | 7.52 | -10.34 | 0.41 | -0.08 | 6.76 | 3.91 | 7.47 | 2.09 | 5.40 | 12.43 | 7.80 | 6.93 |
Cytoplasmic Foci | 0.88 | 17.34 | 2.97 | 1.66 | -3.76 | -1.25 | -13.05 | -0.88 | 0.90 | 11.87 | -0.37 | -8.20 | 2.60 | 2.12 | 8.52 |
Eisosomes | 0.90 | -11.52 | 7.46 | 4.23 | 13.91 | -2.39 | -15.71 | -2.94 | 0.63 | 10.37 | -0.72 | -19.16 | 3.12 | 2.76 | 19.76 |
Endoplasmic Reticulum | -0.16 | -15.01 | 3.87 | 3.06 | 16.21 | 0.39 | -13.31 | -2.26 | -2.12 | 10.20 | -0.76 | -18.41 | 2.47 | 2.45 | 19.06 |
Endosome | -2.26 | -6.61 | -2.02 | 0.05 | -0.74 | -1.07 | -9.63 | -2.34 | -1.25 | 5.12 | -2.57 | -10.16 | -2.83 | -0.14 | 4.43 |
Golgi | -2.23 | -1.55 | -1.78 | -0.36 | -1.43 | -0.89 | -13.47 | -1.55 | -1.27 | 5.09 | -2.35 | -13.70 | -2.36 | -0.92 | 6.59 |
Lipid Particles | 2.08 | -14.38 | -3.21 | -4.17 | 12.03 | -1.36 | -17.54 | -7.49 | -7.15 | -0.83 | 0.98 | -21.51 | -7.87 | -7.92 | 8.91 |
Mitochondria | 2.31 | 14.15 | 4.44 | 1.63 | -3.29 | -1.08 | -2.69 | 0.25 | 1.12 | 2.52 | 0.83 | 5.91 | 4.36 | 2.31 | -0.49 |
Mitotic Spindle | 1.63 | 0.03 | 2.60 | -0.48 | 2.49 | 5.44 | -3.00 | -3.52 | -4.78 | -1.86 | 1.18 | 1.60 | 0.07 | -1.13 | -1.37 |
None | 5.68 | 3.52 | 7.17 | 0.41 | 3.18 | 1.72 | -3.97 | -1.37 | -1.52 | 0.79 | 4.91 | 0.27 | 3.50 | -0.59 | 2.47 |
Nuclear Periphery | 8.80 | -1.82 | 6.86 | -1.51 | 6.96 | 5.09 | -0.60 | -1.61 | -3.98 | -1.31 | 7.69 | 4.21 | 5.71 | -1.80 | 2.10 |
Nuclear Periphery Foci | -2.07 | -22.17 | -4.84 | -0.94 | 8.25 | 2.28 | -11.21 | -1.94 | -2.11 | 0.43 | -1.74 | -17.38 | -3.51 | -2.03 | 3.95 |
Nucleolus | -0.92 | 19.68 | 2.01 | 1.11 | -2.67 | 1.99 | -3.97 | 3.12 | 1.12 | 4.82 | -0.97 | -0.28 | 2.38 | 1.17 | 1.80 |
Nucleus | -1.64 | -10.54 | -0.84 | 1.61 | 9.57 | 0.97 | -9.11 | -1.56 | -4.47 | 9.87 | -1.60 | -14.72 | -1.75 | 1.47 | 14.38 |
Peroxisomes | 20.88* | 31.28* | 31.81* | 9.43 | 0.77 | -1.01 | 1.81 | 6.45 | 3.86 | 6.92 | 17.77 | 28.10 | 30.90 | 10.05 | 7.91 |
Vacuole | -5.64 | -24.09 | -12.10 | -7.08 | 14.04 | 0.59 | -14.14 | -5.90 | -6.05 | 1.04 | -4.23 | -25.35 | -11.50 | -8.86 | 9.49 |
Vacuole Periphery | -1.53 | 5.64 | -2.26 | -0.28 | -2.53 | 0.26 | -2.25 | -1.65 | -1.69 | -0.25 | -1.56 | -0.83 | -2.46 | -0.15 | -2.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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