Standard name
Human Ortholog
Description Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and in concert with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; interacts with DNA upstream of RNAPII and the non-template strand of the transcription bubble; Spt5p is the only transcription elongation factor conserved in all domains of life
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0.05 0 0.05 0.09 0.08 0 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0.07 0.09 – 0.12 0.09 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.86 0.85 0.82 0.83 0.86 0.73 0.71 0.61 0.61 – 0.62 0.56 0.8 0.76 0.71 0.83 0.71 0.75 0.69 0.79 0.63 0.75 0.6 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.59 0.55 0.64 0.42 0.27 0.49 0.53 0.55 0.62 – 0.51 0.62 0.64 0.4 0.44 0.33 0.36 0.33 0.27 0.18 0.3 0.21 0.31 0.4
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 1 1 0 2 – 0 0 0 0 1 0 0 0 1 1 1 0 5 5
Bud Neck 0 0 0 0 0 0 0 2 0 – 0 1 1 1 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 2 0 4 4 4 5 – 0 2 1 3 7 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 1 3 2 7 2 11 8 5 – 8 9 4 34 29 7 14 17 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 4 1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 1 0 0 3 3 1 2 1 1 2 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Mitochondria 1 3 2 7 4 8 15 34 24 – 20 28 1 2 14 5 4 15 0 0 1 0 0 2
Nucleus 154 211 144 226 221 159 256 292 160 – 100 180 66 288 273 137 115 172 133 199 116 129 202 178
Nuclear Periphery 0 0 0 0 2 1 3 3 3 – 3 2 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 106 138 112 115 69 106 191 266 163 – 82 199 53 152 170 55 58 75 51 45 56 35 104 150
Peroxisomes 0 0 0 0 1 0 1 1 0 – 0 0 1 1 8 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 1 0 0 – 1 0 0 5 16 0 0 0 1 0 0 0 2 3
Vac/Vac Membrane 0 2 0 1 1 2 1 6 1 – 1 2 1 2 3 4 4 7 2 3 4 4 16 24
Unique Cell Count 180 249 175 271 258 218 362 482 261 162 321 83 380 385 165 161 230 192 254 185 172 335 374
Labelled Cell Count 262 355 262 356 305 283 484 616 363 215 424 128 488 526 212 200 289 192 254 185 172 335 374


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.6 24.5 16.3 16.4 15.8 13.7 12.7 12.5 11.5 – 12.4 11.3 20.5 21.6 17.4 20.5 23.7 22.7 23.8 23.5 23.9
Std Deviation (1e-4) 7.4 7.0 4.0 4.8 7.0 3.4 3.7 4.3 3.7 – 6.2 4.0 7.0 9.5 7.5 8.4 10.3 9.8 7.8 8.7 9.0
Intensity Change (Log2) – – – 0.01 -0.05 -0.25 -0.37 -0.39 -0.5 – -0.4 -0.53 0.33 0.41 0.1 0.33 0.54 0.47 0.55 0.52 0.55


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 1.7 0 0 3.2 2.7 0 2.9 2.6
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 1.9 2.9 3.5 – 4.2 3.4 0 0 1.6 0 0 2.7
Nucleus 0.3 0.9 -2.2 -2.9 -5.2 -4.7 – -4.2 -5.9 -0.5 -1.7 -2.9 0.2 -2.4 -1.8
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus -4.4 -7.7 -3.0 -2.5 -2.0 -0.3 – -2.5 -0.4 0 -5.3 -4.4 -5.7 -5.1 -6.3
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 2.7 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 13.666 18.6907 15.9258 14.1987 12.7109 16.1861 19.9045 23.3857 20.6885 19.1076 19.7875 20.6811 17.459 17.7696 16.7738 16.3126 15.8207 17.0973
Actin 0.0917 0.0034 0.0065 0.0001 0.0767 0 0.0004 0.0046 0.0067 0.0809 0.0008 0.0003 0.0009 0 0 0 0 0
Bud 0.002 0.0001 0.0003 0.0002 0.001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0 0 0 0 0 0
Bud Neck 0.004 0.0004 0.0007 0.0001 0.0031 0.0002 0.0001 0.0001 0.0001 0.0003 0.0003 0.0002 0.0001 0 0 0 0.0001 0.0001
Bud Periphery 0.0021 0.0003 0.0002 0.0002 0.0012 0.0001 0.0001 0.0002 0.0001 0.0003 0.0004 0.0001 0 0 0 0 0.0001 0
Bud Site 0.0134 0.0154 0.0004 0.0005 0.01 0.0001 0.0005 0.0004 0.0003 0.0012 0.0004 0.0001 0.0003 0 0 0 0.0001 0
Cell Periphery 0.0008 0.0011 0.0001 0.0004 0.0005 0.0002 0.0003 0 0.0001 0 0.0005 0.0001 0 0 0 0 0.0001 0
Cytoplasm 0.0052 0.0171 0.0075 0.0002 0.0064 0.0003 0.0142 0 0.0048 0 0.001 0.0001 0 0 0 0 0.0001 0
Cytoplasmic Foci 0.0329 0 0.0047 0.0121 0.0038 0.0034 0.002 0.0017 0.004 0.001 0.0016 0.0002 0.009 0 0 0 0 0
Eisosomes 0.0008 0.0002 0 0.0001 0.0004 0 0 0 0.0002 0.0002 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0068 0.0001 0.0028 0 0.0003 0 0.0008 0 0.0064 0.0001 0.0007 0.0002 0 0 0 0 0 0
Endosome 0.0378 0.0003 0.0054 0.0002 0.0027 0.0001 0.004 0.0017 0.0085 0.0008 0.0178 0.0022 0.0008 0 0 0 0 0
Golgi 0.0218 0.0003 0.0018 0.0001 0.0091 0 0.0001 0.0045 0.0026 0.0037 0.0011 0.0007 0.0014 0 0 0 0 0
Lipid Particles 0.0382 0.0001 0.0037 0.0111 0.0013 0.0014 0.0004 0.0004 0.002 0.0009 0.0036 0.0007 0.0118 0 0 0 0 0
Mitochondria 0.0237 0.011 0.014 0.0065 0.0063 0.0021 0.0046 0.0026 0.0024 0.0037 0.0467 0.0013 0.001 0.0002 0.0003 0.0008 0.0009 0.0004
None 0.0041 0.0001 0.0006 0.0007 0.0822 0.0003 0.0044 0 0.0007 0.0001 0.002 0.0001 0.0001 0 0 0 0 0
Nuclear Periphery 0.0079 0.0004 0.0053 0.0004 0.0011 0.0001 0.0097 0.0001 0.0069 0 0.0091 0.0026 0 0.0001 0 0 0.0003 0.0002
Nucleolus 0.166 0.1463 0.0867 0.1922 0.4634 0.3652 0.1755 0.0828 0.05 0.0813 0.469 0.2642 0.0967 0.0844 0.1151 0.0498 0.5195 0.268
Nucleus 0.5094 0.8029 0.8444 0.7687 0.3163 0.623 0.7738 0.8946 0.9014 0.8232 0.4329 0.7184 0.8403 0.9152 0.8845 0.9491 0.4778 0.7309
Peroxisomes 0.0097 0.0001 0.0028 0.0008 0.0012 0.0001 0.0002 0.0059 0.0006 0.0019 0.0008 0.0001 0.037 0 0 0 0 0
Punctate Nuclear 0.0157 0 0.0109 0.0027 0.0125 0.0011 0.0069 0.0001 0.0013 0.0001 0.0034 0.0073 0.0004 0 0 0 0.0005 0.0001
Vacuole 0.0031 0.0002 0.0005 0.001 0.0004 0.0007 0.0008 0.0001 0.0003 0 0.0035 0.0005 0 0 0 0 0.0002 0
Vacuole Periphery 0.0029 0.0002 0.0008 0.0016 0.0003 0.0016 0.0008 0.0001 0.0007 0.0001 0.0042 0.0007 0 0.0001 0 0.0001 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 64.469 61.185 69.2494 81.2894 81.57 71.1703 69.2846 72.7533 82.7781 79.9809
Translational Efficiency 3.0145 3.0636 2.3099 2.337 2.257 2.2594 1.8322 2.0049 1.6981 1.7088

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
78 141 1719 1 516 409 407 98 594 550 2126 99

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1603.98 1874.42 2004.78 262.99 1169.86 1333.81 1549.42 2220.64 1226.87 1472.40 1917.61 2200.87
Standard Deviation 413.58 466.74 440.84 — 250.91 338.86 270.38 576.52 314.08 443.80 450.81 606.08
Intensity Change Log 2 — 0.224788 0.321788 -2.608576 — 0.189217 0.405392 0.924640 — 0.209893 0.357640 -0.159434

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000430 0.000655 0.000701 0.000227 0.000376 0.000950 0.000892 0.000593 0.000383 0.000874 0.000737 0.000589
Bud Neck 0.001165 0.002480 0.005208 0.000012 0.003124 0.004720 0.010112 0.010088 0.002867 0.004146 0.006147 0.009986
Bud Site 0.000198 0.001148 0.000602 0.000028 0.000245 0.002440 0.000991 0.001142 0.000239 0.002108 0.000677 0.001131
Cell Periphery 0.000015 0.000153 0.000043 0.000203 0.000040 0.000150 0.000034 0.000030 0.000036 0.000151 0.000042 0.000032
Cytoplasm 0.000033 0.002701 0.000043 0.000033 0.000135 0.000994 0.000124 0.000031 0.000121 0.001432 0.000059 0.000031
Cytoplasmic Foci 0.000195 0.003379 0.000305 0.000012 0.000032 0.000670 0.000170 0.000313 0.000054 0.001365 0.000279 0.000310
Eisosomes 0.000107 0.000178 0.000235 0.001665 0.000079 0.000067 0.000135 0.000116 0.000083 0.000095 0.000216 0.000132
Endoplasmic Reticulum 0.000131 0.000184 0.000165 0.000001 0.000644 0.001497 0.000700 0.000177 0.000576 0.001160 0.000267 0.000175
Endosome 0.000004 0.001045 0.000010 0.000000 0.000028 0.001260 0.000021 0.000146 0.000024 0.001205 0.000012 0.000144
Golgi 0.000014 0.000116 0.000038 0.000010 0.000029 0.000808 0.000071 0.000302 0.000027 0.000631 0.000044 0.000299
Lipid Particles 0.000001 0.000063 0.000004 0.000039 0.000008 0.000358 0.000016 0.000025 0.000007 0.000283 0.000006 0.000025
Mitochondria 0.000768 0.001389 0.001405 0.000017 0.000516 0.002620 0.001635 0.007501 0.000549 0.002304 0.001449 0.007426
Mitotic Spindle 0.000003 0.000040 0.000158 0.000156 0.000067 0.000759 0.000066 0.001603 0.000058 0.000574 0.000141 0.001588
None 0.000888 0.000981 0.000524 0.997025* 0.000822 0.001398 0.000960 0.000482 0.000830 0.001291 0.000608 0.010548
Nuclear Periphery 0.000011 0.000026 0.000048 0.000000 0.000055 0.000087 0.000061 0.000112 0.000049 0.000072 0.000050 0.000111
Nuclear Periphery Foci 0.000002 0.000020 0.000007 0.000001 0.000847 0.000251 0.000007 0.000056 0.000736 0.000191 0.000007 0.000055
Nucleolus 0.065379 0.152545 0.097557 0.000002 0.021158 0.022361 0.061945 0.030703 0.026965 0.055735 0.090739 0.030393
Nucleus 0.925245* 0.810877* 0.882756* 0.000013 0.970519* 0.954482* 0.915874* 0.940184* 0.964574* 0.917667* 0.889096* 0.930687*
Peroxisomes 0.005212 0.019772 0.009571 0.000556 0.001030 0.001582 0.005632 0.003822 0.001579 0.006245 0.008817 0.003789
Vacuole 0.000070 0.001914 0.000331 0.000000 0.000154 0.001858 0.000397 0.000976 0.000143 0.001873 0.000344 0.000967
Vacuole Periphery 0.000129 0.000333 0.000290 0.000000 0.000093 0.000689 0.000156 0.001599 0.000098 0.000598 0.000264 0.001583

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN -2.30 -16.49 -3.38 1.22 3.70 -2.70 -15.83 -3.27 1.30 1.47
Bud Neck NaN NaN NaN NaN NaN -1.81 -11.92 -2.20 -1.57 0.13 -2.04 -13.47 -2.27 -1.73 -1.18
Bud Site NaN NaN NaN NaN NaN -2.27 -8.36 -2.20 1.25 -0.16 -2.64 -11.23 -2.21 1.35 -1.08
Cell Periphery NaN NaN NaN NaN NaN -1.41 0.30 0.54 1.52 0.41 -1.61 -0.42 0.23 1.63 0.61
Cytoplasm NaN NaN NaN NaN NaN -1.50 0.09 1.30 1.56 1.52 -1.82 0.87 1.30 1.89 1.71
Cytoplasmic Foci NaN NaN NaN NaN NaN -2.01 -2.76 -1.87 1.01 -0.99 -2.30 -3.52 -1.75 1.75 -0.32
Eisosomes NaN NaN NaN NaN NaN 0.71 -3.69 -1.77 -2.74 1.02 -0.87 -8.98 -1.97 -1.53 3.36
Endoplasmic Reticulum NaN NaN NaN NaN NaN -1.96 -2.53 8.44 2.94 10.67 -1.82 7.37 7.68 2.94 2.44
Endosome NaN NaN NaN NaN NaN -1.63 0.29 -1.15 1.48 -1.22 -1.90 0.86 -1.19 1.71 -1.34
Golgi NaN NaN NaN NaN NaN -1.28 -3.06 -1.07 0.78 -0.87 -1.34 -1.41 -1.07 0.67 -1.00
Lipid Particles NaN NaN NaN NaN NaN -1.94 -1.60 -0.86 1.84 -0.31 -2.12 0.15 -0.95 1.98 -1.04
Mitochondria NaN NaN NaN NaN NaN -2.28 -18.32 -1.20 -0.80 -0.99 -2.58 -18.40 -1.19 -0.86 -1.02
Mitotic Spindle NaN NaN NaN NaN NaN -1.53 -0.12 -1.01 -0.50 -1.01 -1.52 -1.18 -1.02 -0.64 -0.95
None NaN NaN NaN NaN NaN -1.43 -0.41 1.31 2.55 1.87 -1.44 1.02 -0.97 -0.92 -0.99
Nuclear Periphery NaN NaN NaN NaN NaN -0.71 -0.28 -0.81 -0.22 -0.78 -0.68 -0.06 -0.92 -0.45 -1.01
Nuclear Periphery Foci NaN NaN NaN NaN NaN 0.67 1.00 0.95 1.85 -1.09 0.71 1.00 0.94 1.76 -1.10
Nucleolus NaN NaN NaN NaN NaN -0.41 -7.88 -1.57 -1.35 2.78 -5.47 -16.85 -0.85 2.26 6.51
Nucleus NaN NaN NaN NaN NaN 2.60 9.74 2.24 0.94 -1.24 6.51 17.69 2.12 -0.60 -2.10
Peroxisomes NaN NaN NaN NaN NaN -1.26 -8.89 -2.96 -2.28 0.88 -4.76 -12.97 -2.47 1.17 3.56
Vacuole NaN NaN NaN NaN NaN -2.09 -6.45 -1.26 0.94 -0.75 -2.39 -1.33 -1.27 1.26 -0.91
Vacuole Periphery NaN NaN NaN NaN NaN -1.87 -3.76 -1.07 -0.60 -1.02 -2.16 -6.56 -1.07 -0.65 -0.94
Standard name
Human Ortholog
Description Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and in concert with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; interacts with DNA upstream of RNAPII and the non-template strand of the transcription bubble; Spt5p is the only transcription elongation factor conserved in all domains of life
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Spt5

Spt5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available