Standard name
Human Ortholog
Description Delta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.12 0.14 0.11 0.11 0.09
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06 0.08 0.06 0.07
Golgi 0.2 0.14 0.08 0.24 0.32 0 0 0.07 0 0.05 0 0 0.07 0.06 0.05 0 0 0.05 0.43 0.34 0.28 0.37 0.4 0.4
Mitochondria 0.6 0.65 0.69 0.59 0.59 0.89 0.87 0.81 0.93 0.87 0.93 0.91 0.5 0.51 0.5 0.09 0.05 0.07 0 0.09 0.06 0 0.06 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.52 0.5 0.43 0.39 0.24 0.3 0.36 0.36 0.37 0.35 0.37 0.39 0.73 0.78 0.78 0.88 0.89 0.84 0.1 0.13 0.07 0.1 0.07 0.06
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.14 0.31 0.19 0.19 0.26
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 2 12 16 32 22 13 7 2 10 3 3 5 1 2 1 1 0 34 27 70 36 32 31
Bud 1 0 4 6 11 6 4 5 5 3 1 1 2 1 0 0 0 0 0 2 2 2 3 1
Bud Neck 0 0 5 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 3 2 9 2 1 0
Bud Site 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 3 1 3 3 6 7 17 2 12 5 2 3 1 3 10 4 13 0 0 0 0 0 0
Endoplasmic Reticulum 3 1 2 3 19 6 1 1 1 1 1 1 0 0 0 1 1 5 8 3 0 3 1 1
Endosome 0 0 2 4 3 0 2 0 1 0 1 0 1 0 1 2 2 1 18 11 28 25 17 22
Golgi 40 24 32 76 127 23 27 58 4 48 7 10 25 15 18 5 5 10 98 78 140 124 124 137
Mitochondria 121 115 272 186 230 529 680 665 575 920 799 952 172 123 196 19 9 14 9 20 28 13 17 13
Nucleus 0 0 0 0 1 0 3 0 1 3 4 4 2 1 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 2 0 2 6 5 2 3 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 104 88 168 124 92 181 285 295 226 369 321 410 249 187 306 190 172 173 22 30 33 32 22 21
Peroxisomes 0 3 22 10 3 0 5 2 2 2 1 0 5 1 3 4 3 1 23 33 154 64 59 90
SpindlePole 0 0 5 3 0 0 0 0 0 1 1 0 6 1 6 3 5 2 5 6 25 12 16 10
Vac/Vac Membrane 1 3 3 1 14 4 8 26 13 42 20 21 2 6 5 2 3 6 2 6 3 11 4 6
Unique Cell Count 201 177 393 314 391 596 783 821 616 1063 858 1041 341 240 390 217 193 207 231 230 502 333 308 341
Labelled Cell Count 277 240 531 433 535 779 1035 1080 838 1416 1166 1407 472 337 540 237 206 227 231 230 502 333 308 341


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 50.1 46.6 31.7 34.7 34.3 33.2 36.5 39.2 38.0 41.5 37.9 40.4 48.0 50.7 50.3 49.4 49.5 54.5 44.3 44.1 47.8
Std Deviation (1e-4) 12.3 14.3 8.5 8.7 8.3 8.1 9.1 12.7 10.3 12.8 11.9 12.4 14.0 16.1 15.2 21.5 19.9 24.1 13.1 14.3 15.7
Intensity Change (Log2) 0.13 0.11 0.07 0.2 0.3 0.26 0.39 0.26 0.35 0.6 0.68 0.66 0.64 0.64 0.78 0.48 0.48 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.4 3.1 0.5 -1.6 -2.9 -3.6 -2.9 0 0 -1.4 0 -2.7 0 0 0
Bud 0 1.8 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 2.4 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 3.8 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 5.9 8.5 -2.9 -3.5 -0.7 -6.3 -2.7 -6.9 -7.2 -0.4 -0.9 -2.0 -2.9 -2.6 -1.5
Mitochondria -2.8 -3.0 7.7 7.3 4.6 10.2 7.6 11.2 10.6 -5.2 -4.5 -5.4 -14.3 -14.7 -14.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.9 -5.7 -4.0 -2.1 -2.3 -1.9 -2.8 -1.8 -1.2 8.3 8.7 10.2 10.8 10.7 9.6
Peroxisomes -1.5 -3.8 -5.8 -5.4 -6.3 -5.4 -7.2 -6.7 -7.7 -3.0 -3.4 -3.8 -2.2 -2.3 -3.1
SpindlePole 0 0 0 0 0 0 0 0 0 0.5 0 0.3 0 0 0
Vacuole 0 2.7 0 0 2.6 1.7 3.1 1.9 1.7 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 43.6966 53.259 50.9574 48.9538 37.5117 50.0288 58.435 61.4317 60.4943 53.064 56.2078 58.5229 54.7839 62.915 55.6739 50.4602 48.9266 56.6768
Actin 0.0016 0.004 0.0047 0.0531 0.0016 0.0035 0.002 0.0055 0.0032 0.0082 0.0018 0.0033 0.0056 0.0007 0.0038 0.0007 0.0117 0.002
Bud 0.003 0.0002 0.0002 0.0002 0 0.0001 0.0001 0 0 0.0002 0 0 0.0001 0.0001 0.0001 0.0002 0.0001 0
Bud Neck 0.0005 0.0012 0.0008 0.0019 0.0002 0.0004 0.0009 0.0004 0.0004 0.0021 0.0001 0.0002 0.0002 0.0006 0.0005 0.0052 0.0005 0.0006
Bud Periphery 0.0011 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003 0 0 0.0001 0.0001 0.0003 0.0003 0.0001 0
Bud Site 0.0094 0.0038 0.0009 0.0021 0.0002 0.0003 0.0007 0.0005 0.0005 0.0014 0.0001 0.0001 0.0004 0.0016 0.0006 0.0014 0.0003 0.0002
Cell Periphery 0.0011 0.0033 0.0012 0.0013 0.0008 0.0027 0.0011 0.001 0.0009 0.0024 0.0005 0.0006 0.001 0.0011 0.0011 0.0007 0.0006 0.0008
Cytoplasm 0.0001 0 0.0007 0 0.0004 0 0.0001 0 0 0.002 0 0 0.0008 0 0.0001 0.0001 0.0001 0.0001
Cytoplasmic Foci 0.0409 0.0488 0.0337 0.0289 0.1018 0.0244 0.0484 0.0415 0.0355 0.032 0.0471 0.0291 0.0293 0.0544 0.0383 0.0279 0.0743 0.0422
Eisosomes 0.0005 0.0012 0.0007 0.0009 0.0004 0.0008 0.0006 0.0004 0.0004 0.0007 0.0005 0.0004 0.0012 0.0007 0.001 0.0006 0.0006 0.0007
Endoplasmic Reticulum 0 0.0001 0.0002 0 0.0001 0 0.0001 0 0 0.0007 0 0 0.0003 0 0.0002 0.0001 0.0001 0
Endosome 0.0018 0.0019 0.0034 0.002 0.0044 0.0022 0.0019 0.0015 0.0013 0.0035 0.0006 0.0008 0.0056 0.0009 0.002 0.0055 0.0023 0.0004
Golgi 0.0051 0.0217 0.0152 0.0191 0.0133 0.0175 0.0059 0.0132 0.0229 0.0086 0.0075 0.0098 0.0073 0.0052 0.0139 0.0081 0.0053 0.0049
Lipid Particles 0.2412 0.3898 0.3805 0.4701 0.3743 0.4439 0.4625 0.5517 0.507 0.6223 0.5913 0.6824 0.3857 0.4184 0.3974 0.5859 0.343 0.5596
Mitochondria 0.2431 0.112 0.2656 0.2223 0.3722 0.2503 0.2101 0.1327 0.2128 0.1373 0.206 0.1515 0.2369 0.2112 0.2999 0.2508 0.3296 0.2249
None 0.0003 0.0001 0.0005 0.0001 0.0008 0.0001 0.0006 0 0 0.0035 0.0002 0 0.0007 0 0.0002 0.0001 0.0003 0.0004
Nuclear Periphery 0.002 0.0019 0.0009 0.0002 0.0009 0.0007 0.0002 0.0001 0.0001 0.0013 0.0001 0.0002 0.002 0.0001 0.0003 0.0001 0.0002 0.0001
Nucleolus 0.3887 0.3321 0.2041 0.1234 0.0428 0.1645 0.1904 0.1796 0.1467 0.0757 0.0587 0.0671 0.2457 0.2523 0.1621 0.0539 0.1236 0.114
Nucleus 0.0128 0.0126 0.0047 0.0032 0.002 0.0059 0.0035 0.0038 0.0029 0.0032 0.0008 0.0011 0.0085 0.0047 0.0035 0.0017 0.0029 0.0022
Peroxisomes 0.0299 0.0241 0.0508 0.0349 0.0539 0.0345 0.0515 0.0434 0.051 0.0538 0.0703 0.0347 0.0389 0.0345 0.0564 0.0419 0.0964 0.0334
Punctate Nuclear 0.0017 0.0005 0.0117 0.0004 0.0127 0.0007 0.0014 0.0003 0.0005 0.0018 0.0005 0.0003 0.0104 0.0005 0.0004 0.0002 0.0013 0.001
Vacuole 0.0024 0.0092 0.0028 0.004 0.0027 0.0052 0.0024 0.0029 0.0018 0.0093 0.0013 0.0012 0.0019 0.0025 0.003 0.0054 0.0009 0.0012
Vacuole Periphery 0.0128 0.0314 0.0165 0.0318 0.0144 0.0423 0.0156 0.0215 0.0121 0.0298 0.0124 0.0172 0.0173 0.0101 0.0151 0.0092 0.006 0.0114

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 286.866 334.7857 303.7016 305.095 304.8744 218.2612 317.7239 323.4162 336.2687 304.9227
Translational Efficiency 2.0062 1.827 1.6416 1.784 1.5674 2.3148 1.7377 1.3256 1.325 1.588

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1378 1632 127 1335 1734 1751 1773 1446 3112 3383 1900 2781

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 876.29 1196.64 1737.25 1121.50 1117.72 1038.04 1445.27 1148.80 1010.81 1114.55 1464.79 1135.69
Standard Deviation 145.21 349.61 267.28 183.80 235.63 185.63 258.78 199.37 233.78 288.24 269.42 192.54
Intensity Change Log 2 0.449509 0.987325 0.355949 -0.106697 0.370780 0.039569 0.164396 0.674497 0.187210

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001337 0.005322 0.016900 0.023440 0.003510 0.003140 0.010734 0.018416 0.002548 0.004193 0.011146 0.020827
Bud Neck 0.005982 0.004816 0.017634 0.006096 0.003289 0.004030 0.004432 0.005313 0.004481 0.004409 0.005315 0.005688
Bud Site 0.043474 0.091772 0.030354 0.114342* 0.074612 0.056526 0.079446 0.110070* 0.060824 0.073529 0.076165 0.112121*
Cell Periphery 0.000397 0.000988 0.000675 0.000591 0.000572 0.000474 0.000869 0.001064 0.000494 0.000722 0.000856 0.000837
Cytoplasm 0.044558 0.027121 0.001923 0.030667 0.025031 0.021416 0.017673 0.025190 0.033678 0.024168 0.016620 0.027819
Cytoplasmic Foci 0.152879 0.066690 0.091754 0.097580 0.082211 0.097967 0.038432 0.087867 0.113503 0.082879 0.041996 0.092529
Eisosomes 0.000098 0.000088 0.000136 0.000123 0.000071 0.000077 0.000095 0.000142 0.000083 0.000082 0.000098 0.000133
Endoplasmic Reticulum 0.003803 0.001913 0.001702 0.002654 0.002470 0.000929 0.001530 0.002574 0.003060 0.001404 0.001541 0.002612
Endosome 0.100879 0.080816 0.088837 0.073101 0.089935 0.107349 0.048772 0.078650 0.094781 0.094549 0.051450 0.075987
Golgi 0.162881* 0.188903* 0.251096* 0.192414* 0.188564* 0.209358* 0.177607* 0.214370* 0.177191* 0.199491* 0.182519* 0.203830*
Lipid Particles 0.003647 0.002303 0.009729 0.001872 0.001018 0.001259 0.000796 0.001372 0.002182 0.001763 0.001393 0.001612
Mitochondria 0.303500* 0.341518* 0.174470* 0.186967* 0.369419* 0.341928* 0.443164* 0.273087* 0.340230* 0.341730* 0.425204* 0.231746*
Mitotic Spindle 0.036497 0.034448 0.029224 0.172867* 0.037903 0.041417 0.058008 0.086194 0.037280 0.038055 0.056084 0.127801*
None 0.000986 0.002567 0.000632 0.000309 0.000555 0.000465 0.000781 0.000490 0.000746 0.001479 0.000771 0.000403
Nuclear Periphery 0.000485 0.000262 0.006414 0.000241 0.000184 0.000171 0.000325 0.000222 0.000318 0.000215 0.000732 0.000231
Nuclear Periphery Foci 0.001322 0.001565 0.008575 0.000926 0.000651 0.000443 0.000631 0.000705 0.000948 0.000984 0.001162 0.000811
Nucleolus 0.001196 0.000475 0.000893 0.000166 0.000106 0.000289 0.000130 0.000143 0.000589 0.000379 0.000181 0.000154
Nucleus 0.003461 0.001908 0.000344 0.000411 0.000310 0.001169 0.000179 0.000338 0.001705 0.001526 0.000190 0.000373
Peroxisomes 0.014770 0.011901 0.011047 0.022689 0.009583 0.013883 0.012128 0.026700 0.011880 0.012927 0.012056 0.024774
Vacuole 0.057425 0.067781 0.142093 0.045775 0.049185 0.049356 0.046492 0.037644 0.052834 0.058245 0.052882 0.041547
Vacuole Periphery 0.060422 0.066841 0.115568* 0.026770 0.060821 0.048353 0.057776 0.029450 0.060644 0.057272 0.061639 0.028163

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.60 -2.28 -10.46 -8.30 -0.89 0.58 -5.72 -7.60 -7.74 -3.39 -3.73 -7.00 -12.68 -11.33 -5.15
Bud Neck 1.40 -3.66 -0.58 -2.50 3.54 -1.63 -2.14 -3.43 -2.13 -1.28 0.17 -1.46 -2.79 -3.26 -0.88
Bud Site -10.16 1.69 -13.61 -3.95 -10.05 4.02 -1.23 -6.07 -9.64 -5.05 -3.84 -4.11 -12.90 -9.50 -7.62
Cell Periphery -5.52 -2.54 -2.10 4.62 1.01 1.68 -4.53 -5.13 -6.45 -1.98 -3.87 -5.91 -5.21 -1.69 0.40
Cytoplasm 4.47 13.53 2.98 -1.84 -13.96 1.43 3.38 -0.44 -1.81 -3.68 4.26 8.20 1.88 -2.57 -6.85
Cytoplasmic Foci 17.48 5.11 10.28 -7.33 -0.43 -4.10 14.09 -1.47 2.31 -13.88 9.81 23.52 6.25 -3.19 -17.38
Eisosomes 0.62 -1.57 -1.31 -5.58 0.80 -1.32 -4.60 -9.19 -8.29 -5.60 0.14 -1.65 -5.47 -9.98 -5.60
Endoplasmic Reticulum 2.57 2.72 1.39 -1.48 -1.74 3.79 2.04 -0.15 -3.37 -1.95 4.18 3.44 0.91 -3.49 -2.69
Endosome 4.66 1.49 6.67 2.39 2.18 -4.29 12.91 3.08 7.32 -9.63 0.09 16.04 6.86 7.08 -9.73
Golgi -3.45 -3.58 -2.98 0.21 2.56 -2.73 1.53 -2.88 -0.38 -4.22 -4.14 -0.74 -3.96 -0.14 -2.62
Lipid Particles 2.41 -2.28 2.92 0.52 2.90 -0.82 0.93 -1.44 -0.51 -2.51 1.30 2.60 1.45 0.11 -1.18
Mitochondria -3.14 5.33 10.79 13.89 -0.32 2.45 -6.01 8.41 6.16 14.06* -0.14 -8.11 13.62 13.84 19.19*
Mitotic Spindle 0.58 0.93 -17.99 -18.60 -14.78 -1.06 -5.62 -9.20 -8.44 -5.09 -0.33 -5.98 -19.60 -19.55 -14.15
None -2.65 2.47 5.25 3.87 4.63 1.76 -6.50 0.71 -0.14 3.07 -2.55 -0.35 4.53 3.77 6.90
Nuclear Periphery 1.92 -2.89 2.17 0.17 3.01 0.29 -3.19 -0.72 -0.93 1.69 1.78 -2.73 1.39 -0.58 3.36
Nuclear Periphery Foci -0.42 -1.70 0.63 1.20 1.78 0.73 0.18 -0.43 -1.07 -0.63 -0.27 -0.57 0.36 0.67 0.94
Nucleolus 2.62 0.88 3.89 3.01 3.16 -2.09 -1.03 -1.17 1.35 -0.73 1.44 3.48 3.46 2.95 0.15
Nucleus 2.46 6.86 6.24 2.99 -2.26 -1.99 2.57 -0.94 1.79 -2.52 0.36 7.39 5.74 3.32 -4.59
Peroxisomes 1.54 1.38 -3.27 -4.86 -3.95 -3.00 -1.76 -7.48 -5.34 -6.07 -0.92 -0.12 -7.86 -7.21 -7.11
Vacuole -2.77 -5.75 1.68 4.33 6.18 -0.04 0.72 3.46 3.51 2.78 -2.30 -0.22 3.40 5.57 3.12
Vacuole Periphery -1.59 -4.50 10.11 11.96 7.42 3.76 0.86 9.81 6.93 8.94 1.30 -0.28 14.09 13.53 11.75