Standard name
Human Ortholog
Description Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0.08 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.98 1.0 1.0 0.99 0.98 0.98 0.98 0.98 0.98 0.97 – 0.96 0.98 0.97 0.94 0.96 0.92 0.84 0.93 0.84 0.81 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0
Bud 0 1 0 0 0 2 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 2 1 2 1 3 0 0 0 0 0 0
Cytoplasm 70 83 99 125 254 313 348 346 179 216 164 183 – 24 107 115 91 113 81 78 87 21 28 59
Endoplasmic Reticulum 1 0 1 0 1 1 6 7 5 4 2 2 – 1 1 4 6 5 0 0 1 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 1 2 1 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 1 0 1 2 1 0 0 0 0 0 0
Mitochondria 0 1 0 0 0 0 0 0 1 0 0 0 – 0 0 2 3 4 0 0 0 0 0 0
Nucleus 1 1 0 0 0 1 0 0 0 1 0 1 – 0 1 0 1 1 2 2 0 0 1 0
Nuclear Periphery 0 0 1 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 1 0 0 0 2 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 1 1 1 0 0 1 1 0 2 0 2 – 0 0 1 0 1 2 4 0 1 0 1
Unique Cell Count 71 86 101 125 255 316 355 352 183 220 167 188 25 109 119 97 118 89 94 94 26 35 69
Labelled Cell Count 72 88 102 126 256 319 355 354 185 223 167 188 29 110 126 107 129 89 94 94 26 35 69


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.0 17.6 14.9 15.4 16.2 16.8 16.0 17.0 17.1 16.6 18.1 17.2 – 17.8 18.8 19.2 18.6 20.2 – – 20.8
Std Deviation (1e-4) 2.8 4.9 3.0 2.4 2.6 3.1 3.3 3.3 3.6 3.6 4.1 3.6 – 3.0 3.0 5.7 5.4 6.2 – – 2.8
Intensity Change (Log2) – – – 0.05 0.12 0.17 0.1 0.19 0.2 0.16 0.28 0.21 – 0.26 0.33 0.36 0.32 0.44 – – 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 1.6 1.5 0.8 0 0.2 -0.1 0.1 0.1 -0.4 – -0.6 0.1 -0.6 -1.5 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.1102 11.5393 9.9824 11.8268 10.2275 10.7117 11.778 15.7616 12.0305 11.7806 13.7999 13.1523 6.9331 12.8811 9.4255 6.5115 11.2211 12.0738
Actin 0.0867 0.0005 0.0894 0.0002 0.0001 0.0002 0.084 0.0006 0.0018 0.001 0.0001 0.0002 0.3078 0.0002 0.1051 0 0.0003 0.0001
Bud 0.0018 0.0002 0.0016 0.0001 0.0001 0.0002 0.0021 0.0001 0.0002 0.0001 0.0001 0 0.0003 0.0001 0.0003 0.0001 0.0001 0
Bud Neck 0.0029 0.0002 0.0028 0.0005 0.0003 0.0006 0.0002 0.0002 0.0002 0.0004 0.0006 0.0008 0.0022 0.0001 0.0009 0.0001 0.0005 0.0003
Bud Periphery 0.0027 0 0.0013 0 0 0 0.0024 0 0.0004 0 0 0 0.0003 0 0.0001 0 0 0
Bud Site 0.0095 0.001 0.0125 0 0 0.0001 0.0029 0.0002 0.0003 0.0001 0 0 0.0007 0.0001 0.0016 0 0 0
Cell Periphery 0.0007 0 0.0003 0 0 0 0.0001 0 0.0005 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.6545 0.9529 0.7415 0.9671 0.646 0.9313 0.7092 0.9888 0.8156 0.8982 0.9874 0.9677 0.6606 0.9896 0.8196 0.6372 0.9866 0.9924
Cytoplasmic Foci 0.039 0.0043 0.0328 0.0015 0.0003 0.003 0.0628 0.0004 0.0022 0.0034 0.0006 0.002 0.0033 0.0003 0.0241 0.0181 0.0016 0.0006
Eisosomes 0.0006 0 0.0003 0 0 0 0.0003 0 0 0 0 0 0.001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0305 0.0013 0.0084 0.0004 0.0004 0.0013 0.0036 0.0003 0.0683 0.0016 0.0004 0.001 0.0019 0.0002 0.0007 0.0068 0.0006 0.0004
Endosome 0.0442 0.0034 0.0411 0.0004 0.0002 0.0273 0.0272 0.0001 0.0197 0.0008 0.0001 0.0006 0.0032 0.0001 0.0014 0.2964 0.0002 0.0001
Golgi 0.0136 0.0002 0.0297 0 0 0.0006 0.0094 0 0.0029 0.0001 0 0 0.0067 0 0.0027 0.0158 0 0
Lipid Particles 0.0131 0.0002 0.0046 0 0 0 0.0256 0 0.0009 0 0 0 0.0018 0 0.0006 0.0001 0 0
Mitochondria 0.0157 0.0001 0.0037 0.0001 0.0001 0.0004 0.0059 0.0001 0.0027 0.0001 0 0.0001 0.0004 0.0001 0.001 0.0007 0 0
None 0.0099 0.0191 0.0011 0.0012 0.0004 0.0008 0.0056 0.0004 0.0631 0.0022 0.0004 0.0046 0.0023 0.0004 0.001 0.0009 0.0014 0.0005
Nuclear Periphery 0.022 0.0022 0.0051 0.0024 0.0084 0.0028 0.0132 0.0008 0.0082 0.0025 0.0009 0.0021 0.0012 0.0011 0.0011 0.0081 0.0008 0.0005
Nucleolus 0.0009 0.0001 0.0002 0 0.0001 0 0.0003 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.0193 0.0104 0.0105 0.0244 0.339 0.0206 0.0086 0.0078 0.0042 0.0884 0.0088 0.0196 0.0037 0.0074 0.0116 0.0059 0.0064 0.0048
Peroxisomes 0.0114 0.0001 0.0087 0 0 0 0.0268 0 0 0 0 0 0.0012 0 0.0262 0.0001 0 0
Punctate Nuclear 0.0099 0.0017 0.0025 0.0014 0.0045 0.0007 0.0083 0.0002 0.0003 0.0009 0.0002 0.0009 0.0012 0.0001 0.0018 0.0003 0.0014 0.0001
Vacuole 0.0069 0.0018 0.0013 0.0002 0.0001 0.0071 0.0007 0.0001 0.0067 0.0001 0.0003 0.0002 0.0001 0.0002 0.0001 0.0061 0.0001 0.0001
Vacuole Periphery 0.0041 0.0002 0.0005 0.0001 0 0.0028 0.0008 0 0.0016 0 0.0001 0.0001 0.0001 0 0 0.0032 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 96.7899 94.3901 53.6985 98.4987 96.9713 100.254 95.4377 107.3567 105.6341 111.8049
Translational Efficiency 2.335 1.9719 2.6588 1.7655 2.0308 2.4405 2.0151 1.5326 1.841 1.8306

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
172 1215 2667 1879 2070 895 1625 10 2242 2110 4292 1889

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1305.99 1498.53 1632.59 1916.90 1418.08 1432.98 1627.49 413.47 1409.48 1470.73 1630.66 1908.94
Standard Deviation 260.93 319.25 275.98 372.70 223.63 250.51 290.78 5.32 228.67 293.87 281.69 387.39
Intensity Change Log 2 — 0.198404 0.322019 0.553631 — 0.015080 0.198710 -1.778084 — 0.105880 0.259144 -0.225205

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001319 0.001735 0.000442 0.001048 0.000167 0.000886 0.000214 0.000274 0.000255 0.001375 0.000356 0.001044
Bud Neck 0.004563 0.000724 0.001025 0.002564 0.000451 0.000696 0.001088 0.000007 0.000767 0.000712 0.001049 0.002551
Bud Site 0.023266 0.004619 0.002567 0.006130 0.001804 0.006548 0.003872 0.000018 0.003450 0.005437 0.003061 0.006098
Cell Periphery 0.000475 0.000088 0.000049 0.000150 0.000051 0.000093 0.000083 0.000197 0.000084 0.000091 0.000062 0.000151
Cytoplasm 0.759590* 0.829165* 0.732355* 0.641259* 0.850777* 0.845635* 0.784215* 0.000024 0.843781* 0.836151* 0.751990* 0.637865*
Cytoplasmic Foci 0.091801 0.014035 0.002638 0.009931 0.016562 0.029368 0.007267 0.000021 0.022334 0.020538 0.004391 0.009878
Eisosomes 0.000025 0.000005 0.000002 0.000010 0.000003 0.000008 0.000002 0.001281 0.000005 0.000007 0.000002 0.000017
Endoplasmic Reticulum 0.003598 0.000463 0.000097 0.000613 0.001156 0.001076 0.000075 0.000000 0.001343 0.000723 0.000089 0.000609
Endosome 0.004317 0.001108 0.000277 0.009623 0.000775 0.003240 0.001781 0.000000 0.001046 0.002012 0.000847 0.009572
Golgi 0.002991 0.001621 0.000643 0.005593 0.000765 0.003229 0.001748 0.000004 0.000936 0.002303 0.001062 0.005564
Lipid Particles 0.004041 0.000721 0.000231 0.003325 0.000738 0.001601 0.000604 0.000029 0.000992 0.001094 0.000372 0.003308
Mitochondria 0.000060 0.000160 0.000332 0.000458 0.000097 0.001849 0.000169 0.000008 0.000094 0.000876 0.000270 0.000456
Mitotic Spindle 0.005558 0.005346 0.010964 0.038509 0.002055 0.013614 0.019135 0.000267 0.002324 0.008853 0.014058 0.038306
None 0.000602 0.009342 0.009347 0.009791 0.003240 0.004250 0.008348 0.997554* 0.003037 0.007182 0.008969 0.015020
Nuclear Periphery 0.000198 0.000165 0.000196 0.000416 0.000147 0.000117 0.000148 0.000000 0.000151 0.000145 0.000178 0.000414
Nuclear Periphery Foci 0.011117 0.004654 0.000465 0.001771 0.003408 0.005298 0.001200 0.000002 0.004000 0.004927 0.000743 0.001761
Nucleolus 0.000116 0.000185 0.000154 0.000338 0.000130 0.000410 0.000136 0.000002 0.000128 0.000280 0.000147 0.000336
Nucleus 0.035173 0.108339 0.227781* 0.232274* 0.102578 0.060470 0.147060* 0.000007 0.097407 0.088035 0.197219* 0.231044*
Peroxisomes 0.000281 0.000338 0.000302 0.001306 0.000050 0.000995 0.000528 0.000304 0.000068 0.000617 0.000388 0.001301
Vacuole 0.050390 0.016829 0.010013 0.030122 0.014800 0.019775 0.021941 0.000000 0.017530 0.018079 0.014529 0.029963
Vacuole Periphery 0.000519 0.000357 0.000120 0.004769 0.000248 0.000843 0.000384 0.000000 0.000269 0.000563 0.000220 0.004744

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.16 3.86 2.21 1.34 -2.05 NaN NaN NaN NaN NaN -2.55 0.24 -2.11 1.34 -2.16
Bud Neck 1.77 1.49 1.27 -5.01 -1.89 NaN NaN NaN NaN NaN -0.98 -3.90 -5.27 -5.70 -2.50
Bud Site 3.20 3.74 3.35 0.88 -4.34 NaN NaN NaN NaN NaN -2.80 0.67 -1.45 1.68 -2.86
Cell Periphery 4.91 5.48 4.33 -2.99 -6.79 NaN NaN NaN NaN NaN -2.00 -0.41 -4.82 -3.75 -5.15
Cytoplasm -3.64 3.82 6.97 28.12 9.12 NaN NaN NaN NaN NaN 3.65 33.18 36.58 32.12 12.53
Cytoplasmic Foci 8.77 10.40 9.95 8.14 -6.80 NaN NaN NaN NaN NaN 0.47 14.49 12.65 12.44 -2.23
Eisosomes 3.59 4.17 3.49 -0.47 -4.13 NaN NaN NaN NaN NaN -1.90 2.46 -2.81 -2.23 -3.33
Endoplasmic Reticulum 2.79 3.10 2.93 1.31 -2.03 NaN NaN NaN NaN NaN 2.05 3.94 3.20 2.26 -2.07
Endosome 5.19 6.86 2.56 -1.86 -3.25 NaN NaN NaN NaN NaN -2.11 4.23 -1.88 -0.65 -2.97
Golgi 1.35 2.52 1.44 0.01 -2.12 NaN NaN NaN NaN NaN -1.95 2.18 -0.78 0.98 -2.01
Lipid Particles 3.55 4.11 2.48 -2.25 -3.74 NaN NaN NaN NaN NaN -1.22 1.43 -2.34 -1.72 -3.09
Mitochondria -1.67 -5.36 -8.66 -2.49 0.77 NaN NaN NaN NaN NaN -2.14 -4.97 -7.51 1.49 -0.72
Mitotic Spindle -0.19 -2.07 -2.72 -3.92 -1.27 NaN NaN NaN NaN NaN -3.92 -8.13 -6.28 -2.93 -1.59
None -7.75 -34.13* -26.88* -0.69 0.69 NaN NaN NaN NaN NaN -5.59 -22.42 -6.49 -4.31 -3.52
Nuclear Periphery 0.09 -3.32 -3.70 -7.02 -1.28 NaN NaN NaN NaN NaN -0.65 -15.02 -12.85 -10.18 -2.60
Nuclear Periphery Foci 4.07 6.85 6.43 9.83 -5.53 NaN NaN NaN NaN NaN -2.17 6.51 5.84 11.42 -1.91
Nucleolus -1.24 -2.14 -2.57 -1.06 -0.10 NaN NaN NaN NaN NaN -2.18 -2.23 -3.33 0.33 -1.05
Nucleus -12.76 -34.42 -31.15 -26.02 1.79 NaN NaN NaN NaN NaN 1.72 -33.85 -30.62 -32.46 -5.29
Peroxisomes 0.01 2.09 -0.31 -0.28 -1.30 NaN NaN NaN NaN NaN -4.43 -1.34 -1.80 0.88 -1.24
Vacuole 4.54 3.48 -2.89 -15.32 -13.50 NaN NaN NaN NaN NaN -4.51 -18.13 -18.79 -16.28 -8.01
Vacuole Periphery 1.08 3.42 -1.09 -1.48 -1.97 NaN NaN NaN NaN NaN -1.31 2.12 -1.58 -1.16 -1.94
Standard name
Human Ortholog
Description Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ypt7

Ypt7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypt7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available