Standard name
Human Ortholog
Description Alpha-1,2-mannosyltransferase; responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.1 – 0.14 0.12 0.11 0.11 – 0.1 0.1 0.08 0.07 – 0.05 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0.07 – 0.09 0.09 0.09 0.06 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.34 – 0.05 0.09 0.11 0.07 – 0 0.08 0.06 0.07 – 0.08 0.41 0.1 0.14 0.1 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0.08 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.15 0.09 – 0.42 0.38 0.28 0.39 – 0.21 0.25 0.11 0.25 – 0.27 0.34 0.09 0.09 0.12 0.32 0.25 0.25 0.11 0.19 0.26
Golgi 0.08 0.19 – 0.34 0.3 0.22 0.23 – 0.17 0.25 0.07 0.11 – 0.35 0 0.1 0.19 0.19 0.29 0.37 0.1 0.3 0.4 0.36
Mitochondria 0.5 0.09 – 0.16 0.05 0.27 0.23 – 0.56 0.32 0.74 0.45 – 0.13 0 0.05 0 0.07 0.06 0.05 0.05 0.17 0.09 0.08
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 – 0 0 0.05 0 – 0.07 0 0.06 0.06 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0.1 0.1 0.07 0.12 – 0.1 0.14 0 0.08 – 0.06 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.43 0.36 – 0.17 0.18 0.11 0.08 – 0.09 0.09 0.05 0.1 – 0.32 0.39 0.82 0.77 0.75 0.28 0.28 0.49 0.29 0.27 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 10 – 22 20 20 37 – 19 26 14 15 – 3 1 7 4 7 0 1 0 0 0 0
Bud 1 0 – 3 3 6 24 – 17 23 16 12 – 0 2 0 1 2 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 2 – 1 0 4 2 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 – 2 0 1 3 – 1 0 0 2 – 0 0 1 0 1 0 0 0 0 0 0
Cytoplasm 13 33 – 8 15 20 22 – 7 21 10 15 – 5 51 28 41 23 1 2 0 1 0 2
Endoplasmic Reticulum 5 8 – 2 7 7 3 – 3 2 3 6 – 2 1 10 8 12 1 0 0 0 0 0
Endosome 19 9 – 66 62 51 126 – 42 67 19 55 – 17 42 25 27 27 47 31 12 2 16 20
Golgi 11 19 – 53 50 40 75 – 33 67 13 23 – 22 3 28 54 42 43 47 5 7 34 28
Mitochondria 65 9 – 25 9 50 74 – 110 86 131 97 – 8 3 15 13 15 9 6 2 4 7 6
Nucleus 1 0 – 2 0 0 2 – 1 1 0 1 – 2 1 9 3 3 0 0 0 1 1 2
Nuclear Periphery 0 0 – 0 0 0 1 – 0 0 0 0 – 0 0 0 0 1 1 0 0 0 0 0
Nucleolus 7 0 – 2 1 9 9 – 14 8 10 12 – 2 0 1 2 2 0 0 0 0 0 0
Peroxisomes 0 0 – 1 7 4 11 – 1 11 2 4 – 0 1 0 0 0 2 1 1 0 0 0
SpindlePole 1 2 – 16 16 13 40 – 19 37 7 18 – 4 4 1 1 3 0 0 0 0 0 0
Vac/Vac Membrane 56 35 – 27 30 20 26 – 18 24 9 22 – 20 48 233 222 168 41 36 24 7 23 14
Unique Cell Count 130 98 157 164 184 322 197 267 176 217 63 124 283 289 223 148 129 51 25 85 80
Labelled Cell Count 179 126 229 220 241 455 286 373 238 284 85 157 358 376 306 148 129 51 25 85 80


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 8.5 – 8.9 10.2 8.6 8.8 – 7.3 7.9 6.7 7.3 – 9.0 7.8 12.4 12.4 12.5 – 8.3 8.7
Std Deviation (1e-4) 1.1 1.3 – 1.3 1.7 1.3 1.4 – 1.0 1.1 1.1 1.1 – 1.3 1.6 2.7 2.7 2.8 – 1.7 1.2
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7153 6.0023 5.2128 5.3189 4.3975 5.2804 4.2467 6.3393 6.0397 5.9792 4.5018 5.9706 3.2739 5.1398 4.8013 4.0406 3.9514 4.4959
Actin 0.0082 0.0086 0.0096 0.0052 0.011 0.0062 0.0161 0.0017 0.008 0.0001 0.0003 0.0102 0.0385 0.005 0.011 0.0013 0.0431 0.0023
Bud 0.0005 0.0032 0.0002 0.0003 0.0004 0.0001 0.0004 0.0001 0.0003 0 0.0002 0.0001 0.0003 0.0086 0.0001 0.0001 0.0002 0
Bud Neck 0.0003 0.0002 0.0008 0.0004 0.0008 0.0003 0.0006 0.0001 0.0015 0.0001 0.0002 0.0003 0.0005 0.0002 0.0002 0.0002 0.0005 0.0002
Bud Periphery 0.0002 0.0014 0.0003 0.0006 0.0003 0.0001 0.0005 0.0001 0.0004 0 0.0002 0.0002 0.0004 0.0033 0.0001 0.0001 0.0005 0
Bud Site 0.0006 0.0017 0.0006 0.0005 0.0007 0.0001 0.0029 0.0002 0.0012 0 0.0046 0.0002 0.0011 0.0077 0.0004 0.0003 0.001 0.0001
Cell Periphery 0.0003 0.0002 0.0002 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0002 0 0 0.0002 0
Cytoplasm 0.0037 0.0026 0.0015 0.0008 0.0095 0.001 0.008 0.0129 0.0024 0.0003 0.0011 0.0009 0.0038 0.0035 0.0028 0.0022 0.0015 0.0014
Cytoplasmic Foci 0.0812 0.1032 0.0753 0.0574 0.0972 0.0593 0.0786 0.0903 0.0646 0.0349 0.0597 0.0439 0.0798 0.0824 0.0826 0.0786 0.0654 0.0543
Eisosomes 0.0002 0 0.0001 0.0001 0 0 0.0003 0 0.0001 0 0 0.0002 0.0004 0 0.0001 0 0.0016 0
Endoplasmic Reticulum 0.0011 0.0007 0.0012 0.0007 0.002 0.0011 0.0025 0.0015 0.0011 0.0004 0.001 0.001 0.0024 0.0003 0.001 0.0024 0.0024 0.0005
Endosome 0.3145 0.3382 0.3038 0.3031 0.3007 0.268 0.3189 0.3774 0.3134 0.3682 0.3353 0.3524 0.2931 0.2604 0.2443 0.3057 0.3691 0.2913
Golgi 0.4944 0.4815 0.5323 0.5643 0.4739 0.5885 0.4393 0.4549 0.5381 0.5512 0.4844 0.4976 0.4794 0.6018 0.6133 0.4882 0.4637 0.606
Lipid Particles 0.0264 0.0177 0.0179 0.0168 0.0247 0.0151 0.0388 0.0138 0.018 0.0107 0.0453 0.0275 0.0255 0.0081 0.0055 0.0198 0.015 0.024
Mitochondria 0.0227 0.0133 0.0241 0.0233 0.0127 0.0264 0.027 0.0096 0.0177 0.0136 0.0229 0.0268 0.0294 0.0088 0.01 0.0041 0.0079 0.0072
None 0.0081 0.0008 0.0008 0.0016 0.002 0.0001 0.0075 0.0004 0.0006 0 0.0003 0.0002 0.0033 0.0002 0.0036 0.0016 0.0016 0
Nuclear Periphery 0.0042 0.0004 0.0013 0.0006 0.0128 0.0033 0.0111 0.0007 0.0015 0.0002 0.001 0.0024 0.0048 0.0001 0.0011 0.0042 0.0046 0.0002
Nucleolus 0.0024 0.0016 0.0003 0.0002 0.0008 0.0021 0.0013 0 0.002 0 0.0005 0.0001 0.0062 0.0001 0.0001 0.0002 0.0002 0
Nucleus 0.0021 0.0004 0.0004 0.0002 0.0099 0.0041 0.0082 0.0002 0.0012 0 0.0002 0.0001 0.0015 0.0001 0.0002 0.0005 0.0003 0
Peroxisomes 0.0114 0.011 0.014 0.0058 0.0188 0.0048 0.0175 0.0164 0.0149 0.0008 0.0076 0.0041 0.0194 0.0041 0.0116 0.0383 0.0095 0.0035
Punctate Nuclear 0.0032 0.0002 0.0026 0.0005 0.0066 0.0009 0.0048 0.0001 0.0017 0 0.0007 0.0002 0.0023 0.0001 0.0028 0.0033 0.0021 0.0001
Vacuole 0.0074 0.006 0.0059 0.0096 0.0054 0.0076 0.0063 0.0109 0.0052 0.0076 0.0154 0.0114 0.0037 0.0023 0.0034 0.0179 0.0033 0.0033
Vacuole Periphery 0.007 0.0072 0.0069 0.0078 0.0097 0.0109 0.0091 0.0086 0.0061 0.0116 0.019 0.02 0.0042 0.0026 0.0054 0.0309 0.0064 0.0056

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.9837 57.6926 39.4853 42.8386 58.4918 54.4821 48.2804 41.4732 38.8079 58.3627
Translational Efficiency 1.347 1.2222 1.2471 1.1307 1.1606 1.1309 1.1059 1.1275 1.0031 1.0204

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
101 1509 822 2071 2161 1770 2026 1120 2262 3279 2848 3191

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 899.19 860.18 1154.79 971.75 866.14 878.03 1078.47 956.77 867.62 869.82 1100.50 966.49
Standard Deviation 140.96 139.89 153.88 129.99 116.26 138.19 131.70 127.96 117.67 139.26 142.72 129.48
Intensity Change Log 2 — -0.063987 0.360933 0.111959 — 0.019670 0.316314 0.143572 — -0.022336 0.339213 0.127556

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.059435 0.002406 0.013812 0.010404 0.000611 0.002035 0.003819 0.006192 0.003238 0.002206 0.006703 0.008926
Bud Neck 0.003169 0.000380 0.000970 0.004064 0.000176 0.000134 0.000452 0.004260 0.000310 0.000247 0.000601 0.004133
Bud Site 0.022405 0.003126 0.012336 0.024937 0.001467 0.000997 0.002503 0.021310 0.002402 0.001977 0.005341 0.023664
Cell Periphery 0.000646 0.000083 0.000169 0.000102 0.000025 0.000047 0.000022 0.000059 0.000053 0.000064 0.000064 0.000087
Cytoplasm 0.011028 0.001180 0.014707 0.048968 0.002052 0.000806 0.002054 0.037790 0.002453 0.000978 0.005706 0.045045
Cytoplasmic Foci 0.235223* 0.184161* 0.314923* 0.226656* 0.215978* 0.206527* 0.310300* 0.239147* 0.216837* 0.196234* 0.311635* 0.231040*
Eisosomes 0.001089 0.000042 0.000055 0.000048 0.000020 0.000029 0.000019 0.000031 0.000068 0.000035 0.000030 0.000042
Endoplasmic Reticulum 0.008834 0.000409 0.027269 0.001103 0.000135 0.000212 0.000275 0.000424 0.000524 0.000303 0.008066 0.000865
Endosome 0.200691* 0.308501* 0.145543 0.287769* 0.241725* 0.287940* 0.231722* 0.324137* 0.239893* 0.297402* 0.206849* 0.300534*
Golgi 0.358944* 0.435458* 0.351170* 0.173363* 0.482172* 0.462589* 0.398543* 0.119517* 0.476670* 0.450103* 0.384870* 0.154464*
Lipid Particles 0.023562 0.008528 0.026026 0.005233 0.002038 0.007026 0.003546 0.004228 0.002999 0.007717 0.010035 0.004880
Mitochondria 0.010077 0.010665 0.000309 0.006200 0.011539 0.009182 0.001830 0.004035 0.011474 0.009865 0.001391 0.005440
Mitotic Spindle 0.016293 0.000586 0.036105 0.025291 0.001904 0.000673 0.011691 0.037869 0.002546 0.000633 0.018737 0.029706
None 0.000092 0.000028 0.000028 0.000044 0.000026 0.000015 0.000006 0.000463 0.000029 0.000021 0.000012 0.000191
Nuclear Periphery 0.000234 0.000022 0.000440 0.000205 0.000029 0.000025 0.000136 0.000311 0.000038 0.000023 0.000224 0.000242
Nuclear Periphery Foci 0.001651 0.000207 0.017010 0.000716 0.000115 0.000162 0.001061 0.001680 0.000184 0.000183 0.005664 0.001054
Nucleolus 0.001272 0.000051 0.000017 0.000161 0.000044 0.000026 0.000010 0.000505 0.000099 0.000037 0.000012 0.000282
Nucleus 0.002131 0.000053 0.000206 0.001345 0.000060 0.000030 0.000017 0.001339 0.000152 0.000040 0.000071 0.001343
Peroxisomes 0.025124 0.006962 0.002808 0.026682 0.005261 0.007494 0.003716 0.021038 0.006148 0.007249 0.003454 0.024701
Vacuole 0.011290 0.024121 0.032762 0.148136 0.021814 0.008223 0.022810 0.166539 0.021344 0.015539 0.025683 0.154595
Vacuole Periphery 0.006809 0.013035 0.003332 0.008573 0.012808 0.005827 0.005468 0.009126 0.012540 0.009144 0.004852 0.008767

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.46 2.74 3.02 -5.37 1.44 -3.13 -3.93 -4.05 -2.73 -1.17 1.22 -2.85 -4.10 -6.12 -1.16
Bud Neck 2.14 1.62 -1.44 -13.66 -11.44 0.86 -1.19 -8.62 -8.68 -7.40 0.86 -1.72 -15.70 -16.13 -12.69
Bud Site 3.26 1.59 -0.72 -14.34 -6.61 1.52 -3.43 -10.11 -10.32 -9.47 0.95 -5.51 -17.80 -18.28 -14.80
Cell Periphery 3.64 2.86 3.52 -0.79 1.16 -2.24 0.99 -3.69 -0.76 -4.24 -1.02 -0.63 -2.13 -1.48 -0.88
Cytoplasm 2.87 -1.35 -11.91 -20.60 -13.32 2.24 -0.70 -13.33 -13.56 -13.23 3.70 -5.59 -24.02 -24.62 -21.84
Cytoplasmic Foci 3.15 -4.56 1.42 -5.15 13.60 1.91 -17.69 -0.78 -2.18 12.87 4.90 -19.76 0.36 -4.60 20.41
Eisosomes 3.29 3.25 3.28 -0.96 0.87 -3.65 0.17 -3.59 -0.59 -3.76 2.19 2.49 1.75 -1.94 -2.98
Endoplasmic Reticulum 2.67 -3.73 2.40 -2.30 6.62 -1.19 -2.71 -7.70 -3.54 -2.96 1.42 -6.48 -1.61 -2.88 6.07
Endosome -6.52 3.44 -3.68 6.60 -18.89 -7.47 1.88 -6.26 -0.61 -7.71 -10.81 6.75 -5.71 4.74 -13.21
Golgi -3.33 0.51 9.05 36.82* 19.26* 2.62 11.58 51.42* 47.61* 39.26* 4.09 13.86 54.97* 58.13* 42.36*
Lipid Particles 2.41 -0.45 2.92 1.85 9.52 -4.60 -2.97 -5.06 2.00 -1.75 -4.93 -8.77 -4.88 2.50 6.18
Mitochondria -0.15 2.73 1.18 3.58 -8.68 1.48 8.79 5.83 3.84 -2.17 1.20 9.57 5.57 4.90 -6.87
Mitotic Spindle 1.69 -2.03 -1.42 -12.48 1.51 3.34 -7.76 -9.67 -9.99 -6.78 3.60 -10.77 -14.56 -15.89 -5.66
None 1.29 1.26 0.51 -2.74 -2.68 1.26 3.19 -1.52 -1.55 -1.58 0.98 2.38 -1.74 -1.81 -1.89
Nuclear Periphery 1.67 -1.48 -0.70 -11.67 1.59 0.32 -4.35 -8.34 -8.49 -5.65 1.25 -6.45 -12.44 -14.07 -3.77
Nuclear Periphery Foci 1.85 -6.55 0.66 -4.69 7.12 -0.78 -4.11 -4.88 -4.75 -1.77 0.05 -8.11 -6.52 -6.70 6.18
Nucleolus 1.09 1.12 0.92 -6.66 -11.16 0.58 1.06 -2.54 -2.64 -2.69 1.07 1.49 -2.77 -3.84 -4.11
Nucleus 1.44 1.30 -0.40 -8.56 -7.83 0.84 1.15 -6.02 -6.11 -6.13 1.55 0.89 -9.70 -10.52 -10.33
Peroxisomes 2.63 3.24 -0.02 -9.71 -12.46 -1.97 1.72 -7.62 -6.16 -8.58 -1.18 3.25 -11.66 -11.32 -14.67
Vacuole -2.95 -5.06 -26.01 -26.76 -23.91 7.37 -1.10 -22.57 -24.51 -22.23 3.03 -2.90 -35.48 -37.41 -34.37
Vacuole Periphery -2.95 1.99 -0.53 3.97 -7.36 6.29 7.40 3.50 -2.05 -2.62 3.13 8.39 4.66 1.49 -5.35
Standard name
Human Ortholog
Description Alpha-1,2-mannosyltransferase; responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
Localization
Cell Percentages Golgi (53%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mnn5

Mnn5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mnn5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available