Standard name
Human Ortholog
Description Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0.06 0 0.05 0 0 0 0 0 0
Bud 0 0 – 0 0.05 0 0 0 0.05 0 0.08 0.05 – 0 0 0.07 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0.07 0 0.05 0 0 0 0 0 0
Cytoplasm 0.35 0.42 – 0.61 0.45 0.23 0.27 0.19 0.13 0.16 0.09 0.12 – 0.8 0.94 0.2 0.2 0.32 0.06 0.07 0.05 0.11 0.11 0.16
Endoplasmic Reticulum 0 0.05 – 0.09 0 0 0 0 0 0 0 0 – 0.05 0 0.06 0.05 0.07 0 0 0 0.06 0 0
Endosome 0 0.11 – 0 0.15 0.07 0.1 0.07 0 0 0 0 – 0 0 0.16 0.24 0.12 0.14 0.11 0 0 0.09 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.64 0.37 – 0.17 0.15 0.66 0.47 0.49 0.79 0.71 0.89 0.78 – 0 0 0.14 0.14 0.15 0.63 0.59 0.63 0.65 0.55 0.57
Nucleus 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.09 0 0 0 0 0 0 0
SpindlePole 0.05 0 – 0 0.15 0.09 0.18 0.22 0.07 0.16 0.1 0.12 – 0.05 0 0.22 0.25 0.19 0 0 0 0 0 0
Vac/Vac Membrane 0 0.12 – 0 0.11 0.06 0.06 0.07 0 0.05 0 0 – 0 0 0.2 0.14 0.12 0 0.08 0.13 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 – 2 2 0 0 1 1 0 0 1 – 3 0 15 5 8 4 5 0 0 2 4
Bud 6 3 – 2 6 3 1 9 10 6 20 14 – 1 1 16 8 5 0 0 0 0 0 1
Bud Neck 1 0 – 2 3 0 2 4 3 3 2 3 – 0 0 2 2 2 0 2 0 0 0 4
Bud Site 0 0 – 0 0 0 3 2 4 4 6 0 – 0 0 0 1 0 – – – – – –
Cell Periphery 1 0 – 0 3 0 2 1 1 2 0 0 – 1 3 17 7 8 0 2 0 0 0 0
Cytoplasm 55 67 – 28 58 27 60 45 24 37 24 33 – 77 124 47 32 53 13 17 1 2 8 21
Endoplasmic Reticulum 6 8 – 4 3 1 5 6 7 0 2 5 – 5 0 14 8 12 4 2 0 1 1 0
Endosome 4 17 – 1 20 8 22 17 2 5 3 10 – 3 3 36 39 19 30 26 1 0 6 2
Golgi 1 1 – 0 0 0 0 0 0 0 0 0 – 0 0 2 2 3 2 4 0 0 1 0
Mitochondria 102 59 – 8 19 77 105 114 146 168 232 208 – 2 1 32 23 24 140 145 21 15 39 73
Nucleus 0 2 – 0 3 4 8 9 5 9 9 5 – 1 0 6 2 7 0 1 0 0 1 1
Nuclear Periphery 1 0 – 0 0 0 1 1 0 1 2 2 – 0 0 1 0 0 2 2 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 1 0 0 – 0 0 0 2 0 0 0 0 0 1 0
Peroxisomes 0 1 – 0 3 4 6 6 3 4 4 9 – 1 0 12 15 0 2 3 0 0 0 1
SpindlePole 8 5 – 1 19 10 41 51 13 37 25 31 – 5 1 50 41 31 7 6 0 0 2 3
Vac/Vac Membrane 5 19 – 1 14 7 13 16 7 11 2 11 – 2 2 46 23 19 6 20 4 0 1 8
Unique Cell Count 159 159 46 130 116 225 231 184 236 262 267 96 132 231 164 164 223 248 34 24 72 130
Labelled Cell Count 191 182 49 153 141 269 282 226 288 331 332 101 135 296 210 191 223 248 34 24 72 130


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.6 – 5.4 5.8 4.4 4.6 4.9 3.8 4.1 3.6 4.2 – 5.4 5.0 5.9 5.9 5.4 – 4.7 5.2
Std Deviation (1e-4) 1.0 1.0 – 1.7 1.7 1.3 1.4 1.4 1.1 1.0 1.1 1.4 – 1.4 1.0 1.2 1.3 1.1 – 1.4 1.3
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3671 0.4865 0.4091 0.7486 -0.418 0.3001 -0.3148 0.0711 0.1058 -0.0387 -0.0509 0.2472 -1.0004 -0.6312 -1.1569 -0.555 -0.745 -0.8475
Actin 0.0312 0.0056 0.0317 0.0169 0.016 0.0094 0.0262 0.0213 0.0179 0.0078 0.0048 0.0159 0.0269 0.0121 0.0274 0.0076 0.0318 0.0154
Bud 0.0033 0.0042 0.0066 0.0079 0.0016 0.0013 0.001 0.0029 0.0024 0.0022 0.0032 0.0008 0.0016 0.0042 0.0028 0.0047 0.0027 0.0009
Bud Neck 0.0034 0.002 0.0026 0.0149 0.0071 0.0059 0.0092 0.0031 0.0036 0.0027 0.0017 0.022 0.0021 0.002 0.0034 0.0094 0.0012 0.0068
Bud Periphery 0.0059 0.0066 0.0119 0.0119 0.0049 0.0034 0.002 0.0064 0.0054 0.0101 0.0092 0.0022 0.0024 0.0056 0.0091 0.0109 0.0103 0.0023
Bud Site 0.0104 0.0366 0.0154 0.0629 0.0028 0.0016 0.0123 0.0335 0.0133 0.0029 0.0083 0.0013 0.0045 0.0516 0.0118 0.0046 0.0044 0.003
Cell Periphery 0.0016 0.0016 0.0027 0.0012 0.0013 0.0007 0.0018 0.0026 0.0025 0.0014 0.0012 0.0013 0.0015 0.0027 0.0027 0.001 0.0013 0.0008
Cytoplasm 0.0475 0.1117 0.0655 0.0794 0.1206 0.0729 0.0897 0.1353 0.1081 0.0734 0.1058 0.1222 0.1104 0.1364 0.103 0.1159 0.1001 0.1329
Cytoplasmic Foci 0.1133 0.1202 0.1201 0.1214 0.1078 0.1687 0.1454 0.161 0.1218 0.1618 0.1066 0.1322 0.1441 0.1429 0.1278 0.1379 0.1131 0.1619
Eisosomes 0.0021 0.0013 0.0015 0.0011 0.0008 0.0006 0.0015 0.0011 0.0015 0.0004 0.0004 0.0006 0.004 0.0016 0.0023 0.0002 0.0009 0.0016
Endoplasmic Reticulum 0.0067 0.0046 0.0083 0.0091 0.0063 0.0059 0.0173 0.0071 0.0053 0.0048 0.0043 0.0086 0.0087 0.0077 0.0059 0.0026 0.0049 0.0091
Endosome 0.1551 0.1825 0.1371 0.1504 0.189 0.1193 0.113 0.1105 0.1028 0.1995 0.1446 0.1086 0.1538 0.1287 0.1496 0.2319 0.1282 0.1486
Golgi 0.0482 0.0492 0.044 0.0267 0.05 0.039 0.029 0.0436 0.0255 0.0487 0.018 0.0423 0.0355 0.0434 0.05 0.0346 0.0415 0.0292
Lipid Particles 0.0812 0.0309 0.0335 0.021 0.0362 0.0472 0.064 0.0118 0.0341 0.0207 0.0193 0.022 0.0539 0.0145 0.0216 0.0247 0.0335 0.0234
Mitochondria 0.1863 0.1438 0.2062 0.27 0.2812 0.2548 0.1052 0.1674 0.2332 0.1678 0.2631 0.2763 0.1372 0.1104 0.2193 0.1891 0.1489 0.2567
None 0.2079 0.2353 0.2245 0.0874 0.0754 0.1623 0.2816 0.2235 0.239 0.2001 0.1884 0.1825 0.2493 0.2921 0.2057 0.1586 0.2352 0.1311
Nuclear Periphery 0.009 0.0045 0.0066 0.0154 0.004 0.0065 0.0086 0.0009 0.0019 0.0011 0.0024 0.0041 0.0051 0.0018 0.0027 0.001 0.0283 0.003
Nucleolus 0.0028 0.0016 0.0021 0.0012 0.0015 0.0008 0.0059 0.0013 0.0014 0.0007 0.0023 0.0011 0.0039 0.0014 0.0019 0.0009 0.0029 0.0006
Nucleus 0.0042 0.0032 0.0038 0.0062 0.0058 0.0028 0.0033 0.0015 0.0013 0.0013 0.0028 0.004 0.0049 0.002 0.0033 0.0013 0.0084 0.0012
Peroxisomes 0.06 0.0336 0.0508 0.0582 0.0607 0.0815 0.064 0.0478 0.0608 0.0687 0.0396 0.0361 0.0202 0.016 0.025 0.0386 0.0656 0.0468
Punctate Nuclear 0.0049 0.0029 0.005 0.0032 0.0022 0.0058 0.0044 0.0012 0.0018 0.0028 0.0046 0.0015 0.0112 0.0013 0.0052 0.0038 0.0202 0.0028
Vacuole 0.0101 0.0138 0.0142 0.0208 0.0153 0.0061 0.0111 0.0129 0.0121 0.0134 0.0571 0.0092 0.0145 0.0179 0.0142 0.0139 0.0109 0.0155
Vacuole Periphery 0.0047 0.0041 0.0059 0.0127 0.0095 0.0035 0.0034 0.0031 0.0042 0.0077 0.0125 0.0052 0.0045 0.0039 0.0053 0.0071 0.006 0.0065

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.6092 9.4787 10.1108 8.8712 5.1554 7.2857 11.5306 10.107 7.7026 10.3584
Translational Efficiency 0.6936 0.7892 0.5025 0.4688 1.1115 0.908 0.558 0.5389 0.5735 0.6997

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2386 1185 68 1023 1546 1101 1760 344 3932 2286 1828 1367

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.69 684.48 873.94 831.32 690.48 698.62 862.78 870.46 651.77 691.29 863.20 841.17
Standard Deviation 65.89 90.27 116.77 117.07 83.54 104.22 101.94 122.37 79.68 97.50 102.56 119.64
Intensity Change Log 2 — 0.127256 0.479782 0.407652 — 0.016908 0.321393 0.334178 — 0.070464 0.398924 0.369603

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000313 0.001735 0.015094 0.023290 0.001003 0.000693 0.002852 0.003611 0.000584 0.001233 0.003308 0.018338
Bud Neck 0.064373 0.033876 0.013233 0.037329 0.022567 0.034532 0.032085 0.027842 0.047936 0.034192 0.031383 0.034942
Bud Site 0.019314 0.027403 0.067209 0.116666* 0.015216 0.022884 0.033707 0.068110 0.017703 0.025227 0.034954 0.104447*
Cell Periphery 0.000256 0.000455 0.000722 0.000372 0.000190 0.000295 0.000140 0.000297 0.000230 0.000378 0.000162 0.000353
Cytoplasm 0.143403* 0.058926 0.142770* 0.176599* 0.076902 0.057015 0.231141* 0.160492* 0.117256* 0.058006 0.227854* 0.172546*
Cytoplasmic Foci 0.291382* 0.213047* 0.142205 0.146353 0.348943* 0.212303* 0.188181* 0.178587 0.314014* 0.212688* 0.186471* 0.154465
Eisosomes 0.000240 0.000330 0.000280 0.000274 0.000212 0.000231 0.000111 0.000143 0.000229 0.000283 0.000118 0.000241
Endoplasmic Reticulum 0.003666 0.000861 0.026914 0.004175 0.001275 0.001341 0.004748 0.003729 0.002726 0.001092 0.005572 0.004063
Endosome 0.071355 0.064022 0.081479 0.025749 0.068857 0.125022 0.043573 0.050356 0.070373 0.093401 0.044983 0.031941
Golgi 0.033646 0.092565 0.016318 0.019250 0.097144 0.067182 0.011915 0.013127 0.058613 0.080340 0.012079 0.017709
Lipid Particles 0.027796 0.011630 0.028772 0.004568 0.020843 0.016686 0.010394 0.006807 0.025062 0.014065 0.011077 0.005131
Mitochondria 0.168937* 0.345957* 0.029808 0.025810 0.205550* 0.260312* 0.021443 0.040763 0.183333* 0.304708* 0.021755 0.029573
Mitotic Spindle 0.004090 0.006415 0.076353 0.072483 0.008840 0.019035 0.082959 0.080691 0.005958 0.012493 0.082713 0.074548
None 0.003707 0.000789 0.001739 0.000765 0.001428 0.001162 0.001614 0.001010 0.002811 0.000969 0.001619 0.000827
Nuclear Periphery 0.000832 0.000159 0.001664 0.000626 0.000355 0.000765 0.003023 0.001247 0.000644 0.000451 0.002973 0.000782
Nuclear Periphery Foci 0.000489 0.000156 0.017315 0.001405 0.000427 0.000664 0.002495 0.002426 0.000465 0.000401 0.003046 0.001662
Nucleolus 0.003785 0.001331 0.005949 0.000836 0.002509 0.002239 0.003956 0.001225 0.003284 0.001768 0.004031 0.000934
Nucleus 0.044956 0.011898 0.109518 0.050817 0.018179 0.017548 0.120006 0.054594 0.034428 0.014619 0.119616 0.051767
Peroxisomes 0.024849 0.099383 0.078048 0.145705* 0.070930 0.068249 0.053337 0.075150 0.042967 0.084388 0.054256 0.127950*
Vacuole 0.078085 0.019926 0.127858 0.143128 0.029489 0.073166 0.147153 0.223594* 0.058978 0.045568 0.146435 0.163377
Vacuole Periphery 0.014525 0.009137 0.016752 0.003801 0.009141 0.018676 0.005164 0.006199 0.012408 0.013731 0.005595 0.004405

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.10 -4.03 -10.22 -9.31 -1.08 1.90 -5.76 -5.27 -6.12 -1.01 -4.04 -8.51 -10.45 -9.92 -8.28
Bud Neck 8.38 16.02 8.95 -0.24 -7.38 -4.01 -3.84 -0.43 2.69 2.06 5.51 8.03 6.46 0.86 -0.77
Bud Site -3.56 -3.84 -15.82 -13.32 -2.56 -3.39 -7.32 -6.40 -4.92 -3.72 -4.68 -7.31 -17.13 -14.58 -12.60
Cell Periphery -4.21 -4.23 -1.87 2.75 3.57 -2.32 2.22 -0.82 1.00 -2.05 -4.43 3.45 -2.46 2.06 -6.06
Cytoplasm 15.87 -0.11 -5.70 -16.68 -1.66 3.71 -22.65 -7.95 -9.58 4.73 15.39 -17.61 -10.26 -19.61 5.35
Cytoplasmic Foci 10.65 9.91 25.81 12.11 0.72 16.60 25.26 20.37 6.55 2.77 18.48 26.39 34.30 14.42 7.00
Eisosomes -3.28 -0.96 -0.28 3.48 0.84 -0.74 10.63 5.84 3.45 -2.73 -2.69 8.41 0.53 3.28 -8.69
Endoplasmic Reticulum 5.17 -4.48 -2.09 -8.88 4.21 -0.20 -12.46 -6.62 -5.84 1.96 4.53 -7.26 -4.55 -9.83 2.44
Endosome 1.94 -0.24 18.74 12.50 4.03 -10.51 9.80 7.30 15.12 0.29 -6.94 12.71 20.38 21.53 6.53
Golgi -13.60 4.89 8.98 17.91 0.28 6.12 23.24 21.94 15.94 0.58 -6.83 24.91 20.86 22.92 -1.49
Lipid Particles 9.36 0.02 16.37 6.28 3.69 2.01 7.19 9.20 6.20 3.76 8.07 12.37 18.99 9.25 7.50
Mitochondria -16.10 9.73 28.33* 32.32* 0.52 -4.96 27.75 20.35 22.38 -1.11 -15.64 39.73* 36.14* 40.42* -0.65
Mitotic Spindle -2.03 -3.12 -11.77 -11.16 0.71 -4.07 -16.11 -6.40 -5.18 1.12 -4.92 -17.38 -13.23 -11.45 2.23
None 5.02 2.79 4.89 -0.65 2.12 0.84 -0.50 2.01 1.18 2.77 4.88 2.91 5.25 0.57 3.17
Nuclear Periphery 5.71 -1.99 -1.64 -8.71 1.57 -2.37 -12.06 -5.72 -3.15 5.59 1.81 -10.89 -4.90 -6.25 8.43
Nuclear Periphery Foci 4.91 -2.49 -7.51 -9.87 2.30 -1.39 -9.36 -4.78 -4.22 -0.53 0.78 -7.80 -8.80 -8.82 2.73
Nucleolus 5.46 -1.14 7.61 1.17 2.93 0.32 -2.46 2.53 2.96 7.41 4.10 -1.74 7.40 3.14 8.69
Nucleus 13.16 -3.20 -6.40 -14.43 1.98 0.17 -24.79 -7.61 -7.52 7.40 10.51 -21.88 -10.50 -15.87 11.23
Peroxisomes -15.38 -2.85 -13.22 -1.34 -2.05 0.57 5.92 2.23 1.77 -0.95 -12.15 -1.82 -10.02 -1.95 -8.39
Vacuole 18.32 -3.09 -17.18 -25.29 -4.05 -10.09 -29.30 -18.92 -15.52 -9.06 4.55 -23.28 -25.47 -26.94 -8.64
Vacuole Periphery 4.96 -0.05 13.33 6.19 2.93 -6.63 6.44 4.12 8.68 0.24 -1.45 11.89 13.37 11.53 2.29
Standard name
Human Ortholog
Description Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
Localization
Cell Percentages mitochondrion (57%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Mih1

Mih1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mih1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available