Standard name
Description Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; relative distribution to nucleus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.86 0.79 0.2 0.48 0.42 0.43 0.2 0.26 0.16 0.27 0 0.62 0.33 0.54 0.69 0.74 0.96 0.77 0.75 0.42 0.77 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.15 0.34 0.34 0.46 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.05 0.1 0.62 0.25 0.28 0.3 0.43 0.36 0.39 0.32 0.84 0.17 0.39 0.13 0.08 0.05 0 0.11 0.11 0.21 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.05 0 0.11 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Vac/Vac Membrane 0 0.1 0.11 0.17 0.27 0.24 0.19 0.17 0.16 0.16 0.25 0.07 0.22 0.26 0.37 0.31 0.23 0 0.05 0 0.11 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 2 0 0 0 0 0 1 0 0 0 1 0 0 1 1 0 0 2 3
Bud 0 2 1 0 0 2 6 5 9 7 8 0 0 0 0 0 0 0 4 4 14 10 10
Bud Neck 0 0 1 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 3 1 2 3 3
Bud Site 0 0 0 0 0 1 1 5 1 2 2 0 0 0 0 0 0
Cell Periphery 1 1 13 1 3 2 5 2 1 1 1 0 3 0 7 4 10 0 0 0 0 0 0
Cytoplasm 185 285 351 63 252 168 234 112 140 88 145 15 263 139 169 159 182 186 287 372 91 319 343
Endoplasmic Reticulum 1 2 4 0 3 1 6 2 1 0 0 0 0 1 10 8 6 0 0 0 3 12 10
Endosome 0 2 0 6 23 16 15 5 3 3 22 3 23 20 13 5 3 0 3 5 6 4 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 2 2 0 0 1
Mitochondria 2 7 0 8 13 52 82 189 183 256 112 16 3 6 4 1 0 0 5 8 3 7 4
Nucleus 4 16 43 196 133 112 165 237 198 214 173 305 73 163 42 18 12 1 39 55 45 14 11
Nuclear Periphery 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 1 1 4 2 20 14 26 23 40 2 13 0 1 1 0 0 6 7 4 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 1 0 1 0 0 1 1 1 4 0 0 0 0 0 0 0 2 12 13 6 4
Vac/Vac Membrane 0 34 50 55 144 94 102 93 88 89 136 25 95 107 115 72 58 1 18 21 25 22 24
Unique Cell Count 190 332 445 318 529 398 541 551 543 553 544 365 424 416 313 231 247 194 372 499 219 415 431
Labelled Cell Count 193 350 465 331 576 452 620 671 639 688 628 404 462 449 363 268 273 194 372 499 219 415 431


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.4 7.5 6.9 7.4 6.1 6.2 5.5 5.2 4.8 5.7 7.5 7.3 7.3 9.6 9.9 9.7 6.8 6.7 7.4
Std Deviation (1e-4) 0.9 1.1 0.8 1.0 1.1 1.1 1.1 1.0 0.9 0.8 0.9 1.8 1.5 1.3 2.1 1.7 1.6 1.1 1.6 2.9
Intensity Change (Log2) -0.12 -0.03 -0.3 -0.27 -0.44 -0.52 -0.66 -0.4 -0.01 -0.04 -0.03 0.36 0.4 0.36 -0.14 -0.17 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.6 -2.9 -2.7 -2.3 -3.3 -3.6 0 -3.6 -3.3 -2.4 -3.5 -0.6 -0.9 0.8
Cytoplasm -16.1 -10.0 -10.9 -11.3 -18.4 -16.6 -19.9 -16.3 -21.3 -5.5 -13.5 -7.3 -2.9 -1.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.3 0 0
Endosome 0 4.5 4.3 3.5 0 0 0 4.3 0 5.0 4.7 4.3 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.3 7.9 8.6 13.7 13.6 0 10.2 4.5 0 0 0 0 0
Nucleus 15.3 6.3 6.9 8.0 11.6 9.8 10.4 8.4 21.1 3.3 10.1 1.6 -0.8 -2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 4.1 3.4 0 4.4 7.2 0 3.8 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.4 6.2 4.8 3.3 2.5 2.2 2.2 5.5 -2.1 4.4 5.5 8.4 6.4 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0055 3.8106 3.5088 3.0571 3.1196 3.3475 3.7862 5.3628 4.6566 4.4095 4.3594 4.6863 4.2463 5.1124 4.7479 4.3088 3.6164 4.5026
Actin 0.0503 0.0032 0.0034 0.0149 0.0212 0.001 0.0319 0.0021 0.0105 0.0001 0.0295 0.001 0.0067 0.0008 0.0009 0.0003 0.0005 0.0006
Bud 0.0023 0.0039 0.001 0.0023 0.0017 0.0047 0.0015 0.0042 0.003 0.0004 0.0002 0.0016 0.0004 0.0001 0.0001 0.0007 0.0002 0.0039
Bud Neck 0.0015 0.0015 0.0031 0.0006 0.0017 0.0105 0.0025 0.0016 0.0009 0.0008 0.0007 0.0079 0.0002 0.0002 0.0004 0.0022 0.0007 0.0073
Bud Periphery 0.0029 0.0015 0.0012 0.0013 0.0013 0.004 0.001 0.0021 0.0028 0.0001 0.0001 0.0008 0.0002 0 0 0.0003 0 0.0033
Bud Site 0.0063 0.0379 0.0286 0.0071 0.0021 0.0021 0.021 0.0255 0.005 0.0008 0.0004 0.0008 0.0008 0.0006 0.0004 0.0002 0.0001 0.0016
Cell Periphery 0.0005 0.0004 0.0005 0.0001 0.0001 0.0003 0.0004 0.0005 0.0005 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0003
Cytoplasm 0.2851 0.544 0.3733 0.3667 0.4021 0.4511 0.3717 0.4715 0.4016 0.4364 0.3595 0.3926 0.5081 0.9928 0.7029 0.545 0.9324 0.7575
Cytoplasmic Foci 0.0529 0.02 0.0136 0.0691 0.0337 0.0024 0.0565 0.0035 0.0075 0.0008 0.0064 0.0063 0.024 0.0008 0.0011 0.0019 0.0011 0.0031
Eisosomes 0.0005 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0089 0.0035 0.0046 0.0031 0.0045 0.0025 0.0063 0.0021 0.004 0.0005 0.001 0.0028 0.0161 0.0007 0.0023 0.0015 0.0018 0.0142
Endosome 0.0568 0.0071 0.0245 0.0534 0.0281 0.002 0.0336 0.0019 0.0183 0.0003 0.0023 0.0022 0.3252 0.0002 0.0003 0.0013 0.0004 0.0021
Golgi 0.015 0.0011 0.0075 0.0249 0.022 0.0002 0.0111 0.0004 0.0019 0 0.0078 0.0002 0.093 0 0.0001 0.0001 0 0.0002
Lipid Particles 0.0198 0.0012 0.0089 0.0274 0.0025 0.0001 0.0168 0.0001 0.0022 0 0.0071 0.0001 0.0038 0 0 0 0 0.0001
Mitochondria 0.0259 0.001 0.0063 0.0194 0.01 0.0007 0.0037 0.0005 0.0052 0.0003 0.0011 0.0127 0.0055 0 0.0002 0.0002 0.0001 0.0003
None 0.015 0.0031 0.0041 0.002 0.0024 0.0011 0.0703 0.0068 0.0079 0.0006 0.0008 0.0139 0.002 0.0015 0.0034 0.0014 0.0034 0.0288
Nuclear Periphery 0.0202 0.0073 0.0058 0.0046 0.0107 0.0076 0.0108 0.0082 0.006 0.0044 0.0065 0.0059 0.0043 0.0004 0.0047 0.0055 0.0044 0.0047
Nucleolus 0.0121 0.0026 0.0039 0.0008 0.0011 0.0054 0.0027 0.0025 0.0024 0.0016 0.0034 0.0046 0 0 0.0002 0.0004 0 0.0006
Nucleus 0.3722 0.3391 0.4937 0.3539 0.3859 0.4899 0.3304 0.4257 0.5013 0.5509 0.5383 0.5378 0.0009 0.0015 0.2809 0.4336 0.0535 0.1642
Peroxisomes 0.029 0.0057 0.0035 0.0438 0.0614 0.0001 0.0076 0.0002 0.0029 0 0.0308 0.0001 0.0004 0 0 0 0 0.0001
Punctate Nuclear 0.0124 0.0073 0.0031 0.002 0.0053 0.0094 0.0145 0.0372 0.0069 0.0014 0.0031 0.0052 0.0004 0.0001 0.0019 0.0038 0.0002 0.0022
Vacuole 0.0071 0.0075 0.0084 0.0019 0.0016 0.0044 0.0046 0.0029 0.0069 0.0006 0.0006 0.003 0.0038 0.0001 0.0002 0.0015 0.0009 0.0046
Vacuole Periphery 0.0034 0.0009 0.0009 0.0007 0.0006 0.0006 0.0008 0.0003 0.0021 0.0001 0.0002 0.0004 0.0039 0 0 0.0002 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.0422 14.9239 10.7281 21.7011 19.616 10.9497 25.6994 25.7793 17.776 27.9583
Translational Efficiency 1.4883 1.827 1.7955 0.8763 1.2786 3.1763 1.1078 0.957 1.6233 1.1329

Hit Data

Dataset Hit
Protein Abundance
Protein Localization
Gene Expression
Translational Efficiency