Standard name
Human Ortholog
Description Transcription factor; involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.11 0.13 0.14 0.09 0.1 0 0 0 0.14 0.12 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0 0 0.11 0 0 0 0.13 0.05 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.85 0.92 0.88 0.89 0.84 0.83 0.81 0.77 0.68 0.75 0.74 0.72 0.92 0.82 0.82 0.79 0.81 0.8 0.91 0.9 0.84 0.69 0.6 0.49
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.07 0.08 0.11 0.11 0.19 0.21 0.22 0.42 0.33 0.37 0.39 0 0.09 0.08 0.08 0.09 0.07 0 0 0.08 0.08 0.08 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0.06 0.11 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Bud 0 0 0 1 2 2 2 1 4 2 3 3 0 0 0 0 0 1 1 1 4 2 2 3
Bud Neck 0 0 0 1 2 0 2 4 3 5 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 5 11 6 3 0 10 6 7 11 0 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 7 13 19 13 16 24 36 16 11 13 11 10 41 36 39 22 27 0 1 0 34 43 33
Endoplasmic Reticulum 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 5 2 6 0 0 0 0 3 1
Endosome 0 0 3 5 1 2 7 1 1 1 0 1 1 0 1 0 3 3 0 3 5 0 4 8
Golgi 0 0 2 0 0 0 0 1 0 0 0 0 0 0 1 0 1 0 0 1 3 0 2 1
Mitochondria 3 34 3 9 56 21 16 12 90 34 53 43 0 3 1 9 5 3 0 4 3 0 4 6
Nucleus 58 343 386 403 441 597 637 600 475 508 493 481 158 315 226 226 204 211 76 357 427 172 221 159
Nuclear Periphery 0 8 1 3 7 13 12 6 12 10 5 6 0 1 0 3 6 2 0 0 1 0 3 5
Nucleolus 15 25 36 50 58 140 163 167 296 224 249 259 7 33 22 22 22 18 2 10 39 18 30 41
Peroxisomes 0 0 0 1 0 0 2 0 0 0 0 0 1 1 1 0 0 1 0 0 0 0 0 0
SpindlePole 0 1 3 1 3 8 2 12 4 7 2 2 0 5 3 1 0 2 0 4 1 1 5 4
Vac/Vac Membrane 0 6 11 2 12 8 14 19 9 3 11 8 1 3 7 6 13 12 2 11 12 15 41 49
Unique Cell Count 68 374 437 454 522 721 788 775 703 675 670 667 171 382 277 287 252 264 84 398 508 249 369 323
Labelled Cell Count 78 424 460 500 606 813 884 860 921 811 836 826 178 404 298 311 278 287 84 398 508 249 369 323


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.4 6.5 8.9 8.0 6.5 7.3 7.5 8.0 6.8 7.5 7.6 8.3 9.3 8.6 8.0 9.9 12.3 12.9 11.2 11.5 11.3
Std Deviation (1e-4) 1.8 1.4 2.1 1.7 1.5 1.9 2.1 1.9 1.8 1.8 2.5 2.7 2.3 2.2 2.2 3.0 3.1 3.9 7.4 8.5 7.8
Intensity Change (Log2) – – – -0.15 -0.45 -0.28 -0.24 -0.16 -0.4 -0.24 -0.23 -0.11 0.06 -0.06 -0.16 0.15 0.46 0.53 0.33 0.36 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 2.6 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 -0.5 -0.8 0.1 1.4 -0.7 -1.5 -1.1 -1.5 1.7 4.5 5.2 5.4 3.3 4.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.7 6.4 2.6 1.8 1.3 7.3 4.0 5.4 4.7 0 0 0 0 0 0
Nucleus 0.2 -1.7 -2.5 -3.4 -4.7 -7.9 -5.4 -5.9 -6.4 1.5 -2.4 -2.5 -3.5 -2.7 -3.0
Nuclear Periphery 0 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.4 1.5 5.1 5.7 6.0 12.2 9.6 10.8 11.2 -1.8 0.2 -0.1 -0.3 0.2 -0.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1.3 0 0 0 0 0 0 0 0 0 0
Vacuole -2.6 -0.2 -1.8 -0.9 -0.1 -1.5 -3.0 -1.0 -1.6 0 -1.9 0 -0.4 1.8 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.8852 9.3968 9.1359 8.5716 7.7517 9.0762 11.1333 12.7782 11.963 11.0119 10.7593 11.4379 12.8056 11.0198 11.1171 10.2947 10.8937 10.4103
Actin 0.006 0 0.0088 0.0003 0.0027 0.001 0.004 0 0.0004 0.0017 0.141 0.0004 0 0 0 0 0 0
Bud 0.0006 0 0.0003 0.0001 0.0002 0.0001 0.0028 0 0.0001 0.0001 0.0003 0 0 0 0 0 0 0
Bud Neck 0.0016 0 0.0078 0.0002 0.0004 0.0005 0.0028 0 0.0001 0.0001 0.0003 0.0005 0 0 0 0 0.0001 0.0003
Bud Periphery 0.0018 0.0001 0.0004 0.0001 0.0004 0.0001 0.006 0 0.0001 0.0001 0.0003 0.0001 0 0 0 0 0 0
Bud Site 0.0065 0.0001 0.0027 0.0014 0.001 0.0001 0.0038 0 0.0005 0.0003 0.0007 0.0001 0 0 0 0 0 0
Cell Periphery 0.0007 0 0.0001 0.0001 0.0001 0 0.0013 0 0 0 0.0001 0.0001 0 0 0 0 0 0
Cytoplasm 0.0014 0 0.0004 0.0037 0.0001 0.0003 0.0028 0 0.0022 0.0002 0.008 0.0001 0 0 0 0 0 0
Cytoplasmic Foci 0.0133 0 0.0033 0.0026 0.0097 0.0062 0.016 0 0.0199 0.0458 0.023 0.001 0 0 0 0 0 0
Eisosomes 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0 0 0.0003 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0057 0 0.0005 0.0011 0.0004 0.0001 0.0008 0 0.001 0.0001 0.0085 0 0 0 0 0 0 0
Endosome 0.0102 0 0.0056 0.0122 0.0233 0.0017 0.03 0 0.0049 0.0028 0.05 0.0009 0 0 0 0 0 0
Golgi 0.0063 0 0.0024 0.001 0.0129 0.0015 0.0071 0 0.0014 0.0031 0.0208 0.001 0 0 0 0 0 0
Lipid Particles 0.0234 0 0.0068 0.0044 0.0078 0.0116 0.0447 0 0.0015 0.0092 0.0621 0.0014 0 0 0 0 0 0
Mitochondria 0.0183 0.0004 0.0009 0.003 0.0195 0.0016 0.0382 0.0001 0.0003 0.0005 0.01 0.0093 0.0001 0 0 0.0001 0.0002 0.0001
None 0.0008 0 0.0003 0.0073 0.0001 0.0004 0.0007 0 0.0015 0.0002 0.014 0 0 0 0 0 0 0
Nuclear Periphery 0.017 0.0003 0.0008 0.003 0.0019 0.0005 0.0021 0.0005 0.0038 0.0006 0.0171 0.0001 0.0003 0.0004 0.0002 0.0001 0.0002 0.0001
Nucleolus 0.0476 0.0209 0.0098 0.0354 0.2396 0.1123 0.0536 0.0091 0.0201 0.0341 0.0778 0.0819 0.0186 0.0301 0.0193 0.022 0.1734 0.0802
Nucleus 0.7992 0.9779 0.9444 0.9154 0.6361 0.8509 0.7566 0.9901 0.93 0.8816 0.5468 0.902 0.9807 0.9693 0.9774 0.9776 0.8244 0.9191
Peroxisomes 0.0216 0 0.0033 0.0005 0.0358 0.005 0.0046 0 0.0014 0.0168 0.0062 0.0004 0 0 0 0 0 0
Punctate Nuclear 0.0097 0.0001 0.0008 0.0057 0.0039 0.0057 0.0024 0.0002 0.0103 0.0026 0.0104 0.0001 0.0002 0.0002 0.003 0.0001 0.0015 0.0001
Vacuole 0.0045 0 0.0003 0.002 0.0019 0.0002 0.0139 0 0.0003 0.0001 0.0009 0.0003 0 0 0 0 0 0.0001
Vacuole Periphery 0.0038 0 0.0001 0.0006 0.0022 0.0001 0.006 0 0.0002 0.0001 0.0015 0.0002 0 0 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.0677 38.951 31.4536 45.0753 57.3242 34.5824 30.9854 38.308 32.4432 38.0182
Translational Efficiency 1.9222 1.5115 1.8737 1.1073 0.9461 1.1717 1.5817 1.3695 1.7934 1.1727

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2232 1482 857 1536 1230 2255 2141 1372 3462 3737 2998 2908

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 915.05 976.50 1432.90 1338.35 805.84 1095.53 1467.31 1508.34 876.25 1048.33 1457.47 1418.55
Standard Deviation 137.06 187.25 235.11 297.28 137.70 174.53 270.41 305.28 146.90 188.88 261.27 312.81
Intensity Change Log 2 — 0.093769 0.647015 0.548533 — 0.443064 0.864608 0.904396 — 0.267890 0.753002 0.726131

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000393 0.000768 0.000624 0.000982 0.000342 0.000519 0.000422 0.000769 0.000375 0.000618 0.000479 0.000881
Bud Neck 0.007144 0.011580 0.009811 0.023455 0.006395 0.012958 0.006715 0.018306 0.006878 0.012411 0.007600 0.021026
Bud Site 0.000612 0.001437 0.000629 0.004241 0.000335 0.001488 0.000323 0.002749 0.000514 0.001468 0.000410 0.003537
Cell Periphery 0.000050 0.000139 0.000021 0.000368 0.000046 0.000104 0.000028 0.000195 0.000049 0.000118 0.000026 0.000286
Cytoplasm 0.000750 0.001319 0.000028 0.002760 0.000180 0.000594 0.000024 0.000681 0.000548 0.000882 0.000025 0.001779
Cytoplasmic Foci 0.000280 0.000536 0.000030 0.002183 0.000015 0.000179 0.000019 0.000386 0.000186 0.000321 0.000022 0.001335
Eisosomes 0.000030 0.000051 0.000086 0.000051 0.000026 0.000058 0.000079 0.000055 0.000029 0.000055 0.000081 0.000053
Endoplasmic Reticulum 0.002529 0.003218 0.000528 0.001467 0.002446 0.002955 0.000477 0.000903 0.002499 0.003060 0.000492 0.001201
Endosome 0.000304 0.000519 0.000011 0.002566 0.000139 0.000626 0.000009 0.000770 0.000245 0.000584 0.000010 0.001719
Golgi 0.000169 0.000346 0.000038 0.002137 0.000065 0.000179 0.000017 0.000940 0.000132 0.000246 0.000023 0.001573
Lipid Particles 0.000345 0.000217 0.000005 0.000285 0.000027 0.000112 0.000003 0.000043 0.000232 0.000153 0.000003 0.000171
Mitochondria 0.000768 0.001514 0.001312 0.003530 0.000729 0.001326 0.000870 0.003517 0.000754 0.001401 0.000997 0.003524
Mitotic Spindle 0.000835 0.002803 0.000068 0.014933 0.000374 0.003734 0.000040 0.003893 0.000671 0.003365 0.000048 0.009724
None 0.001487 0.004604 0.000465 0.004811 0.002111 0.003347 0.000370 0.001458 0.001709 0.003845 0.000397 0.003229
Nuclear Periphery 0.000391 0.000265 0.000038 0.001891 0.000189 0.000647 0.000043 0.000537 0.000319 0.000495 0.000042 0.001252
Nuclear Periphery Foci 0.000751 0.000527 0.000003 0.001917 0.000186 0.000340 0.000004 0.000388 0.000550 0.000414 0.000004 0.001196
Nucleolus 0.079549 0.076986 0.036721 0.031089 0.072657 0.060998 0.022292 0.037010 0.077100 0.067339 0.026417 0.033883
Nucleus 0.902697* 0.891241* 0.947507* 0.894889* 0.913159* 0.907089* 0.967345* 0.923948* 0.906414* 0.900804* 0.961674* 0.908599*
Peroxisomes 0.000452 0.000929 0.001797 0.001087 0.000258 0.000612 0.000622 0.000856 0.000383 0.000738 0.000958 0.000978
Vacuole 0.000247 0.000580 0.000165 0.003267 0.000120 0.001041 0.000163 0.001624 0.000202 0.000858 0.000164 0.002492
Vacuole Periphery 0.000216 0.000421 0.000112 0.002089 0.000202 0.001094 0.000135 0.000973 0.000211 0.000827 0.000129 0.001562

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.89 -9.90 -10.40 -4.45 -6.53 -6.29 -5.32 -9.79 -5.74 -8.29 -10.71 -8.04 -13.96 -7.66 -11.46
Bud Neck -5.01 -4.72 -10.61 -7.55 -9.00 -7.64 -1.38 -11.41 -5.43 -11.14 -8.57 -2.45 -14.96 -9.08 -14.42
Bud Site -3.61 -0.16 -6.38 -4.86 -6.47 -5.53 -0.12 -4.30 -2.25 -4.30 -5.71 1.33 -7.48 -5.03 -7.85
Cell Periphery -3.18 3.11 -3.14 -2.01 -3.50 -3.58 2.36 -2.46 -1.37 -2.78 -4.54 3.27 -3.93 -2.53 -4.40
Cytoplasm -0.86 1.97 -2.75 -1.63 -4.12 -2.82 4.91 -3.15 -0.99 -3.89 -1.00 2.19 -3.09 -2.22 -4.98
Cytoplasmic Foci -0.81 2.13 -3.23 -2.48 -3.87 -2.63 -0.80 -2.65 -1.32 -2.62 -0.91 2.18 -3.59 -3.01 -4.41
Eisosomes -6.55 -18.46 -5.16 0.29 8.23 -12.53 -13.25 -8.67 0.82 5.00 -13.42 -17.75 -9.24 1.04 7.97
Endoplasmic Reticulum -3.42 17.33 5.24 7.53 -6.59 -2.84 15.35 9.65 11.71 -5.24 -4.12 23.08 9.93 12.68 -8.42
Endosome -1.08 3.66 -3.83 -3.26 -4.60 -3.26 3.98 -2.49 -0.15 -3.13 -2.67 4.42 -4.38 -2.95 -5.45
Golgi -1.23 2.45 -2.40 -2.11 -2.63 -1.65 9.40 -1.71 -1.42 -1.81 -1.46 3.19 -2.91 -2.60 -3.21
Lipid Particles 0.57 1.86 0.50 -0.24 -4.38 -2.37 4.47 -2.17 1.66 -4.79 0.60 1.94 0.63 -0.06 -5.09
Mitochondria -3.79 -4.45 -8.50 -5.78 -6.98 -3.05 -1.34 -5.75 -4.39 -5.55 -4.55 -2.97 -9.60 -7.03 -8.99
Mitotic Spindle -1.84 -0.03 -5.99 -4.71 -5.63 -3.49 1.56 -4.55 -1.93 -4.83 -3.66 1.21 -7.40 -4.94 -7.67
None -3.04 5.47 -3.43 0.24 -4.82 -1.60 6.12 1.55 2.37 -5.32 -3.47 8.35 -2.79 1.16 -6.02
Nuclear Periphery 0.86 2.61 -3.41 -3.93 -4.61 -2.80 5.43 -4.46 0.29 -6.23 -1.31 3.15 -4.02 -3.13 -5.81
Nuclear Periphery Foci 0.72 2.90 -1.63 -2.18 -3.52 -1.74 4.77 -1.88 -0.24 -3.62 0.68 3.28 -1.63 -2.31 -4.19
Nucleolus 0.77 11.59 11.96 10.24 0.15 3.09 14.42 5.05 2.97 -7.63 3.92 21.98 12.05 9.07 -6.78
Nucleus 2.38 -10.24 2.33 0.52 8.94 1.43 -14.51 1.46 0.37 12.14 1.73 -21.11 2.42 1.11 16.19
Peroxisomes -4.39 -7.32 -2.85 -0.83 2.83 -5.59 -5.23 -9.26 -4.13 -3.62 -5.22 -7.09 -5.31 -2.62 -0.48
Vacuole -1.96 1.35 -4.80 -4.30 -4.93 -3.92 -1.44 -3.77 -1.24 -3.65 -4.09 0.97 -5.89 -4.18 -6.01
Vacuole Periphery -1.86 2.03 -3.00 -2.56 -3.22 -2.92 1.19 -2.96 0.57 -3.31 -3.24 2.09 -3.88 -1.47 -4.20
Standard name
Human Ortholog
Description Transcription factor; involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mcm1

Mcm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available