Standard name
Human Ortholog
Description Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.11 0.17 0.08 0 0.05 0 0.07 0.05 0 0.1 0.09 0.12 0.18 0.17 0.17 0 0 0.05 0.05 0.06 0.05
Cytoplasm 0.69 0.82 0.77 0.77 0.81 0.61 0.69 0.61 0.51 0.55 0.58 0.91 0.93 0.91 0.76 0.74 0.78 0.31 0.47 0.27 0.23 0.25 0.26
Endoplasmic Reticulum 0.22 0.11 0.18 0.07 0.06 0.09 0 0 0.07 0 0 0.05 0.05 0.06 0 0 0 0.46 0.22 0.39 0.49 0.46 0.48
Endosome 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.37 0.14 0.4 0.41 0.5 0.38 0 0 0 0 0 0 0.09 0.1 0.15 0.12 0.09 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0.05 0 0.19 0.14 0.11 0.07 0 0 0 0 0.12 0.17 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 14 4 10 5 4 0 0 3 0 2 4 4 1 8 5 5 14 27 12 14 33 25
Bud 4 7 0 0 2 5 4 3 3 0 7 2 3 2 0 1 3 4 9 2 2 11 12
Bud Neck 0 1 2 1 1 0 0 0 0 0 0 0 1 0 1 1 0 8 23 11 6 13 12
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 13 12 19 27 22 3 9 6 12 2 4 45 49 77 69 39 42 8 18 14 17 44 31
Cytoplasm 249 406 138 126 229 70 135 100 82 24 70 389 486 602 287 164 191 148 288 72 85 184 165
Endoplasmic Reticulum 79 53 33 12 17 10 2 5 11 0 4 20 25 42 9 8 4 220 133 104 184 337 302
Endosome 7 3 0 1 6 0 9 4 2 0 3 3 7 3 4 0 2 11 10 0 2 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 1 3 2 3 4 0 0 2 1 3
Mitochondria 11 15 0 3 8 42 27 65 65 22 46 1 4 1 7 3 6 41 61 41 44 62 36
Nucleus 2 2 0 0 1 2 1 2 4 1 5 0 0 0 7 3 2 0 1 0 0 0 1
Nuclear Periphery 1 0 0 0 0 0 0 0 2 0 1 0 0 3 0 0 0 2 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 1
Peroxisomes 0 0 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 2 2 0 0 0 2 0 0 2 0 0 0 0 1 2 3 3
Vac/Vac Membrane 17 15 1 3 15 3 38 23 17 3 4 7 10 9 47 38 20 11 26 5 7 23 23
Unique Cell Count 362 497 180 163 283 115 195 164 160 44 121 429 524 663 380 223 246 480 617 270 375 731 634
Labelled Cell Count 396 528 197 183 309 139 228 210 201 52 146 476 589 741 445 264 279 480 617 270 375 731 634


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.4 6.5 6.2 6.7 4.5 5.8 5.2 5.5 5.5 5.7 6.9 6.5 6.9 7.9 8.4 7.8 5.9 5.8 6.3
Std Deviation (1e-4) 0.7 0.8 0.9 1.3 1.3 1.3 1.4 1.4 1.4 1.6 2.0 1.2 1.2 1.3 1.5 1.7 1.7 1.5 1.3 1.6
Intensity Change (Log2) -0.06 0.05 -0.54 -0.16 -0.32 -0.24 -0.25 -0.18 0.08 0.0 0.08 0.28 0.37 0.27 -0.13 -0.16 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.8 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.6 -1.0 0 -2.2 -2.5 -1.0 0 -2.3 0 -0.5 0.4 2.3 2.0 1.9
Cytoplasm 0.1 1.1 -2.9 -1.6 -3.1 -4.9 -2.9 -3.5 4.6 5.9 5.1 -0.3 -0.7 0.2
Endoplasmic Reticulum -3.0 -4.2 0 -5.8 -4.5 -3.1 -3.1 -3.9 -5.5 -5.7 -5.0 -6.7 -4.9 -6.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 5.2 9.4 9.5 0 9.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 0 6.0 4.9 4.1 0 0 0 0 0 4.7 5.6 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2565 0.4149 0.5875 0.5824 0.2012 0.3383 1.7347 1.7693 1.9625 2.2158 1.4432 2.0283 4.3678 5.0281 4.8253 4.3458 4.7193 5.1398
Actin 0.0563 0.0028 0.0203 0.0549 0.053 0.0223 0.0114 0.0019 0.0063 0.0009 0.0146 0.0037 0.0023 0.0019 0.006 0.0019 0.0048 0.0016
Bud 0.004 0.0104 0.002 0.0006 0.0007 0.0009 0.003 0.0015 0.0022 0.0003 0.0144 0.0006 0.0008 0.0011 0.0038 0.001 0.0005 0.0002
Bud Neck 0.0071 0.0005 0.0038 0.001 0.0008 0.0063 0.0029 0.0007 0.0013 0.0005 0.001 0.0046 0.0032 0.0008 0.0021 0.0016 0.001 0.007
Bud Periphery 0.0055 0.0057 0.001 0.0013 0.0011 0.0023 0.0024 0.0025 0.0036 0.0007 0.0102 0.0008 0.0005 0.0019 0.0023 0.0017 0.0007 0.0006
Bud Site 0.0102 0.0186 0.0171 0.0009 0.0022 0.0005 0.0052 0.0133 0.0091 0.0004 0.0068 0.0011 0.0022 0.0172 0.0107 0.0007 0.0006 0.0007
Cell Periphery 0.0014 0.0016 0.0014 0.0007 0.0008 0.0008 0.0024 0.002 0.0016 0.0013 0.0011 0.0007 0.0018 0.0023 0.0012 0.0007 0.0006 0.0006
Cytoplasm 0.3674 0.4877 0.5376 0.4373 0.5527 0.5414 0.4777 0.5223 0.5576 0.4847 0.6621 0.5601 0.6274 0.6317 0.6161 0.6859 0.7168 0.6862
Cytoplasmic Foci 0.0319 0.013 0.0365 0.0174 0.0318 0.0216 0.0166 0.0196 0.0241 0.0067 0.0307 0.0242 0.019 0.0163 0.0146 0.0184 0.0156 0.0096
Eisosomes 0.0007 0.0003 0.0007 0.0006 0.0005 0.0002 0.0003 0.0003 0.0004 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.038 0.0238 0.0282 0.0386 0.0876 0.0299 0.0358 0.0197 0.0344 0.0342 0.0181 0.0345 0.0503 0.0418 0.0457 0.0415 0.032 0.0222
Endosome 0.0309 0.0068 0.0117 0.043 0.028 0.0111 0.0096 0.0141 0.0185 0.0121 0.0167 0.0115 0.0102 0.0128 0.0112 0.0133 0.0183 0.0054
Golgi 0.0059 0.0013 0.0028 0.0077 0.0078 0.0021 0.0016 0.0019 0.0034 0.0026 0.0032 0.0013 0.0014 0.0016 0.0012 0.0009 0.0027 0.0007
Lipid Particles 0.0081 0.0054 0.0057 0.026 0.0243 0.0039 0.0027 0.0015 0.0024 0.0003 0.001 0.0013 0.0032 0.0039 0.0013 0.003 0.0011 0.0005
Mitochondria 0.0093 0.0009 0.002 0.0059 0.0047 0.0015 0.0009 0.0024 0.0038 0.0032 0.0042 0.0032 0.0007 0.0027 0.0011 0.0007 0.0014 0.0008
None 0.3305 0.4049 0.3018 0.3247 0.1516 0.3407 0.4137 0.3847 0.3112 0.4477 0.1941 0.3402 0.2665 0.2485 0.2721 0.2055 0.1876 0.257
Nuclear Periphery 0.0319 0.0022 0.0052 0.0155 0.0138 0.0038 0.0036 0.0018 0.0039 0.0009 0.0038 0.0024 0.0022 0.0028 0.0022 0.0074 0.0027 0.0017
Nucleolus 0.0012 0.0005 0.0018 0.0005 0.0008 0.0003 0.0005 0.0004 0.0007 0.0001 0.0005 0.0003 0.0003 0.0005 0.0003 0.0003 0.0001 0.0001
Nucleus 0.0289 0.0026 0.0057 0.0052 0.0024 0.0025 0.0028 0.0029 0.0043 0.0012 0.0064 0.0032 0.0022 0.0044 0.0031 0.0041 0.0077 0.0023
Peroxisomes 0.0157 0.0055 0.0032 0.0059 0.0215 0.0013 0.0009 0.0006 0.001 0.0002 0.0013 0.0006 0.0005 0.0002 0.0002 0.0004 0.0004 0.0002
Punctate Nuclear 0.0057 0.0018 0.0075 0.0046 0.0084 0.0042 0.0027 0.0014 0.0052 0.0003 0.0052 0.0033 0.0019 0.0017 0.0013 0.0069 0.0022 0.0009
Vacuole 0.0068 0.0033 0.0035 0.0062 0.0038 0.002 0.0031 0.0041 0.0045 0.0014 0.0038 0.002 0.0029 0.005 0.0028 0.0038 0.0024 0.0013
Vacuole Periphery 0.0027 0.0003 0.0005 0.0016 0.0017 0.0004 0.0003 0.0004 0.0007 0.0003 0.0006 0.0004 0.0002 0.0004 0.0003 0.0005 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.0737 11.227 1.1901 5.0774 2.3262 5.3795 3.6487 6.5671 8.8556 5.1509
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
504 1389 1021 2139 2870 2302 1576 1595 3374 3691 2597 3734

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 645.70 813.72 844.33 805.68 757.16 842.37 796.69 889.02 740.51 831.59 815.42 841.28
Standard Deviation 92.01 108.21 105.47 112.05 89.91 101.33 97.41 118.32 98.59 104.90 103.31 121.95
Intensity Change Log 2 0.333668 0.386943 0.319343 0.153856 0.073420 0.231618 0.239410 0.226222 0.272659

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001491 0.001805 0.000556 0.001262 0.000966 0.001316 0.000597 0.001891 0.001045 0.001500 0.000581 0.001530
Bud Neck 0.024860 0.017104 0.005869 0.014179 0.021291 0.034664 0.005151 0.010356 0.021824 0.028056 0.005433 0.012546
Bud Site 0.027575 0.033609 0.014384 0.030209 0.014105 0.031337 0.008885 0.041219 0.016117 0.032192 0.011047 0.034912
Cell Periphery 0.005244 0.000685 0.000572 0.000279 0.000704 0.000692 0.000506 0.000701 0.001382 0.000690 0.000532 0.000459
Cytoplasm 0.270746* 0.380001* 0.465315* 0.553018* 0.419678* 0.390422* 0.545445* 0.519968* 0.397431* 0.386500* 0.513942* 0.538901*
Cytoplasmic Foci 0.210613* 0.262783* 0.050262 0.055889 0.212715* 0.241981* 0.074148 0.058271 0.212401* 0.249809* 0.064758 0.056906
Eisosomes 0.002330 0.000460 0.000138 0.000124 0.001487 0.001159 0.000194 0.000231 0.001613 0.000896 0.000172 0.000170
Endoplasmic Reticulum 0.120409* 0.048787 0.010984 0.001940 0.054271 0.026141 0.009636 0.004616 0.064151 0.034663 0.010166 0.003083
Endosome 0.023178 0.027482 0.004445 0.006600 0.013826 0.028339 0.004390 0.005050 0.015223 0.028017 0.004411 0.005938
Golgi 0.043704 0.017570 0.000184 0.001539 0.026724 0.019170 0.000334 0.003398 0.029261 0.018568 0.000275 0.002333
Lipid Particles 0.056110 0.021871 0.005149 0.003124 0.040359 0.028848 0.005049 0.003211 0.042712 0.026222 0.005088 0.003161
Mitochondria 0.118547* 0.011216 0.000338 0.002768 0.014283 0.014811 0.000324 0.002441 0.029858 0.013458 0.000329 0.002628
Mitotic Spindle 0.000277 0.001290 0.000528 0.023285 0.000331 0.002573 0.000371 0.007985 0.000323 0.002090 0.000433 0.016749
None 0.002326 0.002311 0.002103 0.002102 0.005991 0.002761 0.001966 0.003013 0.005444 0.002592 0.002020 0.002491
Nuclear Periphery 0.000804 0.000773 0.001711 0.001016 0.000477 0.000682 0.000705 0.000632 0.000526 0.000716 0.001100 0.000852
Nuclear Periphery Foci 0.001533 0.003615 0.006369 0.001296 0.001059 0.001076 0.006197 0.002632 0.001129 0.002032 0.006264 0.001867
Nucleolus 0.001179 0.000711 0.000383 0.000879 0.000917 0.001008 0.000293 0.000190 0.000956 0.000896 0.000328 0.000584
Nucleus 0.033161 0.066264 0.326927* 0.130168* 0.117202 0.076392 0.220182* 0.155002* 0.104648 0.072581 0.262148* 0.140776*
Peroxisomes 0.017511 0.006224 0.000500 0.001638 0.005861 0.008772 0.000765 0.001218 0.007601 0.007813 0.000661 0.001458
Vacuole 0.024202 0.088811 0.103098 0.166003 0.045061 0.083426 0.114650 0.177367 0.041945 0.085453 0.110108 0.170857
Vacuole Periphery 0.014200 0.006627 0.000186 0.002684 0.002692 0.004428 0.000213 0.000611 0.004411 0.005255 0.000202 0.001799

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.12 4.82 1.09 1.70 -0.91 -2.02 3.64 -2.48 -1.08 -3.94 -3.09 5.06 -0.81 0.79 -2.45
Bud Neck 2.67 6.71 4.41 3.12 -5.23 -6.52 12.71 8.51 13.46 -5.08 -3.96 14.13 8.74 12.87 -7.67
Bud Site -1.78 3.83 0.94 3.16 -3.41 -8.39 3.93 -5.91 -0.17 -7.72 -9.68 3.67 -6.07 2.10 -8.55
Cell Periphery 6.23 6.39 6.86 9.52 9.63 0.29 6.04 1.90 1.58 -1.15 5.90 7.35 8.51 7.72 4.40
Cytoplasm -9.76 -14.34 -21.83 -14.16 -6.54 4.56 -15.14 -5.79 -8.80 6.84 1.99 -15.87 -15.62 -16.96 0.64
Cytoplasmic Foci -5.64 20.27 20.44 36.38 -0.72 -5.71 34.29 41.47 41.66 6.50 -8.53 42.63 48.63 55.67 5.20
Eisosomes 8.10 9.59 9.73 8.85 2.84 2.72 19.67 19.40 9.07 -0.91 7.75 22.01 22.37 11.08 1.97
Endoplasmic Reticulum 7.14 11.34 12.41 14.94 10.24 9.38 17.29 20.21 12.19 5.94 9.78 20.44 23.89 19.48 11.63
Endosome -1.82 9.52 8.89 15.11 -2.03 -12.23 17.40 14.57 20.65 0.73 -12.90 19.86 17.23 25.42 -1.30
Golgi 7.33 12.75 12.40 14.85 -2.56 5.89 29.48 21.03 14.19 -2.56 9.27 31.44 27.71 21.24 -3.49
Lipid Particles 8.22 12.72 13.34 15.37 5.45 6.84 28.55 30.68 21.53 5.95 11.20 31.08 33.23 26.36 8.08
Mitochondria 12.77 14.16 13.92 8.36 -3.91 -0.44 19.45 14.85 12.51 -3.27 9.79 19.34 17.84 14.93 -5.09
Mitotic Spindle -3.21 -2.17 -8.49 -7.92 -8.35 -3.37 -0.39 -4.55 -2.72 -4.52 -4.10 -1.26 -9.58 -7.94 -9.50
None 0.15 0.68 1.03 0.92 0.16 4.18 6.33 4.47 0.56 -0.78 4.69 6.25 5.73 1.15 -0.43
Nuclear Periphery 0.15 -7.29 -3.25 -3.18 5.11 -2.24 -6.53 -7.66 -1.80 -2.24 -2.47 -13.79 -10.10 -4.20 1.23
Nuclear Periphery Foci -3.52 -7.98 0.50 4.18 8.95 -0.20 -11.30 -9.69 -8.99 7.59 -4.23 -14.61 -5.70 0.39 12.02
Nucleolus 1.06 1.47 0.96 -0.30 -1.95 -0.64 10.77 12.87 7.60 3.77 0.89 5.27 2.97 2.89 -1.78
Nucleus -6.94 -34.59 -20.21 -16.22 23.01 12.66 -19.78 -9.04 -17.77 9.55 11.70 -33.23 -12.39 -23.59 23.18
Peroxisomes 8.01 12.27 11.56 15.26 -7.03 -5.81 22.38 18.90 16.60 -1.76 -0.47 23.80 20.95 20.84 -6.12
Vacuole -15.86 -17.41 -33.69 -21.44 -13.46 -14.26 -19.96 -31.22 -23.92 -15.55 -19.75 -25.39 -45.31 -32.50 -20.46
Vacuole Periphery 4.94 10.09 8.28 5.59 -3.46 -6.16 18.36 13.39 14.82 -1.97 -2.18 17.08 8.08 9.48 -3.72
Standard name
Human Ortholog
Description Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (35%), cytoplasm (14%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Far3

Far3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Far3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available