Standard name | |
---|---|
Human Ortholog | |
Description | UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 6 |
Bud | 0 | 0 | 0 | 3 | 0 | 4 | 4 | 6 | 5 | 5 | 2 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 5 | 9 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 6 | 3 | 5 | 1 | 0 | 8 | 10 | 4 | 14 | 6 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 294 | 264 | 224 | 166 | 162 | 136 | 183 | 132 | 99 | 137 | 94 | 87 | 585 | 701 | 704 | 395 | 394 | 297 | 296 | 241 | 229 | 398 | 440 | 544 |
Endoplasmic Reticulum | 4 | 4 | 3 | 0 | 0 | 1 | 0 | 0 | 6 | 5 | 7 | 1 | 2 | 5 | 5 | 13 | 14 | 7 | 1 | 2 | 6 | 3 | 4 | 8 |
Endosome | 0 | 0 | 2 | 5 | 5 | 1 | 4 | 3 | 1 | 2 | 1 | 3 | 0 | 1 | 2 | 1 | 6 | 1 | 5 | 5 | 4 | 5 | 15 | 33 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 6 |
Mitochondria | 10 | 5 | 0 | 20 | 14 | 74 | 162 | 135 | 133 | 181 | 176 | 139 | 0 | 0 | 1 | 3 | 11 | 3 | 3 | 1 | 1 | 3 | 4 | 8 |
Nucleus | 6 | 3 | 4 | 3 | 0 | 12 | 35 | 31 | 32 | 53 | 20 | 23 | 0 | 2 | 6 | 19 | 7 | 12 | 25 | 27 | 25 | 65 | 37 | 42 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 3 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 3 |
Nucleolus | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 3 | 8 | 7 |
Vac/Vac Membrane | 20 | 20 | 72 | 71 | 47 | 58 | 99 | 109 | 60 | 61 | 49 | 69 | 0 | 18 | 25 | 99 | 106 | 54 | 9 | 12 | 10 | 16 | 28 | 29 |
Unique Cell Count | 322 | 283 | 280 | 231 | 207 | 232 | 378 | 339 | 290 | 360 | 279 | 257 | 588 | 715 | 726 | 485 | 481 | 348 | 359 | 301 | 294 | 515 | 568 | 717 |
Labelled Cell Count | 336 | 297 | 308 | 268 | 230 | 287 | 492 | 430 | 342 | 451 | 351 | 328 | 597 | 738 | 750 | 547 | 544 | 381 | 359 | 301 | 294 | 515 | 568 | 717 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 7.0 | 7.5 | 6.8 | 6.9 | 6.2 | 5.8 | 5.8 | 5.4 | 5.3 | 4.9 | 5.0 | 8.8 | 8.8 | 8.6 | 9.5 | 8.4 | 8.9 | 8.8 | 9.4 | 10.3 |
Std Deviation (1e-4) | 0.8 | 1.3 | 1.3 | 1.4 | 1.2 | 1.3 | 1.4 | 1.5 | 1.1 | 1.2 | 1.1 | 1.1 | 1.1 | 1.2 | 1.2 | 1.9 | 1.9 | 1.8 | 1.3 | 1.5 | 4.4 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.11 | -0.28 | -0.36 | -0.37 | -0.47 | -0.5 | -0.61 | -0.57 | 0.24 | 0.24 | 0.2 | 0.35 | 0.16 | 0.25 | 0.23 | 0.32 | 0.46 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 |
Cytoplasm | -2.2 | -0.5 | -5.3 | -8.2 | -10.3 | -11.0 | -10.6 | -11.1 | -10.8 | 10.7 | 10.0 | 9.0 | 0.5 | 0.7 | 1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.7 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.0 | 4.4 | 10.2 | 12.6 | 11.9 | 12.9 | 14.0 | 16.1 | 14.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 2.4 | 4.2 | 4.1 | 4.7 | 5.9 | 3.3 | 4.0 | 0 | 0 | 0 | 1.9 | 0 | 1.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.3 | -0.8 | -0.2 | 0.1 | 1.8 | -1.4 | -2.7 | -2.3 | 0.3 | -12.8 | -11.5 | -10.7 | -1.7 | -1.2 | -3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.3014 | 5.9411 | 5.7186 | 5.527 | 5.166 | 5.7986 | 3.7694 | 4.2974 | 4.2523 | 3.7943 | 4.2621 | 4.1475 | 3.7263 | 4.461 | 3.7451 | 3.4405 | 3.3962 | 3.9638 |
Actin | 0.0071 | 0.003 | 0.0103 | 0.0003 | 0.0458 | 0.0089 | 0.1018 | 0.0022 | 0.0025 | 0.0177 | 0.0041 | 0.013 | 0.042 | 0.0045 | 0.0137 | 0.0115 | 0.048 | 0.0049 |
Bud | 0.0017 | 0.0011 | 0.0045 | 0.0002 | 0.0005 | 0.001 | 0.0022 | 0.0005 | 0.0005 | 0.0021 | 0.0008 | 0.0011 | 0.0079 | 0.0154 | 0.0102 | 0.0037 | 0.0002 | 0.0057 |
Bud Neck | 0.003 | 0.0009 | 0.0039 | 0.0007 | 0.0044 | 0.0033 | 0.0282 | 0.0006 | 0.0021 | 0.0064 | 0.0015 | 0.0058 | 0.0015 | 0.0006 | 0.0012 | 0.0011 | 0.0006 | 0.002 |
Bud Periphery | 0.0032 | 0.0006 | 0.0024 | 0.0001 | 0.0006 | 0.0004 | 0.0021 | 0.0004 | 0.0006 | 0.0026 | 0.0004 | 0.0013 | 0.0031 | 0.0033 | 0.0034 | 0.0026 | 0.0001 | 0.0041 |
Bud Site | 0.0012 | 0.0024 | 0.0044 | 0.0004 | 0.0012 | 0.0004 | 0.0149 | 0.0057 | 0.0141 | 0.0192 | 0.0013 | 0.002 | 0.0042 | 0.0148 | 0.0074 | 0.0016 | 0.0002 | 0.0002 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0008 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 |
Cytoplasm | 0.7286 | 0.6973 | 0.6087 | 0.7986 | 0.7125 | 0.6573 | 0.4264 | 0.8832 | 0.8199 | 0.6244 | 0.7725 | 0.674 | 0.6836 | 0.7955 | 0.7577 | 0.7947 | 0.8915 | 0.7299 |
Cytoplasmic Foci | 0.0124 | 0.0078 | 0.0109 | 0.0093 | 0.0155 | 0.0163 | 0.0801 | 0.0107 | 0.0025 | 0.0482 | 0.034 | 0.0345 | 0.0293 | 0.0194 | 0.0093 | 0.0164 | 0.0062 | 0.0071 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0109 | 0.0054 | 0.0069 | 0.0028 | 0.0061 | 0.007 | 0.0149 | 0.0031 | 0.0027 | 0.005 | 0.0059 | 0.0102 | 0.0112 | 0.0029 | 0.0042 | 0.0044 | 0.0014 | 0.0041 |
Endosome | 0.0192 | 0.0061 | 0.0125 | 0.0052 | 0.0295 | 0.0168 | 0.0868 | 0.0051 | 0.0028 | 0.0666 | 0.053 | 0.056 | 0.0172 | 0.002 | 0.0062 | 0.0369 | 0.0029 | 0.0056 |
Golgi | 0.0019 | 0.0005 | 0.0024 | 0.0002 | 0.0077 | 0.0015 | 0.0269 | 0.0045 | 0.0007 | 0.0487 | 0.016 | 0.0226 | 0.0056 | 0.0004 | 0.0015 | 0.0016 | 0.0029 | 0.0026 |
Lipid Particles | 0.0014 | 0.0001 | 0.0009 | 0 | 0.0077 | 0.001 | 0.0248 | 0.0008 | 0.0006 | 0.0306 | 0.0133 | 0.007 | 0.0108 | 0.0002 | 0.0052 | 0.0008 | 0.0025 | 0.0005 |
Mitochondria | 0.0021 | 0.0013 | 0.0029 | 0.0006 | 0.0062 | 0.0011 | 0.0074 | 0.0008 | 0.0008 | 0.035 | 0.0033 | 0.0057 | 0.003 | 0.0005 | 0.0031 | 0.0049 | 0.0016 | 0.0008 |
None | 0.0574 | 0.0322 | 0.0427 | 0.0911 | 0.0263 | 0.0457 | 0.0102 | 0.0104 | 0.0107 | 0.0075 | 0.0053 | 0.0063 | 0.03 | 0.0161 | 0.0284 | 0.0201 | 0.0039 | 0.0067 |
Nuclear Periphery | 0.0174 | 0.0144 | 0.0133 | 0.005 | 0.0152 | 0.0158 | 0.028 | 0.0064 | 0.006 | 0.004 | 0.0099 | 0.0161 | 0.0159 | 0.0067 | 0.0099 | 0.0199 | 0.002 | 0.0198 |
Nucleolus | 0.001 | 0.0009 | 0.0011 | 0.0002 | 0.001 | 0.0008 | 0.0025 | 0.0001 | 0.0004 | 0.0018 | 0.0003 | 0.0019 | 0.0006 | 0.0003 | 0.0005 | 0.0012 | 0 | 0.0004 |
Nucleus | 0.1139 | 0.2087 | 0.2503 | 0.0794 | 0.0942 | 0.2034 | 0.0725 | 0.0578 | 0.1276 | 0.0151 | 0.0622 | 0.1027 | 0.0989 | 0.1042 | 0.1213 | 0.0622 | 0.0247 | 0.1883 |
Peroxisomes | 0.0032 | 0.0009 | 0.002 | 0.0003 | 0.0101 | 0.0015 | 0.0197 | 0.0027 | 0.0003 | 0.0435 | 0.0034 | 0.012 | 0.0101 | 0.001 | 0.0031 | 0.001 | 0.0086 | 0.0017 |
Punctate Nuclear | 0.0089 | 0.0138 | 0.017 | 0.0042 | 0.0116 | 0.015 | 0.0388 | 0.0027 | 0.0017 | 0.0031 | 0.0077 | 0.0167 | 0.0217 | 0.011 | 0.0111 | 0.0086 | 0.0017 | 0.0128 |
Vacuole | 0.0042 | 0.0022 | 0.0022 | 0.0012 | 0.002 | 0.002 | 0.0078 | 0.002 | 0.0022 | 0.0145 | 0.0039 | 0.0087 | 0.0021 | 0.001 | 0.0017 | 0.0048 | 0.0007 | 0.0019 |
Vacuole Periphery | 0.001 | 0.0005 | 0.0006 | 0.0003 | 0.0011 | 0.0007 | 0.0024 | 0.0002 | 0.0003 | 0.0031 | 0.0011 | 0.0023 | 0.0009 | 0.0002 | 0.0004 | 0.0019 | 0.0002 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.1684 | 24.4426 | 21.6479 | 28.6854 | 17.1536 | 25.5644 | 29.8958 | 30.401 | 28.789 | 24.5248 |
Translational Efficiency | 1.7516 | 1.3998 | 1.5167 | 1.1381 | 1.5027 | 1.7386 | 1.1898 | 1.221 | 1.2745 | 1.2872 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
224 | 132 | 2250 | 1510 | 124 | 1768 | 211 | 92 | 348 | 1900 | 2461 | 1602 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 872.08 | 973.24 | 902.50 | 1028.91 | 860.22 | 844.22 | 1130.62 | 1105.93 | 867.85 | 853.18 | 922.06 | 1033.33 |
Standard Deviation | 118.82 | 137.53 | 145.04 | 150.88 | 111.88 | 117.24 | 134.81 | 147.49 | 116.53 | 123.21 | 157.71 | 151.75 |
Intensity Change Log 2 | — | 0.158335 | 0.049466 | 0.238584 | — | -0.027087 | 0.394337 | 0.362482 | — | 0.069235 | 0.231007 | 0.301439 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000292 | 0.000580 | 0.000249 | 0.000988 | 0.001251 | 0.000361 | 0.000784 | 0.000967 | 0.000634 | 0.000376 | 0.000295 | 0.000987 |
Bud Neck | 0.003185 | 0.003226 | 0.009308 | 0.003919 | 0.001894 | 0.005266 | 0.002781 | 0.001616 | 0.002725 | 0.005124 | 0.008749 | 0.003787 |
Bud Site | 0.006502 | 0.010102 | 0.012404 | 0.022031 | 0.012884 | 0.014092 | 0.005602 | 0.006953 | 0.008776 | 0.013815 | 0.011821 | 0.021165 |
Cell Periphery | 0.000259 | 0.000224 | 0.000726 | 0.000176 | 0.000671 | 0.000128 | 0.000359 | 0.000192 | 0.000406 | 0.000134 | 0.000694 | 0.000177 |
Cytoplasm | 0.466855* | 0.571346* | 0.614581* | 0.566025* | 0.549449* | 0.665323* | 0.483784* | 0.417760* | 0.496285* | 0.658794* | 0.603367* | 0.557510* |
Cytoplasmic Foci | 0.094583 | 0.141815 | 0.029223 | 0.017853 | 0.106613 | 0.103319 | 0.034027 | 0.060967 | 0.098869 | 0.105994 | 0.029635 | 0.020329 |
Eisosomes | 0.000065 | 0.000059 | 0.000602 | 0.000030 | 0.000101 | 0.000045 | 0.000090 | 0.000090 | 0.000078 | 0.000046 | 0.000558 | 0.000033 |
Endoplasmic Reticulum | 0.004142 | 0.004086 | 0.000974 | 0.003677 | 0.008502 | 0.001232 | 0.007599 | 0.004792 | 0.005696 | 0.001430 | 0.001542 | 0.003741 |
Endosome | 0.005479 | 0.010966 | 0.005644 | 0.003448 | 0.008510 | 0.007941 | 0.009169 | 0.010719 | 0.006559 | 0.008151 | 0.005946 | 0.003866 |
Golgi | 0.001367 | 0.000834 | 0.000960 | 0.002490 | 0.001004 | 0.002137 | 0.000332 | 0.000389 | 0.001237 | 0.002047 | 0.000906 | 0.002369 |
Lipid Particles | 0.005115 | 0.003045 | 0.001856 | 0.001630 | 0.005212 | 0.002822 | 0.004455 | 0.004293 | 0.005150 | 0.002837 | 0.002079 | 0.001783 |
Mitochondria | 0.002153 | 0.000178 | 0.002186 | 0.002584 | 0.000823 | 0.003983 | 0.003914 | 0.000112 | 0.001679 | 0.003719 | 0.002334 | 0.002442 |
Mitotic Spindle | 0.004246 | 0.002015 | 0.014733 | 0.046612 | 0.000476 | 0.017039 | 0.001082 | 0.001287 | 0.002903 | 0.015995 | 0.013562 | 0.044009 |
None | 0.002245 | 0.002995 | 0.012703 | 0.007066 | 0.004371 | 0.004798 | 0.002506 | 0.001398 | 0.003003 | 0.004673 | 0.011828 | 0.006741 |
Nuclear Periphery | 0.001553 | 0.000642 | 0.000929 | 0.001185 | 0.000617 | 0.000407 | 0.001527 | 0.001099 | 0.001220 | 0.000423 | 0.000981 | 0.001180 |
Nuclear Periphery Foci | 0.002581 | 0.006505 | 0.001225 | 0.005328 | 0.009877 | 0.001766 | 0.016037 | 0.011604 | 0.005180 | 0.002095 | 0.002495 | 0.005689 |
Nucleolus | 0.000825 | 0.000397 | 0.000917 | 0.000341 | 0.001099 | 0.000502 | 0.000480 | 0.000174 | 0.000923 | 0.000495 | 0.000880 | 0.000332 |
Nucleus | 0.340362* | 0.149188* | 0.200595* | 0.175687* | 0.237538* | 0.108025 | 0.224233* | 0.119916 | 0.303723* | 0.110885 | 0.202621* | 0.172484* |
Peroxisomes | 0.001258 | 0.001355 | 0.001647 | 0.000305 | 0.001310 | 0.002458 | 0.002535 | 0.001290 | 0.001276 | 0.002381 | 0.001723 | 0.000361 |
Vacuole | 0.056088 | 0.089971 | 0.087320 | 0.136409 | 0.046611 | 0.056867 | 0.198301 | 0.354117* | 0.052711 | 0.059167 | 0.096836 | 0.148912 |
Vacuole Periphery | 0.000845 | 0.000472 | 0.001218 | 0.002213 | 0.001187 | 0.001489 | 0.000402 | 0.000265 | 0.000967 | 0.001418 | 0.001148 | 0.002102 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.00 | 1.23 | -4.93 | -1.76 | -6.82 | 3.81 | 1.72 | 0.23 | -1.85 | -0.73 | 2.59 | 3.75 | -1.55 | -4.92 | -6.55 |
Bud Neck | -0.07 | -5.57 | -2.83 | -2.14 | 4.08 | -5.48 | -1.26 | -1.34 | 3.40 | -0.15 | -4.03 | -6.44 | -4.42 | 0.78 | 3.96 |
Bud Site | -0.72 | -0.96 | -2.06 | -1.54 | -1.98 | -0.24 | 1.54 | 1.10 | 3.19 | -1.50 | -1.45 | -0.30 | -1.62 | -0.20 | -2.14 |
Cell Periphery | 0.78 | -2.20 | 2.11 | 2.26 | 4.18 | 8.07 | 4.67 | 6.79 | -2.82 | 3.48 | 6.25 | -0.73 | 5.70 | -2.50 | 4.34 |
Cytoplasm | -3.79 | -6.54 | 0.43 | 5.09 | 14.72 | -4.85 | 4.36 | 6.22 | 11.68 | 2.89 | -10.46 | -5.18 | 3.03 | 23.03 | 14.44 |
Cytoplasmic Foci | -2.64 | 8.17 | 9.33 | 8.63 | 6.57 | 0.29 | 7.42 | 6.24 | 9.30 | 0.00 | -1.08 | 11.21 | 12.67 | 27.28 | 6.43 |
Eisosomes | 1.05 | -3.72 | 5.52 | 3.57 | 4.12 | 6.16 | 1.03 | 1.47 | -2.58 | 0.77 | 6.22 | -3.60 | 8.17 | 2.66 | 4.15 |
Endoplasmic Reticulum | 0.40 | 4.97 | 1.34 | 0.77 | -11.56 | 6.59 | 0.55 | 2.41 | -4.83 | 2.18 | 7.57 | 6.99 | 3.61 | -10.39 | -8.97 |
Endosome | -1.54 | 1.51 | 4.09 | 2.84 | 3.54 | 0.58 | 0.33 | 1.43 | 1.31 | 1.34 | -1.28 | 2.49 | 5.84 | 10.03 | 3.98 |
Golgi | 0.49 | 0.60 | 0.42 | -0.12 | -0.32 | -2.04 | 3.56 | 3.76 | 3.72 | 0.43 | -1.00 | 0.81 | 0.50 | 1.90 | -0.34 |
Lipid Particles | 1.27 | 2.44 | 2.01 | 1.48 | -1.82 | 3.64 | 1.76 | 1.25 | -1.01 | 0.09 | 2.35 | 3.49 | 2.93 | 1.15 | -1.58 |
Mitochondria | 1.46 | 0.23 | 0.55 | -2.93 | 0.84 | -3.57 | -0.67 | 2.86 | 4.33 | 0.98 | -1.76 | -0.17 | 0.40 | 2.76 | 1.03 |
Mitotic Spindle | 0.75 | -3.46 | -7.57 | -8.99 | -5.50 | -6.71 | -0.51 | -2.87 | 3.81 | -2.74 | -4.87 | -4.60 | -8.56 | -4.51 | -5.69 |
None | -0.91 | -5.80 | -5.25 | -3.08 | 3.19 | -0.34 | 2.74 | 1.05 | 1.65 | -1.56 | -2.55 | -5.00 | -3.55 | -0.82 | 3.04 |
Nuclear Periphery | 2.97 | 0.53 | -1.99 | -10.89 | -5.93 | 2.54 | -5.87 | -3.29 | -4.02 | -0.94 | 4.34 | -0.91 | -4.36 | -17.86 | -6.04 |
Nuclear Periphery Foci | -2.35 | 1.77 | -1.23 | 1.99 | -8.31 | 2.42 | -1.38 | -0.39 | -4.47 | 1.32 | 2.50 | 2.22 | 0.32 | -6.92 | -6.03 |
Nucleolus | 2.41 | 0.43 | 2.58 | 0.38 | 3.03 | 3.36 | 4.06 | 2.13 | 0.27 | -0.23 | 3.14 | 1.11 | 4.00 | 2.32 | 2.98 |
Nucleus | 7.78 | 6.91 | 6.61 | -3.71 | -0.61 | 6.22 | 0.41 | 2.79 | -2.43 | 2.79 | 13.01 | 6.38 | 6.29 | -15.34 | 0.09 |
Peroxisomes | -0.11 | 0.10 | 2.83 | 4.36 | 4.42 | -2.82 | -0.09 | 0.88 | 2.58 | 0.49 | -2.63 | 0.14 | 4.14 | 6.20 | 4.29 |
Vacuole | -2.22 | -5.89 | -15.69 | -10.48 | -15.26 | -2.12 | -12.25 | -11.59 | -11.32 | -4.60 | -0.51 | -10.45 | -21.30 | -26.64 | -15.20 |
Vacuole Periphery | 0.94 | -0.10 | 0.13 | -0.78 | 0.25 | -0.54 | 3.15 | 3.70 | 3.13 | 1.62 | -0.92 | 0.37 | 0.51 | 1.26 | 0.23 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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