Standard name
Human Ortholog
Description N alpha-acetyl-transferase; involved in acetylation of the N-terminal residues of histones H4 and H2A
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.58 0.49 0.62 0.6 0.62 0.51 0.53 0.48 0.24 0.24 0.21 0.21 0.81 0.83 0.88 0.4 0.31 0.33 0.42 0.39 0.46 0.53 0.7 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.16 0 0 0.23 0.23 0.47 0.16 0.66 0.51 0.6 0.51 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.17 0.15 0.12 0.22 0.1 0.22 0.17 0.29 0.31 0.35 0.36 0.44 0 0 0 0.31 0.37 0.32 0.34 0.35 0.26 0.16 0.08 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Vac/Vac Membrane 0.39 0.36 0.35 0.15 0.12 0.15 0 0.18 0.05 0.05 0.07 0.09 0.19 0.23 0.21 0.4 0.42 0.5 0.13 0.15 0.13 0.11 0.08 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 0 2 0 1 2
Bud 0 1 0 1 5 6 9 2 6 5 14 5 0 0 0 1 0 0 4 3 11 9 14 18
Bud Neck 0 0 3 0 9 1 11 3 2 5 2 4 0 0 0 0 0 0 2 2 5 2 4 5
Bud Site 0 0 0 0 0 0 1 2 6 5 4 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 2 2 0 0 1 1 0 0 2 2 0 0 0 0 0 2 1 1
Cytoplasm 132 136 270 122 151 235 210 273 90 85 92 90 150 226 268 83 54 43 110 135 249 97 245 221
Endoplasmic Reticulum 2 1 0 1 0 0 0 0 0 0 0 0 0 1 0 14 10 3 0 0 1 4 1 2
Endosome 0 5 14 7 3 11 0 11 1 2 3 2 8 3 9 5 0 2 5 3 4 1 1 4
Golgi 1 0 2 2 0 0 0 0 0 0 0 0 1 0 0 2 0 1 2 4 2 0 0 0
Mitochondria 12 44 9 8 57 105 188 89 244 178 260 220 0 0 1 12 3 5 1 9 12 2 2 1
Nucleus 39 42 53 45 25 103 68 162 113 120 157 191 5 2 2 66 64 41 88 122 139 29 27 8
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 1 1 0 2 6 13 0 6 0 6 0 0 0 0 0 0 8 9 27 6 11 11
Vac/Vac Membrane 89 100 155 31 29 69 4 100 20 19 32 39 35 63 63 84 73 64 33 53 67 19 28 14
Unique Cell Count 226 278 439 205 244 465 400 568 370 347 434 433 185 271 306 210 172 129 263 353 541 182 349 302
Labelled Cell Count 276 330 508 219 279 532 499 657 484 425 565 567 199 295 346 270 204 159 263 353 541 182 349 302


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.2 6.0 5.5 4.5 4.6 3.4 5.6 3.8 4.2 4.1 4.2 5.6 5.4 6.1 7.0 8.1 9.1 13.9 11.4 10.5
Std Deviation (1e-4) 0.7 0.7 1.4 2.0 1.7 1.3 1.1 2.7 1.0 0.9 0.9 1.0 1.2 1.1 6.4 1.7 1.5 1.7 21.4 19.7 16.9
Intensity Change (Log2) – – – -0.11 -0.43 -0.39 -0.81 -0.1 -0.68 -0.52 -0.55 -0.5 -0.11 -0.14 0.01 0.22 0.44 0.61 1.21 0.93 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.2 1.6 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6243 2.9829 2.7664 2.3829 2.9499 2.8165 3.979 4.328 4.1491 4.3194 4.0566 4.1048 2.481 2.7408 2.3107 2.1967 2.3185 2.2108
Actin 0.0315 0.0004 0.0161 0.0091 0.0022 0.0004 0.013 0.0001 0.004 0.0002 0.0003 0.0007 0.0287 0.0002 0.0029 0.0045 0.0028 0.0003
Bud 0.0007 0.0028 0.0006 0.0005 0.0001 0.0002 0.0007 0.0001 0.0067 0 0.0001 0.0001 0.0006 0.0002 0.0003 0.0002 0.0002 0.0001
Bud Neck 0.0066 0.0008 0.0006 0.0175 0.0019 0.0041 0.0008 0.0003 0.0012 0.0002 0.0013 0.0019 0.0024 0.0004 0.0005 0.0007 0.0006 0.0028
Bud Periphery 0.0007 0.0006 0.001 0.0004 0.0003 0.0001 0.0006 0 0.0018 0 0 0 0.0012 0.0002 0.0003 0.0002 0.0002 0.0001
Bud Site 0.0109 0.0095 0.0015 0.0137 0.0006 0.0003 0.0057 0.0006 0.0012 0.0001 0.0002 0.0002 0.0041 0.0006 0.0006 0.0002 0.0004 0.0002
Cell Periphery 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0.0011 0 0 0 0 0
Cytoplasm 0.2547 0.3212 0.1926 0.1465 0.2133 0.2443 0.1796 0.2629 0.1537 0.0669 0.2399 0.2212 0.1923 0.2311 0.1607 0.2037 0.2001 0.2535
Cytoplasmic Foci 0.0299 0.0049 0.0054 0.01 0.0185 0.0021 0.0482 0.0011 0.0088 0.0035 0.003 0.0098 0.0159 0.0018 0.0056 0.0142 0.0139 0.0056
Eisosomes 0.0004 0 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0 0 0.0016 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0052 0.0046 0.0031 0.0022 0.005 0.0031 0.0072 0.0027 0.0047 0.001 0.0042 0.0022 0.0111 0.0022 0.0021 0.006 0.0027 0.0023
Endosome 0.0194 0.0024 0.0085 0.0124 0.0229 0.002 0.0329 0.0013 0.0048 0.001 0.0048 0.0046 0.0169 0.0014 0.0037 0.003 0.0086 0.0036
Golgi 0.0089 0.0002 0.0072 0.0072 0.009 0 0.0089 0 0.0028 0.0001 0.0001 0.0003 0.006 0 0.0005 0.0005 0.0068 0.0001
Lipid Particles 0.0215 0.0004 0.0065 0.0079 0.0333 0.0001 0.0135 0.0001 0.0072 0.0006 0.0002 0.0009 0.0148 0.0001 0.0004 0.0022 0.0076 0.0002
Mitochondria 0.0067 0.0004 0.0051 0.0165 0.0456 0.0005 0.0059 0.0003 0.0149 0.0002 0.0006 0.0004 0.0056 0.0005 0.003 0.0007 0.0036 0.0006
None 0.156 0.1926 0.1621 0.1203 0.1779 0.1391 0.1384 0.2546 0.1675 0.2339 0.2213 0.2024 0.1936 0.2299 0.2077 0.1669 0.2201 0.1521
Nuclear Periphery 0.0104 0.0084 0.0138 0.0088 0.0131 0.0068 0.018 0.0091 0.0131 0.0081 0.0108 0.0076 0.016 0.0079 0.0104 0.0119 0.0095 0.0083
Nucleolus 0.004 0.0023 0.0028 0.0025 0.0012 0.003 0.0032 0.0009 0.0017 0.0019 0.0026 0.0017 0.0028 0.0015 0.0019 0.0015 0.0023 0.0027
Nucleus 0.3735 0.412 0.5568 0.5949 0.4368 0.5705 0.4516 0.458 0.5823 0.6605 0.4944 0.5058 0.4313 0.5063 0.5663 0.548 0.4746 0.5371
Peroxisomes 0.015 0.0003 0.0056 0.0051 0.0101 0.0001 0.0395 0 0.0082 0.0001 0.0001 0.0082 0.0151 0.0001 0.0019 0.0106 0.0258 0.0002
Punctate Nuclear 0.0404 0.0334 0.0081 0.0223 0.0042 0.0213 0.0263 0.0055 0.0135 0.0212 0.014 0.0309 0.0346 0.014 0.0298 0.0237 0.0189 0.0285
Vacuole 0.0029 0.0024 0.0017 0.0014 0.0024 0.0016 0.0046 0.0021 0.0013 0.0003 0.0017 0.0009 0.0034 0.0015 0.001 0.0009 0.001 0.0015
Vacuole Periphery 0.0007 0.0002 0.0007 0.0006 0.0011 0.0002 0.0011 0.0002 0.0004 0.0001 0.0004 0.0002 0.0011 0.0002 0.0003 0.0002 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.2882 10.3466 5.9715 4.6793 5.0021 3.8559 4.6495 6.6685 4.2999 9.572
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1295 895 175 2223 1847 2459 2190 1951 3142 3354 2365 4174

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.08 723.18 955.42 983.58 713.95 856.69 950.73 1031.67 677.73 821.06 951.08 1006.06
Standard Deviation 114.85 109.85 105.40 141.43 92.84 103.90 128.48 142.28 111.24 120.92 126.92 143.84
Intensity Change Log 2 — 0.208008 0.609788 0.651695 — 0.262950 0.413213 0.531087 — 0.237541 0.508396 0.588694

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000076 0.000499 0.001059 0.000502 0.000051 0.000313 0.000330 0.000555 0.000061 0.000363 0.000384 0.000527
Bud Neck 0.010164 0.015688 0.003319 0.022500 0.008609 0.013919 0.004971 0.009072 0.009250 0.014391 0.004849 0.016223
Bud Site 0.001741 0.008193 0.003646 0.025920 0.003355 0.008419 0.002152 0.007307 0.002690 0.008358 0.002263 0.017220
Cell Periphery 0.000241 0.000221 0.000144 0.000164 0.000132 0.000151 0.000107 0.000149 0.000177 0.000170 0.000109 0.000157
Cytoplasm 0.337865* 0.350326* 0.045712 0.258422* 0.301052* 0.350528* 0.083691 0.194812* 0.316225* 0.350474* 0.080881 0.228689*
Cytoplasmic Foci 0.134245 0.108101 0.001358 0.003347 0.016463 0.043407 0.000998 0.002412 0.065007 0.060671 0.001025 0.002910
Eisosomes 0.000201 0.000084 0.000058 0.000024 0.000018 0.000038 0.000016 0.000021 0.000093 0.000050 0.000019 0.000022
Endoplasmic Reticulum 0.001097 0.002928 0.012755 0.003327 0.001130 0.001240 0.004036 0.004554 0.001117 0.001691 0.004682 0.003900
Endosome 0.004153 0.008604 0.001538 0.005601 0.001541 0.004171 0.000846 0.001735 0.002617 0.005354 0.000897 0.003794
Golgi 0.001406 0.001959 0.000086 0.001732 0.000716 0.001357 0.000258 0.001866 0.001000 0.001518 0.000245 0.001795
Lipid Particles 0.012396 0.005784 0.001202 0.000674 0.001140 0.003036 0.000247 0.000477 0.005780 0.003769 0.000318 0.000582
Mitochondria 0.005164 0.009364 0.000764 0.005383 0.002544 0.006687 0.001009 0.001843 0.003624 0.007401 0.000991 0.003729
Mitotic Spindle 0.000892 0.005237 0.000596 0.086247 0.003686 0.010687 0.004360 0.017872 0.002534 0.009233 0.004081 0.054287
None 0.025187 0.011719 0.002429 0.009171 0.007202 0.005507 0.007030 0.008384 0.014614 0.007165 0.006689 0.008803
Nuclear Periphery 0.001122 0.000969 0.001543 0.003189 0.001227 0.001491 0.001535 0.002104 0.001184 0.001351 0.001536 0.002682
Nuclear Periphery Foci 0.000618 0.001018 0.000705 0.002212 0.000161 0.000275 0.000302 0.000587 0.000349 0.000473 0.000332 0.001452
Nucleolus 0.003289 0.004176 0.001024 0.000888 0.001423 0.002106 0.000414 0.000325 0.002192 0.002658 0.000459 0.000625
Nucleus 0.437735* 0.422481* 0.892568* 0.520634* 0.640119* 0.520585* 0.879463* 0.721569* 0.556705* 0.494407* 0.880432* 0.614555*
Peroxisomes 0.005140 0.007945 0.000228 0.000409 0.000980 0.003260 0.000044 0.000383 0.002695 0.004510 0.000058 0.000397
Vacuole 0.016826 0.033453 0.029090 0.045291 0.008055 0.021464 0.008041 0.023651 0.011670 0.024663 0.009599 0.035176
Vacuole Periphery 0.000441 0.001253 0.000178 0.004366 0.000396 0.001356 0.000150 0.000321 0.000414 0.001328 0.000152 0.002475

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.86 -9.30 -14.24 0.32 6.04 -3.52 -20.83 -9.68 -3.43 -2.83 -5.43 -21.96 -15.61 -3.06 -1.18
Bud Neck -2.68 3.75 -2.53 0.88 -9.38 -3.51 1.53 -1.62 2.70 -5.27 -4.21 2.75 -3.29 1.87 -8.98
Bud Site -7.15 -1.61 -7.42 -1.87 -2.03 -4.39 1.32 -2.43 2.43 -4.32 -6.81 0.19 -7.03 -0.17 -7.17
Cell Periphery 0.95 3.60 8.85 5.28 2.82 -1.26 3.12 -0.10 1.35 -4.79 0.49 8.35 5.88 3.87 -3.71
Cytoplasm -0.47 29.62 18.21 15.73 -17.31 -5.77 33.62 21.25 30.62 -14.78 -4.75 46.13 26.92 32.96 -24.60
Cytoplasmic Foci 3.64 26.45 26.48 21.41 -0.43 -12.15 13.95 13.28 21.89 -2.02 1.30 26.74 26.49 29.71 -2.85
Eisosomes 2.44 2.70 3.66 12.60 5.75 -10.63 -3.72 -10.24 2.93 -7.35 2.16 3.53 3.18 9.92 -5.26
Endoplasmic Reticulum -7.26 -10.75 -16.12 -4.58 8.27 -0.47 -14.28 -14.63 -18.65 -1.41 -3.38 -20.05 -19.65 -19.31 4.63
Endosome -4.04 4.81 5.07 6.38 0.44 -4.87 2.15 1.37 6.78 -1.21 -5.61 5.92 3.96 8.60 -2.27
Golgi -0.74 5.91 3.79 2.50 -2.17 -1.43 1.74 0.32 1.58 -1.01 -1.39 3.88 1.85 2.96 -1.56
Lipid Particles 5.52 10.13 10.89 10.31 3.89 -7.25 6.91 3.67 9.80 -8.37 3.85 11.72 11.07 13.96 -8.17
Mitochondria -2.09 5.80 2.69 3.47 -6.70 -4.86 3.06 1.82 6.64 -4.21 -4.59 6.18 2.77 6.74 -7.38
Mitotic Spindle -3.09 -1.03 -11.53 -9.54 -9.43 -3.97 -1.02 -5.06 -1.25 -4.27 -5.10 -2.09 -12.15 -7.57 -10.18
None 4.77 8.69 5.91 1.66 -13.83 2.05 2.01 -0.68 -3.23 -3.35 5.95 7.49 5.08 -2.58 -6.39
Nuclear Periphery 0.62 -3.49 -12.82 -19.58 -8.89 -1.91 -8.78 -15.48 -12.79 -9.79 -1.48 -9.12 -19.81 -19.92 -14.55
Nuclear Periphery Foci -2.47 -1.57 -0.76 1.18 0.55 -2.36 -4.27 -5.53 -4.66 -2.93 -1.93 -1.61 -3.57 -2.60 -2.63
Nucleolus -1.40 6.88 8.01 7.38 1.59 -2.43 5.19 6.21 6.06 0.75 -1.63 9.36 9.78 8.72 -0.57
Nucleus 0.90 -26.88 -20.66 -19.24 14.23 12.76 -30.37 -12.35 -27.84 20.14 7.88 -48.41 -20.07 -29.95 33.43
Peroxisomes -2.64 8.52 8.71 9.10 2.70 -4.60 3.12 2.58 7.92 -2.19 -3.76 8.84 8.44 11.92 -3.08
Vacuole -7.57 -4.41 -17.89 -8.95 -2.58 -9.31 -6.34 -17.15 -11.12 -13.08 -10.95 -5.70 -25.24 -16.44 -19.02
Vacuole Periphery -5.26 5.33 -1.91 0.61 -2.71 -4.08 3.94 3.45 5.25 -1.02 -5.17 5.57 -1.21 3.01 -3.06
Standard name
Human Ortholog
Description N alpha-acetyl-transferase; involved in acetylation of the N-terminal residues of histones H4 and H2A
Localization
Cell Percentages nucleus (32%), cytoplasm (5%), mixed (56%)
Cell Cycle Regulation Yes
cytoplasm - MA (4.2e-05)
Subcompartmental Group N/A

Nat4

Nat4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nat4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available