Standard name | |
---|---|
Human Ortholog | |
Description | Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments; may have a role in intracellular sterol transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 35 | 55 | 38 | 65 | 84 | 143 | 105 | 117 | – | 144 | 125 | 170 | – | 45 | 24 | 5 | 14 | 21 | 7 | 11 | 11 | 0 | 4 | 5 |
Bud | 6 | 0 | 9 | 13 | 25 | 53 | 46 | 34 | – | 44 | 59 | 80 | – | 0 | 3 | 0 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | – | 3 | 6 | 6 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 3 | 1 | 0 | 3 | 1 | 3 | 3 | – | 5 | 6 | 9 | – | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 16 | 5 | 17 | 27 | 25 | 27 | 35 | – | 27 | 33 | 36 | – | 5 | 6 | 2 | 5 | 4 | 1 | 3 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 16 | 23 | 9 | 17 | 46 | 74 | 57 | 62 | – | 87 | 30 | 71 | – | 18 | 16 | 2 | 3 | 7 | 2 | 2 | 0 | 0 | 2 | 8 |
Endosome | 6 | 1 | 9 | 34 | 42 | 29 | 49 | 47 | – | 73 | 49 | 37 | – | 2 | 1 | 0 | 0 | 1 | 36 | 40 | 25 | 2 | 4 | 12 |
Golgi | 177 | 188 | 112 | 168 | 279 | 205 | 149 | 283 | – | 304 | 109 | 206 | – | 184 | 180 | 30 | 56 | 69 | 175 | 172 | 123 | 16 | 29 | 77 |
Mitochondria | 40 | 26 | 94 | 144 | 237 | 395 | 418 | 259 | – | 327 | 326 | 477 | – | 22 | 14 | 6 | 11 | 5 | 2 | 5 | 1 | 0 | 2 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | – | 3 | 2 | 0 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | – | 3 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 10 | 4 | 4 | 4 | 16 | 17 | 53 | 87 | – | 32 | 13 | 40 | – | 3 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 2 | 6 | 1 | 3 | 5 | – | 3 | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 3 | 4 | 1 | 2 | 12 | – | 12 | 9 | 6 | – | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 32 | 45 | 4 | 13 | 26 | 21 | 35 | 40 | – | 41 | 31 | 36 | – | 21 | 13 | 8 | 4 | 12 | 6 | 21 | 3 | 0 | 2 | 4 |
Unique Cell Count | 220 | 245 | 159 | 284 | 498 | 606 | 667 | 711 | 736 | 543 | 774 | 205 | 199 | 40 | 68 | 84 | 237 | 265 | 170 | 24 | 48 | 118 | ||
Labelled Cell Count | 327 | 361 | 286 | 480 | 796 | 970 | 949 | 991 | 1108 | 800 | 1177 | 300 | 259 | 55 | 97 | 126 | 237 | 265 | 170 | 24 | 48 | 118 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 18.2 | 21.6 | 12.9 | 12.9 | 11.9 | 11.2 | 10.9 | 11.9 | – | 11.4 | 11.1 | 11.5 | – | 20.3 | 20.4 | 24.8 | 27.7 | 27.5 | – | 20.6 | 21.0 |
Std Deviation (1e-4) | 3.5 | 3.5 | 1.9 | 2.1 | 1.9 | 1.8 | 1.8 | 2.4 | – | 2.4 | 2.3 | 2.5 | – | 4.4 | 4.8 | 7.8 | 7.7 | 7.3 | – | 3.8 | 4.0 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.12 | -0.21 | -0.25 | -0.12 | – | -0.18 | -0.22 | -0.17 | – | 0.65 | 0.66 | 0.94 | 1.1 | 1.09 | – | 0.67 | 0.7 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.2 | -2.0 | -0.1 | -2.4 | -2.2 | – | -1.2 | -0.2 | -0.5 | – | -0.4 | -2.9 | -1.6 | -0.5 | 0.2 |
Bud | -0.5 | -0.3 | 1.3 | 0.6 | -0.5 | – | 0.2 | 2.0 | 1.8 | – | 0 | -2.2 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | 1.2 | 0.6 | 0.5 | 1.0 | – | 0.3 | 1.4 | 0.8 | – | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.1 | 1.4 | 2.4 | 1.2 | 1.3 | – | 2.3 | -0.1 | 1.4 | – | 1.1 | 0.9 | 0 | 0 | 0.8 |
Endosome | 2.2 | 1.1 | -0.5 | 0.7 | 0.4 | – | 1.7 | 1.4 | -0.5 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.4 | -3.2 | -8.3 | -11.7 | -7.0 | – | -6.7 | -12.0 | -10.6 | – | 4.7 | 4.9 | 0.6 | 1.9 | 2.0 |
Mitochondria | -1.7 | -2.5 | 1.4 | 0.8 | -5.3 | – | -3.4 | 0.2 | 0.6 | – | -9.8 | -10.7 | -5.0 | -5.9 | -8.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.4 | 3.6 | – | 1.1 | 0 | 1.4 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.1 | 1.4 | 0.6 | 1.5 | 1.6 | – | 1.6 | 1.6 | 1.2 | – | 2.9 | 1.8 | 0 | 0 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.7137 | 15.7868 | 15.227 | 14.1487 | 13.8695 | 15.8306 | 10.5128 | 14.9753 | 13.3803 | 11.9341 | 12.8518 | 13.9872 | 14.3351 | 15.9359 | 14.71 | 12.7302 | 14.237 | 14.1709 |
Actin | 0.0063 | 0.0089 | 0.0088 | 0.0104 | 0.0026 | 0.0089 | 0.0448 | 0.0312 | 0.0279 | 0.0244 | 0.0086 | 0.0183 | 0.0357 | 0.0135 | 0.0172 | 0.0441 | 0.0149 | 0.0086 |
Bud | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0003 |
Bud Periphery | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0008 | 0.0002 | 0.0003 | 0.0002 | 0.0014 | 0.0001 | 0.0011 | 0.0008 | 0.0003 | 0.0001 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0013 | 0.003 | 0.001 | 0.0014 | 0.0006 | 0.0012 | 0.0035 | 0.0024 | 0.0015 | 0.0014 | 0.0007 | 0.0017 | 0.002 | 0.0034 | 0.0019 | 0.0008 | 0.0008 | 0.0009 |
Cytoplasmic Foci | 0.0433 | 0.0728 | 0.0398 | 0.0376 | 0.028 | 0.0342 | 0.0657 | 0.0667 | 0.0496 | 0.0483 | 0.039 | 0.0245 | 0.0522 | 0.0633 | 0.0399 | 0.0387 | 0.03 | 0.0323 |
Eisosomes | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0006 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0022 | 0.0005 | 0.0005 | 0.0008 | 0.0005 | 0.0005 | 0.0006 | 0.0005 | 0.0005 | 0.0003 | 0.0003 | 0.0005 |
Endosome | 0.1622 | 0.2009 | 0.1522 | 0.1356 | 0.1083 | 0.0968 | 0.1999 | 0.1459 | 0.1914 | 0.2962 | 0.2352 | 0.0946 | 0.1607 | 0.1845 | 0.1832 | 0.2052 | 0.1774 | 0.1008 |
Golgi | 0.7546 | 0.6887 | 0.7847 | 0.7785 | 0.8422 | 0.846 | 0.5772 | 0.7088 | 0.6932 | 0.5753 | 0.6673 | 0.8542 | 0.7115 | 0.7184 | 0.7412 | 0.6957 | 0.7569 | 0.8383 |
Lipid Particles | 0.0104 | 0.0078 | 0.0029 | 0.0234 | 0.0057 | 0.0043 | 0.0397 | 0.0097 | 0.0124 | 0.0365 | 0.0086 | 0.001 | 0.019 | 0.0061 | 0.0042 | 0.004 | 0.0024 | 0.0092 |
Mitochondria | 0.0123 | 0.0061 | 0.0046 | 0.0048 | 0.0087 | 0.0033 | 0.0166 | 0.0037 | 0.0096 | 0.0045 | 0.0082 | 0.002 | 0.0053 | 0.0035 | 0.0031 | 0.0019 | 0.0096 | 0.0058 |
None | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0.0003 | 0.0015 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0115 | 0.0001 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleolus | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0037 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0019 | 0.0014 | 0.001 | 0.0033 | 0.0002 | 0.0015 | 0.0208 | 0.0013 | 0.0079 | 0.002 | 0.0215 | 0.0004 | 0.0032 | 0.0016 | 0.001 | 0.0046 | 0.0002 | 0.0009 |
Punctate Nuclear | 0.0002 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0033 | 0 | 0.001 | 0.0002 | 0.0007 | 0.0001 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0025 | 0.0056 | 0.0014 | 0.001 | 0.0006 | 0.0009 | 0.0033 | 0.0193 | 0.0011 | 0.0028 | 0.0032 | 0.0007 | 0.0038 | 0.0016 | 0.0027 | 0.0014 | 0.0028 | 0.0007 |
Vacuole Periphery | 0.0029 | 0.0032 | 0.0026 | 0.0012 | 0.0023 | 0.0012 | 0.0034 | 0.0094 | 0.002 | 0.0058 | 0.0034 | 0.001 | 0.0023 | 0.0018 | 0.0036 | 0.0025 | 0.0041 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.5555 | 31.3135 | 43.5675 | 52.3192 | 34.0622 | 17.5047 | 41.5552 | 43.406 | 54.7267 | 51.4461 |
Translational Efficiency | 2.7104 | 2.5187 | 1.9028 | 1.6057 | 2.0859 | 5.6338 | 2.9297 | 2.0156 | 1.9158 | 1.925 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
857 | 1484 | 2718 | 1097 | 2035 | 1742 | 1186 | 1414 | 2892 | 3226 | 3904 | 2511 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1194.72 | 1540.55 | 2036.32 | 2223.31 | 1544.46 | 1459.94 | 2000.21 | 2086.45 | 1440.82 | 1497.02 | 2025.35 | 2146.24 |
Standard Deviation | 210.87 | 296.02 | 321.48 | 436.18 | 268.95 | 253.37 | 292.58 | 411.18 | 299.30 | 276.74 | 313.42 | 427.71 |
Intensity Change Log 2 | — | 0.366773 | 0.769292 | 0.896037 | — | -0.081193 | 0.373049 | 0.433948 | — | 0.131454 | 0.559372 | 0.653863 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.010984 | 0.008598 | 0.015413 | 0.084392 | 0.004582 | 0.006390 | 0.025730 | 0.062966 | 0.006479 | 0.007406 | 0.018547 | 0.072327 |
Bud Neck | 0.000999 | 0.000253 | 0.000689 | 0.010338 | 0.000153 | 0.000285 | 0.001215 | 0.005977 | 0.000404 | 0.000270 | 0.000849 | 0.007883 |
Bud Site | 0.005042 | 0.001886 | 0.001134 | 0.041310 | 0.000967 | 0.001654 | 0.002773 | 0.026952 | 0.002175 | 0.001760 | 0.001632 | 0.033224 |
Cell Periphery | 0.000258 | 0.000135 | 0.000292 | 0.096546 | 0.000112 | 0.000112 | 0.000598 | 0.046179 | 0.000155 | 0.000122 | 0.000385 | 0.068184 |
Cytoplasm | 0.000909 | 0.000803 | 0.000186 | 0.001927 | 0.000256 | 0.000739 | 0.000236 | 0.002135 | 0.000449 | 0.000768 | 0.000202 | 0.002044 |
Cytoplasmic Foci | 0.112880 | 0.085634 | 0.030762 | 0.015885 | 0.055635 | 0.102386 | 0.022979 | 0.023646 | 0.072598 | 0.094680 | 0.028397 | 0.020255 |
Eisosomes | 0.000107 | 0.000082 | 0.000257 | 0.013308 | 0.000074 | 0.000069 | 0.000303 | 0.004808 | 0.000084 | 0.000075 | 0.000271 | 0.008522 |
Endoplasmic Reticulum | 0.001805 | 0.001021 | 0.003675 | 0.040615 | 0.000963 | 0.000946 | 0.004820 | 0.031552 | 0.001212 | 0.000980 | 0.004023 | 0.035511 |
Endosome | 0.107152 | 0.073325 | 0.069640 | 0.073275 | 0.063296 | 0.105411 | 0.055425 | 0.101224 | 0.076292 | 0.090651 | 0.065322 | 0.089014 |
Golgi | 0.689940* | 0.780707* | 0.769775* | 0.486509* | 0.827544* | 0.725614* | 0.803809* | 0.564714* | 0.786767* | 0.750957* | 0.780114* | 0.530548* |
Lipid Particles | 0.016220 | 0.018598 | 0.072516 | 0.085664 | 0.008962 | 0.019230 | 0.052580 | 0.091736 | 0.011113 | 0.018939 | 0.066460 | 0.089083 |
Mitochondria | 0.018532 | 0.006055 | 0.005286 | 0.004544 | 0.010637 | 0.009201 | 0.002299 | 0.004168 | 0.012977 | 0.007754 | 0.004379 | 0.004333 |
Mitotic Spindle | 0.001433 | 0.001414 | 0.000371 | 0.001272 | 0.000197 | 0.000926 | 0.000495 | 0.000802 | 0.000563 | 0.001150 | 0.000409 | 0.001007 |
None | 0.000083 | 0.000089 | 0.000070 | 0.000351 | 0.000044 | 0.000031 | 0.000071 | 0.000165 | 0.000056 | 0.000058 | 0.000070 | 0.000246 |
Nuclear Periphery | 0.000129 | 0.000113 | 0.000410 | 0.000684 | 0.000079 | 0.000130 | 0.000289 | 0.000306 | 0.000094 | 0.000122 | 0.000373 | 0.000471 |
Nuclear Periphery Foci | 0.000594 | 0.000444 | 0.000761 | 0.000820 | 0.000212 | 0.000679 | 0.000565 | 0.000791 | 0.000326 | 0.000571 | 0.000702 | 0.000804 |
Nucleolus | 0.000082 | 0.000203 | 0.000009 | 0.000044 | 0.000004 | 0.000010 | 0.000008 | 0.000038 | 0.000027 | 0.000099 | 0.000009 | 0.000040 |
Nucleus | 0.000048 | 0.000430 | 0.000017 | 0.000169 | 0.000007 | 0.000017 | 0.000020 | 0.000196 | 0.000019 | 0.000207 | 0.000018 | 0.000185 |
Peroxisomes | 0.001681 | 0.000681 | 0.000176 | 0.001520 | 0.000237 | 0.000855 | 0.000196 | 0.001337 | 0.000665 | 0.000775 | 0.000182 | 0.001417 |
Vacuole | 0.016000 | 0.009350 | 0.013034 | 0.029061 | 0.009843 | 0.012596 | 0.014160 | 0.019708 | 0.011668 | 0.011103 | 0.013376 | 0.023794 |
Vacuole Periphery | 0.015122 | 0.010179 | 0.015525 | 0.011764 | 0.016197 | 0.012717 | 0.011430 | 0.010598 | 0.015878 | 0.011550 | 0.014281 | 0.011108 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.00 | -1.84 | -12.73 | -13.74 | -12.43 | -1.55 | -7.76 | -14.12 | -13.51 | -7.64 | -0.85 | -8.39 | -19.46 | -19.21 | -15.29 |
Bud Neck | 3.13 | 1.11 | -11.77 | -13.28 | -12.55 | -3.27 | -4.62 | -8.61 | -8.42 | -6.62 | 1.73 | -3.59 | -14.59 | -14.97 | -13.51 |
Bud Site | 3.04 | 4.60 | -12.34 | -13.65 | -14.19 | -2.24 | -4.79 | -11.63 | -11.26 | -10.66 | 0.97 | 1.65 | -17.39 | -17.54 | -17.81 |
Cell Periphery | 4.23 | -1.29 | -14.09 | -14.10 | -14.08 | 0.01 | -4.49 | -10.96 | -10.96 | -10.84 | 2.56 | -6.43 | -17.70 | -17.71 | -17.64 |
Cytoplasm | 0.25 | 4.14 | -2.68 | -2.48 | -5.08 | -2.50 | 0.56 | -3.56 | -2.72 | -3.59 | -1.84 | 4.41 | -4.61 | -3.53 | -5.25 |
Cytoplasmic Foci | 5.85 | 20.23 | 23.81 | 26.24 | 10.91 | -16.47 | 19.25 | 17.92 | 29.49 | -0.50 | -9.26 | 24.99 | 29.37 | 39.18 | 7.88 |
Eisosomes | 1.66 | -6.25 | -6.95 | -6.96 | -6.87 | 0.42 | -7.37 | -5.28 | -5.28 | -5.02 | 0.93 | -10.17 | -8.65 | -8.66 | -8.46 |
Endoplasmic Reticulum | 2.22 | -4.33 | -14.80 | -15.22 | -14.11 | 0.11 | -7.31 | -14.91 | -14.91 | -12.63 | 1.60 | -10.18 | -21.10 | -21.27 | -19.18 |
Endosome | 7.23 | 8.34 | 7.13 | -0.01 | -1.13 | -12.67 | 2.81 | -11.05 | 1.10 | -13.12 | -5.49 | 4.55 | -4.72 | 0.61 | -9.42 |
Golgi | -9.35 | -8.39 | 14.70 | 23.91 | 23.34* | 15.70 | 3.33 | 27.06 | 15.64 | 21.88* | 6.74 | 1.35 | 31.98 | 27.72 | 31.18* |
Lipid Particles | -0.88 | -14.89 | -12.15 | -12.09 | -2.13 | -5.05 | -11.33 | -16.64 | -13.92 | -6.36 | -5.12 | -21.09 | -21.01 | -18.46 | -5.18 |
Mitochondria | 4.63 | 5.11 | 5.39 | 1.23 | 0.76 | 0.83 | 6.34 | 4.37 | 3.41 | -1.91 | 3.75 | 6.94 | 6.82 | 3.49 | 0.06 |
Mitotic Spindle | -0.07 | 2.91 | 0.29 | 0.23 | -3.39 | -2.72 | -0.97 | -4.24 | 0.32 | -0.34 | -1.65 | 0.46 | -2.73 | 0.37 | -2.71 |
None | -0.24 | 0.97 | -1.70 | -1.63 | -1.78 | 3.49 | -4.63 | -4.46 | -4.90 | -3.54 | -0.20 | -2.73 | -2.79 | -2.70 | -2.58 |
Nuclear Periphery | 0.56 | -5.53 | -7.12 | -7.32 | -3.04 | -2.74 | -4.47 | -8.83 | -5.88 | -0.36 | -2.01 | -7.63 | -10.28 | -9.15 | -1.91 |
Nuclear Periphery Foci | 1.06 | -1.32 | -1.89 | -3.67 | -0.43 | -1.81 | -4.78 | -7.88 | -0.67 | -2.72 | -1.68 | -4.98 | -8.11 | -1.79 | -1.47 |
Nucleolus | -0.63 | 1.37 | 0.68 | 0.84 | -3.26 | -2.44 | -4.26 | -2.40 | -1.96 | -2.12 | -0.82 | 1.11 | -0.89 | 0.64 | -3.32 |
Nucleus | -1.29 | 1.55 | -5.42 | 0.89 | -13.29 | -2.37 | -9.35 | -1.97 | -1.91 | -1.90 | -1.39 | 0.05 | -2.52 | -0.44 | -2.53 |
Peroxisomes | 2.17 | 4.34 | 0.37 | -2.06 | -5.00 | -3.58 | 0.83 | -5.03 | -1.76 | -5.30 | -0.58 | 4.44 | -3.76 | -2.70 | -7.32 |
Vacuole | 3.20 | 1.47 | -4.86 | -8.49 | -7.19 | -2.35 | -3.06 | -6.45 | -4.43 | -3.07 | 0.53 | -1.80 | -8.73 | -9.30 | -7.68 |
Vacuole Periphery | 3.12 | -0.27 | 2.03 | -1.46 | 3.46 | 2.62 | 3.74 | 4.29 | 1.86 | 0.79 | 4.28 | 1.59 | 4.65 | 0.50 | 4.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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