Standard name | |
---|---|
Human Ortholog | |
Description | ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 2 | 2 | 4 | 4 | 6 | 1 | – | 4 | 3 | 15 | 37 | 38 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 3 | 9 | – | 7 | 5 | 7 | 14 | 34 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 5 | 5 | 3 | 6 | 12 | 11 | 11 | – | 15 | 15 | 1 | 2 | 3 | 23 | 7 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 87 | 148 | 167 | 172 | 178 | 185 | 281 | 259 | 167 | – | 126 | 116 | 109 | 166 | 139 | 336 | 155 | 187 | 99 | 146 | 172 | 107 | 133 | 165 |
Endoplasmic Reticulum | 4 | 9 | 5 | 8 | 9 | 6 | 33 | 22 | 6 | – | 10 | 12 | 15 | 11 | 4 | 14 | 5 | 11 | 3 | 4 | 10 | 13 | 18 | 51 |
Endosome | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | – | 0 | 0 | 7 | 17 | 38 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 13 | 42 | 25 | 4 | 2 | 3 | 0 | 1 | 0 | 0 | 1 | 2 |
Mitochondria | 0 | 1 | 0 | 26 | 8 | 50 | 69 | 161 | 145 | – | 142 | 137 | 2 | 5 | 25 | 6 | 0 | 3 | 0 | 0 | 1 | 1 | 3 | 1 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | – | 0 | 1 | 1 | 1 | 1 | 4 | 3 | 5 | 0 | 0 | 0 | 2 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 2 | 1 | 5 | 3 | 4 | 11 | 5 | – | 5 | 3 | 2 | 4 | 5 | 8 | 10 | 15 | 0 | 3 | 5 | 2 | 5 | 2 |
Unique Cell Count | 90 | 156 | 176 | 191 | 198 | 224 | 353 | 404 | 274 | 244 | 230 | 153 | 265 | 257 | 359 | 171 | 216 | 107 | 160 | 192 | 130 | 171 | 233 | |
Labelled Cell Count | 92 | 161 | 182 | 215 | 208 | 256 | 404 | 477 | 346 | 313 | 292 | 174 | 308 | 321 | 395 | 183 | 234 | 107 | 160 | 192 | 130 | 171 | 233 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 16.2 | 12.7 | 10.4 | 9.7 | 10.2 | 8.8 | 8.0 | 7.3 | 6.9 | – | 6.6 | 6.4 | 12.8 | 12.5 | 10.6 | 14.8 | 15.5 | 16.1 | 13.5 | 13.0 | 14.4 |
Std Deviation (1e-4) | 2.3 | 2.1 | 1.3 | 1.3 | 1.3 | 1.3 | 1.2 | 1.1 | 1.2 | – | 1.3 | 1.1 | 1.9 | 1.8 | 1.6 | 2.9 | 3.2 | 3.4 | 2.5 | 2.9 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.04 | -0.24 | -0.37 | -0.51 | -0.58 | – | -0.65 | -0.7 | 0.3 | 0.27 | 0.02 | 0.51 | 0.58 | 0.63 | 0.38 | 0.32 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 4.3 | 5.2 | 5.3 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.3 | 4.5 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0.3 | 0 | 0.7 | – | 1.6 | 1.7 | 0 | 0 | 0 | 1.7 | 0.6 | 0.5 |
Cytoplasm | -1.7 | -1.8 | -3.8 | -4.6 | -7.7 | -8.0 | – | -9.5 | -9.7 | -5.8 | -7.7 | -9.2 | -0.6 | -1.5 | -2.8 |
Endoplasmic Reticulum | 0.7 | 0.9 | 0 | 2.7 | 1.4 | 0 | – | 0.7 | 1.2 | 2.6 | 0.7 | 0 | 0.6 | 0 | 1.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.4 | 5.3 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.9 | 5.6 | 4.3 | 0 | 0 | 0 |
Mitochondria | 5.1 | 0 | 6.7 | 6.3 | 9.9 | 11.7 | – | 12.4 | 12.6 | 0 | 0 | 4.3 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.2963 | 12.8828 | 12.4265 | 11.188 | 11.8564 | 12.8528 | 9.7926 | 14.6495 | 13.311 | 12.5983 | 11.8316 | 12.8864 | 10.7791 | 15.1521 | 12.9451 | 13.7431 | 13.8091 | 13.8686 |
Actin | 0.0184 | 0.0011 | 0.0126 | 0.0191 | 0.0051 | 0.0025 | 0.0533 | 0.0007 | 0.0159 | 0.0046 | 0.0061 | 0.0091 | 0.0734 | 0.0017 | 0.028 | 0.0022 | 0.0039 | 0.0023 |
Bud | 0.0005 | 0.0004 | 0.001 | 0.001 | 0.004 | 0.0006 | 0.0021 | 0.0002 | 0.0004 | 0.001 | 0.0003 | 0.0003 | 0.0014 | 0.0004 | 0.0038 | 0.0011 | 0.0006 | 0.0004 |
Bud Neck | 0.0064 | 0.0005 | 0.0009 | 0.0022 | 0.0015 | 0.0029 | 0.002 | 0.0003 | 0.0007 | 0.0009 | 0.0006 | 0.0027 | 0.0099 | 0.0002 | 0.0008 | 0.0007 | 0.0007 | 0.0017 |
Bud Periphery | 0.0005 | 0.0002 | 0.0007 | 0.0006 | 0.0067 | 0.0009 | 0.002 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0.0018 | 0.0001 | 0.0017 | 0.0009 | 0.0001 | 0.0002 |
Bud Site | 0.0071 | 0.0025 | 0.001 | 0.0011 | 0.0005 | 0.0002 | 0.0051 | 0.0021 | 0.0013 | 0.0019 | 0.0002 | 0.0004 | 0.0081 | 0.0029 | 0.0044 | 0.0003 | 0.0002 | 0.0001 |
Cell Periphery | 0.0008 | 0.0009 | 0.0008 | 0.0003 | 0.0005 | 0.0007 | 0.0008 | 0.0007 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0011 | 0.0001 | 0.0006 | 0.0011 | 0 | 0.0002 |
Cytoplasm | 0.5549 | 0.8949 | 0.8046 | 0.7171 | 0.7464 | 0.7531 | 0.5009 | 0.9329 | 0.8625 | 0.7372 | 0.8335 | 0.8355 | 0.5273 | 0.9554 | 0.7499 | 0.7347 | 0.8119 | 0.7949 |
Cytoplasmic Foci | 0.0089 | 0.0066 | 0.0081 | 0.0182 | 0.0126 | 0.0133 | 0.0629 | 0.0053 | 0.0251 | 0.0222 | 0.0213 | 0.0188 | 0.0345 | 0.012 | 0.0241 | 0.0238 | 0.0349 | 0.0251 |
Eisosomes | 0.0006 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0003 | 0.0005 | 0 | 0 |
Endoplasmic Reticulum | 0.2319 | 0.0526 | 0.1055 | 0.0693 | 0.1079 | 0.1325 | 0.071 | 0.0364 | 0.0301 | 0.0349 | 0.0194 | 0.0445 | 0.1309 | 0.0043 | 0.0401 | 0.0362 | 0.0162 | 0.0853 |
Endosome | 0.0453 | 0.0183 | 0.0256 | 0.0761 | 0.0589 | 0.051 | 0.1226 | 0.0093 | 0.0296 | 0.0979 | 0.0377 | 0.0547 | 0.0824 | 0.0127 | 0.0667 | 0.1326 | 0.0939 | 0.0575 |
Golgi | 0.0044 | 0.0024 | 0.0032 | 0.0105 | 0.005 | 0.0059 | 0.0303 | 0.0015 | 0.0059 | 0.0063 | 0.0099 | 0.0091 | 0.0255 | 0.0046 | 0.0161 | 0.0123 | 0.0223 | 0.0121 |
Lipid Particles | 0.0045 | 0.0004 | 0.0006 | 0.0053 | 0.0011 | 0.001 | 0.0211 | 0.0004 | 0.0039 | 0.0032 | 0.0021 | 0.0029 | 0.0189 | 0.0001 | 0.0086 | 0.0004 | 0 | 0.0008 |
Mitochondria | 0.0052 | 0.0012 | 0.006 | 0.0044 | 0.0048 | 0.0062 | 0.0161 | 0.0004 | 0.0022 | 0.0159 | 0.0484 | 0.0048 | 0.0084 | 0.0004 | 0.0237 | 0.0106 | 0.0051 | 0.0063 |
None | 0.0118 | 0.0013 | 0.0028 | 0.0028 | 0.0019 | 0.0009 | 0.006 | 0.0007 | 0.0009 | 0.0072 | 0.0019 | 0.0008 | 0.0072 | 0.0006 | 0.0048 | 0.0012 | 0.0006 | 0.0008 |
Nuclear Periphery | 0.0321 | 0.0033 | 0.0074 | 0.0156 | 0.0138 | 0.0053 | 0.0207 | 0.0022 | 0.0016 | 0.0038 | 0.0038 | 0.0041 | 0.0154 | 0.0007 | 0.0041 | 0.0044 | 0.0015 | 0.0023 |
Nucleolus | 0.0011 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0019 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0041 | 0 | 0.0002 | 0 | 0 | 0 |
Nucleus | 0.0081 | 0.0017 | 0.0024 | 0.0069 | 0.005 | 0.0026 | 0.0156 | 0.0019 | 0.0011 | 0.0024 | 0.0032 | 0.002 | 0.0067 | 0.0008 | 0.0018 | 0.0018 | 0.0011 | 0.0012 |
Peroxisomes | 0.0013 | 0.0001 | 0.0006 | 0.0037 | 0.0004 | 0.0004 | 0.0094 | 0 | 0.0014 | 0.0006 | 0.0024 | 0.0016 | 0.013 | 0.0001 | 0.0057 | 0.0002 | 0.0007 | 0.0036 |
Punctate Nuclear | 0.0063 | 0.0001 | 0.0017 | 0.0045 | 0.0032 | 0.0011 | 0.0314 | 0.0001 | 0.0079 | 0.0004 | 0.005 | 0.0007 | 0.0171 | 0.0001 | 0.001 | 0.0002 | 0.0001 | 0.0008 |
Vacuole | 0.0429 | 0.0101 | 0.011 | 0.0311 | 0.0155 | 0.0139 | 0.0205 | 0.0041 | 0.0071 | 0.0478 | 0.0022 | 0.0048 | 0.0093 | 0.0022 | 0.0099 | 0.0256 | 0.0033 | 0.0027 |
Vacuole Periphery | 0.0071 | 0.0015 | 0.0034 | 0.0097 | 0.005 | 0.0052 | 0.004 | 0.0005 | 0.0012 | 0.0102 | 0.0014 | 0.0024 | 0.003 | 0.0004 | 0.0035 | 0.0092 | 0.0028 | 0.0017 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 67.1654 | 45.3273 | 45.4321 | 54.24 | 55.684 | 59.7536 | 55.8344 | 59.3256 | 49.2829 | 63.6315 |
Translational Efficiency | 0.7227 | 0.8903 | 0.8506 | 0.6103 | 0.5841 | 0.6534 | 0.6668 | 0.479 | 0.5723 | 0.5363 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1557 | 1493 | 553 | 1258 | 1742 | 2275 | 1154 | 67 | 3299 | 3768 | 1707 | 1325 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1037.60 | 1360.78 | 1409.72 | 1658.70 | 1102.79 | 1275.00 | 1585.35 | 1650.18 | 1072.02 | 1308.99 | 1528.45 | 1658.27 |
Standard Deviation | 182.25 | 211.44 | 318.28 | 232.57 | 205.81 | 192.79 | 224.29 | 206.40 | 197.75 | 204.74 | 271.26 | 231.33 |
Intensity Change Log 2 | — | 0.391183 | 0.442158 | 0.676803 | — | 0.209339 | 0.523643 | 0.581465 | — | 0.300356 | 0.484716 | 0.628469 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000316 | 0.000603 | 0.001372 | 0.003759 | 0.000313 | 0.000962 | 0.000712 | 0.001072 | 0.000314 | 0.000820 | 0.000926 | 0.003623 |
Bud Neck | 0.000564 | 0.000459 | 0.003354 | 0.002892 | 0.000475 | 0.000464 | 0.002170 | 0.002926 | 0.000517 | 0.000462 | 0.002554 | 0.002894 |
Bud Site | 0.033178 | 0.038388 | 0.047378 | 0.068829 | 0.023929 | 0.033854 | 0.036975 | 0.067240 | 0.028294 | 0.035651 | 0.040345 | 0.068749 |
Cell Periphery | 0.000324 | 0.000642 | 0.002570 | 0.002786 | 0.000461 | 0.000322 | 0.001184 | 0.004283 | 0.000397 | 0.000449 | 0.001633 | 0.002862 |
Cytoplasm | 0.599235* | 0.621055* | 0.566596* | 0.581413* | 0.580415* | 0.638247* | 0.545850* | 0.383836* | 0.589297* | 0.631435* | 0.552571* | 0.571422* |
Cytoplasmic Foci | 0.249895* | 0.234398* | 0.080169 | 0.115019 | 0.238050* | 0.215431* | 0.103430 | 0.155534 | 0.243640* | 0.222947* | 0.095894 | 0.117068 |
Eisosomes | 0.000015 | 0.000010 | 0.000010 | 0.000013 | 0.000011 | 0.000010 | 0.000007 | 0.000011 | 0.000013 | 0.000010 | 0.000008 | 0.000013 |
Endoplasmic Reticulum | 0.008795 | 0.008675 | 0.012453 | 0.001829 | 0.012126 | 0.006790 | 0.008518 | 0.006722 | 0.010554 | 0.007537 | 0.009792 | 0.002077 |
Endosome | 0.013221 | 0.011603 | 0.025418 | 0.020482 | 0.013939 | 0.010836 | 0.028207 | 0.046110 | 0.013600 | 0.011140 | 0.027304 | 0.021778 |
Golgi | 0.034657 | 0.042766 | 0.057925 | 0.060219 | 0.040631 | 0.040227 | 0.035410 | 0.071807 | 0.037812 | 0.041233 | 0.042704 | 0.060805 |
Lipid Particles | 0.001345 | 0.001812 | 0.013151 | 0.023830 | 0.001755 | 0.001080 | 0.012859 | 0.015674 | 0.001562 | 0.001370 | 0.012953 | 0.023418 |
Mitochondria | 0.002151 | 0.000916 | 0.000108 | 0.000262 | 0.002058 | 0.001926 | 0.000157 | 0.000194 | 0.002102 | 0.001526 | 0.000141 | 0.000258 |
Mitotic Spindle | 0.000550 | 0.001833 | 0.011174 | 0.015132 | 0.001285 | 0.001344 | 0.010573 | 0.010481 | 0.000938 | 0.001538 | 0.010768 | 0.014897 |
None | 0.000487 | 0.000247 | 0.000529 | 0.000119 | 0.001653 | 0.000218 | 0.000206 | 0.000019 | 0.001103 | 0.000230 | 0.000311 | 0.000114 |
Nuclear Periphery | 0.000048 | 0.000024 | 0.000440 | 0.000078 | 0.000154 | 0.000041 | 0.000342 | 0.000067 | 0.000104 | 0.000034 | 0.000374 | 0.000078 |
Nuclear Periphery Foci | 0.000645 | 0.000505 | 0.021957 | 0.003545 | 0.001300 | 0.000446 | 0.006333 | 0.002826 | 0.000991 | 0.000469 | 0.011394 | 0.003508 |
Nucleolus | 0.000084 | 0.000024 | 0.000262 | 0.000085 | 0.000172 | 0.000035 | 0.000078 | 0.000098 | 0.000130 | 0.000031 | 0.000138 | 0.000085 |
Nucleus | 0.001527 | 0.000696 | 0.006027 | 0.002366 | 0.002312 | 0.001027 | 0.002673 | 0.000861 | 0.001942 | 0.000896 | 0.003760 | 0.002290 |
Peroxisomes | 0.000390 | 0.000483 | 0.000284 | 0.002783 | 0.000379 | 0.000828 | 0.000777 | 0.002703 | 0.000384 | 0.000691 | 0.000618 | 0.002779 |
Vacuole | 0.018449 | 0.012655 | 0.109363 | 0.081121 | 0.032844 | 0.022006 | 0.174125 | 0.200993* | 0.026050 | 0.018301 | 0.153145 | 0.087182 |
Vacuole Periphery | 0.034123 | 0.022205 | 0.039460 | 0.013437 | 0.045738 | 0.023905 | 0.029414 | 0.026541 | 0.040256 | 0.023231 | 0.032668 | 0.014099 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.37 | -6.36 | -5.45 | -4.99 | -3.60 | -1.49 | -2.81 | -3.57 | -0.13 | -1.23 | -1.91 | -5.62 | -5.51 | -4.29 | -4.38 |
Bud Neck | 1.75 | -10.45 | -14.24 | -14.66 | 1.00 | 0.12 | -10.61 | -5.81 | -5.93 | -1.49 | 0.96 | -15.02 | -14.85 | -15.54 | -2.06 |
Bud Site | -2.30 | -3.87 | -11.18 | -9.14 | -4.88 | -5.35 | -5.37 | -2.71 | -2.05 | -1.82 | -5.11 | -5.97 | -13.50 | -10.92 | -8.44 |
Cell Periphery | -6.79 | -6.55 | -9.09 | -7.74 | -0.17 | 3.01 | -5.40 | -2.31 | -2.40 | -1.84 | -1.59 | -8.62 | -9.09 | -8.87 | -3.80 |
Cytoplasm | -1.98 | 2.23 | 2.13 | 3.90 | -0.59 | -6.02 | 3.10 | 4.94 | 6.51 | 3.92 | -5.85 | 4.17 | 2.36 | 6.53 | -1.24 |
Cytoplasmic Foci | 1.93 | 24.65 | 18.98 | 17.00 | -6.62 | 3.33 | 21.81 | 4.53 | 3.44 | -2.22 | 3.99 | 31.80 | 22.68 | 19.87 | -4.88 |
Eisosomes | 1.68 | 1.68 | 0.61 | -2.83 | -2.44 | 0.91 | 3.25 | 0.13 | -0.38 | -1.69 | 1.90 | 3.15 | 0.02 | -3.02 | -4.94 |
Endoplasmic Reticulum | 0.08 | -1.65 | 7.22 | 6.41 | 5.41 | 3.89 | 2.55 | 1.30 | 0.12 | 0.44 | 3.08 | 0.79 | 10.90 | 7.49 | 7.48 |
Endosome | 1.66 | -6.63 | -5.89 | -7.65 | 2.55 | 3.73 | -9.74 | -3.96 | -4.40 | -2.01 | 3.91 | -11.95 | -7.08 | -9.70 | 3.88 |
Golgi | -4.48 | -6.19 | -7.01 | -4.36 | 0.05 | 0.20 | 2.94 | -1.66 | -1.69 | -2.10 | -2.53 | -1.96 | -6.42 | -5.25 | -4.73 |
Lipid Particles | -2.69 | -8.75 | -14.37 | -14.06 | -4.99 | 4.32 | -9.28 | -3.50 | -3.68 | -0.58 | 1.68 | -12.46 | -14.57 | -14.71 | -5.98 |
Mitochondria | 7.13 | 13.20 | 9.96 | 4.85 | -1.22 | 0.48 | 8.97 | 7.12 | 7.54 | -0.14 | 3.40 | 14.56 | 10.90 | 8.52 | -0.95 |
Mitotic Spindle | -3.64 | -7.82 | -8.02 | -7.31 | -2.47 | -0.14 | -5.84 | -1.10 | -1.09 | -0.08 | -2.05 | -8.30 | -7.82 | -7.55 | -2.24 |
None | 3.05 | -0.31 | 6.96 | 1.95 | 4.37 | 2.82 | 2.82 | 3.20 | 7.16 | 2.17 | 3.22 | 2.89 | 3.64 | 3.55 | 2.92 |
Nuclear Periphery | 1.56 | -3.85 | -2.05 | -4.44 | 3.49 | 2.10 | -2.19 | 1.57 | -1.24 | 3.52 | 2.35 | -4.07 | 0.65 | -3.27 | 4.66 |
Nuclear Periphery Foci | 1.50 | -9.20 | -9.93 | -10.58 | 7.79 | 3.38 | -10.58 | -3.14 | -4.77 | 5.67 | 3.44 | -12.58 | -8.97 | -10.35 | 8.87 |
Nucleolus | 3.76 | -0.98 | -0.34 | -5.52 | 0.94 | 3.01 | 2.02 | 1.32 | -1.83 | -0.51 | 3.96 | -0.10 | 1.44 | -5.23 | 0.79 |
Nucleus | 4.88 | -2.53 | -1.84 | -2.83 | 1.45 | 2.82 | -1.15 | 2.76 | 0.08 | 4.52 | 4.09 | -2.99 | -1.28 | -2.60 | 0.90 |
Peroxisomes | -2.21 | 2.96 | -7.88 | -7.55 | -8.19 | -2.94 | -2.47 | -3.38 | -2.62 | -2.61 | -3.28 | -2.17 | -8.22 | -6.88 | -6.84 |
Vacuole | 3.77 | -12.09 | -16.52 | -18.22 | 3.11 | 4.73 | -21.18 | -6.47 | -6.86 | -1.23 | 5.36 | -24.75 | -15.74 | -17.94 | 9.97 |
Vacuole Periphery | 5.34 | -1.06 | 10.63 | 6.14 | 6.38 | 9.32 | 6.27 | 3.29 | -0.07 | 0.59 | 10.40 | 3.75 | 16.74 | 7.84 | 8.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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