ORF
Human Ortholog
Description Putative protein of unknown function; similar to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0.05 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.94 0.95 1.0 0.98 0.99 0.94 0.94 – 0.73 0.79 0.65 0.8 1.0 1.0 1.0 0.9 0.95 0.92 0.77 0.87 0.77 0.85 0.85 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0.13 0.08 – 0.44 0.36 0.46 0.35 0 0 0 0 0 0 0.09 0.05 0.12 0.08 0.05 0.05
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.08 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 1 0 0 0 0 0 0 0 3 0 0 1 1 1
Bud 1 0 0 0 0 0 0 – 4 1 9 5 1 0 0 0 0 0 0 1 0 0 2 4
Bud Neck 0 0 0 0 4 0 8 – 5 3 5 2 0 0 0 0 0 0 1 0 0 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 5 7 1 0 0 0 1 1 1
Cytoplasm 151 113 37 46 197 82 158 – 75 106 102 163 134 223 239 147 125 109 135 110 54 137 200 173
Endoplasmic Reticulum 0 2 0 0 0 0 0 – 0 0 0 0 0 0 0 6 6 5 2 1 0 0 0 0
Endosome 0 0 0 0 0 0 1 – 0 0 1 1 0 0 0 1 0 0 4 0 1 1 2 2
Golgi 1 1 0 1 0 1 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 0 2 1
Mitochondria 6 8 0 0 0 11 13 – 45 48 72 72 1 0 0 3 4 1 16 6 8 12 12 9
Nucleus 0 0 0 0 1 2 2 – 4 4 4 1 0 1 0 1 6 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 5 – 0 2 0 0 0 0 0 0 0 0 0 1 1 1 2 1
Vac/Vac Membrane 3 0 0 0 0 0 4 – 1 4 2 3 0 0 0 19 11 9 4 1 0 1 2 1
Unique Cell Count 160 119 37 47 198 87 168 103 134 157 204 134 223 239 164 132 118 175 127 71 161 235 205
Labelled Cell Count 164 124 37 47 204 96 191 135 169 196 247 136 225 239 182 159 128 175 127 71 161 235 205


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.1 3.9 4.4 5.0 3.7 4.4 – 3.7 3.7 3.9 3.7 4.6 4.9 5.3 8.2 7.7 7.8 4.7 4.9 5.0
Std Deviation (1e-4) 0.9 1.1 0.3 1.4 1.6 1.7 1.8 – 1.7 1.5 1.9 1.1 1.1 1.6 1.7 2.3 1.9 2.0 3.9 3.5 3.4
Intensity Change (Log2) – – – 0.18 0.36 -0.05 0.2 – -0.08 -0.05 0.03 -0.06 0.24 0.34 0.45 1.08 1.0 1.01 0.29 0.36 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2447 0.216 -0.0558 0.211 -0.2363 -0.1108 -0.3676 0.7993 0.7039 0.5902 1.2126 0.5975 2.0308 3.2522 2.4612 2.5917 2.5229 2.802
Actin 0.0369 0.0003 0.0187 0.0364 0.0354 0.001 0.0002 0.0035 0.0102 0.0001 0.0031 0.0019 0.0173 0.0013 0.0004 0.0004 0.0004 0.0002
Bud 0.0005 0.0001 0.0006 0.001 0.0005 0.0001 0.0054 0.0074 0.0005 0.0001 0.0003 0.0003 0.0001 0.0015 0.0001 0.0002 0 0
Bud Neck 0.0153 0.0002 0.0005 0.0188 0.0005 0.0015 0 0.0001 0.0022 0.0002 0.001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003
Bud Periphery 0.0007 0.0001 0.0004 0.0013 0.0007 0 0.0026 0.0019 0.0012 0.0001 0.0004 0.0009 0.0001 0.0007 0.0001 0.0001 0 0
Bud Site 0.0046 0.0009 0.0019 0.004 0.0009 0.0001 0.001 0.0025 0.0042 0.0004 0.0036 0.0001 0.0004 0.0083 0.0003 0.0002 0 0
Cell Periphery 0.0005 0.0001 0.0001 0.0002 0.0001 0 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001 0 0
Cytoplasm 0.3476 0.5523 0.4246 0.5235 0.5946 0.6069 0.2451 0.3819 0.3693 0.4068 0.5243 0.4982 0.4197 0.525 0.5216 0.4764 0.5279 0.6132
Cytoplasmic Foci 0.0302 0.0136 0.0118 0.0319 0.0192 0.0159 0.0077 0.0068 0.009 0.0077 0.0188 0.0054 0.0095 0.0032 0.0046 0.0062 0.0024 0.0045
Eisosomes 0.0004 0.0002 0.0004 0.0002 0.0005 0 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0058 0.0037 0.0031 0.0048 0.0036 0.0022 0.0014 0.0013 0.0013 0.0009 0.0005 0.0011 0.0022 0.0008 0.0015 0.003 0.0024 0.0012
Endosome 0.0224 0.0045 0.0044 0.0144 0.0194 0.006 0.0011 0.0014 0.0064 0.031 0.0232 0.0034 0.0024 0.0004 0.0014 0.0017 0.0005 0.0011
Golgi 0.0079 0.0003 0.002 0.0117 0.0288 0.0005 0.0001 0.0005 0.0031 0.0013 0.0431 0.0032 0.0017 0.0001 0.0002 0.0001 0 0.0001
Lipid Particles 0.0085 0.0009 0.0053 0.0136 0.0275 0.0017 0.0006 0.0018 0.0035 0.0051 0.0331 0.0008 0.0012 0 0.0001 0.0001 0 0.0001
Mitochondria 0.0049 0.0002 0.0008 0.0081 0.0078 0.0003 0.0014 0.0005 0.0012 0.0003 0.0754 0.0533 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001
None 0.4731 0.4174 0.4958 0.2885 0.2352 0.3413 0.7298 0.5875 0.5783 0.5341 0.2483 0.4262 0.5405 0.4548 0.4645 0.5064 0.4641 0.3759
Nuclear Periphery 0.013 0.001 0.0061 0.0023 0.0025 0.0071 0.0004 0.0003 0.0006 0.0005 0.0003 0.0007 0.0006 0.0003 0.0005 0.0008 0.0004 0.0003
Nucleolus 0.0013 0.0001 0.0006 0.0004 0.0005 0.0002 0.0003 0.0001 0.0002 0.0005 0.0029 0 0.0001 0.0001 0.0001 0.0001 0 0
Nucleus 0.0084 0.0019 0.0091 0.005 0.002 0.0094 0.0008 0.0006 0.0022 0.0008 0.0013 0.0009 0.0011 0.0016 0.0025 0.0016 0.0009 0.0025
Peroxisomes 0.0096 0.0001 0.0087 0.0254 0.0103 0.0004 0.0003 0.0004 0.0045 0.0007 0.0161 0.0019 0.0009 0.0002 0.0001 0.0002 0 0.0001
Punctate Nuclear 0.0024 0.0008 0.0035 0.0064 0.0085 0.0037 0.0004 0.0004 0.0007 0.0004 0.0013 0.0003 0.0006 0.0005 0.0009 0.001 0.0003 0.0003
Vacuole 0.0047 0.0013 0.0012 0.0018 0.0011 0.0013 0.001 0.0006 0.0009 0.0082 0.002 0.0007 0.0007 0.0005 0.0006 0.0009 0.0001 0.0003
Vacuole Periphery 0.0014 0.0001 0.0003 0.0005 0.0005 0.0004 0.0001 0.0001 0.0002 0.0008 0.0007 0.0005 0.0001 0 0.0001 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.3251 24.5152 16.0512 17.319 15.6865 16.1227 21.8711 27.5549 31.849 20.8406
Translational Efficiency 1.7374 0.8542 1.1079 1.392 1.1102 3.5055 1.5129 1.1727 1.3281 1.3844

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2186 1070 171 1467 1072 1907 1826 1160 3258 2977 1997 2627

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 613.78 684.08 910.92 822.25 661.77 737.03 795.91 854.99 629.57 718.00 805.76 836.71
Standard Deviation 75.84 110.90 124.30 165.22 72.95 90.01 112.91 133.52 78.22 101.27 118.38 152.91
Intensity Change Log 2 — 0.156443 0.569603 0.421855 — 0.155393 0.266275 0.369578 — 0.155899 0.420200 0.394970

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000335 0.000818 0.000707 0.001502 0.000131 0.000312 0.000329 0.002318 0.000268 0.000494 0.000361 0.001862
Bud Neck 0.041387 0.028856 0.002018 0.009837 0.010537 0.046329 0.003558 0.011982 0.031236 0.040049 0.003426 0.010784
Bud Site 0.008053 0.017085 0.010939 0.029975 0.002436 0.021054 0.006384 0.082173 0.006205 0.019627 0.006774 0.053024
Cell Periphery 0.000281 0.000160 0.000241 0.000164 0.000149 0.000187 0.000105 0.000393 0.000238 0.000177 0.000117 0.000265
Cytoplasm 0.364762* 0.392595* 0.227860* 0.468344* 0.464319* 0.480697* 0.441365* 0.463637* 0.397520* 0.449031* 0.423083* 0.466266*
Cytoplasmic Foci 0.316595* 0.261248* 0.012821 0.019684 0.247815* 0.138662 0.008074 0.025807 0.293964* 0.182722* 0.008481 0.022388
Eisosomes 0.000567 0.000146 0.000098 0.000045 0.000169 0.000104 0.000024 0.000065 0.000436 0.000119 0.000030 0.000054
Endoplasmic Reticulum 0.002008 0.001115 0.009713 0.001733 0.000512 0.000618 0.001536 0.002286 0.001516 0.000797 0.002236 0.001977
Endosome 0.013162 0.028550 0.004451 0.007846 0.007969 0.014725 0.001516 0.009006 0.011454 0.019694 0.001767 0.008358
Golgi 0.011353 0.005662 0.000128 0.004159 0.002063 0.002399 0.000201 0.007188 0.008296 0.003572 0.000195 0.005497
Lipid Particles 0.020628 0.009447 0.003310 0.001098 0.010356 0.004228 0.000684 0.001587 0.017248 0.006104 0.000909 0.001314
Mitochondria 0.021584 0.009658 0.000471 0.006868 0.004984 0.011084 0.000615 0.007759 0.016122 0.010572 0.000603 0.007262
Mitotic Spindle 0.000582 0.003847 0.009459 0.059192 0.001598 0.011629 0.004514 0.045812 0.000916 0.008832 0.004937 0.053284
None 0.005410 0.004305 0.003780 0.006007 0.002856 0.003461 0.008832 0.004708 0.004570 0.003765 0.008400 0.005433
Nuclear Periphery 0.000410 0.000651 0.002109 0.001891 0.000942 0.000566 0.001292 0.001513 0.000585 0.000597 0.001362 0.001724
Nuclear Periphery Foci 0.000392 0.000650 0.003789 0.001878 0.000457 0.000190 0.000585 0.002501 0.000413 0.000355 0.000859 0.002153
Nucleolus 0.001427 0.003104 0.000782 0.000609 0.000920 0.002028 0.000397 0.000678 0.001260 0.002415 0.000430 0.000640
Nucleus 0.140531* 0.135399* 0.590112* 0.276942* 0.198420* 0.198203* 0.486429* 0.214276* 0.159578* 0.175630* 0.495307* 0.249271*
Peroxisomes 0.016128 0.017928 0.000407 0.001623 0.007708 0.008672 0.000372 0.002168 0.013358 0.011999 0.000375 0.001863
Vacuole 0.032823 0.076117 0.116541 0.097057 0.034940 0.052729 0.032906 0.108884 0.033520 0.061135 0.040067 0.102279
Vacuole Periphery 0.001581 0.002657 0.000266 0.003544 0.000721 0.002122 0.000282 0.005259 0.001298 0.002315 0.000280 0.004301

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.53 -5.01 -2.07 0.25 0.96 -2.21 -1.50 -3.38 -2.62 -2.59 -1.80 -0.83 -3.74 -1.84 -2.80
Bud Neck 4.16 17.86 15.15 8.71 -6.45 -13.14 5.12 1.96 14.85 -4.29 -3.72 17.41 14.94 17.21 -5.68
Bud Site -4.71 -0.37 -3.95 0.59 -1.03 -10.70 -3.13 -9.28 -4.21 -8.28 -10.08 0.21 -9.59 -2.65 -8.92
Cell Periphery 3.73 0.30 4.91 2.48 5.58 -1.95 4.30 -2.08 -0.84 -3.86 2.60 5.71 2.95 0.72 -3.39
Cytoplasm -2.35 10.36 0.06 2.18 -9.91 -1.70 8.70 8.64 10.94 1.02 -7.24 4.88 4.61 10.95 -0.54
Cytoplasmic Foci 6.70 57.93 59.82 36.42 -0.43 14.46 37.20 35.21 29.01 -7.03 20.51 70.76 68.56 44.62 -7.32
Eisosomes 15.68 15.94 19.60 13.00 6.32 6.52 16.17 11.10 5.28 -8.03 17.16 22.00 20.79 11.93 -8.04
Endoplasmic Reticulum 2.62 -7.62 -2.22 -10.43 7.24 -0.78 -13.08 -10.78 -11.05 -1.07 3.17 -7.25 -5.44 -16.38 3.41
Endosome -6.90 8.51 9.52 11.61 1.86 -5.58 10.89 4.69 8.69 -4.22 -7.04 16.18 10.68 14.41 -4.42
Golgi 5.48 14.91 11.95 4.93 -3.23 -0.57 3.60 -0.20 0.12 -2.14 7.53 15.07 9.00 2.97 -3.30
Lipid Particles 8.75 15.78 19.36 10.49 5.78 6.36 10.52 9.71 7.60 -4.46 13.56 21.90 21.46 13.09 -3.47
Mitochondria 5.87 13.78 11.18 4.18 -3.13 -3.10 2.75 1.08 5.19 -2.21 3.85 13.41 10.17 6.97 -3.67
Mitotic Spindle -3.41 -1.44 -9.41 -8.14 -3.32 -5.42 -2.46 -7.59 -4.43 -6.77 -6.77 -4.01 -12.17 -8.15 -9.95
None 1.20 3.29 -0.31 -2.04 -5.26 -0.95 -6.30 -2.75 -1.80 4.37 1.37 -3.02 -0.91 -3.30 3.00
Nuclear Periphery -1.93 -13.03 -23.56 -15.42 -2.97 0.88 -2.60 -4.43 -15.99 -5.81 -0.12 -9.75 -15.16 -23.56 -9.19
Nuclear Periphery Foci -2.20 -7.22 -3.43 -2.48 5.02 1.67 -2.23 -3.28 -4.48 -2.45 0.43 -6.24 -4.79 -5.38 -1.61
Nucleolus -3.89 4.17 5.12 5.90 0.77 -4.08 5.33 3.56 5.84 -1.62 -4.81 7.77 6.15 8.24 -2.26
Nucleus 0.69 -18.63 -25.87 -23.02 9.31 0.09 -37.13 -8.21 -8.59 24.91 -3.33 -52.18 -25.70 -21.15 26.32
Peroxisomes -1.11 19.01 17.70 11.75 -1.12 -0.62 6.54 6.02 8.90 -2.65 1.33 19.33 18.17 14.34 -2.55
Vacuole -12.06 -9.46 -26.11 -16.38 -1.15 -6.85 -8.41 -25.16 -22.56 -20.68 -13.61 -12.23 -36.23 -28.88 -24.51
Vacuole Periphery -3.37 10.18 0.88 2.87 -2.42 -4.19 2.32 -1.36 0.51 -2.13 -4.45 8.55 -0.37 1.95 -3.18
ORF
Human Ortholog
Description Putative protein of unknown function; similar to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability
Localization
Cell Percentages cytoplasm (17%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YMR090W

YMR090W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr090w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available