Standard name
Human Ortholog
Description Protein of unconfirmed function; involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.09 0.11
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.99 – 0.94 0.92 0.76 0.81 – 0.73 0.69 0.75 0.69 0.96 0.99 0.99 0.97 0.95 1.0 0.87 0.89 0.79 0.88 0.83 0.82
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0 – 0.12 0.1 0.19 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0.1 0.07 0.21 0.16 – 0.2 0.16 0.1 0.16 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 1 0 0 – 0 0 2 0 0 0 0 0 0 0 0 1 0 1 0 0
Bud 0 0 – 0 1 1 0 – 0 1 2 3 0 0 2 0 0 0 9 4 0 8 28 49
Bud Neck 0 0 – 0 0 0 1 – 0 1 0 3 0 0 0 0 0 0 2 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 – 0 0 0 3 – 1 1 1 9 2 1 0 2 0 0 0 0 0 0 0 1
Cytoplasm 172 157 – 29 192 68 158 – 96 86 139 179 254 438 544 142 78 18 153 148 18 177 281 377
Endoplasmic Reticulum 0 0 – 0 3 0 4 – 2 0 2 6 2 3 0 9 3 1 1 0 0 1 5 4
Endosome 0 0 – 0 0 0 0 – 0 1 0 1 0 0 2 0 0 0 2 0 0 1 1 2
Golgi 1 0 – 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Mitochondria 1 0 – 0 0 4 3 – 16 13 35 34 0 0 1 3 3 0 0 0 0 1 1 0
Nucleus 0 0 – 0 1 1 6 – 4 3 5 11 2 1 1 0 1 0 0 3 1 3 2 5
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 0 0 – 0 0 0 0 – 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 – 0 1 0 1 – 0 2 1 6 1 0 1 0 0 0 1 0 0 2 5 8
Vac/Vac Membrane 4 2 – 3 15 19 32 – 26 20 18 42 7 7 6 5 0 0 0 4 1 1 5 4
Unique Cell Count 178 159 31 208 90 196 132 124 186 259 265 444 551 146 82 18 176 167 24 203 339 462
Labelled Cell Count 179 160 32 214 93 208 145 131 205 296 268 450 557 162 85 19 176 167 24 203 339 462


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.7 25.1 – 26.8 19.8 9.8 12.3 – 7.6 8.0 6.9 6.8 30.7 26.5 28.7 35.4 34.1 45.5 22.5 24.7 23.7
Std Deviation (1e-4) 14.2 13.6 – 9.6 7.7 4.3 5.2 – 3.0 2.9 2.6 2.6 13.5 12.8 13.0 16.6 16.5 13.2 10.3 11.9 12.3
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.0764 29.2501 28.3117 22.4256 21.0266 25.3653 30.8562 41.7429 38.5243 31.9172 30.7314 35.2823 25.9397 32.6393 32.6236 27.7794 28.1848 30.5443
Actin 0.0024 0.0009 0.0039 0.0008 0.0037 0.001 0.0031 0.0007 0.0008 0.026 0.0118 0.0017 0.0056 0.0003 0.001 0.0004 0.0008 0.0013
Bud 0.0009 0.0011 0.0057 0.0018 0.0012 0.0038 0.0003 0.0016 0.0044 0.0025 0.0015 0.0014 0.0023 0.0035 0.0035 0.0003 0.0008 0.0027
Bud Neck 0.0002 0.0001 0.0005 0.0004 0.0007 0.0008 0.0003 0.0002 0.0004 0.0007 0.0002 0.0009 0.0002 0.0002 0.0002 0.0003 0.0008 0.0007
Bud Periphery 0.001 0.0002 0.0021 0.0008 0.001 0.0019 0.0001 0.0005 0.0011 0.0022 0.0008 0.0006 0.0014 0.0017 0.001 0.0001 0.0005 0.0036
Bud Site 0.0004 0.0018 0.0021 0.0001 0.0003 0.0003 0.0003 0.0021 0.0006 0.0013 0.0002 0.0002 0.001 0.0044 0.0013 0.0001 0.0001 0.0003
Cell Periphery 0.0002 0 0.0001 0 0.0002 0 0.0001 0.0001 0 0.0001 0.0001 0 0.0002 0.0002 0.0001 0 0.0001 0.0002
Cytoplasm 0.8351 0.9792 0.9155 0.9187 0.8302 0.9138 0.9064 0.9714 0.9618 0.9341 0.8146 0.9353 0.8766 0.9721 0.9541 0.927 0.9023 0.9005
Cytoplasmic Foci 0.0161 0.0005 0.0072 0.0012 0.0176 0.004 0.0086 0.0042 0.0015 0.0018 0.0318 0.0012 0.0077 0.0008 0.0029 0.0017 0.0027 0.0036
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0034 0.0004 0.0008 0.0012 0.0061 0.0014 0.0026 0.0003 0.0004 0.0005 0.0004 0.0004 0.0021 0.0005 0.0015 0.0008 0.0021 0.0023
Endosome 0.0055 0.0001 0.0031 0.0007 0.0147 0.0034 0.007 0.0006 0.0018 0.0004 0.0019 0.0002 0.0034 0.0002 0.0024 0.0005 0.0013 0.0027
Golgi 0.0013 0 0.0012 0 0.0009 0.0005 0.0014 0.0003 0.0006 0.0005 0.0026 0 0.0005 0 0.0002 0 0.0001 0.0007
Lipid Particles 0.0045 0 0.0039 0 0.0069 0.0026 0.0067 0.0005 0.0013 0.0002 0.025 0 0.0016 0 0.0005 0 0.0003 0.0006
Mitochondria 0.0094 0.0001 0.0039 0.0001 0.0098 0.0003 0.0029 0.0004 0.0001 0.0002 0.0477 0.0002 0.0008 0.0001 0.0004 0.0001 0.0015 0.0005
None 0.0214 0.0022 0.007 0.0023 0.0028 0.0116 0.0063 0.0101 0.0034 0.0052 0.0007 0.0222 0.0244 0.0055 0.0081 0.0328 0.0459 0.0144
Nuclear Periphery 0.0126 0.0022 0.0028 0.0096 0.017 0.0062 0.008 0.0005 0.0024 0.0016 0.0027 0.0031 0.0086 0.0011 0.0035 0.0027 0.007 0.0058
Nucleolus 0.0003 0 0.0002 0.0001 0.001 0.0001 0.0011 0.0003 0.0001 0.0001 0.0001 0.0001 0.001 0.0001 0.0002 0 0.0003 0.0003
Nucleus 0.0781 0.0104 0.0285 0.0586 0.0278 0.046 0.0369 0.0043 0.0177 0.02 0.0204 0.0314 0.0541 0.008 0.0156 0.0318 0.0277 0.0515
Peroxisomes 0.0007 0 0.0098 0 0.0371 0.0002 0.0004 0.0003 0.0001 0.0007 0.0306 0.0001 0.0003 0 0.0001 0.0001 0.0002 0.0001
Punctate Nuclear 0.0014 0.0004 0.0008 0.0017 0.0043 0.0009 0.0019 0.0002 0.0011 0.0007 0.0046 0.0006 0.0051 0.0006 0.0013 0.0009 0.001 0.0069
Vacuole 0.0025 0.0002 0.0007 0.0014 0.0127 0.0008 0.0028 0.001 0.0002 0.0011 0.0016 0.0005 0.0022 0.0006 0.0015 0.0003 0.0028 0.0009
Vacuole Periphery 0.0026 0 0.0003 0.0004 0.0042 0.0003 0.0026 0.0002 0.0001 0.0002 0.0006 0.0001 0.0007 0.0001 0.0007 0.0001 0.0016 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.0681 10.2291 3.253 2.7904 1.0597 24.5065 24.7484 17.95 23.6847 19.4673
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2423 742 323 101 1284 2295 1240 1066 3707 3037 1563 1167

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1826.27 2526.85 1096.43 836.07 1648.76 2332.72 802.76 822.16 1764.79 2380.15 863.45 823.36
Standard Deviation 558.70 737.00 543.77 118.69 634.94 661.80 106.21 259.90 592.28 686.03 290.16 250.87
Intensity Change Log 2 — 0.468440 -0.736086 -1.127204 — 0.500632 -1.038341 -1.003890 — 0.483803 -0.871641 -1.067381

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000124 0.000198 0.000642 0.000493 0.000329 0.000133 0.000174 0.000954 0.000195 0.000149 0.000271 0.000915
Bud Neck 0.000769 0.002039 0.003634 0.001986 0.001213 0.000925 0.001992 0.004534 0.000923 0.001198 0.002332 0.004314
Bud Site 0.004682 0.004806 0.015197 0.004158 0.005538 0.003011 0.003726 0.043144 0.004978 0.003450 0.006096 0.039770
Cell Periphery 0.000148 0.000308 0.000312 0.000212 0.000434 0.000103 0.000154 0.000264 0.000247 0.000153 0.000186 0.000260
Cytoplasm 0.784030* 0.646926* 0.279506* 0.472668* 0.703043* 0.719099* 0.393098* 0.469315* 0.755979* 0.701466* 0.369623* 0.469605*
Cytoplasmic Foci 0.006518 0.018150 0.025793 0.044839 0.017121 0.004323 0.015528 0.027251 0.010191 0.007701 0.017649 0.028773
Eisosomes 0.000002 0.000011 0.000094 0.000135 0.000009 0.000002 0.000048 0.000059 0.000005 0.000004 0.000057 0.000065
Endoplasmic Reticulum 0.000181 0.000152 0.004256 0.004001 0.000469 0.000145 0.002107 0.003595 0.000281 0.000146 0.002551 0.003630
Endosome 0.000283 0.001226 0.005654 0.008326 0.001200 0.000532 0.002195 0.007698 0.000600 0.000702 0.002910 0.007752
Golgi 0.000409 0.000978 0.000271 0.000198 0.001282 0.000997 0.000119 0.005113 0.000712 0.000993 0.000150 0.004687
Lipid Particles 0.000213 0.000680 0.004313 0.004092 0.000604 0.000218 0.001475 0.001423 0.000349 0.000331 0.002061 0.001654
Mitochondria 0.000202 0.000549 0.000987 0.000175 0.000369 0.000356 0.000669 0.004230 0.000260 0.000403 0.000735 0.003879
Mitotic Spindle 0.001304 0.005155 0.017342 0.000956 0.002414 0.005752 0.005791 0.033239 0.001689 0.005606 0.008178 0.030445
None 0.021509 0.022410 0.007271 0.004275 0.024163 0.020457 0.009945 0.009604 0.022428 0.020934 0.009392 0.009143
Nuclear Periphery 0.000094 0.000157 0.002145 0.001254 0.000166 0.000129 0.001538 0.001145 0.000119 0.000136 0.001664 0.001155
Nuclear Periphery Foci 0.001050 0.001942 0.002659 0.005620 0.002521 0.000881 0.001026 0.000967 0.001560 0.001140 0.001364 0.001370
Nucleolus 0.000197 0.000185 0.001477 0.000564 0.000426 0.000222 0.000590 0.001098 0.000276 0.000213 0.000773 0.001052
Nucleus 0.173819* 0.287200* 0.529978* 0.226669* 0.229207* 0.238765* 0.504413* 0.281068* 0.193004* 0.250599* 0.509696* 0.276359*
Peroxisomes 0.000048 0.001555 0.000841 0.001122 0.000126 0.000110 0.000204 0.001055 0.000075 0.000463 0.000336 0.001061
Vacuole 0.003456 0.005157 0.097216 0.218061* 0.007520 0.003314 0.054814 0.101024 0.004864 0.003764 0.063577 0.111153
Vacuole Periphery 0.000959 0.000216 0.000411 0.000194 0.001845 0.000524 0.000397 0.003221 0.001266 0.000449 0.000400 0.002959

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.81 -7.93 -2.37 -1.32 2.65 4.09 1.75 -2.75 -5.02 -3.85 1.62 -5.10 -4.49 -4.96 -2.74
Bud Neck -3.93 -8.08 -5.15 0.95 5.41 1.93 -6.01 -10.25 -11.18 -6.96 -2.77 -11.60 -11.77 -10.00 -5.07
Bud Site 0.02 -2.06 0.30 0.26 2.14 3.86 2.21 -6.27 -7.14 -6.82 2.63 -0.18 -6.42 -7.02 -6.20
Cell Periphery -6.87 -6.35 -0.87 5.61 5.10 6.71 6.21 4.97 -4.90 -3.56 3.20 3.13 2.81 -0.40 -0.11
Cytoplasm 16.78 43.02 16.18 10.25 -2.60 -1.74 37.21 30.39 35.22 -3.71 11.94 60.05 43.42 35.50 -5.18
Cytoplasmic Foci -4.84 -6.76 -4.86 -1.52 -1.30 7.34 2.48 -0.80 -10.24 -3.97 1.84 -3.81 -6.60 -7.80 -3.55
Eisosomes -1.67 -14.01 -10.53 -9.43 -2.26 5.77 -17.63 -21.90 -27.12 -2.30 0.98 -24.25 -26.93 -25.80 -1.57
Endoplasmic Reticulum 0.47 -12.10 -6.71 -6.73 0.81 4.44 -16.25 -4.40 -4.90 0.08 3.33 -21.41 -5.33 -5.55 0.59
Endosome -2.66 -10.00 -4.61 -3.90 -0.52 3.60 -5.43 -5.25 -6.47 -3.49 -0.19 -13.90 -7.12 -6.96 -2.81
Golgi -2.64 0.86 2.72 4.13 3.40 0.70 2.48 -0.96 -1.70 -3.26 -0.65 3.24 -2.01 -1.61 -3.18
Lipid Particles -4.40 -10.01 -4.21 -3.06 1.20 4.27 -7.22 -7.52 -12.93 -0.54 -0.68 -14.86 -12.38 -11.24 2.06
Mitochondria -1.52 -4.84 -0.26 1.46 4.72 0.08 -3.03 -2.73 -2.71 -2.27 -1.35 -6.17 -2.90 -2.63 -2.09
Mitotic Spindle -1.99 -3.64 -1.00 0.16 2.18 -3.51 -2.30 -6.71 -5.27 -5.52 -4.69 -4.44 -7.00 -5.36 -4.87
None 0.52 10.29 13.21 8.49 3.01 1.99 7.77 7.55 8.95 0.38 1.90 11.92 10.59 10.08 0.44
Nuclear Periphery -5.20 -21.53 -9.85 -9.09 3.31 0.57 -27.97 -17.89 -18.27 -0.04 -3.49 -35.86 -19.98 -19.42 1.11
Nuclear Periphery Foci -3.92 -5.92 -2.29 -1.19 -0.43 5.64 5.38 5.41 -1.42 0.29 1.84 -0.36 1.13 -0.70 1.44
Nucleolus 0.13 -5.48 -2.09 -2.39 4.22 2.38 -1.73 -1.02 -1.50 -0.69 1.02 -6.47 -1.42 -1.57 -0.30
Nucleus -14.30 -18.33 -2.81 2.69 8.71 -1.53 -28.23 -7.11 -6.67 18.62 -12.61 -39.92 -12.47 -4.90 19.86
Peroxisomes -1.75 -5.39 -2.31 0.80 -0.28 0.47 -2.54 -3.07 -3.17 -2.61 -1.81 -7.43 -3.67 -1.17 -2.32
Vacuole -4.99 -14.58 -12.24 -12.06 -5.71 3.71 -18.75 -25.91 -26.53 -12.86 -0.77 -24.04 -28.69 -28.62 -12.64
Vacuole Periphery 1.73 1.35 1.85 0.66 3.74 1.55 1.82 0.45 -1.87 -2.40 1.96 2.30 -0.04 -2.01 -2.35
Standard name
Human Ortholog
Description Protein of unconfirmed function; involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Sno1

Sno1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sno1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available