Standard name | |
---|---|
Human Ortholog | |
Description | Type I membrane protein involved in autophagy and the Cvt pathway; may be involved in membrane delivery to the phagophore assembly site |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 7 | 2 | 18 | 25 | 2 | 11 | 27 | 5 | 10 | 4 | 9 | 35 | 32 | 28 | 14 | 7 | 2 | 1 | 0 | 1 | 3 | 6 | 7 |
Bud | 0 | 1 | 0 | 0 | 4 | 2 | 1 | 16 | 7 | 10 | 4 | 4 | 2 | 1 | 4 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 3 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 155 | 89 | 39 | 60 | 135 | 29 | 140 | 209 | 66 | 124 | 63 | 91 | 74 | 53 | 91 | 34 | 16 | 5 | 17 | 9 | 2 | 3 | 8 | 10 |
Endoplasmic Reticulum | 31 | 25 | 24 | 30 | 22 | 9 | 31 | 7 | 2 | 15 | 1 | 12 | 105 | 90 | 50 | 6 | 2 | 1 | 25 | 11 | 7 | 30 | 19 | 29 |
Endosome | 62 | 3 | 18 | 28 | 62 | 9 | 42 | 84 | 13 | 30 | 10 | 30 | 36 | 86 | 106 | 21 | 17 | 2 | 153 | 61 | 49 | 162 | 193 | 196 |
Golgi | 21 | 9 | 15 | 33 | 18 | 0 | 5 | 3 | 0 | 1 | 0 | 0 | 144 | 245 | 254 | 29 | 17 | 17 | 45 | 22 | 19 | 157 | 148 | 233 |
Mitochondria | 3 | 3 | 0 | 1 | 0 | 17 | 1 | 8 | 13 | 17 | 17 | 21 | 5 | 17 | 15 | 7 | 6 | 0 | 7 | 3 | 4 | 9 | 6 | 4 |
Nucleus | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 2 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 1 | 1 | 3 | 2 | 2 |
Nucleolus | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 3 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 3 | 1 | 3 | 0 | 2 | 2 | 1 | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 |
Vac/Vac Membrane | 273 | 125 | 55 | 30 | 70 | 16 | 45 | 71 | 14 | 25 | 13 | 28 | 82 | 151 | 159 | 47 | 44 | 14 | 249 | 163 | 63 | 92 | 96 | 75 |
Unique Cell Count | 460 | 229 | 130 | 161 | 281 | 69 | 244 | 368 | 107 | 204 | 102 | 174 | 378 | 538 | 570 | 117 | 89 | 38 | 514 | 281 | 152 | 470 | 494 | 566 |
Labelled Cell Count | 555 | 265 | 154 | 201 | 339 | 86 | 279 | 435 | 123 | 240 | 117 | 202 | 487 | 680 | 713 | 161 | 111 | 41 | 514 | 281 | 152 | 470 | 494 | 566 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.1 | 12.7 | 12.6 | 12.1 | 11.9 | 6.4 | 8.8 | 8.4 | 7.7 | 7.5 | 7.2 | 7.4 | 12.5 | 13.0 | 12.9 | 14.6 | 14.4 | 16.1 | 11.5 | 11.9 | 12.1 |
Std Deviation (1e-4) | 2.1 | 2.4 | 2.2 | 1.7 | 1.9 | 1.7 | 1.6 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 2.1 | 2.3 | 2.3 | 3.5 | 3.0 | 3.3 | 2.3 | 2.4 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.09 | -0.97 | -0.52 | -0.59 | -0.7 | -0.74 | -0.81 | -0.77 | -0.01 | 0.04 | 0.03 | 0.21 | 0.19 | 0.35 | -0.13 | -0.08 | -0.06 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.2 | 2.8 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 2.9 | 2.1 | 1.7 | 3.3 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | 3.4 | 1.7 | 5.0 | 5.3 | 4.9 | 5.5 | 4.8 | 3.9 | -2.5 | -6.0 | -3.7 | -0.2 | -2.0 | -2.1 |
Endoplasmic Reticulum | 0 | -3.2 | -1.0 | -1.5 | -6.7 | -4.1 | -3.1 | -4.3 | -3.1 | 2.1 | -0.5 | -3.2 | -3.2 | -3.6 | -2.4 |
Endosome | 0.8 | 2.0 | -0.2 | 0.8 | 2.2 | -0.4 | 0.2 | -0.9 | 0.8 | -1.4 | 0.6 | 1.3 | 0.9 | 1.0 | 0 |
Golgi | 2.0 | -1.8 | -2.9 | -3.9 | 0 | -3.6 | -4.6 | -3.5 | -4.6 | 5.6 | 7.1 | 7.0 | 2.7 | 1.6 | 4.6 |
Mitochondria | 0 | 0 | 5.9 | 0 | 0 | 4.1 | 3.4 | 4.8 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.4 | -3.6 | -2.7 | -5.0 | -5.2 | -4.9 | -6.3 | -4.9 | -5.1 | -4.6 | -3.2 | -3.2 | -0.3 | 1.0 | -0.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0764 | 6.5629 | 6.1725 | 5.3631 | 4.4039 | 5.6988 | 6.8614 | 8.4494 | 8.1109 | 8.1714 | 7.6611 | 8.4652 | 8.9119 | 10.398 | 9.6257 | 9.9273 | 9.2026 | 10.0704 |
Actin | 0.0159 | 0.0002 | 0.0078 | 0 | 0.0155 | 0.0001 | 0.0227 | 0 | 0.0102 | 0 | 0.0016 | 0.0007 | 0.0398 | 0.0011 | 0.0082 | 0.0019 | 0 | 0.0001 |
Bud | 0.0002 | 0.0006 | 0.0013 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0013 | 0.0001 | 0 | 0.0004 |
Bud Neck | 0.0003 | 0.0003 | 0.0006 | 0.0001 | 0.001 | 0.0001 | 0.001 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0071 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0004 |
Bud Periphery | 0.0002 | 0.0003 | 0.0008 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0002 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.001 | 0.0001 | 0 | 0.0004 |
Bud Site | 0.0004 | 0.0005 | 0.0017 | 0 | 0.0015 | 0.0001 | 0.0013 | 0.001 | 0.0009 | 0.0002 | 0.0004 | 0.0001 | 0.0026 | 0.0004 | 0.001 | 0.0002 | 0 | 0.0003 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.019 | 0.0465 | 0.0421 | 0.0096 | 0.008 | 0.0202 | 0.0311 | 0.0646 | 0.0321 | 0.0662 | 0.0165 | 0.0419 | 0.0605 | 0.0962 | 0.0356 | 0.0071 | 0.0038 | 0.0458 |
Cytoplasmic Foci | 0.0251 | 0.0308 | 0.0292 | 0.0094 | 0.0234 | 0.0192 | 0.0226 | 0.0214 | 0.0255 | 0.0075 | 0.0199 | 0.0126 | 0.0229 | 0.02 | 0.0165 | 0.0243 | 0.0141 | 0.0123 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0162 | 0.0099 | 0.012 | 0.0043 | 0.0136 | 0.0074 | 0.0199 | 0.0108 | 0.0105 | 0.0038 | 0.003 | 0.0126 | 0.035 | 0.0164 | 0.0129 | 0.0017 | 0.0025 | 0.0127 |
Endosome | 0.6553 | 0.6092 | 0.6068 | 0.6107 | 0.7284 | 0.6861 | 0.5195 | 0.4278 | 0.4965 | 0.3602 | 0.5259 | 0.4948 | 0.4255 | 0.4709 | 0.4354 | 0.4713 | 0.5013 | 0.4541 |
Golgi | 0.0567 | 0.0543 | 0.0606 | 0.0103 | 0.0609 | 0.0586 | 0.0338 | 0.012 | 0.0253 | 0.0063 | 0.014 | 0.0201 | 0.0519 | 0.0402 | 0.0472 | 0.0289 | 0.0419 | 0.0364 |
Lipid Particles | 0.0149 | 0.0018 | 0.003 | 0.0008 | 0.0082 | 0.0013 | 0.0117 | 0.0015 | 0.0094 | 0.0004 | 0.0184 | 0.0061 | 0.0193 | 0.0026 | 0.0063 | 0.0107 | 0.0004 | 0.0011 |
Mitochondria | 0.0134 | 0.0066 | 0.0096 | 0.0018 | 0.0023 | 0.0065 | 0.0163 | 0.0009 | 0.0134 | 0.0006 | 0.003 | 0.0027 | 0.0079 | 0.0073 | 0.0107 | 0.0039 | 0.0005 | 0.0097 |
None | 0.0013 | 0.0004 | 0.0097 | 0.0001 | 0.0005 | 0.0096 | 0.0028 | 0.0002 | 0.0008 | 0.0002 | 0.0004 | 0.0004 | 0.0027 | 0.0068 | 0.0005 | 0.0002 | 0 | 0.0004 |
Nuclear Periphery | 0.0174 | 0.0085 | 0.0182 | 0.0052 | 0.0057 | 0.0059 | 0.0382 | 0.0129 | 0.0185 | 0.0084 | 0.0035 | 0.0256 | 0.0756 | 0.023 | 0.0254 | 0.0042 | 0.008 | 0.0181 |
Nucleolus | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.001 | 0.0002 | 0.0007 | 0.0002 | 0.0009 | 0.0001 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0002 |
Nucleus | 0.001 | 0.0009 | 0.0015 | 0.0004 | 0.0005 | 0.0005 | 0.0107 | 0.0024 | 0.0022 | 0.0019 | 0.0009 | 0.0022 | 0.0231 | 0.0026 | 0.0031 | 0.0006 | 0.0005 | 0.0066 |
Peroxisomes | 0.0034 | 0.0004 | 0.0034 | 0 | 0.0043 | 0.0001 | 0.003 | 0.0001 | 0.0022 | 0 | 0.0101 | 0.0002 | 0.0013 | 0.0002 | 0.0006 | 0.0117 | 0 | 0.0001 |
Punctate Nuclear | 0.0009 | 0.0001 | 0.0007 | 0 | 0.002 | 0.0001 | 0.0042 | 0.0001 | 0.0008 | 0.0001 | 0.0041 | 0.0006 | 0.0038 | 0.0003 | 0.0002 | 0.0006 | 0 | 0.0002 |
Vacuole | 0.092 | 0.1447 | 0.1181 | 0.163 | 0.0674 | 0.0888 | 0.17 | 0.329 | 0.2353 | 0.3637 | 0.2606 | 0.2099 | 0.1419 | 0.2082 | 0.2465 | 0.2864 | 0.2513 | 0.192 |
Vacuole Periphery | 0.0658 | 0.0839 | 0.0724 | 0.1837 | 0.0557 | 0.0951 | 0.0887 | 0.1141 | 0.114 | 0.1797 | 0.1162 | 0.1685 | 0.0764 | 0.1027 | 0.1463 | 0.1458 | 0.1753 | 0.2086 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 60.8495 | 62.8573 | 62.7884 | 65.7837 | 64.8684 | 63.5187 | 59.8883 | 55.6814 | 71.9096 | 64.0543 |
Translational Efficiency | 1.2778 | 1.3181 | 1.288 | 1.4284 | 1.3054 | 1.2321 | 1.2483 | 1.4881 | 1.0406 | 1.1655 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
573 | 1180 | 226 | 1196 | 2150 | 1801 | 1399 | 1078 | 2723 | 2981 | 1625 | 2274 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 923.43 | 1150.06 | 1590.70 | 1517.13 | 982.54 | 1131.69 | 1412.81 | 1527.36 | 970.10 | 1138.96 | 1437.55 | 1521.98 |
Standard Deviation | 707.02 | 197.40 | 236.78 | 286.79 | 141.23 | 181.11 | 225.12 | 299.97 | 348.60 | 187.94 | 234.98 | 293.15 |
Intensity Change Log 2 | — | 0.316635 | 0.784587 | 0.716270 | — | 0.203891 | 0.523979 | 0.636452 | — | 0.259615 | 0.656124 | 0.675675 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000358 | 0.000736 | 0.000228 | 0.001853 | 0.000103 | 0.000383 | 0.000239 | 0.001748 | 0.000157 | 0.000523 | 0.000237 | 0.001803 |
Bud Neck | 0.002399 | 0.000369 | 0.002110 | 0.001102 | 0.000385 | 0.000668 | 0.000460 | 0.001041 | 0.000809 | 0.000549 | 0.000690 | 0.001073 |
Bud Site | 0.020101 | 0.007825 | 0.004769 | 0.021807 | 0.004056 | 0.007177 | 0.007443 | 0.020959 | 0.007432 | 0.007434 | 0.007071 | 0.021405 |
Cell Periphery | 0.001900 | 0.000528 | 0.000281 | 0.002065 | 0.000232 | 0.000507 | 0.000461 | 0.002018 | 0.000583 | 0.000516 | 0.000436 | 0.002043 |
Cytoplasm | 0.026581 | 0.005370 | 0.001056 | 0.012093 | 0.004072 | 0.006924 | 0.003399 | 0.009149 | 0.008809 | 0.006309 | 0.003073 | 0.010697 |
Cytoplasmic Foci | 0.256535* | 0.106486 | 0.032328 | 0.058712 | 0.119262 | 0.050886 | 0.036365 | 0.073682 | 0.148149 | 0.072895 | 0.035803 | 0.065809 |
Eisosomes | 0.000113 | 0.000017 | 0.000010 | 0.000037 | 0.000019 | 0.000021 | 0.000012 | 0.000035 | 0.000039 | 0.000020 | 0.000012 | 0.000036 |
Endoplasmic Reticulum | 0.029778 | 0.006198 | 0.003871 | 0.006118 | 0.008962 | 0.004565 | 0.006339 | 0.005997 | 0.013343 | 0.005211 | 0.005996 | 0.006061 |
Endosome | 0.173902 | 0.183428 | 0.111741 | 0.114104 | 0.218698* | 0.188213 | 0.108606 | 0.130971 | 0.209272* | 0.186319 | 0.109042 | 0.122100 |
Golgi | 0.124204* | 0.232151* | 0.189587* | 0.480174* | 0.176505* | 0.099926 | 0.334288* | 0.497690* | 0.165499* | 0.152266* | 0.314163* | 0.488477* |
Lipid Particles | 0.005134 | 0.001563 | 0.005886 | 0.012426 | 0.002239 | 0.001455 | 0.004248 | 0.015105 | 0.002848 | 0.001498 | 0.004475 | 0.013696 |
Mitochondria | 0.014245 | 0.003342 | 0.000390 | 0.001241 | 0.008757 | 0.006291 | 0.001446 | 0.001393 | 0.009912 | 0.005123 | 0.001299 | 0.001313 |
Mitotic Spindle | 0.000642 | 0.002746 | 0.007758 | 0.003578 | 0.000536 | 0.001235 | 0.001850 | 0.002338 | 0.000558 | 0.001833 | 0.002671 | 0.002990 |
None | 0.000132 | 0.000129 | 0.000022 | 0.000053 | 0.000087 | 0.000520 | 0.000043 | 0.000049 | 0.000097 | 0.000366 | 0.000040 | 0.000051 |
Nuclear Periphery | 0.000122 | 0.000199 | 0.003481 | 0.000190 | 0.000312 | 0.000724 | 0.000555 | 0.000268 | 0.000272 | 0.000516 | 0.000962 | 0.000227 |
Nuclear Periphery Foci | 0.000420 | 0.001315 | 0.023177 | 0.004095 | 0.001348 | 0.002478 | 0.005012 | 0.003753 | 0.001153 | 0.002018 | 0.007538 | 0.003933 |
Nucleolus | 0.000055 | 0.000051 | 0.000602 | 0.000025 | 0.000059 | 0.000222 | 0.000029 | 0.000033 | 0.000058 | 0.000154 | 0.000109 | 0.000029 |
Nucleus | 0.002019 | 0.000090 | 0.000157 | 0.000107 | 0.000126 | 0.000291 | 0.000048 | 0.000070 | 0.000524 | 0.000212 | 0.000063 | 0.000089 |
Peroxisomes | 0.001180 | 0.000307 | 0.000375 | 0.000465 | 0.000254 | 0.000479 | 0.000100 | 0.000628 | 0.000449 | 0.000411 | 0.000138 | 0.000542 |
Vacuole | 0.103116 | 0.212654* | 0.417235* | 0.194905 | 0.200973* | 0.332436* | 0.332838* | 0.164819 | 0.180381 | 0.285022* | 0.344576* | 0.180642 |
Vacuole Periphery | 0.237065* | 0.234497* | 0.194937* | 0.084854 | 0.253013* | 0.294596* | 0.156222* | 0.068254 | 0.249657* | 0.270806* | 0.161606* | 0.076985 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.73 | 1.31 | -3.32 | -1.66 | -3.62 | -1.07 | -1.70 | -5.18 | -3.40 | -4.64 | -1.42 | -1.16 | -5.94 | -3.44 | -5.51 |
Bud Neck | 3.64 | 0.33 | 2.18 | -4.53 | 2.34 | -2.62 | -1.16 | -5.63 | -2.84 | -5.03 | 1.84 | 0.72 | -1.94 | -4.82 | -3.36 |
Bud Site | 5.15 | 5.56 | -0.90 | -7.03 | -7.14 | -4.10 | -3.85 | -8.70 | -6.80 | -6.39 | -0.03 | 0.12 | -9.97 | -9.87 | -9.29 |
Cell Periphery | 2.17 | 2.70 | -0.23 | -3.41 | -4.41 | -1.75 | -5.63 | -4.94 | -3.84 | -4.29 | 0.38 | 1.11 | -4.86 | -5.11 | -5.86 |
Cytoplasm | 7.26 | 8.90 | 4.08 | -4.23 | -6.94 | -3.02 | 0.87 | -4.17 | -1.67 | -4.40 | 2.69 | 6.71 | -2.03 | -4.10 | -7.00 |
Cytoplasmic Foci | 15.92 | 23.71 | 21.96 | 10.80 | -5.93 | 18.57 | 24.36 | 10.94 | -6.64 | -11.88 | 19.94 | 32.13 | 22.17 | 2.57 | -13.15 |
Eisosomes | 5.08 | 5.49 | 3.98 | -3.96 | -6.05 | -0.27 | 3.06 | -4.97 | -1.57 | -6.96 | 2.79 | 6.10 | 0.46 | -2.80 | -8.47 |
Endoplasmic Reticulum | 7.15 | 7.67 | 7.23 | 0.04 | -1.87 | 4.54 | 2.17 | 2.34 | -1.38 | 0.29 | 7.75 | 6.04 | 6.54 | -1.27 | -0.07 |
Endosome | -1.13 | 5.36 | 7.91 | 12.02 | -0.21 | 4.82 | 21.04 | 15.56 | 9.45 | -4.56 | 4.59 | 21.31 | 19.95 | 15.23 | -3.35 |
Golgi | -11.06 | -3.90 | -32.29* | -22.03 | -16.48 | 12.66 | -17.23 | -30.99* | -39.73* | -13.38 | 2.41 | -18.05 | -43.16* | -45.39* | -18.04 |
Lipid Particles | 4.50 | -0.44 | -5.29 | -9.11 | -3.37 | 2.19 | -3.22 | -6.73 | -7.12 | -5.50 | 4.32 | -2.75 | -9.70 | -11.02 | -7.61 |
Mitochondria | 6.69 | 8.78 | 8.16 | 4.45 | -3.42 | 2.54 | 9.32 | 10.13 | 6.64 | 0.13 | 6.18 | 12.01 | 13.14 | 7.93 | -0.02 |
Mitotic Spindle | -1.77 | -2.83 | -3.31 | -0.80 | 1.41 | -2.44 | -3.45 | -5.09 | -2.78 | -1.10 | -2.54 | -4.41 | -4.81 | -1.93 | -0.74 |
None | 0.04 | 2.72 | 1.89 | 1.46 | -6.25 | -1.04 | 2.99 | 2.54 | 1.14 | -1.04 | -1.07 | 3.90 | 3.06 | 1.25 | -2.77 |
Nuclear Periphery | -2.09 | -4.42 | -3.18 | 0.10 | 4.33 | -2.22 | -2.92 | 0.66 | 2.42 | 3.32 | -2.13 | -5.34 | 1.00 | 2.53 | 5.74 |
Nuclear Periphery Foci | -5.06 | -4.30 | -6.03 | -4.50 | 3.55 | -1.60 | -6.40 | -3.28 | -1.47 | 1.45 | -1.87 | -7.16 | -5.45 | -3.39 | 3.62 |
Nucleolus | 0.29 | -1.04 | 3.15 | 2.63 | 1.10 | -3.02 | 6.21 | 3.60 | 3.49 | -0.74 | -2.92 | -0.69 | 5.85 | 3.81 | 1.09 |
Nucleus | 1.15 | 1.11 | 1.12 | -0.72 | 0.29 | -2.49 | 5.63 | 3.29 | 3.32 | -2.55 | 0.88 | 1.30 | 1.18 | 2.13 | -1.25 |
Peroxisomes | 4.60 | 3.57 | 3.41 | -1.55 | -0.58 | -1.39 | 5.95 | -3.50 | -0.80 | -4.95 | 0.34 | 6.42 | -1.19 | -1.11 | -5.47 |
Vacuole | -13.94 | -17.69 | -11.10 | 1.93 | 12.26 | -19.16 | -16.50 | 4.53 | 19.96 | 17.96 | -19.14 | -22.50 | -0.28 | 16.98 | 20.97 |
Vacuole Periphery | 0.23 | 2.61 | 15.18 | 21.30 | 8.00 | -5.72 | 15.17 | 32.64* | 36.82* | 17.11* | -3.52 | 14.91 | 35.70 | 41.45* | 18.24* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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