Standard name | |
---|---|
Human Ortholog | |
Description | One of two type I myosin motors; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 201 | 25 | 78 | 80 | 86 | 79 | 93 | 80 | 47 | 71 | 60 | 89 | 87 | 122 | 143 | 185 | 100 | 151 | 299 | 64 | 99 | 56 | 125 | 126 |
Bud | 56 | 9 | 27 | 25 | 38 | 44 | 36 | 41 | 23 | 30 | 28 | 43 | 28 | 34 | 49 | 61 | 31 | 66 | 32 | 13 | 4 | 16 | 31 | 27 |
Bud Neck | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 7 | 3 | 3 | 9 | 6 |
Bud Site | 12 | 3 | 12 | 16 | 19 | 13 | 12 | 10 | 5 | 11 | 9 | 12 | 8 | 4 | 2 | 18 | 5 | 10 | – | – | – | – | – | – |
Cell Periphery | 29 | 2 | 4 | 13 | 8 | 10 | 12 | 6 | 7 | 10 | 6 | 18 | 17 | 18 | 32 | 32 | 21 | 12 | 2 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 29 | 8 | 7 | 2 | 12 | 13 | 13 | 13 | 12 | 8 | 7 | 19 | 11 | 15 | 16 | 55 | 20 | 34 | 5 | 2 | 2 | 1 | 3 | 0 |
Endoplasmic Reticulum | 5 | 4 | 4 | 4 | 7 | 12 | 10 | 13 | 5 | 4 | 3 | 5 | 3 | 9 | 6 | 6 | 1 | 5 | 1 | 0 | 1 | 0 | 1 | 0 |
Endosome | 6 | 3 | 1 | 1 | 6 | 2 | 4 | 5 | 1 | 1 | 3 | 3 | 2 | 5 | 3 | 4 | 0 | 2 | 5 | 9 | 4 | 0 | 2 | 3 |
Golgi | 65 | 0 | 2 | 2 | 3 | 3 | 8 | 4 | 2 | 4 | 3 | 2 | 5 | 9 | 9 | 79 | 59 | 60 | 1 | 2 | 0 | 0 | 2 | 2 |
Mitochondria | 21 | 22 | 61 | 90 | 43 | 88 | 129 | 132 | 83 | 118 | 89 | 155 | 41 | 46 | 71 | 25 | 16 | 25 | 0 | 2 | 3 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 2 | 0 | 2 | 3 | 0 | 1 | 0 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 2 | 0 | 3 | 4 | 3 | 1 | 2 | 1 | 3 | 0 | 4 | 2 | 1 | 1 | 4 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 24 | 5 | 31 | 25 | 40 | 25 | 30 | 28 | 21 | 40 | 19 | 33 | 41 | 44 | 51 | 13 | 6 | 8 | 10 | 2 | 12 | 0 | 2 | 4 |
SpindlePole | 13 | 0 | 1 | 1 | 0 | 1 | 3 | 2 | 2 | 1 | 1 | 4 | 2 | 1 | 2 | 9 | 8 | 6 | 1 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | 5 | 3 | 2 | 1 | 2 | 5 | 4 | 4 | 3 | 2 | 2 | 2 | 0 | 0 | 1 | 12 | 11 | 1 | 0 | 0 | 1 | 0 | 0 | 1 |
Unique Cell Count | 289 | 49 | 116 | 120 | 139 | 160 | 202 | 208 | 132 | 177 | 126 | 238 | 137 | 166 | 203 | 313 | 179 | 242 | 372 | 109 | 138 | 83 | 184 | 176 |
Labelled Cell Count | 469 | 86 | 230 | 264 | 271 | 298 | 358 | 345 | 214 | 304 | 230 | 395 | 248 | 309 | 388 | 503 | 284 | 382 | 372 | 109 | 138 | 83 | 184 | 176 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.6 | 9.5 | 8.1 | 8.7 | 8.7 | 8.5 | 7.8 | 7.3 | 7.4 | 7.4 | 7.2 | 7.1 | 11.8 | 12.1 | 10.7 | 16.3 | 18.2 | 18.1 | – | 12.5 | 13.4 |
Std Deviation (1e-4) | 3.4 | 2.5 | 2.4 | 2.8 | 1.9 | 2.1 | 1.7 | 1.5 | 1.6 | 1.5 | 1.5 | 1.7 | 2.6 | 2.7 | 2.5 | 5.1 | 6.0 | 5.9 | – | 2.4 | 2.5 |
Intensity Change (Log2) | – | – | – | 0.1 | 0.1 | 0.07 | -0.05 | -0.15 | -0.13 | -0.14 | -0.17 | -0.2 | 0.54 | 0.57 | 0.39 | 1.01 | 1.16 | 1.16 | – | 0.62 | 0.72 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.1 | -0.9 | -3.0 | -3.7 | -5.0 | -5.0 | -4.5 | -3.1 | -5.3 | -0.6 | 1.1 | 0.6 | -1.5 | -2.0 | -0.9 |
Bud | -0.5 | 0.7 | 0.8 | -1.2 | -0.8 | -1.1 | -1.3 | -0.2 | -1.2 | -0.5 | -0.6 | 0.2 | -0.9 | -1.3 | 0.8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.7 | 0.8 | -0.6 | -1.4 | -1.9 | -2.0 | -1.3 | -0.9 | -1.9 | -1.3 | -2.8 | -3.9 | -1.7 | -2.7 | -2.3 |
Cell Periphery | 2.2 | 0.9 | 1.0 | 1.0 | 0 | 0.7 | 0.9 | 0 | 1.5 | 2.6 | 2.3 | 3.3 | 2.2 | 2.5 | 0.6 |
Cytoplasm | 0 | 0.8 | 0.7 | 0.1 | 0.1 | 0.9 | -0.6 | -0.2 | 0.7 | 0.6 | 0.9 | 0.6 | 3.0 | 1.5 | 2.2 |
Endoplasmic Reticulum | 0 | 0.6 | 1.4 | 0.6 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.5 | 6.5 | 5.4 |
Mitochondria | 3.6 | -3.5 | 0.4 | 2.0 | 1.9 | 1.6 | 2.4 | 2.9 | 2.3 | -3.7 | -4.2 | -3.1 | -10.2 | -8.3 | -8.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -1.1 | 0.4 | -2.3 | -2.6 | -3.0 | -2.1 | -0.8 | -2.2 | -3.0 | 0.6 | 0 | -0.3 | -6.8 | -5.9 | -6.7 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.8628 | 11.1831 | 10.8889 | 10.4473 | 9.5316 | 11.8988 | 10.2397 | 11.6589 | 10.8467 | 10.2379 | 10.6233 | 11.1504 | 10.4948 | 10.5893 | 10.0452 | 10.1695 | 10.9663 | 10.5942 |
Actin | 0.9686 | 0.8378 | 0.9116 | 0.9543 | 0.938 | 0.9544 | 0.7949 | 0.731 | 0.9081 | 0.7978 | 0.9336 | 0.8777 | 0.8024 | 0.8555 | 0.8767 | 0.9227 | 0.9944 | 0.9466 |
Bud | 0.0004 | 0.0018 | 0.0158 | 0.001 | 0.0006 | 0.0002 | 0.0095 | 0.006 | 0.0052 | 0.0204 | 0.0007 | 0.0016 | 0.0007 | 0.0096 | 0.0138 | 0.0037 | 0.0009 | 0.0003 |
Bud Neck | 0.0032 | 0.0074 | 0.0148 | 0.02 | 0.0032 | 0.0277 | 0.0046 | 0.0052 | 0.0077 | 0.0174 | 0.0199 | 0.0691 | 0.0215 | 0.0042 | 0.0035 | 0.0014 | 0.0003 | 0.0325 |
Bud Periphery | 0.0004 | 0.0019 | 0.0155 | 0.0021 | 0.001 | 0.0002 | 0.0115 | 0.0055 | 0.0071 | 0.0689 | 0.0012 | 0.0038 | 0.0007 | 0.0091 | 0.0207 | 0.0069 | 0.0014 | 0.0004 |
Bud Site | 0.0163 | 0.1089 | 0.0368 | 0.0109 | 0.0013 | 0.0007 | 0.031 | 0.2329 | 0.0287 | 0.0044 | 0.0013 | 0.0008 | 0.0366 | 0.1126 | 0.0291 | 0.0051 | 0.0017 | 0.0017 |
Cell Periphery | 0.0002 | 0.0013 | 0.0005 | 0.0009 | 0.0009 | 0.0001 | 0.0008 | 0.0006 | 0.0008 | 0.0012 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.0002 | 0.0125 | 0.0018 | 0.001 | 0.0256 | 0.0001 | 0.01 | 0.0018 | 0.0046 | 0.0084 | 0.0004 | 0.0005 | 0.0043 | 0.0003 | 0.0006 | 0.0004 | 0 | 0.0005 |
Cytoplasmic Foci | 0.0017 | 0.01 | 0.0006 | 0.0018 | 0.0017 | 0.002 | 0.0181 | 0.0083 | 0.0116 | 0.0134 | 0.0016 | 0.0022 | 0.0227 | 0.0012 | 0.0227 | 0.0049 | 0.0001 | 0.0058 |
Eisosomes | 0.002 | 0.0009 | 0.0006 | 0.0009 | 0.0008 | 0.0037 | 0.0019 | 0.001 | 0.0005 | 0.0005 | 0.0005 | 0.0012 | 0.0054 | 0.0013 | 0.0006 | 0.0007 | 0.0003 | 0.0011 |
Endoplasmic Reticulum | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0129 | 0.0001 | 0.0187 | 0.0001 | 0.0005 | 0.0028 | 0.0001 | 0.0005 | 0.0011 | 0 | 0.0003 | 0.0001 | 0 | 0.0003 |
Endosome | 0.0003 | 0.0014 | 0.0001 | 0.0004 | 0.0043 | 0.0008 | 0.0297 | 0.0004 | 0.0075 | 0.0228 | 0.0052 | 0.016 | 0.0071 | 0 | 0.0035 | 0.004 | 0 | 0.002 |
Golgi | 0.0022 | 0.0059 | 0.0008 | 0.0041 | 0.0017 | 0.0057 | 0.0121 | 0.0006 | 0.0038 | 0.0172 | 0.0113 | 0.0109 | 0.0071 | 0.0007 | 0.004 | 0.0121 | 0.0002 | 0.0019 |
Lipid Particles | 0.0004 | 0.002 | 0.0001 | 0.0002 | 0.0013 | 0.0011 | 0.0075 | 0.0004 | 0.0007 | 0.0035 | 0.0001 | 0.0008 | 0.0125 | 0.0001 | 0.0044 | 0.0012 | 0 | 0.0008 |
Mitochondria | 0.0006 | 0.0022 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.011 | 0.0002 | 0.0046 | 0.002 | 0.0227 | 0.0098 | 0.0051 | 0.0001 | 0.0043 | 0.034 | 0.0001 | 0.0012 |
None | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0018 | 0.0027 | 0.0002 | 0.0015 | 0 | 0.0001 | 0.013 | 0.0001 | 0.0005 | 0 | 0 | 0.0002 |
Nuclear Periphery | 0 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.02 | 0 | 0.0005 | 0.0031 | 0 | 0.0004 | 0.0142 | 0 | 0.0008 | 0 | 0 | 0.0002 |
Nucleolus | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0.0014 | 0 | 0.0004 | 0 | 0 | 0 |
Nucleus | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.002 | 0.0001 | 0.0008 | 0.0007 | 0 | 0.0001 | 0.0052 | 0 | 0.0005 | 0 | 0 | 0.0001 |
Peroxisomes | 0.0034 | 0.0051 | 0.0008 | 0.0018 | 0.0028 | 0.0024 | 0.0073 | 0.0019 | 0.0031 | 0.001 | 0.0007 | 0.0025 | 0.0317 | 0.0045 | 0.0112 | 0.0021 | 0.0004 | 0.0016 |
Punctate Nuclear | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0014 | 0.0011 | 0.001 | 0.0007 | 0 | 0.0001 | 0.0055 | 0.0001 | 0.0015 | 0.0001 | 0 | 0.0025 |
Vacuole | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0025 | 0 | 0.0032 | 0.0001 | 0.0024 | 0.0084 | 0.0001 | 0.0012 | 0.0007 | 0 | 0.0004 | 0.0002 | 0 | 0.0001 |
Vacuole Periphery | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0027 | 0 | 0.0004 | 0.0037 | 0.0002 | 0.0007 | 0.0008 | 0 | 0.0002 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.5634 | 35.0352 | 43.996 | 55.0367 | 40.4558 | 41.2794 | 52.3868 | 58.6687 | 58.4171 | 56.5468 |
Translational Efficiency | 0.6713 | 0.7051 | 0.6011 | 0.5741 | 0.5754 | 0.6955 | 0.5872 | 0.4862 | 0.5201 | 0.5387 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1519 | 36 | 158 | 1255 | 2144 | 2280 | 123 | 2302 | 3663 | 2316 | 281 | 3557 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1114.49 | 321.03 | 2052.24 | 1568.34 | 975.49 | 1137.00 | 1687.57 | 1579.04 | 1033.13 | 1124.32 | 1892.62 | 1575.26 |
Standard Deviation | 206.80 | 4.05 | 283.93 | 264.14 | 190.78 | 202.22 | 186.82 | 264.67 | 209.11 | 224.61 | 305.51 | 264.53 |
Intensity Change Log 2 | — | -1.795604 | 0.880816 | 0.492855 | — | 0.221033 | 0.790748 | 0.694849 | — | -0.519469 | 0.839476 | 0.590662 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.799610* | 0.000020 | 0.727839* | 0.668525* | 0.779523* | 0.772952* | 0.620068* | 0.690167* | 0.787852* | 0.760938* | 0.680665* | 0.682532* |
Bud Neck | 0.014027 | 0.000004 | 0.029253 | 0.012920 | 0.005888 | 0.013238 | 0.026666 | 0.013627 | 0.009263 | 0.013033 | 0.028121 | 0.013378 |
Bud Site | 0.087334 | 0.000009 | 0.152336* | 0.205350* | 0.060176 | 0.120361* | 0.304335* | 0.215271* | 0.071438 | 0.118490* | 0.218869* | 0.211771* |
Cell Periphery | 0.005868 | 0.000036 | 0.003469 | 0.022721 | 0.003077 | 0.006521 | 0.002537 | 0.018093 | 0.004234 | 0.006420 | 0.003061 | 0.019726 |
Cytoplasm | 0.006817 | 0.000021 | 0.005554 | 0.006492 | 0.008618 | 0.010298 | 0.005110 | 0.016259 | 0.007871 | 0.010138 | 0.005360 | 0.012813 |
Cytoplasmic Foci | 0.020916 | 0.000012 | 0.009139 | 0.002018 | 0.029935 | 0.017398 | 0.007157 | 0.001898 | 0.026195 | 0.017127 | 0.008271 | 0.001940 |
Eisosomes | 0.006938 | 0.000032 | 0.043850 | 0.067744 | 0.003574 | 0.004696 | 0.006505 | 0.030949 | 0.004969 | 0.004624 | 0.027503 | 0.043931 |
Endoplasmic Reticulum | 0.000256 | 0.000000 | 0.004556 | 0.002378 | 0.000102 | 0.000345 | 0.001635 | 0.000509 | 0.000166 | 0.000339 | 0.003277 | 0.001168 |
Endosome | 0.005599 | 0.000001 | 0.003429 | 0.002392 | 0.005874 | 0.004898 | 0.004198 | 0.001673 | 0.005760 | 0.004822 | 0.003766 | 0.001927 |
Golgi | 0.003936 | 0.000004 | 0.004594 | 0.002074 | 0.004556 | 0.003445 | 0.001226 | 0.002259 | 0.004299 | 0.003392 | 0.003120 | 0.002194 |
Lipid Particles | 0.004763 | 0.000019 | 0.005237 | 0.001553 | 0.005397 | 0.000617 | 0.002402 | 0.000285 | 0.005134 | 0.000608 | 0.003996 | 0.000732 |
Mitochondria | 0.002628 | 0.000007 | 0.000442 | 0.001986 | 0.003519 | 0.003332 | 0.000883 | 0.003655 | 0.003150 | 0.003281 | 0.000635 | 0.003066 |
Mitotic Spindle | 0.000214 | 0.000310 | 0.001249 | 0.000561 | 0.000279 | 0.001354 | 0.000227 | 0.000955 | 0.000252 | 0.001338 | 0.000802 | 0.000816 |
None | 0.000102 | 0.999403* | 0.000023 | 0.000032 | 0.000162 | 0.000119 | 0.000013 | 0.000036 | 0.000138 | 0.015651 | 0.000019 | 0.000034 |
Nuclear Periphery | 0.000004 | 0.000000 | 0.000045 | 0.000023 | 0.000002 | 0.000006 | 0.000018 | 0.000014 | 0.000003 | 0.000006 | 0.000033 | 0.000017 |
Nuclear Periphery Foci | 0.000044 | 0.000028 | 0.000341 | 0.000027 | 0.000011 | 0.000063 | 0.000277 | 0.000037 | 0.000025 | 0.000063 | 0.000313 | 0.000034 |
Nucleolus | 0.000013 | 0.000026 | 0.000005 | 0.000004 | 0.000011 | 0.000088 | 0.000004 | 0.000005 | 0.000012 | 0.000087 | 0.000005 | 0.000004 |
Nucleus | 0.000378 | 0.000008 | 0.000253 | 0.000079 | 0.000485 | 0.000162 | 0.000059 | 0.000049 | 0.000440 | 0.000159 | 0.000168 | 0.000060 |
Peroxisomes | 0.040406 | 0.000058 | 0.004159 | 0.001649 | 0.088726 | 0.039035 | 0.009733 | 0.002446 | 0.068688 | 0.038429 | 0.006599 | 0.002165 |
Vacuole | 0.000118 | 0.000001 | 0.004129 | 0.001072 | 0.000068 | 0.000676 | 0.006918 | 0.001183 | 0.000089 | 0.000666 | 0.005350 | 0.001144 |
Vacuole Periphery | 0.000030 | 0.000000 | 0.000097 | 0.000400 | 0.000016 | 0.000394 | 0.000030 | 0.000629 | 0.000022 | 0.000388 | 0.000068 | 0.000548 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 108.00* | 3.24 | 10.79 | -68.67* | 1.77 | 0.70 | 4.76 | 9.09 | 8.46 | -2.09 | 3.19 | 5.49 | 13.81 | 8.80 | -0.42 |
Bud Neck | 10.36 | -3.29 | 0.68 | -14.00 | 3.61 | -6.09 | -3.60 | -7.25 | -0.28 | 2.24 | -3.07 | -5.24 | -4.39 | -0.27 | 4.11 |
Bud Site | 18.00* | -3.46 | -12.14 | -24.33* | -2.38 | -10.11 | -7.97 | -21.65 | -12.07 | 2.88 | -8.49 | -8.35 | -24.25 | -13.55 | 0.50 |
Cell Periphery | 7.26 | 2.44 | -8.22 | -12.04 | -9.82 | -5.00 | 0.94 | -10.84 | -7.85 | -10.93 | -3.09 | 2.16 | -13.33 | -10.67 | -14.44 |
Cytoplasm | 6.69 | -0.52 | -0.03 | -6.81 | 0.51 | -1.30 | 1.81 | -4.37 | -3.37 | -4.97 | -1.96 | 0.20 | -4.11 | -2.07 | -2.15 |
Cytoplasmic Foci | 9.77 | 2.30 | 8.50 | -3.58 | 2.59 | 4.60 | 6.73 | 12.56 | 9.36 | 2.05 | 4.08 | 5.99 | 15.17 | 9.39 | 3.28 |
Eisosomes | 8.82 | -4.21 | -12.86 | -14.53 | -2.55 | -1.74 | -1.59 | -11.99 | -11.38 | -8.52 | 0.54 | -4.42 | -17.30 | -17.30 | -3.12 |
Endoplasmic Reticulum | 8.22 | -1.95 | -4.30 | -4.83 | 0.96 | -2.28 | -1.78 | -4.72 | -1.19 | 1.30 | -1.65 | -2.41 | -5.47 | -3.93 | 1.61 |
Endosome | 6.10 | 1.44 | 2.89 | -3.95 | 0.99 | 0.82 | 0.98 | 4.40 | 3.89 | 1.73 | 0.93 | 1.76 | 5.35 | 3.56 | 2.13 |
Golgi | 7.00 | -0.27 | 2.22 | -3.40 | 0.97 | 1.35 | 4.49 | 2.69 | 1.58 | -1.60 | 1.36 | 0.76 | 3.43 | 1.84 | 0.62 |
Lipid Particles | 4.29 | -0.61 | 2.66 | -3.47 | 2.56 | 5.00 | 2.33 | 5.37 | 2.66 | 2.56 | 6.21 | 0.57 | 5.94 | -0.68 | 3.62 |
Mitochondria | 5.41 | 4.40 | 0.77 | -2.95 | -2.28 | 0.23 | 3.93 | -0.18 | -0.41 | -3.88 | -0.21 | 5.93 | 0.12 | 0.30 | -4.90 |
Mitotic Spindle | -1.61 | -1.48 | -1.74 | -1.26 | 1.10 | -2.40 | 0.55 | -2.02 | 0.70 | -2.21 | -2.48 | -1.42 | -2.49 | 1.04 | 0.36 |
None | -11505.74* | 5.19 | 4.80 | 11663.24* | -1.45 | 0.64 | 5.73 | 4.84 | 1.31 | -7.77 | -6.04 | 7.12 | 6.26 | 6.08 | -5.17 |
Nuclear Periphery | 5.05 | -3.59 | -4.46 | -5.41 | 2.13 | -2.31 | -3.08 | -4.56 | -2.70 | 0.71 | -1.80 | -4.29 | -6.31 | -4.23 | 2.48 |
Nuclear Periphery Foci | 0.70 | -1.52 | 0.71 | 0.05 | 1.58 | -2.28 | -1.09 | -1.73 | 0.93 | 0.98 | -1.49 | -1.88 | -0.56 | 1.17 | 1.84 |
Nucleolus | -1.09 | 0.65 | 0.95 | 4.17 | 1.19 | -1.30 | 0.98 | 0.83 | 1.42 | -0.81 | -1.29 | 1.07 | 1.25 | 1.43 | 0.64 |
Nucleus | 1.11 | -0.11 | 0.89 | -4.34 | 2.01 | 0.70 | 0.94 | 0.96 | 1.14 | 0.83 | 0.91 | 0.56 | 1.28 | 1.01 | 2.11 |
Peroxisomes | 12.65 | 10.33 | 12.00 | -3.43 | 1.66 | 10.08 | 12.73 | 20.60 | 13.67 | 1.58 | 7.92 | 17.50 | 23.58 | 13.83 | 2.03 |
Vacuole | 5.83 | -2.99 | -2.12 | -2.36 | 2.41 | -3.16 | -1.20 | -3.58 | -1.46 | 1.00 | -3.04 | -2.35 | -4.09 | -1.63 | 1.94 |
Vacuole Periphery | 3.71 | -1.80 | -1.66 | -1.79 | -1.31 | -3.04 | -1.28 | -2.92 | -0.98 | -2.85 | -2.99 | -2.10 | -3.35 | -0.85 | -2.99 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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