Standard name | |
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Description | Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 5 | – | 0 | 0 | 3 | 1 | – | 1 | 1 | 2 | 4 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 3 | 6 | 8 |
Bud Neck | 0 | 2 | – | 0 | 3 | 2 | 4 | – | 3 | 2 | 4 | 3 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 2 | 2 |
Bud Site | 2 | 4 | – | 0 | 1 | 8 | 1 | – | 2 | 10 | 6 | 9 | 0 | 1 | 1 | 1 | 1 | 5 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | – | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 6 | – | 1 | 6 | 3 | 7 | – | 8 | 9 | 7 | 7 | 16 | 40 | 68 | 8 | 11 | 6 | 1 | 1 | 0 | 0 | 0 | 3 |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 17 | 8 | – | 3 | 4 | 2 | 7 | – | 5 | 2 | 8 | 2 | 30 | 44 | 55 | 38 | 37 | 42 | 3 | 9 | 0 | 8 | 14 | 14 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 2 |
Mitochondria | 4 | 22 | – | 0 | 1 | 1 | 1 | – | 5 | 5 | 8 | 8 | 2 | 5 | 2 | 5 | 9 | 10 | 0 | 2 | 0 | 0 | 2 | 5 |
Nucleus | 1 | 8 | – | 4 | 5 | 4 | 6 | – | 6 | 12 | 6 | 7 | 9 | 6 | 12 | 0 | 2 | 3 | 0 | 1 | 0 | 5 | 8 | 3 |
Nuclear Periphery | 0 | 1 | – | 1 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 57 | 128 | – | 10 | 46 | 32 | 62 | – | 33 | 64 | 43 | 55 | 132 | 217 | 234 | 66 | 59 | 73 | 2 | 11 | 0 | 8 | 18 | 7 |
Peroxisomes | 7 | 7 | – | 0 | 1 | 0 | 10 | – | 3 | 0 | 5 | 2 | 9 | 14 | 15 | 1 | 3 | 3 | 0 | 3 | 0 | 1 | 2 | 1 |
SpindlePole | 63 | 136 | – | 11 | 99 | 40 | 116 | – | 54 | 92 | 61 | 92 | 161 | 248 | 245 | 76 | 68 | 86 | 89 | 173 | 17 | 186 | 260 | 204 |
Vac/Vac Membrane | 0 | 6 | – | 1 | 2 | 1 | 1 | – | 2 | 0 | 1 | 0 | 1 | 3 | 5 | 11 | 7 | 18 | 0 | 2 | 0 | 4 | 3 | 5 |
Unique Cell Count | 87 | 204 | 18 | 115 | 54 | 146 | 81 | 126 | 101 | 132 | 220 | 361 | 404 | 121 | 109 | 142 | 103 | 217 | 21 | 229 | 329 | 270 | ||
Labelled Cell Count | 153 | 334 | 32 | 168 | 96 | 218 | 123 | 197 | 152 | 189 | 361 | 581 | 641 | 209 | 202 | 252 | 103 | 217 | 21 | 229 | 329 | 270 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 6.4 | – | 9.3 | 8.1 | 5.2 | 7.0 | – | 7.1 | 6.0 | 8.1 | 6.0 | 7.1 | 6.9 | 7.4 | 9.1 | 9.9 | 9.1 | 6.3 | 6.4 | 6.6 |
Std Deviation (1e-4) | 1.1 | 1.6 | – | 2.9 | 2.1 | 1.4 | 1.7 | – | 3.1 | 1.5 | 3.4 | 1.6 | 1.7 | 1.7 | 1.8 | 2.1 | 2.0 | 1.7 | 1.4 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |