Standard name
Description Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0.15 0 – 0 0.08 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – 0.06 0.05 0.06 0.05 – 0.1 0.07 0.07 0.05 0.07 0.11 0.17 0.07 0.1 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.2 0 – 0.17 0 0 0.05 – 0.06 0 0.08 0 0.14 0.12 0.14 0.31 0.34 0.3 0 0 0 0 0 0.06
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.11 – 0 0 0 0 – 0.06 0 0.08 0.06 0 0 0 0 0.08 0.07 0 0 0 0 0 0
Nucleus 0 0 – 0.22 0 0.07 0 – 0.07 0.1 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.66 0.63 – 0.56 0.4 0.59 0.42 – 0.41 0.51 0.43 0.42 0.6 0.6 0.58 0.55 0.54 0.51 0 0.05 0 0 0.06 0
Peroxisomes 0.08 0 – 0 0 0 0.07 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.72 0.67 – 0.61 0.86 0.74 0.79 – 0.67 0.73 0.6 0.7 0.73 0.69 0.61 0.63 0.62 0.61 0.87 0.8 0.84 0.81 0.79 0.76
Vac/Vac Membrane 0 0 – 0.06 0 0 0 – 0 0 0 0 0 0 0 0.09 0.06 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 1 – 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Bud 0 5 – 0 0 3 1 – 1 1 2 4 0 1 0 1 0 0 1 2 0 3 6 8
Bud Neck 0 2 – 0 3 2 4 – 3 2 4 3 0 1 1 0 0 2 0 1 0 1 2 2
Bud Site 2 4 – 0 1 8 1 – 2 10 6 9 0 1 1 1 1 5 – – – – – –
Cell Periphery 0 1 – 1 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 – 1 6 3 7 – 8 9 7 7 16 40 68 8 11 6 1 1 0 0 0 3
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 1 1 1 3 3 0 0 0 0 0 2
Endosome 17 8 – 3 4 2 7 – 5 2 8 2 30 44 55 38 37 42 3 9 0 8 14 14
Golgi 0 0 – 0 0 0 0 – 1 0 0 0 0 0 0 0 2 0 0 1 0 1 0 2
Mitochondria 4 22 – 0 1 1 1 – 5 5 8 8 2 5 2 5 9 10 0 2 0 0 2 5
Nucleus 1 8 – 4 5 4 6 – 6 12 6 7 9 6 12 0 2 3 0 1 0 5 8 3
Nuclear Periphery 0 1 – 1 0 0 0 – 0 0 1 0 1 0 2 0 0 0 0 0 0 0 0 1
Nucleolus 57 128 – 10 46 32 62 – 33 64 43 55 132 217 234 66 59 73 2 11 0 8 18 7
Peroxisomes 7 7 – 0 1 0 10 – 3 0 5 2 9 14 15 1 3 3 0 3 0 1 2 1
SpindlePole 63 136 – 11 99 40 116 – 54 92 61 92 161 248 245 76 68 86 89 173 17 186 260 204
Vac/Vac Membrane 0 6 – 1 2 1 1 – 2 0 1 0 1 3 5 11 7 18 0 2 0 4 3 5
Unique Cell Count 87 204 18 115 54 146 81 126 101 132 220 361 404 121 109 142 103 217 21 229 329 270
Labelled Cell Count 153 334 32 168 96 218 123 197 152 189 361 581 641 209 202 252 103 217 21 229 329 270


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.5 6.4 – 9.3 8.1 5.2 7.0 – 7.1 6.0 8.1 6.0 7.1 6.9 7.4 9.1 9.9 9.1 6.3 6.4 6.6
Std Deviation (1e-4) 1.1 1.6 – 2.9 2.1 1.4 1.7 – 3.1 1.5 3.4 1.6 1.7 1.7 1.8 2.1 2.0 1.7 1.4 1.4 1.8
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –