Standard name
Description Histone acetyltransferase (HAT) catalytic subunit of the SAS complex; acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family; other members are Sas4p and Sas5p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.11 0.27 0.24 0.42 0.42 0.38 0.38 0.29 0.37 0.23 0.32 – 0.41 0.43 0.16 0.14 0.08 0.06 0.08 0.12 0.18 0.22 0.22
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.56 0.06 0 0.11 0.14 0.42 0.56 0.45 0.67 0.58 0.74 0.55 – 0 0 0 0 0.08 0 0 0 0.06 0.05 0
Nucleus 0.6 0.62 0.62 0.63 0.29 0.23 0.25 0.29 0.25 0.23 0.26 0.24 – 0.46 0.41 0.68 0.7 0.67 0.61 0.61 0.49 0.34 0.22 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0.06
Vac/Vac Membrane 0.07 0.26 0.05 0 0.16 0.06 0.07 0 0.05 0 0.06 0.09 – 0.13 0.17 0.16 0.14 0.18 0.22 0.19 0.25 0.31 0.31 0.41
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 2
Bud 0 0 0 1 0 2 5 8 11 5 10 6 – 0 0 0 0 0 4 2 2 0 3 8
Bud Neck 0 0 3 0 3 4 1 9 2 4 2 4 – 2 1 0 0 1 0 0 0 0 2 2
Bud Site 0 1 0 0 0 2 7 5 10 5 11 1 – 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 – 0 0 0 2 0 0 0 0 0 0 0
Cytoplasm 34 14 46 49 149 136 151 178 136 185 100 114 – 68 77 35 30 11 25 12 30 14 36 39
Endoplasmic Reticulum 1 3 1 0 0 1 1 1 0 0 0 0 – 1 1 6 12 6 0 0 1 1 1 2
Endosome 0 2 1 5 20 3 1 1 0 2 0 2 – 5 6 8 8 5 6 0 2 1 2 1
Golgi 1 0 1 0 0 1 0 0 0 0 0 0 – 0 0 3 0 1 0 2 1 0 0 1
Mitochondria 226 7 5 22 51 136 221 212 320 290 326 196 – 1 3 6 9 10 7 5 9 4 8 5
Nucleus 241 77 105 126 103 75 99 135 121 113 115 85 – 75 74 146 150 88 264 91 123 27 36 26
Nuclear Periphery 1 0 0 0 0 1 0 0 2 1 0 0 – 0 0 0 3 0 0 1 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 1 0 0 1 0 – 0 0 0 4 2 0 0 0 0 3 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 1 6 6 2 3 7 5 7 3 5 – 0 1 3 1 2 15 1 7 1 9 10
Vac/Vac Membrane 28 33 9 6 56 21 26 18 24 16 27 31 – 21 30 34 29 23 96 29 62 25 51 73
Unique Cell Count 401 125 169 200 353 324 398 470 475 501 442 355 164 179 216 213 131 434 151 251 82 165 182
Labelled Cell Count 532 137 173 215 388 384 515 575 631 628 595 444 173 193 241 249 149 434 151 251 82 165 182


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 5.0 5.0 4.6 4.9 3.8 3.4 4.0 3.4 3.5 3.1 3.7 – 5.4 5.4 7.7 6.9 6.8 – 5.5 6.0
Std Deviation (1e-4) 0.4 1.2 1.1 1.3 1.7 1.4 1.2 1.0 1.4 0.8 0.9 1.2 – 1.9 1.9 1.7 1.7 1.7 – 2.1 2.2
Intensity Change (Log2) – – – -0.13 -0.04 -0.4 -0.54 -0.33 -0.56 -0.51 -0.69 -0.42 – 0.1 0.1 0.62 0.47 0.45 – 0.12 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm -0.6 3.3 3.2 2.5 2.5 0.4 2.3 -1.2 1.1 – 2.7 3.1 -2.6 -3.2 -4.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 2.7 0
Endosome 0 2.8 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 4.0 0 0 0 0 0 0 0 – 0 0 0 0.7 1.8
Nucleus 0.2 -7.2 -8.5 -8.5 -7.7 -8.6 -9.5 -8.3 -8.5 – -3.0 -3.9 1.1 1.7 0.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole -1.1 3.4 0.5 0.5 -0.8 -0.1 -1.3 0.4 1.4 – 2.4 3.4 3.2 2.7 3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7757 -0.1967 -0.4656 -0.5032 -0.6907 -0.3993 -0.0277 0.471 0.4125 0.2885 0.0378 0.4744 -0.4084 0.0548 -0.1115 0.1464 -0.3989 -0.2796
Actin 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0018 0.0001 0 0 0.0001 0.0001 0.0056 0 0.0002 0.0005 0.0001 0.0017
Bud 0 0 0 0.0001 0 0 0.0001 0.0017 0 0 0 0 0.0001 0 0.0004 0 0 0.0001
Bud Neck 0.0004 0.0004 0.0002 0.0006 0.0004 0.0013 0.0008 0.0003 0.0002 0.0002 0.0005 0.0042 0.0018 0.0002 0.0011 0.0005 0.0005 0.0035
Bud Periphery 0 0 0 0.0001 0 0 0.0001 0.0002 0 0 0 0 0.0001 0 0.0014 0.0001 0 0
Bud Site 0.0002 0.0003 0 0.0002 0 0 0.0002 0.0007 0.0001 0 0 0.0001 0.0005 0.0001 0.0015 0.0001 0 0.0002
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0377 0.0473 0.0146 0.0177 0.0253 0.0161 0.029 0.0348 0.0092 0.0135 0.0118 0.0176 0.0183 0.0242 0.0147 0.0153 0.0175 0.0118
Cytoplasmic Foci 0.0028 0.0013 0.0007 0.0007 0.0007 0.0005 0.0013 0.0006 0.0002 0.0001 0.0003 0.001 0.0023 0.0005 0.0014 0.0005 0.0003 0.001
Eisosomes 0.0001 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0014 0.0017 0.0003 0.0002 0.0004 0.0003 0.0012 0.0006 0.0002 0.0001 0.0001 0.0002 0.0008 0.0011 0.0003 0.0002 0.0002 0.0004
Endosome 0.0051 0.0023 0.0005 0.0003 0.0008 0.0004 0.0026 0.0005 0.0002 0.0002 0.0003 0.0007 0.0017 0.0007 0.0022 0.0006 0.0002 0.0009
Golgi 0.0002 0 0 0 0 0 0.0002 0 0 0 0 0 0.0007 0 0.0002 0.0007 0 0.0003
Lipid Particles 0.001 0.0002 0.0002 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0 0.0001 0.003 0.0001 0.002 0.0064 0 0.0025
Mitochondria 0.0005 0.0008 0.0002 0.0002 0.0003 0.0002 0.0005 0.0005 0.0003 0.0002 0.0003 0.0001 0.0005 0.0002 0.0009 0.025 0.0001 0.0001
None 0.1422 0.114 0.108 0.0915 0.0769 0.0985 0.0773 0.0783 0.0631 0.0602 0.091 0.0695 0.0489 0.0679 0.0394 0.0292 0.1219 0.0297
Nuclear Periphery 0.0194 0.0247 0.018 0.0116 0.02 0.0084 0.0148 0.0081 0.0085 0.0095 0.0072 0.0034 0.0143 0.0144 0.0118 0.0064 0.0095 0.0059
Nucleolus 0.0078 0.0079 0.0067 0.0074 0.0071 0.0127 0.0075 0.0052 0.006 0.0067 0.0104 0.0119 0.0093 0.0067 0.0074 0.0061 0.009 0.01
Nucleus 0.728 0.7609 0.8017 0.7822 0.8046 0.7853 0.8174 0.8441 0.8832 0.8678 0.8257 0.8397 0.8573 0.8531 0.8765 0.8818 0.8016 0.8786
Peroxisomes 0.0002 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0.004 0 0.0016 0.0067 0 0.0007
Punctate Nuclear 0.0514 0.036 0.0483 0.0869 0.0626 0.0755 0.0438 0.0228 0.0285 0.0411 0.0519 0.0509 0.0296 0.0297 0.0363 0.0195 0.0388 0.0522
Vacuole 0.0008 0.0014 0.0002 0.0001 0.0003 0.0002 0.0006 0.001 0.0002 0.0001 0.0002 0.0003 0.0008 0.0007 0.0005 0.0002 0.0001 0.0002
Vacuole Periphery 0.0003 0.0004 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.7319 9.6989 9.3561 9.1138 15.4737 13.0123 8.3355 5.6259 8.4646 14.8242
Translational Efficiency 0.5426 0.7738 0.4615 0.4488 0.441 0.703 0.4384 0.5745 0.4469 0.4482

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘