Standard name
Human Ortholog
Description Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.12 0 0 0.05 0 0.05 0
Bud 0 0 0.07 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.06 0 0.08 0 0.07 0.06 0.05 0.07 0.09 0.1 0.09 0.2 0.26 0.33 0.12 0.08 0 0 0 0 0 0 0
Cytoplasm 0.97 0.94 0.84 0.84 0.86 0.9 0.84 0.89 0.75 0.77 0.7 0.68 0.95 0.95 0.96 0.68 0.59 0.71 0.83 0.85 0.68 0.77 0.69 0.57
Endoplasmic Reticulum 0 0.05 0 0 0 0.07 0 0 0.05 0 0.05 0 0 0 0 0 0.05 0 0.06 0.07 0.07 0.09 0.14 0.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.05 0 0 0.05 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.22 0.07 0.07 0.18 0.09 0.2 0.23 0.25 0.31 0 0 0 0 0.08 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.14 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 7 0 4 1 1 4 5 4 1 2 3 8 2 6 4 9 4 0 4 4 15 10
Bud 4 4 5 2 2 3 6 6 6 7 6 1 6 6 1 1 0 3 0 0 0 0 3 5
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 1 4 5 7 12
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 8 2 4 3 8 10 8 9 15 8 10 53 84 117 14 3 3 1 0 2 1 2 2
Cytoplasm 252 118 64 41 77 103 152 131 103 132 54 77 251 303 341 81 22 55 223 104 67 116 213 149
Endoplasmic Reticulum 6 6 0 0 3 8 3 3 7 6 4 5 5 7 5 5 2 3 16 8 6 13 43 52
Endosome 1 0 3 0 1 0 3 1 0 3 1 0 2 1 2 12 1 4 3 0 4 3 3 8
Golgi 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 4 0 2 3 0 0 0 3 1
Mitochondria 4 0 3 11 6 8 32 13 27 40 19 35 0 0 2 3 3 1 3 0 2 0 9 5
Nucleus 0 2 1 0 1 0 0 2 1 0 1 0 0 0 0 2 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 1 0 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 1 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 0 1 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 0 3 2 1 1 0 2 1 0 2 6 5 7 0 0 2 0 1 1
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 7 1 4 3 3 1 2 3 7
Unique Cell Count 261 126 76 49 90 114 180 148 137 171 77 113 265 320 354 119 37 77 269 123 98 152 310 264
Labelled Cell Count 276 140 85 61 98 133 212 174 159 209 97 133 321 409 472 142 41 91 269 123 98 152 310 264


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 7.5 5.6 5.3 5.9 5.7 5.0 5.9 5.7 5.5 5.5 5.2 7.7 7.9 8.2 9.9 11.0 11.2 6.6 7.3 8.4
Std Deviation (1e-4) 0.6 1.3 0.8 0.8 1.1 0.9 1.1 1.1 0.8 1.3 1.1 1.1 0.8 0.9 1.0 2.0 2.9 2.3 1.0 1.4 1.5
Intensity Change (Log2) – – – -0.08 0.07 0.03 -0.16 0.06 0.01 -0.03 -0.05 -0.12 0.46 0.5 0.53 0.81 0.96 0.99 0.22 0.37 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.2 0 0 0 0 0 0 0 0 0 0 -1.1 0 0.5
Bud 0 0 0 0 0 0 0 0.3 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1.8 0 0 3.6 4.5 5.4 2.3 0 0
Cytoplasm -0.1 0.2 1.3 0 0.9 -1.5 -1.3 -2.1 -2.5 3.0 3.2 4.1 -2.5 -2.9 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1.6 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.2 0 0 2.9 1.3 3.2 3.7 3.7 4.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2734 4.852 4.0202 4.2153 3.3402 4.1639 4.0287 4.4789 4.1497 3.4719 3.9503 4.3497 3.9643 6.1259 5.2481 5.0542 6.0152 5.5771
Actin 0.0309 0.0037 0.0232 0.018 0.0132 0.0024 0.0511 0.0031 0.0181 0.0009 0.0039 0.0051 0.0001 0.007 0.0312 0.0012 0.0014 0.0036
Bud 0.0007 0.0019 0.0018 0.0007 0.0003 0.0001 0.0008 0.0002 0.0004 0.0002 0.0005 0.0002 0 0.0006 0.0105 0.0001 0.0001 0.0054
Bud Neck 0.0017 0.0018 0.0039 0.0011 0.0013 0.0037 0.0031 0.0006 0.0016 0.0007 0.001 0.0035 0.0001 0.0016 0.0016 0.0027 0.0009 0.0013
Bud Periphery 0.0013 0.0015 0.0026 0.0011 0.0004 0.0005 0.001 0.0002 0.0006 0.0004 0.0009 0.0006 0 0.0007 0.0041 0.0003 0.0001 0.004
Bud Site 0.0144 0.0477 0.0201 0.0007 0.0009 0.0003 0.0109 0.0191 0.0048 0.0002 0.0007 0.0003 0 0.0327 0.0137 0.0006 0.0001 0.0005
Cell Periphery 0.0052 0.007 0.0089 0.0014 0.0027 0.0051 0.0051 0.0062 0.0059 0.005 0.0043 0.0024 0.0064 0.0028 0.0074 0.0051 0.0012 0.0027
Cytoplasm 0.7741 0.9116 0.8683 0.8267 0.8349 0.9386 0.7214 0.8933 0.8569 0.9629 0.8252 0.9202 0.7675 0.8787 0.8236 0.9281 0.9482 0.9219
Cytoplasmic Foci 0.0266 0.0041 0.0101 0.0151 0.0139 0.0074 0.0366 0.0026 0.015 0.0016 0.0109 0.0063 0.0004 0.0046 0.0182 0.004 0.0024 0.0107
Eisosomes 0.0006 0.0005 0.0003 0.0001 0.0001 0 0.0005 0.0005 0.0001 0 0.0001 0.0001 0 0.0007 0.0003 0.0001 0 0
Endoplasmic Reticulum 0.0218 0.0037 0.0088 0.0031 0.0031 0.0129 0.0135 0.0026 0.0025 0.0112 0.0058 0.0067 0.0053 0.0219 0.0107 0.0142 0.007 0.0083
Endosome 0.0239 0.0011 0.0102 0.0131 0.0239 0.0045 0.0486 0.0004 0.0119 0.0008 0.0303 0.0023 0 0.0202 0.0103 0.0013 0.0009 0.0008
Golgi 0.0103 0.0006 0.0079 0.0138 0.0089 0.0009 0.0123 0.0005 0.0039 0.0003 0.0282 0.002 0 0.0042 0.0119 0.0005 0.0006 0.0003
Lipid Particles 0.0176 0.0007 0.0021 0.0071 0.0369 0.0006 0.0112 0.0003 0.0031 0.001 0.0055 0.0006 0.0002 0.0058 0.0103 0.0027 0.0004 0.0004
Mitochondria 0.0051 0.0019 0.0092 0.0448 0.018 0.0003 0.0043 0.0001 0.0102 0 0.0459 0.0019 0 0.0041 0.0107 0 0 0.0002
None 0.0218 0.009 0.0155 0.0078 0.0025 0.0111 0.0457 0.0676 0.0465 0.006 0.0218 0.046 0.2193 0.0043 0.0189 0.0362 0.0355 0.0364
Nuclear Periphery 0.0189 0.0002 0.0006 0.0003 0.001 0.0087 0.0036 0.0002 0.0002 0.0003 0.0002 0.0002 0.0001 0.0043 0.0008 0.0005 0.0002 0.0006
Nucleolus 0.0006 0.0001 0.0003 0 0.0005 0 0.0004 0.0001 0.0001 0 0.0001 0 0 0.0003 0.0003 0 0 0.0001
Nucleus 0.0038 0.0006 0.0009 0.0004 0.0043 0.0007 0.0012 0.0004 0.0004 0.0003 0.0003 0.0002 0 0.0008 0.0007 0.0003 0.0002 0.0009
Peroxisomes 0.0077 0.0003 0.0013 0.0423 0.0265 0.0001 0.0197 0.0001 0.0149 0 0.0103 0.0004 0 0.0016 0.0111 0 0 0.0001
Punctate Nuclear 0.0086 0.0002 0.0004 0.0009 0.0037 0.0003 0.0022 0.0001 0.0002 0 0.0002 0.0001 0 0.0007 0.001 0.0002 0 0.0012
Vacuole 0.0027 0.0016 0.0031 0.001 0.0025 0.0011 0.0054 0.0018 0.0027 0.008 0.0026 0.0007 0.0004 0.0015 0.0025 0.0017 0.0008 0.0005
Vacuole Periphery 0.0018 0.0001 0.0004 0.0004 0.0005 0.0008 0.0012 0 0.0002 0.0001 0.0013 0.0001 0 0.0007 0.0005 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.3852 8.7231 13.4437 20.3657 13.1383 20.2554 24.7635 22.2086 24.6196 18.8358
Translational Efficiency 0.9323 1.7486 1.0224 1.1375 1.4176 0.8932 0.8218 1.2094 1.0341 1.3277

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1601 1468 240 1361 1359 1588 699 852 2960 3056 939 2213

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 748.41 832.86 1255.79 1139.35 856.57 872.96 1166.63 1167.61 798.07 853.70 1189.42 1150.23
Standard Deviation 82.24 136.23 139.83 164.85 110.72 111.45 145.35 172.06 110.41 125.58 149.12 168.22
Intensity Change Log 2 — 0.154245 0.746694 0.606310 — 0.027344 0.445704 0.446915 — 0.087910 0.593894 0.523438

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000625 0.001617 0.001563 0.015516 0.000578 0.003753 0.001445 0.009520 0.000603 0.002727 0.001475 0.013207
Bud Neck 0.034464 0.024831 0.004083 0.008123 0.010096 0.036962 0.002467 0.008740 0.023276 0.031135 0.002880 0.008360
Bud Site 0.024663 0.059103 0.050309 0.122741* 0.018147 0.066846 0.029370 0.097143 0.021672 0.063126 0.034722 0.112886*
Cell Periphery 0.002176 0.000337 0.000577 0.002789 0.000851 0.000537 0.000414 0.001630 0.001568 0.000441 0.000456 0.002343
Cytoplasm 0.588291* 0.463733* 0.432308* 0.610567* 0.650138* 0.443313* 0.597497* 0.540109* 0.616686* 0.453122* 0.555276* 0.583441*
Cytoplasmic Foci 0.172428 0.232543* 0.159402 0.085290 0.178034 0.236295* 0.111242 0.124830 0.175002 0.234493* 0.123551 0.100513
Eisosomes 0.002488 0.000374 0.000063 0.000357 0.000608 0.000866 0.000071 0.000244 0.001625 0.000630 0.000069 0.000314
Endoplasmic Reticulum 0.004615 0.002389 0.002276 0.001085 0.003276 0.002623 0.001002 0.001073 0.004000 0.002511 0.001327 0.001080
Endosome 0.016759 0.051338 0.020629 0.005439 0.010443 0.042655 0.006246 0.012318 0.013859 0.046826 0.009922 0.008088
Golgi 0.015167 0.014269 0.000932 0.004886 0.006885 0.021382 0.000581 0.004587 0.011364 0.017965 0.000670 0.004771
Lipid Particles 0.010402 0.005452 0.007859 0.012859 0.004812 0.006228 0.002479 0.012702 0.007836 0.005855 0.003854 0.012799
Mitochondria 0.002736 0.002489 0.000026 0.000847 0.000528 0.004529 0.000026 0.004618 0.001722 0.003549 0.000026 0.002299
Mitotic Spindle 0.001495 0.001650 0.003127 0.008018 0.000087 0.005179 0.000988 0.003159 0.000849 0.003484 0.001535 0.006147
None 0.003786 0.000743 0.000992 0.000444 0.004509 0.000868 0.000281 0.000324 0.004118 0.000808 0.000463 0.000398
Nuclear Periphery 0.000097 0.000078 0.000238 0.000065 0.000063 0.000061 0.000114 0.000076 0.000081 0.000069 0.000145 0.000069
Nuclear Periphery Foci 0.000668 0.000561 0.020093 0.004189 0.002096 0.000664 0.008583 0.003892 0.001324 0.000614 0.011525 0.004075
Nucleolus 0.000506 0.000398 0.000072 0.000166 0.000346 0.000232 0.000068 0.000073 0.000432 0.000312 0.000069 0.000130
Nucleus 0.036928 0.010709 0.012217 0.009153 0.034310 0.009999 0.014916 0.006066 0.035726 0.010340 0.014226 0.007964
Peroxisomes 0.003595 0.007419 0.001007 0.005489 0.001475 0.009807 0.000737 0.006784 0.002622 0.008660 0.000806 0.005987
Vacuole 0.076755 0.115550 0.281792* 0.101186 0.072026 0.104528 0.221306* 0.159735 0.074584 0.109823 0.236766* 0.123727
Vacuole Periphery 0.001357 0.004417 0.000435 0.000791 0.000691 0.002672 0.000167 0.002377 0.001051 0.003510 0.000236 0.001401

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.58 -3.76 -12.52 -11.41 -11.69 -4.46 -4.07 -8.14 -3.74 -7.46 -5.52 -5.35 -14.82 -10.78 -13.91
Bud Neck 4.82 19.42 16.37 11.96 -7.10 -14.07 10.73 1.91 14.33 -5.38 -5.32 21.92 14.77 18.95 -10.02
Bud Site -11.85 -4.19 -21.04 -11.88 -11.99 -16.46 -3.40 -15.83 -3.77 -13.71 -19.77 -4.14 -26.44 -11.62 -21.85
Cell Periphery 12.22 10.98 -1.31 -10.35 -9.55 6.19 12.20 -2.78 -4.55 -5.86 13.19 14.35 -2.98 -11.07 -11.71
Cytoplasm 14.70 9.97 -0.81 -13.15 -9.91 25.13 9.33 11.34 -6.86 1.08 27.63 11.06 7.09 -15.26 -5.48
Cytoplasmic Foci -10.03 3.14 18.48 24.61 6.47 -9.62 14.13 9.15 17.24 -3.67 -14.02 14.33 20.07 30.85 2.21
Eisosomes 16.91 21.51 18.72 0.73 -11.44 -3.09 12.63 8.48 8.91 -7.72 12.43 23.49 19.61 7.16 -13.43
Endoplasmic Reticulum 3.09 4.21 5.83 3.38 3.55 0.93 4.55 4.34 3.45 -0.34 2.93 6.89 7.26 4.79 1.15
Endosome -13.15 -0.14 12.10 18.32 7.08 -16.72 7.89 -0.09 14.59 -4.07 -20.32 7.34 8.98 24.35 1.75
Golgi 1.08 21.32 9.20 8.28 -3.23 -12.31 19.86 3.37 11.90 -2.85 -8.27 27.02 9.14 14.17 -4.40
Lipid Particles 7.34 3.70 -2.35 -6.09 -4.50 -2.83 7.91 -4.52 -3.64 -5.99 4.59 11.19 -5.11 -6.95 -9.36
Mitochondria 0.60 7.20 4.38 4.80 -2.37 -4.63 13.29 -1.51 0.67 -1.80 -3.57 8.26 0.10 2.24 -2.32
Mitotic Spindle -0.35 -1.07 -3.12 -2.94 -1.02 -3.39 -1.32 -2.91 1.11 -2.02 -2.98 -1.34 -4.01 -1.56 -2.75
None 5.55 4.49 6.19 1.36 0.63 4.37 7.41 7.34 0.96 0.27 6.57 9.26 9.42 1.30 0.33
Nuclear Periphery 1.48 -7.91 2.51 0.80 8.63 0.69 -7.92 -1.08 -1.47 4.91 1.77 -10.31 1.08 -0.39 9.50
Nuclear Periphery Foci 1.60 -10.45 -11.97 -12.14 8.25 6.29 -10.23 -5.56 -8.71 5.33 5.96 -14.86 -12.28 -14.47 9.48
Nucleolus 0.91 5.45 3.38 2.58 -1.06 3.43 13.18 10.76 4.97 -0.45 2.01 8.21 5.05 3.41 -1.14
Nucleus 18.94 11.23 19.99 0.28 2.29 19.26 12.71 22.58 5.29 10.63 27.07 17.94 28.94 2.23 8.09
Peroxisomes -7.07 9.94 -2.79 3.31 -8.42 -14.94 9.58 -6.28 4.76 -7.48 -15.33 12.76 -6.49 6.13 -11.55
Vacuole -9.55 -14.44 -10.21 -1.32 11.44 -8.44 -21.76 -17.56 -12.38 8.07 -12.59 -25.99 -19.37 -9.58 16.02
Vacuole Periphery -8.17 10.19 3.11 8.97 -1.44 -11.44 18.30 -1.18 1.13 -1.83 -12.13 16.39 -0.00 6.08 -2.45
Standard name
Human Ortholog
Description Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Sip5

Sip5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sip5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available