Standard name
Description FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.09 0.09 0.13 0.05 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0.11 0.14 0.11 0.28 0.31 0.25 0.34 0.4 0.41 0.32 0 0 0 0.07 0.12 0.05 0 0 0 0.09 0.1 0.12
Nucleus 0.07 0.06 0 0 0 0 0 0 0 0 0.05 0 0.08 0.08 0.05 0.05 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0.79 0.84 0.87 0.84 0.75 0.69 0.7 0.69 0.61 0.59 0.61 0.67 0.75 0.75 0.68 0.7 0.64 0.76 0.87 0.83 0.92 0.8 0.78 0.73
Nucleolus 0 0 0.13 0.26 0.13 0.23 0.24 0.3 0.31 0.32 0.33 0.39 0 0 0 0.05 0.1 0.09 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.34 0.24 0.17 0.1 0.16 0.1 0.06 0.06 0.06 0.05 0 0.05 0.25 0.25 0.33 0.37 0.33 0.38 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 1 0 0 0 1 0 0 0 0 3 0 0 0 2 0 1 0 5 3
Bud 0 0 0 0 1 0 1 2 4 2 1 2 0 0 0 0 1 0 0 0 0 2 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Bud Site 0 0 0 0 2 1 3 5 2 1 5 1 2 2 3 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 2 9 2 13 10 14 11 6 11 9 7 38 38 54 8 12 5 1 0 0 2 1 1
Endoplasmic Reticulum 0 1 4 1 1 3 2 3 1 1 2 4 2 6 4 1 3 2 0 0 0 2 3 2
Endosome 3 0 13 9 16 15 12 25 3 4 1 8 2 2 6 3 4 2 5 1 5 7 20 26
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 3 8 9
Mitochondria 8 15 38 35 31 139 172 173 127 186 203 166 4 4 4 10 21 5 5 2 7 29 56 82
Nucleus 18 6 5 6 9 16 18 22 12 17 26 14 32 32 20 7 9 3 4 3 3 1 5 4
Nuclear Periphery 202 91 308 214 213 346 383 487 228 278 300 342 307 311 292 105 115 71 257 91 346 276 455 496
Nucleolus 11 4 47 65 37 114 134 209 117 151 160 199 14 14 6 7 19 8 2 0 1 2 3 5
Peroxisomes 0 0 0 0 1 0 0 1 0 1 1 1 0 0 6 0 0 0 0 0 0 1 4 9
SpindlePole 0 1 0 0 1 2 0 5 1 2 2 1 7 8 18 0 2 0 2 0 1 2 1 2
Vac/Vac Membrane 88 26 60 25 45 48 33 40 24 24 16 26 102 104 142 56 59 36 10 6 5 9 16 23
Unique Cell Count 257 108 354 254 285 501 551 704 373 469 490 514 410 415 430 150 181 94 296 110 376 343 586 678
Labelled Cell Count 333 146 484 358 370 695 772 983 525 679 726 771 510 521 558 197 247 133 296 110 376 343 586 678


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.2 9.1 9.7 9.5 8.9 7.9 7.8 8.2 7.7 7.5 7.6 8.3 8.9 8.9 9.1 9.4 10.3 13.0 9.2 10.3 10.5
Std Deviation (1e-4) 2.2 1.9 2.5 2.8 2.4 2.1 2.1 2.1 2.3 2.4 2.3 2.9 2.1 2.1 2.4 2.0 3.0 3.6 1.9 1.8 1.8
Intensity Change (Log2) -0.02 -0.11 -0.29 -0.31 -0.24 -0.33 -0.37 -0.34 -0.23 -0.12 -0.12 -0.09 -0.04 0.1 0.43 -0.08 0.09 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.4 -0.5 0 -1.1 -0.9 -0.2 -0.7 -1.3 3.9 3.8 5.1 1.6 2.3 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -0.1 1.2 -0.5 -1.3 -0.1 -2.6 -2.8 -3.9 -2.0 -3.2 -3.2 -2.1 0 -0.9 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.1 0.1 6.0 7.1 5.3 7.5 9.2 9.7 7.4 -5.9 -5.9 -6.1 -1.4 0.3 -1.6
Nucleus 0 1.5 1.7 1.7 1.7 1.6 1.9 3.0 1.3 4.1 4.1 2.6 0 0 0
Nuclear Periphery -1.0 -4.0 -6.1 -6.0 -6.3 -7.9 -8.7 -8.2 -6.8 -4.2 -4.2 -6.3 -4.5 -6.3 -2.7
Nucleolus 3.9 -0.1 3.5 4.1 5.9 5.8 6.3 6.5 8.2 -5.0 -5.1 -6.6 -2.9 -0.9 -1.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 3.9 0 0 0
Vacuole -2.5 -0.4 -3.2 -5.3 -5.9 -4.4 -5.6 -6.9 -5.8 2.7 2.7 5.1 5.0 4.1 4.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup53-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available