Standard name
Human Ortholog
Description Co-chaperone for Hsp40p; anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.06 0.09 0.07 0.07 0 0 0 0 0 0
Cytoplasm 0.7 0.69 0.81 0.78 0.78 0.75 0.81 – 0.75 0.77 0.66 0.7 0.58 0.77 0.77 0.66 0.67 0.72 0.26 0.46 0.27 0.18 0.3 0.22
Endoplasmic Reticulum 0.29 0.12 0.12 0.13 0.08 0 0 – 0 0 0 0 0.43 0.15 0.23 0.05 0.1 0 0.56 0.26 0.41 0.53 0.43 0.42
Endosome 0 0.08 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.12 0.07 0 0.29 0.13 – 0.26 0.24 0.38 0.31 0 0 0 0 0.09 0.05 0.05 0.07 0.19 0.09 0.1 0.12
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0.09 0.1 0 0.1 – 0.05 0 0 0.05 0 0 0 0.3 0.27 0.25 0 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 3 0 0 – 0 1 1 0 0 2 0 2 0 2 2 3 1 6 8 13
Bud 0 0 1 0 0 8 4 – 6 2 8 8 0 1 0 0 0 0 0 0 0 2 2 4
Bud Neck 0 0 0 0 0 0 1 – 4 3 4 0 0 0 0 0 0 0 2 1 0 3 1 2
Bud Site 0 0 0 0 0 0 0 – 0 0 3 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 3 8 1 5 – 2 5 5 8 3 15 18 14 5 6 0 1 1 1 3 4
Cytoplasm 46 18 26 104 182 155 348 – 310 314 230 310 57 201 219 101 47 58 26 37 14 23 56 47
Endoplasmic Reticulum 19 3 4 17 18 9 5 – 2 4 3 7 42 40 64 7 7 2 58 21 22 68 80 91
Endosome 1 2 0 2 8 0 6 – 0 1 3 2 1 9 6 3 3 0 1 0 1 3 5 9
Golgi 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 1 0 2 0 0 0 0 1 3
Mitochondria 0 1 4 9 4 60 56 – 108 97 132 137 0 2 0 6 6 4 5 5 10 11 17 25
Nucleus 1 0 0 2 1 2 4 – 7 3 3 6 0 0 1 0 2 3 0 1 0 1 0 0
Nuclear Periphery 0 1 0 0 0 0 1 – 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 1 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 3 – 5 6 3 4 0 2 0 4 0 0 0 0 0 1 0 1
Vac/Vac Membrane 2 2 1 12 23 7 41 – 19 16 14 20 2 9 1 45 19 20 3 6 0 3 6 7
Unique Cell Count 66 26 32 134 232 208 431 414 410 351 444 98 260 284 152 70 81 105 81 56 130 187 216
Labelled Cell Count 69 27 37 149 247 243 476 466 452 409 506 105 282 309 184 90 97 105 81 56 130 187 216


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 5.5 5.5 6.7 7.0 4.5 5.1 – 4.1 4.2 4.2 4.1 7.2 6.2 6.8 10.2 9.7 10.4 6.0 6.0 6.5
Std Deviation (1e-4) 2.4 1.3 0.9 1.5 1.5 1.1 1.4 – 1.0 1.1 1.5 1.0 1.4 1.2 1.4 3.4 2.5 2.8 1.0 1.5 1.5
Intensity Change (Log2) – – – 0.29 0.35 -0.26 -0.11 – -0.4 -0.39 -0.38 -0.4 0.4 0.19 0.32 0.91 0.84 0.93 0.15 0.13 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -0.4 -0.8 -0.1 – -0.8 -0.6 -1.8 -1.4 -2.4 -0.5 -0.5 -1.6 -1.5 -1.1
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 3.1 0 1.3 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 3.1 2.8 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7148 1.0748 0.9845 0.3379 0.7355 1.0622 1.86 2.6584 2.6132 1.7549 1.4581 2.3927 2.748 4.6414 3.6824 3.9204 2.7709 3.683
Actin 0.0162 0.0036 0.0027 0.0022 0.0159 0.0012 0.0722 0.0014 0.0047 0.0023 0.045 0.0013 0.0016 0.0003 0.0026 0.0019 0.0015 0.0006
Bud 0.0003 0.0002 0.0001 0.0001 0.0002 0 0.0005 0.0004 0.0006 0.001 0.0007 0 0 0 0.0001 0.0001 0.0001 0
Bud Neck 0.0006 0.0016 0.0005 0.0005 0.0014 0.0014 0.0039 0.0004 0.0008 0.0013 0.0028 0.0017 0.0002 0.0003 0.0004 0.0015 0.0025 0.0023
Bud Periphery 0.0003 0.0004 0.0001 0.0001 0.0006 0 0.0007 0.0002 0.0008 0.001 0.001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0
Bud Site 0.0013 0.0104 0.0018 0.0003 0.0003 0.0001 0.0056 0.003 0.0061 0.0064 0.0074 0.0001 0.0003 0.0021 0.0008 0.0003 0.0003 0.0001
Cell Periphery 0.0008 0.0012 0.001 0.0005 0.0005 0.0003 0.0008 0.0009 0.0012 0.0005 0.0005 0.0003 0.0011 0.0018 0.0013 0.0006 0.0005 0.0004
Cytoplasm 0.4399 0.4995 0.5512 0.448 0.5136 0.5133 0.3022 0.505 0.5085 0.3685 0.4197 0.4919 0.3901 0.5483 0.5481 0.4282 0.6549 0.5938
Cytoplasmic Foci 0.0441 0.0375 0.0299 0.0118 0.035 0.031 0.052 0.0464 0.0262 0.0481 0.0373 0.0227 0.0409 0.0339 0.0147 0.0231 0.0244 0.0109
Eisosomes 0.0003 0.0001 0.0001 0.0001 0.0004 0.0001 0.0006 0 0.0001 0.0001 0.0003 0 0.0002 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.2173 0.1954 0.23 0.3627 0.1827 0.257 0.3379 0.3095 0.3044 0.3503 0.2411 0.3752 0.3091 0.2029 0.2642 0.4143 0.1878 0.2522
Endosome 0.0519 0.0577 0.0442 0.0237 0.0614 0.0449 0.0601 0.0385 0.0354 0.0608 0.0591 0.0359 0.0369 0.0152 0.021 0.0298 0.0183 0.0148
Golgi 0.011 0.0047 0.0022 0.001 0.0098 0.0015 0.014 0.0036 0.004 0.0115 0.0308 0.0047 0.0038 0.001 0.0017 0.0019 0.0031 0.0006
Lipid Particles 0.0393 0.0207 0.0134 0.0055 0.0111 0.007 0.0288 0.0206 0.0174 0.012 0.0712 0.0149 0.0411 0.0206 0.0143 0.0115 0.0146 0.0026
Mitochondria 0.0037 0.0053 0.0006 0.0005 0.0024 0.0005 0.0039 0.0003 0.0159 0.0111 0.0406 0.0024 0.0005 0.0001 0.0005 0.0004 0.0003 0.0002
None 0.1147 0.1336 0.0866 0.1175 0.1256 0.1073 0.0619 0.0506 0.0525 0.0974 0.0161 0.0315 0.1492 0.1608 0.1147 0.0575 0.0834 0.1123
Nuclear Periphery 0.0159 0.012 0.0123 0.012 0.0216 0.0134 0.0134 0.0083 0.0074 0.0113 0.0075 0.0071 0.0151 0.0044 0.0064 0.013 0.0031 0.0034
Nucleolus 0.0209 0.0004 0.0071 0.0001 0.0007 0.0076 0.0037 0.0001 0.0002 0.0008 0.0004 0.0001 0.0001 0.0001 0.0004 0.0003 0 0.0001
Nucleus 0.0057 0.0039 0.0058 0.0039 0.004 0.0047 0.0045 0.0024 0.0019 0.0026 0.0022 0.0025 0.0012 0.0021 0.0031 0.005 0.0014 0.0021
Peroxisomes 0.0075 0.0006 0.0003 0.0002 0.0006 0.0004 0.0115 0.0005 0.0059 0.0012 0.0076 0.0025 0.0015 0.0001 0.0003 0.0002 0.0004 0.0001
Punctate Nuclear 0.0022 0.0034 0.0027 0.0016 0.0045 0.0023 0.0155 0.002 0.0013 0.0046 0.0019 0.0007 0.0025 0.0014 0.0016 0.0053 0.0008 0.0009
Vacuole 0.0052 0.007 0.0069 0.007 0.0059 0.0053 0.0048 0.0057 0.0043 0.0065 0.0041 0.0039 0.0041 0.0043 0.0034 0.0045 0.0022 0.0025
Vacuole Periphery 0.0009 0.0008 0.0006 0.0006 0.0017 0.0006 0.0013 0.0003 0.0005 0.0011 0.0026 0.0006 0.0007 0.0001 0.0003 0.0005 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.7709 40.9163 34.6991 38.1104 37.0899 42.478 43.5867 41.3848 33.0545 42.4977
Translational Efficiency 1.9521 1.7037 1.7977 1.4107 2.04 2.1524 1.5555 1.9868 2.153 1.7839

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1937 1051 198 1355 1896 2750 1566 1439 3833 3801 1764 2794

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 682.90 889.64 1026.35 1255.17 704.19 825.10 1113.29 1297.30 693.43 842.95 1103.53 1276.87
Standard Deviation 83.07 122.24 132.10 214.32 92.94 109.33 170.01 212.03 88.73 116.67 168.43 214.18
Intensity Change Log 2 — 0.381547 0.587777 0.878137 — 0.228604 0.660793 0.881475 — 0.305928 0.625307 0.879833

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000541 0.001691 0.006402 0.009456 0.000316 0.001004 0.006422 0.007431 0.000430 0.001194 0.006420 0.008413
Bud Neck 0.013646 0.008110 0.008798 0.016296 0.008102 0.023154 0.010184 0.015126 0.010904 0.018994 0.010028 0.015694
Bud Site 0.009250 0.014455 0.026356 0.074769 0.002967 0.014086 0.031314 0.062727 0.006142 0.014188 0.030758 0.068567
Cell Periphery 0.001076 0.000669 0.001371 0.002346 0.000439 0.000667 0.001741 0.002380 0.000761 0.000667 0.001700 0.002363
Cytoplasm 0.379234* 0.291926* 0.207169* 0.339386* 0.407576* 0.388392* 0.410377* 0.331674* 0.393253* 0.361719* 0.387568* 0.335414*
Cytoplasmic Foci 0.199743* 0.207972* 0.059226 0.098264 0.199265* 0.130268 0.063525 0.100807 0.199507* 0.151754 0.063043 0.099574
Eisosomes 0.001552 0.000609 0.000210 0.000251 0.000559 0.000699 0.000331 0.000189 0.001061 0.000674 0.000318 0.000219
Endoplasmic Reticulum 0.091184 0.187461* 0.256956* 0.058610 0.067695 0.084723 0.137680* 0.069885 0.079565 0.113131* 0.151068* 0.064417
Endosome 0.014633 0.032092 0.025537 0.029938 0.015571 0.016477 0.015004 0.029765 0.015097 0.020795 0.016186 0.029849
Golgi 0.027166 0.023282 0.002583 0.013818 0.009650 0.010377 0.003144 0.012978 0.018502 0.013946 0.003081 0.013385
Lipid Particles 0.091883 0.071085 0.043342 0.123284* 0.076365 0.060385 0.059338 0.161266* 0.084207 0.063344 0.057543 0.142846*
Mitochondria 0.018704 0.006992 0.000247 0.001101 0.006110 0.009011 0.000541 0.001130 0.012474 0.008453 0.000508 0.001116
Mitotic Spindle 0.000207 0.002882 0.010216 0.063361 0.001738 0.003708 0.008357 0.036195 0.000965 0.003480 0.008566 0.049370
None 0.005737 0.001113 0.000470 0.001023 0.002954 0.003974 0.001126 0.000738 0.004361 0.003183 0.001052 0.000876
Nuclear Periphery 0.001779 0.001771 0.005677 0.000918 0.002493 0.002830 0.001693 0.000481 0.002132 0.002538 0.002140 0.000693
Nuclear Periphery Foci 0.002580 0.011186 0.141514 0.030609 0.004854 0.004362 0.066129 0.037533 0.003705 0.006249 0.074591 0.034175
Nucleolus 0.001089 0.000591 0.000182 0.000332 0.001233 0.001659 0.000297 0.000285 0.001160 0.001364 0.000284 0.000308
Nucleus 0.092501 0.046409 0.055950 0.020229 0.133426* 0.178239* 0.056183 0.018706 0.112745 0.141787* 0.056157 0.019445
Peroxisomes 0.007766 0.005834 0.001265 0.004033 0.004368 0.005524 0.001391 0.003550 0.006085 0.005609 0.001377 0.003784
Vacuole 0.037001 0.080643 0.145892 0.109490 0.052872 0.058774 0.124823 0.106159 0.044852 0.064821 0.127187 0.107774
Vacuole Periphery 0.002726 0.003227 0.000637 0.002486 0.001445 0.001685 0.000400 0.000994 0.002093 0.002112 0.000426 0.001718

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.82 -3.21 -10.06 -8.30 -1.21 -3.84 -7.66 -11.53 -10.02 -1.05 -5.26 -8.13 -15.05 -13.03 -1.92
Bud Neck 4.66 2.16 -1.70 -7.45 -3.70 -10.17 -2.78 -7.33 5.04 -4.69 -7.02 0.43 -5.80 3.04 -6.15
Bud Site -2.64 -3.43 -16.50 -14.15 -6.58 -8.88 -14.80 -19.45 -14.79 -8.64 -7.08 -13.31 -25.16 -20.58 -11.89
Cell Periphery 5.13 -1.82 -3.70 -5.63 -2.10 -5.88 -8.07 -9.57 -8.15 -1.88 2.15 -6.21 -8.72 -9.53 -2.02
Cytoplasm 9.77 9.90 5.17 -3.51 -6.59 2.72 0.59 7.92 6.17 6.51 5.90 1.66 9.20 4.35 5.50
Cytoplasmic Foci -1.20 18.94 19.96 16.90 -5.33 14.23 29.15 18.95 6.75 -8.99 12.56 38.29 27.50 14.97 -10.89
Eisosomes 9.83 16.36 16.87 6.58 0.23 -3.21 6.53 12.95 12.94 4.13 7.51 15.47 20.00 14.42 3.83
Endoplasmic Reticulum -10.76 -7.09 8.54 16.05 9.45 -3.60 -8.88 2.31 5.70 10.32 -7.92 -9.57 7.68 14.55 14.42
Endosome -10.29 -4.61 -8.80 2.24 -1.17 -0.85 1.07 -7.97 -7.09 -8.74 -6.78 -0.95 -11.87 -5.53 -10.52
Golgi 2.03 17.66 10.45 6.31 -6.00 -0.96 12.73 -0.54 0.06 -6.89 5.31 23.91 8.05 3.47 -9.75
Lipid Particles 4.94 9.95 -3.66 -7.02 -10.78 4.65 4.21 -10.92 -14.10 -13.45 8.00 8.06 -10.56 -15.80 -15.46
Mitochondria 6.74 14.57 14.04 5.07 -7.15 -2.92 7.32 6.28 11.47 -1.32 4.30 15.98 15.06 12.81 -2.55
Mitotic Spindle -2.71 -2.79 -14.14 -13.38 -7.94 -2.11 -4.62 -11.41 -10.73 -8.64 -3.73 -5.91 -18.09 -17.01 -13.79
None 6.86 8.20 7.47 0.71 -1.76 -1.75 5.08 6.22 7.25 2.36 2.44 8.90 9.55 6.96 1.36
Nuclear Periphery 0.03 -4.27 3.32 3.36 5.05 -1.20 3.40 9.98 10.11 7.54 -1.98 -0.11 9.27 9.75 7.52
Nuclear Periphery Foci -8.11 -11.97 -14.22 -9.17 9.25 0.88 -21.10 -15.75 -16.11 8.30 -5.48 -24.21 -21.24 -19.24 12.34
Nucleolus 3.59 7.18 6.13 3.43 -3.46 -1.20 4.34 5.80 4.57 0.53 -0.85 5.48 8.32 4.84 0.27
Nucleus 13.89 4.61 25.89 9.72 5.03 -9.39 19.72 33.22 42.57 16.23 -8.41 19.15 41.67 41.31 17.29
Peroxisomes 3.06 13.27 8.37 3.88 -5.71 -2.75 10.01 2.50 4.84 -5.56 1.35 17.45 7.97 6.16 -8.37
Vacuole -10.99 -10.15 -20.42 -9.94 1.74 -2.26 -19.91 -17.25 -15.76 2.14 -9.54 -24.54 -26.80 -19.54 1.78
Vacuole Periphery -1.20 7.04 1.84 2.40 -3.16 -1.49 9.10 4.00 5.78 -4.63 -0.07 14.93 3.28 3.18 -5.28
Standard name
Human Ortholog
Description Co-chaperone for Hsp40p; anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
Localization
Cell Percentages ER (57%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Hlj1

Hlj1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hlj1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available