Standard name | |
---|---|
Human Ortholog | |
Description | Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 91 | 74 | 24 | 3 | 0 | 2 | 0 | 4 | – | 1 | 1 | 0 | 20 | 20 | 7 | 1 | 5 | 1 | 120 | 54 | 122 | 79 | 112 | 87 |
Bud | 13 | 19 | 48 | 10 | 15 | 40 | 44 | 55 | – | 33 | 57 | 72 | 5 | 5 | 2 | 0 | 1 | 2 | 28 | 26 | 23 | 54 | 57 | 36 |
Bud Neck | 1 | 2 | 4 | 1 | 0 | 0 | 0 | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 26 | 32 | 23 | 28 | 24 | 23 |
Bud Site | 7 | 6 | 11 | 4 | 4 | 10 | 7 | 5 | – | 16 | 10 | 6 | 0 | 0 | 0 | 0 | 1 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 5 | 1 | 0 | 1 | 2 | 0 | – | 2 | 1 | 2 | 2 | 4 | 1 | 2 | 3 | 0 | 1 | 1 | 2 | 2 | 8 | 7 |
Cytoplasm | 206 | 143 | 219 | 206 | 295 | 408 | 400 | 398 | – | 331 | 314 | 330 | 149 | 161 | 134 | 52 | 63 | 29 | 59 | 40 | 36 | 26 | 50 | 55 |
Endoplasmic Reticulum | 68 | 63 | 11 | 4 | 3 | 9 | 4 | 3 | – | 9 | 1 | 2 | 19 | 42 | 8 | 0 | 3 | 3 | 38 | 13 | 31 | 22 | 37 | 42 |
Endosome | 47 | 1 | 98 | 39 | 53 | 7 | 15 | 32 | – | 3 | 2 | 10 | 17 | 38 | 52 | 1 | 5 | 0 | 151 | 58 | 161 | 25 | 38 | 32 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 50 | 18 | 50 | 10 | 13 | 11 |
Mitochondria | 33 | 71 | 54 | 41 | 37 | 250 | 246 | 269 | – | 307 | 252 | 369 | 2 | 3 | 0 | 4 | 3 | 1 | 162 | 116 | 188 | 84 | 153 | 115 |
Nucleus | 4 | 3 | 2 | 1 | 6 | 0 | 3 | 3 | – | 0 | 5 | 1 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 |
Nucleolus | 1 | 0 | 1 | 0 | 1 | 1 | 1 | 3 | – | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 35 | 7 | 12 | 3 | 4 | 8 | – | 0 | 0 | 5 | 4 | 5 | 8 | 0 | 0 | 0 | 10 | 4 | 16 | 5 | 2 | 3 |
SpindlePole | 29 | 3 | 71 | 10 | 11 | 10 | 24 | 28 | – | 12 | 13 | 16 | 9 | 11 | 18 | 2 | 4 | 1 | 8 | 5 | 9 | 3 | 3 | 4 |
Vac/Vac Membrane | 15 | 3 | 22 | 9 | 19 | 10 | 16 | 10 | – | 9 | 7 | 12 | 2 | 9 | 15 | 1 | 1 | 0 | 67 | 58 | 48 | 31 | 76 | 73 |
Unique Cell Count | 442 | 324 | 498 | 302 | 406 | 604 | 631 | 670 | 585 | 537 | 659 | 212 | 267 | 226 | 61 | 80 | 35 | 737 | 440 | 730 | 379 | 591 | 502 | |
Labelled Cell Count | 523 | 389 | 605 | 336 | 456 | 752 | 766 | 820 | 726 | 664 | 830 | 229 | 298 | 245 | 67 | 92 | 39 | 737 | 440 | 730 | 379 | 591 | 502 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 5.1 | 5.0 | 4.7 | 4.3 | 3.5 | 3.5 | 3.9 | – | 3.1 | 3.3 | 3.4 | 6.4 | 6.4 | 6.9 | 5.1 | 5.3 | 5.7 | 6.4 | 6.4 | 6.5 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.1 | 1.5 | 1.6 | 0.9 | 1.0 | 0.9 | – | 0.8 | 0.9 | 0.8 | 0.8 | 0.8 | 0.9 | 1.3 | 1.0 | 1.6 | 1.3 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.2 | -0.52 | -0.52 | -0.36 | – | -0.66 | -0.58 | -0.56 | 0.37 | 0.36 | 0.46 | 0.04 | 0.1 | 0.2 | 0.36 | 0.36 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.3 | 1.5 | -1.1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -3.4 | -4.0 | -4.3 | -2.5 | -2.5 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6.7 | 8.7 | 7.9 | 6.5 | 5.2 | – | 4.1 | 4.7 | 2.1 | 6.4 | 4.3 | 3.8 | 6.1 | 5.8 | 4.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.1 | 7.0 | 1.0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -3.9 | -1.9 | 1.0 | -3.5 | -2.9 | -2.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -4.5 | -4.9 | -5.1 | -1.0 | -2.0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -2.8 | -3.1 | -1.8 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -3.9 | -4.3 | -2.4 | -2.4 | -2.3 | 0 |
Vacuole | -1.0 | 0.2 | -2.7 | -1.7 | -3.0 | – | -2.8 | -3.0 | -2.6 | -2.3 | -0.7 | 1.3 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0255 | 2.078 | 1.6209 | 2.0007 | 1.9182 | 1.7129 | 4.5921 | 3.8028 | 3.6271 | 3.2621 | 4.5612 | 4.0661 | 3.5083 | 3.825 | 3.6478 | 4.1425 | 3.9778 | 3.9258 |
Actin | 0.0573 | 0.0212 | 0.0145 | 0.0389 | 0.0098 | 0.0226 | 0.0503 | 0.0089 | 0.0113 | 0.0008 | 0.0554 | 0.0148 | 0.0257 | 0.0084 | 0.0095 | 0.011 | 0.0631 | 0.0315 |
Bud | 0.0069 | 0.0082 | 0.0045 | 0.0022 | 0.0263 | 0.003 | 0.003 | 0.0085 | 0.0077 | 0.0017 | 0.0036 | 0.0117 | 0.0111 | 0.0115 | 0.0055 | 0.0071 | 0.0026 | 0.0031 |
Bud Neck | 0.0222 | 0.0035 | 0.0011 | 0.0037 | 0.0016 | 0.0922 | 0.0178 | 0.0024 | 0.0044 | 0.0003 | 0.0031 | 0.1062 | 0.0346 | 0.003 | 0.0007 | 0.004 | 0.0554 | 0.1592 |
Bud Periphery | 0.0055 | 0.0086 | 0.0053 | 0.0054 | 0.0087 | 0.0055 | 0.0058 | 0.0078 | 0.0107 | 0.0026 | 0.003 | 0.0112 | 0.0175 | 0.007 | 0.0076 | 0.0078 | 0.0027 | 0.0033 |
Bud Site | 0.0625 | 0.1328 | 0.0258 | 0.0045 | 0.0077 | 0.0027 | 0.0407 | 0.1051 | 0.0341 | 0.0005 | 0.0035 | 0.0018 | 0.0621 | 0.1238 | 0.0105 | 0.0115 | 0.0229 | 0.0068 |
Cell Periphery | 0.0014 | 0.0022 | 0.0015 | 0.0009 | 0.0003 | 0.0008 | 0.0021 | 0.0016 | 0.0012 | 0.0001 | 0.0002 | 0.0018 | 0.003 | 0.002 | 0.0005 | 0.0007 | 0.0004 | 0.0009 |
Cytoplasm | 0.1899 | 0.2876 | 0.3081 | 0.2338 | 0.2381 | 0.3058 | 0.2325 | 0.3128 | 0.2622 | 0.2644 | 0.0947 | 0.3477 | 0.2786 | 0.3628 | 0.3701 | 0.2683 | 0.2154 | 0.3853 |
Cytoplasmic Foci | 0.1376 | 0.0929 | 0.1258 | 0.1247 | 0.1036 | 0.1503 | 0.1222 | 0.136 | 0.1061 | 0.1069 | 0.1277 | 0.0829 | 0.1105 | 0.087 | 0.1146 | 0.1323 | 0.1105 | 0.0876 |
Eisosomes | 0.0007 | 0.0006 | 0.0004 | 0.0004 | 0.0001 | 0.0002 | 0.0009 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0126 | 0.0079 | 0.009 | 0.0174 | 0.0054 | 0.0135 | 0.0142 | 0.0068 | 0.0089 | 0.0088 | 0.0056 | 0.0055 | 0.0186 | 0.0072 | 0.0055 | 0.0097 | 0.0095 | 0.0043 |
Endosome | 0.1428 | 0.108 | 0.1937 | 0.3009 | 0.2283 | 0.1238 | 0.1094 | 0.1025 | 0.1157 | 0.3326 | 0.178 | 0.0692 | 0.1861 | 0.1258 | 0.2182 | 0.1981 | 0.2345 | 0.0836 |
Golgi | 0.0251 | 0.0214 | 0.0284 | 0.0202 | 0.0248 | 0.0338 | 0.0195 | 0.0157 | 0.012 | 0.0127 | 0.0243 | 0.0097 | 0.0199 | 0.0241 | 0.0173 | 0.0308 | 0.0384 | 0.0166 |
Lipid Particles | 0.02 | 0.0043 | 0.0051 | 0.0085 | 0.0112 | 0.0117 | 0.016 | 0.0024 | 0.0038 | 0.0038 | 0.0264 | 0.0032 | 0.0172 | 0.0034 | 0.0061 | 0.0054 | 0.0076 | 0.0021 |
Mitochondria | 0.017 | 0.0144 | 0.0102 | 0.0075 | 0.0089 | 0.0103 | 0.0096 | 0.0102 | 0.0049 | 0.0049 | 0.0048 | 0.0081 | 0.0064 | 0.0076 | 0.0146 | 0.019 | 0.0062 | 0.0039 |
None | 0.2331 | 0.2544 | 0.2375 | 0.1824 | 0.2602 | 0.1934 | 0.2676 | 0.2521 | 0.385 | 0.2218 | 0.3883 | 0.3002 | 0.1535 | 0.2034 | 0.1744 | 0.2377 | 0.1952 | 0.1996 |
Nuclear Periphery | 0.0137 | 0.0018 | 0.0032 | 0.0077 | 0.0109 | 0.0016 | 0.026 | 0.0021 | 0.0021 | 0.0043 | 0.007 | 0.0054 | 0.0163 | 0.0019 | 0.0018 | 0.0021 | 0.001 | 0.0006 |
Nucleolus | 0.0019 | 0.0009 | 0.0007 | 0.0005 | 0.0007 | 0.0006 | 0.0017 | 0.0007 | 0.0013 | 0.0002 | 0.0005 | 0.0008 | 0.0007 | 0.0005 | 0.0004 | 0.0018 | 0.0003 | 0.0001 |
Nucleus | 0.0118 | 0.0025 | 0.0041 | 0.005 | 0.0039 | 0.0019 | 0.0167 | 0.0024 | 0.0035 | 0.0015 | 0.0017 | 0.0074 | 0.0033 | 0.0021 | 0.0017 | 0.002 | 0.0006 | 0.0007 |
Peroxisomes | 0.0161 | 0.0066 | 0.0068 | 0.0069 | 0.024 | 0.0168 | 0.0135 | 0.0067 | 0.0082 | 0.0014 | 0.0513 | 0.0021 | 0.0119 | 0.0038 | 0.0217 | 0.0307 | 0.0249 | 0.0048 |
Punctate Nuclear | 0.0117 | 0.0028 | 0.0033 | 0.0107 | 0.0081 | 0.0018 | 0.0117 | 0.0026 | 0.0048 | 0.0013 | 0.0129 | 0.0023 | 0.0016 | 0.0016 | 0.0039 | 0.0047 | 0.0016 | 0.0008 |
Vacuole | 0.008 | 0.0156 | 0.0088 | 0.0134 | 0.0142 | 0.0062 | 0.0151 | 0.0107 | 0.01 | 0.0225 | 0.0055 | 0.0062 | 0.0176 | 0.0114 | 0.0121 | 0.0122 | 0.0048 | 0.0041 |
Vacuole Periphery | 0.0022 | 0.0019 | 0.0025 | 0.0046 | 0.0031 | 0.0014 | 0.0038 | 0.0017 | 0.0018 | 0.0068 | 0.0022 | 0.0016 | 0.0034 | 0.0016 | 0.003 | 0.0029 | 0.0022 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.2071 | 11.7071 | 11.3375 | 18.7853 | 13.814 | 12.2015 | 16.5017 | 17.8065 | 18.307 | 21.3597 |
Translational Efficiency | 0.4252 | 0.335 | 0.4578 | 0.2852 | 0.3606 | 0.4212 | 0.2949 | 0.2538 | 0.3045 | 0.2606 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
301 | 915 | 1500 | 142 | 1105 | 1932 | 1609 | 1785 | 1406 | 2847 | 3109 | 1927 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 851.57 | 777.08 | 843.88 | 869.98 | 592.89 | 769.20 | 775.71 | 824.67 | 648.27 | 771.73 | 808.60 | 828.01 |
Standard Deviation | 157.03 | 112.41 | 108.52 | 120.85 | 97.09 | 93.98 | 111.21 | 105.36 | 154.75 | 100.34 | 115.07 | 107.23 |
Intensity Change Log 2 | — | -0.132062 | -0.013087 | 0.030857 | — | 0.375594 | 0.387753 | 0.476052 | — | 0.098271 | 0.165098 | 0.230457 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005350 | 0.005990 | 0.001237 | 0.002561 | 0.010942 | 0.005364 | 0.006109 | 0.006654 | 0.009745 | 0.005565 | 0.003758 | 0.006352 |
Bud Neck | 0.008319 | 0.032020 | 0.018472 | 0.018417 | 0.059393 | 0.044273 | 0.027940 | 0.024240 | 0.048459 | 0.040335 | 0.023372 | 0.023811 |
Bud Site | 0.079352 | 0.059800 | 0.028138 | 0.062313 | 0.060478 | 0.044923 | 0.071523 | 0.062991 | 0.064519 | 0.049704 | 0.050591 | 0.062941 |
Cell Periphery | 0.003237 | 0.000447 | 0.000293 | 0.000333 | 0.004028 | 0.000328 | 0.000361 | 0.000381 | 0.003859 | 0.000366 | 0.000328 | 0.000377 |
Cytoplasm | 0.299561* | 0.179244* | 0.313097* | 0.241945* | 0.130496* | 0.196483* | 0.350594* | 0.394708* | 0.166690* | 0.190942* | 0.332503* | 0.383451* |
Cytoplasmic Foci | 0.126590 | 0.421344* | 0.194383* | 0.143221 | 0.221538* | 0.387165* | 0.226772* | 0.213413* | 0.201211* | 0.398150* | 0.211145* | 0.208240* |
Eisosomes | 0.000240 | 0.000681 | 0.000157 | 0.000198 | 0.005214 | 0.001285 | 0.000216 | 0.000384 | 0.004149 | 0.001091 | 0.000188 | 0.000371 |
Endoplasmic Reticulum | 0.003543 | 0.002686 | 0.008936 | 0.004048 | 0.004497 | 0.001562 | 0.006377 | 0.006293 | 0.004293 | 0.001923 | 0.007612 | 0.006127 |
Endosome | 0.036650 | 0.077533 | 0.068553 | 0.039776 | 0.063148 | 0.076402 | 0.031547 | 0.019473 | 0.057475 | 0.076766 | 0.049401 | 0.020969 |
Golgi | 0.047339 | 0.084634 | 0.004453 | 0.001831 | 0.137582* | 0.086823 | 0.007186 | 0.006686 | 0.118263* | 0.086119 | 0.005868 | 0.006328 |
Lipid Particles | 0.004042 | 0.019643 | 0.042881 | 0.008003 | 0.054710 | 0.023927 | 0.022217 | 0.014655 | 0.043862 | 0.022550 | 0.032187 | 0.014165 |
Mitochondria | 0.010138 | 0.017923 | 0.003438 | 0.000535 | 0.151724* | 0.032776 | 0.003342 | 0.002296 | 0.121413* | 0.028003 | 0.003388 | 0.002167 |
Mitotic Spindle | 0.018761 | 0.004359 | 0.013941 | 0.007229 | 0.001697 | 0.003956 | 0.023125 | 0.004787 | 0.005350 | 0.004086 | 0.018694 | 0.004967 |
None | 0.000195 | 0.000545 | 0.007100 | 0.002128 | 0.002361 | 0.000251 | 0.001763 | 0.000611 | 0.001898 | 0.000346 | 0.004338 | 0.000723 |
Nuclear Periphery | 0.000347 | 0.000130 | 0.006552 | 0.000911 | 0.000285 | 0.000082 | 0.001073 | 0.000384 | 0.000298 | 0.000098 | 0.003716 | 0.000423 |
Nuclear Periphery Foci | 0.001624 | 0.000634 | 0.011147 | 0.004805 | 0.000616 | 0.000217 | 0.006287 | 0.002274 | 0.000832 | 0.000351 | 0.008632 | 0.002461 |
Nucleolus | 0.000200 | 0.000441 | 0.005115 | 0.001578 | 0.001148 | 0.000385 | 0.001160 | 0.000452 | 0.000945 | 0.000403 | 0.003068 | 0.000535 |
Nucleus | 0.027648 | 0.008285 | 0.102673 | 0.070223 | 0.015386 | 0.008142 | 0.067626 | 0.067180 | 0.018011 | 0.008188 | 0.084535 | 0.067404 |
Peroxisomes | 0.006505 | 0.034085 | 0.011306 | 0.023204 | 0.054260 | 0.034119 | 0.023913 | 0.019342 | 0.044037 | 0.034108 | 0.017830 | 0.019627 |
Vacuole | 0.306818* | 0.044110 | 0.155459 | 0.363893* | 0.014107 | 0.045984 | 0.119143 | 0.151741 | 0.076771 | 0.045382 | 0.136664 | 0.167374 |
Vacuole Periphery | 0.013541 | 0.005469 | 0.002669 | 0.002846 | 0.006390 | 0.005551 | 0.001726 | 0.001054 | 0.007921 | 0.005524 | 0.002181 | 0.001186 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.26 | 2.58 | 1.91 | 4.21 | -1.24 | 3.81 | 3.47 | 3.15 | -1.00 | -0.36 | 3.32 | 5.14 | 2.89 | -0.45 | -3.33 |
Bud Neck | -7.72 | -6.50 | -2.92 | 4.20 | 1.05 | 3.22 | 7.42 | 8.47 | 7.88 | 1.46 | 2.10 | 7.46 | 7.36 | 7.92 | -0.04 |
Bud Site | 0.49 | 4.23 | 1.16 | 0.96 | -2.05 | 3.22 | -0.89 | 0.38 | -3.41 | 1.43 | 2.69 | 3.20 | 0.71 | -2.17 | -2.77 |
Cell Periphery | 1.14 | 1.24 | 1.23 | 2.29 | -0.21 | 8.07 | 8.03 | 8.00 | -0.84 | -0.32 | 6.47 | 6.57 | 6.49 | 0.35 | -1.30 |
Cytoplasm | 7.20 | -0.37 | 3.26 | -1.86 | 4.20 | -9.44 | -24.49 | -31.03 | -23.84 | -4.83 | -3.54 | -21.33 | -24.85 | -25.04 | -6.28 |
Cytoplasmic Foci | -27.86 | -8.60 | -0.70 | 22.12 | 5.85 | -22.28 | 0.36 | 2.10 | 25.82 | 1.74 | -31.40 | -1.36 | -0.30 | 31.75 | 1.05 |
Eisosomes | -6.81 | 1.63 | 0.80 | 7.57 | -0.92 | 7.86 | 10.25 | 9.94 | 8.13 | -6.54 | 7.73 | 10.22 | 9.78 | 9.17 | -8.26 |
Endoplasmic Reticulum | 0.21 | -6.80 | -1.43 | -2.09 | 5.94 | 3.35 | -2.13 | -1.42 | -7.52 | 0.80 | 3.11 | -4.52 | -1.93 | -7.06 | 2.87 |
Endosome | -10.10 | -8.91 | -0.82 | 7.01 | 5.64 | -3.52 | 10.07 | 14.52 | 22.77 | 6.20 | -6.68 | 2.86 | 13.87 | 25.79 | 14.64 |
Golgi | -5.76 | 4.01 | 4.39 | 18.89 | 2.83 | 8.49 | 25.01 | 25.13 | 26.59 | 0.41 | 5.75 | 24.16 | 24.04 | 32.00 | -0.60 |
Lipid Particles | -8.82 | -14.89 | -2.68 | 5.80 | 12.36 | 8.90 | 9.37 | 12.13 | 6.39 | 4.67 | 7.61 | 4.30 | 11.03 | 6.86 | 10.91 |
Mitochondria | -3.34 | 1.50 | 2.58 | 11.05 | 7.61 | 18.67* | 24.34 | 24.53 | 16.27 | 2.31 | 17.67 | 23.14 | 23.39 | 18.74 | 3.74 |
Mitotic Spindle | 2.09 | -0.03 | 0.04 | -2.13 | 0.09 | -2.57 | -9.82 | -3.60 | -0.95 | 8.21 | 0.30 | -8.37 | -0.84 | -1.60 | 8.74 |
None | -1.47 | -5.12 | -1.70 | -1.16 | 2.99 | 2.58 | 0.78 | 2.17 | -3.62 | 1.97 | 2.33 | -2.26 | 1.89 | -1.94 | 4.99 |
Nuclear Periphery | 4.34 | -8.40 | -4.82 | -6.56 | 7.19 | 2.21 | -3.59 | -1.35 | -9.56 | 3.28 | 2.81 | -9.06 | -2.09 | -10.69 | 8.75 |
Nuclear Periphery Foci | 3.61 | -10.79 | -2.78 | -3.30 | 2.44 | 1.79 | -8.63 | -5.57 | -9.62 | 6.33 | 2.53 | -14.09 | -6.06 | -9.30 | 10.72 |
Nucleolus | -2.73 | -9.81 | -2.50 | -1.85 | 5.24 | 2.13 | -0.34 | 2.09 | -0.33 | 4.19 | 1.88 | -5.73 | 1.60 | -0.86 | 9.63 |
Nucleus | 5.55 | -13.46 | -3.47 | -6.41 | 4.38 | 2.80 | -14.44 | -14.72 | -23.76 | 0.90 | 4.68 | -21.06 | -15.16 | -24.71 | 5.74 |
Peroxisomes | -11.93 | -4.27 | -2.21 | 2.87 | -1.16 | 6.92 | 11.10 | 13.03 | 8.46 | 2.39 | 4.06 | 12.07 | 10.88 | 9.53 | -0.86 |
Vacuole | 18.65 | 11.50 | -2.29 | -15.64 | -10.77 | -14.07 | -28.11 | -34.20 | -25.96 | -5.12 | 7.77 | -9.84 | -14.06 | -28.90 | -6.86 |
Vacuole Periphery | 1.38 | 2.35 | 2.41 | 2.66 | 0.57 | 1.49 | 10.96 | 13.06 | 10.01 | 2.15 | 1.99 | 6.86 | 8.10 | 11.20 | 4.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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