Standard name
Human Ortholog
Description Transcription factor for glycerol biosynthetic genes; required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.08 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.08 0.08 0.08 0.08 0.07 0.05 0.06 0 0 0.1 0.15 0.14 0.12 0.16 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.24 0.21 0.19 0.11 0.08 0.19 0.14 0.17 0.24 0.21 0.23
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.29 0.47 0.06 0.17 0.16 0.43 0.52 0.27 0.68 0.65 0.7 0.41 0.36 0.07 0.12 0.08 0.11 0.14 0 0 0.06 0.06 0.06 0.06
Nucleus 0.59 0.45 0.59 0.53 0.5 0.45 0.46 0.55 0.44 0.39 0.4 0.51 0.49 0.42 0.42 0.35 0.44 0.47 0.31 0.29 0.18 0.12 0.11 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.05 0 0 0.05 0 0.05 0.07 0.11 0.09 0.14 0.12 0.17 0.15 0.16 0.23 0.16 0.08 0.15 0.17 0.16 0.11 0.14 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.09 0.08 0.33 0.38 0.3 0.23 0.17 0.24 0.13 0.12 0.07 0.15 0.06 0.12 0.09 0.23 0.09 0.13 0.17 0.13 0.22 0.23 0.23 0.23
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0.07 0.16 0.05 0.11 0.06 0.11 0.09 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 2 1
Bud 1 4 3 8 5 5 9 12 21 24 20 13 2 0 0 0 0 0 0 2 0 2 8 9
Bud Neck 1 2 23 4 17 10 17 16 14 13 10 12 0 0 0 0 0 0 0 2 2 3 3 7
Bud Site 2 0 2 3 7 3 13 11 29 24 19 15 0 1 2 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 15 12 19 20 24 36 26 28 32 21 12 26 40 40 3 14 10 2 11 2 0 2 1
Endoplasmic Reticulum 0 2 3 0 0 0 2 2 1 0 0 0 1 0 0 1 1 2 1 0 0 1 4 3
Endosome 5 4 0 7 10 3 6 1 2 0 5 7 7 65 59 5 10 7 44 44 68 57 67 61
Golgi 1 0 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 2 1 3 1
Mitochondria 47 114 18 41 41 135 236 107 359 367 429 212 99 20 35 2 10 12 5 13 24 15 20 15
Nucleus 94 107 164 128 130 141 211 218 230 224 242 260 133 114 118 9 39 39 69 91 71 30 33 20
Nuclear Periphery 2 2 3 3 1 1 1 4 7 9 4 6 7 3 2 0 2 2 7 4 15 5 8 9
Nucleolus 19 13 2 8 14 11 25 26 57 50 84 62 45 42 45 6 14 7 33 52 65 28 43 33
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 1 3 1 0 0 1 0 1 1 2 3 2
SpindlePole 15 20 93 90 77 72 76 97 66 66 41 77 15 33 24 6 8 11 39 42 88 55 73 62
Vac/Vac Membrane 6 14 8 2 5 4 4 3 1 7 7 3 4 15 14 2 6 13 12 35 24 27 29 22
Unique Cell Count 160 240 279 240 261 316 456 398 526 568 612 511 272 271 281 26 89 83 229 314 400 244 319 271
Labelled Cell Count 197 298 332 313 329 409 637 523 816 817 884 679 340 336 340 35 104 105 229 314 400 244 319 271


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.1 5.0 4.7 5.1 3.9 4.3 4.5 3.7 3.6 3.5 4.2 4.5 4.8 4.6 7.0 7.5 6.9 4.4 4.5 4.7
Std Deviation (1e-4) 0.6 0.7 0.6 1.3 1.2 0.9 1.1 1.1 0.7 0.6 0.7 0.9 1.0 1.3 1.0 1.6 1.4 1.5 1.7 1.6 1.6
Intensity Change (Log2) – – – -0.08 0.02 -0.34 -0.23 -0.14 -0.43 -0.47 -0.49 -0.23 -0.15 -0.07 -0.1 0.5 0.6 0.47 -0.17 -0.15 -0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8853 0.2889 0.2082 0.4156 0.1724 0.2652 0.897 0.9082 0.921 0.5937 0.7902 0.5637 -0.0009 -0.0178 -0.2722 0.8386 -0.5399 0.0749
Actin 0.0199 0.0003 0.0041 0.0376 0.0007 0.0003 0.0132 0.0004 0.0117 0.0034 0.0044 0.0002 0.024 0.0004 0.0012 0.1168 0.0011 0.0155
Bud 0.0007 0.0001 0.0002 0.0004 0.0001 0 0.0009 0.0002 0.0006 0.0002 0.0003 0.0001 0.0006 0.0002 0.0001 0.0008 0.0001 0.0003
Bud Neck 0.0049 0.001 0.0008 0.0022 0.002 0.0029 0.0035 0.0012 0.0035 0.0056 0.0017 0.004 0.0033 0.0014 0.001 0.0034 0.0011 0.0077
Bud Periphery 0.0024 0.0004 0.0004 0.0007 0.0001 0.0001 0.0012 0.0002 0.0017 0.0004 0.0007 0.0003 0.0009 0.0005 0.0003 0.0012 0.0003 0.0009
Bud Site 0.0082 0.0018 0.001 0.0012 0.0004 0.0001 0.0104 0.0012 0.0033 0.0019 0.0016 0.0003 0.0132 0.0009 0.0006 0.0067 0.0003 0.0009
Cell Periphery 0.0006 0.0002 0.0002 0.0001 0.0001 0 0.0003 0 0.0004 0.0002 0.0003 0 0.0004 0.0001 0 0.0003 0 0.0001
Cytoplasm 0.0147 0.0023 0.0019 0.001 0.0059 0.0068 0.0029 0.0017 0.0032 0.0005 0.0021 0.0003 0.0074 0.0033 0.0012 0.0009 0.0004 0.0039
Cytoplasmic Foci 0.0182 0.0003 0.0017 0.0049 0.0135 0.0002 0.014 0.0004 0.0067 0.0096 0.0193 0.0001 0.0256 0.0007 0.0012 0.0017 0.0002 0.0043
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0.0001 0.0002 0 0.0007 0 0 0.0014 0 0.0001
Endoplasmic Reticulum 0.0053 0.0005 0.0058 0.001 0.0012 0.0021 0.0021 0.0004 0.0011 0.0003 0.0074 0.0001 0.0022 0.0003 0.0004 0.0008 0.0001 0.0003
Endosome 0.0256 0.0008 0.0094 0.0036 0.0227 0.0007 0.0235 0.0008 0.0138 0.0187 0.0452 0.0003 0.0182 0.0007 0.0009 0.002 0.0004 0.0041
Golgi 0.0056 0.0003 0.004 0.0037 0.0013 0.0001 0.0141 0.0002 0.0048 0.0102 0.0298 0.0001 0.0056 0.0002 0.0004 0.0029 0.0001 0.0094
Lipid Particles 0.0152 0.0021 0.0145 0.0207 0.0125 0.0009 0.037 0.001 0.0173 0.0178 0.0648 0.0004 0.0231 0.0005 0.0027 0.0017 0.0001 0.0077
Mitochondria 0.01 0.0012 0.0053 0.0021 0.0017 0.0002 0.0071 0.0005 0.0137 0.0047 0.0306 0.0004 0.0027 0.0005 0.0012 0.0042 0.0008 0.0036
None 0.0034 0.0004 0.0051 0.0009 0.0099 0.0009 0.0022 0.0011 0.0017 0.0004 0.0014 0.0003 0.03 0.0144 0.003 0.0014 0.0003 0.0071
Nuclear Periphery 0.0501 0.032 0.0686 0.1048 0.0553 0.038 0.0748 0.0699 0.0916 0.1886 0.1785 0.0272 0.0427 0.0643 0.1126 0.1382 0.1364 0.0308
Nucleolus 0.0818 0.0865 0.0961 0.0671 0.21 0.1797 0.1036 0.1242 0.1357 0.1111 0.2326 0.2041 0.12 0.1623 0.0939 0.1371 0.101 0.2088
Nucleus 0.5979 0.7728 0.6171 0.5864 0.5358 0.6851 0.5058 0.57 0.4633 0.316 0.1904 0.6385 0.4961 0.5361 0.5463 0.3714 0.5642 0.5499
Peroxisomes 0.0119 0.0007 0.0034 0.0085 0.0016 0.0002 0.0085 0.0001 0.0034 0.0086 0.0099 0.0001 0.0116 0.0001 0.0004 0.0016 0.0001 0.0123
Punctate Nuclear 0.1115 0.0944 0.1565 0.1512 0.1226 0.0808 0.1702 0.225 0.2171 0.2985 0.1732 0.1228 0.1686 0.2116 0.2307 0.2039 0.1921 0.1302
Vacuole 0.0081 0.0014 0.0017 0.0005 0.0017 0.0004 0.0027 0.0008 0.0029 0.0019 0.003 0.0003 0.0019 0.0005 0.0003 0.0006 0.0002 0.0014
Vacuole Periphery 0.0039 0.0006 0.002 0.0011 0.0008 0.0003 0.002 0.0007 0.0023 0.0015 0.0027 0.0002 0.0012 0.0008 0.0016 0.0012 0.0006 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4938 5.4799 11.1823 11.6341 9.9348 9.1899 14.5443 10.4397 13.5016 12.7374
Translational Efficiency 0.7663 1.7047 0.7703 0.7357 0.7581 1.0427 0.65 0.7591 0.7066 0.8354

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
863 1202 88 1240 2006 1564 1234 216 2869 2766 1322 1456

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 661.50 781.62 1019.63 872.15 685.41 740.23 898.32 904.30 678.22 758.22 906.40 876.92
Standard Deviation 656.59 105.35 113.35 109.95 73.84 91.33 112.47 115.90 365.53 99.80 116.52 111.44
Intensity Change Log 2 — 0.240726 0.624233 0.398835 — 0.111006 0.390262 0.399834 — 0.176173 0.509912 0.399344

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000119 0.001025 0.004190 0.002479 0.000217 0.001017 0.001236 0.001692 0.000187 0.001021 0.001432 0.002362
Bud Neck 0.005106 0.005457 0.009357 0.019351 0.004261 0.005493 0.009949 0.011362 0.004515 0.005477 0.009909 0.018166
Bud Site 0.001319 0.004638 0.004563 0.019369 0.000904 0.001563 0.002856 0.016390 0.001029 0.002899 0.002970 0.018927
Cell Periphery 0.000283 0.000165 0.000222 0.000200 0.000108 0.000134 0.000140 0.000119 0.000160 0.000147 0.000146 0.000188
Cytoplasm 0.234838* 0.032834 0.037975 0.037555 0.069358 0.034838 0.022822 0.028422 0.119135* 0.033967 0.023831 0.036200
Cytoplasmic Foci 0.164702 0.011143 0.006118 0.002987 0.024281 0.023072 0.001181 0.027511 0.066520 0.017888 0.001510 0.006625
Eisosomes 0.000129 0.000074 0.000151 0.000042 0.000064 0.000084 0.000056 0.000068 0.000084 0.000080 0.000062 0.000046
Endoplasmic Reticulum 0.001353 0.004410 0.018381 0.008278 0.002016 0.004148 0.006403 0.011236 0.001817 0.004262 0.007200 0.008717
Endosome 0.006441 0.003585 0.003074 0.004829 0.001959 0.004969 0.001101 0.024357 0.003307 0.004367 0.001232 0.007726
Golgi 0.001592 0.001975 0.000663 0.004573 0.001350 0.005329 0.000290 0.028818 0.001423 0.003871 0.000315 0.008170
Lipid Particles 0.071747 0.013428 0.003666 0.001123 0.021054 0.024870 0.000849 0.002208 0.036302 0.019898 0.001037 0.001284
Mitochondria 0.013921 0.012265 0.001639 0.005146 0.010727 0.022829 0.002849 0.004349 0.011688 0.018238 0.002768 0.005028
Mitotic Spindle 0.004708 0.053748 0.118317* 0.381006* 0.021755 0.069600 0.063495 0.360039* 0.016627 0.062711 0.067144 0.377896*
None 0.035919 0.020725 0.005015 0.008187 0.022869 0.020512 0.018609 0.011126 0.026795 0.020605 0.017704 0.008623
Nuclear Periphery 0.004797 0.008557 0.002525 0.011931 0.011894 0.010351 0.003718 0.005929 0.009759 0.009571 0.003639 0.011040
Nuclear Periphery Foci 0.015532 0.042180 0.035487 0.047586 0.038820 0.042145 0.013114 0.026002 0.031815 0.042160 0.014603 0.044384
Nucleolus 0.053997 0.196322 0.056932 0.081075 0.179195 0.186859 0.120972 0.072604 0.141535 0.190971 0.116709 0.079818
Nucleus 0.369068* 0.579821* 0.677140* 0.348996* 0.583525* 0.528758* 0.723707* 0.339456* 0.519016* 0.550948* 0.720607* 0.347581*
Peroxisomes 0.010919 0.005136 0.002626 0.001023 0.004471 0.008718 0.000660 0.002127 0.006411 0.007161 0.000791 0.001187
Vacuole 0.002976 0.001758 0.011431 0.012281 0.000918 0.003330 0.005617 0.022075 0.001537 0.002647 0.006004 0.013734
Vacuole Periphery 0.000534 0.000756 0.000526 0.001982 0.000253 0.001382 0.000376 0.004109 0.000338 0.001110 0.000386 0.002298

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -11.13 -10.92 -11.53 -5.53 6.79 -6.52 -19.74 -8.77 -6.27 -3.45 -11.05 -21.84 -13.28 -7.45 -0.35
Bud Neck -1.24 -5.58 -17.02 -17.36 -3.66 -1.20 -18.27 -10.73 -9.86 -0.90 -1.88 -18.71 -20.82 -19.55 -5.95
Bud Site -2.64 -7.53 -9.01 -5.46 -0.79 -2.46 -11.36 -4.22 -4.05 -2.96 -3.30 -12.99 -10.05 -8.27 -5.86
Cell Periphery 2.10 -2.16 2.69 -0.15 4.52 -2.20 -1.83 -4.20 -2.81 -3.37 -0.12 0.01 -1.26 -1.04 -1.50
Cytoplasm 19.81 13.14 20.75 1.69 -0.03 7.33 14.19 5.00 0.56 -2.79 18.35 23.53 18.76 2.30 -3.73
Cytoplasmic Foci 24.34 25.27 26.15 7.61 1.06 1.89 16.88 8.28 6.37 -2.06 18.96 27.30 26.60 13.17 -2.77
Eisosomes 9.57 -3.15 12.88 2.89 6.71 -3.88 -1.83 -3.93 -2.59 -3.29 2.11 0.99 4.55 2.53 2.83
Endoplasmic Reticulum -10.33 -10.84 -19.41 -13.32 4.59 -8.31 -17.32 -9.48 -8.19 -5.03 -12.73 -19.84 -21.13 -16.26 -5.40
Endosome 4.26 1.01 4.26 -0.95 1.83 -3.46 1.57 -2.88 -1.86 -3.10 -0.25 5.26 -1.60 -1.18 -5.64
Golgi -0.21 1.79 0.89 0.89 0.09 -3.61 3.19 -1.16 -0.19 -1.50 -3.45 4.61 -0.45 1.88 -2.15
Lipid Particles 12.55 15.03 15.94 7.68 3.46 -1.30 13.78 11.59 10.93 -2.84 7.89 20.33 20.03 14.15 -2.63
Mitochondria 0.50 5.77 4.30 4.33 -6.21 -5.64 10.83 9.42 10.25 0.31 -4.04 11.04 9.01 10.42 -3.07
Mitotic Spindle -10.01 -4.26 -27.76* -20.48 -6.41 -7.58 -11.55 -11.43 -9.53 -7.82 -11.07 -13.32 -28.70 -22.89 -16.47
None 6.37 12.99 13.06 8.57 -1.32 1.51 9.31 5.78 4.47 -0.30 5.20 13.37 18.41 12.70 3.09
Nuclear Periphery -3.35 0.06 -10.04 -6.02 -10.05 1.47 6.24 -0.07 -1.02 -3.19 0.49 4.74 -5.80 -5.78 -9.28
Nuclear Periphery Foci -6.17 -1.72 -2.66 3.91 0.70 -0.21 8.70 4.06 4.09 -2.15 -2.96 7.48 2.33 5.00 -4.47
Nucleolus -13.48* 1.72 0.43 15.33 -1.51 2.83 9.06 14.21 12.09 7.34 -3.63 5.46 15.89 19.81 8.35
Nucleus -15.07 -8.79 -6.48 8.38 6.10 1.45 -9.43 5.07 4.31 9.59 -6.35 -15.38 3.85 8.85 16.37
Peroxisomes 6.72 7.51 11.47 7.88 5.28 -3.48 10.34 6.90 8.03 -2.43 0.91 14.66 14.88 11.83 0.90
Vacuole 2.01 -5.83 -10.47 -10.69 -1.24 -3.67 -8.30 -6.30 -6.01 -5.25 -3.29 -9.07 -12.55 -11.95 -8.45
Vacuole Periphery -1.11 -5.36 -1.66 -0.53 0.53 -4.00 -4.02 -1.80 -0.05 -1.55 -4.15 -3.15 -2.82 0.30 -2.27
Standard name
Human Ortholog
Description Transcription factor for glycerol biosynthetic genes; required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p
Localization
Cell Percentages nucleus (60%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Hot1

Hot1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hot1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available