Standard name
Human Ortholog
Description Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.78 0.92 0.85 0.86 0.9 0.84 – 0.71 0.69 0.63 0.73 0.64 0.64 0.63 0.88 0.82 0.84 0.73 0.56 0.62 0.75 0.71 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.1 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.07 0 0 0 0.09 0.16 – 0.33 0.4 0.46 0.31 0.07 0 0.05 0 0 0 0 0.06 0.09 0.13 0.13 0.1
Nucleus 0.06 0.09 0 0.1 0.08 0 0 – 0 0 0.05 0 0.31 0.3 0.28 0.05 0.07 0.05 0.05 0.09 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0.07 0 0 0 0 0 0 0.07 0.09 0.06 0 0 0
Vac/Vac Membrane 0 0.07 0 0 0 0 0 – 0 0 0 0 0 0.05 0.06 0.06 0.08 0.09 0 0.07 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 2 0 0 0 3 4 3 6 5
Bud 0 0 0 0 1 0 3 – 6 13 4 1 1 0 0 0 0 0 1 4 2 1 4 8
Bud Neck 0 1 6 2 15 6 10 – 8 7 7 9 0 0 0 1 1 0 1 3 2 1 4 5
Bud Site 0 0 0 0 0 0 2 – 1 5 4 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 1 1 0 3 – 3 0 1 4 0 0 0 2 0 3 0 1 1 3 5 6
Cytoplasm 105 176 239 157 223 260 331 – 288 281 212 250 153 234 238 230 143 158 112 148 155 188 289 284
Endoplasmic Reticulum 2 2 1 0 0 0 0 – 0 0 0 0 0 0 1 26 11 9 0 0 2 3 3 5
Endosome 0 5 1 0 1 2 0 – 2 0 0 1 0 3 2 1 4 6 3 7 4 2 2 3
Golgi 0 1 2 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 1 1 0 3 3
Mitochondria 7 16 0 5 7 26 63 – 134 160 154 106 16 8 17 4 6 3 5 16 23 32 54 40
Nucleus 7 20 8 19 22 11 16 – 18 18 18 12 73 111 107 12 12 10 7 24 10 1 0 0
Nuclear Periphery 0 1 0 0 0 0 1 – 0 0 0 0 0 1 2 1 0 0 0 0 1 0 1 0
Nucleolus 0 2 0 0 0 0 0 – 0 0 1 0 3 5 1 1 0 0 1 3 1 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
SpindlePole 0 2 5 6 5 3 9 – 15 6 8 24 0 0 3 1 2 2 10 24 15 0 4 5
Vac/Vac Membrane 2 15 4 0 2 4 5 – 1 9 0 4 4 19 24 15 14 17 3 18 11 9 17 15
Unique Cell Count 120 227 259 184 259 290 392 407 405 336 343 239 366 377 261 175 189 153 265 250 253 405 397
Labelled Cell Count 123 242 267 190 277 312 443 476 500 409 414 251 381 395 296 193 208 153 265 250 253 405 397


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.1 4.4 4.1 4.7 3.7 3.8 – 3.2 3.1 2.9 3.4 4.0 4.0 4.1 5.6 5.7 5.6 3.5 3.6 4.1
Std Deviation (1e-4) 0.5 1.0 1.0 1.3 1.1 1.5 1.3 – 1.0 0.7 0.6 0.8 1.0 1.0 1.3 1.3 1.3 1.2 1.1 1.3 1.8
Intensity Change (Log2) – – – -0.12 0.07 -0.27 -0.24 – -0.48 -0.51 -0.6 -0.38 -0.16 -0.13 -0.12 0.33 0.37 0.33 -0.34 -0.3 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.6 3.3 3.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7155 -0.5748 -0.6616 -0.4494 -0.4733 -0.4472 0.2399 -0.4801 -0.3444 -0.177 -0.1784 -0.4081 -0.6672 -0.8643 -0.4245 -0.2861 -1.0785 -0.8133
Actin 0.0127 0.0002 0.004 0.0339 0.0886 0.0202 0.0545 0.0025 0.0075 0.0716 0.001 0.0057 0.0398 0.0012 0.0367 0.001 0.0749 0.0121
Bud 0.0055 0.0001 0.0033 0.0015 0.002 0.0005 0.0023 0.0005 0.0039 0.0048 0.0004 0.0024 0.0047 0.0051 0.0012 0.0017 0.0044 0.0003
Bud Neck 0.0037 0.0004 0.0019 0.0008 0.0012 0.0044 0.0026 0.0088 0.001 0.0017 0.0005 0.0051 0.001 0.0003 0.0005 0.0013 0.0072 0.0021
Bud Periphery 0.014 0.0003 0.0078 0.0021 0.0043 0.0015 0.0033 0.0007 0.0148 0.0062 0.001 0.0034 0.006 0.0015 0.0025 0.0038 0.0071 0.0006
Bud Site 0.011 0.0019 0.0045 0.0269 0.005 0.001 0.0111 0.0055 0.0067 0.0096 0.0007 0.0026 0.0036 0.0018 0.0033 0.0204 0.0757 0.0003
Cell Periphery 0.0009 0.0001 0.0002 0.0003 0.0005 0.0002 0.0006 0.0002 0.0002 0.0003 0.0002 0.0002 0.0005 0.0001 0.0002 0.0001 0.0039 0.0001
Cytoplasm 0.0449 0.0983 0.0659 0.0351 0.0299 0.0544 0.0727 0.0863 0.0668 0.069 0.0625 0.1367 0.0832 0.0989 0.0788 0.0691 0.0238 0.0701
Cytoplasmic Foci 0.0436 0.0136 0.0143 0.0063 0.0066 0.0143 0.0585 0.0119 0.0154 0.0463 0.0281 0.0247 0.0177 0.0105 0.0116 0.0126 0.0037 0.0174
Eisosomes 0.0006 0.0001 0.0001 0.0003 0.0009 0.0003 0.0006 0.0002 0.0002 0.0004 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.001 0.0002
Endoplasmic Reticulum 0.0086 0.0034 0.0021 0.0115 0.0097 0.0045 0.0057 0.0015 0.0025 0.0012 0.0038 0.0027 0.0118 0.0018 0.0032 0.0008 0.0015 0.0013
Endosome 0.0223 0.0049 0.0033 0.0013 0.0207 0.0206 0.0446 0.0038 0.0079 0.0105 0.0791 0.0148 0.0232 0.0035 0.0041 0.0028 0.0057 0.005
Golgi 0.0104 0.0003 0.0007 0.0005 0.0149 0.0056 0.0144 0.0003 0.0053 0.01 0.0093 0.0049 0.0078 0.0053 0.0014 0.0011 0.0064 0.0012
Lipid Particles 0.0272 0.0045 0.0013 0.0016 0.0082 0.0056 0.0257 0.0013 0.0028 0.0115 0.0181 0.0111 0.0093 0.0008 0.0021 0.0004 0.0023 0.002
Mitochondria 0.0068 0.0003 0.0027 0.0009 0.0764 0.0022 0.0206 0.0025 0.0055 0.0124 0.0025 0.0206 0.0054 0.0006 0.0021 0.0011 0.0029 0.0094
None 0.634 0.6145 0.5604 0.8419 0.7122 0.7617 0.5126 0.5826 0.3803 0.7023 0.6877 0.615 0.6394 0.6976 0.3638 0.8291 0.593 0.7822
Nuclear Periphery 0.0213 0.0198 0.0136 0.0078 0.0046 0.0239 0.0187 0.004 0.0103 0.0012 0.0219 0.0134 0.0133 0.0062 0.0151 0.0021 0.0046 0.005
Nucleolus 0.0138 0.0072 0.0155 0.0012 0.0005 0.0015 0.0091 0.0084 0.0076 0.0018 0.0013 0.0052 0.0051 0.0059 0.0107 0.0005 0.0024 0.0027
Nucleus 0.0473 0.1765 0.2239 0.0076 0.0024 0.0601 0.0711 0.2041 0.4197 0.013 0.0078 0.0872 0.0783 0.1194 0.4179 0.0456 0.1628 0.0591
Peroxisomes 0.0285 0.0002 0.0042 0.0006 0.0071 0.001 0.0257 0.0006 0.0048 0.0151 0.0015 0.0039 0.0075 0.0002 0.0044 0.0019 0.0008 0.0027
Punctate Nuclear 0.0338 0.0507 0.0678 0.0168 0.0014 0.0125 0.0296 0.0722 0.0326 0.0092 0.0106 0.0344 0.0288 0.0373 0.0376 0.0038 0.0119 0.0256
Vacuole 0.0074 0.0023 0.0017 0.0006 0.0016 0.0023 0.0122 0.0022 0.0033 0.0014 0.0368 0.0043 0.0101 0.0017 0.002 0.0008 0.0035 0.0006
Vacuole Periphery 0.0017 0.0004 0.0005 0.0002 0.001 0.0016 0.0038 0.0002 0.0011 0.0004 0.0251 0.0014 0.0031 0.0003 0.0007 0.0001 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.8021 3.4655 4.5003 21.572 23.5608 23.7316 9.417 6.0602 12.6535 29.7821
Translational Efficiency 0.8422 1.0177 0.8643 0.5506 0.7333 1.2717 0.4829 0.5076 0.6264 0.7462

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1992 626 116 1580 2135 1610 1710 2537 4127 2236 1826 4117

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 626.33 724.68 877.88 833.92 653.48 700.39 769.48 829.71 640.38 707.19 776.37 831.33
Standard Deviation 73.10 87.66 102.82 114.35 75.93 89.21 92.02 113.62 75.80 89.45 96.44 113.92
Intensity Change Log 2 — 0.210421 0.487101 0.412986 — 0.100015 0.235741 0.344464 — 0.155103 0.364226 0.378405

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000023 0.000263 0.000655 0.001897 0.000061 0.000282 0.000241 0.001324 0.000043 0.000277 0.000267 0.001544
Bud Neck 0.007281 0.024136 0.002017 0.021513 0.011367 0.028108 0.005341 0.017318 0.009395 0.026996 0.005130 0.018928
Bud Site 0.001864 0.006461 0.001351 0.044699 0.002721 0.009896 0.008838 0.026227 0.002307 0.008934 0.008362 0.033316
Cell Periphery 0.000233 0.000148 0.000194 0.000251 0.000134 0.000179 0.000177 0.000174 0.000182 0.000170 0.000178 0.000203
Cytoplasm 0.509415* 0.394448* 0.159797* 0.318430* 0.461184* 0.399700* 0.313278* 0.300332* 0.484464* 0.398230* 0.303527* 0.307277*
Cytoplasmic Foci 0.084312 0.203384* 0.012346 0.014929 0.132460 0.216839* 0.010464 0.005589 0.109220 0.213072* 0.010584 0.009174
Eisosomes 0.000118 0.000128 0.000081 0.000045 0.000100 0.000160 0.000035 0.000026 0.000109 0.000151 0.000038 0.000033
Endoplasmic Reticulum 0.000362 0.001095 0.007323 0.004945 0.000619 0.000678 0.002124 0.002024 0.000495 0.000795 0.002455 0.003145
Endosome 0.001993 0.012785 0.002732 0.012203 0.004027 0.014492 0.002603 0.003935 0.003045 0.014014 0.002611 0.007108
Golgi 0.000170 0.001353 0.000094 0.006560 0.000871 0.003936 0.000200 0.001980 0.000533 0.003213 0.000193 0.003738
Lipid Particles 0.003598 0.009582 0.003360 0.001640 0.006944 0.010573 0.000993 0.000664 0.005329 0.010295 0.001143 0.001038
Mitochondria 0.001111 0.003780 0.000597 0.007833 0.003445 0.010763 0.001819 0.005188 0.002318 0.008808 0.001741 0.006203
Mitotic Spindle 0.000383 0.004392 0.000431 0.093962 0.001691 0.003831 0.010995 0.041381 0.001060 0.003988 0.010324 0.061560
None 0.014866 0.006639 0.003185 0.006678 0.010339 0.006799 0.007515 0.009622 0.012524 0.006754 0.007240 0.008492
Nuclear Periphery 0.000490 0.000939 0.001818 0.002648 0.000631 0.000569 0.001814 0.002084 0.000563 0.000672 0.001815 0.002301
Nuclear Periphery Foci 0.000258 0.001018 0.001183 0.001963 0.000602 0.000520 0.000514 0.000489 0.000436 0.000659 0.000557 0.001054
Nucleolus 0.002103 0.004163 0.001555 0.001943 0.003309 0.004268 0.001176 0.001954 0.002727 0.004238 0.001200 0.001950
Nucleus 0.350091* 0.269531* 0.767252* 0.379648* 0.334182* 0.234800* 0.601274* 0.553892* 0.341861* 0.244523* 0.611818* 0.487022*
Peroxisomes 0.001053 0.013668 0.000461 0.002198 0.003294 0.014679 0.000597 0.000815 0.002212 0.014396 0.000588 0.001346
Vacuole 0.020115 0.041341 0.033342 0.069950 0.021640 0.036945 0.028345 0.023779 0.020904 0.038176 0.028662 0.041499
Vacuole Periphery 0.000162 0.000746 0.000228 0.006066 0.000381 0.001984 0.001655 0.001204 0.000275 0.001637 0.001564 0.003070

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.18 -5.76 -5.03 -3.56 0.58 -5.39 -9.23 -3.50 -2.40 -2.04 -7.56 -11.23 -5.53 -3.82 -2.98
Bud Neck -5.78 4.62 -7.37 1.93 -10.05 -7.65 1.97 -5.17 4.25 -8.15 -10.37 0.74 -9.12 4.73 -9.45
Bud Site -4.38 -1.10 -8.98 -6.40 -8.64 -5.79 -4.21 -8.62 -3.33 -3.91 -7.13 -4.66 -12.45 -6.09 -5.80
Cell Periphery 3.23 0.15 3.05 -0.37 2.63 -3.30 -2.95 0.56 3.75 3.51 0.74 0.72 3.29 2.94 3.12
Cytoplasm 9.12 18.39 31.40 12.34 -4.32 6.66 21.79 29.02 18.36 4.20 11.63 29.49 42.99 22.23 3.74
Cytoplasmic Foci -12.11 19.54 26.22 22.21 1.38 -12.10 32.38 34.15 37.60 6.16 -18.65 40.06 42.82 43.57 5.04
Eisosomes -1.08 0.78 8.44 8.41 3.99 -6.39 7.34 9.90 18.44 5.72 -5.98 10.76 13.55 19.70 3.42
Endoplasmic Reticulum -4.24 -7.20 -14.00 -11.19 3.24 -1.05 -16.26 -17.43 -16.42 0.60 -4.30 -17.92 -20.88 -18.28 -1.92
Endosome -6.92 -2.60 -3.64 5.08 -0.76 -8.11 3.59 4.21 9.68 0.53 -10.82 1.71 0.36 10.65 -1.15
Golgi -5.83 2.45 -3.19 -0.80 -3.32 -4.78 4.64 0.27 4.67 -2.52 -5.85 4.29 -2.46 3.93 -4.06
Lipid Particles -3.00 -0.48 7.09 4.21 4.09 -3.89 11.16 12.13 11.82 5.80 -5.83 12.82 14.30 11.58 4.07
Mitochondria -3.73 0.92 -4.50 0.10 -6.06 -5.53 2.78 -0.11 5.60 -3.22 -6.82 1.35 -3.00 5.45 -4.06
Mitotic Spindle -2.24 -0.99 -12.94 -10.36 -12.85 -2.22 -5.17 -13.11 -11.95 -7.60 -3.58 -5.66 -18.52 -16.08 -11.01
None 4.85 8.35 5.42 -0.36 -8.05 2.97 3.73 0.87 -2.99 -4.61 5.58 6.72 4.46 -2.58 -4.31
Nuclear Periphery -2.36 -8.34 -22.29 -15.26 -9.24 1.03 -15.92 -22.75 -23.25 -8.40 -1.73 -17.70 -31.77 -28.57 -12.62
Nuclear Periphery Foci -2.05 -3.76 -4.36 -1.21 0.61 -0.42 -1.42 -0.09 0.38 1.43 -1.65 -2.62 -3.20 -0.69 0.36
Nucleolus -1.62 4.05 1.81 2.63 -2.55 -0.82 2.78 0.11 0.91 -2.60 -1.81 2.93 0.80 2.43 -2.11
Nucleus 6.08 -16.83 -16.20 -17.70 9.74 10.80 -32.13 -33.24 -41.73 1.77 13.20 -36.64 -36.14 -43.63 7.47
Peroxisomes -6.17 4.15 2.44 6.46 -0.93 -10.14 7.91 8.53 13.39 0.24 -12.45 7.75 8.71 14.60 -0.24
Vacuole -7.01 -3.74 -20.78 -13.77 -5.56 -7.37 -8.15 -11.02 -4.09 -1.96 -10.25 -9.40 -22.61 -13.63 -10.54
Vacuole Periphery -6.93 -3.93 -3.60 -2.22 -3.31 -3.43 -1.81 -0.99 2.80 1.21 -4.07 -2.09 -3.63 1.53 0.06
Standard name
Human Ortholog
Description Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress
Localization
Cell Percentages nucleus (22%), cytoplasm (13%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Spt21

Spt21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spt21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available