Standard name | |
---|---|
Human Ortholog | |
Description | Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p; SSO2 has a paralog, SSO1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 0 | 2 | 1 | 0 | 1 | 3 | 1 | 2 | 3 |
Bud | – | 0 | 6 | 6 | 1 | 3 | 2 | 1 | 6 | 5 | 8 | 11 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 2 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 69 | 29 | 20 | 42 | 110 | 260 | 225 | 242 | 362 | 357 | 448 | 35 | 46 | 107 | 23 | 16 | 13 | 0 | 4 | 5 | 0 | 5 | 4 |
Endoplasmic Reticulum | – | 20 | 8 | 1 | 5 | 6 | 2 | 0 | 0 | 0 | 14 | 5 | 16 | 10 | 9 | 5 | 2 | 5 | 0 | 7 | 3 | 0 | 5 | 11 |
Endosome | – | 44 | 147 | 166 | 88 | 20 | 24 | 6 | 12 | 1 | 3 | 1 | 64 | 88 | 43 | 44 | 38 | 47 | 3 | 98 | 199 | 63 | 91 | 60 |
Golgi | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 0 | 7 | 18 | 4 | 4 | 4 |
Mitochondria | – | 3 | 69 | 29 | 53 | 72 | 136 | 72 | 123 | 146 | 165 | 170 | 0 | 1 | 0 | 4 | 4 | 2 | 0 | 18 | 24 | 12 | 25 | 14 |
Nucleus | – | 0 | 0 | 1 | 9 | 4 | 6 | 5 | 4 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | – | 0 | 1 | 3 | 3 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Nucleolus | – | 0 | 6 | 8 | 7 | 3 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 4 |
SpindlePole | – | 2 | 17 | 36 | 13 | 2 | 4 | 1 | 3 | 2 | 0 | 1 | 14 | 26 | 25 | 4 | 6 | 5 | 0 | 0 | 4 | 3 | 3 | 4 |
Vac/Vac Membrane | – | 77 | 69 | 66 | 162 | 133 | 181 | 76 | 118 | 122 | 84 | 87 | 26 | 22 | 4 | 56 | 49 | 51 | 0 | 79 | 52 | 31 | 34 | 38 |
Unique Cell Count | 186 | 274 | 244 | 273 | 284 | 500 | 318 | 393 | 510 | 497 | 576 | 128 | 159 | 177 | 99 | 94 | 98 | 4 | 223 | 321 | 124 | 184 | 153 | |
Labelled Cell Count | 216 | 358 | 338 | 383 | 354 | 616 | 388 | 508 | 646 | 634 | 728 | 161 | 200 | 192 | 140 | 122 | 129 | 4 | 223 | 321 | 124 | 184 | 153 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 6.5 | 4.8 | 4.9 | 5.2 | 4.6 | 4.0 | 4.0 | 3.8 | 3.7 | 3.7 | 3.7 | 7.0 | 6.3 | 5.9 | 8.3 | 8.0 | 9.0 | 7.6 | 7.7 | 8.1 |
Std Deviation (1e-4) | – | 1.0 | 0.8 | 1.0 | 1.3 | 1.1 | 1.3 | 1.2 | 1.0 | 0.9 | 0.9 | 0.9 | 1.2 | 1.1 | 1.3 | 1.6 | 1.6 | 1.6 | 1.1 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.09 | -0.06 | -0.28 | -0.27 | -0.34 | -0.38 | -0.39 | -0.38 | 0.52 | 0.38 | 0.29 | 0.78 | 0.73 | 0.89 | 0.65 | 0.66 | 0.73 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | 1.7 | 7.7 | 11.4 | 14.7 | 13.2 | 16.1 | 16.3 | 18.5 | 4.3 | 4.9 | 11.3 | 3.1 | 1.6 | 0.7 |
Endoplasmic Reticulum | 0 | -0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 1.7 | 1.2 | 0 | 0 | 0 |
Endosome | 3.3 | -5.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.7 | 0.3 | -6.2 | -1.6 | -2.2 | -1.0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -3.9 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.2 | -6.7 | -7.3 | -4.5 | -4.4 | -5.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.8 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3.2 | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 3.4 | 2.8 | -0.8 | 0.1 | -0.4 |
Vacuole | 0.5 | 8.1 | 5.3 | 3.1 | -0.4 | 1.4 | -0.4 | -2.8 | -3.5 | -1.1 | -2.8 | -6.5 | 5.7 | 4.8 | 4.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2183 | 3.5901 | 3.151 | 2.629 | 3.2683 | 3.1158 | 4.1343 | 5.4876 | 5.028 | 4.6511 | 4.6086 | 5.1894 | 1.9115 | 3.4634 | 2.6488 | 2.7748 | 2.5479 | 2.9484 |
Actin | 0.0029 | 0.0001 | 0.0056 | 0.0352 | 0.0055 | 0.0002 | 0.0197 | 0.0001 | 0.002 | 0.0001 | 0.0096 | 0.0042 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud | 0.0026 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0002 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0004 | 0.0004 | 0.0009 | 0.0003 | 0.0003 | 0.0009 | 0.0005 | 0.003 | 0.0006 | 0.0002 | 0.0003 | 0.001 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0003 |
Bud Periphery | 0.0008 | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0062 | 0.0005 | 0.0007 | 0.0003 | 0.0003 | 0.0003 | 0.0009 | 0.0003 | 0.0045 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0058 | 0.0039 | 0.0052 | 0.0012 | 0.0028 | 0.0075 | 0.0069 | 0.0014 | 0.0027 | 0.0193 | 0.0014 | 0.0058 | 0.0028 | 0.0033 | 0.003 | 0.001 | 0.0016 | 0.0012 |
Cytoplasmic Foci | 0.0974 | 0.1374 | 0.1154 | 0.1645 | 0.0869 | 0.0843 | 0.098 | 0.1257 | 0.1369 | 0.1356 | 0.099 | 0.0688 | 0.0858 | 0.0995 | 0.1023 | 0.1025 | 0.0775 | 0.073 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0019 | 0.0018 | 0.002 | 0.0004 | 0.0042 | 0.0028 | 0.0068 | 0.0008 | 0.0028 | 0.0086 | 0.0005 | 0.0028 | 0.002 | 0.002 | 0.0016 | 0.0003 | 0.0013 | 0.0014 |
Endosome | 0.4134 | 0.4104 | 0.4348 | 0.6085 | 0.5898 | 0.4459 | 0.3604 | 0.3331 | 0.3845 | 0.4728 | 0.4773 | 0.3206 | 0.5249 | 0.4276 | 0.5313 | 0.5484 | 0.6383 | 0.4327 |
Golgi | 0.0654 | 0.0768 | 0.0815 | 0.0602 | 0.0698 | 0.0715 | 0.0612 | 0.0507 | 0.0809 | 0.0842 | 0.1234 | 0.0648 | 0.0574 | 0.0754 | 0.0874 | 0.0867 | 0.1016 | 0.0799 |
Lipid Particles | 0.3121 | 0.2848 | 0.2626 | 0.0902 | 0.1528 | 0.2858 | 0.3581 | 0.425 | 0.2827 | 0.1929 | 0.184 | 0.4455 | 0.2483 | 0.3127 | 0.2023 | 0.2169 | 0.1096 | 0.3441 |
Mitochondria | 0.0094 | 0.003 | 0.0211 | 0.001 | 0.0125 | 0.0048 | 0.0161 | 0.0018 | 0.0227 | 0.0043 | 0.0093 | 0.0122 | 0.0089 | 0.0145 | 0.0101 | 0.0039 | 0.0105 | 0.0051 |
None | 0.007 | 0.0004 | 0.0044 | 0.0001 | 0.0028 | 0.0008 | 0.0072 | 0.0002 | 0.0086 | 0.0067 | 0.0001 | 0.0009 | 0.0045 | 0.0003 | 0.0065 | 0.0001 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0148 | 0.0111 | 0.0032 | 0.0005 | 0.0142 | 0.0172 | 0.0072 | 0.0107 | 0.0077 | 0.0046 | 0.0005 | 0.0104 | 0.0135 | 0.0074 | 0.003 | 0.0004 | 0.0018 | 0.007 |
Nucleolus | 0.0015 | 0.0072 | 0.0012 | 0.0001 | 0.0004 | 0.0017 | 0.0013 | 0.0021 | 0.0011 | 0.0008 | 0.0001 | 0.0027 | 0.0012 | 0.0029 | 0.0004 | 0.0001 | 0.0001 | 0.0008 |
Nucleus | 0.0026 | 0.0017 | 0.0027 | 0.0001 | 0.001 | 0.0025 | 0.0009 | 0.0014 | 0.001 | 0.0005 | 0.0001 | 0.0015 | 0.001 | 0.0007 | 0.0004 | 0.0001 | 0.0002 | 0.0003 |
Peroxisomes | 0.0225 | 0.017 | 0.0249 | 0.0255 | 0.0202 | 0.0141 | 0.0254 | 0.0151 | 0.033 | 0.0255 | 0.0377 | 0.0259 | 0.0102 | 0.0125 | 0.0145 | 0.023 | 0.0056 | 0.0091 |
Punctate Nuclear | 0.0037 | 0.0056 | 0.0037 | 0.0005 | 0.0055 | 0.0017 | 0.0029 | 0.0026 | 0.0021 | 0.0008 | 0.0004 | 0.0017 | 0.003 | 0.002 | 0.0008 | 0.0005 | 0.0003 | 0.0009 |
Vacuole | 0.0214 | 0.0284 | 0.0206 | 0.0082 | 0.022 | 0.0392 | 0.0165 | 0.019 | 0.0174 | 0.0275 | 0.0381 | 0.0199 | 0.0234 | 0.0215 | 0.0236 | 0.0095 | 0.0298 | 0.0262 |
Vacuole Periphery | 0.0078 | 0.0088 | 0.0089 | 0.0032 | 0.0085 | 0.0175 | 0.0085 | 0.0066 | 0.0069 | 0.0152 | 0.0174 | 0.0103 | 0.0113 | 0.0169 | 0.0122 | 0.0061 | 0.0214 | 0.0175 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 65.2351 | 92.1951 | 89.019 | 82.4977 | 74.9138 | 78.2387 | 109.5035 | 122.4211 | 88.633 | 88.7204 |
Translational Efficiency | 2.7738 | 2.5383 | 2.5355 | 1.8288 | 2.6398 | 2.3048 | 2.1897 | 2.5194 | 2.3983 | 2.0601 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1857 | 2000 | 97 | 1093 | 1950 | 849 | 1391 | 1668 | 3807 | 2849 | 1488 | 2761 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 764.78 | 846.20 | 978.05 | 1018.95 | 772.98 | 865.42 | 1041.51 | 1071.86 | 768.98 | 851.93 | 1037.37 | 1050.91 |
Standard Deviation | 92.56 | 123.50 | 121.25 | 138.46 | 120.59 | 117.85 | 138.15 | 144.07 | 107.91 | 122.16 | 138.01 | 144.22 |
Intensity Change Log 2 | — | 0.145954 | 0.354863 | 0.413967 | — | 0.162969 | 0.430174 | 0.471613 | — | 0.154532 | 0.393211 | 0.443232 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000388 | 0.000499 | 0.003084 | 0.017104 | 0.000250 | 0.000473 | 0.010609 | 0.026228 | 0.000317 | 0.000491 | 0.010118 | 0.022616 |
Bud Neck | 0.004773 | 0.001551 | 0.008775 | 0.003054 | 0.004796 | 0.001688 | 0.000670 | 0.001401 | 0.004785 | 0.001592 | 0.001199 | 0.002055 |
Bud Site | 0.001845 | 0.002175 | 0.015592 | 0.015594 | 0.002081 | 0.002788 | 0.005882 | 0.009632 | 0.001966 | 0.002358 | 0.006515 | 0.011993 |
Cell Periphery | 0.000130 | 0.000037 | 0.000146 | 0.000084 | 0.000089 | 0.000135 | 0.000075 | 0.000074 | 0.000109 | 0.000066 | 0.000080 | 0.000078 |
Cytoplasm | 0.021554 | 0.010106 | 0.013185 | 0.009840 | 0.017861 | 0.005819 | 0.002222 | 0.006268 | 0.019662 | 0.008829 | 0.002936 | 0.007682 |
Cytoplasmic Foci | 0.188465* | 0.242847* | 0.178342 | 0.246982* | 0.186701* | 0.137790 | 0.231613* | 0.251521* | 0.187561* | 0.211540* | 0.228140* | 0.249724* |
Eisosomes | 0.000169 | 0.000038 | 0.000084 | 0.000064 | 0.000106 | 0.000050 | 0.000060 | 0.000087 | 0.000137 | 0.000041 | 0.000062 | 0.000078 |
Endoplasmic Reticulum | 0.007693 | 0.002475 | 0.007154 | 0.002302 | 0.006288 | 0.002588 | 0.003583 | 0.001402 | 0.006973 | 0.002509 | 0.003816 | 0.001758 |
Endosome | 0.408131* | 0.403837* | 0.378462* | 0.358609* | 0.455938* | 0.535246* | 0.409714* | 0.388409* | 0.432619* | 0.442997* | 0.407677* | 0.376612* |
Golgi | 0.093175 | 0.155697* | 0.024625 | 0.091219 | 0.085589 | 0.093544 | 0.222885* | 0.159909* | 0.089289 | 0.137175* | 0.209961* | 0.132716* |
Lipid Particles | 0.122562* | 0.041745 | 0.044837 | 0.037057 | 0.091057 | 0.045954 | 0.031720 | 0.034688 | 0.106424* | 0.042999 | 0.032575 | 0.035626 |
Mitochondria | 0.018067 | 0.014007 | 0.000930 | 0.002723 | 0.011972 | 0.013627 | 0.001955 | 0.004458 | 0.014945 | 0.013894 | 0.001889 | 0.003771 |
Mitotic Spindle | 0.001300 | 0.010977 | 0.053709 | 0.077917 | 0.002258 | 0.005945 | 0.013661 | 0.032912 | 0.001791 | 0.009477 | 0.016271 | 0.050728 |
None | 0.000130 | 0.000265 | 0.000054 | 0.000073 | 0.000517 | 0.000219 | 0.000011 | 0.000119 | 0.000328 | 0.000251 | 0.000014 | 0.000101 |
Nuclear Periphery | 0.000897 | 0.000835 | 0.001753 | 0.000781 | 0.000732 | 0.000531 | 0.000381 | 0.000483 | 0.000813 | 0.000745 | 0.000471 | 0.000601 |
Nuclear Periphery Foci | 0.001887 | 0.003652 | 0.040964 | 0.014341 | 0.003201 | 0.005807 | 0.007477 | 0.006419 | 0.002560 | 0.004294 | 0.009660 | 0.009555 |
Nucleolus | 0.000559 | 0.000713 | 0.003610 | 0.000128 | 0.001616 | 0.001152 | 0.000027 | 0.000343 | 0.001100 | 0.000844 | 0.000260 | 0.000258 |
Nucleus | 0.002704 | 0.001047 | 0.006769 | 0.000433 | 0.002937 | 0.000807 | 0.000281 | 0.000270 | 0.002823 | 0.000975 | 0.000704 | 0.000334 |
Peroxisomes | 0.008690 | 0.003596 | 0.030135 | 0.022420 | 0.006608 | 0.005199 | 0.005543 | 0.021984 | 0.007624 | 0.004074 | 0.007146 | 0.022157 |
Vacuole | 0.100100 | 0.080352 | 0.179192 | 0.093838 | 0.102402 | 0.109502 | 0.044584 | 0.046742 | 0.101279 | 0.089038 | 0.053358 | 0.065386 |
Vacuole Periphery | 0.016783 | 0.023551 | 0.008600 | 0.005435 | 0.017000 | 0.031138 | 0.007048 | 0.006652 | 0.016894 | 0.025812 | 0.007149 | 0.006170 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.48 | -2.65 | -6.81 | -6.73 | -5.25 | -2.24 | -6.79 | -9.63 | -9.54 | -4.98 | -1.04 | -6.86 | -11.73 | -11.59 | -5.15 |
Bud Neck | 5.99 | -2.38 | 2.25 | -4.05 | 3.12 | 5.39 | 7.81 | 6.08 | 0.65 | -4.51 | 8.09 | 8.97 | 6.34 | -2.57 | -4.02 |
Bud Site | -1.00 | -5.44 | -8.14 | -7.92 | -0.25 | -1.52 | -4.35 | -7.86 | -6.83 | -3.09 | -1.44 | -5.55 | -11.30 | -10.73 | -4.88 |
Cell Periphery | 4.98 | -0.59 | 1.93 | -4.56 | 2.06 | -1.76 | 1.20 | 1.32 | 2.41 | 0.14 | 3.34 | 2.48 | 2.50 | -1.55 | 0.02 |
Cytoplasm | 6.48 | 1.92 | 4.12 | -1.05 | 0.76 | 7.22 | 10.76 | 6.56 | -1.04 | -4.90 | 8.59 | 15.30 | 7.98 | -0.20 | -6.27 |
Cytoplasmic Foci | -10.54 | 0.96 | -7.57 | 0.56 | -4.16 | 8.29 | -7.51 | -10.72 | -17.17 | -2.88 | -5.88 | -7.82 | -13.19 | -7.27 | -3.23 |
Eisosomes | 11.44 | 4.68 | 8.14 | -4.19 | 1.71 | 5.72 | 4.79 | 1.95 | -4.95 | -3.72 | 13.00 | 9.00 | 7.24 | -7.92 | -2.66 |
Endoplasmic Reticulum | 6.79 | -0.19 | 6.73 | 0.15 | 3.02 | 4.97 | 3.20 | 6.65 | 3.08 | 4.28 | 8.16 | 4.62 | 9.71 | 2.16 | 4.33 |
Endosome | 0.61 | 1.90 | 6.55 | 6.40 | 0.53 | -7.33 | 5.40 | 8.35 | 13.78 | 2.91 | -1.65 | 3.61 | 9.97 | 11.12 | 4.94 |
Golgi | -12.09 | 13.33 | 1.31 | 12.33 | -11.16 | -1.46 | -22.82 | -13.84 | -10.07 | 9.67 | -12.35 | -21.85 | -10.62 | 1.73 | 13.56 |
Lipid Particles | 16.79 | 8.82 | 17.22 | 1.36 | 0.97 | 8.20 | 13.48 | 12.59 | 2.33 | -0.91 | 18.30 | 20.68 | 21.12 | 2.71 | -1.07 |
Mitochondria | 2.45 | 13.18 | 10.77 | 8.91 | -2.28 | -0.78 | 11.17 | 6.87 | 4.30 | -2.57 | 0.88 | 15.09 | 12.27 | 9.15 | -2.49 |
Mitotic Spindle | -7.84 | -4.14 | -13.61 | -11.72 | -2.03 | -2.16 | -5.69 | -11.21 | -8.75 | -6.02 | -7.53 | -7.17 | -17.46 | -14.14 | -10.36 |
None | -1.01 | 1.43 | -0.48 | 0.59 | -1.18 | 1.86 | 4.02 | 2.74 | 0.63 | -1.20 | 0.80 | 4.65 | 2.20 | 0.96 | -1.99 |
Nuclear Periphery | 0.27 | -2.42 | -0.28 | -0.51 | 2.30 | 1.48 | 2.69 | 1.62 | 0.25 | -1.18 | 0.46 | 3.29 | 1.27 | 0.61 | -2.15 |
Nuclear Periphery Foci | -3.50 | -4.45 | -7.01 | -5.98 | 2.93 | -1.82 | -3.45 | -2.68 | -0.56 | 0.58 | -2.93 | -5.76 | -7.04 | -5.17 | -0.12 |
Nucleolus | -0.79 | -1.92 | 2.35 | 2.16 | 2.08 | 0.81 | 5.61 | 3.58 | 1.17 | -1.53 | 1.03 | 3.96 | 3.89 | 1.97 | -0.01 |
Nucleus | 3.71 | -1.62 | 2.72 | 0.02 | 1.91 | 3.95 | 5.12 | 5.50 | 1.39 | -0.10 | 5.23 | 3.34 | 6.16 | 1.27 | 0.88 |
Peroxisomes | 6.98 | -3.81 | -5.00 | -7.05 | 1.23 | 1.68 | 1.26 | -7.93 | -8.53 | -8.29 | 6.73 | 0.65 | -9.12 | -11.55 | -8.77 |
Vacuole | 3.87 | -4.17 | -1.08 | -4.02 | 3.75 | -1.02 | 12.30 | 10.62 | 8.50 | -1.57 | 2.95 | 11.56 | 6.90 | 3.83 | -4.51 |
Vacuole Periphery | -4.62 | 4.06 | 11.09 | 13.98 | 1.41 | -6.10 | 11.33 | 9.89 | 10.51 | 0.51 | -7.45 | 13.28 | 14.22 | 16.82 | 1.51 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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